MD15G1004000.v1.1

Protein of unknown function (DUF679)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003054
Physical Location & Seq
Reverse (-)
241967 .. 242548
582 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1004000.v1.1.491

Sequence Viewer

Length: 582 bp
ATGGGGGGTTCTACGAGCTCTTCCTATAAATCCAAGACAACCACCACAACCAACAAAAGCGTCAAAGACAAAACATTAACAGGCGCAGGCAACCTCATCAAGCTCCTCCCGACAGGCACGGTCTTCTTGTTCCAGTTCCTCAACCCTGTCTTGACCAACAACGGCAAATGCACTGCTGTCAACAAATACCTAAGCGCCGTTCTCATCGGTGTTTGCGGCTTCTCCTGCTGCTTTTCTTCCTTTACAGACAGTTACAAAGGCAGTGATGAACAAACACACTATGGGGTGGCAACATGCAAGGGGCTTTGGCCCTCGACGAGTTCAAACTCTGTGGATTTGTCTGCTTACAAGCTTAGGTTTGGGGACTTTGTGCATGCCTTCTTTTCCTTGATTGTGTTTGCAGTTGTGTCACTTTTGGACGCCAACACTGTCAGGTGCTATTATCCAGGGTTCGAGTCCACTGAGAAGGTTTTGCTACAGGTGTTGCCTCCGGTTATTGGTGCAATAGCCGGAACTGTTTTCGTTGTGTTCCCTAATAAACGCCATGGAATTGGGTACCCGTCGTCAAGTTCTGATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF679

Protein Analysis

194

Amino Acids

20.63

Weight (kDa)

9.01

Isoelectric Point (pI)

34.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF679 PF05078 28 - 186 4.8e-66 Protein of unknown function (DUF679)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 555
AccB1I GGYRCC 1 cut(s) 555
AciI CCGC 1 cut(s) 216
AcyI GRCGYC 1 cut(s) 420
AfaI GTAC 1 cut(s) 557
AfiI CCNNNNNNNGG 1 cut(s) 497
AgsI TTSAA 1 cut(s) 324
AjnI CCWGG 1 cut(s) 445
AluBI AGCT 3 cut(s) 18, 103, 352
AluI AGCT 3 cut(s) 18, 103, 352
Alw21I GWGCWC 1 cut(s) 20
AoxI GGCC 1 cut(s) 308
ApeKI GCWGC 1 cut(s) 228
Asp718I GGTACC 1 cut(s) 555
AspLEI GCGC 2 cut(s) 86, 197
AspS9I GGNCC 1 cut(s) 309
BanI GGYRCC 1 cut(s) 555
BanII GRGCYC 1 cut(s) 20
BbsI GAAGAC 1 cut(s) 115
Bbv12I GWGCWC 1 cut(s) 20
BbvI GCAGC 1 cut(s) 215
BceAI ACGGC 2 cut(s) 178, 182
BciT130I CCWGG 1 cut(s) 447
BfmI CTRYAG 1 cut(s) 476
BfoI RGCGCY 1 cut(s) 198
BisI GCNGC 2 cut(s) 217, 229
BlsI GCNGC 2 cut(s) 218, 230
Bme1390I CCNGG 1 cut(s) 447
BmgT120I GGNCC 1 cut(s) 309
BmiI GGNNCC 1 cut(s) 557
BmrFI CCNGG 1 cut(s) 447
BpiI GAAGAC 1 cut(s) 115
Bpu10I CCTNAGC 2 cut(s) 191, 353
BsaHI GRCGYC 1 cut(s) 420
BsaJI CCNNGG 2 cut(s) 446, 544
BsaWI WCCGGW 1 cut(s) 490
Bsc4I CCNNNNNNNGG 1 cut(s) 497
Bse1I ACTGG 1 cut(s) 133
BseBI CCWGG 1 cut(s) 447
BseDI CCNNGG 2 cut(s) 446, 544
BseLI CCNNNNNNNGG 1 cut(s) 497
BseMII CTCAG 1 cut(s) 453
BseNI ACTGG 1 cut(s) 133
BseRI GAGGAG 1 cut(s) 95
BseXI GCAGC 1 cut(s) 215
BshFI GGCC 1 cut(s) 310
BshNI GGYRCC 1 cut(s) 555
BsiHKAI GWGCWC 1 cut(s) 20
BsiSI CCGG 2 cut(s) 491, 510
BslFI GGGAC 1 cut(s) 377
BslI CCNNNNNNNGG 1 cut(s) 497
BsmFI GGGAC 1 cut(s) 377
BsnI GGCC 1 cut(s) 310
Bsp1286I GDGCHC 1 cut(s) 20
Bsp19I CCATGG 1 cut(s) 544
BspACI CCGC 1 cut(s) 216
BspANI GGCC 1 cut(s) 310
BspCNI CTCAG 1 cut(s) 454
BspLI GGNNCC 1 cut(s) 557
BspQI GCTCTTC 1 cut(s) 25
BspT107I GGYRCC 1 cut(s) 555
BsrI ACTGG 1 cut(s) 133
BssECI CCNNGG 2 cut(s) 446, 544
BssNI GRCGYC 1 cut(s) 420
BssT1I CCWWGG 1 cut(s) 544
Bst2UI CCWGG 1 cut(s) 447
Bst4CI ACNGT 4 cut(s) 121, 251, 430, 517
Bst6I CTCTTC 1 cut(s) 25
BstACI GRCGYC 1 cut(s) 420
BstC8I GCNNGC 2 cut(s) 88, 375
BstDEI CTNAG 3 cut(s) 191, 353, 462
BstDSI CCRYGG 1 cut(s) 544
BstH2I RGCGCY 1 cut(s) 198
BstHHI GCGC 2 cut(s) 86, 197
BstMWI GCNNNNNNNGC 1 cut(s) 225
BstNI CCWGG 1 cut(s) 447
BstNSI RCATGY 2 cut(s) 297, 377
BstSCI CCNGG 1 cut(s) 445
BstSFI CTRYAG 1 cut(s) 476
BstV1I GCAGC 1 cut(s) 215
BstV2I GAAGAC 1 cut(s) 115
BstXI CCANNNNNNTGG 1 cut(s) 551
BsuRI GGCC 1 cut(s) 310
BtgI CCRYGG 1 cut(s) 544
BtsI GCAGTG 2 cut(s) 171, 268
BtsIMutI CAGTG 4 cut(s) 171, 268, 426, 459
Cac8I GCNNGC 2 cut(s) 88, 375
CfoI GCGC 2 cut(s) 86, 197
Cfr13I GGNCC 1 cut(s) 309
CseI GACGC 2 cut(s) 49, 428
Csp6I GTAC 1 cut(s) 556
CspCI CAANNNNNGTGG 2 cut(s) 312, 347
CviAII CATG 3 cut(s) 294, 374, 545
CviJI RGCY 7 cut(s) 18, 103, 219, 304, 310, 352, 509
CviKI_1 RGCY 7 cut(s) 18, 103, 219, 304, 310, 352, 509
CviQI GTAC 1 cut(s) 556
DdeI CTNAG 3 cut(s) 191, 353, 462
Eam1104I CTCTTC 1 cut(s) 25
EarI CTCTTC 1 cut(s) 25
Ecl136II GAGCTC 1 cut(s) 18
Eco130I CCWWGG 1 cut(s) 544
Eco24I GRGCYC 1 cut(s) 20
Eco53kI GAGCTC 1 cut(s) 18
EcoICRI GAGCTC 1 cut(s) 18
EcoRII CCWGG 1 cut(s) 445
EcoT14I CCWWGG 1 cut(s) 544
EcoT38I GRGCYC 1 cut(s) 20
ErhI CCWWGG 1 cut(s) 544
FaeI CATG 3 cut(s) 297, 377, 548
FaiI YATR 5 cut(s) 27, 282, 295, 375, 546
FaqI GGGAC 1 cut(s) 377
FatI CATG 3 cut(s) 293, 373, 544
Fnu4HI GCNGC 2 cut(s) 217, 229
FriOI GRGCYC 1 cut(s) 20
Fsp4HI GCNGC 2 cut(s) 217, 229
GlaI GCGC 2 cut(s) 85, 196
GluI GCNGC 2 cut(s) 217, 229
HaeII RGCGCY 1 cut(s) 198
HaeIII GGCC 1 cut(s) 310
HapII CCGG 2 cut(s) 491, 510
HgaI GACGC 2 cut(s) 49, 428
HhaI GCGC 2 cut(s) 86, 197
Hin1I GRCGYC 1 cut(s) 420
Hin1II CATG 3 cut(s) 297, 377, 548
Hin6I GCGC 2 cut(s) 84, 195
HinP1I GCGC 2 cut(s) 84, 195
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HindIII AAGCTT 1 cut(s) 350
HinfI GANTC 2 cut(s) 455, 575
HpaII CCGG 2 cut(s) 491, 510
Hpy166II GTNNAC 2 cut(s) 181, 459
Hpy188I TCNGA 1 cut(s) 574
Hpy188III TCNNGA 2 cut(s) 109, 151
Hpy8I GTNNAC 2 cut(s) 181, 459
Hpy99I CGWCG 2 cut(s) 319, 565
HpyAV CCTTC 2 cut(s) 388, 460
HpyCH4III ACNGT 4 cut(s) 121, 251, 430, 517
HpyCH4V TGCA 5 cut(s) 171, 297, 373, 401, 503
HpyF10VI GCNNNNNNNGC 1 cut(s) 225
HpyF3I CTNAG 3 cut(s) 191, 353, 462
Hsp92I GRCGYC 1 cut(s) 420
Hsp92II CATG 3 cut(s) 297, 377, 548
HspAI GCGC 2 cut(s) 84, 195
KpnI GGTACC 1 cut(s) 559
LguI GCTCTTC 1 cut(s) 25
LmnI GCTCC 1 cut(s) 108
Lsp1109I GCAGC 1 cut(s) 215
MaeIII GTNAC 2 cut(s) 251, 408
MboII GAAGA 3 cut(s) 12, 115, 228
MhlI GDGCHC 1 cut(s) 20
MluCI AATT 1 cut(s) 549
MlyI GAGTC 1 cut(s) 464
MnlI CCTC 5 cut(s) 104, 116, 149, 322, 498
MseI TTAA 2 cut(s) 77, 580
MspI CCGG 2 cut(s) 491, 510
MspR9I CCNGG 1 cut(s) 447
MvaI CCWGG 1 cut(s) 447
MwoI GCNNNNNNNGC 1 cut(s) 225
NcoI CCATGG 1 cut(s) 544
NlaIII CATG 3 cut(s) 297, 377, 548
NlaIV GGNNCC 1 cut(s) 557
NmuCI GTSAC 1 cut(s) 408
NspI RCATGY 2 cut(s) 297, 377
PaeI GCATGC 1 cut(s) 377
PciSI GCTCTTC 1 cut(s) 25
PfeI GAWTC 1 cut(s) 575
PkrI GCNGC 2 cut(s) 218, 230
PleI GAGTC 1 cut(s) 463
PpsI GAGTC 1 cut(s) 463
Psp124BI GAGCTC 1 cut(s) 20
Psp6I CCWGG 1 cut(s) 445
PspGI CCWGG 1 cut(s) 445
PspN4I GGNNCC 1 cut(s) 557
PspPI GGNCC 1 cut(s) 309
RsaI GTAC 1 cut(s) 557
RsaNI GTAC 1 cut(s) 556
SacI GAGCTC 1 cut(s) 20
SapI GCTCTTC 1 cut(s) 25
SaqAI TTAA 2 cut(s) 77, 580
SatI GCNGC 2 cut(s) 217, 229
Sau96I GGNCC 1 cut(s) 309
SchI GAGTC 1 cut(s) 464
ScrFI CCNGG 1 cut(s) 447
SduI GDGCHC 1 cut(s) 20
SetI ASST 9 cut(s) 20, 96, 105, 192, 354, 359, 437, 471, 483
SfcI CTRYAG 1 cut(s) 476
SphI GCATGC 1 cut(s) 377
Sse9I AATT 1 cut(s) 549
SsiI CCGC 1 cut(s) 216
SstI GAGCTC 1 cut(s) 20
StyD4I CCNGG 1 cut(s) 445
StyI CCWWGG 1 cut(s) 544
TaaI ACNGT 4 cut(s) 121, 251, 430, 517
TaqI TCGA 2 cut(s) 314, 453
TasI AATT 1 cut(s) 549
TauI GCSGC 1 cut(s) 219
TfiI GAWTC 1 cut(s) 575
Tru1I TTAA 2 cut(s) 77, 580
Tru9I TTAA 2 cut(s) 77, 580
TscAI CASTG 4 cut(s) 178, 268, 433, 466
TseFI GTSAC 1 cut(s) 408
TseI GCWGC 1 cut(s) 228
Tsp45I GTSAC 1 cut(s) 408
TspDTI ATGAA 1 cut(s) 282
TspRI CASTG 4 cut(s) 178, 268, 433, 466
XceI RCATGY 2 cut(s) 297, 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.