AT4G10250

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0020267
Physical Location & Seq
Forward (+)
6370338 .. 6371372
1035 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G10250.1

Sequence Viewer

Length: 588 bp
ATGATGAAGCACTTGCTAAGCATCTTCTTCATAGGAGCTTTGTTGCTTGGAAACATCAAAACAAGTGAAGGGTCTTTGTCTTCTGCACTTGAAACTACTCCAGGCAGCTTGCTATCCGATCTCTGGCTAGACCGGTTCCCTGATCCATTCAAGATCTTGGAGAGAATCCCATTAGGACTCGAGAGGGACACAAGCGTGGCTCTGTCTCCAGCAAGAGTAGACTGGAAAGAAACAGCAGAAGGGCATGAGATAATGCTCGATATCCCCGGTTTGAAGAAGGATGAAGTAAAGATAGAAGTGGAGGAGAACGGAGTTTTACGAGTCAGTGGAGAGAGGAAAAGAGAAGAAGAGAAGAAAGGAGATCAATGGCATAGAGTGGAGAGATCATATGGAAAATTCTGGAGACAGTTCAAGCTACCTGATAATGTAGATATGGAATCAGTCAAGGCCAAGCTTGAGAATGGTGTGCTCACTATTAACCTCACGAAACTATCTCCTGAAAAAGTTAAGGGTCCAAGAGTTGTTAACATTGCAGCTGAAGAAGACCAAACTGCAAAAATCAGCTCTTCTGAATCCAAAGAACTCTGA

Protein Analysis

195

Amino Acids

22.0

Weight (kDa)

5.58

Isoelectric Point (pI)

36.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 74 - 177 5.2e-31 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020267)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10250
malus_domestica MD05G1183400.v1.1 MD10G1171200.v1.1
prunus_persica Prupe.8G000400_v2.0.a1
pyrus_communis pycom05g16840 pycom10g14760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 219
AclWI GGATC 1 cut(s) 137
AcsI RAATTY 1 cut(s) 395
AcuI CTGAAG 1 cut(s) 558
AfiI CCNNNNNNNGG 1 cut(s) 123
AgeI ACCGGT 1 cut(s) 132
AgsI TTSAA 4 cut(s) 92, 151, 274, 412
AjnI CCWGG 1 cut(s) 100
AleI CACNNNNGTG 1 cut(s) 194
AluBI AGCT 6 cut(s) 38, 108, 415, 454, 536, 564
AluI AGCT 6 cut(s) 38, 108, 415, 454, 536, 564
Alw21I GWGCWC 1 cut(s) 471
Alw26I GTCTC 2 cut(s) 210, 397
AlwI GGATC 1 cut(s) 137
Ama87I CYCGRG 1 cut(s) 179
AoxI GGCC 1 cut(s) 447
ApeKI GCWGC 2 cut(s) 105, 533
ApoI RAATTY 1 cut(s) 395
AsiGI ACCGGT 1 cut(s) 132
AspS9I GGNCC 1 cut(s) 512
AsuC2I CCSGG 1 cut(s) 267
AvaI CYCGRG 1 cut(s) 179
AvaII GGWCC 1 cut(s) 512
BbsI GAAGAC 2 cut(s) 72, 549
Bbv12I GWGCWC 1 cut(s) 471
BbvI GCAGC 2 cut(s) 117, 545
BciT130I CCWGG 1 cut(s) 102
BcnI CCSGG 1 cut(s) 267
BcoDI GTCTC 2 cut(s) 210, 397
BfaI CTAG 1 cut(s) 128
BglII AGATCT 1 cut(s) 153
BisI GCNGC 2 cut(s) 106, 534
BlpI GCTNAGC 1 cut(s) 17
BlsI GCNGC 2 cut(s) 107, 535
Bme1390I CCNGG 2 cut(s) 102, 267
Bme18I GGWCC 1 cut(s) 512
BmeT110I CYCGRG 1 cut(s) 179
BmgT120I GGNCC 1 cut(s) 512
BmiI GGNNCC 2 cut(s) 137, 513
BmrFI CCNGG 2 cut(s) 102, 267
BmsI GCATC 1 cut(s) 30
BpiI GAAGAC 2 cut(s) 72, 549
BpmI CTGGAG 3 cut(s) 84, 192, 421
Bpu1102I GCTNAGC 1 cut(s) 17
BpuEI CTTGAG 1 cut(s) 476
BpuMI CCSGG 1 cut(s) 267
BsaJI CCNNGG 1 cut(s) 265
BsaWI WCCGGW 1 cut(s) 132
Bsc4I CCNNNNNNNGG 1 cut(s) 123
Bse118I RCCGGY 1 cut(s) 132
Bse1I ACTGG 1 cut(s) 227
Bse3DI GCAATG 1 cut(s) 528
BseBI CCWGG 1 cut(s) 102
BseDI CCNNGG 1 cut(s) 265
BseGI GGATG 1 cut(s) 286
BseLI CCNNNNNNNGG 1 cut(s) 123
BseMI GCAATG 1 cut(s) 528
BseNI ACTGG 1 cut(s) 227
BseRI GAGGAG 1 cut(s) 317
BseXI GCAGC 2 cut(s) 117, 545
BsgI GTGCAG 1 cut(s) 69
BshFI GGCC 1 cut(s) 449
BshTI ACCGGT 1 cut(s) 132
BsiHKAI GWGCWC 1 cut(s) 471
BsiHKCI CYCGRG 1 cut(s) 179
BsiSI CCGG 2 cut(s) 133, 267
BslFI GGGAC 1 cut(s) 200
BslI CCNNNNNNNGG 1 cut(s) 123
BsmAI GTCTC 2 cut(s) 210, 397
BsmFI GGGAC 1 cut(s) 200
BsnI GGCC 1 cut(s) 449
BsoBI CYCGRG 1 cut(s) 179
Bsp1286I GDGCHC 1 cut(s) 471
Bsp143I GATC 5 cut(s) 118, 142, 153, 361, 383
Bsp1720I GCTNAGC 1 cut(s) 17
BspANI GGCC 1 cut(s) 449
BspLI GGNNCC 2 cut(s) 137, 513
BspPI GGATC 1 cut(s) 137
BspQI GCTCTTC 1 cut(s) 571
BsrDI GCAATG 1 cut(s) 528
BsrFI RCCGGY 1 cut(s) 132
BsrI ACTGG 1 cut(s) 227
BssAI RCCGGY 1 cut(s) 132
BssECI CCNNGG 1 cut(s) 265
BssMI GATC 5 cut(s) 118, 142, 153, 361, 383
Bst2UI CCWGG 1 cut(s) 102
Bst4CI ACNGT 1 cut(s) 408
Bst6I CTCTTC 2 cut(s) 342, 571
BstC8I GCNNGC 1 cut(s) 110
BstDEI CTNAG 1 cut(s) 17
BstF5I GGATG 1 cut(s) 286
BstKTI GATC 5 cut(s) 121, 145, 156, 364, 386
BstMAI GTCTC 2 cut(s) 210, 397
BstMBI GATC 5 cut(s) 118, 142, 153, 361, 383
BstNI CCWGG 1 cut(s) 102
BstSCI CCNGG 2 cut(s) 100, 265
BstV1I GCAGC 2 cut(s) 117, 545
BstV2I GAAGAC 2 cut(s) 72, 549
BstX2I RGATCY 1 cut(s) 153
BstYI RGATCY 1 cut(s) 153
BsuRI GGCC 1 cut(s) 449
BtsCI GGATG 1 cut(s) 286
BtsIMutI CAGTG 1 cut(s) 331
Cac8I GCNNGC 1 cut(s) 110
Cfr10I RCCGGY 1 cut(s) 132
Cfr13I GGNCC 1 cut(s) 512
CspAI ACCGGT 1 cut(s) 132
CviAII CATG 1 cut(s) 245
CviJI RGCY 9 cut(s) 38, 108, 127, 200, 415, 449, 454, 536, 564
CviKI_1 RGCY 9 cut(s) 38, 108, 127, 200, 415, 449, 454, 536, 564
DdeI CTNAG 1 cut(s) 17
DpnI GATC 5 cut(s) 120, 144, 155, 363, 385
DpnII GATC 5 cut(s) 118, 142, 153, 361, 383
Eam1104I CTCTTC 2 cut(s) 342, 571
EarI CTCTTC 2 cut(s) 342, 571
Eco32I GATATC 1 cut(s) 262
Eco47I GGWCC 1 cut(s) 512
Eco57I CTGAAG 1 cut(s) 558
Eco88I CYCGRG 1 cut(s) 179
EcoRII CCWGG 1 cut(s) 100
EcoRV GATATC 1 cut(s) 262
FaeI CATG 1 cut(s) 248
FaiI YATR 6 cut(s) 32, 246, 372, 388, 390, 434
FaqI GGGAC 1 cut(s) 200
FatI CATG 1 cut(s) 244
FauNDI CATATG 1 cut(s) 388
FblI GTMKAC 1 cut(s) 219
Fnu4HI GCNGC 2 cut(s) 106, 534
FokI GGATG 1 cut(s) 293
Fsp4HI GCNGC 2 cut(s) 106, 534
FspBI CTAG 1 cut(s) 128
GluI GCNGC 2 cut(s) 106, 534
GsuI CTGGAG 3 cut(s) 84, 192, 421
HaeIII GGCC 1 cut(s) 449
HapII CCGG 2 cut(s) 133, 267
Hin1II CATG 1 cut(s) 248
HincII GTYRAC 1 cut(s) 526
HindII GTYRAC 1 cut(s) 526
HindIII AAGCTT 1 cut(s) 452
HinfI GANTC 5 cut(s) 165, 177, 321, 437, 572
HpaI GTTAAC 1 cut(s) 526
HpaII CCGG 2 cut(s) 133, 267
Hpy166II GTNNAC 2 cut(s) 220, 526
Hpy188I TCNGA 3 cut(s) 118, 571, 587
Hpy188III TCNNGA 5 cut(s) 151, 181, 400, 484, 497
Hpy8I GTNNAC 2 cut(s) 220, 526
HpyAV CCTTC 3 cut(s) 62, 233, 271
HpyCH4III ACNGT 1 cut(s) 408
HpyCH4V TGCA 3 cut(s) 86, 533, 554
HpyF3I CTNAG 1 cut(s) 17
Hsp92II CATG 1 cut(s) 248
KspAI GTTAAC 1 cut(s) 526
Kzo9I GATC 5 cut(s) 118, 142, 153, 361, 383
LguI GCTCTTC 1 cut(s) 571
LmnI GCTCC 1 cut(s) 35
Lsp1109I GCAGC 2 cut(s) 117, 545
LweI GCATC 1 cut(s) 30
MaeI CTAG 1 cut(s) 128
MalI GATC 5 cut(s) 120, 144, 155, 363, 385
MboI GATC 5 cut(s) 118, 142, 153, 361, 383
MflI RGATCY 1 cut(s) 153
MhlI GDGCHC 1 cut(s) 471
MluCI AATT 1 cut(s) 395
MlyI GAGTC 2 cut(s) 171, 330
MnlI CCTC 4 cut(s) 177, 295, 327, 491
MseI TTAA 3 cut(s) 477, 507, 525
MslI CAYNNNNRTG 1 cut(s) 194
MspA1I CMGCKG 1 cut(s) 536
MspI CCGG 2 cut(s) 133, 267
MspR9I CCNGG 2 cut(s) 102, 267
MvaI CCWGG 1 cut(s) 102
NciI CCSGG 1 cut(s) 267
NdeI CATATG 1 cut(s) 388
NdeII GATC 5 cut(s) 118, 142, 153, 361, 383
NlaIII CATG 1 cut(s) 248
NlaIV GGNNCC 2 cut(s) 137, 513
OliI CACNNNNGTG 1 cut(s) 194
PaeR7I CTCGAG 1 cut(s) 179
PciSI GCTCTTC 1 cut(s) 571
PfeI GAWTC 3 cut(s) 165, 437, 572
PinAI ACCGGT 1 cut(s) 132
PkrI GCNGC 2 cut(s) 107, 535
PleI GAGTC 2 cut(s) 171, 329
PpsI GAGTC 2 cut(s) 171, 329
Psp6I CCWGG 1 cut(s) 100
PspGI CCWGG 1 cut(s) 100
PspN4I GGNNCC 2 cut(s) 137, 513
PspPI GGNCC 1 cut(s) 512
PsuI RGATCY 1 cut(s) 153
PvuII CAGCTG 1 cut(s) 536
RseI CAYNNNNRTG 1 cut(s) 194
SapI GCTCTTC 1 cut(s) 571
SaqAI TTAA 3 cut(s) 477, 507, 525
SatI GCNGC 2 cut(s) 106, 534
Sau3AI GATC 5 cut(s) 118, 142, 153, 361, 383
Sau96I GGNCC 1 cut(s) 512
SchI GAGTC 2 cut(s) 171, 330
ScrFI CCNGG 2 cut(s) 102, 267
SduI GDGCHC 1 cut(s) 471
SetI ASST 8 cut(s) 40, 110, 417, 421, 456, 483, 538, 566
SfaNI GCATC 1 cut(s) 30
Sfr274I CTCGAG 1 cut(s) 179
SinI GGWCC 1 cut(s) 512
SlaI CTCGAG 1 cut(s) 179
SmiMI CAYNNNNRTG 1 cut(s) 194
SmlI CTYRAG 2 cut(s) 179, 455
SmoI CTYRAG 2 cut(s) 179, 455
Sse9I AATT 1 cut(s) 395
SspMI CTAG 1 cut(s) 128
StyD4I CCNGG 2 cut(s) 100, 265
TaaI ACNGT 1 cut(s) 408
TaqI TCGA 2 cut(s) 180, 258
TasI AATT 1 cut(s) 395
TfiI GAWTC 3 cut(s) 165, 437, 572
Tru1I TTAA 3 cut(s) 477, 507, 525
Tru9I TTAA 3 cut(s) 477, 507, 525
TscAI CASTG 1 cut(s) 331
TseI GCWGC 2 cut(s) 105, 533
TspDTI ATGAA 3 cut(s) 19, 20, 297
TspGWI ACGGA 1 cut(s) 324
TspRI CASTG 1 cut(s) 331
VpaK11BI GGWCC 1 cut(s) 512
XapI RAATTY 1 cut(s) 395
XhoI CTCGAG 1 cut(s) 179
XmiI GTMKAC 1 cut(s) 219
XspI CTAG 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.