MD13G1177200.v1.1

Protein of unknown function (DUF679)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0003054
Physical Location & Seq
Forward (+)
14709795 .. 14710394
600 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1177200.v1.1.491

Sequence Viewer

Length: 600 bp
ATGGGGGGTTCTACTAGCTCTTCCTCTAAATCCAAGACAACCACCACCAAAAACAAAAGCGTTCACAACAGAACATTAACATACGCAGGCAACCTCATCAAGCTCCTCCCAACCGGTAGTCTTCTTGTTCCAGTTCCTCAACCCTGTGTTGACCAACAACGGCAACTGCACCGCTGTCAACAAATACCTAGGCGCCATTCTCATCGCTGTTTCCGGCTTCTCCTGCTGCTTTGCTTCCTTCACAGACAAGGCAGTGATGGACAAACACACTATGGGGTGGCAACAAGCAAGGGGCTTTGTCCCTCAACAAGTTCCAACTCTGTGGACTTGTCTGCTTACAAGCTTAGGTTTGGGGACTTTGTGCATGCCTTCTTTTCCTTGATTGTGTTTGCAGTTGTGTCACTTTTGGACGCCAACACTGTCAGGTACTATTATCCAGGGTTTGAGTCCACTGAGAAGATTTTGCTACAGGAGTTGCCTCAGGTTATCGGTACAATTGCTGGAACTGTTTTCGCTGTGTTCCCTAATAAACGCCATGGAATTGGGTACCCTTCGTCTAGTCCTGATTCTTCACAGGATTCGGCGTCGGCAGCTTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF679

Protein Analysis

200

Amino Acids

21.72

Weight (kDa)

9.64

Isoelectric Point (pI)

50.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF679 PF05078 29 - 183 7.2e-35 Protein of unknown function (DUF679)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 546
AccB1I GGYRCC 2 cut(s) 192, 546
AciI CCGC 1 cut(s) 172
AcyI GRCGYC 3 cut(s) 193, 411, 584
AfaI GTAC 3 cut(s) 428, 493, 548
AgeI ACCGGT 1 cut(s) 113
AjnI CCWGG 1 cut(s) 436
AluBI AGCT 4 cut(s) 18, 103, 343, 593
AluI AGCT 4 cut(s) 18, 103, 343, 593
ApeKI GCWGC 2 cut(s) 226, 590
AsiGI ACCGGT 1 cut(s) 113
Asp718I GGTACC 1 cut(s) 546
AspA2I CCTAGG 1 cut(s) 188
AspLEI GCGC 1 cut(s) 195
AvrII CCTAGG 1 cut(s) 188
AxyI CCTNAGG 1 cut(s) 480
BanI GGYRCC 2 cut(s) 192, 546
BbsI GAAGAC 1 cut(s) 113
BbvI GCAGC 1 cut(s) 213
BccI CCATC 1 cut(s) 251
BceAI ACGGC 1 cut(s) 176
BciT130I CCWGG 1 cut(s) 438
BfaI CTAG 3 cut(s) 15, 189, 558
BfmI CTRYAG 1 cut(s) 467
BfoI RGCGCY 1 cut(s) 196
BisI GCNGC 2 cut(s) 227, 591
BlnI CCTAGG 1 cut(s) 188
BlsI GCNGC 2 cut(s) 228, 592
Bme1390I CCNGG 1 cut(s) 438
BmiI GGNNCC 2 cut(s) 194, 548
BmrFI CCNGG 1 cut(s) 438
BpiI GAAGAC 1 cut(s) 113
Bpu10I CCTNAGC 1 cut(s) 344
BsaHI GRCGYC 3 cut(s) 193, 411, 584
BsaJI CCNNGG 3 cut(s) 188, 437, 535
BsaWI WCCGGW 1 cut(s) 113
Bse118I RCCGGY 1 cut(s) 113
Bse1I ACTGG 1 cut(s) 131
Bse21I CCTNAGG 1 cut(s) 480
BseBI CCWGG 1 cut(s) 438
BseDI CCNNGG 3 cut(s) 188, 437, 535
BseMII CTCAG 2 cut(s) 444, 494
BseNI ACTGG 1 cut(s) 131
BseRI GAGGAG 1 cut(s) 95
BseXI GCAGC 1 cut(s) 213
BsgI GTGCAG 1 cut(s) 152
BshNI GGYRCC 2 cut(s) 192, 546
BshTI ACCGGT 1 cut(s) 113
BsiSI CCGG 2 cut(s) 114, 214
BslFI GGGAC 2 cut(s) 285, 368
BsmFI GGGAC 2 cut(s) 285, 368
Bsp19I CCATGG 1 cut(s) 535
BspACI CCGC 1 cut(s) 172
BspCNI CTCAG 2 cut(s) 445, 493
BspLI GGNNCC 2 cut(s) 194, 548
BspQI GCTCTTC 1 cut(s) 25
BspT107I GGYRCC 2 cut(s) 192, 546
BsrFI RCCGGY 1 cut(s) 113
BsrI ACTGG 1 cut(s) 131
BssAI RCCGGY 1 cut(s) 113
BssECI CCNNGG 3 cut(s) 188, 437, 535
BssNI GRCGYC 3 cut(s) 193, 411, 584
BssT1I CCWWGG 2 cut(s) 188, 535
Bst2UI CCWGG 1 cut(s) 438
Bst4CI ACNGT 2 cut(s) 421, 508
Bst6I CTCTTC 1 cut(s) 25
BstACI GRCGYC 3 cut(s) 193, 411, 584
BstC8I GCNNGC 2 cut(s) 88, 366
BstDEI CTNAG 3 cut(s) 344, 453, 480
BstDSI CCRYGG 1 cut(s) 535
BstH2I RGCGCY 1 cut(s) 196
BstHHI GCGC 1 cut(s) 195
BstMWI GCNNNNNNNGC 2 cut(s) 223, 590
BstNI CCWGG 1 cut(s) 438
BstNSI RCATGY 1 cut(s) 368
BstSCI CCNGG 1 cut(s) 436
BstSFI CTRYAG 1 cut(s) 467
BstV1I GCAGC 1 cut(s) 213
BstV2I GAAGAC 1 cut(s) 113
BstXI CCANNNNNNTGG 2 cut(s) 322, 542
Bsu36I CCTNAGG 1 cut(s) 480
BtgI CCRYGG 1 cut(s) 535
BtgZI GCGATG 1 cut(s) 188
BtsI GCAGTG 1 cut(s) 259
BtsIMutI CAGTG 3 cut(s) 259, 417, 450
Cac8I GCNNGC 2 cut(s) 88, 366
CfoI GCGC 1 cut(s) 195
Cfr10I RCCGGY 1 cut(s) 113
CseI GACGC 2 cut(s) 419, 573
Csp6I GTAC 3 cut(s) 427, 492, 547
CspAI ACCGGT 1 cut(s) 113
CviAII CATG 2 cut(s) 365, 536
CviJI RGCY 6 cut(s) 18, 103, 217, 295, 343, 593
CviKI_1 RGCY 6 cut(s) 18, 103, 217, 295, 343, 593
CviQI GTAC 3 cut(s) 427, 492, 547
DdeI CTNAG 3 cut(s) 344, 453, 480
DinI GGCGCC 1 cut(s) 194
Eam1104I CTCTTC 1 cut(s) 25
EarI CTCTTC 1 cut(s) 25
Eco130I CCWWGG 2 cut(s) 188, 535
Eco81I CCTNAGG 1 cut(s) 480
EcoRII CCWGG 1 cut(s) 436
EcoT14I CCWWGG 2 cut(s) 188, 535
EgeI GGCGCC 1 cut(s) 194
EheI GGCGCC 1 cut(s) 194
ErhI CCWWGG 2 cut(s) 188, 535
FaeI CATG 2 cut(s) 368, 539
FaiI YATR 4 cut(s) 82, 273, 366, 537
FaqI GGGAC 2 cut(s) 285, 368
FatI CATG 2 cut(s) 364, 535
Fnu4HI GCNGC 2 cut(s) 227, 591
Fsp4HI GCNGC 2 cut(s) 227, 591
FspBI CTAG 3 cut(s) 15, 189, 558
GlaI GCGC 1 cut(s) 194
GluI GCNGC 2 cut(s) 227, 591
HaeII RGCGCY 1 cut(s) 196
HapII CCGG 2 cut(s) 114, 214
HgaI GACGC 2 cut(s) 419, 573
HhaI GCGC 1 cut(s) 195
Hin1I GRCGYC 3 cut(s) 193, 411, 584
Hin1II CATG 2 cut(s) 368, 539
Hin6I GCGC 1 cut(s) 193
HinP1I GCGC 1 cut(s) 193
HincII GTYRAC 2 cut(s) 151, 179
HindII GTYRAC 2 cut(s) 151, 179
HindIII AAGCTT 1 cut(s) 341
HinfI GANTC 3 cut(s) 446, 566, 578
HpaII CCGG 2 cut(s) 114, 214
Hpy166II GTNNAC 5 cut(s) 64, 151, 179, 325, 450
Hpy188III TCNNGA 1 cut(s) 563
Hpy8I GTNNAC 5 cut(s) 64, 151, 179, 325, 450
Hpy99I CGWCG 1 cut(s) 589
HpyAV CCTTC 3 cut(s) 248, 379, 561
HpyCH4III ACNGT 2 cut(s) 421, 508
HpyCH4V TGCA 3 cut(s) 169, 364, 392
HpyF10VI GCNNNNNNNGC 2 cut(s) 223, 590
HpyF3I CTNAG 3 cut(s) 344, 453, 480
Hsp92I GRCGYC 3 cut(s) 193, 411, 584
Hsp92II CATG 2 cut(s) 368, 539
HspAI GCGC 1 cut(s) 193
KasI GGCGCC 1 cut(s) 192
KpnI GGTACC 1 cut(s) 550
LguI GCTCTTC 1 cut(s) 25
LmnI GCTCC 1 cut(s) 108
Lsp1109I GCAGC 1 cut(s) 213
MaeI CTAG 3 cut(s) 15, 189, 558
MaeIII GTNAC 1 cut(s) 399
MboII GAAGA 4 cut(s) 12, 113, 469, 561
MfeI CAATTG 1 cut(s) 495
MluCI AATT 2 cut(s) 495, 540
Mly113I GGCGCC 1 cut(s) 193
MlyI GAGTC 1 cut(s) 455
MmeI TCCRAC 1 cut(s) 339
MnlI CCTC 6 cut(s) 34, 104, 116, 147, 313, 489
MseI TTAA 1 cut(s) 77
MspA1I CMGCKG 1 cut(s) 174
MspI CCGG 2 cut(s) 114, 214
MspR9I CCNGG 1 cut(s) 438
MunI CAATTG 1 cut(s) 495
MvaI CCWGG 1 cut(s) 438
MwoI GCNNNNNNNGC 2 cut(s) 223, 590
NarI GGCGCC 1 cut(s) 193
NcoI CCATGG 1 cut(s) 535
NlaIII CATG 2 cut(s) 368, 539
NlaIV GGNNCC 2 cut(s) 194, 548
NmuCI GTSAC 1 cut(s) 399
NspI RCATGY 1 cut(s) 368
PaeI GCATGC 1 cut(s) 368
PciSI GCTCTTC 1 cut(s) 25
PfeI GAWTC 2 cut(s) 566, 578
PinAI ACCGGT 1 cut(s) 113
PkrI GCNGC 2 cut(s) 228, 592
PleI GAGTC 1 cut(s) 454
PluTI GGCGCC 1 cut(s) 196
PpsI GAGTC 1 cut(s) 454
Psp6I CCWGG 1 cut(s) 436
PspGI CCWGG 1 cut(s) 436
PspN4I GGNNCC 2 cut(s) 194, 548
RsaI GTAC 3 cut(s) 428, 493, 548
RsaNI GTAC 3 cut(s) 427, 492, 547
SapI GCTCTTC 1 cut(s) 25
SaqAI TTAA 1 cut(s) 77
SatI GCNGC 2 cut(s) 227, 591
SchI GAGTC 1 cut(s) 455
ScrFI CCNGG 1 cut(s) 438
SetI ASST 9 cut(s) 20, 96, 105, 190, 345, 350, 428, 486, 595
SfcI CTRYAG 1 cut(s) 467
SfoI GGCGCC 1 cut(s) 194
SphI GCATGC 1 cut(s) 368
Sse9I AATT 2 cut(s) 495, 540
SsiI CCGC 1 cut(s) 172
SspDI GGCGCC 1 cut(s) 192
SspMI CTAG 3 cut(s) 15, 189, 558
StyD4I CCNGG 1 cut(s) 436
StyI CCWWGG 2 cut(s) 188, 535
TaaI ACNGT 2 cut(s) 421, 508
TasI AATT 2 cut(s) 495, 540
TfiI GAWTC 2 cut(s) 566, 578
Tru1I TTAA 1 cut(s) 77
Tru9I TTAA 1 cut(s) 77
TscAI CASTG 3 cut(s) 259, 424, 457
TseFI GTSAC 1 cut(s) 399
TseI GCWGC 2 cut(s) 226, 590
Tsp45I GTSAC 1 cut(s) 399
TspRI CASTG 3 cut(s) 259, 424, 457
XceI RCATGY 1 cut(s) 368
XmaJI CCTAGG 1 cut(s) 188
XspI CTAG 3 cut(s) 15, 189, 558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.