MD13G1177000.v1.1

Protein of unknown function (DUF679)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0003054
Physical Location & Seq
Forward (+)
14706625 .. 14707233
609 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1177000.v1.1.491

Sequence Viewer

Length: 609 bp
ATGGGGGGTTCTACTAGCTCTTCCTCTAAATCCAAGACAACCACAACCAAAAACAAAAGCATCAAAGACAGAACATTAACATGCGCAGGCAACCTCATCAAGCTCCTCCCAACCGGCACGGTCTTCTTGTTCCAGTTCCTCAACCCTGTCTTGACCAACAACGGCAAATGCACCGCTGTCAACAAATATCTAAGCGCCATTCTCATCGGTGTTTGCGGCTTCTCCTGCTGTTTTTCTTCCTTCACAGACAGTTACACAGGCAGTGATGGACAAACACACTATGGGGTGGTAACAAGCAAGGGGCTTTGGCCCTCGACAAGTTCCAACTCTGTGGACTTGTCTGCTTACAAGCTTAGGTTTGGGGACTTTGTGCATGCCTTCTTTTCCTTGATTGTGTTTGCAGTTGTGTCACTTTTGGATGCCAACACTGTCAGGTGCTATTATCCAGGGTTTGAGTCCACCGAGAAGATTTTGCTACAGGTGTTGCCTCCGGTTATAGGTACAATTGCCGGAACTGTTTTCTCTGTGTTCCCTAATAAACGCCATGGAATTGGGTACCCGTCTTCAAGTTCTGATTCTTCACAGGATTCGGAGTCGGCAGCTTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF679

Protein Analysis

203

Amino Acids

21.49

Weight (kDa)

8.89

Isoelectric Point (pI)

41.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF679 PF05078 29 - 186 2.8e-65 Protein of unknown function (DUF679)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 85
Acc65I GGTACC 1 cut(s) 555
AccB1I GGYRCC 1 cut(s) 555
AciI CCGC 2 cut(s) 174, 216
AfaI GTAC 2 cut(s) 502, 557
AfiI CCNNNNNNNGG 1 cut(s) 497
AgsI TTSAA 1 cut(s) 567
AjnI CCWGG 1 cut(s) 445
AluBI AGCT 4 cut(s) 18, 103, 352, 602
AluI AGCT 4 cut(s) 18, 103, 352, 602
AoxI GGCC 1 cut(s) 308
ApeKI GCWGC 1 cut(s) 599
Asp718I GGTACC 1 cut(s) 555
AspLEI GCGC 2 cut(s) 86, 197
AspS9I GGNCC 1 cut(s) 309
BanI GGYRCC 1 cut(s) 555
BbsI GAAGAC 2 cut(s) 115, 555
BccI CCATC 1 cut(s) 260
BceAI ACGGC 1 cut(s) 178
BciT130I CCWGG 1 cut(s) 447
BfaI CTAG 1 cut(s) 15
BfmI CTRYAG 1 cut(s) 476
BfoI RGCGCY 1 cut(s) 198
BisI GCNGC 2 cut(s) 217, 600
BlsI GCNGC 2 cut(s) 218, 601
Bme1390I CCNGG 1 cut(s) 447
BmgT120I GGNCC 1 cut(s) 309
BmiI GGNNCC 1 cut(s) 557
BmrFI CCNGG 1 cut(s) 447
BmsI GCATC 2 cut(s) 69, 409
BpiI GAAGAC 2 cut(s) 115, 555
Bpu10I CCTNAGC 1 cut(s) 353
BsaJI CCNNGG 2 cut(s) 446, 544
BsaWI WCCGGW 1 cut(s) 490
Bsc4I CCNNNNNNNGG 1 cut(s) 497
Bse118I RCCGGY 1 cut(s) 113
Bse1I ACTGG 1 cut(s) 133
BseBI CCWGG 1 cut(s) 447
BseDI CCNNGG 2 cut(s) 446, 544
BseGI GGATG 1 cut(s) 424
BseLI CCNNNNNNNGG 1 cut(s) 497
BseNI ACTGG 1 cut(s) 133
BseRI GAGGAG 1 cut(s) 95
BshFI GGCC 1 cut(s) 310
BshNI GGYRCC 1 cut(s) 555
BsiSI CCGG 3 cut(s) 114, 491, 510
BslFI GGGAC 1 cut(s) 377
BslI CCNNNNNNNGG 1 cut(s) 497
BsmFI GGGAC 1 cut(s) 377
BsnI GGCC 1 cut(s) 310
Bsp19I CCATGG 1 cut(s) 544
BspACI CCGC 2 cut(s) 174, 216
BspANI GGCC 1 cut(s) 310
BspLI GGNNCC 1 cut(s) 557
BspQI GCTCTTC 1 cut(s) 25
BspT107I GGYRCC 1 cut(s) 555
BsrFI RCCGGY 1 cut(s) 113
BsrI ACTGG 1 cut(s) 133
BssAI RCCGGY 1 cut(s) 113
BssECI CCNNGG 2 cut(s) 446, 544
BssT1I CCWWGG 1 cut(s) 544
Bst2UI CCWGG 1 cut(s) 447
Bst4CI ACNGT 4 cut(s) 121, 251, 430, 517
Bst6I CTCTTC 1 cut(s) 25
BstC8I GCNNGC 2 cut(s) 88, 375
BstDEI CTNAG 2 cut(s) 191, 353
BstDSI CCRYGG 1 cut(s) 544
BstF5I GGATG 1 cut(s) 424
BstH2I RGCGCY 1 cut(s) 198
BstHHI GCGC 2 cut(s) 86, 197
BstMWI GCNNNNNNNGC 1 cut(s) 225
BstNI CCWGG 1 cut(s) 447
BstNSI RCATGY 2 cut(s) 84, 377
BstSCI CCNGG 1 cut(s) 445
BstSFI CTRYAG 1 cut(s) 476
BstV2I GAAGAC 2 cut(s) 115, 555
BstXI CCANNNNNNTGG 2 cut(s) 331, 551
BsuRI GGCC 1 cut(s) 310
BtgI CCRYGG 1 cut(s) 544
BtsCI GGATG 1 cut(s) 424
BtsI GCAGTG 1 cut(s) 268
BtsIMutI CAGTG 2 cut(s) 268, 426
Cac8I GCNNGC 2 cut(s) 88, 375
CfoI GCGC 2 cut(s) 86, 197
Cfr10I RCCGGY 1 cut(s) 113
Cfr13I GGNCC 1 cut(s) 309
Csp6I GTAC 2 cut(s) 501, 556
CviAII CATG 3 cut(s) 81, 374, 545
CviJI RGCY 7 cut(s) 18, 103, 219, 304, 310, 352, 602
CviKI_1 RGCY 7 cut(s) 18, 103, 219, 304, 310, 352, 602
CviQI GTAC 2 cut(s) 501, 556
DdeI CTNAG 2 cut(s) 191, 353
Eam1104I CTCTTC 1 cut(s) 25
EarI CTCTTC 1 cut(s) 25
Eco130I CCWWGG 1 cut(s) 544
EcoRII CCWGG 1 cut(s) 445
EcoT14I CCWWGG 1 cut(s) 544
ErhI CCWWGG 1 cut(s) 544
FaeI CATG 3 cut(s) 84, 377, 548
FaiI YATR 5 cut(s) 82, 282, 375, 497, 546
FaqI GGGAC 1 cut(s) 377
FatI CATG 3 cut(s) 80, 373, 544
Fnu4HI GCNGC 2 cut(s) 217, 600
FokI GGATG 1 cut(s) 431
Fsp4HI GCNGC 2 cut(s) 217, 600
FspBI CTAG 1 cut(s) 15
FspI TGCGCA 1 cut(s) 85
GlaI GCGC 2 cut(s) 85, 196
GluI GCNGC 2 cut(s) 217, 600
HaeII RGCGCY 1 cut(s) 198
HaeIII GGCC 1 cut(s) 310
HapII CCGG 3 cut(s) 114, 491, 510
HhaI GCGC 2 cut(s) 86, 197
Hin1II CATG 3 cut(s) 84, 377, 548
Hin6I GCGC 2 cut(s) 84, 195
HinP1I GCGC 2 cut(s) 84, 195
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HindIII AAGCTT 1 cut(s) 350
HinfI GANTC 4 cut(s) 455, 575, 587, 593
HpaII CCGG 3 cut(s) 114, 491, 510
Hpy166II GTNNAC 3 cut(s) 181, 334, 459
Hpy188I TCNGA 2 cut(s) 574, 592
Hpy188III TCNNGA 1 cut(s) 151
Hpy8I GTNNAC 3 cut(s) 181, 334, 459
HpyAV CCTTC 2 cut(s) 250, 388
HpyCH4III ACNGT 4 cut(s) 121, 251, 430, 517
HpyCH4V TGCA 3 cut(s) 171, 373, 401
HpyF10VI GCNNNNNNNGC 1 cut(s) 225
HpyF3I CTNAG 2 cut(s) 191, 353
Hsp92II CATG 3 cut(s) 84, 377, 548
HspAI GCGC 2 cut(s) 84, 195
KpnI GGTACC 1 cut(s) 559
LguI GCTCTTC 1 cut(s) 25
LmnI GCTCC 1 cut(s) 108
LweI GCATC 2 cut(s) 69, 409
MaeI CTAG 1 cut(s) 15
MaeIII GTNAC 3 cut(s) 251, 289, 408
MboII GAAGA 6 cut(s) 12, 115, 228, 478, 555, 570
MfeI CAATTG 1 cut(s) 504
MluCI AATT 2 cut(s) 504, 549
MlyI GAGTC 2 cut(s) 464, 602
MmeI TCCRAC 1 cut(s) 348
MnlI CCTC 6 cut(s) 34, 104, 116, 149, 322, 498
MseI TTAA 1 cut(s) 77
MslI CAYNNNNRTG 1 cut(s) 79
MspA1I CMGCKG 1 cut(s) 176
MspI CCGG 3 cut(s) 114, 491, 510
MspR9I CCNGG 1 cut(s) 447
MunI CAATTG 1 cut(s) 504
MvaI CCWGG 1 cut(s) 447
MwoI GCNNNNNNNGC 1 cut(s) 225
NcoI CCATGG 1 cut(s) 544
NlaIII CATG 3 cut(s) 84, 377, 548
NlaIV GGNNCC 1 cut(s) 557
NmuCI GTSAC 1 cut(s) 408
NsbI TGCGCA 1 cut(s) 85
NspI RCATGY 2 cut(s) 84, 377
PaeI GCATGC 1 cut(s) 377
PciSI GCTCTTC 1 cut(s) 25
PfeI GAWTC 2 cut(s) 575, 587
PkrI GCNGC 2 cut(s) 218, 601
PleI GAGTC 2 cut(s) 463, 601
PpsI GAGTC 2 cut(s) 463, 601
Psp6I CCWGG 1 cut(s) 445
PspGI CCWGG 1 cut(s) 445
PspN4I GGNNCC 1 cut(s) 557
PspPI GGNCC 1 cut(s) 309
RsaI GTAC 2 cut(s) 502, 557
RsaNI GTAC 2 cut(s) 501, 556
RseI CAYNNNNRTG 1 cut(s) 79
SapI GCTCTTC 1 cut(s) 25
SaqAI TTAA 1 cut(s) 77
SatI GCNGC 2 cut(s) 217, 600
Sau96I GGNCC 1 cut(s) 309
SchI GAGTC 2 cut(s) 464, 602
ScrFI CCNGG 1 cut(s) 447
SetI ASST 9 cut(s) 20, 96, 105, 354, 359, 437, 483, 502, 604
SfaNI GCATC 2 cut(s) 69, 409
SfcI CTRYAG 1 cut(s) 476
SmiMI CAYNNNNRTG 1 cut(s) 79
SphI GCATGC 1 cut(s) 377
Sse9I AATT 2 cut(s) 504, 549
SsiI CCGC 2 cut(s) 174, 216
SspMI CTAG 1 cut(s) 15
StyD4I CCNGG 1 cut(s) 445
StyI CCWWGG 1 cut(s) 544
TaaI ACNGT 4 cut(s) 121, 251, 430, 517
TaqI TCGA 1 cut(s) 314
TasI AATT 2 cut(s) 504, 549
TauI GCSGC 1 cut(s) 219
TfiI GAWTC 2 cut(s) 575, 587
Tru1I TTAA 1 cut(s) 77
Tru9I TTAA 1 cut(s) 77
TscAI CASTG 2 cut(s) 268, 433
TseFI GTSAC 1 cut(s) 408
TseI GCWGC 1 cut(s) 599
Tsp45I GTSAC 1 cut(s) 408
TspRI CASTG 2 cut(s) 268, 433
XceI RCATGY 2 cut(s) 84, 377
XspI CTAG 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.