MD15G1145400.v1.1

1-aminocyclopropane-1-carboxylate synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0014996
Physical Location & Seq
Reverse (-)
10777101 .. 10779539
2439 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1145400.v1.1.491

Sequence Viewer

Length: 1383 bp
ATGTTGTCAAGGAAAGCAAGTTGTGACTCTCATGGCCAGGACTCATCCTACTTCCTGGGATGGCAGGAATACGAGAGGAACCCATATGATCAAGTTCAAAATCCAAATGGTATCATTCAGATGGGACTTGCTGAGAATCAGCTCTCGTTTGATCTTATTGAGTCATGCCTCGCAAGCAATCCCGATGCACTGGAGTTGAAAAGAAATAGAGAATCTGTTTTCAGAGAGTTGGCTCTCTTTCAAGACTACAACGGGTTCCCTGCTTTCAAGAATGAGATGGTAGAGTTCATGGCAAACATGAGAGGAAACAAAGTAAAATTTGATCCAAGCAAGCTTGTGCTCACAGCTGGTTCAACTTCTGCCAACGAAACTCTTATGTTTTGCCTTGCTGATCCCGGTGAAGCCTTCCTCCTCCCCATTCCATACTATCCAGGGTTTGATAGAGATCTGAAGTGGCGTACCGGAGTTGAAATTGTTCCAATAAAATGCACAAGTGAAAATGGATACAAAATCACTGAGTCTGCATTGCAAGAAGCCTACCAACAAGCCCAAAAATTCAACCTAAAAGTTAAAGGAGTATTGGTCACAAATCCTTCAAACCCTTTAGGCACAACAATGACTCGACTTGAACTCAACCACCTCATAGACTTCGCCATGGAAAAAGAAATACACATAATAAGCGACGAAATATACTCAGGAACCGTCTTCAACTCCCCTAGTTTCATAAGCATCGCAGAAGCTCTAACTGAAAGAAACCTAGAAGACGACGAAATACGGAATCGTTGTCACATTGTGTACAGCCTCTCTAAGGATCTCGGGCTGCCGGGTTTTCGGGTAGGCATGATTTACTCCAACAATCAAACTGTTGTGTCAGCAGCTACAAAGATGTCAAGCTTCGGACTTGTCTCTTCACAAACTCAGTACACTCTTTCCCAAATGCTGAAAGACAAGAAATTCACCGCCAACTACATGAAGGAGAATCAAAGGAGGCTTAAAAGGAGAAAGGAGATGCTTGTTTATGGGCTCAAGGGGGCAGGGATTAGGTGTTTGGAAAGCAATGCTGGGTTGTTTTGCTGGGTGGACATGAGGCACCTTTTGAGGTCCAACACTTTTGAAGCTGAAAAGGAGCTTTGGAAGAAAGTGGTATGTGAATTCGGGTTGAATATCTCTCCCGGTTCTTCTTGCCATTGCTCTGAACCGGGTTGGTTTAGAATGTGCTTTGCAAATATGTCTGAAGAAACCCTAATGGTTGCAATGCAACGTGTCAAGGCCCTCGTTGACTCCACAATTGTTCACCAGCAAACAGGACTGTCCAAATCTAGGGGGTTAAAGAGCAAGTGGGTTTTCCAATTGGCTTCATATGAGCATGAACCAGAACGTTAG

Protein Analysis

461

Amino Acids

52.29

Weight (kDa)

6.73

Isoelectric Point (pI)

42.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 39 - 421 2.6e-97 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014996)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08040
fragaria_vesca FvH4_2g32470 FvH4_3g04180
malus_domestica MD08G1076500.v1.1 MD15G1145400.v1.1
prunus_persica Prupe.1G417800_v2.0.a1
pyrus_communis pycom08g06340
rosa_chinensis RchiOBHm_Chr6g0310191
rosa_laevigata RLG00000010501
rosa_multiflora Rmu_sc0008163.1_g000012
rosa_roxburghii Rroxscaffold_7G00158760
rosa_rugosa Rorug06G0378100
rosa_samantha Rh6BG501800 Rh6CG506800 Rh6DG492900
rosa_wichuraiana Rw6G042830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1089
AciI CCGC 1 cut(s) 960
AclI AACGTT 1 cut(s) 1378
AclWI GGATC 3 cut(s) 317, 386, 819
AcoI YGGCCR 1 cut(s) 34
AcsI RAATTY 4 cut(s) 317, 554, 953, 1151
AcuI CTGAAG 2 cut(s) 470, 1254
AdeI CACNNNGTG 1 cut(s) 793
AfaI GTAC 3 cut(s) 460, 797, 923
AfiI CCNNNNNNNGG 2 cut(s) 808, 1320
AflIII ACRYGT 1 cut(s) 1261
AjnI CCWGG 3 cut(s) 36, 54, 430
AluBI AGCT 8 cut(s) 142, 334, 347, 740, 878, 894, 1118, 1129
AluI AGCT 8 cut(s) 142, 334, 347, 740, 878, 894, 1118, 1129
Alw21I GWGCWC 1 cut(s) 342
Alw26I GTCTC 1 cut(s) 910
AlwI GGATC 3 cut(s) 317, 386, 819
Ama87I CYCGRG 1 cut(s) 815
AoxI GGCC 2 cut(s) 34, 1269
ApeKI GCWGC 2 cut(s) 820, 875
ApoI RAATTY 4 cut(s) 317, 554, 953, 1151
Asp700I GAANNNNTTC 1 cut(s) 474
AspS9I GGNCC 2 cut(s) 1101, 1270
AsuC2I CCSGG 4 cut(s) 396, 825, 1173, 1200
AsuHPI GGTGA 3 cut(s) 410, 949, 1286
AvaI CYCGRG 1 cut(s) 815
AvaII GGWCC 1 cut(s) 1101
BalI TGGCCA 1 cut(s) 36
BanI GGYRCC 1 cut(s) 1089
BanII GRGCYC 1 cut(s) 1026
BbsI GAAGAC 2 cut(s) 697, 768
Bbv12I GWGCWC 1 cut(s) 342
BbvI GCAGC 2 cut(s) 807, 887
BccI CCATC 3 cut(s) 54, 115, 271
BciT130I CCWGG 3 cut(s) 38, 56, 432
BciVI GTATCC 1 cut(s) 497
BclI TGATCA 1 cut(s) 88
BcnI CCSGG 4 cut(s) 396, 825, 1173, 1200
BcoDI GTCTC 1 cut(s) 910
BfaI CTAG 3 cut(s) 717, 758, 1320
BfuI GTATCC 1 cut(s) 497
BglII AGATCT 1 cut(s) 445
BisI GCNGC 2 cut(s) 821, 876
BlsI GCNGC 2 cut(s) 822, 877
Bme1390I CCNGG 7 cut(s) 38, 56, 396, 432, 825, 1173, 1200
Bme18I GGWCC 1 cut(s) 1101
BmeT110I CYCGRG 1 cut(s) 815
BmgT120I GGNCC 2 cut(s) 1101, 1270
BmiI GGNNCC 4 cut(s) 80, 257, 700, 1091
BmrFI CCNGG 7 cut(s) 38, 56, 396, 432, 825, 1173, 1200
BmsI GCATC 3 cut(s) 175, 738, 999
BpiI GAAGAC 2 cut(s) 697, 768
BpmI CTGGAG 1 cut(s) 212
BpuEI CTTGAG 1 cut(s) 1010
BpuMI CCSGG 4 cut(s) 396, 825, 1173, 1200
BsaBI GATNNNNATC 1 cut(s) 444
BsaJI CCNNGG 3 cut(s) 55, 431, 654
BsaWI WCCGGW 1 cut(s) 461
BsaXI ACNNNNNCTCC 2 cut(s) 567, 597
Bsc4I CCNNNNNNNGG 2 cut(s) 808, 1320
Bse1I ACTGG 1 cut(s) 195
Bse3DI GCAATG 4 cut(s) 524, 1063, 1186, 1260
Bse8I GATNNNNATC 1 cut(s) 444
BseBI CCWGG 3 cut(s) 38, 56, 432
BseDI CCNNGG 3 cut(s) 55, 431, 654
BseGI GGATG 2 cut(s) 44, 65
BseJI GATNNNNATC 1 cut(s) 444
BseLI CCNNNNNNNGG 2 cut(s) 808, 1320
BseMI GCAATG 4 cut(s) 524, 1063, 1186, 1260
BseMII CTCAG 4 cut(s) 123, 507, 708, 932
BseNI ACTGG 1 cut(s) 195
BseRI GAGGAG 1 cut(s) 401
BseXI GCAGC 2 cut(s) 807, 887
BseYI CCCAGC 2 cut(s) 1061, 1074
BshFI GGCC 2 cut(s) 36, 1271
BshNI GGYRCC 1 cut(s) 1089
BsiHKAI GWGCWC 1 cut(s) 342
BsiHKCI CYCGRG 1 cut(s) 815
BsiSI CCGG 5 cut(s) 396, 462, 824, 1173, 1199
BslFI GGGAC 1 cut(s) 138
BslI CCNNNNNNNGG 2 cut(s) 808, 1320
BsmAI GTCTC 1 cut(s) 910
BsmFI GGGAC 1 cut(s) 138
BsnI GGCC 2 cut(s) 36, 1271
BsoBI CYCGRG 1 cut(s) 815
Bsp1286I GDGCHC 2 cut(s) 342, 1026
Bsp1407I TGTACA 1 cut(s) 795
Bsp143I GATC 6 cut(s) 88, 151, 322, 391, 445, 811
Bsp19I CCATGG 1 cut(s) 654
BspACI CCGC 1 cut(s) 960
BspANI GGCC 2 cut(s) 36, 1271
BspCNI CTCAG 4 cut(s) 124, 508, 707, 931
BspLI GGNNCC 4 cut(s) 80, 257, 700, 1091
BspPI GGATC 3 cut(s) 317, 386, 819
BspT107I GGYRCC 1 cut(s) 1089
BsrDI GCAATG 4 cut(s) 524, 1063, 1186, 1260
BsrGI TGTACA 1 cut(s) 795
BsrI ACTGG 1 cut(s) 195
BssECI CCNNGG 3 cut(s) 55, 431, 654
BssMI GATC 6 cut(s) 88, 151, 322, 391, 445, 811
BssT1I CCWWGG 1 cut(s) 654
Bst2UI CCWGG 3 cut(s) 38, 56, 432
Bst4CI ACNGT 3 cut(s) 703, 865, 1311
Bst6I CTCTTC 1 cut(s) 913
BstAUI TGTACA 1 cut(s) 795
BstC8I GCNNGC 2 cut(s) 175, 332
BstDEI CTNAG 5 cut(s) 132, 516, 694, 807, 918
BstDSI CCRYGG 1 cut(s) 654
BstENI CCTNNNNNAGG 1 cut(s) 806
BstF5I GGATG 2 cut(s) 44, 65
BstKTI GATC 6 cut(s) 91, 154, 325, 394, 448, 814
BstMAI GTCTC 1 cut(s) 910
BstMBI GATC 6 cut(s) 88, 151, 322, 391, 445, 811
BstMWI GCNNNNNNNGC 1 cut(s) 174
BstNI CCWGG 3 cut(s) 38, 56, 432
BstSCI CCNGG 7 cut(s) 36, 54, 394, 430, 823, 1171, 1198
BstV1I GCAGC 2 cut(s) 807, 887
BstV2I GAAGAC 2 cut(s) 697, 768
BstX2I RGATCY 2 cut(s) 445, 811
BstYI RGATCY 2 cut(s) 445, 811
BsuI GTATCC 1 cut(s) 497
BsuRI GGCC 2 cut(s) 36, 1271
BtgI CCRYGG 1 cut(s) 654
BtgZI GCGATG 1 cut(s) 715
BtsCI GGATG 2 cut(s) 44, 65
BtsIMutI CAGTG 2 cut(s) 188, 513
Cac8I GCNNGC 2 cut(s) 175, 332
Cfr13I GGNCC 2 cut(s) 1101, 1270
Csp6I GTAC 3 cut(s) 459, 796, 922
CviAII CATG 9 cut(s) 32, 165, 289, 298, 655, 841, 970, 1084, 1367
CviQI GTAC 3 cut(s) 459, 796, 922
DdeI CTNAG 5 cut(s) 132, 516, 694, 807, 918
DpnI GATC 6 cut(s) 90, 153, 324, 393, 447, 813
DpnII GATC 6 cut(s) 88, 151, 322, 391, 445, 811
DraIII CACNNNGTG 1 cut(s) 793
EaeI YGGCCR 1 cut(s) 34
Eam1104I CTCTTC 1 cut(s) 913
EarI CTCTTC 1 cut(s) 913
Eco130I CCWWGG 1 cut(s) 654
Eco24I GRGCYC 1 cut(s) 1026
Eco47I GGWCC 1 cut(s) 1101
Eco57I CTGAAG 2 cut(s) 470, 1254
Eco88I CYCGRG 1 cut(s) 815
EcoNI CCTNNNNNAGG 1 cut(s) 806
EcoO109I RGGNCCY 1 cut(s) 1270
EcoRI GAATTC 1 cut(s) 1151
EcoRII CCWGG 3 cut(s) 36, 54, 430
EcoT14I CCWWGG 1 cut(s) 654
EcoT38I GRGCYC 1 cut(s) 1026
ErhI CCWWGG 1 cut(s) 654
FaeI CATG 9 cut(s) 35, 168, 292, 301, 658, 844, 973, 1087, 1370
FaqI GGGAC 1 cut(s) 138
FatI CATG 9 cut(s) 31, 164, 288, 297, 654, 840, 969, 1083, 1366
FauNDI CATATG 2 cut(s) 85, 1360
FbaI TGATCA 1 cut(s) 88
Fnu4HI GCNGC 2 cut(s) 821, 876
FokI GGATG 2 cut(s) 31, 72
FriOI GRGCYC 1 cut(s) 1026
Fsp4HI GCNGC 2 cut(s) 821, 876
FspBI CTAG 3 cut(s) 717, 758, 1320
GluI GCNGC 2 cut(s) 821, 876
GsaI CCCAGC 2 cut(s) 1065, 1078
GsuI CTGGAG 1 cut(s) 212
HaeIII GGCC 2 cut(s) 36, 1271
HapII CCGG 5 cut(s) 396, 462, 824, 1173, 1199
Hin1II CATG 9 cut(s) 35, 168, 292, 301, 658, 844, 973, 1087, 1370
HincII GTYRAC 1 cut(s) 1279
HindII GTYRAC 1 cut(s) 1279
HindIII AAGCTT 2 cut(s) 332, 892
HpaII CCGG 5 cut(s) 396, 462, 824, 1173, 1199
HphI GGTGA 3 cut(s) 410, 949, 1286
Hpy166II GTNNAC 5 cut(s) 796, 924, 1081, 1279, 1294
Hpy188I TCNGA 6 cut(s) 120, 224, 450, 899, 1195, 1234
Hpy188III TCNNGA 4 cut(s) 182, 242, 268, 696
Hpy8I GTNNAC 5 cut(s) 796, 924, 1081, 1279, 1294
Hpy99I CGWCG 2 cut(s) 686, 770
HpyAV CCTTC 3 cut(s) 415, 603, 967
HpyCH4III ACNGT 3 cut(s) 703, 865, 1311
HpyCH4IV ACGT 2 cut(s) 1261, 1378
HpyCH4V TGCA 7 cut(s) 188, 489, 524, 529, 1223, 1253, 1258
HpyF10VI GCNNNNNNNGC 1 cut(s) 174
HpyF3I CTNAG 5 cut(s) 132, 516, 694, 807, 918
HpySE526I ACGT 2 cut(s) 1261, 1378
Hsp92II CATG 9 cut(s) 35, 168, 292, 301, 658, 844, 973, 1087, 1370
Ksp22I TGATCA 1 cut(s) 88
Kzo9I GATC 6 cut(s) 88, 151, 322, 391, 445, 811
LmnI GCTCC 1 cut(s) 1126
Lsp1109I GCAGC 2 cut(s) 807, 887
LweI GCATC 3 cut(s) 175, 738, 999
MaeI CTAG 3 cut(s) 717, 758, 1320
MaeII ACGT 2 cut(s) 1261, 1378
MaeIII GTNAC 3 cut(s) 23, 583, 785
MalI GATC 6 cut(s) 90, 153, 324, 393, 447, 813
MboI GATC 6 cut(s) 88, 151, 322, 391, 445, 811
MboII GAAGA 6 cut(s) 697, 773, 900, 1147, 1170, 1247
MfeI CAATTG 2 cut(s) 1287, 1349
MflI RGATCY 2 cut(s) 445, 811
MhlI GDGCHC 2 cut(s) 342, 1026
MlsI TGGCCA 1 cut(s) 36
MluCI AATT 7 cut(s) 317, 471, 554, 953, 1151, 1287, 1349
MluNI TGGCCA 1 cut(s) 36
MlyI GAGTC 6 cut(s) 20, 35, 170, 527, 613, 1274
MmeI TCCRAC 2 cut(s) 876, 1128
Mox20I TGGCCA 1 cut(s) 36
MroXI GAANNNNTTC 1 cut(s) 474
MscI TGGCCA 1 cut(s) 36
MseI TTAA 3 cut(s) 570, 993, 1328
MslI CAYNNNNRTG 2 cut(s) 119, 614
Msp20I TGGCCA 1 cut(s) 36
MspA1I CMGCKG 1 cut(s) 347
MspI CCGG 5 cut(s) 396, 462, 824, 1173, 1199
MspR9I CCNGG 7 cut(s) 38, 56, 396, 432, 825, 1173, 1200
MunI CAATTG 2 cut(s) 1287, 1349
MvaI CCWGG 3 cut(s) 38, 56, 432
MwoI GCNNNNNNNGC 1 cut(s) 174
NciI CCSGG 4 cut(s) 396, 825, 1173, 1200
NcoI CCATGG 1 cut(s) 654
NdeI CATATG 2 cut(s) 85, 1360
NdeII GATC 6 cut(s) 88, 151, 322, 391, 445, 811
NlaIII CATG 9 cut(s) 35, 168, 292, 301, 658, 844, 973, 1087, 1370
NlaIV GGNNCC 4 cut(s) 80, 257, 700, 1091
NmuCI GTSAC 3 cut(s) 23, 583, 785
PdmI GAANNNNTTC 1 cut(s) 474
PfeI GAWTC 4 cut(s) 136, 212, 778, 979
PkrI GCNGC 2 cut(s) 822, 877
PleI GAGTC 6 cut(s) 20, 35, 169, 526, 613, 1274
PpsI GAGTC 6 cut(s) 20, 35, 169, 526, 613, 1274
Psp1406I AACGTT 1 cut(s) 1378
Psp6I CCWGG 3 cut(s) 36, 54, 430
PspFI CCCAGC 2 cut(s) 1061, 1074
PspGI CCWGG 3 cut(s) 36, 54, 430
PspN4I GGNNCC 4 cut(s) 80, 257, 700, 1091
PspPI GGNCC 2 cut(s) 1101, 1270
PsuI RGATCY 2 cut(s) 445, 811
PvuII CAGCTG 1 cut(s) 347
RsaI GTAC 3 cut(s) 460, 797, 923
RsaNI GTAC 3 cut(s) 459, 796, 922
RseI CAYNNNNRTG 2 cut(s) 119, 614
SaqAI TTAA 3 cut(s) 570, 993, 1328
SatI GCNGC 2 cut(s) 821, 876
Sau3AI GATC 6 cut(s) 88, 151, 322, 391, 445, 811
Sau96I GGNCC 2 cut(s) 1101, 1270
SchI GAGTC 6 cut(s) 20, 35, 170, 527, 613, 1274
ScrFI CCNGG 7 cut(s) 38, 56, 396, 432, 825, 1173, 1200
SduI GDGCHC 2 cut(s) 342, 1026
SfaNI GCATC 3 cut(s) 175, 738, 999
SinI GGWCC 1 cut(s) 1101
SmiMI CAYNNNNRTG 2 cut(s) 119, 614
SmlI CTYRAG 1 cut(s) 1025
SmoI CTYRAG 1 cut(s) 1025
Sse9I AATT 7 cut(s) 317, 471, 554, 953, 1151, 1287, 1349
SsiI CCGC 1 cut(s) 960
SspMI CTAG 3 cut(s) 717, 758, 1320
StyD4I CCNGG 7 cut(s) 36, 54, 394, 430, 823, 1171, 1198
StyI CCWWGG 1 cut(s) 654
TaaI ACNGT 3 cut(s) 703, 865, 1311
TaiI ACGT 2 cut(s) 1264, 1381
TaqI TCGA 1 cut(s) 622
TasI AATT 7 cut(s) 317, 471, 554, 953, 1151, 1287, 1349
TatI WGTACW 2 cut(s) 795, 921
TfiI GAWTC 4 cut(s) 136, 212, 778, 979
Tru1I TTAA 3 cut(s) 570, 993, 1328
Tru9I TTAA 3 cut(s) 570, 993, 1328
TscAI CASTG 2 cut(s) 195, 520
TseFI GTSAC 3 cut(s) 23, 583, 785
TseI GCWGC 2 cut(s) 820, 875
Tsp45I GTSAC 3 cut(s) 23, 583, 785
TspDTI ATGAA 5 cut(s) 277, 712, 986, 1347, 1383
TspGWI ACGGA 1 cut(s) 790
TspRI CASTG 2 cut(s) 195, 520
VpaK11BI GGWCC 1 cut(s) 1101
XagI CCTNNNNNAGG 1 cut(s) 806
XapI RAATTY 4 cut(s) 317, 554, 953, 1151
XmnI GAANNNNTTC 1 cut(s) 474
XspI CTAG 3 cut(s) 717, 758, 1320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.