MD05G1183400.v1.1

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0020267
Physical Location & Seq
Reverse (-)
31196823 .. 31197443
621 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1183400.v1.1.491

Sequence Viewer

Length: 621 bp
ATGAGGAAACAACCTTCTCTTAATATTGTCATCACCTTGTGCCTTCTCCTTTTTCTTTTTAGGTCATCCAATGGGTCCTTGCTGCCGTTCATGGATCGACCAGGAACTAGTTCTCTGGTCGATCTCTTGCCGGAGCACTTTCCAGATCCGTTCCGAGTGCTAGAACAGCTCCCATTTGAACTTGGAAAAGAAGAGAGCCTGCTGGCACTCTCACCAGCAAAAGTGGACTGGAAAGAAACGCCGGAGGGTCATGTTATAATGTTAGATGTGCCAGGACTCAACAAGGAGGAGCTGAAAATCGAGGTTGAGGAGAACCGGGTTCTGAGAGTGAGTGGAGAGAGGAAGAGGGAAGAAGACAAGAAAGGGGATCACTGGCACCGAGTTGAGAGGTCTTACGGAAAATTTTGGAGGCGGTTTAAGTTGCCTGAGAACGTGGATTTAGACTCCATCAACGCCAAGTTGGAAAATGGGGTTCTCACGGTGACGCTGGCCAAGTTGTCCCCTGATAAGATCAAGGCAGCTACTAGGGTGATTAGCATCTCTACCACTGGAGGAGGAGGAGGAGGAGATCAGGGTGAACCTGCCAAGCTCAAGAGTAGCGAGCCCAAGCAAGAGCTTTAA

Protein Analysis

207

Amino Acids

23.14

Weight (kDa)

6.46

Isoelectric Point (pI)

52.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 76 - 180 3.5e-31 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0020267)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10250
malus_domestica MD05G1183400.v1.1 MD10G1171200.v1.1
prunus_persica Prupe.8G000400_v2.0.a1
pyrus_communis pycom05g16840 pycom10g14760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 257
Acc36I ACCTGC 1 cut(s) 589
AccB1I GGYRCC 1 cut(s) 375
AciI CCGC 1 cut(s) 412
AclWI GGATC 3 cut(s) 102, 140, 375
AcoI YGGCCR 1 cut(s) 489
AcsI RAATTY 1 cut(s) 401
AdeI CACNNNGTG 1 cut(s) 39
AgsI TTSAA 1 cut(s) 179
AhlI ACTAGT 1 cut(s) 107
AjnI CCWGG 2 cut(s) 100, 271
AluBI AGCT 5 cut(s) 169, 292, 521, 589, 616
AluI AGCT 5 cut(s) 169, 292, 521, 589, 616
Alw21I GWGCWC 1 cut(s) 138
AlwI GGATC 3 cut(s) 102, 140, 375
AoxI GGCC 1 cut(s) 489
ApeKI GCWGC 2 cut(s) 82, 518
ApoI RAATTY 1 cut(s) 401
Asp700I GAANNNNTTC 1 cut(s) 109
AspS9I GGNCC 1 cut(s) 75
AsuC2I CCSGG 1 cut(s) 317
AsuHPI GGTGA 5 cut(s) 25, 204, 493, 541, 587
AvaII GGWCC 1 cut(s) 75
BalI TGGCCA 1 cut(s) 491
BanI GGYRCC 1 cut(s) 375
BanII GRGCYC 1 cut(s) 606
BbsI GAAGAC 1 cut(s) 360
Bbv12I GWGCWC 1 cut(s) 138
BbvI GCAGC 2 cut(s) 69, 530
BccI CCATC 1 cut(s) 455
BceAI ACGGC 1 cut(s) 70
BciT130I CCWGG 2 cut(s) 102, 273
BcnI CCSGG 1 cut(s) 317
BcuI ACTAGT 1 cut(s) 107
BfaI CTAG 3 cut(s) 108, 161, 525
BfuAI ACCTGC 1 cut(s) 589
BisI GCNGC 2 cut(s) 83, 519
BlsI GCNGC 2 cut(s) 84, 520
Bme1390I CCNGG 3 cut(s) 102, 273, 317
Bme18I GGWCC 1 cut(s) 75
BmgT120I GGNCC 1 cut(s) 75
BmiI GGNNCC 2 cut(s) 76, 377
BmrFI CCNGG 3 cut(s) 102, 273, 317
BmsI GCATC 1 cut(s) 546
BpiI GAAGAC 1 cut(s) 360
BpmI CTGGAG 1 cut(s) 570
BpuEI CTTGAG 1 cut(s) 575
BpuMI CCSGG 1 cut(s) 317
BsaBI GATNNNNATC 1 cut(s) 536
Bse1I ACTGG 3 cut(s) 233, 377, 553
Bse8I GATNNNNATC 1 cut(s) 536
BseBI CCWGG 2 cut(s) 102, 273
BseGI GGATG 1 cut(s) 65
BseJI GATNNNNATC 1 cut(s) 536
BseMII CTCAG 2 cut(s) 314, 417
BseNI ACTGG 3 cut(s) 233, 377, 553
BseRI GAGGAG 7 cut(s) 302, 323, 567, 570, 573, 576, 579
BseXI GCAGC 2 cut(s) 69, 530
BshFI GGCC 1 cut(s) 491
BshNI GGYRCC 1 cut(s) 375
BsiHKAI GWGCWC 1 cut(s) 138
BsiSI CCGG 3 cut(s) 131, 242, 316
BslFI GGGAC 1 cut(s) 484
BsmFI GGGAC 1 cut(s) 484
BsnI GGCC 1 cut(s) 491
Bsp1286I GDGCHC 2 cut(s) 138, 606
Bsp143I GATC 6 cut(s) 94, 121, 145, 367, 510, 568
BspACI CCGC 1 cut(s) 412
BspANI GGCC 1 cut(s) 491
BspCNI CTCAG 2 cut(s) 315, 418
BspLI GGNNCC 2 cut(s) 76, 377
BspMI ACCTGC 1 cut(s) 589
BspPI GGATC 3 cut(s) 102, 140, 375
BspT107I GGYRCC 1 cut(s) 375
BsrI ACTGG 3 cut(s) 233, 377, 553
BssMI GATC 6 cut(s) 94, 121, 145, 367, 510, 568
Bst2UI CCWGG 2 cut(s) 102, 273
Bst4CI ACNGT 1 cut(s) 481
Bst6I CTCTTC 2 cut(s) 186, 338
BstC8I GCNNGC 4 cut(s) 200, 204, 489, 602
BstDEI CTNAG 2 cut(s) 323, 426
BstF5I GGATG 1 cut(s) 65
BstKTI GATC 6 cut(s) 97, 124, 148, 370, 513, 571
BstMBI GATC 6 cut(s) 94, 121, 145, 367, 510, 568
BstMWI GCNNNNNNNGC 1 cut(s) 166
BstNI CCWGG 2 cut(s) 102, 273
BstSCI CCNGG 3 cut(s) 100, 271, 315
BstV1I GCAGC 2 cut(s) 69, 530
BstV2I GAAGAC 1 cut(s) 360
BstX2I RGATCY 1 cut(s) 145
BstYI RGATCY 1 cut(s) 145
BsuRI GGCC 1 cut(s) 491
BtsCI GGATG 1 cut(s) 65
BtsIMutI CAGTG 2 cut(s) 370, 546
BveI ACCTGC 1 cut(s) 589
Cac8I GCNNGC 4 cut(s) 200, 204, 489, 602
Cfr13I GGNCC 1 cut(s) 75
CseI GACGC 1 cut(s) 493
CviAII CATG 2 cut(s) 91, 251
CviJI RGCY 8 cut(s) 169, 198, 292, 491, 521, 589, 604, 616
CviKI_1 RGCY 8 cut(s) 169, 198, 292, 491, 521, 589, 604, 616
DdeI CTNAG 2 cut(s) 323, 426
DpnI GATC 6 cut(s) 96, 123, 147, 369, 512, 570
DpnII GATC 6 cut(s) 94, 121, 145, 367, 510, 568
DraIII CACNNNGTG 1 cut(s) 39
EaeI YGGCCR 1 cut(s) 489
Eam1104I CTCTTC 2 cut(s) 186, 338
EarI CTCTTC 2 cut(s) 186, 338
Eco24I GRGCYC 1 cut(s) 606
Eco47I GGWCC 1 cut(s) 75
EcoO109I RGGNCCY 1 cut(s) 75
EcoRII CCWGG 2 cut(s) 100, 271
EcoT38I GRGCYC 1 cut(s) 606
FaeI CATG 2 cut(s) 94, 254
FaiI YATR 3 cut(s) 92, 252, 257
FaqI GGGAC 1 cut(s) 484
FatI CATG 2 cut(s) 90, 250
Fnu4HI GCNGC 2 cut(s) 83, 519
FokI GGATG 1 cut(s) 52
FriOI GRGCYC 1 cut(s) 606
Fsp4HI GCNGC 2 cut(s) 83, 519
FspBI CTAG 3 cut(s) 108, 161, 525
GluI GCNGC 2 cut(s) 83, 519
GsuI CTGGAG 1 cut(s) 570
HaeIII GGCC 1 cut(s) 491
HapII CCGG 3 cut(s) 131, 242, 316
HgaI GACGC 1 cut(s) 493
Hin1II CATG 2 cut(s) 94, 254
HinfI GANTC 2 cut(s) 276, 443
HpaII CCGG 3 cut(s) 131, 242, 316
HphI GGTGA 5 cut(s) 25, 204, 493, 541, 587
Hpy166II GTNNAC 2 cut(s) 226, 578
Hpy188I TCNGA 2 cut(s) 155, 324
Hpy188III TCNNGA 2 cut(s) 143, 592
Hpy8I GTNNAC 2 cut(s) 226, 578
HpyAV CCTTC 2 cut(s) 24, 53
HpyCH4III ACNGT 1 cut(s) 481
HpyCH4IV ACGT 1 cut(s) 432
HpyF10VI GCNNNNNNNGC 1 cut(s) 166
HpyF3I CTNAG 2 cut(s) 323, 426
HpySE526I ACGT 1 cut(s) 432
Hsp92II CATG 2 cut(s) 94, 254
Kzo9I GATC 6 cut(s) 94, 121, 145, 367, 510, 568
LmnI GCTCC 3 cut(s) 133, 174, 289
Lsp1109I GCAGC 2 cut(s) 69, 530
LweI GCATC 1 cut(s) 546
MaeI CTAG 3 cut(s) 108, 161, 525
MaeII ACGT 1 cut(s) 432
MaeIII GTNAC 1 cut(s) 481
MalI GATC 6 cut(s) 96, 123, 147, 369, 512, 570
MboI GATC 6 cut(s) 94, 121, 145, 367, 510, 568
MboII GAAGA 4 cut(s) 203, 355, 362, 365
MflI RGATCY 1 cut(s) 145
MhlI GDGCHC 2 cut(s) 138, 606
MlsI TGGCCA 1 cut(s) 491
MluCI AATT 1 cut(s) 401
MluNI TGGCCA 1 cut(s) 491
MlyI GAGTC 2 cut(s) 270, 437
MmeI TCCRAC 1 cut(s) 441
Mox20I TGGCCA 1 cut(s) 491
MroXI GAANNNNTTC 1 cut(s) 109
MscI TGGCCA 1 cut(s) 491
MseI TTAA 3 cut(s) 21, 417, 619
Msp20I TGGCCA 1 cut(s) 491
MspI CCGG 3 cut(s) 131, 242, 316
MspR9I CCNGG 3 cut(s) 102, 273, 317
MvaI CCWGG 2 cut(s) 102, 273
MwoI GCNNNNNNNGC 1 cut(s) 166
NciI CCSGG 1 cut(s) 317
NdeII GATC 6 cut(s) 94, 121, 145, 367, 510, 568
NlaIII CATG 2 cut(s) 94, 254
NlaIV GGNNCC 2 cut(s) 76, 377
NmuCI GTSAC 1 cut(s) 481
PdmI GAANNNNTTC 1 cut(s) 109
PkrI GCNGC 2 cut(s) 84, 520
PleI GAGTC 2 cut(s) 270, 437
PpsI GAGTC 2 cut(s) 270, 437
PpuMI RGGWCCY 1 cut(s) 75
PsiI TTATAA 1 cut(s) 257
Psp5II RGGWCCY 1 cut(s) 75
Psp6I CCWGG 2 cut(s) 100, 271
PspGI CCWGG 2 cut(s) 100, 271
PspN4I GGNNCC 2 cut(s) 76, 377
PspPI GGNCC 1 cut(s) 75
PspPPI RGGWCCY 1 cut(s) 75
PsuI RGATCY 1 cut(s) 145
SaqAI TTAA 3 cut(s) 21, 417, 619
SatI GCNGC 2 cut(s) 83, 519
Sau3AI GATC 6 cut(s) 94, 121, 145, 367, 510, 568
Sau96I GGNCC 1 cut(s) 75
SchI GAGTC 2 cut(s) 270, 437
ScrFI CCNGG 3 cut(s) 102, 273, 317
SduI GDGCHC 2 cut(s) 138, 606
SfaNI GCATC 1 cut(s) 546
SinI GGWCC 1 cut(s) 75
SmlI CTYRAG 1 cut(s) 590
SmoI CTYRAG 1 cut(s) 590
SpeI ACTAGT 1 cut(s) 107
Sse9I AATT 1 cut(s) 401
SsiI CCGC 1 cut(s) 412
SspI AATATT 1 cut(s) 25
SspMI CTAG 3 cut(s) 108, 161, 525
StyD4I CCNGG 3 cut(s) 100, 271, 315
TaaI ACNGT 1 cut(s) 481
TaiI ACGT 1 cut(s) 435
TaqI TCGA 3 cut(s) 97, 120, 300
TasI AATT 1 cut(s) 401
Tru1I TTAA 3 cut(s) 21, 417, 619
Tru9I TTAA 3 cut(s) 21, 417, 619
TscAI CASTG 2 cut(s) 377, 553
TseFI GTSAC 1 cut(s) 481
TseI GCWGC 2 cut(s) 82, 518
Tsp45I GTSAC 1 cut(s) 481
TspDTI ATGAA 1 cut(s) 79
TspGWI ACGGA 2 cut(s) 138, 411
TspRI CASTG 2 cut(s) 377, 553
VpaK11BI GGWCC 1 cut(s) 75
XapI RAATTY 1 cut(s) 401
XmnI GAANNNNTTC 1 cut(s) 109
XspI CTAG 3 cut(s) 108, 161, 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.