Rroxscaffold_5G00362870

Protein of unknown function (DUF679)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0003054
Physical Location & Seq
Reverse (-)
43927044 .. 43927652
609 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00362870.1

Sequence Viewer

Length: 609 bp
ATGGGGGCTTCATCCAGCTCTTCATCCAAATCCAAGAACTCCACCAAAAACACGGGTGTCAAAGACACTGCATTAACAGGAATAGGCAACCTCATCAAGCTCCTCCCTACCGGCACGGTATTCTTGTTCCAGTTCCTCAACCCCGTTTTAACCAACAATGGCGAATGCAACATGTCCGTCAACAAGATTCTAAGCGCCATTCTCATTATCGTTTCCGCCTTCTCTTGCTGCTTTGCTTCCTTCACTGACAGTTACACCGGCAGTGATGGACAAATTCACTATGGAATTGCAACAACAAAGGGTCTTTGGCCCTCAACCAGTTCTGGCTCTGTGGACTTGTCTGCTTACAAGCTTCGGGTTGGGGACTTTGTGCATGCCTTCTTTTCCTTAATCGTGTTTGCAGGGCTATCACTTTTGAATTCCGACACTGTGAGGTGCTTCTATCCTGCATTTGAATCCACTGAGAAGGTTCTGCTCCAGGCGTTGCCCCCTGTAATAGGCACTATTGCTGGTGCCGTCTTTGTTCTGTTCCCTAATACACGCCACGGAATTGGGTACCCTTATTCAAGTTCTGATTCTTCACAAGACTCGGACTCGGAGGACGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF679

Protein Analysis

202

Amino Acids

21.34

Weight (kDa)

5.84

Isoelectric Point (pI)

38.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF679 PF05078 27 - 186 1.6e-62 Protein of unknown function (DUF679)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 555
AccB1I GGYRCC 2 cut(s) 512, 555
AciI CCGC 1 cut(s) 216
AcsI RAATTY 2 cut(s) 273, 418
AcyI GRCGYC 1 cut(s) 603
AfaI GTAC 1 cut(s) 557
AfiI CCNNNNNNNGG 1 cut(s) 497
AflIII ACRYGT 1 cut(s) 171
AgsI TTSAA 3 cut(s) 418, 455, 567
AjnI CCWGG 1 cut(s) 477
AluBI AGCT 3 cut(s) 18, 100, 352
AluI AGCT 3 cut(s) 18, 100, 352
AoxI GGCC 1 cut(s) 308
ApeKI GCWGC 1 cut(s) 228
ApoI RAATTY 2 cut(s) 273, 418
Asp718I GGTACC 1 cut(s) 555
AspLEI GCGC 1 cut(s) 197
AspS9I GGNCC 1 cut(s) 309
BanI GGYRCC 2 cut(s) 512, 555
BbvI GCAGC 1 cut(s) 215
BccI CCATC 1 cut(s) 260
BceAI ACGGC 1 cut(s) 500
BciT130I CCWGG 1 cut(s) 479
BfoI RGCGCY 1 cut(s) 198
BisI GCNGC 1 cut(s) 229
BlsI GCNGC 1 cut(s) 230
Bme1390I CCNGG 1 cut(s) 479
BmgT120I GGNCC 1 cut(s) 309
BmiI GGNNCC 2 cut(s) 514, 557
BmrFI CCNGG 1 cut(s) 479
BpmI CTGGAG 1 cut(s) 461
BsaHI GRCGYC 1 cut(s) 603
BsaJI CCNNGG 1 cut(s) 544
Bsc4I CCNNNNNNNGG 1 cut(s) 497
Bse118I RCCGGY 2 cut(s) 110, 257
Bse1I ACTGG 2 cut(s) 130, 318
BseBI CCWGG 1 cut(s) 479
BseDI CCNNGG 1 cut(s) 544
BseGI GGATG 2 cut(s) 11, 23
BseLI CCNNNNNNNGG 1 cut(s) 497
BseMII CTCAG 1 cut(s) 453
BseNI ACTGG 2 cut(s) 130, 318
BseRI GAGGAG 1 cut(s) 92
BseXI GCAGC 1 cut(s) 215
BshFI GGCC 1 cut(s) 310
BshNI GGYRCC 2 cut(s) 512, 555
BsiSI CCGG 2 cut(s) 111, 258
BslFI GGGAC 1 cut(s) 377
BslI CCNNNNNNNGG 1 cut(s) 497
BsmFI GGGAC 1 cut(s) 377
BsmI GAATGC 1 cut(s) 170
BsnI GGCC 1 cut(s) 310
BspACI CCGC 1 cut(s) 216
BspANI GGCC 1 cut(s) 310
BspCNI CTCAG 1 cut(s) 454
BspLI GGNNCC 2 cut(s) 514, 557
BspQI GCTCTTC 1 cut(s) 25
BspT107I GGYRCC 2 cut(s) 512, 555
BsrFI RCCGGY 2 cut(s) 110, 257
BsrI ACTGG 2 cut(s) 130, 318
BssAI RCCGGY 2 cut(s) 110, 257
BssECI CCNNGG 1 cut(s) 544
BssNI GRCGYC 1 cut(s) 603
Bst2UI CCWGG 1 cut(s) 479
Bst4CI ACNGT 3 cut(s) 118, 251, 430
Bst6I CTCTTC 1 cut(s) 25
BstACI GRCGYC 1 cut(s) 603
BstC8I GCNNGC 1 cut(s) 375
BstDEI CTNAG 2 cut(s) 191, 462
BstDSI CCRYGG 1 cut(s) 544
BstENI CCTNNNNNAGG 1 cut(s) 495
BstF5I GGATG 2 cut(s) 11, 23
BstH2I RGCGCY 1 cut(s) 198
BstHHI GCGC 1 cut(s) 197
BstNI CCWGG 1 cut(s) 479
BstNSI RCATGY 2 cut(s) 175, 377
BstSCI CCNGG 1 cut(s) 477
BstV1I GCAGC 1 cut(s) 215
BstXI CCANNNNNNTGG 1 cut(s) 551
BsuRI GGCC 1 cut(s) 310
BtgI CCRYGG 1 cut(s) 544
BtsCI GGATG 2 cut(s) 11, 23
BtsI GCAGTG 2 cut(s) 66, 268
BtsIMutI CAGTG 5 cut(s) 66, 243, 268, 426, 459
Cac8I GCNNGC 1 cut(s) 375
CfoI GCGC 1 cut(s) 197
Cfr10I RCCGGY 2 cut(s) 110, 257
Cfr13I GGNCC 1 cut(s) 309
Csp6I GTAC 1 cut(s) 556
CviAII CATG 2 cut(s) 172, 374
CviJI RGCY 7 cut(s) 8, 18, 100, 310, 327, 352, 406
CviKI_1 RGCY 7 cut(s) 8, 18, 100, 310, 327, 352, 406
CviQI GTAC 1 cut(s) 556
DdeI CTNAG 2 cut(s) 191, 462
Eam1104I CTCTTC 1 cut(s) 25
EarI CTCTTC 1 cut(s) 25
EciI GGCGGA 1 cut(s) 205
EcoNI CCTNNNNNAGG 1 cut(s) 495
EcoRI GAATTC 1 cut(s) 418
EcoRII CCWGG 1 cut(s) 477
FaeI CATG 2 cut(s) 175, 377
FaiI YATR 3 cut(s) 173, 282, 375
FaqI GGGAC 1 cut(s) 377
FatI CATG 2 cut(s) 171, 373
Fnu4HI GCNGC 1 cut(s) 229
FokI GGATG 1 cut(s) 10
Fsp4HI GCNGC 1 cut(s) 229
GlaI GCGC 1 cut(s) 196
GluI GCNGC 1 cut(s) 229
GsuI CTGGAG 1 cut(s) 461
HaeII RGCGCY 1 cut(s) 198
HaeIII GGCC 1 cut(s) 310
HapII CCGG 2 cut(s) 111, 258
HhaI GCGC 1 cut(s) 197
Hin1I GRCGYC 1 cut(s) 603
Hin1II CATG 2 cut(s) 175, 377
Hin6I GCGC 1 cut(s) 195
HinP1I GCGC 1 cut(s) 195
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HindIII AAGCTT 1 cut(s) 350
HinfI GANTC 5 cut(s) 187, 455, 575, 587, 593
HpaII CCGG 2 cut(s) 111, 258
Hpy166II GTNNAC 2 cut(s) 181, 334
Hpy188I TCNGA 4 cut(s) 424, 574, 592, 598
Hpy8I GTNNAC 2 cut(s) 181, 334
HpyAV CCTTC 4 cut(s) 229, 250, 388, 460
HpyCH4III ACNGT 3 cut(s) 118, 251, 430
HpyCH4V TGCA 6 cut(s) 71, 168, 290, 373, 401, 449
HpyF3I CTNAG 2 cut(s) 191, 462
Hsp92I GRCGYC 1 cut(s) 603
Hsp92II CATG 2 cut(s) 175, 377
HspAI GCGC 1 cut(s) 195
KpnI GGTACC 1 cut(s) 559
LguI GCTCTTC 1 cut(s) 25
LmnI GCTCC 2 cut(s) 105, 480
Lsp1109I GCAGC 1 cut(s) 215
MaeIII GTNAC 1 cut(s) 251
MboII GAAGA 2 cut(s) 12, 570
MluCI AATT 4 cut(s) 273, 285, 418, 549
MlyI GAGTC 2 cut(s) 581, 587
MmeI TCCRAC 1 cut(s) 447
MnlI CCTC 6 cut(s) 101, 113, 146, 322, 426, 592
MseI TTAA 3 cut(s) 74, 149, 389
MspI CCGG 2 cut(s) 111, 258
MspR9I CCNGG 1 cut(s) 479
Mva1269I GAATGC 1 cut(s) 170
MvaI CCWGG 1 cut(s) 479
NlaIII CATG 2 cut(s) 175, 377
NlaIV GGNNCC 2 cut(s) 514, 557
NspI RCATGY 2 cut(s) 175, 377
PaeI GCATGC 1 cut(s) 377
PciI ACATGT 1 cut(s) 171
PciSI GCTCTTC 1 cut(s) 25
PctI GAATGC 1 cut(s) 170
PfeI GAWTC 3 cut(s) 187, 455, 575
PkrI GCNGC 1 cut(s) 230
PleI GAGTC 2 cut(s) 581, 587
PpsI GAGTC 2 cut(s) 581, 587
PscI ACATGT 1 cut(s) 171
Psp6I CCWGG 1 cut(s) 477
PspGI CCWGG 1 cut(s) 477
PspN4I GGNNCC 2 cut(s) 514, 557
PspPI GGNCC 1 cut(s) 309
RsaI GTAC 1 cut(s) 557
RsaNI GTAC 1 cut(s) 556
SapI GCTCTTC 1 cut(s) 25
SaqAI TTAA 3 cut(s) 74, 149, 389
SatI GCNGC 1 cut(s) 229
Sau96I GGNCC 1 cut(s) 309
SchI GAGTC 2 cut(s) 581, 587
ScrFI CCNGG 1 cut(s) 479
SetI ASST 6 cut(s) 20, 93, 102, 354, 437, 471
SphI GCATGC 1 cut(s) 377
Sse9I AATT 4 cut(s) 273, 285, 418, 549
SsiI CCGC 1 cut(s) 216
StyD4I CCNGG 1 cut(s) 477
TaaI ACNGT 3 cut(s) 118, 251, 430
TasI AATT 4 cut(s) 273, 285, 418, 549
TfiI GAWTC 3 cut(s) 187, 455, 575
Tru1I TTAA 3 cut(s) 74, 149, 389
Tru9I TTAA 3 cut(s) 74, 149, 389
TscAI CASTG 5 cut(s) 73, 250, 268, 433, 466
TseI GCWGC 1 cut(s) 228
TspDTI ATGAA 1 cut(s) 12
TspGWI ACGGA 2 cut(s) 166, 561
TspRI CASTG 5 cut(s) 73, 250, 268, 433, 466
XagI CCTNNNNNAGG 1 cut(s) 495
XapI RAATTY 2 cut(s) 273, 418
XceI RCATGY 2 cut(s) 175, 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.