Rorug04G0162700

Protein of unknown function (DUF679)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0003054
Physical Location & Seq
Reverse (-)
28324539 .. 28334794
10256 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0162700.1

Sequence Viewer

Length: 5550 bp
ATGGAAATCTGCTTTTCAGTTCTTGGAAAACTCGCAGAGTACACAGTAGCACCCGCTCTGCGACAGTTTAGCTATCTGATTCACTGTGAGAGCAACATTAAGAATCTCACCAGCCAGGCTGGGAGGCTGGCCGACAAAAGAGATGGGATACAGCTAGCTGTGGTTGTGGCGACCAGAAACTTAGAGACTATTGCTCCTGAAGTTGAGTCCTGGCTGCATGATGTTAACAATACCATACAGGACAAGGAGACGTGTTTTGAGGAAGAAACTGTAGCAAAGGCAACATGCTCTAATGGATGGTTTCCAAATTTGAAGTATCGTCATTCCTTGAGTAGGAAAGCTAAGAAGATGACTGTGATTGTTGATAAGCTCCTTGGAGACGGGAGTTTCGCAGTCATATCTTACCCACCTCCTCCCCCAGAGATATTTCCATTTCCAAACAAAAAACATTCCGAGTATGTTGATAAGGCTTTGGCCAAGCCCCATGAAGGGGCAAGCTCCTCAGGAACCTCCAACCCTGCTCCTCCATCACTTATGAAACTATCTGAGAATTTGGAATACAGATTACCTTATACGAAAGAGGTCATGAATGCTTTGCATAATGATGATATCAACATGATTGGCATTTGCGGGATAATCAAAGGAGGAGATACGGTGACAGCAAAAGAATTCATCCAGATAGTAAAACACCAAGATATATTTGAGGAGGTTGTGATGGCAGTTGTGTCCCATGATCCAGACTTGAAGCGGATACAAGTCGAAATTGGAGATATGCTAGGGCTGAATTTGGAAACTATGAATTTGATTGAAGGAGCAGAGATGCTTCATGCTAGAATGTCTAGCGATACAGAGAGGTTCCTTGTTGTATTGGCTGATGTTTGGAAAATACTTGATTTGGAAGCAGTTGGAATCCCTCATGGTGAAAGTAACCAAGGATGCAAGATCATATTTTTGTCAAGACTGCCGAATGTATTTAGTGAGTTGAGAACTCAGAGTAATTTCAAACTGTTATTGGCATCTATATCCTTTGAAGGACCCATGGATTTTGAATCAAGAAATTCAGTCCTTACAGAAGTAATGAATGCTCTCACCGATGACCAAATAAACCCAATTGTAATATGCGGAATGCCTGGTATAGGCAAGACAACCTTGGTGAAAGAAGTTGGTAAAAAGGCAAAAGAAGGTGGCCTATTCGATGAGGTTGCAATGGCTGTTATGACCCAGAATCCTGACTTGAGTCATATTCAACATGAAATTGCAGATTTTCTGGGTTTGAAACTTATTGAACAGAGTTTATCTGGAAGAGCATATAAGTTACTTGAAAGATTATCGGGTGGTAAGAGGGTTCTTGTAATATTAGACAATGTTTGGACACCAGTAGATTTGGAAGCATTAGGTATTCCACTTTGTTGCAAAATTCTGGTATCATCACGAAGCCAAGATATAATATTCAAGAAGATGACAACCAAAAAGAACTTTTCTCTTGGTGTTTTGGAAGAATCAGAAGCATGGTGTCTGTTTAAGGAGACGGCAGGCGATTCCATTGAATCTCCAGAGCTACATTCTGTAGCACATCAGGTTCTGAGCGAGTGTGCTGGTTTGCCAATTGCAATTTCTACTGTTGGAAGGGCACTGCAACATAAAAGCAAGCCAATGTGGAATGATGCGGTTAGACAACTAAGAAAGGCTTGTCCGAAGAACATTCCAGGAGTGATACAAGAAGTGTATGGGAAAATAAAGTTGAGTTACAAATGTTTAAGTAGCAAGGAAGCCAAATCATGTTTTTTACTGTGTTGCCTATATCCAGAAAGTGGTAATATACTGGTTGAAGATTTGGTTAGGTATGGAGTTGGGCTTGGGTTCTTTAACAGCATTGATTCAATGGAAGAAGGAAGAAACAGTGTAGAAACATTGATTGACATACTCAAGAGGTGTTTTTTGTTGTTAGACAGCAACAAGAAAGGGTGCGTTAGAATGCATGATGTGGTCCGCGATGTTGCCCTATTCATATTCTCTAAGGGTCGTAAAAAGAGTTTTTTGTTAAGATGTGAAGTGGAACTGAAGGGTTGGCGTAAGATTAATGGTGCTTCCAAGCAGTACATGTGCAGTACACTGATAAACAATCAAACTCATCAGCATACTGTGCTTTTATTATTGTCAAGCATGGATAAATTGTCTTTGGAACCACCATCCACTATATTTGAAGGAATGGGAGATCTTAAGGTTTTAGGCATGAGGAGCACAGTTGTTTCGCCAATACTGCCATCGCTTCCACTTTTAAGAAACCTTCAAACGTTGTCTCTGGAGCATTGCAATTTTAACAACGATCATGTCAAGATACTCAACAGTGTCCTCAGAAAGCTACGGACACTGATGATACTCAGCTTTCGTGGCTCTTGCATCAAAGAATTACCTGATGAACTTAAAAATTTGTCGAAGCTAAGGTTGCTAGATTTGACAGGATGTAGTGGTCTTGAGATGATTTCACCAGGTGTCATTTCTAGCTTAACCCAACTAGAGGAATTATACATGTGGAATAGCTTTGACAAATGGGTGGTCCTAAGAGATGAACATGTCTCAGAAGCCCAACTAGTGTCCATGCAACTGATGCAAGAGAATCCACATCAAATGCCAGAGGAGCCGGTGAACATCTTTGAGTCAACTAGTTTTGAGGAACATAGAAAAGTAAACACTAATGCAAAACTTTCAGAGCTACTTTCCTTGAGCTGTTTGACTAGTTTAGAAGCTGCTTTTCCACCAACTAAAACTATGCAAAGCAGTGTGGCATTTCATAAGCTTGAAAGATTTAAAATCTCTATAGAATCCATGGCCGATAAGGAAAGAAGATTGGATGTAAGCCCTTCTGAGAACTACTTGAGACTTCATGATCTTGATGGAATAATGGATGAGGAAAGAAGATTGGATGTAAGCCCTTCTGAGAACTACTTGAGAATTCATGATCTTGATGGAAACTCTCTTGTGGGAAGTGCAATCTCTGTGTTACTGAAGAATACCAACACACTAGATCTGAAAATGAAAAATCTCGAGGATGCTTTCAATTTCTTAGATAGAGACTGTTTTGCAAACTTGAAGTCTCTAACACTGCAAGATTGTAAGGCTATGGAATATCTCATTGACATGACGGATATAGTTCCACTTCCACGTAATGGAGTGCTTCCGGTCTTAGATTCGCTGAATATCCGAAGTGCAGATAATATGAAGGAGATATGTCATGGTCAGCTTCCAATGAGGTCATTTGAAAAACTAATCCAGATATCTTTAAGCAATCTGTCTCAGTTGACACATGTATGGAAGATAGAAGCTCAGTTTGAATGCCTGGGAAATCTAAGACGTGTCATTGTGAAATCCTGTTCAGCTTTGAAGTTTGTCTTCCTGTTGTCTACTGGTAATGATCTTAAGCAACTTGAAGAACTAGAGATTGCATCTTGCCCGTCCTTGGAAGATATTTTTACCTTGGAAGGATCAGGACATCAGATCAAGTGCCTGAATTTGATACGATTGCGTCTTGAAGATCTAGGGCTTAAGGTAAGCAAAAACACATGCATGATGAACTTCCCTCAACTAGTCACCTTGGAGCTCGAGTTCCTACCAGAACTCATCAGTCTCTATCCGAGTCATTTGGCTTCAAAACACAGCTCTAATGCCACTAATGTTCTATTTGATCCCAAGGTTAAACCTTTTCGTGAGCTACGGTATATGGTGGTGAGAAGATGTAACAATCTACTTGGTGTTTTCTCATCCAGTGTGGTGCAAAACTTACAGAATCTTGAACAACTTGAAGTAAGGTCTTGTGACTCACTGGAAACAGTATTTGAGCTTGAAGGTTTAGAAATGGAAGAAAAGGATGGTGTTACTGCAACACTATTGTCTTGCTTGGAGAGACTGGAGTTGGGTAATCTACCCAAGTTGGCATACTTGTGGAAGCCACCTCAAAACATTCTGGCATTTCAAAACTTAACAATGCTCTATGTTTTGCGGTGCAACAGATTGGAATATGTGTTCCCGCTCTTCATAGTGGAAGGTCTAGTGAAACTAGTAGCCATGTATATCAGAGATTGCCCTCAGATAGTAGACATCTTTGCAAAAACTGAAGAACATGGCGATGAAGAGAAAACAAATGAAATTGTTATTCCTCAGGTAAGAACTCTCCATCTTGCCGATTTACGTAGACTCAAAAGATTTTGTCCTGTTCTATGCACTGTTGACTGCCCATCATTGAAAACGCTGGCGCTCACCAACTGCGGTTACCTGGAGACATTTGTTCCACCGGTCGTAGCCACAGGAAGTGACCTGATCTACCTTTTTGGTGAACAGGTTGACTTTCCAGTTTTGGAGACACTAGAACTTCGTTACATGTGCCACTTCAAAGAGATATGGAAGAACAAACTTCCACCCCACTCCTTTTGTGAACTAACAGATCTAAACGTGTCCAATTGTACGGAATTATTGCATTTGGTGCCAATGTTCATGCAAAACAGGTTGGCGAAACTAGAGAAGTTAAATGTCAGTGACTGCAGGTCACTAGAAGAGATTTTTGAATTGAGAAATGCATCTGCCGATGAAGGGGAAGCTGTAATCATTTCACAGCCAGGGGAAACATCTTCAATTTCCCAACCTGAACGAATAATAAACCAGATGGATTACAGACTAACCCGAGGCTTTCCAAATTTGACATCAATAAAGGTTTCTGGTTGTAACAATTTGAGAAATCTGCTCTCTCTCTCCATTGCCAGAGGTCTGGTGAAGCTCAAAAATCTATCCATAGATCACTGCCAGAAGATGGAAGAAATCATAGCAGCAGATGGCGAGGAAACAGAGGACACGGTTGTTTTCCCAATCCTGGAGACACTAGAAATTCACGACATGGACAACTTCAAGGAGATATTCAGCAAACAATTTTCACACAATTCCTTCTGTAAACTAAGAGTACTCAGGGTGTCCTATTGTAACAAGTTAGAGCGTTTGCTTCCAATTTATATGCAAAATGGATTGCAGAAACTGGAGAACTTGACTGTGGAATATTGTTCATTACTAGAAGAGATTTTTGAATATAGTCAGTCGACTGGCAATGAGCATGCTGTCGTAGTATCCCAATCTGTAGGGATACAGAGTCATCCAGGCTTTCAGAGTCTAACTTCTATAGAAATCACAGAATGTGACAGACTGAGAATTCTGCTCTCACCTCGCATTGCCAGAGGTCTTAAGAATCTCAAAACTATAGATATAAGCCTTTGCAAGGAGTTGGAAGAAATAGTAACAAAGGAGGTTGAAGAAACGGATATGAGAAACATTCTCCCTCAGCTAACTTGTTTGAAACTTGTCCGTCTATATAATCTCAGTAGTTTTTCCCAAGGCAAATATGCTTTAGAATGGCCTTTAATGGAATTGATACTGATCAGGATGTGCACCAAGATGAAAAAATTCTGTTTTGGCTCCCTCAGCACACCAAGGGAAGTGGAATTAAGTATCAGTGAATACATTGATGTGAATCTTCGGTGGGAGCTCGAAAACAGCAGGAAACAAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF679

Protein Analysis

1849

Amino Acids

208.98

Weight (kDa)

5.72

Isoelectric Point (pI)

44.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 193 - 331 3.2e-13 NB-ARC domain
NB-ARC PF00931 355 - 512 7.8e-24 NB-ARC domain
AAA_22 PF13401 370 - 456 2.4e-06 AAA domain
LRR_RPS2 PF23247 1008 - 1150 4.6e-18 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1232 - 1360 3.1e-33 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1452 - 1599 6.5e-28 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1622 - 1701 1e-14 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1714 - 1788 6.6e-08 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 4527
AccB1I GGYRCC 1 cut(s) 4477
AccB7I CCANNNNNTGG 3 cut(s) 1811, 3167, 4801
AccBSI CCGCTC 2 cut(s) 56, 4027
AccI GTMKAC 4 cut(s) 3401, 4092, 4189, 5081
AccII CGCG 1 cut(s) 1992
AciI CCGC 9 cut(s) 54, 630, 748, 1122, 1667, 1990, 3997, 4025, 4263
AclI AACGTT 1 cut(s) 2294
AclWI GGATC 3 cut(s) 728, 3490, 3677
AcoI YGGCCR 3 cut(s) 129, 474, 2829
AcuI CTGAAG 4 cut(s) 219, 2081, 3026, 4131
AdeI CACNNNGTG 1 cut(s) 2492
AfaI GTAC 5 cut(s) 41, 2099, 2110, 4459, 4950
AflII CTTAAG 4 cut(s) 2219, 3416, 3542, 5222
AflIII ACRYGT 8 cut(s) 251, 2100, 2529, 2572, 3304, 3352, 4374, 4446
AgeI ACCGGT 1 cut(s) 4288
AhdI GACNNNNNGTC 1 cut(s) 4537
AhlI ACTAGT 5 cut(s) 2590, 2663, 2735, 3583, 4054
AjiI CACGTC 2 cut(s) 252, 3353
AjuI GAANNNNNNNTTGG 2 cut(s) 1597, 1629
AloI GAACNNNNNNTCC 4 cut(s) 3587, 3619, 4004, 4036
Alw21I GWGCWC 4 cut(s) 2243, 3600, 5429, 5526
Alw44I GTGCAC 1 cut(s) 5425
AlwI GGATC 3 cut(s) 728, 3490, 3677
AlwNI CAGNNNCTG 3 cut(s) 1582, 4533, 5020
Ama87I CYCGRG 3 cut(s) 3044, 3599, 4674
AoxI GGCC 5 cut(s) 129, 474, 1186, 2829, 5393
ApaLI GTGCAC 1 cut(s) 5425
ApeKI GCWGC 3 cut(s) 214, 2747, 4817
ArsI GACNNNNNNTTYG 6 cut(s) 371, 403, 2541, 2573, 3077, 3109
AseI ATTAAT 1 cut(s) 2079
AsiGI ACCGGT 1 cut(s) 4288
Asp700I GAANNNNTTC 1 cut(s) 5441
AspLEI GCGC 1 cut(s) 4252
AspS9I GGNCC 3 cut(s) 1034, 1987, 2557
AsuNHI GCTAGC 1 cut(s) 154
AvaI CYCGRG 3 cut(s) 3044, 3599, 4674
AvaII GGWCC 3 cut(s) 1034, 1987, 2557
AxyI CCTNAGG 2 cut(s) 502, 4155
BaeGI GKGCMC 2 cut(s) 1633, 5429
BalI TGGCCA 1 cut(s) 476
BanI GGYRCC 1 cut(s) 4477
BanII GRGCYC 2 cut(s) 3600, 5526
BbsI GAAGAC 1 cut(s) 3382
Bbv12I GWGCWC 4 cut(s) 2243, 3600, 5429, 5526
BbvCI CCTCAGC 2 cut(s) 5319, 5459
BbvI GCAGC 3 cut(s) 201, 2734, 4829
BceAI ACGGC 1 cut(s) 1545
BcgI CGANNNNNNTGC 2 cut(s) 1184, 1218
BciVI GTATCC 4 cut(s) 141, 744, 5118, 5119
BclI TGATCA 1 cut(s) 5415
BcuI ACTAGT 5 cut(s) 2590, 2663, 2735, 3583, 4054
BfmI CTRYAG 7 cut(s) 270, 1566, 2817, 4534, 5118, 5160, 5238
BfoI RGCGCY 1 cut(s) 4253
BfrI CTTAAG 4 cut(s) 2219, 3416, 3542, 5222
BfuAI ACCTGC 1 cut(s) 4527
BfuI GTATCC 4 cut(s) 141, 744, 5118, 5119
BglII AGATCT 4 cut(s) 2215, 3025, 3532, 4438
BisI GCNGC 3 cut(s) 215, 2748, 4818
BlsI GCNGC 3 cut(s) 216, 2749, 4819
BmcAI AGTACT 1 cut(s) 4950
Bme18I GGWCC 3 cut(s) 1034, 1987, 2557
BmeRI GACNNNNNGTC 1 cut(s) 4537
BmeT110I CYCGRG 3 cut(s) 3044, 3599, 4674
BmgBI CACGTC 2 cut(s) 252, 3353
BmgT120I GGNCC 3 cut(s) 1034, 1987, 2557
BmiI GGNNCC 7 cut(s) 508, 857, 1036, 2184, 2640, 4479, 5455
BmsI GCATC 9 cut(s) 810, 926, 1025, 1654, 2409, 2598, 3040, 3452, 4580
BmtI GCTAGC 1 cut(s) 158
BoxI GACNNNNGTC 1 cut(s) 1236
BpiI GAAGAC 1 cut(s) 3382
BpmI CTGGAG 6 cut(s) 1536, 2324, 3926, 4292, 4883, 5042
Bpu10I CCTNAGC 3 cut(s) 2441, 5319, 5459
BpuEI CTTGAG 7 cut(s) 349, 1255, 1910, 2495, 2743, 2896, 2968
BsaAI YACGTR 2 cut(s) 3164, 4187
BsaBI GATNNNNATC 2 cut(s) 5414, 5508
BsaWI WCCGGW 2 cut(s) 3178, 4288
BsaXI ACNNNNNCTCC 6 cut(s) 178, 208, 639, 669, 3587, 3617
Bse118I RCCGGY 2 cut(s) 2641, 4288
Bse1I ACTGG 9 cut(s) 1376, 1827, 3409, 3762, 3825, 3909, 4346, 5025, 5089
Bse21I CCTNAGG 2 cut(s) 502, 4155
Bse3DI GCAATG 5 cut(s) 1212, 2308, 4746, 5095, 5208
Bse8I GATNNNNATC 2 cut(s) 5414, 5508
BseJI GATNNNNATC 2 cut(s) 5414, 5508
BseMI GCAATG 5 cut(s) 1212, 2308, 4746, 5095, 5208
BseNI ACTGG 9 cut(s) 1376, 1827, 3409, 3762, 3825, 3909, 4346, 5025, 5089
BseRI GAGGAG 7 cut(s) 402, 490, 513, 660, 719, 2251, 2651
BseSI GKGCMC 2 cut(s) 1633, 5429
BseXI GCAGC 3 cut(s) 201, 2734, 4829
BseYI CCCAGC 1 cut(s) 119
BsgI GTGCAG 2 cut(s) 2125, 3228
Bsh1236I CGCG 1 cut(s) 1992
Bsh1285I CGRYCG 1 cut(s) 4293
BshFI GGCC 5 cut(s) 131, 476, 1188, 2831, 5395
BshNI GGYRCC 1 cut(s) 4477
BshTI ACCGGT 1 cut(s) 4288
BsiEI CGRYCG 1 cut(s) 4293
BsiHKAI GWGCWC 4 cut(s) 2243, 3600, 5429, 5526
BsiHKCI CYCGRG 3 cut(s) 3044, 3599, 4674
BsiSI CCGG 3 cut(s) 2642, 3179, 4289
BslFI GGGAC 1 cut(s) 712
BsmBI CGTCTC 3 cut(s) 242, 372, 1520
BsmFI GGGAC 1 cut(s) 712
BsmI GAATGC 5 cut(s) 595, 1087, 1131, 1980, 3338
BsnI GGCC 5 cut(s) 131, 476, 1188, 2831, 5395
BsoBI CYCGRG 3 cut(s) 3044, 3599, 4674
Bsp1286I GDGCHC 5 cut(s) 1633, 2243, 3600, 5429, 5526
Bsp19I CCATGG 2 cut(s) 1038, 2826
BspACI CCGC 9 cut(s) 54, 630, 748, 1122, 1667, 1990, 3997, 4025, 4263
BspANI GGCC 5 cut(s) 131, 476, 1188, 2831, 5395
BspFNI CGCG 1 cut(s) 1992
BspHI TCATGA 3 cut(s) 585, 2884, 2956
BspLI GGNNCC 7 cut(s) 508, 857, 1036, 2184, 2640, 4479, 5455
BspMAI CTGCAG 1 cut(s) 4538
BspMI ACCTGC 1 cut(s) 4527
BspOI GCTAGC 1 cut(s) 158
BspPI GGATC 3 cut(s) 728, 3490, 3677
BspQI GCTCTTC 2 cut(s) 1297, 4034
BspT107I GGYRCC 1 cut(s) 4477
BspTI CTTAAG 4 cut(s) 2219, 3416, 3542, 5222
BsrBI CCGCTC 2 cut(s) 56, 4027
BsrDI GCAATG 5 cut(s) 1212, 2308, 4746, 5095, 5208
BsrFI RCCGGY 2 cut(s) 2641, 4288
BsrI ACTGG 9 cut(s) 1376, 1827, 3409, 3762, 3825, 3909, 4346, 5025, 5089
BssAI RCCGGY 2 cut(s) 2641, 4288
Bst6I CTCTTC 5 cut(s) 1297, 4034, 4122, 4542, 5052
BstAFI CTTAAG 4 cut(s) 2219, 3416, 3542, 5222
BstAPI GCANNNNNTGC 3 cut(s) 2143, 2608, 4477
BstBAI YACGTR 2 cut(s) 3164, 4187
BstC8I GCNNGC 7 cut(s) 129, 156, 496, 1534, 1649, 4248, 5097
BstDSI CCRYGG 2 cut(s) 1038, 2826
BstEII GGTNACC 1 cut(s) 4265
BstENI CCTNNNNNAGG 3 cut(s) 331, 1134, 5255
BstFNI CGCG 1 cut(s) 1992
BstH2I RGCGCY 1 cut(s) 4253
BstHHI GCGC 1 cut(s) 4252
BstMCI CGRYCG 1 cut(s) 4293
BstMWI GCNNNNNNNGC 9 cut(s) 2143, 2238, 2260, 2391, 2446, 2608, 2638, 4477, 5460
BstNSI RCATGY 8 cut(s) 288, 2104, 2533, 2576, 3308, 3564, 4378, 5099
BstPAI GACNNNNGTC 1 cut(s) 1236
BstPI GGTNACC 1 cut(s) 4265
BstSFI CTRYAG 7 cut(s) 270, 1566, 2817, 4534, 5118, 5160, 5238
BstSLI GKGCMC 2 cut(s) 1633, 5429
BstSNI TACGTA 1 cut(s) 4187
BstUI CGCG 1 cut(s) 1992
BstV1I GCAGC 3 cut(s) 201, 2734, 4829
BstV2I GAAGAC 1 cut(s) 3382
BstX2I RGATCY 4 cut(s) 2215, 3025, 3532, 4438
BstXI CCANNNNNNTGG 1 cut(s) 4759
BstYI RGATCY 4 cut(s) 2215, 3025, 3532, 4438
Bsu36I CCTNAGG 2 cut(s) 502, 4155
BsuI GTATCC 4 cut(s) 141, 744, 5118, 5119
BsuRI GGCC 5 cut(s) 131, 476, 1188, 2831, 5395
BtgI CCRYGG 2 cut(s) 1038, 2826
BtgZI GCGATG 3 cut(s) 2007, 2250, 4137
BtrI CACGTC 2 cut(s) 252, 3353
BtsI GCAGTG 4 cut(s) 1631, 2785, 3101, 4789
BveI ACCTGC 1 cut(s) 4527
Cac8I GCNNGC 7 cut(s) 129, 156, 496, 1534, 1649, 4248, 5097
CaiI CAGNNNCTG 3 cut(s) 1582, 4533, 5020
CciI TCATGA 3 cut(s) 585, 2884, 2956
CfoI GCGC 1 cut(s) 4252
Cfr10I RCCGGY 2 cut(s) 2641, 4288
Cfr13I GGNCC 3 cut(s) 1034, 1987, 2557
CseI GACGC 1 cut(s) 3512
CsiI ACCWGGT 1 cut(s) 2488
Csp6I GTAC 5 cut(s) 40, 2098, 2109, 4458, 4949
CspAI ACCGGT 1 cut(s) 4288
CspCI CAANNNNNGTGG 6 cut(s) 2182, 2217, 4406, 4441, 5457, 5492
CviQI GTAC 5 cut(s) 40, 2098, 2109, 4458, 4949
DraI TTTAAA 1 cut(s) 2809
DraIII CACNNNGTG 1 cut(s) 2492
DriI GACNNNNNGTC 1 cut(s) 4537
EaeI YGGCCR 3 cut(s) 129, 474, 2829
Eam1104I CTCTTC 5 cut(s) 1297, 4034, 4122, 4542, 5052
Eam1105I GACNNNNNGTC 1 cut(s) 4537
EarI CTCTTC 5 cut(s) 1297, 4034, 4122, 4542, 5052
Ecl136II GAGCTC 2 cut(s) 3598, 5524
Eco105I TACGTA 1 cut(s) 4187
Eco24I GRGCYC 2 cut(s) 3600, 5526
Eco32I GATATC 2 cut(s) 610, 3276
Eco47I GGWCC 3 cut(s) 1034, 1987, 2557
Eco53kI GAGCTC 2 cut(s) 3598, 5524
Eco57I CTGAAG 4 cut(s) 219, 2081, 3026, 4131
Eco81I CCTNAGG 2 cut(s) 502, 4155
Eco88I CYCGRG 3 cut(s) 3044, 3599, 4674
Eco91I GGTNACC 1 cut(s) 4265
EcoICRI GAGCTC 2 cut(s) 3598, 5524
EcoNI CCTNNNNNAGG 3 cut(s) 331, 1134, 5255
EcoO109I RGGNCCY 1 cut(s) 1034
EcoO65I GGTNACC 1 cut(s) 4265
EcoRI GAATTC 3 cut(s) 668, 2952, 5190
EcoRV GATATC 2 cut(s) 610, 3276
EcoT22I ATGCAT 3 cut(s) 1980, 3566, 4573
EcoT38I GRGCYC 2 cut(s) 3600, 5526
Esp3I CGTCTC 3 cut(s) 242, 372, 1520
FalI AAGNNNNNCTT 6 cut(s) 1133, 1165, 1672, 1704, 3374, 3406
FaqI GGGAC 1 cut(s) 712
FauI CCCGC 3 cut(s) 61, 623, 4032
FbaI TGATCA 1 cut(s) 5415
FblI GTMKAC 4 cut(s) 3401, 4092, 4189, 5081
Fnu4HI GCNGC 3 cut(s) 215, 2748, 4818
FriOI GRGCYC 2 cut(s) 3600, 5526
Fsp4HI GCNGC 3 cut(s) 215, 2748, 4818
GlaI GCGC 1 cut(s) 4251
GluI GCNGC 3 cut(s) 215, 2748, 4818
GsaI CCCAGC 1 cut(s) 123
GsuI CTGGAG 6 cut(s) 1536, 2324, 3926, 4292, 4883, 5042
HaeII RGCGCY 1 cut(s) 4253
HaeIII GGCC 5 cut(s) 131, 476, 1188, 2831, 5395
HapII CCGG 3 cut(s) 2642, 3179, 4289
HgaI GACGC 1 cut(s) 3512
HhaI GCGC 1 cut(s) 4252
Hin6I GCGC 1 cut(s) 4250
HinP1I GCGC 1 cut(s) 4250
HincII GTYRAC 6 cut(s) 226, 2661, 3300, 4225, 4339, 5082
HindII GTYRAC 6 cut(s) 226, 2661, 3300, 4225, 4339, 5082
HindIII AAGCTT 1 cut(s) 2795
HpaI GTTAAC 1 cut(s) 226
HpaII CCGG 3 cut(s) 2642, 3179, 4289
HpyCH4IV ACGT 6 cut(s) 251, 2294, 3163, 3352, 4186, 4446
HpyF10VI GCNNNNNNNGC 9 cut(s) 2143, 2238, 2260, 2391, 2446, 2608, 2638, 4477, 5460
HpySE526I ACGT 6 cut(s) 251, 2294, 3163, 3352, 4186, 4446
HspAI GCGC 1 cut(s) 4250
Ksp22I TGATCA 1 cut(s) 5415
KspAI GTTAAC 1 cut(s) 226
LguI GCTCTTC 2 cut(s) 1297, 4034
Lsp1109I GCAGC 3 cut(s) 201, 2734, 4829
LweI GCATC 9 cut(s) 810, 926, 1025, 1654, 2409, 2598, 3040, 3452, 4580
MabI ACCWGGT 1 cut(s) 2488
MaeII ACGT 6 cut(s) 251, 2294, 3163, 3352, 4186, 4446
MbiI CCGCTC 2 cut(s) 56, 4027
MfeI CAATTG 3 cut(s) 1110, 1605, 4453
MflI RGATCY 4 cut(s) 2215, 3025, 3532, 4438
MhlI GDGCHC 5 cut(s) 1633, 2243, 3600, 5429, 5526
MlsI TGGCCA 1 cut(s) 476
MluNI TGGCCA 1 cut(s) 476
MlyI GAGTC 8 cut(s) 215, 1246, 2666, 3643, 3809, 4185, 5140, 5158
MmeI TCCRAC 4 cut(s) 537, 886, 1603, 5244
Mox20I TGGCCA 1 cut(s) 476
Mph1103I ATGCAT 3 cut(s) 1980, 3566, 4573
MroXI GAANNNNTTC 1 cut(s) 5441
MscI TGGCCA 1 cut(s) 476
MslI CAYNNNNRTG 8 cut(s) 603, 3137, 3307, 3563, 3937, 4122, 5432, 5504
Msp20I TGGCCA 1 cut(s) 476
MspCI CTTAAG 4 cut(s) 2219, 3416, 3542, 5222
MspI CCGG 3 cut(s) 2642, 3179, 4289
MunI CAATTG 3 cut(s) 1110, 1605, 4453
Mva1269I GAATGC 5 cut(s) 595, 1087, 1131, 1980, 3338
MvnI CGCG 1 cut(s) 1992
MwoI GCNNNNNNNGC 9 cut(s) 2143, 2238, 2260, 2391, 2446, 2608, 2638, 4477, 5460
NcoI CCATGG 2 cut(s) 1038, 2826
NheI GCTAGC 1 cut(s) 154
NlaIV GGNNCC 7 cut(s) 508, 857, 1036, 2184, 2640, 4479, 5455
NmuCI GTSAC 7 cut(s) 655, 3586, 3812, 4307, 4529, 4539, 5177
NsiI ATGCAT 3 cut(s) 1980, 3566, 4573
NspI RCATGY 8 cut(s) 288, 2104, 2533, 2576, 3308, 3564, 4378, 5099
PaeI GCATGC 1 cut(s) 5099
PaeR7I CTCGAG 2 cut(s) 3044, 3599
PagI TCATGA 3 cut(s) 585, 2884, 2956
PciI ACATGT 5 cut(s) 2100, 2529, 2572, 3304, 4374
PciSI GCTCTTC 2 cut(s) 1297, 4034
PctI GAATGC 5 cut(s) 595, 1087, 1131, 1980, 3338
PdmI GAANNNNTTC 1 cut(s) 5441
PflMI CCANNNNNTGG 3 cut(s) 1811, 3167, 4801
PfoI TCCNGGA 2 cut(s) 1705, 4860
PinAI ACCGGT 1 cut(s) 4288
PkrI GCNGC 3 cut(s) 216, 2749, 4819
PleI GAGTC 8 cut(s) 214, 1245, 2665, 3642, 3809, 4185, 5139, 5157
PpsI GAGTC 8 cut(s) 214, 1245, 2665, 3642, 3809, 4185, 5139, 5157
Ppu21I YACGTR 2 cut(s) 3164, 4187
PpuMI RGGWCCY 1 cut(s) 1034
PscI ACATGT 5 cut(s) 2100, 2529, 2572, 3304, 4374
PshAI GACNNNNGTC 1 cut(s) 1236
PshBI ATTAAT 1 cut(s) 2079
Psp124BI GAGCTC 2 cut(s) 3600, 5526
Psp1406I AACGTT 1 cut(s) 2294
Psp5II RGGWCCY 1 cut(s) 1034
PspEI GGTNACC 1 cut(s) 4265
PspFI CCCAGC 1 cut(s) 119
PspN4I GGNNCC 7 cut(s) 508, 857, 1036, 2184, 2640, 4479, 5455
PspPI GGNCC 3 cut(s) 1034, 1987, 2557
PspPPI RGGWCCY 1 cut(s) 1034
PspXI VCTCGAGB 1 cut(s) 3599
PstI CTGCAG 1 cut(s) 4538
PstNI CAGNNNCTG 3 cut(s) 1582, 4533, 5020
PsuI RGATCY 4 cut(s) 2215, 3025, 3532, 4438
RsaI GTAC 5 cut(s) 41, 2099, 2110, 4459, 4950
RsaNI GTAC 5 cut(s) 40, 2098, 2109, 4458, 4949
RseI CAYNNNNRTG 8 cut(s) 603, 3137, 3307, 3563, 3937, 4122, 5432, 5504
SacI GAGCTC 2 cut(s) 3600, 5526
SalI GTCGAC 1 cut(s) 5080
SapI GCTCTTC 2 cut(s) 1297, 4034
SatI GCNGC 3 cut(s) 215, 2748, 4818
Sau96I GGNCC 3 cut(s) 1034, 1987, 2557
ScaI AGTACT 1 cut(s) 4950
SchI GAGTC 8 cut(s) 215, 1246, 2666, 3643, 3809, 4185, 5140, 5158
SduI GDGCHC 5 cut(s) 1633, 2243, 3600, 5429, 5526
SexAI ACCWGGT 1 cut(s) 2488
SfaNI GCATC 9 cut(s) 810, 926, 1025, 1654, 2409, 2598, 3040, 3452, 4580
SfcI CTRYAG 7 cut(s) 270, 1566, 2817, 4534, 5118, 5160, 5238
Sfr274I CTCGAG 2 cut(s) 3044, 3599
SinI GGWCC 3 cut(s) 1034, 1987, 2557
SlaI CTCGAG 2 cut(s) 3044, 3599
SmiMI CAYNNNNRTG 8 cut(s) 603, 3137, 3307, 3563, 3937, 4122, 5432, 5504
SnaBI TACGTA 1 cut(s) 4187
SpeI ACTAGT 5 cut(s) 2590, 2663, 2735, 3583, 4054
SphI GCATGC 1 cut(s) 5099
SsiI CCGC 9 cut(s) 54, 630, 748, 1122, 1667, 1990, 3997, 4025, 4263
SspI AATATT 3 cut(s) 1356, 1449, 5042
SstI GAGCTC 2 cut(s) 3600, 5526
TaiI ACGT 6 cut(s) 254, 2297, 3166, 3355, 4189, 4449
TaqI TCGA 7 cut(s) 759, 1194, 2435, 3045, 3600, 5081, 5526
TatI WGTACW 4 cut(s) 39, 2097, 2108, 4948
TseFI GTSAC 7 cut(s) 655, 3586, 3812, 4307, 4529, 4539, 5177
TseI GCWGC 3 cut(s) 214, 2747, 4817
Tsp45I GTSAC 7 cut(s) 655, 3586, 3812, 4307, 4529, 4539, 5177
TspGWI ACGGA 5 cut(s) 2380, 3158, 4475, 5312, 5333
Van91I CCANNNNNTGG 3 cut(s) 1811, 3167, 4801
Vha464I CTTAAG 4 cut(s) 2219, 3416, 3542, 5222
VneI GTGCAC 1 cut(s) 5425
VpaK11BI GGWCC 3 cut(s) 1034, 1987, 2557
VspI ATTAAT 1 cut(s) 2079
XagI CCTNNNNNAGG 3 cut(s) 331, 1134, 5255
XceI RCATGY 8 cut(s) 288, 2104, 2533, 2576, 3308, 3564, 4378, 5099
XhoI CTCGAG 2 cut(s) 3044, 3599
XmiI GTMKAC 4 cut(s) 3401, 4092, 4189, 5081
XmnI GAANNNNTTC 1 cut(s) 5441
ZrmI AGTACT 1 cut(s) 4950
Zsp2I ATGCAT 3 cut(s) 1980, 3566, 4573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.