Rh4DG222400

Protein of unknown function (DUF679)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0003054
Physical Location & Seq
Reverse (-)
42390654 .. 42452638
61985 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG222400.1

Sequence Viewer

Length: 609 bp
ATGGGGGCTTCATCCAGCGCTTCATCCAAATCCAAGAAATCCACCAAAAGCAAGGGTGTCAAAGACATTGCATTAACAGGTGTAGGCAACCTCATCAAGCTCCTCCCTACCGGCACGGTATTCTTGTTCCAGTTCGTCAACCCCGTTTTAACCAACAATGGCGAATGCAACATGTCCGTCAACAAGATTCTAAGCGCCATTCTCATTGTCGTTTGCGCCTTCTCTTGCTGCTTTGCTTCCTTCACCGACAGTTACACCTGCAGTGATGGACAAATTCACTACGGAATTGCAACAACAAAGGGTCTTTGGCCCTCAACCAGTTCTGGCTCTGTGGACTTGTCTGCTTACAAGCTTCGGGTTGGGGATTTTGTGCATGCCTTCTTTTCCTTGATCGTGTTTGCAGGGTTATCACTTTTGGATTCCAACACTGTGAGGTGCTTCTATGCTGCATTTGAGTCCACTGAGAAGGTTCTGCTCCAGGCGTTGCCCCCTGTAATAGGCACTATTGCTGGTACTATCTTTGTTCTGTTCCCTAATACACGCCACGGAATTGGGTACCCTTATTCAAGTTCTGATTCTTCACAAGACTCGGACCCGGAGGACTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF679

Protein Analysis

202

Amino Acids

21.42

Weight (kDa)

6.8

Isoelectric Point (pI)

38.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF679 PF05078 27 - 186 6e-62 Protein of unknown function (DUF679)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 266
Acc36I ACCTGC 1 cut(s) 266
Acc65I GGTACC 1 cut(s) 555
AccB1I GGYRCC 1 cut(s) 555
AcsI RAATTY 1 cut(s) 273
AfaI GTAC 2 cut(s) 514, 557
AfeI AGCGCT 1 cut(s) 19
AfiI CCNNNNNNNGG 1 cut(s) 497
AflIII ACRYGT 1 cut(s) 171
AgsI TTSAA 1 cut(s) 567
AjnI CCWGG 1 cut(s) 477
AluBI AGCT 2 cut(s) 100, 352
AluI AGCT 2 cut(s) 100, 352
Aor51HI AGCGCT 1 cut(s) 19
AoxI GGCC 1 cut(s) 308
ApeKI GCWGC 2 cut(s) 228, 446
ApoI RAATTY 1 cut(s) 273
Asp718I GGTACC 1 cut(s) 555
AspLEI GCGC 3 cut(s) 20, 197, 218
AspS9I GGNCC 2 cut(s) 309, 592
AsuC2I CCSGG 1 cut(s) 596
AsuHPI GGTGA 1 cut(s) 235
AvaII GGWCC 1 cut(s) 592
BanI GGYRCC 1 cut(s) 555
BbvI GCAGC 2 cut(s) 215, 433
BccI CCATC 1 cut(s) 260
BciT130I CCWGG 1 cut(s) 479
BcnI CCSGG 1 cut(s) 596
BfmI CTRYAG 1 cut(s) 259
BfoI RGCGCY 2 cut(s) 21, 198
BfuAI ACCTGC 1 cut(s) 266
BisI GCNGC 2 cut(s) 229, 447
BlsI GCNGC 2 cut(s) 230, 448
Bme1390I CCNGG 2 cut(s) 479, 596
Bme18I GGWCC 1 cut(s) 592
BmgT120I GGNCC 2 cut(s) 309, 592
BmiI GGNNCC 2 cut(s) 557, 594
BmrFI CCNGG 2 cut(s) 479, 596
BpmI CTGGAG 1 cut(s) 461
BpuMI CCSGG 1 cut(s) 596
BsaJI CCNNGG 1 cut(s) 544
Bsc4I CCNNNNNNNGG 1 cut(s) 497
Bse118I RCCGGY 1 cut(s) 110
Bse1I ACTGG 2 cut(s) 130, 318
Bse3DI GCAATG 1 cut(s) 66
BseBI CCWGG 1 cut(s) 479
BseDI CCNNGG 1 cut(s) 544
BseGI GGATG 2 cut(s) 11, 23
BseLI CCNNNNNNNGG 1 cut(s) 497
BseMI GCAATG 1 cut(s) 66
BseMII CTCAG 1 cut(s) 453
BseNI ACTGG 2 cut(s) 130, 318
BseRI GAGGAG 1 cut(s) 92
BseXI GCAGC 2 cut(s) 215, 433
BshFI GGCC 1 cut(s) 310
BshNI GGYRCC 1 cut(s) 555
BsiSI CCGG 2 cut(s) 111, 596
BslI CCNNNNNNNGG 1 cut(s) 497
BsmI GAATGC 1 cut(s) 170
BsnI GGCC 1 cut(s) 310
Bsp143I GATC 1 cut(s) 390
BspANI GGCC 1 cut(s) 310
BspCNI CTCAG 1 cut(s) 454
BspLI GGNNCC 2 cut(s) 557, 594
BspMAI CTGCAG 1 cut(s) 263
BspMI ACCTGC 1 cut(s) 266
BspT107I GGYRCC 1 cut(s) 555
BsrDI GCAATG 1 cut(s) 66
BsrFI RCCGGY 1 cut(s) 110
BsrI ACTGG 2 cut(s) 130, 318
BssAI RCCGGY 1 cut(s) 110
BssECI CCNNGG 1 cut(s) 544
BssMI GATC 1 cut(s) 390
Bst2UI CCWGG 1 cut(s) 479
Bst4CI ACNGT 3 cut(s) 118, 251, 430
BstC8I GCNNGC 1 cut(s) 375
BstDEI CTNAG 2 cut(s) 191, 462
BstDSI CCRYGG 1 cut(s) 544
BstENI CCTNNNNNAGG 1 cut(s) 495
BstF5I GGATG 2 cut(s) 11, 23
BstH2I RGCGCY 2 cut(s) 21, 198
BstHHI GCGC 3 cut(s) 20, 197, 218
BstKTI GATC 1 cut(s) 393
BstMBI GATC 1 cut(s) 390
BstNI CCWGG 1 cut(s) 479
BstNSI RCATGY 2 cut(s) 175, 377
BstSCI CCNGG 2 cut(s) 477, 594
BstSFI CTRYAG 1 cut(s) 259
BstV1I GCAGC 2 cut(s) 215, 433
BstXI CCANNNNNNTGG 1 cut(s) 551
BsuRI GGCC 1 cut(s) 310
BtgI CCRYGG 1 cut(s) 544
BtsCI GGATG 2 cut(s) 11, 23
BtsI GCAGTG 1 cut(s) 268
BtsIMutI CAGTG 3 cut(s) 268, 426, 459
BveI ACCTGC 1 cut(s) 266
Cac8I GCNNGC 1 cut(s) 375
CfoI GCGC 3 cut(s) 20, 197, 218
Cfr10I RCCGGY 1 cut(s) 110
Cfr13I GGNCC 2 cut(s) 309, 592
Csp6I GTAC 2 cut(s) 513, 556
CviAII CATG 2 cut(s) 172, 374
CviJI RGCY 5 cut(s) 8, 100, 310, 327, 352
CviKI_1 RGCY 5 cut(s) 8, 100, 310, 327, 352
CviQI GTAC 2 cut(s) 513, 556
DdeI CTNAG 2 cut(s) 191, 462
DpnI GATC 1 cut(s) 392
DpnII GATC 1 cut(s) 390
Eco47I GGWCC 1 cut(s) 592
Eco47III AGCGCT 1 cut(s) 19
EcoNI CCTNNNNNAGG 1 cut(s) 495
EcoRII CCWGG 1 cut(s) 477
FaeI CATG 2 cut(s) 175, 377
FaiI YATR 3 cut(s) 173, 375, 444
FatI CATG 2 cut(s) 171, 373
Fnu4HI GCNGC 2 cut(s) 229, 447
FokI GGATG 1 cut(s) 10
Fsp4HI GCNGC 2 cut(s) 229, 447
GlaI GCGC 3 cut(s) 19, 196, 217
GluI GCNGC 2 cut(s) 229, 447
GsuI CTGGAG 1 cut(s) 461
HaeII RGCGCY 2 cut(s) 21, 198
HaeIII GGCC 1 cut(s) 310
HapII CCGG 2 cut(s) 111, 596
HhaI GCGC 3 cut(s) 20, 197, 218
Hin1II CATG 2 cut(s) 175, 377
Hin6I GCGC 3 cut(s) 18, 195, 216
HinP1I GCGC 3 cut(s) 18, 195, 216
HincII GTYRAC 2 cut(s) 139, 181
HindII GTYRAC 2 cut(s) 139, 181
HindIII AAGCTT 1 cut(s) 350
HinfI GANTC 6 cut(s) 187, 419, 455, 575, 587, 602
HpaII CCGG 2 cut(s) 111, 596
HphI GGTGA 1 cut(s) 235
Hpy166II GTNNAC 4 cut(s) 139, 181, 334, 459
Hpy188I TCNGA 2 cut(s) 574, 592
Hpy8I GTNNAC 4 cut(s) 139, 181, 334, 459
HpyAV CCTTC 4 cut(s) 229, 250, 388, 460
HpyCH4III ACNGT 3 cut(s) 118, 251, 430
HpyCH4V TGCA 7 cut(s) 71, 168, 261, 290, 373, 401, 449
HpyF3I CTNAG 2 cut(s) 191, 462
Hsp92II CATG 2 cut(s) 175, 377
HspAI GCGC 3 cut(s) 18, 195, 216
KpnI GGTACC 1 cut(s) 559
Kzo9I GATC 1 cut(s) 390
LmnI GCTCC 2 cut(s) 105, 480
Lsp1109I GCAGC 2 cut(s) 215, 433
MaeIII GTNAC 1 cut(s) 251
MalI GATC 1 cut(s) 392
MboI GATC 1 cut(s) 390
MboII GAAGA 1 cut(s) 570
MluCI AATT 3 cut(s) 273, 285, 549
MlyI GAGTC 3 cut(s) 464, 581, 596
MmeI TCCRAC 1 cut(s) 447
MnlI CCTC 5 cut(s) 101, 113, 322, 426, 592
MseI TTAA 2 cut(s) 74, 149
MspI CCGG 2 cut(s) 111, 596
MspR9I CCNGG 2 cut(s) 479, 596
Mva1269I GAATGC 1 cut(s) 170
MvaI CCWGG 1 cut(s) 479
NciI CCSGG 1 cut(s) 596
NdeII GATC 1 cut(s) 390
NlaIII CATG 2 cut(s) 175, 377
NlaIV GGNNCC 2 cut(s) 557, 594
NspI RCATGY 2 cut(s) 175, 377
PaeI GCATGC 1 cut(s) 377
PaqCI CACCTGC 1 cut(s) 266
PciI ACATGT 1 cut(s) 171
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PctI GAATGC 1 cut(s) 170
PfeI GAWTC 3 cut(s) 187, 419, 575
PkrI GCNGC 2 cut(s) 230, 448
PleI GAGTC 3 cut(s) 463, 581, 596
PpsI GAGTC 3 cut(s) 463, 581, 596
PscI ACATGT 1 cut(s) 171
Psp6I CCWGG 1 cut(s) 477
PspGI CCWGG 1 cut(s) 477
PspN4I GGNNCC 2 cut(s) 557, 594
PspPI GGNCC 2 cut(s) 309, 592
PstI CTGCAG 1 cut(s) 263
RsaI GTAC 2 cut(s) 514, 557
RsaNI GTAC 2 cut(s) 513, 556
SaqAI TTAA 2 cut(s) 74, 149
SatI GCNGC 2 cut(s) 229, 447
Sau3AI GATC 1 cut(s) 390
Sau96I GGNCC 2 cut(s) 309, 592
SchI GAGTC 3 cut(s) 464, 581, 596
ScrFI CCNGG 2 cut(s) 479, 596
SetI ASST 7 cut(s) 82, 93, 102, 260, 354, 437, 471
SfcI CTRYAG 1 cut(s) 259
SinI GGWCC 1 cut(s) 592
SphI GCATGC 1 cut(s) 377
Sse9I AATT 3 cut(s) 273, 285, 549
StyD4I CCNGG 2 cut(s) 477, 594
TaaI ACNGT 3 cut(s) 118, 251, 430
TasI AATT 3 cut(s) 273, 285, 549
TfiI GAWTC 3 cut(s) 187, 419, 575
Tru1I TTAA 2 cut(s) 74, 149
Tru9I TTAA 2 cut(s) 74, 149
TscAI CASTG 3 cut(s) 268, 433, 466
TseI GCWGC 2 cut(s) 228, 446
TspDTI ATGAA 1 cut(s) 12
TspGWI ACGGA 3 cut(s) 166, 297, 561
TspRI CASTG 3 cut(s) 268, 433, 466
VpaK11BI GGWCC 1 cut(s) 592
XagI CCTNNNNNAGG 1 cut(s) 495
XapI RAATTY 1 cut(s) 273
XceI RCATGY 2 cut(s) 175, 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.