Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0012543

Physical Location & Seq

Genomic Coordinates Reverse (-)

32733747 .. 32735583

Gene Structure 1837 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1267100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 984 bp

ATGGCGAATTCAGTGAGTCCACTAATTGTAGCGTTCGGGGAGATGCTCATCGACTTTGTGCCAGACACGGCCGGAGTTTCGTTGGCCGAGTCCACGGGGTTCCTCAAGGCCGCCGGCGGTGCACCTGCCAATGTGGCTTGTGCCATCACCAAGCTTGGTGGTAAATCGGCTTTCGTTGGCAAGGTTGGAGATGACGAGTTCGGACACATGCTGATCAACATATTGAAGAAGAACGGGGTGAACGCAGAGGGAGTGTGCGTGGATAAGCATGCCAGAACAGCTTTGGCGTTCGTGACCTTGAAGAACGACGGTGAAAGGGAGTTCATGTTTTACCGGAACCCAAGTGCTGATATGTTGCTCAAGGATTCGGAGCTAAACATGGGTCTTATCAAGCAGGCTAAGATATTCCACTACGGATCCATAAGCCTCATAGCCGAGCCATGCAGATCGGCTCATATGGCAGCCATGAAAGCCGCTAAGGAAGCTGGCGTTTTGCTTTCCTACGATCCCAACGTCAGGTTGCCCCTCTGGCCCTCTGCTGATGCTGCTAGGCAGGAGATCAAGAGCATATGGAATCAGGCTGATTTCATCAAGGTGAGCGACGATGAGGTGAACTTCTTGACGCAAGGAGATTCTGATAAAGAAGATGTGGTTCTGTCCCTGTGGCATGACAACCTCAAACTGCTCGTGGTCACTGATGGTGAGAAGGGCTGCAGATATTACACTAAGAAATTCAAAGGAAAGGTGGCCGGCTTCTCAGTTAAAGCCGTGGACACAACCGGCGCCGGTGATTCTTTTGTTGGTTCATTTTTGCTTTCCATGGCCAATGATATGTCCATCTTTGAGGACGAAGCAAAATTGAAGGAGGCTCTGAGTTTTGCAAATGCTTGTGGAGCTATATGTACAACTCAAAAGGGTGCAATTCCAGCTCTTCCAACCCAGTCTGATGCCCATGAGCTCATTTCCAAGTCCAAAGCCAAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004396 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0005996 GO:0006000 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0008865 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019200 GO:0019318 GO:0044237 GO:0044238 GO:0044262 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046835 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00051 ko00500 ko00520 ko01100 map00051 map00500 map00520 map01100

Pfam Domains

Protein Families

PfkB

Protein Analysis

328

Amino Acids

35.01

Weight (kDa)

5.92

Isoelectric Point (pI)

22.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PfkB	PF00294	8 - 313	2.2e-76	pfkB family carbohydrate kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012543)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G10260
fragaria_vesca	FvH4_2g00080
malus_domestica	MD17G1267100.v1.1
prunus_persica	Prupe.8G000700_v2.0.a1
pyrus_communis	pycom17g26620
rosa_chinensis	RchiOBHm_Chr6g0249341
rosa_laevigata	RLG00000015590
rosa_multiflora	Rmu_co8334337.1_g000001 Rmu_sc0000278.1_g000022 Rmu_sc0006234.1_g000016
rosa_roxburghii	Rroxscaffold_7G00217830
rosa_rugosa	Rorug05G0490600.1 Rorug05G0490700.1 Rorug05G0490800.1
rosa_samantha	Rh6AG001300 Rh6BG009000 Rh6CG000400 Rh6DG000300
rosa_wichuraiana	Rw6G000190

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	133
Acc36I	ACCTGC	1 cut(s)	133
AccB1I	GGYRCC	1 cut(s)	782
AciI	CCGC	3 cut(s)	111, 117, 474
AclWI	GGATC	3 cut(s)	411, 424, 500
AcoI	YGGCCR	4 cut(s)	69, 84, 747, 822
AcsI	RAATTY	2 cut(s)	7, 731
AcyI	GRCGYC	1 cut(s)	783
AfaI	GTAC	1 cut(s)	904
AfiI	CCNNNNNNNGG	1 cut(s)	516
AgsI	TTSAA	4 cut(s)	226, 301, 736, 862
AluBI	AGCT	7 cut(s)	154, 281, 373, 485, 896, 929, 958
AluI	AGCT	7 cut(s)	154, 281, 373, 485, 896, 929, 958
Alw21I	GWGCWC	2 cut(s)	124, 960
Alw44I	GTGCAC	1 cut(s)	120
AlwI	GGATC	3 cut(s)	411, 424, 500
AoxI	GGCC	6 cut(s)	69, 84, 108, 530, 747, 822
ApaLI	GTGCAC	1 cut(s)	120
ApeKI	GCWGC	3 cut(s)	461, 545, 711
ApoI	RAATTY	2 cut(s)	7, 731
AspLEI	GCGC	1 cut(s)	785
AspS9I	GGNCC	1 cut(s)	531
AsuHPI	GGTGA	7 cut(s)	139, 250, 323, 607, 622, 713, 800
BaeGI	GKGCMC	1 cut(s)	124
BalI	TGGCCA	1 cut(s)	824
BamHI	GGATCC	1 cut(s)	416
BanI	GGYRCC	1 cut(s)	782
BanII	GRGCYC	1 cut(s)	960
BauI	CACGAG	1 cut(s)	686
Bbv12I	GWGCWC	2 cut(s)	124, 960
BbvI	GCAGC	3 cut(s)	473, 532, 698
BccI	CCATC	3 cut(s)	152, 692, 845
BceAI	ACGGC	2 cut(s)	84, 752
BclI	TGATCA	1 cut(s)	213
BfaI	CTAG	1 cut(s)	549
BfmI	CTRYAG	1 cut(s)	712
BfoI	RGCGCY	1 cut(s)	786
BfuAI	ACCTGC	1 cut(s)	133
BglI	GCCNNNNNGGC	2 cut(s)	134, 529
BisI	GCNGC	5 cut(s)	111, 462, 474, 546, 712
BlsI	GCNGC	5 cut(s)	112, 463, 475, 547, 713
BmgT120I	GGNCC	1 cut(s)	531
BmiI	GGNNCC	4 cut(s)	101, 338, 418, 784
BmrI	ACTGGG	1 cut(s)	934
BmsI	GCATC	3 cut(s)	33, 532, 937
BmuI	ACTGGG	1 cut(s)	934
Bpu10I	CCTNAGC	1 cut(s)	477
BpuEI	CTTGAG	2 cut(s)	89, 344
BsaBI	GATNNNNATC	1 cut(s)	47
BsaHI	GRCGYC	1 cut(s)	783
BsaJI	CCNNGG	3 cut(s)	93, 768, 819
BsaWI	WCCGGW	1 cut(s)	333
BsaXI	ACNNNNNCTCC	4 cut(s)	243, 273, 311, 341
Bsc4I	CCNNNNNNNGG	1 cut(s)	516
Bse118I	RCCGGY	4 cut(s)	113, 749, 779, 785
Bse1I	ACTGG	1 cut(s)	940
Bse8I	GATNNNNATC	1 cut(s)	47
BseDI	CCNNGG	3 cut(s)	93, 768, 819
BseJI	GATNNNNATC	1 cut(s)	47
BseLI	CCNNNNNNNGG	1 cut(s)	516
BseMII	CTCAG	2 cut(s)	771, 863
BseNI	ACTGG	1 cut(s)	940
BseSI	GKGCMC	1 cut(s)	124
BseX3I	CGGCCG	1 cut(s)	69
BseXI	GCAGC	3 cut(s)	473, 532, 698
Bsh1285I	CGRYCG	1 cut(s)	72
BshFI	GGCC	6 cut(s)	71, 86, 110, 532, 749, 824
BshNI	GGYRCC	1 cut(s)	782
BsiEI	CGRYCG	1 cut(s)	72
BsiHKAI	GWGCWC	2 cut(s)	124, 960
BsiSI	CCGG	6 cut(s)	72, 114, 334, 750, 780, 786
BslFI	GGGAC	1 cut(s)	643
BslI	CCNNNNNNNGG	1 cut(s)	516
BsmFI	GGGAC	1 cut(s)	643
BsnI	GGCC	6 cut(s)	71, 86, 110, 532, 749, 824
Bsp1286I	GDGCHC	2 cut(s)	124, 960
Bsp1407I	TGTACA	1 cut(s)	902
Bsp143I	GATC	5 cut(s)	213, 416, 446, 505, 558
Bsp19I	CCATGG	1 cut(s)	819
BspACI	CCGC	3 cut(s)	111, 117, 474
BspANI	GGCC	6 cut(s)	71, 86, 110, 532, 749, 824
BspCNI	CTCAG	2 cut(s)	770, 864
BspLI	GGNNCC	4 cut(s)	101, 338, 418, 784
BspMAI	CTGCAG	1 cut(s)	716
BspMI	ACCTGC	1 cut(s)	133
BspPI	GGATC	3 cut(s)	411, 424, 500
BspQI	GCTCTTC	1 cut(s)	936
BspT107I	GGYRCC	1 cut(s)	782
BsrFI	RCCGGY	4 cut(s)	113, 749, 779, 785
BsrGI	TGTACA	1 cut(s)	902
BsrI	ACTGG	1 cut(s)	940
BssAI	RCCGGY	4 cut(s)	113, 749, 779, 785
BssECI	CCNNGG	3 cut(s)	93, 768, 819
BssMI	GATC	5 cut(s)	213, 416, 446, 505, 558
BssNI	GRCGYC	1 cut(s)	783
BssSI	CACGAG	1 cut(s)	686
BssT1I	CCWWGG	1 cut(s)	819
Bst2BI	CACGAG	1 cut(s)	686
Bst4CI	ACNGT	1 cut(s)	311
Bst6I	CTCTTC	1 cut(s)	936
BstACI	GRCGYC	1 cut(s)	783
BstAUI	TGTACA	1 cut(s)	902
BstC8I	GCNNGC	5 cut(s)	115, 270, 396, 487, 751
BstDEI	CTNAG	5 cut(s)	399, 477, 726, 757, 872
BstDSI	CCRYGG	3 cut(s)	93, 768, 819
BstH2I	RGCGCY	1 cut(s)	786
BstHHI	GCGC	1 cut(s)	785
BstKTI	GATC	5 cut(s)	216, 419, 449, 508, 561
BstMBI	GATC	5 cut(s)	213, 416, 446, 505, 558
BstMCI	CGRYCG	1 cut(s)	72
BstNSI	RCATGY	2 cut(s)	211, 272
BstSFI	CTRYAG	1 cut(s)	712
BstSLI	GKGCMC	1 cut(s)	124
BstV1I	GCAGC	3 cut(s)	473, 532, 698
BstX2I	RGATCY	1 cut(s)	416
BstYI	RGATCY	1 cut(s)	416
BstZI	CGGCCG	1 cut(s)	69
BsuRI	GGCC	6 cut(s)	71, 86, 110, 532, 749, 824
BtgI	CCRYGG	3 cut(s)	93, 768, 819
BtsIMutI	CAGTG	2 cut(s)	18, 693
BveI	ACCTGC	1 cut(s)	133
Cac8I	GCNNGC	5 cut(s)	115, 270, 396, 487, 751
CfoI	GCGC	1 cut(s)	785
Cfr10I	RCCGGY	4 cut(s)	113, 749, 779, 785
Cfr13I	GGNCC	1 cut(s)	531
CseI	GACGC	1 cut(s)	631
Csp6I	GTAC	1 cut(s)	903
CspCI	CAANNNNNGTGG	2 cut(s)	139, 174
CviAII	CATG	9 cut(s)	208, 269, 325, 379, 441, 466, 668, 820, 953
CviQI	GTAC	1 cut(s)	903
DdeI	CTNAG	5 cut(s)	399, 477, 726, 757, 872
DinI	GGCGCC	1 cut(s)	784
DpnI	GATC	5 cut(s)	215, 418, 448, 507, 560
DpnII	GATC	5 cut(s)	213, 416, 446, 505, 558
EaeI	YGGCCR	4 cut(s)	69, 84, 747, 822
EagI	CGGCCG	1 cut(s)	69
Eam1104I	CTCTTC	1 cut(s)	936
EarI	CTCTTC	1 cut(s)	936
Ecl136II	GAGCTC	1 cut(s)	958
EclXI	CGGCCG	1 cut(s)	69
Eco130I	CCWWGG	1 cut(s)	819
Eco24I	GRGCYC	1 cut(s)	960
Eco52I	CGGCCG	1 cut(s)	69
Eco53kI	GAGCTC	1 cut(s)	958
EcoICRI	GAGCTC	1 cut(s)	958
EcoRI	GAATTC	1 cut(s)	7
EcoT14I	CCWWGG	1 cut(s)	819
EcoT38I	GRGCYC	1 cut(s)	960
EgeI	GGCGCC	1 cut(s)	784
EheI	GGCGCC	1 cut(s)	784
ErhI	CCWWGG	1 cut(s)	819
FaeI	CATG	9 cut(s)	211, 272, 328, 382, 444, 469, 671, 823, 956
FaqI	GGGAC	1 cut(s)	643
FatI	CATG	9 cut(s)	207, 268, 324, 378, 440, 465, 667, 819, 952
FauNDI	CATATG	2 cut(s)	456, 569
FbaI	TGATCA	1 cut(s)	213
Fnu4HI	GCNGC	5 cut(s)	111, 462, 474, 546, 712
FriOI	GRGCYC	1 cut(s)	960
Fsp4HI	GCNGC	5 cut(s)	111, 462, 474, 546, 712
FspBI	CTAG	1 cut(s)	549
GlaI	GCGC	1 cut(s)	784
GluI	GCNGC	5 cut(s)	111, 462, 474, 546, 712
HaeII	RGCGCY	1 cut(s)	786
HaeIII	GGCC	6 cut(s)	71, 86, 110, 532, 749, 824
HapII	CCGG	6 cut(s)	72, 114, 334, 750, 780, 786
HgaI	GACGC	1 cut(s)	631
HhaI	GCGC	1 cut(s)	785
Hin1I	GRCGYC	1 cut(s)	783
Hin1II	CATG	9 cut(s)	211, 272, 328, 382, 444, 469, 671, 823, 956
Hin6I	GCGC	1 cut(s)	783
HinP1I	GCGC	1 cut(s)	783
HindIII	AAGCTT	1 cut(s)	152
HinfI	GANTC	6 cut(s)	16, 89, 365, 574, 632, 791
HpaII	CCGG	6 cut(s)	72, 114, 334, 750, 780, 786
HphI	GGTGA	7 cut(s)	139, 250, 323, 607, 622, 713, 800
Hpy166II	GTNNAC	6 cut(s)	20, 93, 122, 241, 613, 772
Hpy188I	TCNGA	5 cut(s)	203, 370, 637, 873, 946
Hpy188III	TCNNGA	3 cut(s)	292, 562, 619
Hpy8I	GTNNAC	6 cut(s)	20, 93, 122, 241, 613, 772
Hpy99I	CGWCG	2 cut(s)	311, 605
HpyAV	CCTTC	2 cut(s)	700, 856
HpyCH4III	ACNGT	1 cut(s)	311
HpyCH4IV	ACGT	1 cut(s)	513
HpyCH4V	TGCA	5 cut(s)	122, 444, 714, 881, 920
HpyF3I	CTNAG	5 cut(s)	399, 477, 726, 757, 872
HpySE526I	ACGT	1 cut(s)	513
Hsp92I	GRCGYC	1 cut(s)	783
Hsp92II	CATG	9 cut(s)	211, 272, 328, 382, 444, 469, 671, 823, 956
HspAI	GCGC	1 cut(s)	783
KasI	GGCGCC	1 cut(s)	782
KroI	GCCGGC	2 cut(s)	113, 749
KroNI	GCCGGC	2 cut(s)	115, 751
Ksp22I	TGATCA	1 cut(s)	213
Kzo9I	GATC	5 cut(s)	213, 416, 446, 505, 558
LguI	GCTCTTC	1 cut(s)	936
LmnI	GCTCC	2 cut(s)	370, 893
Lsp1109I	GCAGC	3 cut(s)	473, 532, 698
LweI	GCATC	3 cut(s)	33, 532, 937
MaeI	CTAG	1 cut(s)	549
MaeII	ACGT	1 cut(s)	513
MaeIII	GTNAC	2 cut(s)	292, 691
MalI	GATC	5 cut(s)	215, 418, 448, 507, 560
MboI	GATC	5 cut(s)	213, 416, 446, 505, 558
MboII	GAAGA	5 cut(s)	238, 241, 313, 656, 923
MflI	RGATCY	1 cut(s)	416
MhlI	GDGCHC	2 cut(s)	124, 960
MlsI	TGGCCA	1 cut(s)	824
MluCI	AATT	5 cut(s)	7, 24, 731, 857, 921
MluNI	TGGCCA	1 cut(s)	824
Mly113I	GGCGCC	1 cut(s)	783
MlyI	GAGTC	2 cut(s)	25, 98
MmeI	TCCRAC	2 cut(s)	166, 959
MnlI	CCTC	9 cut(s)	113, 241, 437, 536, 544, 601, 686, 838, 859
Mox20I	TGGCCA	1 cut(s)	824
MreI	CGCCGGCG	1 cut(s)	113
MroNI	GCCGGC	2 cut(s)	113, 749
MscI	TGGCCA	1 cut(s)	824
MseI	TTAA	1 cut(s)	762
MslI	CAYNNNNRTG	1 cut(s)	593
Msp20I	TGGCCA	1 cut(s)	824
MspI	CCGG	6 cut(s)	72, 114, 334, 750, 780, 786
NaeI	GCCGGC	2 cut(s)	115, 751
NarI	GGCGCC	1 cut(s)	783
NcoI	CCATGG	1 cut(s)	819
NdeI	CATATG	2 cut(s)	456, 569
NdeII	GATC	5 cut(s)	213, 416, 446, 505, 558
NgoMIV	GCCGGC	2 cut(s)	113, 749
NlaIII	CATG	9 cut(s)	211, 272, 328, 382, 444, 469, 671, 823, 956
NlaIV	GGNNCC	4 cut(s)	101, 338, 418, 784
NmeAIII	GCCGAG	2 cut(s)	112, 460
NmuCI	GTSAC	2 cut(s)	292, 691
NspI	RCATGY	2 cut(s)	211, 272
PaeI	GCATGC	1 cut(s)	272
PaqCI	CACCTGC	1 cut(s)	133
PciSI	GCTCTTC	1 cut(s)	936
PcsI	WCGNNNNNNNCGW	1 cut(s)	510
PdiI	GCCGGC	2 cut(s)	115, 751
PfeI	GAWTC	4 cut(s)	365, 574, 632, 791
PkrI	GCNGC	5 cut(s)	112, 463, 475, 547, 713
PleI	GAGTC	2 cut(s)	24, 97
PluTI	GGCGCC	1 cut(s)	786
PpsI	GAGTC	2 cut(s)	24, 97
Psp124BI	GAGCTC	1 cut(s)	960
PspN4I	GGNNCC	4 cut(s)	101, 338, 418, 784
PspPI	GGNCC	1 cut(s)	531
PstI	CTGCAG	1 cut(s)	716
PsuI	RGATCY	1 cut(s)	416
RsaI	GTAC	1 cut(s)	904
RsaNI	GTAC	1 cut(s)	903
RseI	CAYNNNNRTG	1 cut(s)	593
SacI	GAGCTC	1 cut(s)	960
SapI	GCTCTTC	1 cut(s)	936
SaqAI	TTAA	1 cut(s)	762
SatI	GCNGC	5 cut(s)	111, 462, 474, 546, 712
Sau3AI	GATC	5 cut(s)	213, 416, 446, 505, 558
Sau96I	GGNCC	1 cut(s)	531
SchI	GAGTC	2 cut(s)	25, 98
SduI	GDGCHC	2 cut(s)	124, 960
SfaNI	GCATC	3 cut(s)	33, 532, 937
SfcI	CTRYAG	1 cut(s)	712
SfoI	GGCGCC	1 cut(s)	784
SgrAI	CRCCGGYG	2 cut(s)	113, 785
SmiMI	CAYNNNNRTG	1 cut(s)	593
SmlI	CTYRAG	2 cut(s)	104, 359
SmoI	CTYRAG	2 cut(s)	104, 359
SphI	GCATGC	1 cut(s)	272
Sse9I	AATT	5 cut(s)	7, 24, 731, 857, 921
SsiI	CCGC	3 cut(s)	111, 117, 474
SspDI	GGCGCC	1 cut(s)	782
SspMI	CTAG	1 cut(s)	549
SstI	GAGCTC	1 cut(s)	960
StyI	CCWWGG	1 cut(s)	819
TaaI	ACNGT	1 cut(s)	311
TaiI	ACGT	1 cut(s)	516
TaqI	TCGA	1 cut(s)	51
TasI	AATT	5 cut(s)	7, 24, 731, 857, 921
TatI	WGTACW	1 cut(s)	902
TauI	GCSGC	2 cut(s)	113, 476
TfiI	GAWTC	4 cut(s)	365, 574, 632, 791
Tru1I	TTAA	1 cut(s)	762
Tru9I	TTAA	1 cut(s)	762
TscAI	CASTG	2 cut(s)	18, 700
TseFI	GTSAC	2 cut(s)	292, 691
TseI	GCWGC	3 cut(s)	461, 545, 711
Tsp45I	GTSAC	2 cut(s)	292, 691
TspDTI	ATGAA	4 cut(s)	313, 482, 577, 795
TspGWI	ACGGA	1 cut(s)	429
TspRI	CASTG	2 cut(s)	18, 700
VneI	GTGCAC	1 cut(s)	120
XapI	RAATTY	2 cut(s)	7, 731
XceI	RCATGY	2 cut(s)	211, 272
XcmI	CCANNNNNNNNNTGG	1 cut(s)	280
XspI	CTAG	1 cut(s)	549

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.