Prupe.8G000400_v2.0.a1

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0020267
Physical Location & Seq
Forward (+)
79404 .. 80031
628 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G000400.1

Sequence Viewer

Length: 603 bp
ATGAGGCAACTCCGCTCTCTTGTTTTAACCTTGAGCTTTCTCCTTGTTTTTCTTTCAGCTGGGTCATCCAATGCGTCCTTGCTGCCATTCGTTGATCGCCGCCCAGAAAGTTTGCTGGTTGATCTCTGGTCGGAGCGCTTCCCAGACCCGTTCCGAGTGCTAGAGCAGATCCCATTTGAACTTGACAAAGAAGACGGCGTGGCACTCTCTCCAGCAAGGGTGGACTGGAAAGAAACGCCAGAGGGTCATGTTATAATGTTAGATGTGCCGGGATTAAAGAAGGAAGAGCTGAAAATCGAGGTGGAGGCGAACCGAGTTCTGAGAGTGAGTGGAGAGAGGAAGAGGGAAGAAGAGAAGAAAGGGGATCATTGGCACCGAGTTGAGAGGTCTTACGGAAAATTTTGGAGGCAGTTCAGGCTGCCAGAGAACGTGGATTTGGACTCTGTCAACGCCAAGTTGGAGAATGGGGTTCTCACTTTGACACTGGCCAAGTTATCCCCTGATAAGATCAAAGGTCCTAAGGTGATTAGCATCGCTAGCACTGGAGGACATGAGGACGAACCTGCCCAGCTCAAGGCTAGTGAGGTCAAGCAAGAGCTTTAA

Protein Analysis

201

Amino Acids

22.77

Weight (kDa)

5.91

Isoelectric Point (pI)

46.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020267)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10250
malus_domestica MD05G1183400.v1.1 MD10G1171200.v1.1
prunus_persica Prupe.8G000400_v2.0.a1
pyrus_communis pycom05g16840 pycom10g14760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 254
Acc36I ACCTGC 1 cut(s) 571
AccB1I GGYRCC 1 cut(s) 372
AccBSI CCGCTC 1 cut(s) 15
AciI CCGC 2 cut(s) 13, 100
AclWI GGATC 2 cut(s) 163, 372
AcoI YGGCCR 1 cut(s) 486
AcsI RAATTY 1 cut(s) 398
AfeI AGCGCT 1 cut(s) 137
AfiI CCNNNNNNNGG 1 cut(s) 574
AgsI TTSAA 1 cut(s) 179
AjuI GAANNNNNNNTTGG 2 cut(s) 419, 451
AluBI AGCT 5 cut(s) 36, 59, 289, 571, 598
AluI AGCT 5 cut(s) 36, 59, 289, 571, 598
AlwI GGATC 2 cut(s) 163, 372
Aor51HI AGCGCT 1 cut(s) 137
AoxI GGCC 1 cut(s) 486
ApeKI GCWGC 2 cut(s) 82, 418
ApoI RAATTY 1 cut(s) 398
AspLEI GCGC 1 cut(s) 138
AspS9I GGNCC 1 cut(s) 515
AsuC2I CCSGG 1 cut(s) 270
AsuHPI GGTGA 1 cut(s) 535
AsuNHI GCTAGC 1 cut(s) 536
AvaII GGWCC 1 cut(s) 515
AxyI CCTNAGG 1 cut(s) 519
BalI TGGCCA 1 cut(s) 488
BanI GGYRCC 1 cut(s) 372
BbsI GAAGAC 1 cut(s) 198
BbvI GCAGC 2 cut(s) 69, 405
BceAI ACGGC 1 cut(s) 211
BcnI CCSGG 1 cut(s) 270
BfaI CTAG 3 cut(s) 161, 537, 579
BfoI RGCGCY 1 cut(s) 139
BfuAI ACCTGC 1 cut(s) 571
BisI GCNGC 3 cut(s) 83, 100, 419
BlsI GCNGC 3 cut(s) 84, 101, 420
Bme1390I CCNGG 1 cut(s) 270
Bme18I GGWCC 1 cut(s) 515
BmgT120I GGNCC 1 cut(s) 515
BmiI GGNNCC 1 cut(s) 374
BmrFI CCNGG 1 cut(s) 270
BmsI GCATC 1 cut(s) 540
BmtI GCTAGC 1 cut(s) 540
BpiI GAAGAC 1 cut(s) 198
BpmI CTGGAG 2 cut(s) 195, 564
BpuEI CTTGAG 2 cut(s) 52, 557
BpuMI CCSGG 1 cut(s) 270
BsaBI GATNNNNATC 1 cut(s) 530
Bsc4I CCNNNNNNNGG 1 cut(s) 574
Bse1I ACTGG 3 cut(s) 230, 489, 547
Bse21I CCTNAGG 1 cut(s) 519
Bse8I GATNNNNATC 1 cut(s) 530
BseGI GGATG 1 cut(s) 65
BseJI GATNNNNATC 1 cut(s) 530
BseLI CCNNNNNNNGG 1 cut(s) 574
BseMII CTCAG 1 cut(s) 311
BseNI ACTGG 3 cut(s) 230, 489, 547
BseXI GCAGC 2 cut(s) 69, 405
BseYI CCCAGC 2 cut(s) 59, 567
BshFI GGCC 1 cut(s) 488
BshNI GGYRCC 1 cut(s) 372
BsiSI CCGG 1 cut(s) 269
BslI CCNNNNNNNGG 1 cut(s) 574
BsnI GGCC 1 cut(s) 488
Bsp143I GATC 5 cut(s) 94, 121, 168, 364, 507
BspACI CCGC 2 cut(s) 13, 100
BspANI GGCC 1 cut(s) 488
BspCNI CTCAG 1 cut(s) 312
BspLI GGNNCC 1 cut(s) 374
BspMI ACCTGC 1 cut(s) 571
BspOI GCTAGC 1 cut(s) 540
BspPI GGATC 2 cut(s) 163, 372
BspQI GCTCTTC 1 cut(s) 279
BspT107I GGYRCC 1 cut(s) 372
BsrBI CCGCTC 1 cut(s) 15
BsrI ACTGG 3 cut(s) 230, 489, 547
BssMI GATC 5 cut(s) 94, 121, 168, 364, 507
Bst6I CTCTTC 3 cut(s) 279, 335, 345
BstC8I GCNNGC 1 cut(s) 538
BstDEI CTNAG 2 cut(s) 320, 519
BstF5I GGATG 1 cut(s) 65
BstH2I RGCGCY 1 cut(s) 139
BstHHI GCGC 1 cut(s) 138
BstKTI GATC 5 cut(s) 97, 124, 171, 367, 510
BstMBI GATC 5 cut(s) 94, 121, 168, 364, 507
BstMWI GCNNNNNNNGC 2 cut(s) 415, 537
BstSCI CCNGG 1 cut(s) 268
BstV1I GCAGC 2 cut(s) 69, 405
BstV2I GAAGAC 1 cut(s) 198
BstX2I RGATCY 1 cut(s) 168
BstYI RGATCY 1 cut(s) 168
Bsu36I CCTNAGG 1 cut(s) 519
BsuRI GGCC 1 cut(s) 488
BtgZI GCGATG 1 cut(s) 517
BtsCI GGATG 1 cut(s) 65
BtsIMutI CAGTG 2 cut(s) 482, 540
BveI ACCTGC 1 cut(s) 571
Cac8I GCNNGC 1 cut(s) 538
CfoI GCGC 1 cut(s) 138
Cfr13I GGNCC 1 cut(s) 515
CseI GACGC 1 cut(s) 63
CviAII CATG 2 cut(s) 248, 551
CviJI RGCY 8 cut(s) 36, 59, 289, 418, 488, 571, 578, 598
CviKI_1 RGCY 8 cut(s) 36, 59, 289, 418, 488, 571, 578, 598
DdeI CTNAG 2 cut(s) 320, 519
DpnI GATC 5 cut(s) 96, 123, 170, 366, 509
DpnII GATC 5 cut(s) 94, 121, 168, 364, 507
EaeI YGGCCR 1 cut(s) 486
Eam1104I CTCTTC 3 cut(s) 279, 335, 345
EarI CTCTTC 3 cut(s) 279, 335, 345
Eco47I GGWCC 1 cut(s) 515
Eco47III AGCGCT 1 cut(s) 137
Eco81I CCTNAGG 1 cut(s) 519
EcoO109I RGGNCCY 1 cut(s) 515
FaeI CATG 2 cut(s) 251, 554
FaiI YATR 3 cut(s) 249, 254, 552
FatI CATG 2 cut(s) 247, 550
Fnu4HI GCNGC 3 cut(s) 83, 100, 419
FokI GGATG 1 cut(s) 52
Fsp4HI GCNGC 3 cut(s) 83, 100, 419
FspBI CTAG 3 cut(s) 161, 537, 579
GlaI GCGC 1 cut(s) 137
GluI GCNGC 3 cut(s) 83, 100, 419
GsaI CCCAGC 2 cut(s) 63, 571
GsuI CTGGAG 2 cut(s) 195, 564
HaeII RGCGCY 1 cut(s) 139
HaeIII GGCC 1 cut(s) 488
HapII CCGG 1 cut(s) 269
HgaI GACGC 1 cut(s) 63
HhaI GCGC 1 cut(s) 138
Hin1II CATG 2 cut(s) 251, 554
Hin6I GCGC 1 cut(s) 136
HinP1I GCGC 1 cut(s) 136
HincII GTYRAC 1 cut(s) 448
HindII GTYRAC 1 cut(s) 448
HinfI GANTC 1 cut(s) 440
HpaII CCGG 1 cut(s) 269
HphI GGTGA 1 cut(s) 535
Hpy166II GTNNAC 2 cut(s) 223, 448
Hpy188I TCNGA 3 cut(s) 133, 155, 321
Hpy8I GTNNAC 2 cut(s) 223, 448
HpyAV CCTTC 1 cut(s) 274
HpyCH4IV ACGT 1 cut(s) 429
HpyF10VI GCNNNNNNNGC 2 cut(s) 415, 537
HpyF3I CTNAG 2 cut(s) 320, 519
HpySE526I ACGT 1 cut(s) 429
Hsp92II CATG 2 cut(s) 251, 554
HspAI GCGC 1 cut(s) 136
Kzo9I GATC 5 cut(s) 94, 121, 168, 364, 507
LguI GCTCTTC 1 cut(s) 279
LmnI GCTCC 1 cut(s) 133
Lsp1109I GCAGC 2 cut(s) 69, 405
LweI GCATC 1 cut(s) 540
MaeI CTAG 3 cut(s) 161, 537, 579
MaeII ACGT 1 cut(s) 429
MalI GATC 5 cut(s) 96, 123, 170, 366, 509
MbiI CCGCTC 1 cut(s) 15
MboI GATC 5 cut(s) 94, 121, 168, 364, 507
MboII GAAGA 6 cut(s) 203, 296, 352, 359, 362, 367
MflI RGATCY 1 cut(s) 168
MlsI TGGCCA 1 cut(s) 488
MluCI AATT 1 cut(s) 398
MluNI TGGCCA 1 cut(s) 488
MlyI GAGTC 1 cut(s) 434
MmeI TCCRAC 2 cut(s) 111, 438
Mox20I TGGCCA 1 cut(s) 488
MscI TGGCCA 1 cut(s) 488
MseI TTAA 3 cut(s) 26, 275, 601
Msp20I TGGCCA 1 cut(s) 488
MspA1I CMGCKG 1 cut(s) 59
MspI CCGG 1 cut(s) 269
MspR9I CCNGG 1 cut(s) 270
MwoI GCNNNNNNNGC 2 cut(s) 415, 537
NciI CCSGG 1 cut(s) 270
NdeII GATC 5 cut(s) 94, 121, 168, 364, 507
NheI GCTAGC 1 cut(s) 536
NlaIII CATG 2 cut(s) 251, 554
NlaIV GGNNCC 1 cut(s) 374
PciSI GCTCTTC 1 cut(s) 279
PflFI GACNNNGTC 1 cut(s) 443
PkrI GCNGC 3 cut(s) 84, 101, 420
PleI GAGTC 1 cut(s) 434
PpsI GAGTC 1 cut(s) 434
PpuMI RGGWCCY 1 cut(s) 515
PsiI TTATAA 1 cut(s) 254
Psp5II RGGWCCY 1 cut(s) 515
PspFI CCCAGC 2 cut(s) 59, 567
PspN4I GGNNCC 1 cut(s) 374
PspPI GGNCC 1 cut(s) 515
PspPPI RGGWCCY 1 cut(s) 515
PsuI RGATCY 1 cut(s) 168
PsyI GACNNNGTC 1 cut(s) 443
PvuII CAGCTG 1 cut(s) 59
SapI GCTCTTC 1 cut(s) 279
SaqAI TTAA 3 cut(s) 26, 275, 601
SatI GCNGC 3 cut(s) 83, 100, 419
Sau3AI GATC 5 cut(s) 94, 121, 168, 364, 507
Sau96I GGNCC 1 cut(s) 515
SchI GAGTC 1 cut(s) 434
ScrFI CCNGG 1 cut(s) 270
SfaNI GCATC 1 cut(s) 540
SinI GGWCC 1 cut(s) 515
SmlI CTYRAG 2 cut(s) 31, 572
SmoI CTYRAG 2 cut(s) 31, 572
Sse9I AATT 1 cut(s) 398
SsiI CCGC 2 cut(s) 13, 100
SspMI CTAG 3 cut(s) 161, 537, 579
StyD4I CCNGG 1 cut(s) 268
TaiI ACGT 1 cut(s) 432
TaqI TCGA 1 cut(s) 297
TasI AATT 1 cut(s) 398
TauI GCSGC 1 cut(s) 102
Tru1I TTAA 3 cut(s) 26, 275, 601
Tru9I TTAA 3 cut(s) 26, 275, 601
TscAI CASTG 2 cut(s) 489, 547
TseI GCWGC 2 cut(s) 82, 418
TspGWI ACGGA 1 cut(s) 408
TspRI CASTG 2 cut(s) 489, 547
Tth111I GACNNNGTC 1 cut(s) 443
VpaK11BI GGWCC 1 cut(s) 515
XapI RAATTY 1 cut(s) 398
XspI CTAG 3 cut(s) 161, 537, 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.