MD10G1171200.v1.1

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0020267
Physical Location & Seq
Reverse (-)
26409043 .. 26409660
618 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1171200.v1.1.491

Sequence Viewer

Length: 618 bp
ATGAGGAAACAACCTTGTCGAAATATTCTCATCGCCTTGAGCTTTCTGCTTTTTCTTTCTAGGTCATCCAATGGGTCCTTGCTGCCGTTCATGGATCGACCAGGAAGTAATTTTCTGGTCGATCTCTGGTCGGAGCGCTTTCCAGACCCCTTCCGCGTGCTAGAACAGCTCCCGTTTGAACTTGACAAAGAAGACACCCTGCTGGCACTCTCACCAGCAAAGGTGGACTGGAAAGAAACGCCGGACGGCCATGTTATAATGTTAGATGTGCCGGGACTCAAGAAGGAGGACCTGAAAATCGAGGTGGAGGAGAACCGGGTTCTGAGAGTGAGTGGTGAGAGGAAGAAGGAAGAAGACAAGAAAGGGGATCACTGGCACCGAGTTGAGAGGTCTTATGGAAAATTTTGGAGGCGGTTTAGGTTGCCGGAGAACGTAGATTTAGACTCCGTCGAGGCCAAGTTGGAAAATGGGGTTCTCACTGTGACGCTGGCCAAGTTGTCTCCTCATACAATCAAGGCTACTCCTAGGGTGATTAGCATCTCTAGCACTGGAGGAGGAGGAGGAGATCAGGACGAACCTGCCAAGCTCAAGAATAGCCAGCCCAAGCAAGAGCTGTAA

Protein Analysis

206

Amino Acids

23.37

Weight (kDa)

6.93

Isoelectric Point (pI)

48.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 76 - 180 9.1e-32 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0020267)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10250
malus_domestica MD05G1183400.v1.1 MD10G1171200.v1.1
prunus_persica Prupe.8G000400_v2.0.a1
pyrus_communis pycom05g16840 pycom10g14760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 257
Acc36I ACCTGC 1 cut(s) 586
AccB1I GGYRCC 1 cut(s) 375
AccII CGCG 1 cut(s) 156
AciI CCGC 2 cut(s) 154, 412
AclWI GGATC 2 cut(s) 102, 375
AcoI YGGCCR 2 cut(s) 247, 489
AcsI RAATTY 1 cut(s) 401
AfeI AGCGCT 1 cut(s) 137
AgsI TTSAA 1 cut(s) 179
AjnI CCWGG 1 cut(s) 100
AluBI AGCT 4 cut(s) 42, 169, 586, 613
AluI AGCT 4 cut(s) 42, 169, 586, 613
Alw26I GTCTC 1 cut(s) 504
AlwI GGATC 2 cut(s) 102, 375
Aor51HI AGCGCT 1 cut(s) 137
AoxI GGCC 3 cut(s) 247, 453, 489
ApeKI GCWGC 1 cut(s) 82
ApoI RAATTY 1 cut(s) 401
AspA2I CCTAGG 1 cut(s) 524
AspLEI GCGC 1 cut(s) 138
AspS9I GGNCC 2 cut(s) 75, 289
AsuC2I CCSGG 2 cut(s) 273, 317
AsuHPI GGTGA 3 cut(s) 204, 347, 541
AvaII GGWCC 2 cut(s) 75, 289
AvrII CCTAGG 1 cut(s) 524
BalI TGGCCA 1 cut(s) 491
BanI GGYRCC 1 cut(s) 375
BbsI GAAGAC 2 cut(s) 198, 360
BbvI GCAGC 1 cut(s) 69
BceAI ACGGC 2 cut(s) 70, 262
BciT130I CCWGG 1 cut(s) 102
BcnI CCSGG 2 cut(s) 273, 317
BcoDI GTCTC 1 cut(s) 504
BfaI CTAG 4 cut(s) 60, 161, 525, 543
BfoI RGCGCY 1 cut(s) 139
BfuAI ACCTGC 1 cut(s) 586
BisI GCNGC 1 cut(s) 83
BlnI CCTAGG 1 cut(s) 524
BlsI GCNGC 1 cut(s) 84
Bme1390I CCNGG 3 cut(s) 102, 273, 317
Bme18I GGWCC 2 cut(s) 75, 289
BmgT120I GGNCC 2 cut(s) 75, 289
BmiI GGNNCC 2 cut(s) 76, 377
BmrFI CCNGG 3 cut(s) 102, 273, 317
BmsI GCATC 1 cut(s) 546
BpiI GAAGAC 2 cut(s) 198, 360
BpmI CTGGAG 1 cut(s) 570
BpuEI CTTGAG 3 cut(s) 58, 263, 572
BpuMI CCSGG 2 cut(s) 273, 317
BsaBI GATNNNNATC 1 cut(s) 536
BsaJI CCNNGG 1 cut(s) 524
Bse1I ACTGG 3 cut(s) 233, 377, 553
Bse8I GATNNNNATC 1 cut(s) 536
BseBI CCWGG 1 cut(s) 102
BseDI CCNNGG 1 cut(s) 524
BseGI GGATG 1 cut(s) 65
BseJI GATNNNNATC 1 cut(s) 536
BseMII CTCAG 1 cut(s) 314
BseNI ACTGG 3 cut(s) 233, 377, 553
BseRI GAGGAG 6 cut(s) 323, 492, 567, 570, 573, 576
BseXI GCAGC 1 cut(s) 69
Bsh1236I CGCG 1 cut(s) 156
BshFI GGCC 3 cut(s) 249, 455, 491
BshNI GGYRCC 1 cut(s) 375
BsiSI CCGG 4 cut(s) 242, 272, 316, 425
BslFI GGGAC 1 cut(s) 288
BsmAI GTCTC 1 cut(s) 504
BsmFI GGGAC 1 cut(s) 288
BsnI GGCC 3 cut(s) 249, 455, 491
Bsp143I GATC 4 cut(s) 94, 121, 367, 565
BspACI CCGC 2 cut(s) 154, 412
BspANI GGCC 3 cut(s) 249, 455, 491
BspCNI CTCAG 1 cut(s) 315
BspFNI CGCG 1 cut(s) 156
BspLI GGNNCC 2 cut(s) 76, 377
BspMI ACCTGC 1 cut(s) 586
BspPI GGATC 2 cut(s) 102, 375
BspT107I GGYRCC 1 cut(s) 375
BsrI ACTGG 3 cut(s) 233, 377, 553
BssECI CCNNGG 1 cut(s) 524
BssMI GATC 4 cut(s) 94, 121, 367, 565
BssT1I CCWWGG 1 cut(s) 524
Bst2UI CCWGG 1 cut(s) 102
Bst4CI ACNGT 1 cut(s) 481
BstC8I GCNNGC 4 cut(s) 158, 204, 489, 599
BstDEI CTNAG 1 cut(s) 323
BstF5I GGATG 1 cut(s) 65
BstFNI CGCG 1 cut(s) 156
BstH2I RGCGCY 1 cut(s) 139
BstHHI GCGC 1 cut(s) 138
BstKTI GATC 4 cut(s) 97, 124, 370, 568
BstMAI GTCTC 1 cut(s) 504
BstMBI GATC 4 cut(s) 94, 121, 367, 565
BstMWI GCNNNNNNNGC 2 cut(s) 166, 543
BstNI CCWGG 1 cut(s) 102
BstSCI CCNGG 3 cut(s) 100, 271, 315
BstUI CGCG 1 cut(s) 156
BstV1I GCAGC 1 cut(s) 69
BstV2I GAAGAC 2 cut(s) 198, 360
BsuRI GGCC 3 cut(s) 249, 455, 491
BtgZI GCGATG 1 cut(s) 16
BtsCI GGATG 1 cut(s) 65
BtsIMutI CAGTG 3 cut(s) 370, 477, 546
BveI ACCTGC 1 cut(s) 586
Cac8I GCNNGC 4 cut(s) 158, 204, 489, 599
CfoI GCGC 1 cut(s) 138
Cfr13I GGNCC 2 cut(s) 75, 289
CseI GACGC 1 cut(s) 493
CviAII CATG 2 cut(s) 91, 251
DdeI CTNAG 1 cut(s) 323
DpnI GATC 4 cut(s) 96, 123, 369, 567
DpnII GATC 4 cut(s) 94, 121, 367, 565
EaeI YGGCCR 2 cut(s) 247, 489
Eco130I CCWWGG 1 cut(s) 524
Eco47I GGWCC 2 cut(s) 75, 289
Eco47III AGCGCT 1 cut(s) 137
EcoO109I RGGNCCY 2 cut(s) 75, 289
EcoRII CCWGG 1 cut(s) 100
EcoT14I CCWWGG 1 cut(s) 524
ErhI CCWWGG 1 cut(s) 524
FaeI CATG 2 cut(s) 94, 254
FaiI YATR 5 cut(s) 92, 252, 257, 396, 507
FaqI GGGAC 1 cut(s) 288
FatI CATG 2 cut(s) 90, 250
Fnu4HI GCNGC 1 cut(s) 83
FokI GGATG 1 cut(s) 52
Fsp4HI GCNGC 1 cut(s) 83
FspBI CTAG 4 cut(s) 60, 161, 525, 543
GlaI GCGC 1 cut(s) 137
GluI GCNGC 1 cut(s) 83
GsuI CTGGAG 1 cut(s) 570
HaeII RGCGCY 1 cut(s) 139
HaeIII GGCC 3 cut(s) 249, 455, 491
HapII CCGG 4 cut(s) 242, 272, 316, 425
HgaI GACGC 1 cut(s) 493
HhaI GCGC 1 cut(s) 138
Hin1II CATG 2 cut(s) 94, 254
Hin6I GCGC 1 cut(s) 136
HinP1I GCGC 1 cut(s) 136
HinfI GANTC 2 cut(s) 276, 443
HpaII CCGG 4 cut(s) 242, 272, 316, 425
HphI GGTGA 3 cut(s) 204, 347, 541
Hpy166II GTNNAC 1 cut(s) 226
Hpy188I TCNGA 2 cut(s) 133, 324
Hpy188III TCNNGA 4 cut(s) 143, 280, 569, 589
Hpy8I GTNNAC 1 cut(s) 226
Hpy99I CGWCG 1 cut(s) 452
HpyAV CCTTC 3 cut(s) 160, 277, 340
HpyCH4III ACNGT 1 cut(s) 481
HpyCH4IV ACGT 1 cut(s) 432
HpyF10VI GCNNNNNNNGC 2 cut(s) 166, 543
HpyF3I CTNAG 1 cut(s) 323
HpySE526I ACGT 1 cut(s) 432
Hsp92II CATG 2 cut(s) 94, 254
HspAI GCGC 1 cut(s) 136
Kzo9I GATC 4 cut(s) 94, 121, 367, 565
LmnI GCTCC 2 cut(s) 133, 174
Lsp1109I GCAGC 1 cut(s) 69
LweI GCATC 1 cut(s) 546
MaeI CTAG 4 cut(s) 60, 161, 525, 543
MaeII ACGT 1 cut(s) 432
MaeIII GTNAC 1 cut(s) 481
MalI GATC 4 cut(s) 96, 123, 369, 567
MboI GATC 4 cut(s) 94, 121, 367, 565
MboII GAAGA 4 cut(s) 203, 355, 362, 365
MlsI TGGCCA 1 cut(s) 491
MluCI AATT 2 cut(s) 109, 401
MluNI TGGCCA 1 cut(s) 491
MlyI GAGTC 2 cut(s) 270, 437
MmeI TCCRAC 2 cut(s) 111, 441
Mox20I TGGCCA 1 cut(s) 491
MscI TGGCCA 1 cut(s) 491
Msp20I TGGCCA 1 cut(s) 491
MspI CCGG 4 cut(s) 242, 272, 316, 425
MspR9I CCNGG 3 cut(s) 102, 273, 317
MvaI CCWGG 1 cut(s) 102
MvnI CGCG 1 cut(s) 156
MwoI GCNNNNNNNGC 2 cut(s) 166, 543
NciI CCSGG 2 cut(s) 273, 317
NdeII GATC 4 cut(s) 94, 121, 367, 565
NlaIII CATG 2 cut(s) 94, 254
NlaIV GGNNCC 2 cut(s) 76, 377
NmuCI GTSAC 1 cut(s) 481
PflFI GACNNNGTC 1 cut(s) 446
PkrI GCNGC 1 cut(s) 84
PleI GAGTC 2 cut(s) 270, 437
PpsI GAGTC 2 cut(s) 270, 437
PpuMI RGGWCCY 2 cut(s) 75, 289
PsiI TTATAA 1 cut(s) 257
Psp5II RGGWCCY 2 cut(s) 75, 289
Psp6I CCWGG 1 cut(s) 100
PspGI CCWGG 1 cut(s) 100
PspN4I GGNNCC 2 cut(s) 76, 377
PspPI GGNCC 2 cut(s) 75, 289
PspPPI RGGWCCY 2 cut(s) 75, 289
PsyI GACNNNGTC 1 cut(s) 446
SatI GCNGC 1 cut(s) 83
Sau3AI GATC 4 cut(s) 94, 121, 367, 565
Sau96I GGNCC 2 cut(s) 75, 289
SchI GAGTC 2 cut(s) 270, 437
ScrFI CCNGG 3 cut(s) 102, 273, 317
SfaNI GCATC 1 cut(s) 546
SinI GGWCC 2 cut(s) 75, 289
SmlI CTYRAG 3 cut(s) 37, 278, 587
SmoI CTYRAG 3 cut(s) 37, 278, 587
Sse9I AATT 2 cut(s) 109, 401
SsiI CCGC 2 cut(s) 154, 412
SspI AATATT 1 cut(s) 25
SspMI CTAG 4 cut(s) 60, 161, 525, 543
StyD4I CCNGG 3 cut(s) 100, 271, 315
StyI CCWWGG 1 cut(s) 524
TaaI ACNGT 1 cut(s) 481
TaiI ACGT 1 cut(s) 435
TaqI TCGA 5 cut(s) 19, 97, 120, 300, 450
TasI AATT 2 cut(s) 109, 401
TscAI CASTG 3 cut(s) 377, 484, 553
TseFI GTSAC 1 cut(s) 481
TseI GCWGC 1 cut(s) 82
Tsp45I GTSAC 1 cut(s) 481
TspDTI ATGAA 1 cut(s) 79
TspGWI ACGGA 1 cut(s) 436
TspRI CASTG 3 cut(s) 377, 484, 553
Tth111I GACNNNGTC 1 cut(s) 446
VpaK11BI GGWCC 2 cut(s) 75, 289
XapI RAATTY 1 cut(s) 401
XmaJI CCTAGG 1 cut(s) 524
XspI CTAG 4 cut(s) 60, 161, 525, 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.