AT4G09360

Toll - interleukin 1 - resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0016584
Physical Location & Seq
Forward (+)
5940186 .. 5943280
3095 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G09360.1

Sequence Viewer

Length: 2562 bp
ATGGACGTTGACATGGAACGACTGAATCCTCTATTGAGCATCGAATCTGAGAATGAGGTTCGTATGATCGGAATCTGGGGAATGGGAGGCATAGGCAAAACCACCATTGCCAAGTGTCTCTATGAAGAATATTCGCGCCGATTTGTTCACTATTGTTTCATAGAAAACGTTAGGATTTTTGCAAAGAATGGCCTCCCATATTTGCAGGAAAAGCTTCTATCTGAAATCCGAGGCAAAAAGCAAGAGACCTTATGGAGCGTGGAGAAGGGATGCAGACTTATCAAGTCGAAGCTCAAGGAAAAAAATTTCCTTGTCCTTGACGATGTGGACAATGTGGACCAGCTGCATGCCCTAGCAAAAGAGACGAGCTGGTTTGGACCAGGGAGCCGAATCATCATAACTACACGAGACTTTGGCTTACTCTACTCCTTTGGAGTGAGATTATTATACCGTGTTAGCTTTTTGGATGACAATGATGCTATCAAAGTCTTTAAACATGTTGCTTTTGACGGAGGGCAACCTCCTTTTGATGTCTACCATCAATTCTCAGTCCGTGCTTCTAGGCTTGCTCAAGGCCTTCCTTCAGCCCTTGAGGCTTTTGGCGCGTTGGGCATTCTTGAAACAGTTCCTCAAAAACGTATCAAGGATATTTTAAAAACTAGCTATGACGGTTTAGACGAGGAAGAGCAAGCTGCGTTCCTTCATGTTGCATGTCTATTCAATGGAGACTCTGTCCACCGGGTAAATGCCCTTATCGATGATGGTGACATGAGGATAAAAGCTTTAGAAGTGAAGTCTCTCATAGACATATCACTTGATGGATGCATTACATTGCATGTCTTGATAGAACAAGCGGCAAGAGAAATTGATACAGCAATGGTTGAAGGTGTAGCACTTCACATGTGTGAGATGATACACGTGTTGCCTATCGACGGAAATATTCTTAACACTATCAACAATCTCAAATTCTTCAAGGCTTTCACGCATATAGAGGTTATGAATTCCAAATTGCAGTTTCTTCCAGGCACAGCTAACAATTCAGGGGGCCTTGGTCAGTTGAGGAGACTAGATGTTACTGGCTCTAAGAATCTGAGAGAAATTCCAGATCTCTCGAGGACCATGCTGCTCGAGGAGTTGATAATGAAAGGCTGCACAAGATTGGAGAAAACTCTAGATATCAGAAAAGGCTGTCCTTCCAGAACGTGGACTAAGGCGACGCCGACAAATAATATTGAGGCTTCCCAGAGGAGTAAAGAAGCTGAGTTCTCTTGCAAACCTATCTATAGAAGGGAAGATACACATCGGCTTGTGGCATCTCACGGGAACCGTAGAGCACCTCTCCTTTATTCCGAGCAAAAAATCCCTGATGAATTGGTAATGGTGCCAAAGAAAAGATTTCCGATCATCTCAAGCTTTTATGATCTTAAATCTCTCAGCATCATGCGGTTTAGCCACATTGCAGATGGTACCCCCTTCCGTTGCATTAGCTTCTCAGGTTTCCAGTGCTTGGTAGAGTTGAATCTGATCAACTTAAACATCCAGAAAATCCCAGACAACATTGGTCTAATGCAGTCACTAGAGAAAGTGGACCTCAGCGGAAATGATTTCAGGAATCTACCCGCATCAACAAAGAACCTTTCGAAGTTGAAATACGCGAGGCTCTCTAATTGCATCAAACTCGAGGCATTTGTAGAGCTGACTGAGTTGCAGACGCTCAAGCTTTCTGGTTGTACGAACCTCGAATCTCTGCTGGAACTTCCTTATGCGGTTCAAGATGTAGGCAGATTCTGTTTGCTAGCGCTTGAGCTTGATAACTGCAAGAATCTCCAAGCATTATCAGAACAGCTAAGCCATTTCAGCAATTTAATACACTTGGACCTAAGCAGCCACGACTTTGAGAAACTCAAATCAGTGGAAGAGCTTCCACTAAACCTCAAGCATCTCTACGCACACGGCTGTGATTCCCTGGAAAGTGTCGACCTTTCCCCAAAGCATTCAATCAAACACCTCGATCTTAGCCATTGCTTCGGTTTGCAACAAGATGAGCAACAGATTACACAATTTCTAAACGACAAGTGTAGCCAAGAGGTTTCACAAAGATTTCTTTGCTTACCAGGAACTGAAGTACCCAGAAATTTCGATAATCAGTCACATGGGACATCGACGAAGATCAGCTTGTTTACACCGACACTTCTGAGTTTCGCTGCCTGTATCTTGATTTCTTGCGAGAGGTCCTTCTACCTCCAATTCCCAGCGTTTAGTTATGATTGGAACCGCAAAGATGATGAAGTAATTTCGATTAACCTCACACCAAATCTCAATCTTTCATCGGAGATTGAGGAAGAAGAGACTGTAACATCACATCACCTGGTTATCATCCACGTACCCAGTAGCATAAACACCGACAAAATCGAAGATTTGCGACTCGAATCCTCTCTTCAATTTCCAGAGGAGTTTCAGTTTTCACCCTGCGAGATAAGAGCTTGTGGAATTCGCATGGTTGACGAAGAGACTAAATGTCTGGAGAGCTCACCTCTTATGCCACTATTGGAGAAGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

853

Amino Acids

96.66

Weight (kDa)

5.78

Isoelectric Point (pI)

47.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 5 - 170 4.4e-15 NB-ARC domain
WHD_ROQ1 PF23282 227 - 289 5.5e-06 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 506 - 657 4.4e-10 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1466
AccB1I GGYRCC 2 cut(s) 1381, 1466
AccB7I CCANNNNNTGG 2 cut(s) 1203, 1507
AccI GTMKAC 2 cut(s) 534, 1977
AccII CGCG 3 cut(s) 136, 605, 1655
AciI CCGC 6 cut(s) 854, 1444, 1596, 1620, 1766, 2275
AclI AACGTT 1 cut(s) 168
AcsI RAATTY 6 cut(s) 304, 965, 1000, 1098, 2134, 2490
AcuI CTGAAG 2 cut(s) 567, 2142
AcvI CACGTG 1 cut(s) 919
AcyI GRCGYC 1 cut(s) 1217
AfaI GTAC 4 cut(s) 1468, 1732, 2127, 2385
AfeI AGCGCT 1 cut(s) 1800
AfiI CCNNNNNNNGG 3 cut(s) 932, 1203, 1507
AflIII ACRYGT 4 cut(s) 496, 900, 916, 918
AgsI TTSAA 9 cut(s) 620, 721, 884, 973, 1519, 1648, 1772, 1998, 2441
AhdI GACNNNNNGTC 1 cut(s) 2517
AjnI CCWGG 5 cut(s) 379, 1021, 1965, 2113, 2367
AleI CACNNNNGTG 2 cut(s) 903, 1956
Alw21I GWGCWC 2 cut(s) 1336, 2531
Alw26I GTCTC 9 cut(s) 122, 239, 356, 402, 720, 801, 1057, 2342, 2504
AlwNI CAGNNNCTG 2 cut(s) 1788, 2120
Ama87I CYCGRG 3 cut(s) 1111, 1127, 1679
Aor51HI AGCGCT 1 cut(s) 1800
AoxI GGCC 3 cut(s) 190, 574, 1045
ApeKI GCWGC 6 cut(s) 343, 692, 1123, 1149, 1884, 2204
ApoI RAATTY 6 cut(s) 304, 965, 1000, 1098, 2134, 2490
Asp700I GAANNNNTTC 3 cut(s) 213, 624, 1920
Asp718I GGTACC 1 cut(s) 1466
AspLEI GCGC 3 cut(s) 138, 605, 1801
AspS9I GGNCC 7 cut(s) 337, 377, 1045, 1116, 1588, 1876, 2232
AsuC2I CCSGG 1 cut(s) 740
AsuHPI GGTGA 4 cut(s) 776, 2357, 2457, 2523
AsuII TTCGAA 1 cut(s) 1640
AsuNHI GCTAGC 1 cut(s) 1795
AvaI CYCGRG 3 cut(s) 1111, 1127, 1679
AvaII GGWCC 6 cut(s) 337, 377, 1116, 1588, 1876, 2232
BaeI ACNNNNGTAYC 4 cut(s) 905, 938, 2109, 2142
BanI GGYRCC 2 cut(s) 1381, 1466
BanII GRGCYC 1 cut(s) 2531
BarI GAAGNNNNNNTAC 4 cut(s) 1279, 1311, 1634, 1666
BauI CACGAG 1 cut(s) 405
BbrPI CACGTG 1 cut(s) 919
Bbv12I GWGCWC 2 cut(s) 1336, 2531
BbvCI CCTCAGC 1 cut(s) 1592
BbvI GCAGC 6 cut(s) 330, 679, 1110, 1136, 1896, 2191
BccI CCATC 4 cut(s) 546, 755, 812, 1457
BceAI ACGGC 1 cut(s) 1969
BcgI CGANNNNNNTGC 4 cut(s) 1102, 1136, 1660, 1694
BciT130I CCWGG 5 cut(s) 381, 1023, 1967, 2115, 2369
BclI TGATCA 1 cut(s) 1524
BcnI CCSGG 1 cut(s) 740
BcoDI GTCTC 9 cut(s) 122, 239, 356, 402, 720, 801, 1057, 2342, 2504
BfaI CTAG 7 cut(s) 353, 561, 660, 1067, 1172, 1577, 1796
BfmI CTRYAG 1 cut(s) 1282
BfoI RGCGCY 1 cut(s) 1802
BglI GCCNNNNNGGC 1 cut(s) 593
BglII AGATCT 1 cut(s) 1105
BisI GCNGC 7 cut(s) 344, 693, 855, 1124, 1150, 1885, 2205
BlpI GCTNAGC 1 cut(s) 1847
BlsI GCNGC 7 cut(s) 345, 694, 856, 1125, 1151, 1886, 2206
Bme1390I CCNGG 6 cut(s) 381, 740, 1023, 1967, 2115, 2369
Bme18I GGWCC 6 cut(s) 337, 377, 1116, 1588, 1876, 2232
BmeRI GACNNNNNGTC 1 cut(s) 2517
BmeT110I CYCGRG 3 cut(s) 1111, 1127, 1679
BmgT120I GGNCC 7 cut(s) 337, 377, 1045, 1116, 1588, 1876, 2232
BmiI GGNNCC 6 cut(s) 386, 1046, 1325, 1383, 1468, 2273
BmrFI CCNGG 6 cut(s) 381, 740, 1023, 1967, 2115, 2369
BmrI ACTGGG 1 cut(s) 2382
BmsI GCATC 9 cut(s) 48, 260, 466, 812, 1322, 1446, 1631, 1680, 1948
BmtI GCTAGC 1 cut(s) 1799
BmuI ACTGGG 1 cut(s) 2382
BplI GAGNNNNNCTC 4 cut(s) 1323, 1355, 2518, 2550
BpmI CTGGAG 1 cut(s) 2543
Bpu10I CCTNAGC 2 cut(s) 1592, 1880
Bpu1102I GCTNAGC 1 cut(s) 1847
Bpu14I TTCGAA 1 cut(s) 1640
BpuEI CTTGAG 7 cut(s) 278, 555, 611, 1393, 1700, 1823, 1919
BpuMI CCSGG 1 cut(s) 740
Bsa29I ATCGAT 1 cut(s) 756
BsaAI YACGTR 2 cut(s) 919, 2383
BsaBI GATNNNNATC 2 cut(s) 71, 1299
BsaHI GRCGYC 1 cut(s) 1217
BsaI GGTCTC 1 cut(s) 239
BsaJI CCNNGG 4 cut(s) 229, 380, 1048, 1965
BsaXI ACNNNNNCTCC 4 cut(s) 717, 747, 2444, 2474
Bsc4I CCNNNNNNNGG 3 cut(s) 932, 1203, 1507
Bse1I ACTGG 3 cut(s) 1081, 1501, 2388
Bse3DI GCAATG 5 cut(s) 105, 830, 882, 1455, 2020
Bse8I GATNNNNATC 2 cut(s) 71, 1299
BseBI CCWGG 5 cut(s) 381, 1023, 1967, 2115, 2369
BseCI ATCGAT 1 cut(s) 756
BseDI CCNNGG 4 cut(s) 229, 380, 1048, 1965
BseGI GGATG 5 cut(s) 275, 472, 827, 1536, 2376
BseJI GATNNNNATC 2 cut(s) 71, 1299
BseLI CCNNNNNNNGG 3 cut(s) 932, 1203, 1507
BseMI GCAATG 5 cut(s) 105, 830, 882, 1455, 2020
BseMII CTCAG 9 cut(s) 39, 561, 1082, 1251, 1447, 1506, 1606, 1692, 2186
BseNI ACTGG 3 cut(s) 1081, 1501, 2388
BseRI GAGGAG 4 cut(s) 1075, 1145, 1261, 2465
BseXI GCAGC 6 cut(s) 330, 679, 1110, 1136, 1896, 2191
BseYI CCCAGC 1 cut(s) 2251
BsgI GTGCAG 1 cut(s) 1135
Bsh1236I CGCG 3 cut(s) 136, 605, 1655
BshFI GGCC 3 cut(s) 192, 576, 1047
BshNI GGYRCC 2 cut(s) 1381, 1466
BshVI ATCGAT 1 cut(s) 756
BsiHKAI GWGCWC 2 cut(s) 1336, 2531
BsiHKCI CYCGRG 3 cut(s) 1111, 1127, 1679
BsiSI CCGG 1 cut(s) 739
BslFI GGGAC 1 cut(s) 2170
BslI CCNNNNNNNGG 3 cut(s) 932, 1203, 1507
BsmAI GTCTC 9 cut(s) 122, 239, 356, 402, 720, 801, 1057, 2342, 2504
BsmBI CGTCTC 1 cut(s) 356
BsmFI GGGAC 1 cut(s) 2170
BsmI GAATGC 2 cut(s) 612, 1993
BsnI GGCC 3 cut(s) 192, 576, 1047
Bso31I GGTCTC 1 cut(s) 239
BsoBI CYCGRG 3 cut(s) 1111, 1127, 1679
Bsp119I TTCGAA 1 cut(s) 1640
Bsp1286I GDGCHC 2 cut(s) 1336, 2531
Bsp143I GATC 7 cut(s) 66, 1105, 1401, 1420, 1524, 2011, 2169
Bsp1720I GCTNAGC 1 cut(s) 1847
BspACI CCGC 6 cut(s) 854, 1444, 1596, 1620, 1766, 2275
BspANI GGCC 3 cut(s) 192, 576, 1047
BspCNI CTCAG 9 cut(s) 40, 560, 1083, 1252, 1446, 1505, 1605, 1693, 2187
BspDI ATCGAT 1 cut(s) 756
BspFNI CGCG 3 cut(s) 136, 605, 1655
BspLI GGNNCC 6 cut(s) 386, 1046, 1325, 1383, 1468, 2273
BspOI GCTAGC 1 cut(s) 1799
BspQI GCTCTTC 2 cut(s) 678, 1911
BspT104I TTCGAA 1 cut(s) 1640
BspT107I GGYRCC 2 cut(s) 1381, 1466
BspTNI GGTCTC 1 cut(s) 239
BsrDI GCAATG 5 cut(s) 105, 830, 882, 1455, 2020
BsrI ACTGG 3 cut(s) 1081, 1501, 2388
BssECI CCNNGG 4 cut(s) 229, 380, 1048, 1965
BssMI GATC 7 cut(s) 66, 1105, 1401, 1420, 1524, 2011, 2169
BssNI GRCGYC 1 cut(s) 1217
BssSI CACGAG 1 cut(s) 405
BssT1I CCWWGG 1 cut(s) 1048
Bst2BI CACGAG 1 cut(s) 405
Bst2UI CCWGG 5 cut(s) 381, 1023, 1967, 2115, 2369
Bst4CI ACNGT 5 cut(s) 452, 625, 671, 1328, 2353
Bst6I CTCTTC 5 cut(s) 678, 1911, 2340, 2442, 2502
BstACI GRCGYC 1 cut(s) 1217
BstBAI YACGTR 2 cut(s) 919, 2383
BstBI TTCGAA 1 cut(s) 1640
BstC8I GCNNGC 4 cut(s) 348, 567, 690, 1797
BstF5I GGATG 5 cut(s) 275, 472, 827, 1536, 2376
BstFNI CGCG 3 cut(s) 136, 605, 1655
BstH2I RGCGCY 1 cut(s) 1802
BstHHI GCGC 3 cut(s) 138, 605, 1801
BstKTI GATC 7 cut(s) 69, 1108, 1404, 1423, 1527, 2014, 2172
BstMAI GTCTC 9 cut(s) 122, 239, 356, 402, 720, 801, 1057, 2342, 2504
BstMBI GATC 7 cut(s) 66, 1105, 1401, 1420, 1524, 2011, 2169
BstMWI GCNNNNNNNGC 5 cut(s) 211, 593, 602, 609, 1857
BstNI CCWGG 5 cut(s) 381, 1023, 1967, 2115, 2369
BstNSI RCATGY 5 cut(s) 350, 500, 714, 839, 904
BstSCI CCNGG 6 cut(s) 379, 738, 1021, 1965, 2113, 2367
BstSFI CTRYAG 1 cut(s) 1282
BstUI CGCG 3 cut(s) 136, 605, 1655
BstV1I GCAGC 6 cut(s) 330, 679, 1110, 1136, 1896, 2191
BstX2I RGATCY 1 cut(s) 1105
BstYI RGATCY 1 cut(s) 1105
Bsu15I ATCGAT 1 cut(s) 756
BsuRI GGCC 3 cut(s) 192, 576, 1047
BsuTUI ATCGAT 1 cut(s) 756
BtsCI GGATG 5 cut(s) 275, 472, 827, 1536, 2376
BtsIMutI CAGTG 2 cut(s) 1508, 1917
Cac8I GCNNGC 4 cut(s) 348, 567, 690, 1797
CaiI CAGNNNCTG 2 cut(s) 1788, 2120
CfoI GCGC 3 cut(s) 138, 605, 1801
Cfr13I GGNCC 7 cut(s) 337, 377, 1045, 1116, 1588, 1876, 2232
ClaI ATCGAT 1 cut(s) 756
CseI GACGC 2 cut(s) 1225, 1720
CsiI ACCWGGT 1 cut(s) 2367
Csp6I GTAC 4 cut(s) 1467, 1731, 2126, 2384
CviQI GTAC 4 cut(s) 1467, 1731, 2126, 2384
DpnI GATC 7 cut(s) 68, 1107, 1403, 1422, 1526, 2013, 2171
DpnII GATC 7 cut(s) 66, 1105, 1401, 1420, 1524, 2011, 2169
DraI TTTAAA 2 cut(s) 493, 654
DriI GACNNNNNGTC 1 cut(s) 2517
Eam1104I CTCTTC 5 cut(s) 678, 1911, 2340, 2442, 2502
Eam1105I GACNNNNNGTC 1 cut(s) 2517
EarI CTCTTC 5 cut(s) 678, 1911, 2340, 2442, 2502
Ecl136II GAGCTC 1 cut(s) 2529
Eco130I CCWWGG 1 cut(s) 1048
Eco147I AGGCCT 1 cut(s) 576
Eco24I GRGCYC 1 cut(s) 2531
Eco31I GGTCTC 1 cut(s) 239
Eco32I GATATC 1 cut(s) 1177
Eco47I GGWCC 6 cut(s) 337, 377, 1116, 1588, 1876, 2232
Eco47III AGCGCT 1 cut(s) 1800
Eco53kI GAGCTC 1 cut(s) 2529
Eco57I CTGAAG 2 cut(s) 567, 2142
Eco72I CACGTG 1 cut(s) 919
Eco88I CYCGRG 3 cut(s) 1111, 1127, 1679
EcoICRI GAGCTC 1 cut(s) 2529
EcoO109I RGGNCCY 2 cut(s) 1045, 2232
EcoRI GAATTC 2 cut(s) 1000, 2490
EcoRII CCWGG 5 cut(s) 379, 1021, 1965, 2113, 2367
EcoRV GATATC 1 cut(s) 1177
EcoT14I CCWWGG 1 cut(s) 1048
EcoT22I ATGCAT 1 cut(s) 827
EcoT38I GRGCYC 1 cut(s) 2531
ErhI CCWWGG 1 cut(s) 1048
Esp3I CGTCTC 1 cut(s) 356
FalI AAGNNNNNCTT 2 cut(s) 2159, 2191
FaqI GGGAC 1 cut(s) 2170
FauI CCCGC 1 cut(s) 1627
FbaI TGATCA 1 cut(s) 1524
FblI GTMKAC 2 cut(s) 534, 1977
Fnu4HI GCNGC 7 cut(s) 344, 693, 855, 1124, 1150, 1885, 2205
FokI GGATG 5 cut(s) 282, 479, 834, 1523, 2363
FriOI GRGCYC 1 cut(s) 2531
Fsp4HI GCNGC 7 cut(s) 344, 693, 855, 1124, 1150, 1885, 2205
FspBI CTAG 7 cut(s) 353, 561, 660, 1067, 1172, 1577, 1796
GlaI GCGC 3 cut(s) 137, 604, 1800
GluI GCNGC 7 cut(s) 344, 693, 855, 1124, 1150, 1885, 2205
GsaI CCCAGC 1 cut(s) 2255
GsuI CTGGAG 1 cut(s) 2543
HaeII RGCGCY 1 cut(s) 1802
HaeIII GGCC 3 cut(s) 192, 576, 1047
HapII CCGG 1 cut(s) 739
HgaI GACGC 2 cut(s) 1225, 1720
HhaI GCGC 3 cut(s) 138, 605, 1801
Hin1I GRCGYC 1 cut(s) 1217
Hin6I GCGC 3 cut(s) 136, 603, 1799
HinP1I GCGC 3 cut(s) 136, 603, 1799
HincII GTYRAC 3 cut(s) 10, 1978, 2503
HindII GTYRAC 3 cut(s) 10, 1978, 2503
HindIII AAGCTT 4 cut(s) 212, 780, 1411, 1718
HpaII CCGG 1 cut(s) 739
HphI GGTGA 4 cut(s) 776, 2357, 2457, 2523
Hpy99I CGWCG 3 cut(s) 935, 1219, 2167
HpyAV CCTTC 9 cut(s) 259, 587, 591, 710, 878, 1203, 1281, 1483, 2245
HpyCH4III ACNGT 5 cut(s) 452, 625, 671, 1328, 2353
HpyCH4IV ACGT 6 cut(s) 6, 168, 637, 918, 1202, 2382
HpyF10VI GCNNNNNNNGC 5 cut(s) 211, 593, 602, 609, 1857
HpySE526I ACGT 6 cut(s) 6, 168, 637, 918, 1202, 2382
Hsp92I GRCGYC 1 cut(s) 1217
HspAI GCGC 3 cut(s) 136, 603, 1799
KpnI GGTACC 1 cut(s) 1470
Ksp22I TGATCA 1 cut(s) 1524
Kzo9I GATC 7 cut(s) 66, 1105, 1401, 1420, 1524, 2011, 2169
LguI GCTCTTC 2 cut(s) 678, 1911
LmnI GCTCC 2 cut(s) 255, 384
Lsp1109I GCAGC 6 cut(s) 330, 679, 1110, 1136, 1896, 2191
LweI GCATC 9 cut(s) 48, 260, 466, 812, 1322, 1446, 1631, 1680, 1948
MabI ACCWGGT 1 cut(s) 2367
MaeI CTAG 7 cut(s) 353, 561, 660, 1067, 1172, 1577, 1796
MaeII ACGT 6 cut(s) 6, 168, 637, 918, 1202, 2382
MaeIII GTNAC 5 cut(s) 764, 1072, 1572, 2148, 2353
MalI GATC 7 cut(s) 68, 1107, 1403, 1422, 1526, 2013, 2171
MboI GATC 7 cut(s) 66, 1105, 1401, 1420, 1524, 2011, 2169
MflI RGATCY 1 cut(s) 1105
MhlI GDGCHC 2 cut(s) 1336, 2531
MlyI GAGTC 2 cut(s) 722, 2418
Mph1103I ATGCAT 1 cut(s) 827
MroXI GAANNNNTTC 3 cut(s) 213, 624, 1920
MseI TTAA 7 cut(s) 492, 653, 945, 1425, 1532, 1865, 2301
MslI CAYNNNNRTG 2 cut(s) 903, 1956
MspA1I CMGCKG 2 cut(s) 343, 1596
MspI CCGG 1 cut(s) 739
MspR9I CCNGG 6 cut(s) 381, 740, 1023, 1967, 2115, 2369
Mva1269I GAATGC 2 cut(s) 612, 1993
MvaI CCWGG 5 cut(s) 381, 1023, 1967, 2115, 2369
MvnI CGCG 3 cut(s) 136, 605, 1655
MwoI GCNNNNNNNGC 5 cut(s) 211, 593, 602, 609, 1857
NciI CCSGG 1 cut(s) 740
NdeII GATC 7 cut(s) 66, 1105, 1401, 1420, 1524, 2011, 2169
NheI GCTAGC 1 cut(s) 1795
NlaIV GGNNCC 6 cut(s) 386, 1046, 1325, 1383, 1468, 2273
NmuCI GTSAC 3 cut(s) 764, 1572, 2148
NsiI ATGCAT 1 cut(s) 827
NspI RCATGY 5 cut(s) 350, 500, 714, 839, 904
NspV TTCGAA 1 cut(s) 1640
OliI CACNNNNGTG 2 cut(s) 903, 1956
PaeI GCATGC 1 cut(s) 350
PaeR7I CTCGAG 3 cut(s) 1111, 1127, 1679
PceI AGGCCT 1 cut(s) 576
PciI ACATGT 2 cut(s) 496, 900
PciSI GCTCTTC 2 cut(s) 678, 1911
PcsI WCGNNNNNNNCGW 1 cut(s) 675
PctI GAATGC 2 cut(s) 612, 1993
PdmI GAANNNNTTC 3 cut(s) 213, 624, 1920
PflFI GACNNNGTC 1 cut(s) 731
PflMI CCANNNNNTGG 2 cut(s) 1203, 1507
PkrI GCNGC 7 cut(s) 345, 694, 856, 1125, 1151, 1886, 2206
PleI GAGTC 2 cut(s) 722, 2418
PmaCI CACGTG 1 cut(s) 919
PmlI CACGTG 1 cut(s) 919
PpsI GAGTC 2 cut(s) 722, 2418
Ppu21I YACGTR 2 cut(s) 919, 2383
PpuMI RGGWCCY 1 cut(s) 2232
PscI ACATGT 2 cut(s) 496, 900
Psp124BI GAGCTC 1 cut(s) 2531
Psp1406I AACGTT 1 cut(s) 168
Psp5II RGGWCCY 1 cut(s) 2232
Psp6I CCWGG 5 cut(s) 379, 1021, 1965, 2113, 2367
PspCI CACGTG 1 cut(s) 919
PspFI CCCAGC 1 cut(s) 2251
PspGI CCWGG 5 cut(s) 379, 1021, 1965, 2113, 2367
PspN4I GGNNCC 6 cut(s) 386, 1046, 1325, 1383, 1468, 2273
PspPI GGNCC 7 cut(s) 337, 377, 1045, 1116, 1588, 1876, 2232
PspPPI RGGWCCY 1 cut(s) 2232
PspXI VCTCGAGB 2 cut(s) 1127, 1679
PstNI CAGNNNCTG 2 cut(s) 1788, 2120
PsuI RGATCY 1 cut(s) 1105
PsyI GACNNNGTC 1 cut(s) 731
PvuII CAGCTG 1 cut(s) 343
RsaI GTAC 4 cut(s) 1468, 1732, 2127, 2385
RsaNI GTAC 4 cut(s) 1467, 1731, 2126, 2384
RseI CAYNNNNRTG 2 cut(s) 903, 1956
SacI GAGCTC 1 cut(s) 2531
SalI GTCGAC 1 cut(s) 1976
SapI GCTCTTC 2 cut(s) 678, 1911
SaqAI TTAA 7 cut(s) 492, 653, 945, 1425, 1532, 1865, 2301
SatI GCNGC 7 cut(s) 344, 693, 855, 1124, 1150, 1885, 2205
Sau3AI GATC 7 cut(s) 66, 1105, 1401, 1420, 1524, 2011, 2169
Sau96I GGNCC 7 cut(s) 337, 377, 1045, 1116, 1588, 1876, 2232
SchI GAGTC 2 cut(s) 722, 2418
ScrFI CCNGG 6 cut(s) 381, 740, 1023, 1967, 2115, 2369
SduI GDGCHC 2 cut(s) 1336, 2531
SexAI ACCWGGT 1 cut(s) 2367
SfaNI GCATC 9 cut(s) 48, 260, 466, 812, 1322, 1446, 1631, 1680, 1948
SfcI CTRYAG 1 cut(s) 1282
Sfr274I CTCGAG 3 cut(s) 1111, 1127, 1679
SfuI TTCGAA 1 cut(s) 1640
SinI GGWCC 6 cut(s) 337, 377, 1116, 1588, 1876, 2232
SlaI CTCGAG 3 cut(s) 1111, 1127, 1679
SmiMI CAYNNNNRTG 2 cut(s) 903, 1956
SphI GCATGC 1 cut(s) 350
SseBI AGGCCT 1 cut(s) 576
SsiI CCGC 6 cut(s) 854, 1444, 1596, 1620, 1766, 2275
SspI AATATT 3 cut(s) 131, 940, 1231
SspMI CTAG 7 cut(s) 353, 561, 660, 1067, 1172, 1577, 1796
SstI GAGCTC 1 cut(s) 2531
StuI AGGCCT 1 cut(s) 576
StyD4I CCNGG 6 cut(s) 379, 738, 1021, 1965, 2113, 2367
StyI CCWWGG 1 cut(s) 1048
TaaI ACNGT 5 cut(s) 452, 625, 671, 1328, 2353
TaiI ACGT 6 cut(s) 9, 171, 640, 921, 1205, 2385
TauI GCSGC 1 cut(s) 857
Tru1I TTAA 7 cut(s) 492, 653, 945, 1425, 1532, 1865, 2301
Tru9I TTAA 7 cut(s) 492, 653, 945, 1425, 1532, 1865, 2301
TscAI CASTG 2 cut(s) 1508, 1917
TseFI GTSAC 3 cut(s) 764, 1572, 2148
TseI GCWGC 6 cut(s) 343, 692, 1123, 1149, 1884, 2204
Tsp45I GTSAC 3 cut(s) 764, 1572, 2148
TspDTI ATGAA 8 cut(s) 138, 148, 692, 1013, 1157, 1383, 2301, 2316
TspGWI ACGGA 4 cut(s) 525, 542, 948, 1466
TspRI CASTG 2 cut(s) 1508, 1917
Tth111I GACNNNGTC 1 cut(s) 731
Van91I CCANNNNNTGG 2 cut(s) 1203, 1507
VpaK11BI GGWCC 6 cut(s) 337, 377, 1116, 1588, 1876, 2232
XapI RAATTY 6 cut(s) 304, 965, 1000, 1098, 2134, 2490
XbaI TCTAGA 1 cut(s) 1171
XceI RCATGY 5 cut(s) 350, 500, 714, 839, 904
XcmI CCANNNNNNNNNTGG 1 cut(s) 1460
XhoI CTCGAG 3 cut(s) 1111, 1127, 1679
XmiI GTMKAC 2 cut(s) 534, 1977
XmnI GAANNNNTTC 3 cut(s) 213, 624, 1920
XspI CTAG 7 cut(s) 353, 561, 660, 1067, 1172, 1577, 1796
Zsp2I ATGCAT 1 cut(s) 827
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.