Rh4AG223600

Protein of unknown function (DUF679)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0003054
Physical Location & Seq
Reverse (-)
52318695 .. 52319849
1155 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG223600.1

Sequence Viewer

Length: 603 bp
ATGGGGGCTTCATCTAGCGCTTCATCCAAATCCAAGAAATCCACCAAAAGCAAGGGTGTCAAAGACATTGCATTAACAGGTGTAGGCAACCTCATCAAGCTCCTCCCTACCGGCACGGTATTCTTGTTCCAGTTCCTCAACCCCGTTTTAACCAACAATGGCCAATGCAACCTCCCCGTCAACAAGATTCTAAGCGCCATTCTCATTGTCGTTTCCGCCTTCTCTTGCTGCTTTGCTTCCTTCACCGACAGTTACACCGGCAGTGATGGACAAACTCACTATGGAATTGCAACAACAAAGGGTCTTTGGCCCTCAACCAGTTCTGGCTCTGTGGATTTGTCTGCTTACAAGCTTCGGGTTGGGGACTTTGTGCATACCTTCTTTTCCTTGATTGTGTTTGCAGTGGTATCACTATTGGATTCCAACACTGTGAGGTGCTTCTATCCTGCGTTTGAGTCCAGTGGGAGGATCTGGCTCATGGTGTTGCCTCCTGTGATTGGCACTATTGCTGGTACTGTCTTTGTTGTGTTCCCTAATAAACGCCACGGAATTGGGTACCCTTCTTCAAGTTCAGATTCTTCAGAAGACTCTGAGGACTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF679

Protein Analysis

200

Amino Acids

21.2

Weight (kDa)

8.68

Isoelectric Point (pI)

41.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF679 PF05078 27 - 186 1.1e-61 Protein of unknown function (DUF679)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 555
AccB1I GGYRCC 1 cut(s) 555
AciI CCGC 1 cut(s) 216
AclWI GGATC 1 cut(s) 476
AcoI YGGCCR 1 cut(s) 160
AcuI CTGAAG 1 cut(s) 564
AfaI GTAC 2 cut(s) 514, 557
AfeI AGCGCT 1 cut(s) 19
AfiI CCNNNNNNNGG 2 cut(s) 465, 497
AgsI TTSAA 1 cut(s) 567
AluBI AGCT 2 cut(s) 100, 352
AluI AGCT 2 cut(s) 100, 352
AlwI GGATC 1 cut(s) 476
Aor51HI AGCGCT 1 cut(s) 19
AoxI GGCC 2 cut(s) 160, 308
ApeKI GCWGC 1 cut(s) 228
Asp718I GGTACC 1 cut(s) 555
AspLEI GCGC 2 cut(s) 20, 197
AspS9I GGNCC 1 cut(s) 309
AsuHPI GGTGA 1 cut(s) 235
BalI TGGCCA 1 cut(s) 162
BanI GGYRCC 1 cut(s) 555
BbsI GAAGAC 1 cut(s) 591
BbvI GCAGC 1 cut(s) 215
BccI CCATC 1 cut(s) 260
BfaI CTAG 2 cut(s) 15, 601
BfoI RGCGCY 2 cut(s) 21, 198
BisI GCNGC 1 cut(s) 229
BlsI GCNGC 1 cut(s) 230
BmgT120I GGNCC 1 cut(s) 309
BmiI GGNNCC 1 cut(s) 557
BpiI GAAGAC 1 cut(s) 591
BsaJI CCNNGG 1 cut(s) 544
Bsc4I CCNNNNNNNGG 2 cut(s) 465, 497
Bse118I RCCGGY 2 cut(s) 110, 257
Bse1I ACTGG 3 cut(s) 130, 318, 459
Bse3DI GCAATG 1 cut(s) 66
BseDI CCNNGG 1 cut(s) 544
BseGI GGATG 1 cut(s) 23
BseLI CCNNNNNNNGG 2 cut(s) 465, 497
BseMI GCAATG 1 cut(s) 66
BseMII CTCAG 1 cut(s) 582
BseNI ACTGG 3 cut(s) 130, 318, 459
BseRI GAGGAG 1 cut(s) 92
BseXI GCAGC 1 cut(s) 215
BshFI GGCC 2 cut(s) 162, 310
BshNI GGYRCC 1 cut(s) 555
BsiSI CCGG 2 cut(s) 111, 258
BslFI GGGAC 1 cut(s) 377
BslI CCNNNNNNNGG 2 cut(s) 465, 497
BsmFI GGGAC 1 cut(s) 377
BsnI GGCC 2 cut(s) 162, 310
Bsp143I GATC 1 cut(s) 468
BspACI CCGC 1 cut(s) 216
BspANI GGCC 2 cut(s) 162, 310
BspCNI CTCAG 1 cut(s) 583
BspLI GGNNCC 1 cut(s) 557
BspPI GGATC 1 cut(s) 476
BspT107I GGYRCC 1 cut(s) 555
BsrDI GCAATG 1 cut(s) 66
BsrFI RCCGGY 2 cut(s) 110, 257
BsrI ACTGG 3 cut(s) 130, 318, 459
BssAI RCCGGY 2 cut(s) 110, 257
BssECI CCNNGG 1 cut(s) 544
BssMI GATC 1 cut(s) 468
Bst4CI ACNGT 4 cut(s) 118, 251, 430, 517
BstDEI CTNAG 2 cut(s) 191, 591
BstDSI CCRYGG 1 cut(s) 544
BstF5I GGATG 1 cut(s) 23
BstH2I RGCGCY 2 cut(s) 21, 198
BstHHI GCGC 2 cut(s) 20, 197
BstKTI GATC 1 cut(s) 471
BstMBI GATC 1 cut(s) 468
BstV1I GCAGC 1 cut(s) 215
BstV2I GAAGAC 1 cut(s) 591
BstX2I RGATCY 1 cut(s) 468
BstXI CCANNNNNNTGG 1 cut(s) 551
BstYI RGATCY 1 cut(s) 468
BsuRI GGCC 2 cut(s) 162, 310
BtgI CCRYGG 1 cut(s) 544
BtsCI GGATG 1 cut(s) 23
BtsI GCAGTG 2 cut(s) 268, 408
BtsIMutI CAGTG 4 cut(s) 268, 408, 426, 466
CfoI GCGC 2 cut(s) 20, 197
Cfr10I RCCGGY 2 cut(s) 110, 257
Cfr13I GGNCC 1 cut(s) 309
Csp6I GTAC 2 cut(s) 513, 556
CviAII CATG 1 cut(s) 478
CviJI RGCY 7 cut(s) 8, 100, 162, 310, 327, 352, 475
CviKI_1 RGCY 7 cut(s) 8, 100, 162, 310, 327, 352, 475
CviQI GTAC 2 cut(s) 513, 556
DdeI CTNAG 2 cut(s) 191, 591
DpnI GATC 1 cut(s) 470
DpnII GATC 1 cut(s) 468
EaeI YGGCCR 1 cut(s) 160
EciI GGCGGA 1 cut(s) 205
Eco47III AGCGCT 1 cut(s) 19
Eco57I CTGAAG 1 cut(s) 564
FaeI CATG 1 cut(s) 481
FaiI YATR 3 cut(s) 282, 375, 479
FaqI GGGAC 1 cut(s) 377
FatI CATG 1 cut(s) 477
Fnu4HI GCNGC 1 cut(s) 229
FokI GGATG 1 cut(s) 10
Fsp4HI GCNGC 1 cut(s) 229
FspBI CTAG 2 cut(s) 15, 601
GlaI GCGC 2 cut(s) 19, 196
GluI GCNGC 1 cut(s) 229
HaeII RGCGCY 2 cut(s) 21, 198
HaeIII GGCC 2 cut(s) 162, 310
HapII CCGG 2 cut(s) 111, 258
HhaI GCGC 2 cut(s) 20, 197
Hin1II CATG 1 cut(s) 481
Hin6I GCGC 2 cut(s) 18, 195
HinP1I GCGC 2 cut(s) 18, 195
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HindIII AAGCTT 1 cut(s) 350
HinfI GANTC 6 cut(s) 187, 419, 455, 575, 587, 596
HpaII CCGG 2 cut(s) 111, 258
HphI GGTGA 1 cut(s) 235
Hpy166II GTNNAC 1 cut(s) 181
Hpy188I TCNGA 3 cut(s) 574, 583, 592
Hpy8I GTNNAC 1 cut(s) 181
HpyAV CCTTC 4 cut(s) 229, 250, 388, 570
HpyCH4III ACNGT 4 cut(s) 118, 251, 430, 517
HpyCH4V TGCA 5 cut(s) 71, 168, 290, 373, 401
HpyF3I CTNAG 2 cut(s) 191, 591
Hsp92II CATG 1 cut(s) 481
HspAI GCGC 2 cut(s) 18, 195
KpnI GGTACC 1 cut(s) 559
Kzo9I GATC 1 cut(s) 468
LmnI GCTCC 1 cut(s) 105
Lsp1109I GCAGC 1 cut(s) 215
MaeI CTAG 2 cut(s) 15, 601
MaeIII GTNAC 1 cut(s) 251
MalI GATC 1 cut(s) 470
MboI GATC 1 cut(s) 468
MboII GAAGA 3 cut(s) 555, 570, 596
MflI RGATCY 1 cut(s) 468
MlsI TGGCCA 1 cut(s) 162
MluCI AATT 2 cut(s) 285, 549
MluNI TGGCCA 1 cut(s) 162
MlyI GAGTC 3 cut(s) 464, 581, 590
MmeI TCCRAC 1 cut(s) 447
MnlI CCTC 9 cut(s) 101, 113, 146, 182, 322, 426, 459, 498, 586
Mox20I TGGCCA 1 cut(s) 162
MscI TGGCCA 1 cut(s) 162
MseI TTAA 2 cut(s) 74, 149
Msp20I TGGCCA 1 cut(s) 162
MspI CCGG 2 cut(s) 111, 258
NdeII GATC 1 cut(s) 468
NlaIII CATG 1 cut(s) 481
NlaIV GGNNCC 1 cut(s) 557
PfeI GAWTC 3 cut(s) 187, 419, 575
PkrI GCNGC 1 cut(s) 230
PleI GAGTC 3 cut(s) 463, 581, 590
PpsI GAGTC 3 cut(s) 463, 581, 590
PspN4I GGNNCC 1 cut(s) 557
PspPI GGNCC 1 cut(s) 309
PsuI RGATCY 1 cut(s) 468
RsaI GTAC 2 cut(s) 514, 557
RsaNI GTAC 2 cut(s) 513, 556
SaqAI TTAA 2 cut(s) 74, 149
SatI GCNGC 1 cut(s) 229
Sau3AI GATC 1 cut(s) 468
Sau96I GGNCC 1 cut(s) 309
SchI GAGTC 3 cut(s) 464, 581, 590
SetI ASST 7 cut(s) 82, 93, 102, 174, 354, 380, 437
Sse9I AATT 2 cut(s) 285, 549
SsiI CCGC 1 cut(s) 216
SspMI CTAG 2 cut(s) 15, 601
TaaI ACNGT 4 cut(s) 118, 251, 430, 517
TasI AATT 2 cut(s) 285, 549
TfiI GAWTC 3 cut(s) 187, 419, 575
Tru1I TTAA 2 cut(s) 74, 149
Tru9I TTAA 2 cut(s) 74, 149
TscAI CASTG 4 cut(s) 268, 408, 433, 466
TseI GCWGC 1 cut(s) 228
TspDTI ATGAA 1 cut(s) 12
TspGWI ACGGA 1 cut(s) 561
TspRI CASTG 4 cut(s) 268, 408, 433, 466
XspI CTAG 2 cut(s) 15, 601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.