AT4G10260

Belongs to the carbohydrate kinase PfkB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0012543
Physical Location & Seq
Reverse (-)
6371377 .. 6372789
1413 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G10260.1

Sequence Viewer

Length: 975 bp
ATGGCTAATACTCCATTGATAGTTTCCTTTGGTGAAATGCTTATCGACTTTGTCCCTGACACTTCTGGTGTTTCTTTAGCTGAGTCCACCGGTTTTCTCAAAGCCCCTGGTGGTGCTCCTGCTAATGTTGCTTGTGCCATCACCAAACTCGGTGGCAAATCAGCTTTCATCGGCAAGTTTGGTGATGATGAGTTTGGACACATGTTGGTTAACATATTGAAGAAGAACGGTGTGAACAGTGAAGGTGTTTGCTTTGATACCAATGCAAGAACCGCGTTGGCCTTTGTGACTTTGAAGAAAGATGGTGAGAGAGAGTTTATGTTTTACAGGAACCCTAGTGCGGACATGCTCTTGAAAGAATCTGAACTCAACAAGGATCTTATCAAGAAAGCTAAGATTTTCCACTATGGTTCCATTAGCTTGATCTCTGAGCCATGTAGAACAGCTCACATGGCTGCGATGAAAACCGCTAAAGATGCGGGAGTCTTACTCTCTTATGACCCGAACGTTCGGTTGCCTCTTTGGCCTTCTACTGAAGCTGCTATAGAAGGCATCAAAAGTATTTGGAATGAGGCTGATATTATCAAGGTAAGCGATGATGAGGTAACGTTTCTAACGCGTGGAGATGCGGAGAAGGATGATGTTGTTTTGTCTCTTATGCATGATAAGCTTAAGCTGCTTATTGTTACTGATGGAGAGAAAGGTTGCAGATACTACACAAAGAAATTCAAAGGGAGAGTGCCTGGATACGCTGTGAAAGCAGTTGACACAACTGGAGCTGGTGATTCATTTGTTGGTGCATTTCTTGTCTCATTGGGAAAAGATGGCTCCATACTCGATGATGAAGGGAAGCTGAAGGAAGCACTTGCCTTTGCAAATGCATGTGGAGCAGTGTGTACTACTCAGAAAGGAGCTATTCCTGCACTTCCCACTCCAGCTGATGCTCAAAAGCTCATGAAATCTAAGTCTAAATAG

Protein Analysis

324

Amino Acids

34.69

Weight (kDa)

6.9

Isoelectric Point (pI)

21.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PfkB PF00294 6 - 311 6.8e-76 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012543)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10260
fragaria_vesca FvH4_2g00080
malus_domestica MD17G1267100.v1.1
prunus_persica Prupe.8G000700_v2.0.a1
pyrus_communis pycom17g26620
rosa_chinensis RchiOBHm_Chr6g0249341
rosa_laevigata RLG00000015590
rosa_multiflora Rmu_co8334337.1_g000001 Rmu_sc0000278.1_g000022 Rmu_sc0006234.1_g000016
rosa_roxburghii Rroxscaffold_7G00217830
rosa_rugosa Rorug05G0490600.1 Rorug05G0490700.1 Rorug05G0490800.1
rosa_samantha Rh6AG001300 Rh6BG009000 Rh6CG000400 Rh6DG000300
rosa_wichuraiana Rw6G000190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 275, 619
AciI CCGC 5 cut(s) 273, 341, 468, 479, 629
AclI AACGTT 2 cut(s) 507, 608
AclWI GGATC 1 cut(s) 384
AcsI RAATTY 1 cut(s) 725
AcuI CTGAAG 2 cut(s) 555, 875
AfaI GTAC 1 cut(s) 898
AfiI CCNNNNNNNGG 1 cut(s) 340
AflII CTTAAG 1 cut(s) 671
AflIII ACRYGT 2 cut(s) 201, 617
AgeI ACCGGT 1 cut(s) 89
AgsI TTSAA 4 cut(s) 220, 295, 355, 730
AjnI CCWGG 2 cut(s) 106, 742
Alw21I GWGCWC 1 cut(s) 118
Alw26I GTCTC 2 cut(s) 657, 814
AlwI GGATC 1 cut(s) 384
AoxI GGCC 2 cut(s) 279, 524
ApeKI GCWGC 3 cut(s) 455, 539, 676
ApoI RAATTY 1 cut(s) 725
AsiGI ACCGGT 1 cut(s) 89
AsuHPI GGTGA 5 cut(s) 44, 133, 194, 317, 794
Bbv12I GWGCWC 1 cut(s) 118
BbvI GCAGC 3 cut(s) 442, 526, 663
BccI CCATC 4 cut(s) 146, 296, 686, 818
BciT130I CCWGG 2 cut(s) 108, 744
BciVI GTATCC 1 cut(s) 740
BcoDI GTCTC 2 cut(s) 657, 814
BfaI CTAG 1 cut(s) 336
BfmI CTRYAG 1 cut(s) 543
BfrI CTTAAG 1 cut(s) 671
BfuI GTATCC 1 cut(s) 740
BglI GCCNNNNNGGC 1 cut(s) 523
BisI GCNGC 3 cut(s) 456, 540, 677
BlsI GCNGC 3 cut(s) 457, 541, 678
Bme1390I CCNGG 2 cut(s) 108, 744
BmiI GGNNCC 3 cut(s) 332, 412, 829
BmrFI CCNGG 2 cut(s) 108, 744
BmsI GCATC 4 cut(s) 466, 561, 616, 931
BplI GAGNNNNNCTC 2 cut(s) 474, 506
BpmI CTGGAG 2 cut(s) 795, 918
BsaJI CCNNGG 1 cut(s) 106
BsaWI WCCGGW 1 cut(s) 89
Bsc4I CCNNNNNNNGG 1 cut(s) 340
Bse118I RCCGGY 1 cut(s) 89
Bse1I ACTGG 1 cut(s) 778
BseBI CCWGG 2 cut(s) 108, 744
BseDI CCNNGG 1 cut(s) 106
BseGI GGATG 1 cut(s) 643
BseLI CCNNNNNNNGG 1 cut(s) 340
BseMII CTCAG 3 cut(s) 72, 420, 917
BseNI ACTGG 1 cut(s) 778
BseXI GCAGC 3 cut(s) 442, 526, 663
BsgI GTGCAG 1 cut(s) 906
Bsh1236I CGCG 2 cut(s) 275, 619
BshFI GGCC 2 cut(s) 281, 526
BshTI ACCGGT 1 cut(s) 89
BsiHKAI GWGCWC 1 cut(s) 118
BsiSI CCGG 1 cut(s) 90
BslFI GGGAC 1 cut(s) 38
BslI CCNNNNNNNGG 1 cut(s) 340
BsmAI GTCTC 2 cut(s) 657, 814
BsmFI GGGAC 1 cut(s) 38
BsnI GGCC 2 cut(s) 281, 526
Bsp1286I GDGCHC 1 cut(s) 118
Bsp143I GATC 2 cut(s) 376, 423
BspACI CCGC 5 cut(s) 273, 341, 468, 479, 629
BspANI GGCC 2 cut(s) 281, 526
BspCNI CTCAG 3 cut(s) 73, 421, 916
BspFNI CGCG 2 cut(s) 275, 619
BspHI TCATGA 1 cut(s) 954
BspLI GGNNCC 3 cut(s) 332, 412, 829
BspPI GGATC 1 cut(s) 384
BspTI CTTAAG 1 cut(s) 671
BsrFI RCCGGY 1 cut(s) 89
BsrI ACTGG 1 cut(s) 778
BssAI RCCGGY 1 cut(s) 89
BssECI CCNNGG 1 cut(s) 106
BssMI GATC 2 cut(s) 376, 423
Bst2UI CCWGG 2 cut(s) 108, 744
Bst4CI ACNGT 2 cut(s) 230, 239
BstAFI CTTAAG 1 cut(s) 671
BstDEI CTNAG 5 cut(s) 81, 393, 429, 903, 963
BstF5I GGATG 1 cut(s) 643
BstFNI CGCG 2 cut(s) 275, 619
BstKTI GATC 2 cut(s) 379, 426
BstMAI GTCTC 2 cut(s) 657, 814
BstMBI GATC 2 cut(s) 376, 423
BstNI CCWGG 2 cut(s) 108, 744
BstNSI RCATGY 3 cut(s) 205, 349, 885
BstSCI CCNGG 2 cut(s) 106, 742
BstSFI CTRYAG 1 cut(s) 543
BstUI CGCG 2 cut(s) 275, 619
BstV1I GCAGC 3 cut(s) 442, 526, 663
BstX2I RGATCY 1 cut(s) 376
BstYI RGATCY 1 cut(s) 376
BsuI GTATCC 1 cut(s) 740
BsuRI GGCC 2 cut(s) 281, 526
BtgZI GCGATG 2 cut(s) 473, 609
BtsCI GGATG 1 cut(s) 643
BtsI GCAGTG 1 cut(s) 897
BtsIMutI CAGTG 2 cut(s) 244, 897
CciI TCATGA 1 cut(s) 954
Cfr10I RCCGGY 1 cut(s) 89
Csp6I GTAC 1 cut(s) 897
CspAI ACCGGT 1 cut(s) 89
CspCI CAANNNNNGTGG 2 cut(s) 133, 168
CviAII CATG 7 cut(s) 202, 346, 435, 451, 662, 882, 955
CviQI GTAC 1 cut(s) 897
DdeI CTNAG 5 cut(s) 81, 393, 429, 903, 963
DpnI GATC 2 cut(s) 378, 425
DpnII GATC 2 cut(s) 376, 423
Eco57I CTGAAG 2 cut(s) 555, 875
EcoRII CCWGG 2 cut(s) 106, 742
EcoT22I ATGCAT 2 cut(s) 663, 883
FaeI CATG 7 cut(s) 205, 349, 438, 454, 665, 885, 958
FaqI GGGAC 1 cut(s) 38
FatI CATG 7 cut(s) 201, 345, 434, 450, 661, 881, 954
FauI CCCGC 1 cut(s) 472
Fnu4HI GCNGC 3 cut(s) 456, 540, 677
FokI GGATG 1 cut(s) 650
Fsp4HI GCNGC 3 cut(s) 456, 540, 677
FspBI CTAG 1 cut(s) 336
GluI GCNGC 3 cut(s) 456, 540, 677
GsuI CTGGAG 2 cut(s) 795, 918
HaeIII GGCC 2 cut(s) 281, 526
HapII CCGG 1 cut(s) 90
Hin1II CATG 7 cut(s) 205, 349, 438, 454, 665, 885, 958
HincII GTYRAC 2 cut(s) 211, 766
HindII GTYRAC 2 cut(s) 211, 766
HindIII AAGCTT 1 cut(s) 668
HinfI GANTC 4 cut(s) 83, 359, 483, 785
HpaI GTTAAC 1 cut(s) 211
HpaII CCGG 1 cut(s) 90
HphI GGTGA 5 cut(s) 44, 133, 194, 317, 794
Hpy166II GTNNAC 5 cut(s) 87, 211, 235, 766, 897
Hpy188I TCNGA 3 cut(s) 364, 430, 906
Hpy188III TCNNGA 3 cut(s) 352, 385, 955
Hpy8I GTNNAC 5 cut(s) 87, 211, 235, 766, 897
HpyAV CCTTC 6 cut(s) 236, 537, 542, 628, 839, 850
HpyCH4III ACNGT 2 cut(s) 230, 239
HpyCH4IV ACGT 2 cut(s) 507, 608
HpyCH4V TGCA 7 cut(s) 266, 661, 708, 800, 875, 881, 923
HpyF3I CTNAG 5 cut(s) 81, 393, 429, 903, 963
HpySE526I ACGT 2 cut(s) 507, 608
Hsp92II CATG 7 cut(s) 205, 349, 438, 454, 665, 885, 958
KspAI GTTAAC 1 cut(s) 211
Kzo9I GATC 2 cut(s) 376, 423
LmnI GCTCC 5 cut(s) 121, 776, 833, 887, 911
Lsp1109I GCAGC 3 cut(s) 442, 526, 663
LweI GCATC 4 cut(s) 466, 561, 616, 931
MaeI CTAG 1 cut(s) 336
MaeII ACGT 2 cut(s) 507, 608
MaeIII GTNAC 3 cut(s) 286, 604, 685
MalI GATC 2 cut(s) 378, 425
MboI GATC 2 cut(s) 376, 423
MboII GAAGA 3 cut(s) 232, 235, 307
MflI RGATCY 1 cut(s) 376
MhlI GDGCHC 1 cut(s) 118
MluCI AATT 1 cut(s) 725
MluI ACGCGT 1 cut(s) 617
MlyI GAGTC 2 cut(s) 92, 492
MnlI CCTC 3 cut(s) 528, 565, 595
Mph1103I ATGCAT 2 cut(s) 663, 883
MseI TTAA 2 cut(s) 210, 672
MspA1I CMGCKG 1 cut(s) 938
MspCI CTTAAG 1 cut(s) 671
MspI CCGG 1 cut(s) 90
MspR9I CCNGG 2 cut(s) 108, 744
MvaI CCWGG 2 cut(s) 108, 744
MvnI CGCG 2 cut(s) 275, 619
NdeII GATC 2 cut(s) 376, 423
NlaIII CATG 7 cut(s) 205, 349, 438, 454, 665, 885, 958
NlaIV GGNNCC 3 cut(s) 332, 412, 829
NmuCI GTSAC 1 cut(s) 286
NsiI ATGCAT 2 cut(s) 663, 883
NspI RCATGY 3 cut(s) 205, 349, 885
PagI TCATGA 1 cut(s) 954
PciI ACATGT 1 cut(s) 201
PfeI GAWTC 2 cut(s) 359, 785
PflFI GACNNNGTC 1 cut(s) 50
PinAI ACCGGT 1 cut(s) 89
PkrI GCNGC 3 cut(s) 457, 541, 678
PleI GAGTC 2 cut(s) 91, 491
PpsI GAGTC 2 cut(s) 91, 491
PscI ACATGT 1 cut(s) 201
Psp1406I AACGTT 2 cut(s) 507, 608
Psp6I CCWGG 2 cut(s) 106, 742
PspGI CCWGG 2 cut(s) 106, 742
PspN4I GGNNCC 3 cut(s) 332, 412, 829
PsuI RGATCY 1 cut(s) 376
PsyI GACNNNGTC 1 cut(s) 50
PvuII CAGCTG 1 cut(s) 938
RsaI GTAC 1 cut(s) 898
RsaNI GTAC 1 cut(s) 897
SaqAI TTAA 2 cut(s) 210, 672
SatI GCNGC 3 cut(s) 456, 540, 677
Sau3AI GATC 2 cut(s) 376, 423
SchI GAGTC 2 cut(s) 92, 492
ScrFI CCNGG 2 cut(s) 108, 744
SduI GDGCHC 1 cut(s) 118
SfaNI GCATC 4 cut(s) 466, 561, 616, 931
SfcI CTRYAG 1 cut(s) 543
SmlI CTYRAG 1 cut(s) 671
SmoI CTYRAG 1 cut(s) 671
Sse9I AATT 1 cut(s) 725
SsiI CCGC 5 cut(s) 273, 341, 468, 479, 629
SspMI CTAG 1 cut(s) 336
StyD4I CCNGG 2 cut(s) 106, 742
TaaI ACNGT 2 cut(s) 230, 239
TaiI ACGT 2 cut(s) 510, 611
TaqI TCGA 2 cut(s) 45, 837
TasI AATT 1 cut(s) 725
TatI WGTACW 1 cut(s) 896
TfiI GAWTC 2 cut(s) 359, 785
Tru1I TTAA 2 cut(s) 210, 672
Tru9I TTAA 2 cut(s) 210, 672
TscAI CASTG 2 cut(s) 244, 897
TseFI GTSAC 1 cut(s) 286
TseI GCWGC 3 cut(s) 455, 539, 676
Tsp45I GTSAC 1 cut(s) 286
TspDTI ATGAA 5 cut(s) 157, 476, 777, 858, 971
TspRI CASTG 2 cut(s) 244, 897
Tth111I GACNNNGTC 1 cut(s) 50
Vha464I CTTAAG 1 cut(s) 671
XapI RAATTY 1 cut(s) 725
XceI RCATGY 3 cut(s) 205, 349, 885
XspI CTAG 1 cut(s) 336
Zsp2I ATGCAT 2 cut(s) 663, 883
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.