FvH4_4g16720

Protein of unknown function (DUF679)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0003054
Physical Location & Seq
Reverse (-)
20564787 .. 20565392
606 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g16720.t1

Sequence Viewer

Length: 606 bp
ATGGGGGCTTCATCTAGTGCTTCTTCCTCCAAATCAAAGAAATCCACCAAAAACAAGGGTGTCAAAGACATTGCATTAACAGGAGTAGGCAACCTCATTAAGCTCCTCCCTACCGGCACGGTCTTCTTGTTCCAGTTCCTCAACCCCGTCTTAACCAACAATGGCCACTGCAACAACCCCGTCAACAAGTATCTAAGCGCCATTCTCATTGCCATTTCAGCCTTCTCTTGCTGCTTTTCTTCCTTCACTGACAGTTACATCGGCAGTGATGGACAAACTCACTATGGAATTGCAACAACAAAGGGTCTTTGGCCCTCAACCAGTTCTGGCTCTATGGACTTGTCTGCTTACAAGCTTCGGGTTGGGGACTTTGTGCATGCCTTCCTTTCCTTGACCGTGTTTGCAGTGGTATCACTTTTGGATTCCAACACTGTGAGGTGCTTCTATCCTACATTTGAGTCCACTGAGAGGGTTTTGCTCATGGTGTTGCCTCCTGTAATTGGCACTATTGCTGGTACTGTCTTTGTTGTGTTCCCTAATAAACGCCACGGAATTGGCTACCCTTCTTCAAGTTCAGATTCTTCACAAGACTCGGAGGACTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF679

Protein Analysis

202

Amino Acids

21.37

Weight (kDa)

8.67

Isoelectric Point (pI)

43.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF679 PF05078 28 - 187 6.2e-64 Protein of unknown function (DUF679)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 163
AfaI GTAC 1 cut(s) 517
AfiI CCNNNNNNNGG 2 cut(s) 468, 500
AgsI TTSAA 1 cut(s) 570
AluBI AGCT 2 cut(s) 103, 355
AluI AGCT 2 cut(s) 103, 355
AoxI GGCC 2 cut(s) 163, 311
ApeKI GCWGC 1 cut(s) 231
AspLEI GCGC 1 cut(s) 200
AspS9I GGNCC 1 cut(s) 312
BalI TGGCCA 1 cut(s) 165
BbsI GAAGAC 1 cut(s) 115
BbvI GCAGC 1 cut(s) 218
BccI CCATC 1 cut(s) 263
BfaI CTAG 2 cut(s) 15, 604
BfoI RGCGCY 1 cut(s) 201
BisI GCNGC 1 cut(s) 232
BlsI GCNGC 1 cut(s) 233
BmgT120I GGNCC 1 cut(s) 312
BpiI GAAGAC 1 cut(s) 115
BsaJI CCNNGG 1 cut(s) 547
Bsc4I CCNNNNNNNGG 2 cut(s) 468, 500
Bse118I RCCGGY 1 cut(s) 113
Bse1I ACTGG 2 cut(s) 133, 321
Bse3DI GCAATG 2 cut(s) 69, 207
BseDI CCNNGG 1 cut(s) 547
BseLI CCNNNNNNNGG 2 cut(s) 468, 500
BseMI GCAATG 2 cut(s) 69, 207
BseMII CTCAG 1 cut(s) 456
BseNI ACTGG 2 cut(s) 133, 321
BseRI GAGGAG 1 cut(s) 95
BseXI GCAGC 1 cut(s) 218
BshFI GGCC 2 cut(s) 165, 313
BsiSI CCGG 1 cut(s) 114
BslFI GGGAC 1 cut(s) 380
BslI CCNNNNNNNGG 2 cut(s) 468, 500
BsmFI GGGAC 1 cut(s) 380
BsnI GGCC 2 cut(s) 165, 313
BspANI GGCC 2 cut(s) 165, 313
BspCNI CTCAG 1 cut(s) 457
BsrDI GCAATG 2 cut(s) 69, 207
BsrFI RCCGGY 1 cut(s) 113
BsrI ACTGG 2 cut(s) 133, 321
BssAI RCCGGY 1 cut(s) 113
BssECI CCNNGG 1 cut(s) 547
Bst4CI ACNGT 5 cut(s) 121, 254, 397, 433, 520
BstC8I GCNNGC 1 cut(s) 378
BstDEI CTNAG 2 cut(s) 194, 465
BstDSI CCRYGG 1 cut(s) 547
BstH2I RGCGCY 1 cut(s) 201
BstHHI GCGC 1 cut(s) 200
BstMWI GCNNNNNNNGC 1 cut(s) 218
BstNSI RCATGY 1 cut(s) 380
BstV1I GCAGC 1 cut(s) 218
BstV2I GAAGAC 1 cut(s) 115
BstXI CCANNNNNNTGG 1 cut(s) 554
BsuRI GGCC 2 cut(s) 165, 313
BtgI CCRYGG 1 cut(s) 547
BtsI GCAGTG 3 cut(s) 166, 271, 411
BtsIMutI CAGTG 6 cut(s) 166, 246, 271, 411, 429, 462
Cac8I GCNNGC 1 cut(s) 378
CfoI GCGC 1 cut(s) 200
Cfr10I RCCGGY 1 cut(s) 113
Cfr13I GGNCC 1 cut(s) 312
Csp6I GTAC 1 cut(s) 516
CviAII CATG 2 cut(s) 377, 481
CviJI RGCY 8 cut(s) 8, 103, 165, 221, 313, 330, 355, 558
CviKI_1 RGCY 8 cut(s) 8, 103, 165, 221, 313, 330, 355, 558
CviQI GTAC 1 cut(s) 516
DdeI CTNAG 2 cut(s) 194, 465
EaeI YGGCCR 1 cut(s) 163
FaeI CATG 2 cut(s) 380, 484
FaiI YATR 4 cut(s) 285, 335, 378, 482
FaqI GGGAC 1 cut(s) 380
FatI CATG 2 cut(s) 376, 480
Fnu4HI GCNGC 1 cut(s) 232
Fsp4HI GCNGC 1 cut(s) 232
FspBI CTAG 2 cut(s) 15, 604
GlaI GCGC 1 cut(s) 199
GluI GCNGC 1 cut(s) 232
HaeII RGCGCY 1 cut(s) 201
HaeIII GGCC 2 cut(s) 165, 313
HapII CCGG 1 cut(s) 114
HhaI GCGC 1 cut(s) 200
Hin1II CATG 2 cut(s) 380, 484
Hin6I GCGC 1 cut(s) 198
HinP1I GCGC 1 cut(s) 198
HincII GTYRAC 1 cut(s) 184
HindII GTYRAC 1 cut(s) 184
HindIII AAGCTT 1 cut(s) 353
HinfI GANTC 5 cut(s) 422, 458, 578, 590, 599
HpaII CCGG 1 cut(s) 114
Hpy166II GTNNAC 2 cut(s) 184, 462
Hpy188I TCNGA 2 cut(s) 577, 595
Hpy8I GTNNAC 2 cut(s) 184, 462
HpyAV CCTTC 4 cut(s) 232, 253, 391, 573
HpyCH4III ACNGT 5 cut(s) 121, 254, 397, 433, 520
HpyCH4V TGCA 5 cut(s) 74, 171, 293, 376, 404
HpyF10VI GCNNNNNNNGC 1 cut(s) 218
HpyF3I CTNAG 2 cut(s) 194, 465
Hsp92II CATG 2 cut(s) 380, 484
HspAI GCGC 1 cut(s) 198
LmnI GCTCC 1 cut(s) 108
LpnPI CCDG 7 cut(s) 66, 127, 146, 312, 334, 498, 507
Lsp1109I GCAGC 1 cut(s) 218
MaeI CTAG 2 cut(s) 15, 604
MaeIII GTNAC 1 cut(s) 254
MboII GAAGA 5 cut(s) 15, 115, 231, 558, 573
MlsI TGGCCA 1 cut(s) 165
MluCI AATT 3 cut(s) 288, 498, 552
MluNI TGGCCA 1 cut(s) 165
MlyI GAGTC 3 cut(s) 467, 584, 593
MmeI TCCRAC 1 cut(s) 450
MnlI CCTC 9 cut(s) 37, 104, 116, 149, 325, 429, 462, 501, 589
Mox20I TGGCCA 1 cut(s) 165
MscI TGGCCA 1 cut(s) 165
MseI TTAA 3 cut(s) 77, 99, 152
Msp20I TGGCCA 1 cut(s) 165
MspI CCGG 1 cut(s) 114
MwoI GCNNNNNNNGC 1 cut(s) 218
NlaIII CATG 2 cut(s) 380, 484
NspI RCATGY 1 cut(s) 380
PaeI GCATGC 1 cut(s) 380
PfeI GAWTC 2 cut(s) 422, 578
PkrI GCNGC 1 cut(s) 233
PleI GAGTC 3 cut(s) 466, 584, 593
PpsI GAGTC 3 cut(s) 466, 584, 593
PspPI GGNCC 1 cut(s) 312
RsaI GTAC 1 cut(s) 517
RsaNI GTAC 1 cut(s) 516
SaqAI TTAA 3 cut(s) 77, 99, 152
SatI GCNGC 1 cut(s) 232
Sau96I GGNCC 1 cut(s) 312
SchI GAGTC 3 cut(s) 467, 584, 593
SetI ASST 4 cut(s) 96, 105, 357, 440
SphI GCATGC 1 cut(s) 380
Sse9I AATT 3 cut(s) 288, 498, 552
SspMI CTAG 2 cut(s) 15, 604
TaaI ACNGT 5 cut(s) 121, 254, 397, 433, 520
TasI AATT 3 cut(s) 288, 498, 552
TfiI GAWTC 2 cut(s) 422, 578
Tru1I TTAA 3 cut(s) 77, 99, 152
Tru9I TTAA 3 cut(s) 77, 99, 152
TscAI CASTG 6 cut(s) 173, 253, 271, 411, 436, 469
TseI GCWGC 1 cut(s) 231
TspGWI ACGGA 1 cut(s) 564
TspRI CASTG 6 cut(s) 173, 253, 271, 411, 436, 469
XceI RCATGY 1 cut(s) 380
XspI CTAG 2 cut(s) 15, 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.