MD16G1178800.v1.1

Protein of unknown function (DUF679)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0003054
Physical Location & Seq
Forward (+)
15311733 .. 15312308
576 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1178800.v1.1.491

Sequence Viewer

Length: 576 bp
ATGGGGGGCTCTTCCTCTAAATCCAAGACAACCACCACCAAAAACAAAAGCGCCAAAGACAGAACATTCACAGGCGCAGGCAACCTCATCAAGCTCCTCCCGACCGGCACGGTCTTCTTGTTCCAGTTCCTCAACCCTGTCTTGACCAACAACGGCGACTGCACGGCCCTCAACAAATACCTAAGCGCCATTCTCATCGTCCTTTGCGGCTTCTCTTGCTGCTTTGCTTCCTTCACAGACAGTTACACTGGGAGTGATGGACAAACACACTATGGTGTGGCAACATGCAAGGGTCTTTGGCCCTCAACAAACTCCAATTCTGTAGACTTGTCTGCTTATAAGCTTAGGATTGGGGACTTTGTGCATGCCTTCTTTTCCGTGATTGTGTTTGCAGTTTTATCACTTTTGGATACCAACACTGTCAGGTGCTATTATCCAGGGTTTGAGTCCACTGAGAAGACTTTGCTGCAGGTGTTGCCTCCGGTTATTGGTACAATTGCCGGTACTGTTTTCGTTGTGTTCCCTAACAACCGCCATGGAATCGGGTACCCCGCGTCAAGTTCTGATTCTTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF679

Protein Analysis

192

Amino Acids

20.4

Weight (kDa)

8.74

Isoelectric Point (pI)

29.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF679 PF05078 25 - 183 4.6e-66 Protein of unknown function (DUF679)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 339
AarI CACCTGC 1 cut(s) 460
Acc36I ACCTGC 1 cut(s) 460
Acc65I GGTACC 1 cut(s) 546
AccB1I GGYRCC 1 cut(s) 546
AccI GTMKAC 1 cut(s) 324
AccII CGCG 1 cut(s) 554
AciI CCGC 3 cut(s) 207, 532, 552
AfaI GTAC 3 cut(s) 493, 505, 548
AfiI CCNNNNNNNGG 1 cut(s) 488
AjnI CCWGG 1 cut(s) 436
AleI CACNNNNGTG 1 cut(s) 273
AluBI AGCT 2 cut(s) 94, 343
AluI AGCT 2 cut(s) 94, 343
AoxI GGCC 2 cut(s) 165, 299
ApeKI GCWGC 2 cut(s) 219, 466
Asp718I GGTACC 1 cut(s) 546
AspLEI GCGC 3 cut(s) 53, 77, 188
AspS9I GGNCC 2 cut(s) 166, 300
BanI GGYRCC 1 cut(s) 546
BanII GRGCYC 1 cut(s) 11
BbsI GAAGAC 2 cut(s) 106, 464
BbvI GCAGC 2 cut(s) 206, 453
BccI CCATC 1 cut(s) 251
BceAI ACGGC 2 cut(s) 169, 180
BciT130I CCWGG 1 cut(s) 438
BciVI GTATCC 1 cut(s) 403
BfmI CTRYAG 2 cut(s) 321, 467
BfoI RGCGCY 2 cut(s) 54, 189
BfuAI ACCTGC 1 cut(s) 460
BfuI GTATCC 1 cut(s) 403
BisI GCNGC 3 cut(s) 208, 220, 467
BlsI GCNGC 3 cut(s) 209, 221, 468
Bme1390I CCNGG 1 cut(s) 438
BmgT120I GGNCC 2 cut(s) 166, 300
BmiI GGNNCC 1 cut(s) 548
BmrFI CCNGG 1 cut(s) 438
BmrI ACTGGG 1 cut(s) 258
BmuI ACTGGG 1 cut(s) 258
BpiI GAAGAC 2 cut(s) 106, 464
Bpu10I CCTNAGC 2 cut(s) 182, 344
BsaJI CCNNGG 2 cut(s) 437, 535
BsaWI WCCGGW 1 cut(s) 481
Bsc4I CCNNNNNNNGG 1 cut(s) 488
Bse118I RCCGGY 2 cut(s) 104, 500
Bse1I ACTGG 2 cut(s) 124, 253
BseBI CCWGG 1 cut(s) 438
BseDI CCNNGG 2 cut(s) 437, 535
BseLI CCNNNNNNNGG 1 cut(s) 488
BseMII CTCAG 1 cut(s) 444
BseNI ACTGG 2 cut(s) 124, 253
BseRI GAGGAG 1 cut(s) 86
BseXI GCAGC 2 cut(s) 206, 453
BsgI GTGCAG 1 cut(s) 145
Bsh1236I CGCG 1 cut(s) 554
Bsh1285I CGRYCG 1 cut(s) 105
BshFI GGCC 2 cut(s) 167, 301
BshNI GGYRCC 1 cut(s) 546
BsiEI CGRYCG 1 cut(s) 105
BsiSI CCGG 3 cut(s) 105, 482, 501
BslFI GGGAC 1 cut(s) 368
BslI CCNNNNNNNGG 1 cut(s) 488
BsmFI GGGAC 1 cut(s) 368
BsnI GGCC 2 cut(s) 167, 301
Bsp1286I GDGCHC 1 cut(s) 11
Bsp19I CCATGG 1 cut(s) 535
BspACI CCGC 3 cut(s) 207, 532, 552
BspANI GGCC 2 cut(s) 167, 301
BspCNI CTCAG 1 cut(s) 445
BspFNI CGCG 1 cut(s) 554
BspLI GGNNCC 1 cut(s) 548
BspMAI CTGCAG 1 cut(s) 471
BspMI ACCTGC 1 cut(s) 460
BspQI GCTCTTC 1 cut(s) 16
BspT107I GGYRCC 1 cut(s) 546
BsrFI RCCGGY 2 cut(s) 104, 500
BsrI ACTGG 2 cut(s) 124, 253
BssAI RCCGGY 2 cut(s) 104, 500
BssECI CCNNGG 2 cut(s) 437, 535
BssT1I CCWWGG 1 cut(s) 535
Bst2UI CCWGG 1 cut(s) 438
Bst4CI ACNGT 4 cut(s) 112, 242, 421, 508
Bst6I CTCTTC 1 cut(s) 16
BstAPI GCANNNNNTGC 1 cut(s) 475
BstC8I GCNNGC 2 cut(s) 79, 366
BstDEI CTNAG 3 cut(s) 182, 344, 453
BstDSI CCRYGG 1 cut(s) 535
BstFNI CGCG 1 cut(s) 554
BstH2I RGCGCY 2 cut(s) 54, 189
BstHHI GCGC 3 cut(s) 53, 77, 188
BstMCI CGRYCG 1 cut(s) 105
BstMWI GCNNNNNNNGC 2 cut(s) 216, 475
BstNI CCWGG 1 cut(s) 438
BstNSI RCATGY 2 cut(s) 288, 368
BstSCI CCNGG 1 cut(s) 436
BstSFI CTRYAG 2 cut(s) 321, 467
BstUI CGCG 1 cut(s) 554
BstV1I GCAGC 2 cut(s) 206, 453
BstV2I GAAGAC 2 cut(s) 106, 464
BsuI GTATCC 1 cut(s) 403
BsuRI GGCC 2 cut(s) 167, 301
BtgI CCRYGG 1 cut(s) 535
BtsIMutI CAGTG 3 cut(s) 246, 417, 450
BveI ACCTGC 1 cut(s) 460
Cac8I GCNNGC 2 cut(s) 79, 366
CfoI GCGC 3 cut(s) 53, 77, 188
Cfr10I RCCGGY 2 cut(s) 104, 500
Cfr13I GGNCC 2 cut(s) 166, 300
CseI GACGC 1 cut(s) 543
Csp6I GTAC 3 cut(s) 492, 504, 547
CviAII CATG 3 cut(s) 285, 365, 536
CviJI RGCY 6 cut(s) 9, 94, 167, 210, 301, 343
CviKI_1 RGCY 6 cut(s) 9, 94, 167, 210, 301, 343
CviQI GTAC 3 cut(s) 492, 504, 547
DdeI CTNAG 3 cut(s) 182, 344, 453
Eam1104I CTCTTC 1 cut(s) 16
EarI CTCTTC 1 cut(s) 16
Eco130I CCWWGG 1 cut(s) 535
Eco24I GRGCYC 1 cut(s) 11
EcoRII CCWGG 1 cut(s) 436
EcoT14I CCWWGG 1 cut(s) 535
EcoT38I GRGCYC 1 cut(s) 11
ErhI CCWWGG 1 cut(s) 535
FaeI CATG 3 cut(s) 288, 368, 539
FaiI YATR 5 cut(s) 273, 286, 339, 366, 537
FaqI GGGAC 1 cut(s) 368
FatI CATG 3 cut(s) 284, 364, 535
FauI CCCGC 1 cut(s) 559
FblI GTMKAC 1 cut(s) 324
Fnu4HI GCNGC 3 cut(s) 208, 220, 467
FriOI GRGCYC 1 cut(s) 11
Fsp4HI GCNGC 3 cut(s) 208, 220, 467
GlaI GCGC 3 cut(s) 52, 76, 187
GluI GCNGC 3 cut(s) 208, 220, 467
HaeII RGCGCY 2 cut(s) 54, 189
HaeIII GGCC 2 cut(s) 167, 301
HapII CCGG 3 cut(s) 105, 482, 501
HgaI GACGC 1 cut(s) 543
HhaI GCGC 3 cut(s) 53, 77, 188
Hin1II CATG 3 cut(s) 288, 368, 539
Hin6I GCGC 3 cut(s) 51, 75, 186
HinP1I GCGC 3 cut(s) 51, 75, 186
HindIII AAGCTT 1 cut(s) 341
HinfI GANTC 3 cut(s) 446, 540, 566
HpaII CCGG 3 cut(s) 105, 482, 501
Hpy166II GTNNAC 2 cut(s) 325, 450
Hpy188I TCNGA 1 cut(s) 565
Hpy188III TCNNGA 2 cut(s) 100, 142
Hpy8I GTNNAC 2 cut(s) 325, 450
HpyAV CCTTC 2 cut(s) 241, 379
HpyCH4III ACNGT 4 cut(s) 112, 242, 421, 508
HpyCH4V TGCA 5 cut(s) 162, 288, 364, 392, 469
HpyF10VI GCNNNNNNNGC 2 cut(s) 216, 475
HpyF3I CTNAG 3 cut(s) 182, 344, 453
Hsp92II CATG 3 cut(s) 288, 368, 539
HspAI GCGC 3 cut(s) 51, 75, 186
KpnI GGTACC 1 cut(s) 550
LguI GCTCTTC 1 cut(s) 16
LmnI GCTCC 1 cut(s) 99
Lsp1109I GCAGC 2 cut(s) 206, 453
MaeIII GTNAC 1 cut(s) 242
MboII GAAGA 4 cut(s) 3, 106, 469, 561
MfeI CAATTG 1 cut(s) 495
MhlI GDGCHC 1 cut(s) 11
MluCI AATT 2 cut(s) 316, 495
MlyI GAGTC 1 cut(s) 455
MnlI CCTC 7 cut(s) 25, 95, 107, 140, 179, 313, 489
MslI CAYNNNNRTG 1 cut(s) 273
MspI CCGG 3 cut(s) 105, 482, 501
MspR9I CCNGG 1 cut(s) 438
MunI CAATTG 1 cut(s) 495
MvaI CCWGG 1 cut(s) 438
MvnI CGCG 1 cut(s) 554
MwoI GCNNNNNNNGC 2 cut(s) 216, 475
NcoI CCATGG 1 cut(s) 535
NlaIII CATG 3 cut(s) 288, 368, 539
NlaIV GGNNCC 1 cut(s) 548
NspI RCATGY 2 cut(s) 288, 368
OliI CACNNNNGTG 1 cut(s) 273
PaeI GCATGC 1 cut(s) 368
PaqCI CACCTGC 1 cut(s) 460
PciSI GCTCTTC 1 cut(s) 16
PfeI GAWTC 2 cut(s) 540, 566
PkrI GCNGC 3 cut(s) 209, 221, 468
PleI GAGTC 1 cut(s) 454
PpsI GAGTC 1 cut(s) 454
PsiI TTATAA 1 cut(s) 339
Psp6I CCWGG 1 cut(s) 436
PspGI CCWGG 1 cut(s) 436
PspN4I GGNNCC 1 cut(s) 548
PspPI GGNCC 2 cut(s) 166, 300
PstI CTGCAG 1 cut(s) 471
RsaI GTAC 3 cut(s) 493, 505, 548
RsaNI GTAC 3 cut(s) 492, 504, 547
RseI CAYNNNNRTG 1 cut(s) 273
SapI GCTCTTC 1 cut(s) 16
SatI GCNGC 3 cut(s) 208, 220, 467
Sau96I GGNCC 2 cut(s) 166, 300
SchI GAGTC 1 cut(s) 455
ScrFI CCNGG 1 cut(s) 438
SduI GDGCHC 1 cut(s) 11
SetI ASST 6 cut(s) 87, 96, 183, 345, 428, 474
SfcI CTRYAG 2 cut(s) 321, 467
SmiMI CAYNNNNRTG 1 cut(s) 273
SphI GCATGC 1 cut(s) 368
Sse9I AATT 2 cut(s) 316, 495
SsiI CCGC 3 cut(s) 207, 532, 552
StyD4I CCNGG 1 cut(s) 436
StyI CCWWGG 1 cut(s) 535
TaaI ACNGT 4 cut(s) 112, 242, 421, 508
TasI AATT 2 cut(s) 316, 495
TauI GCSGC 1 cut(s) 210
TfiI GAWTC 2 cut(s) 540, 566
TscAI CASTG 3 cut(s) 253, 424, 457
TseI GCWGC 2 cut(s) 219, 466
TspGWI ACGGA 1 cut(s) 367
TspRI CASTG 3 cut(s) 253, 424, 457
XceI RCATGY 2 cut(s) 288, 368
XmiI GTMKAC 1 cut(s) 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.