AT4G15520

tRNA rRNA methyltransferase (SpoU) family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0015140
Physical Location & Seq
Forward (+)
8862744 .. 8864646
1903 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G15520.1

Sequence Viewer

Length: 669 bp
ATGGCGGGATTTGAGAGCTATGTGGTTGTTCACAACATAGCAAAGCGTCACAACGTCGGAACTTTAGCTCGTAGCGCCACTGCCTTTGGTGTGACGGAGCTAATCCTCGTCGGTCGCCGTGATTTCAACGCCTTCGGTAGTCATGGTTCAGCTTCTCACATCCGTTTCCGCCATTTTCACTCTCTCATCGAAGCTCGCAACTATCTCAAGGAGGAGAAAGATTGCGATATATGCGGCGTTGAGATTGCAGATGGAGCTTCTGCGGTTAATGAACATCCTTTCAAAAGAAACACTGCGTTTCTTCTCGGTAATGAGGGATCAGGATTATCTGCGAAGGAATACGAAATATGCGACTTCTTTGTATATATTCCTCAGTATGGCTGTGGCACTGCCTCTTTGAATGTTACTGTTGCAGCTTCTATTGTTTTGCATCACTTTGGAGTTTGGGCTGGATTTTCTGAACGTGTTCGGGATGGAAGCAAATTTATAGTGGCTGATAGACCCGTAAGACAAGGAAGACGAAATTTCTGCGCCGGCACAGAAGAATCCGTCATTGAAGAGCGGAAGTTGAGGAAAGAAAGTGCAGAAAATGGGTTTTTCGATGACAATGGAAATGAAAATGGAAGTTCATCATCCGACCTTTTGGACGGTTTGTTCCTTAACGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SpoU_methylase

Protein Analysis

222

Amino Acids

24.46

Weight (kDa)

5.86

Isoelectric Point (pI)

41.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SpoU_methylase PF00588 7 - 144 1.4e-30 SpoU rRNA Methylase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015140)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15520
fragaria_vesca FvH4_4g16710
malus_domestica MD13G1177300.v1.1
prunus_persica Prupe.1G146500_v2.0.a1 Prupe.1G146500_v2.0.a1
pyrus_communis pycom13g15280 pycom16g15030
rosa_chinensis RchiOBHm_Chr4g0420161
rosa_laevigata RLG00000007785
rosa_multiflora Rmu_sc0004455.1_g000001
rosa_roxburghii Rroxscaffold_5G00362860
rosa_rugosa Rorug04G0162700
rosa_samantha Rh4AG223500 Rh4BG226100 Rh4DG222300
rosa_wichuraiana Rw4G019240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 562
AciI CCGC 5 cut(s) 5, 169, 234, 263, 562
AclWI GGATC 1 cut(s) 325
AcsI RAATTY 2 cut(s) 482, 523
AfiI CCNNNNNNNGG 1 cut(s) 377
AflIII ACRYGT 1 cut(s) 463
AgsI TTSAA 4 cut(s) 127, 283, 400, 557
AloI GAACNNNNNNTCC 1 cut(s) 638
AluBI AGCT 7 cut(s) 18, 68, 100, 152, 194, 257, 416
AluI AGCT 7 cut(s) 18, 68, 100, 152, 194, 257, 416
AlwI GGATC 1 cut(s) 325
ApeKI GCWGC 1 cut(s) 413
ApoI RAATTY 2 cut(s) 482, 523
Asp700I GAANNNNTTC 1 cut(s) 465
AspLEI GCGC 2 cut(s) 77, 533
BbsI GAAGAC 1 cut(s) 523
BbvI GCAGC 1 cut(s) 425
BccI CCATC 2 cut(s) 245, 467
BceAI ACGGC 1 cut(s) 102
BcgI CGANNNNNNTGC 2 cut(s) 510, 544
BfoI RGCGCY 1 cut(s) 78
BisI GCNGC 2 cut(s) 235, 414
BlsI GCNGC 2 cut(s) 236, 415
BmsI GCATC 1 cut(s) 439
BpiI GAAGAC 1 cut(s) 523
BpuEI CTTGAG 1 cut(s) 191
Bsc4I CCNNNNNNNGG 1 cut(s) 377
Bse118I RCCGGY 1 cut(s) 533
BseGI GGATG 4 cut(s) 159, 274, 478, 632
BseLI CCNNNNNNNGG 1 cut(s) 377
BseMII CTCAG 1 cut(s) 386
BseRI GAGGAG 1 cut(s) 227
BseXI GCAGC 1 cut(s) 425
BsgI GTGCAG 1 cut(s) 603
Bsh1285I CGRYCG 1 cut(s) 115
BsiEI CGRYCG 1 cut(s) 115
BsiSI CCGG 1 cut(s) 534
BslI CCNNNNNNNGG 1 cut(s) 377
Bsp143I GATC 1 cut(s) 317
BspACI CCGC 5 cut(s) 5, 169, 234, 263, 562
BspCNI CTCAG 1 cut(s) 385
BspPI GGATC 1 cut(s) 325
BspQI GCTCTTC 1 cut(s) 552
BsrBI CCGCTC 1 cut(s) 562
BsrFI RCCGGY 1 cut(s) 533
BssAI RCCGGY 1 cut(s) 533
BssMI GATC 1 cut(s) 317
Bst4CI ACNGT 2 cut(s) 409, 650
Bst6I CTCTTC 1 cut(s) 552
BstC8I GCNNGC 2 cut(s) 196, 535
BstDEI CTNAG 1 cut(s) 372
BstF5I GGATG 4 cut(s) 159, 274, 478, 632
BstH2I RGCGCY 1 cut(s) 78
BstHHI GCGC 2 cut(s) 77, 533
BstKTI GATC 1 cut(s) 320
BstMBI GATC 1 cut(s) 317
BstMCI CGRYCG 1 cut(s) 115
BstMWI GCNNNNNNNGC 3 cut(s) 74, 231, 254
BstV1I GCAGC 1 cut(s) 425
BstV2I GAAGAC 1 cut(s) 523
BtsCI GGATG 4 cut(s) 159, 274, 478, 632
BtsI GCAGTG 3 cut(s) 78, 291, 387
BtsIMutI CAGTG 3 cut(s) 78, 291, 387
Cac8I GCNNGC 2 cut(s) 196, 535
CfoI GCGC 2 cut(s) 77, 533
Cfr10I RCCGGY 1 cut(s) 533
CseI GACGC 1 cut(s) 35
CspCI CAANNNNNGTGG 2 cut(s) 67, 102
CviAII CATG 1 cut(s) 143
DdeI CTNAG 1 cut(s) 372
DpnI GATC 1 cut(s) 319
DpnII GATC 1 cut(s) 317
Eam1104I CTCTTC 1 cut(s) 552
EarI CTCTTC 1 cut(s) 552
EciI GGCGGA 1 cut(s) 158
FaeI CATG 1 cut(s) 146
FatI CATG 1 cut(s) 142
Fnu4HI GCNGC 2 cut(s) 235, 414
FokI GGATG 4 cut(s) 146, 261, 485, 619
Fsp4HI GCNGC 2 cut(s) 235, 414
GlaI GCGC 2 cut(s) 76, 532
GluI GCNGC 2 cut(s) 235, 414
HaeII RGCGCY 1 cut(s) 78
HapII CCGG 1 cut(s) 534
HgaI GACGC 1 cut(s) 35
HhaI GCGC 2 cut(s) 77, 533
Hin1II CATG 1 cut(s) 146
Hin6I GCGC 2 cut(s) 75, 531
HinP1I GCGC 2 cut(s) 75, 531
HinfI GANTC 1 cut(s) 545
HpaII CCGG 1 cut(s) 534
Hpy166II GTNNAC 1 cut(s) 31
Hpy188I TCNGA 3 cut(s) 59, 460, 637
Hpy188III TCNNGA 2 cut(s) 321, 470
Hpy8I GTNNAC 1 cut(s) 31
Hpy99I CGWCG 2 cut(s) 59, 113
HpyAV CCTTC 2 cut(s) 142, 328
HpyCH4III ACNGT 2 cut(s) 409, 650
HpyCH4IV ACGT 2 cut(s) 54, 463
HpyCH4V TGCA 4 cut(s) 248, 413, 430, 584
HpyF10VI GCNNNNNNNGC 3 cut(s) 74, 231, 254
HpyF3I CTNAG 1 cut(s) 372
HpySE526I ACGT 2 cut(s) 54, 463
Hsp92II CATG 1 cut(s) 146
HspAI GCGC 2 cut(s) 75, 531
KroI GCCGGC 1 cut(s) 533
KroNI GCCGGC 1 cut(s) 535
Kzo9I GATC 1 cut(s) 317
LguI GCTCTTC 1 cut(s) 552
LmnI GCTCC 2 cut(s) 97, 254
LpnPI CCDG 3 cut(s) 306, 435, 547
Lsp1109I GCAGC 1 cut(s) 425
LweI GCATC 1 cut(s) 439
MaeII ACGT 2 cut(s) 54, 463
MaeIII GTNAC 3 cut(s) 47, 91, 403
MalI GATC 1 cut(s) 319
MbiI CCGCTC 1 cut(s) 562
MboI GATC 1 cut(s) 317
MboII GAAGA 4 cut(s) 293, 528, 554, 569
MluCI AATT 2 cut(s) 482, 523
MmeI TCCRAC 2 cut(s) 37, 660
MnlI CCTC 6 cut(s) 116, 205, 307, 381, 403, 564
MroNI GCCGGC 1 cut(s) 533
MroXI GAANNNNTTC 1 cut(s) 465
MseI TTAA 2 cut(s) 267, 660
MspI CCGG 1 cut(s) 534
MwoI GCNNNNNNNGC 3 cut(s) 74, 231, 254
NaeI GCCGGC 1 cut(s) 535
NdeII GATC 1 cut(s) 317
NgoMIV GCCGGC 1 cut(s) 533
NlaIII CATG 1 cut(s) 146
NmuCI GTSAC 2 cut(s) 47, 91
PciSI GCTCTTC 1 cut(s) 552
PcsI WCGNNNNNNNCGW 1 cut(s) 348
PdiI GCCGGC 1 cut(s) 535
PdmI GAANNNNTTC 1 cut(s) 465
PfeI GAWTC 1 cut(s) 545
PkrI GCNGC 2 cut(s) 236, 415
PsrI GAACNNNNNNTAC 2 cut(s) 130, 162
SapI GCTCTTC 1 cut(s) 552
SaqAI TTAA 2 cut(s) 267, 660
SatI GCNGC 2 cut(s) 235, 414
Sau3AI GATC 1 cut(s) 317
SfaNI GCATC 1 cut(s) 439
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
Sse9I AATT 2 cut(s) 482, 523
SsiI CCGC 5 cut(s) 5, 169, 234, 263, 562
TaaI ACNGT 2 cut(s) 409, 650
TaiI ACGT 2 cut(s) 57, 466
TaqI TCGA 2 cut(s) 189, 600
TaqII GACCGA 1 cut(s) 101
TasI AATT 2 cut(s) 482, 523
TauI GCSGC 1 cut(s) 237
TfiI GAWTC 1 cut(s) 545
Tru1I TTAA 2 cut(s) 267, 660
Tru9I TTAA 2 cut(s) 267, 660
TscAI CASTG 3 cut(s) 85, 298, 394
TseFI GTSAC 2 cut(s) 47, 91
TseI GCWGC 1 cut(s) 413
Tsp45I GTSAC 2 cut(s) 47, 91
TspDTI ATGAA 3 cut(s) 285, 618, 630
TspGWI ACGGA 3 cut(s) 110, 152, 538
TspRI CASTG 3 cut(s) 85, 298, 394
XapI RAATTY 2 cut(s) 482, 523
XmnI GAANNNNTTC 1 cut(s) 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.