pycom05g16840

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0020267
Physical Location & Seq
Reverse (-)
20124681 .. 20125368
688 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g16840.1

Sequence Viewer

Length: 483 bp
ATGAGGAAACAACCTTCTCTCAATATTGTCATCACCTTGTGCTTTCTCCTTTTTCTTTCTAGGTCATCCAATGGATCCTTGCTGCCGTTCATGGACCGACCAGAAACTAGTTCTCTGGTCGATCTCTTGTCGGAGCGCTTTCCAGATCCGTTCCGAGTGCTAGAACAGCTCCCATTTGAACTTGAAAAAGAAGACAGCCTGCTGGCACTCTCACCAGCAAAAGTGGACTGGAAAGAAACGCCGGAGGGTCATGTTATAATGTTAGATGTGCCGGGACTCAACAAGGAGGAGCTGAAAATCGAGGTGGAGGAGAACCGGGTTTTGAGAGTGACATCTCTACCACCGGAGGAGGAGGAGATCAGGGTGAACCTGCCAAGCTCAAGGGTAGCGAGCCCAAGCAAGAGCTGTAACTTAGAAAGCAAGGACATTAAGTGCCTTGCTTCAAGTTTGGCTGATAAGACTCAATTTCATTTTGTGTCTTGA

Protein Analysis

161

Amino Acids

18.09

Weight (kDa)

4.96

Isoelectric Point (pI)

68.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 76 - 118 3e-07 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020267)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10250
malus_domestica MD05G1183400.v1.1 MD10G1171200.v1.1
prunus_persica Prupe.8G000400_v2.0.a1
pyrus_communis pycom05g16840 pycom10g14760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 257
Acc36I ACCTGC 1 cut(s) 378
AclWI GGATC 3 cut(s) 69, 82, 140
AdeI CACNNNGTG 1 cut(s) 39
AfeI AGCGCT 1 cut(s) 137
AgsI TTSAA 3 cut(s) 179, 185, 444
AhlI ACTAGT 1 cut(s) 107
AluBI AGCT 4 cut(s) 169, 292, 378, 405
AluI AGCT 4 cut(s) 169, 292, 378, 405
AlwI GGATC 3 cut(s) 69, 82, 140
Aor51HI AGCGCT 1 cut(s) 137
ApeKI GCWGC 1 cut(s) 82
AspLEI GCGC 1 cut(s) 138
AspS9I GGNCC 1 cut(s) 94
AsuC2I CCSGG 2 cut(s) 273, 317
AsuHPI GGTGA 3 cut(s) 25, 204, 376
AvaII GGWCC 1 cut(s) 94
BamHI GGATCC 1 cut(s) 74
BanII GRGCYC 1 cut(s) 395
BbsI GAAGAC 1 cut(s) 198
BbvI GCAGC 1 cut(s) 69
BceAI ACGGC 1 cut(s) 70
BcnI CCSGG 2 cut(s) 273, 317
BcuI ACTAGT 1 cut(s) 107
BfaI CTAG 3 cut(s) 60, 108, 161
BfoI RGCGCY 1 cut(s) 139
BfuAI ACCTGC 1 cut(s) 378
BisI GCNGC 1 cut(s) 83
BlsI GCNGC 1 cut(s) 84
Bme1390I CCNGG 2 cut(s) 273, 317
Bme18I GGWCC 1 cut(s) 94
BmgT120I GGNCC 1 cut(s) 94
BmiI GGNNCC 1 cut(s) 76
BmrFI CCNGG 2 cut(s) 273, 317
BpiI GAAGAC 1 cut(s) 198
BpuEI CTTGAG 1 cut(s) 364
BpuMI CCSGG 2 cut(s) 273, 317
BsaWI WCCGGW 1 cut(s) 343
Bse1I ACTGG 1 cut(s) 233
BseGI GGATG 1 cut(s) 65
BseNI ACTGG 1 cut(s) 233
BseRI GAGGAG 5 cut(s) 302, 323, 362, 365, 368
BseXI GCAGC 1 cut(s) 69
BsiSI CCGG 4 cut(s) 242, 272, 316, 344
BslFI GGGAC 1 cut(s) 288
BsmFI GGGAC 1 cut(s) 288
Bsp1286I GDGCHC 1 cut(s) 395
Bsp143I GATC 4 cut(s) 74, 121, 145, 357
BspLI GGNNCC 1 cut(s) 76
BspMI ACCTGC 1 cut(s) 378
BspPI GGATC 3 cut(s) 69, 82, 140
BsrI ACTGG 1 cut(s) 233
BssMI GATC 4 cut(s) 74, 121, 145, 357
BstC8I GCNNGC 3 cut(s) 200, 204, 391
BstDEI CTNAG 1 cut(s) 412
BstF5I GGATG 1 cut(s) 65
BstH2I RGCGCY 1 cut(s) 139
BstHHI GCGC 1 cut(s) 138
BstKTI GATC 4 cut(s) 77, 124, 148, 360
BstMBI GATC 4 cut(s) 74, 121, 145, 357
BstMWI GCNNNNNNNGC 1 cut(s) 166
BstSCI CCNGG 2 cut(s) 271, 315
BstV1I GCAGC 1 cut(s) 69
BstV2I GAAGAC 1 cut(s) 198
BstX2I RGATCY 2 cut(s) 74, 145
BstYI RGATCY 2 cut(s) 74, 145
BtsCI GGATG 1 cut(s) 65
BveI ACCTGC 1 cut(s) 378
Cac8I GCNNGC 3 cut(s) 200, 204, 391
CfoI GCGC 1 cut(s) 138
Cfr13I GGNCC 1 cut(s) 94
CviAII CATG 2 cut(s) 91, 251
CviJI RGCY 7 cut(s) 169, 198, 292, 378, 393, 405, 452
CviKI_1 RGCY 7 cut(s) 169, 198, 292, 378, 393, 405, 452
DdeI CTNAG 1 cut(s) 412
DpnI GATC 4 cut(s) 76, 123, 147, 359
DpnII GATC 4 cut(s) 74, 121, 145, 357
DraIII CACNNNGTG 1 cut(s) 39
Eco24I GRGCYC 1 cut(s) 395
Eco47I GGWCC 1 cut(s) 94
Eco47III AGCGCT 1 cut(s) 137
EcoT38I GRGCYC 1 cut(s) 395
FaeI CATG 2 cut(s) 94, 254
FaiI YATR 3 cut(s) 92, 252, 257
FaqI GGGAC 1 cut(s) 288
FatI CATG 2 cut(s) 90, 250
Fnu4HI GCNGC 1 cut(s) 83
FokI GGATG 1 cut(s) 52
FriOI GRGCYC 1 cut(s) 395
Fsp4HI GCNGC 1 cut(s) 83
FspBI CTAG 3 cut(s) 60, 108, 161
GlaI GCGC 1 cut(s) 137
GluI GCNGC 1 cut(s) 83
HaeII RGCGCY 1 cut(s) 139
HapII CCGG 4 cut(s) 242, 272, 316, 344
HhaI GCGC 1 cut(s) 138
Hin1II CATG 2 cut(s) 94, 254
Hin6I GCGC 1 cut(s) 136
HinP1I GCGC 1 cut(s) 136
HinfI GANTC 2 cut(s) 276, 460
HpaII CCGG 4 cut(s) 242, 272, 316, 344
HphI GGTGA 3 cut(s) 25, 204, 376
Hpy166II GTNNAC 2 cut(s) 226, 367
Hpy188I TCNGA 2 cut(s) 133, 155
Hpy188III TCNNGA 2 cut(s) 143, 480
Hpy8I GTNNAC 2 cut(s) 226, 367
HpyAV CCTTC 1 cut(s) 24
HpyF10VI GCNNNNNNNGC 1 cut(s) 166
HpyF3I CTNAG 1 cut(s) 412
Hsp92II CATG 2 cut(s) 94, 254
HspAI GCGC 1 cut(s) 136
Kzo9I GATC 4 cut(s) 74, 121, 145, 357
LmnI GCTCC 3 cut(s) 133, 174, 289
Lsp1109I GCAGC 1 cut(s) 69
MaeI CTAG 3 cut(s) 60, 108, 161
MaeIII GTNAC 2 cut(s) 328, 407
MalI GATC 4 cut(s) 76, 123, 147, 359
MboI GATC 4 cut(s) 74, 121, 145, 357
MboII GAAGA 1 cut(s) 203
MflI RGATCY 2 cut(s) 74, 145
MhlI GDGCHC 1 cut(s) 395
MluCI AATT 1 cut(s) 464
MlyI GAGTC 2 cut(s) 270, 454
MmeI TCCRAC 1 cut(s) 111
MnlI CCTC 7 cut(s) 238, 280, 295, 301, 340, 343, 346
MseI TTAA 1 cut(s) 429
MspI CCGG 4 cut(s) 242, 272, 316, 344
MspR9I CCNGG 2 cut(s) 273, 317
MwoI GCNNNNNNNGC 1 cut(s) 166
NciI CCSGG 2 cut(s) 273, 317
NdeII GATC 4 cut(s) 74, 121, 145, 357
NlaIII CATG 2 cut(s) 94, 254
NlaIV GGNNCC 1 cut(s) 76
NmuCI GTSAC 1 cut(s) 328
PkrI GCNGC 1 cut(s) 84
PleI GAGTC 2 cut(s) 270, 454
PpsI GAGTC 2 cut(s) 270, 454
PsiI TTATAA 1 cut(s) 257
PspN4I GGNNCC 1 cut(s) 76
PspPI GGNCC 1 cut(s) 94
PsuI RGATCY 2 cut(s) 74, 145
SaqAI TTAA 1 cut(s) 429
SatI GCNGC 1 cut(s) 83
Sau3AI GATC 4 cut(s) 74, 121, 145, 357
Sau96I GGNCC 1 cut(s) 94
SchI GAGTC 2 cut(s) 270, 454
ScrFI CCNGG 2 cut(s) 273, 317
SduI GDGCHC 1 cut(s) 395
SetI ASST 9 cut(s) 16, 38, 65, 171, 294, 306, 372, 380, 407
SinI GGWCC 1 cut(s) 94
SmlI CTYRAG 1 cut(s) 379
SmoI CTYRAG 1 cut(s) 379
SpeI ACTAGT 1 cut(s) 107
Sse9I AATT 1 cut(s) 464
SspI AATATT 1 cut(s) 25
SspMI CTAG 3 cut(s) 60, 108, 161
StyD4I CCNGG 2 cut(s) 271, 315
TaqI TCGA 2 cut(s) 120, 300
TaqII GACCGA 1 cut(s) 111
TasI AATT 1 cut(s) 464
Tru1I TTAA 1 cut(s) 429
Tru9I TTAA 1 cut(s) 429
TseFI GTSAC 1 cut(s) 328
TseI GCWGC 1 cut(s) 82
Tsp45I GTSAC 1 cut(s) 328
TspDTI ATGAA 2 cut(s) 79, 458
TspGWI ACGGA 1 cut(s) 138
VpaK11BI GGWCC 1 cut(s) 94
XspI CTAG 3 cut(s) 60, 108, 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.