Rh6BG033200

At3g49720-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000825
Physical Location & Seq
Forward (+)
4968519 .. 4972132
3614 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG033200.1

Sequence Viewer

Length: 645 bp
ATGTCCAGGAGGCCAGTAAATCCTTCTAGGCGCATTGGTGATGGTGGAAGCATACCCCTTGTGGGTGCGGTGCAGGCCAAAGCACGCTCATCACCTTTACTGTCTGTAGCGCTTGTGCTTGTGGGTACAATTCTTCTTGTCTGCTATGCTTTTAGCGGGTCAGGTGGAGCGAGCAGTAAAGAGGCTGTGATTAAACTTGAAGGTGGTGTTTCTTGTACATATGAAGTTCAGAGAGCAATACCTATACTGAAGAAGGCATATGGTGATAGCATGCATAAGGTATTGCATGTAGGCCCTGAAACGTGTTCAGTGGTATCTAAATTATTAAAAGAGGAGGATACTGAAGCCTGGGGTGTGGAACCATATGACTTGGAAGATGTTGATTCAAATTGCAAGAGTCTTGTGAGCAGAGGCATTGTGCGTGCTGCTGATATAAAGTTTCCTCTGCCATACCGGGCAAAATCATTTTCTCTGGTAATAGTATCAGATGCATTAGATTACTTGTCTCCAAAGTACCTCAACAGAACTCTTCCAGAGTTAGCAAGGGTATCTGCTGATGGCGTTATTATATTCTCTGGTTATCCAGGTCAACAAAGAGCTAAAGTTGCAGAGCTATCCAAATTTGGCCGTCCAGTAAGTAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

214

Amino Acids

22.92

Weight (kDa)

9.14

Isoelectric Point (pI)

45.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 68, 156
AcoI YGGCCR 1 cut(s) 625
AcsI RAATTY 1 cut(s) 620
AcuI CTGAAG 2 cut(s) 269, 363
AfaI GTAC 3 cut(s) 127, 217, 515
AfeI AGCGCT 1 cut(s) 111
AfiI CCNNNNNNNGG 1 cut(s) 62
AflIII ACRYGT 1 cut(s) 302
AgsI TTSAA 2 cut(s) 200, 387
AjnI CCWGG 3 cut(s) 5, 347, 583
AluBI AGCT 2 cut(s) 599, 613
AluI AGCT 2 cut(s) 599, 613
Alw26I GTCTC 1 cut(s) 510
Aor51HI AGCGCT 1 cut(s) 111
AoxI GGCC 4 cut(s) 11, 75, 292, 625
ApeKI GCWGC 1 cut(s) 425
ApoI RAATTY 1 cut(s) 620
AspLEI GCGC 2 cut(s) 33, 112
AspS9I GGNCC 1 cut(s) 293
AsuC2I CCSGG 1 cut(s) 455
AsuHPI GGTGA 3 cut(s) 50, 84, 275
BbvI GCAGC 1 cut(s) 412
BccI CCATC 2 cut(s) 35, 551
BceAI ACGGC 1 cut(s) 612
BciT130I CCWGG 3 cut(s) 7, 349, 585
BciVI GTATCC 1 cut(s) 331
BcnI CCSGG 1 cut(s) 455
BcoDI GTCTC 1 cut(s) 510
BfaI CTAG 1 cut(s) 27
BfmI CTRYAG 1 cut(s) 105
BfoI RGCGCY 1 cut(s) 113
BfuI GTATCC 1 cut(s) 331
BisI GCNGC 1 cut(s) 426
BlsI GCNGC 1 cut(s) 427
Bme1390I CCNGG 4 cut(s) 7, 349, 455, 585
BmgT120I GGNCC 1 cut(s) 293
BmiI GGNNCC 1 cut(s) 360
BmrFI CCNGG 4 cut(s) 7, 349, 455, 585
BmsI GCATC 1 cut(s) 478
BpuMI CCSGG 1 cut(s) 455
BsaJI CCNNGG 1 cut(s) 348
Bsc4I CCNNNNNNNGG 1 cut(s) 62
Bse1I ACTGG 2 cut(s) 14, 632
BseBI CCWGG 3 cut(s) 7, 349, 585
BseDI CCNNGG 1 cut(s) 348
BseLI CCNNNNNNNGG 1 cut(s) 62
BseNI ACTGG 2 cut(s) 14, 632
BseRI GAGGAG 1 cut(s) 347
BseXI GCAGC 1 cut(s) 412
BsgI GTGCAG 1 cut(s) 92
BshFI GGCC 4 cut(s) 13, 77, 294, 627
BsiSI CCGG 1 cut(s) 454
BslI CCNNNNNNNGG 1 cut(s) 62
BsmAI GTCTC 1 cut(s) 510
BsnI GGCC 4 cut(s) 13, 77, 294, 627
Bsp1407I TGTACA 1 cut(s) 215
BspACI CCGC 2 cut(s) 68, 156
BspANI GGCC 4 cut(s) 13, 77, 294, 627
BspLI GGNNCC 1 cut(s) 360
BsrGI TGTACA 1 cut(s) 215
BsrI ACTGG 2 cut(s) 14, 632
BssECI CCNNGG 1 cut(s) 348
Bst2UI CCWGG 3 cut(s) 7, 349, 585
Bst4CI ACNGT 1 cut(s) 102
Bst6I CTCTTC 1 cut(s) 534
BstAUI TGTACA 1 cut(s) 215
BstC8I GCNNGC 5 cut(s) 75, 85, 172, 272, 423
BstH2I RGCGCY 1 cut(s) 113
BstHHI GCGC 2 cut(s) 33, 112
BstMAI GTCTC 1 cut(s) 510
BstMWI GCNNNNNNNGC 2 cut(s) 74, 605
BstNI CCWGG 3 cut(s) 7, 349, 585
BstNSI RCATGY 2 cut(s) 274, 290
BstSCI CCNGG 4 cut(s) 5, 347, 453, 583
BstSFI CTRYAG 1 cut(s) 105
BstV1I GCAGC 1 cut(s) 412
BsuI GTATCC 1 cut(s) 331
BsuRI GGCC 4 cut(s) 13, 77, 294, 627
BtsIMutI CAGTG 1 cut(s) 315
Cac8I GCNNGC 5 cut(s) 75, 85, 172, 272, 423
CfoI GCGC 2 cut(s) 33, 112
Cfr13I GGNCC 1 cut(s) 293
Csp6I GTAC 3 cut(s) 126, 216, 514
CviAII CATG 2 cut(s) 271, 287
CviJI RGCY 8 cut(s) 13, 77, 185, 294, 347, 599, 613, 627
CviKI_1 RGCY 8 cut(s) 13, 77, 185, 294, 347, 599, 613, 627
CviQI GTAC 3 cut(s) 126, 216, 514
EaeI YGGCCR 1 cut(s) 625
Eam1104I CTCTTC 1 cut(s) 534
EarI CTCTTC 1 cut(s) 534
Eco47III AGCGCT 1 cut(s) 111
Eco57I CTGAAG 2 cut(s) 269, 363
EcoO109I RGGNCCY 1 cut(s) 293
EcoRII CCWGG 3 cut(s) 5, 347, 583
EcoT22I ATGCAT 2 cut(s) 276, 493
FaeI CATG 2 cut(s) 274, 290
FatI CATG 2 cut(s) 270, 286
FauI CCCGC 1 cut(s) 149
FauNDI CATATG 3 cut(s) 220, 259, 364
Fnu4HI GCNGC 1 cut(s) 426
Fsp4HI GCNGC 1 cut(s) 426
FspBI CTAG 1 cut(s) 27
GlaI GCGC 2 cut(s) 32, 111
GluI GCNGC 1 cut(s) 426
HaeII RGCGCY 1 cut(s) 113
HaeIII GGCC 4 cut(s) 13, 77, 294, 627
HapII CCGG 1 cut(s) 454
HhaI GCGC 2 cut(s) 33, 112
Hin1II CATG 2 cut(s) 274, 290
Hin6I GCGC 2 cut(s) 31, 110
HinP1I GCGC 2 cut(s) 31, 110
HincII GTYRAC 1 cut(s) 590
HindII GTYRAC 1 cut(s) 590
HinfI GANTC 2 cut(s) 383, 397
HpaII CCGG 1 cut(s) 454
HphI GGTGA 3 cut(s) 50, 84, 275
Hpy166II GTNNAC 1 cut(s) 590
Hpy188I TCNGA 2 cut(s) 231, 487
Hpy188III TCNNGA 1 cut(s) 533
Hpy8I GTNNAC 1 cut(s) 590
HpyAV CCTTC 3 cut(s) 33, 194, 247
HpyCH4III ACNGT 1 cut(s) 102
HpyCH4IV ACGT 1 cut(s) 302
HpyCH4V TGCA 6 cut(s) 73, 274, 286, 393, 491, 608
HpyF10VI GCNNNNNNNGC 2 cut(s) 74, 605
HpySE526I ACGT 1 cut(s) 302
Hsp92II CATG 2 cut(s) 274, 290
HspAI GCGC 2 cut(s) 31, 110
LmnI GCTCC 1 cut(s) 167
Lsp1109I GCAGC 1 cut(s) 412
LweI GCATC 1 cut(s) 478
MaeI CTAG 1 cut(s) 27
MaeII ACGT 1 cut(s) 302
MaeIII GTNAC 1 cut(s) 638
MboII GAAGA 4 cut(s) 125, 262, 386, 521
MluCI AATT 4 cut(s) 129, 320, 388, 620
MlyI GAGTC 1 cut(s) 406
MnlI CCTC 7 cut(s) 3, 175, 325, 328, 404, 453, 527
Mph1103I ATGCAT 2 cut(s) 276, 493
MseI TTAA 2 cut(s) 192, 326
MspI CCGG 1 cut(s) 454
MspR9I CCNGG 4 cut(s) 7, 349, 455, 585
MvaI CCWGG 3 cut(s) 7, 349, 585
MwoI GCNNNNNNNGC 2 cut(s) 74, 605
NciI CCSGG 1 cut(s) 455
NdeI CATATG 3 cut(s) 220, 259, 364
NlaIII CATG 2 cut(s) 274, 290
NlaIV GGNNCC 1 cut(s) 360
NsiI ATGCAT 2 cut(s) 276, 493
NspI RCATGY 2 cut(s) 274, 290
PaeI GCATGC 1 cut(s) 274
PfeI GAWTC 1 cut(s) 383
PfoI TCCNGGA 1 cut(s) 5
PkrI GCNGC 1 cut(s) 427
PleI GAGTC 1 cut(s) 405
PpsI GAGTC 1 cut(s) 405
Psp6I CCWGG 3 cut(s) 5, 347, 583
PspGI CCWGG 3 cut(s) 5, 347, 583
PspN4I GGNNCC 1 cut(s) 360
PspPI GGNCC 1 cut(s) 293
RsaI GTAC 3 cut(s) 127, 217, 515
RsaNI GTAC 3 cut(s) 126, 216, 514
SaqAI TTAA 2 cut(s) 192, 326
SatI GCNGC 1 cut(s) 426
Sau96I GGNCC 1 cut(s) 293
SchI GAGTC 1 cut(s) 406
ScrFI CCNGG 4 cut(s) 7, 349, 455, 585
SfaNI GCATC 1 cut(s) 478
SfcI CTRYAG 1 cut(s) 105
SphI GCATGC 1 cut(s) 274
Sse9I AATT 4 cut(s) 129, 320, 388, 620
SsiI CCGC 2 cut(s) 68, 156
SspMI CTAG 1 cut(s) 27
StyD4I CCNGG 4 cut(s) 5, 347, 453, 583
TaaI ACNGT 1 cut(s) 102
TaiI ACGT 1 cut(s) 305
TasI AATT 4 cut(s) 129, 320, 388, 620
TatI WGTACW 1 cut(s) 215
TfiI GAWTC 1 cut(s) 383
Tru1I TTAA 2 cut(s) 192, 326
Tru9I TTAA 2 cut(s) 192, 326
TscAI CASTG 1 cut(s) 315
TseI GCWGC 1 cut(s) 425
TspDTI ATGAA 1 cut(s) 237
TspRI CASTG 1 cut(s) 315
XapI RAATTY 1 cut(s) 620
XceI RCATGY 2 cut(s) 274, 290
XspI CTAG 1 cut(s) 27
Zsp2I ATGCAT 2 cut(s) 276, 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.