AT4G09435

Toll - interleukin 1 - resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0016584
Physical Location & Seq
Forward (+)
5973083 .. 5974684
1602 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G09435.1

Sequence Viewer

Length: 1422 bp
ATGCGTGATCTCACATTTCTCAAGTTTTTAAAGCATGTGGTTGATATAACGTCCAAGCTGCAGCTCATTTCGGATGTTTCTAGTATCACTCATGGACTTAAGTTACTCCATTGGGACGCCTACCCTCTGGAAACCTTGCCTTTTAGTTTCCAATCAAGCACCCTAGTCGAAATCAATCTGCGATACAGCAACCTAAAACACTTTTGGGATGAAACCAAGGTCTATAGATCCAAGCTTCCGAACCTACGAAGACTAGATGTGACAGGCTCTACGAGTCTAGTAGAACTTCCAGATCTTTCAGATTCCATGAATCTTGAGGAGTTGATAATGGAAGGCTGCAGGAGCCTTAGGCAAACCCCATGGTCCTTAAACAGATTACCTTTGAGGAAACTCAATATGGTCAAGTGTGATAGCCTTATGGGTCTCTTGCTGGTTACTGACGACCATAATCAACCCAAGGCCAGCCGACCCAGCCCCTATCGACATATTAATTTGTTGCTTCTTGATACAGTAACAGCGCTTAGTTCTCTTACAGAATTATCCATCCAAGGCGAAATATCTGTTAAGTTATTGCACACTCTCATAGGAAGTGCAGAGCACTTGTCTTTTACTTGCGAGCAACAGATCCCTGATCAATTGAAAATTACGATGGCACAGAAAACAGGTTCAATTCAACCCTTGCACCTTATCAAAACACTCGTCATTGAGAGGTTCAACTACGGGGCGAGAGAAGCTCCTTTCAGTTGTCAGAGCTTTTCAAGCTTTCCTTGTTTGACTGAGCTAAAGCTCATCAACTTAAGCATCCGAGAAATCCCACAAGACATTGATTGTTTGTTGTCCTTAAGGAAAATGGACCTCACCGGTAATGATTTCGTTCATCTACCCAAAACCATGGCACAACTGACCAAGCTGGAATGTCTTACTCTTCGAAACTGCCGTCAGCTCAAAGCATTGCCACTACTTACTCCCACCCTCACTCTTCCTGGTCTAGACAACCAACCGAGGGGTTTGATAGAGCTTTGCATTGACAACTGCAAGAATCTTCAGTCACTGCAAGATCAGCTATTATGCTATAACACCAGTTTAGCATATTTAGACCTTAGCAACCATGATTTCGAGAGAATACCAACAAGCATTAGACACCTGTCCTCACTGAACACTCTCTGCCTCAAGAACTGCAAGAAACTCAAATATGTGGAAGAGCTTCCCCTGAGTCTCAACCATCTCTATGCACACGGCTGTGATTATCTGGAGAACGTCACCCTTTCCCCAAACCATACGATCAAACACCTCGATCTTAGAGATTGCCCCCGCCTGAAACAAAGCGAGCAGATAAAGACTCTGGTTTCAAACGAGAGACATGGTGAAGCGGTAAGTTTTCTCTTGTCGACCCGCTATTTCGTGATGCCTCTACTATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

473

Amino Acids

54.06

Weight (kDa)

7.88

Isoelectric Point (pI)

41.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 243 - 315 1.2e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1388
AciI CCGC 3 cut(s) 1312, 1370, 1393
AclWI GGATC 2 cut(s) 222, 619
AcuI CTGAAG 1 cut(s) 1028
AcyI GRCGYC 1 cut(s) 117
AfeI AGCGCT 1 cut(s) 519
AfiI CCNNNNNNNGG 1 cut(s) 1003
AflII CTTAAG 3 cut(s) 98, 796, 841
AgeI ACCGGT 1 cut(s) 860
AgsI TTSAA 6 cut(s) 640, 669, 674, 715, 759, 1350
AjnI CCWGG 1 cut(s) 982
AleI CACNNNNGTG 1 cut(s) 1239
Alw21I GWGCWC 1 cut(s) 600
Alw26I GTCTC 3 cut(s) 428, 1220, 1351
AlwI GGATC 2 cut(s) 222, 619
AlwNI CAGNNNCTG 1 cut(s) 1051
Aor51HI AGCGCT 1 cut(s) 519
AoxI GGCC 1 cut(s) 459
ApeKI GCWGC 3 cut(s) 58, 61, 336
ArsI GACNNNNNNTTYG 4 cut(s) 684, 716, 922, 954
AseI ATTAAT 1 cut(s) 489
AsiGI ACCGGT 1 cut(s) 860
Asp700I GAANNNNTTC 1 cut(s) 1203
AspLEI GCGC 1 cut(s) 520
AspS9I GGNCC 2 cut(s) 363, 853
AsuHPI GGTGA 3 cut(s) 850, 1252, 1376
AsuII TTCGAA 1 cut(s) 928
AvaII GGWCC 2 cut(s) 363, 853
AxyI CCTNAGG 1 cut(s) 347
BbsI GAAGAC 1 cut(s) 256
Bbv12I GWGCWC 1 cut(s) 600
BbvI GCAGC 3 cut(s) 45, 73, 323
BccI CCATC 3 cut(s) 551, 643, 1230
BceAI ACGGC 2 cut(s) 921, 1252
BcgI CGANNNNNNTGC 2 cut(s) 148, 182
BciT130I CCWGG 1 cut(s) 984
BclI TGATCA 1 cut(s) 631
BcoDI GTCTC 3 cut(s) 428, 1220, 1351
BfaI CTAG 5 cut(s) 81, 164, 254, 278, 989
BfmI CTRYAG 3 cut(s) 59, 223, 337
BfoI RGCGCY 1 cut(s) 521
BfrI CTTAAG 3 cut(s) 98, 796, 841
BglII AGATCT 1 cut(s) 292
BisI GCNGC 3 cut(s) 59, 62, 337
BlsI GCNGC 3 cut(s) 60, 63, 338
Bme1390I CCNGG 1 cut(s) 984
Bme18I GGWCC 2 cut(s) 363, 853
BmgT120I GGNCC 2 cut(s) 363, 853
BmiI GGNNCC 1 cut(s) 344
BmrFI CCNGG 1 cut(s) 984
BmsI GCATC 2 cut(s) 810, 1395
BoxI GACNNNNGTC 1 cut(s) 1144
BpiI GAAGAC 1 cut(s) 256
BplI GAGNNNNNCTC 2 cut(s) 718, 750
BpmI CTGGAG 1 cut(s) 1271
Bpu10I CCTNAGC 1 cut(s) 1100
Bpu14I TTCGAA 1 cut(s) 928
BpuEI CTTGAG 3 cut(s) 5, 335, 1154
BsaHI GRCGYC 1 cut(s) 117
BsaI GGTCTC 1 cut(s) 428
BsaJI CCNNGG 6 cut(s) 216, 359, 456, 547, 891, 1001
BsaWI WCCGGW 1 cut(s) 860
Bsc4I CCNNNNNNNGG 1 cut(s) 1003
Bse118I RCCGGY 1 cut(s) 860
Bse1I ACTGG 1 cut(s) 1080
Bse21I CCTNAGG 1 cut(s) 347
Bse3DI GCAATG 1 cut(s) 950
BseBI CCWGG 1 cut(s) 984
BseDI CCNNGG 6 cut(s) 216, 359, 456, 547, 891, 1001
BseGI GGATG 4 cut(s) 79, 214, 543, 801
BseLI CCNNNNNNNGG 1 cut(s) 1003
BseMI GCAATG 1 cut(s) 950
BseMII CTCAG 2 cut(s) 768, 1202
BseNI ACTGG 1 cut(s) 1080
BseRI GAGGAG 1 cut(s) 332
BseXI GCAGC 3 cut(s) 45, 73, 323
BseYI CCCAGC 1 cut(s) 470
BsgI GTGCAG 1 cut(s) 612
BshFI GGCC 1 cut(s) 461
BshTI ACCGGT 1 cut(s) 860
BsiHKAI GWGCWC 1 cut(s) 600
BsiSI CCGG 1 cut(s) 861
BslFI GGGAC 1 cut(s) 128
BslI CCNNNNNNNGG 1 cut(s) 1003
BsmAI GTCTC 3 cut(s) 428, 1220, 1351
BsmFI GGGAC 1 cut(s) 128
BsnI GGCC 1 cut(s) 461
Bso31I GGTCTC 1 cut(s) 428
Bsp119I TTCGAA 1 cut(s) 928
Bsp1286I GDGCHC 1 cut(s) 600
Bsp143I GATC 8 cut(s) 7, 227, 292, 624, 631, 1057, 1281, 1294
Bsp19I CCATGG 2 cut(s) 359, 891
BspACI CCGC 3 cut(s) 1312, 1370, 1393
BspANI GGCC 1 cut(s) 461
BspCNI CTCAG 2 cut(s) 769, 1203
BspLI GGNNCC 1 cut(s) 344
BspMAI CTGCAG 2 cut(s) 63, 341
BspPI GGATC 2 cut(s) 222, 619
BspQI GCTCTTC 1 cut(s) 1194
BspT104I TTCGAA 1 cut(s) 928
BspTI CTTAAG 3 cut(s) 98, 796, 841
BspTNI GGTCTC 1 cut(s) 428
BsrDI GCAATG 1 cut(s) 950
BsrFI RCCGGY 1 cut(s) 860
BsrI ACTGG 1 cut(s) 1080
BssAI RCCGGY 1 cut(s) 860
BssECI CCNNGG 6 cut(s) 216, 359, 456, 547, 891, 1001
BssMI GATC 8 cut(s) 7, 227, 292, 624, 631, 1057, 1281, 1294
BssNI GRCGYC 1 cut(s) 117
BssT1I CCWWGG 5 cut(s) 216, 359, 456, 547, 891
Bst2UI CCWGG 1 cut(s) 984
Bst4CI ACNGT 1 cut(s) 511
Bst6I CTCTTC 3 cut(s) 930, 984, 1194
BstACI GRCGYC 1 cut(s) 117
BstAFI CTTAAG 3 cut(s) 98, 796, 841
BstBI TTCGAA 1 cut(s) 928
BstC8I GCNNGC 3 cut(s) 463, 617, 1328
BstDEI CTNAG 6 cut(s) 347, 521, 777, 1100, 1211, 1298
BstDSI CCRYGG 2 cut(s) 359, 891
BstF5I GGATG 4 cut(s) 79, 214, 543, 801
BstH2I RGCGCY 1 cut(s) 521
BstHHI GCGC 1 cut(s) 520
BstKTI GATC 8 cut(s) 10, 230, 295, 627, 634, 1060, 1284, 1297
BstMAI GTCTC 3 cut(s) 428, 1220, 1351
BstMBI GATC 8 cut(s) 7, 227, 292, 624, 631, 1057, 1281, 1294
BstMWI GCNNNNNNNGC 5 cut(s) 342, 471, 731, 759, 1060
BstNI CCWGG 1 cut(s) 984
BstNSI RCATGY 1 cut(s) 38
BstPAI GACNNNNGTC 1 cut(s) 1144
BstSCI CCNGG 1 cut(s) 982
BstSFI CTRYAG 3 cut(s) 59, 223, 337
BstV1I GCAGC 3 cut(s) 45, 73, 323
BstV2I GAAGAC 1 cut(s) 256
BstX2I RGATCY 3 cut(s) 227, 292, 624
BstXI CCANNNNNNTGG 1 cut(s) 892
BstYI RGATCY 3 cut(s) 227, 292, 624
Bsu36I CCTNAGG 1 cut(s) 347
BsuRI GGCC 1 cut(s) 461
BtgI CCRYGG 2 cut(s) 359, 891
BtsCI GGATG 4 cut(s) 79, 214, 543, 801
BtsI GCAGTG 1 cut(s) 1049
BtsIMutI CAGTG 2 cut(s) 1049, 1151
Cac8I GCNNGC 3 cut(s) 463, 617, 1328
CaiI CAGNNNCTG 1 cut(s) 1051
CfoI GCGC 1 cut(s) 520
Cfr10I RCCGGY 1 cut(s) 860
Cfr13I GGNCC 2 cut(s) 363, 853
CseI GACGC 1 cut(s) 125
CspAI ACCGGT 1 cut(s) 860
CviAII CATG 7 cut(s) 35, 92, 307, 360, 892, 1109, 1361
DdeI CTNAG 6 cut(s) 347, 521, 777, 1100, 1211, 1298
DpnI GATC 8 cut(s) 9, 229, 294, 626, 633, 1059, 1283, 1296
DpnII GATC 8 cut(s) 7, 227, 292, 624, 631, 1057, 1281, 1294
DraI TTTAAA 1 cut(s) 30
Eam1104I CTCTTC 3 cut(s) 930, 984, 1194
EarI CTCTTC 3 cut(s) 930, 984, 1194
Eco130I CCWWGG 5 cut(s) 216, 359, 456, 547, 891
Eco31I GGTCTC 1 cut(s) 428
Eco47I GGWCC 2 cut(s) 363, 853
Eco47III AGCGCT 1 cut(s) 519
Eco57I CTGAAG 1 cut(s) 1028
Eco81I CCTNAGG 1 cut(s) 347
EcoRII CCWGG 1 cut(s) 982
EcoT14I CCWWGG 5 cut(s) 216, 359, 456, 547, 891
ErhI CCWWGG 5 cut(s) 216, 359, 456, 547, 891
FaeI CATG 7 cut(s) 38, 95, 310, 363, 895, 1112, 1364
FalI AAGNNNNNCTT 2 cut(s) 751, 783
FaqI GGGAC 1 cut(s) 128
FatI CATG 7 cut(s) 34, 91, 306, 359, 891, 1108, 1360
FauI CCCGC 2 cut(s) 1319, 1400
FbaI TGATCA 1 cut(s) 631
FblI GTMKAC 1 cut(s) 1388
Fnu4HI GCNGC 3 cut(s) 59, 62, 337
FokI GGATG 4 cut(s) 86, 221, 530, 788
Fsp4HI GCNGC 3 cut(s) 59, 62, 337
FspBI CTAG 5 cut(s) 81, 164, 254, 278, 989
GlaI GCGC 1 cut(s) 519
GluI GCNGC 3 cut(s) 59, 62, 337
GsaI CCCAGC 1 cut(s) 474
GsuI CTGGAG 1 cut(s) 1271
HaeII RGCGCY 1 cut(s) 521
HaeIII GGCC 1 cut(s) 461
HapII CCGG 1 cut(s) 861
HgaI GACGC 1 cut(s) 125
HhaI GCGC 1 cut(s) 520
Hin1I GRCGYC 1 cut(s) 117
Hin1II CATG 7 cut(s) 38, 95, 310, 363, 895, 1112, 1364
Hin6I GCGC 1 cut(s) 518
HinP1I GCGC 1 cut(s) 518
HincII GTYRAC 1 cut(s) 1389
HindII GTYRAC 1 cut(s) 1389
HindIII AAGCTT 2 cut(s) 233, 760
HinfI GANTC 6 cut(s) 274, 302, 310, 1039, 1213, 1339
HpaII CCGG 1 cut(s) 861
HphI GGTGA 3 cut(s) 850, 1252, 1376
Hpy166II GTNNAC 1 cut(s) 1389
Hpy188I TCNGA 5 cut(s) 73, 240, 301, 750, 806
Hpy188III TCNNGA 9 cut(s) 128, 290, 314, 503, 989, 1117, 1171, 1250, 1402
Hpy8I GTNNAC 1 cut(s) 1389
HpyAV CCTTC 1 cut(s) 326
HpyCH4III ACNGT 1 cut(s) 511
HpyCH4IV ACGT 2 cut(s) 50, 1257
HpyF10VI GCNNNNNNNGC 5 cut(s) 342, 471, 731, 759, 1060
HpyF3I CTNAG 6 cut(s) 347, 521, 777, 1100, 1211, 1298
HpySE526I ACGT 2 cut(s) 50, 1257
Hsp92I GRCGYC 1 cut(s) 117
Hsp92II CATG 7 cut(s) 38, 95, 310, 363, 895, 1112, 1364
HspAI GCGC 1 cut(s) 518
Ksp22I TGATCA 1 cut(s) 631
Kzo9I GATC 8 cut(s) 7, 227, 292, 624, 631, 1057, 1281, 1294
LguI GCTCTTC 1 cut(s) 1194
LmnI GCTCC 2 cut(s) 342, 739
Lsp1109I GCAGC 3 cut(s) 45, 73, 323
LweI GCATC 2 cut(s) 810, 1395
MaeI CTAG 5 cut(s) 81, 164, 254, 278, 989
MaeII ACGT 2 cut(s) 50, 1257
MaeIII GTNAC 6 cut(s) 102, 259, 433, 511, 1047, 1258
MalI GATC 8 cut(s) 9, 229, 294, 626, 633, 1059, 1283, 1296
MboI GATC 8 cut(s) 7, 227, 292, 624, 631, 1057, 1281, 1294
MboII GAAGA 5 cut(s) 261, 917, 971, 1034, 1211
MfeI CAATTG 1 cut(s) 635
MflI RGATCY 3 cut(s) 227, 292, 624
MhlI GDGCHC 1 cut(s) 600
MluCI AATT 5 cut(s) 490, 536, 635, 642, 669
MlyI GAGTC 3 cut(s) 283, 1222, 1333
MroXI GAANNNNTTC 1 cut(s) 1203
MseI TTAA 7 cut(s) 29, 99, 368, 489, 564, 797, 842
MslI CAYNNNNRTG 2 cut(s) 1227, 1239
MspCI CTTAAG 3 cut(s) 98, 796, 841
MspI CCGG 1 cut(s) 861
MspR9I CCNGG 1 cut(s) 984
MunI CAATTG 1 cut(s) 635
MvaI CCWGG 1 cut(s) 984
MwoI GCNNNNNNNGC 5 cut(s) 342, 471, 731, 759, 1060
NcoI CCATGG 2 cut(s) 359, 891
NdeII GATC 8 cut(s) 7, 227, 292, 624, 631, 1057, 1281, 1294
NlaIII CATG 7 cut(s) 38, 95, 310, 363, 895, 1112, 1364
NlaIV GGNNCC 1 cut(s) 344
NmuCI GTSAC 3 cut(s) 259, 1047, 1258
NspI RCATGY 1 cut(s) 38
NspV TTCGAA 1 cut(s) 928
OliI CACNNNNGTG 1 cut(s) 1239
PciSI GCTCTTC 1 cut(s) 1194
PcsI WCGNNNNNNNCGW 1 cut(s) 934
PdmI GAANNNNTTC 1 cut(s) 1203
PfeI GAWTC 3 cut(s) 302, 310, 1039
PinAI ACCGGT 1 cut(s) 860
PkrI GCNGC 3 cut(s) 60, 63, 338
PleI GAGTC 3 cut(s) 282, 1221, 1333
PpsI GAGTC 3 cut(s) 282, 1221, 1333
PshAI GACNNNNGTC 1 cut(s) 1144
PshBI ATTAAT 1 cut(s) 489
Psp6I CCWGG 1 cut(s) 982
PspFI CCCAGC 1 cut(s) 470
PspGI CCWGG 1 cut(s) 982
PspN4I GGNNCC 1 cut(s) 344
PspPI GGNCC 2 cut(s) 363, 853
PstI CTGCAG 2 cut(s) 63, 341
PstNI CAGNNNCTG 1 cut(s) 1051
PsuI RGATCY 3 cut(s) 227, 292, 624
RseI CAYNNNNRTG 2 cut(s) 1227, 1239
SalI GTCGAC 1 cut(s) 1387
SapI GCTCTTC 1 cut(s) 1194
SaqAI TTAA 7 cut(s) 29, 99, 368, 489, 564, 797, 842
SatI GCNGC 3 cut(s) 59, 62, 337
Sau3AI GATC 8 cut(s) 7, 227, 292, 624, 631, 1057, 1281, 1294
Sau96I GGNCC 2 cut(s) 363, 853
SchI GAGTC 3 cut(s) 283, 1222, 1333
ScrFI CCNGG 1 cut(s) 984
SduI GDGCHC 1 cut(s) 600
SfaNI GCATC 2 cut(s) 810, 1395
SfcI CTRYAG 3 cut(s) 59, 223, 337
SfuI TTCGAA 1 cut(s) 928
SinI GGWCC 2 cut(s) 363, 853
SmiMI CAYNNNNRTG 2 cut(s) 1227, 1239
SmlI CTYRAG 6 cut(s) 20, 98, 314, 796, 841, 1169
SmoI CTYRAG 6 cut(s) 20, 98, 314, 796, 841, 1169
Sse9I AATT 5 cut(s) 490, 536, 635, 642, 669
SsiI CCGC 3 cut(s) 1312, 1370, 1393
SspMI CTAG 5 cut(s) 81, 164, 254, 278, 989
StyD4I CCNGG 1 cut(s) 982
StyI CCWWGG 5 cut(s) 216, 359, 456, 547, 891
TaaI ACNGT 1 cut(s) 511
TaiI ACGT 2 cut(s) 53, 1260
TaqI TCGA 6 cut(s) 168, 481, 928, 1116, 1293, 1388
TasI AATT 5 cut(s) 490, 536, 635, 642, 669
TfiI GAWTC 3 cut(s) 302, 310, 1039
Tru1I TTAA 7 cut(s) 29, 99, 368, 489, 564, 797, 842
Tru9I TTAA 7 cut(s) 29, 99, 368, 489, 564, 797, 842
TscAI CASTG 2 cut(s) 1056, 1158
TseFI GTSAC 3 cut(s) 259, 1047, 1258
TseI GCWGC 3 cut(s) 58, 61, 336
Tsp45I GTSAC 3 cut(s) 259, 1047, 1258
TspDTI ATGAA 3 cut(s) 225, 323, 866
TspRI CASTG 2 cut(s) 1056, 1158
Vha464I CTTAAG 3 cut(s) 98, 796, 841
VpaK11BI GGWCC 2 cut(s) 363, 853
VspI ATTAAT 1 cut(s) 489
XbaI TCTAGA 1 cut(s) 988
XceI RCATGY 1 cut(s) 38
XmiI GTMKAC 1 cut(s) 1388
XmnI GAANNNNTTC 1 cut(s) 1203
XspI CTAG 5 cut(s) 81, 164, 254, 278, 989
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.