FvH4_4g16710

tRNA rRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0015140
Physical Location & Seq
Forward (+)
20562102 .. 20565722
3621 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g16710.t1

Sequence Viewer

Length: 660 bp
ATGGTGGTGGAGAGCTATGTGGTGGTTCACAACATAGCCAAGAGGCACAACGTGGGCACACTCGCCCGAAGCGCCACCGCCTTCGGTGTCTCCGAGCTCATTCTCGTTGGCCGCAGAGAATTCAACGCCTTCGGCAGCCACGGCTCCACCTCCCACGTTCGCTTCCGCCACTTCCACTCTCTCCAAGACGCCCGCCAATTCCTCAAGGACAGAGATTGTGATATCTGCGGCGTTGAGATCACTGATAATGCTATGCCTGTCAATCACCACCCTTTTACCAAAAGCACTGCTTTTCTCCTTGGCAATGAGGGAACAGGGCTTTCTGCTAAGGAGTATGAGATATGTGATTTCTTTGTGTATATCCCACAGTATGGGGACGGCACGGCTTCCTTGAATGTTACGGTCGCAGCTTCCATTGTCCTGCATCAGTTTGGAGTCTGGGCTGGCTTCCCTGAGAGAACCCGTGATGGGAACAAGTTCATTGTGGCACAGAAACCTGCAAAGCAGACAAGGCGAAGTTTCTGTGCAGAAACAGCAGATTCCGTAATTGAAGAGCGAAAATCTAGAAAAGAGCATAACTCTAATGGTTTCTTTGACGAGAATGCAAATGACAATCCATCTTCTAACCTTCTGGATGGTTTGTTTAGTGATGTACGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SpoU_methylase

Protein Analysis

220

Amino Acids

24.37

Weight (kDa)

6.55

Isoelectric Point (pI)

42.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SpoU_methylase PF00588 6 - 142 1.6e-31 SpoU rRNA Methylase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015140)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15520
fragaria_vesca FvH4_4g16710
malus_domestica MD13G1177300.v1.1
prunus_persica Prupe.1G146500_v2.0.a1 Prupe.1G146500_v2.0.a1
pyrus_communis pycom13g15280 pycom16g15030
rosa_chinensis RchiOBHm_Chr4g0420161
rosa_laevigata RLG00000007785
rosa_multiflora Rmu_sc0004455.1_g000001
rosa_roxburghii Rroxscaffold_5G00362860
rosa_rugosa Rorug04G0162700
rosa_samantha Rh4AG223500 Rh4BG226100 Rh4DG222300
rosa_wichuraiana Rw4G019240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 505
AccB7I CCANNNNNTGG 1 cut(s) 371
AciI CCGC 5 cut(s) 78, 112, 166, 193, 228
AcoI YGGCCR 1 cut(s) 109
AcsI RAATTY 1 cut(s) 119
AcyI GRCGYC 1 cut(s) 189
AdeI CACNNNGTG 1 cut(s) 52
AfaI GTAC 1 cut(s) 654
AfiI CCNNNNNNNGG 2 cut(s) 371, 468
AgsI TTSAA 3 cut(s) 124, 394, 551
AluBI AGCT 3 cut(s) 15, 97, 410
AluI AGCT 3 cut(s) 15, 97, 410
Alw21I GWGCWC 1 cut(s) 99
Alw26I GTCTC 1 cut(s) 94
AoxI GGCC 1 cut(s) 109
ApeKI GCWGC 2 cut(s) 135, 407
ApoI RAATTY 1 cut(s) 119
Asp700I GAANNNNTTC 1 cut(s) 476
AspLEI GCGC 1 cut(s) 74
AsuHPI GGTGA 1 cut(s) 257
BaeGI GKGCMC 1 cut(s) 59
BanII GRGCYC 1 cut(s) 99
Bbv12I GWGCWC 1 cut(s) 99
BbvI GCAGC 2 cut(s) 147, 419
BccI CCATC 3 cut(s) 461, 625, 629
BceAI ACGGC 3 cut(s) 157, 394, 399
BcoDI GTCTC 1 cut(s) 94
BfaI CTAG 1 cut(s) 564
BfoI RGCGCY 1 cut(s) 75
BfuAI ACCTGC 1 cut(s) 505
BisI GCNGC 4 cut(s) 112, 136, 229, 408
BlsI GCNGC 4 cut(s) 113, 137, 230, 409
BmiI GGNNCC 1 cut(s) 145
BmsI GCATC 1 cut(s) 433
BplI GAGNNNNNCTC 2 cut(s) 563, 595
Bpu10I CCTNAGC 1 cut(s) 327
BpuEI CTTGAG 1 cut(s) 188
BsaHI GRCGYC 1 cut(s) 189
BsaJI CCNNGG 2 cut(s) 139, 298
Bsc4I CCNNNNNNNGG 2 cut(s) 371, 468
Bse3DI GCAATG 1 cut(s) 310
BseDI CCNNGG 2 cut(s) 139, 298
BseGI GGATG 1 cut(s) 640
BseLI CCNNNNNNNGG 2 cut(s) 371, 468
BseMI GCAATG 1 cut(s) 310
BseMII CTCAG 1 cut(s) 444
BseSI GKGCMC 1 cut(s) 59
BseXI GCAGC 2 cut(s) 147, 419
BsgI GTGCAG 1 cut(s) 546
Bsh1285I CGRYCG 1 cut(s) 405
BshFI GGCC 1 cut(s) 111
BsiEI CGRYCG 1 cut(s) 405
BsiHKAI GWGCWC 1 cut(s) 99
BslFI GGGAC 1 cut(s) 389
BslI CCNNNNNNNGG 2 cut(s) 371, 468
BsmAI GTCTC 1 cut(s) 94
BsmFI GGGAC 1 cut(s) 389
BsmI GAATGC 1 cut(s) 607
BsnI GGCC 1 cut(s) 111
Bsp1286I GDGCHC 2 cut(s) 59, 99
Bsp143I GATC 1 cut(s) 237
BspACI CCGC 5 cut(s) 78, 112, 166, 193, 228
BspANI GGCC 1 cut(s) 111
BspCNI CTCAG 1 cut(s) 445
BspLI GGNNCC 1 cut(s) 145
BspMI ACCTGC 1 cut(s) 505
BspQI GCTCTTC 1 cut(s) 546
BsrDI GCAATG 1 cut(s) 310
BssECI CCNNGG 2 cut(s) 139, 298
BssMI GATC 1 cut(s) 237
BssNI GRCGYC 1 cut(s) 189
BssT1I CCWWGG 1 cut(s) 298
Bst4CI ACNGT 2 cut(s) 369, 403
Bst6I CTCTTC 1 cut(s) 546
BstACI GRCGYC 1 cut(s) 189
BstC8I GCNNGC 2 cut(s) 193, 445
BstDEI CTNAG 2 cut(s) 327, 453
BstDSI CCRYGG 1 cut(s) 139
BstF5I GGATG 1 cut(s) 640
BstH2I RGCGCY 1 cut(s) 75
BstHHI GCGC 1 cut(s) 74
BstKTI GATC 1 cut(s) 240
BstMAI GTCTC 1 cut(s) 94
BstMBI GATC 1 cut(s) 237
BstMCI CGRYCG 1 cut(s) 405
BstMWI GCNNNNNNNGC 4 cut(s) 71, 141, 511, 533
BstSLI GKGCMC 1 cut(s) 59
BstV1I GCAGC 2 cut(s) 147, 419
BsuRI GGCC 1 cut(s) 111
BtgI CCRYGG 1 cut(s) 139
BtsCI GGATG 1 cut(s) 640
BtsI GCAGTG 1 cut(s) 285
BtsIMutI CAGTG 2 cut(s) 240, 285
BveI ACCTGC 1 cut(s) 505
Cac8I GCNNGC 2 cut(s) 193, 445
CfoI GCGC 1 cut(s) 74
CseI GACGC 1 cut(s) 197
Csp6I GTAC 1 cut(s) 653
CviQI GTAC 1 cut(s) 653
DdeI CTNAG 2 cut(s) 327, 453
DpnI GATC 1 cut(s) 239
DpnII GATC 1 cut(s) 237
DraIII CACNNNGTG 1 cut(s) 52
EaeI YGGCCR 1 cut(s) 109
Eam1104I CTCTTC 1 cut(s) 546
EarI CTCTTC 1 cut(s) 546
EciI GGCGGA 1 cut(s) 155
Ecl136II GAGCTC 1 cut(s) 97
Eco130I CCWWGG 1 cut(s) 298
Eco24I GRGCYC 1 cut(s) 99
Eco32I GATATC 1 cut(s) 223
Eco53kI GAGCTC 1 cut(s) 97
EcoICRI GAGCTC 1 cut(s) 97
EcoRI GAATTC 1 cut(s) 119
EcoRV GATATC 1 cut(s) 223
EcoT14I CCWWGG 1 cut(s) 298
EcoT38I GRGCYC 1 cut(s) 99
ErhI CCWWGG 1 cut(s) 298
FaiI YATR 8 cut(s) 18, 35, 254, 336, 343, 360, 372, 576
FalI AAGNNNNNCTT 2 cut(s) 274, 306
FaqI GGGAC 1 cut(s) 389
FauI CCCGC 1 cut(s) 200
Fnu4HI GCNGC 4 cut(s) 112, 136, 229, 408
FokI GGATG 1 cut(s) 647
FriOI GRGCYC 1 cut(s) 99
Fsp4HI GCNGC 4 cut(s) 112, 136, 229, 408
FspBI CTAG 1 cut(s) 564
GlaI GCGC 1 cut(s) 73
GluI GCNGC 4 cut(s) 112, 136, 229, 408
HaeII RGCGCY 1 cut(s) 75
HaeIII GGCC 1 cut(s) 111
HgaI GACGC 1 cut(s) 197
HhaI GCGC 1 cut(s) 74
Hin1I GRCGYC 1 cut(s) 189
Hin6I GCGC 1 cut(s) 72
HinP1I GCGC 1 cut(s) 72
HinfI GANTC 2 cut(s) 435, 539
HphI GGTGA 1 cut(s) 257
Hpy166II GTNNAC 1 cut(s) 28
Hpy188I TCNGA 1 cut(s) 94
Hpy188III TCNNGA 2 cut(s) 564, 632
Hpy8I GTNNAC 1 cut(s) 28
HpyAV CCTTC 3 cut(s) 91, 139, 638
HpyCH4III ACNGT 2 cut(s) 369, 403
HpyCH4IV ACGT 2 cut(s) 51, 156
HpyCH4V TGCA 4 cut(s) 424, 500, 527, 605
HpyF10VI GCNNNNNNNGC 4 cut(s) 71, 141, 511, 533
HpyF3I CTNAG 2 cut(s) 327, 453
HpySE526I ACGT 2 cut(s) 51, 156
Hsp92I GRCGYC 1 cut(s) 189
HspAI GCGC 1 cut(s) 72
Kzo9I GATC 1 cut(s) 237
LguI GCTCTTC 1 cut(s) 546
LmnI GCTCC 1 cut(s) 149
LpnPI CCDG 8 cut(s) 270, 300, 424, 429, 434, 465, 510, 617
Lsp1109I GCAGC 2 cut(s) 147, 419
LweI GCATC 1 cut(s) 433
MaeI CTAG 1 cut(s) 564
MaeII ACGT 2 cut(s) 51, 156
MaeIII GTNAC 1 cut(s) 397
MalI GATC 1 cut(s) 239
MboI GATC 1 cut(s) 237
MboII GAAGA 2 cut(s) 563, 612
MhlI GDGCHC 2 cut(s) 59, 99
MluCI AATT 3 cut(s) 119, 197, 546
MlyI GAGTC 1 cut(s) 444
MnlI CCTC 4 cut(s) 36, 160, 212, 301
MroXI GAANNNNTTC 1 cut(s) 476
Mva1269I GAATGC 1 cut(s) 607
MwoI GCNNNNNNNGC 4 cut(s) 71, 141, 511, 533
NdeII GATC 1 cut(s) 237
NlaIV GGNNCC 1 cut(s) 145
PciSI GCTCTTC 1 cut(s) 546
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PctI GAATGC 1 cut(s) 607
PdmI GAANNNNTTC 1 cut(s) 476
PfeI GAWTC 1 cut(s) 539
PflMI CCANNNNNTGG 1 cut(s) 371
PkrI GCNGC 4 cut(s) 113, 137, 230, 409
PleI GAGTC 1 cut(s) 443
PpsI GAGTC 1 cut(s) 443
Psp124BI GAGCTC 1 cut(s) 99
PspN4I GGNNCC 1 cut(s) 145
RsaI GTAC 1 cut(s) 654
RsaNI GTAC 1 cut(s) 653
SacI GAGCTC 1 cut(s) 99
SapI GCTCTTC 1 cut(s) 546
SatI GCNGC 4 cut(s) 112, 136, 229, 408
Sau3AI GATC 1 cut(s) 237
SchI GAGTC 1 cut(s) 444
SduI GDGCHC 2 cut(s) 59, 99
SetI ASST 8 cut(s) 17, 54, 99, 152, 159, 412, 499, 630
SfaNI GCATC 1 cut(s) 433
SmlI CTYRAG 1 cut(s) 203
SmoI CTYRAG 1 cut(s) 203
Sse9I AATT 3 cut(s) 119, 197, 546
SsiI CCGC 5 cut(s) 78, 112, 166, 193, 228
SspMI CTAG 1 cut(s) 564
SstI GAGCTC 1 cut(s) 99
StyI CCWWGG 1 cut(s) 298
TaaI ACNGT 2 cut(s) 369, 403
TaiI ACGT 2 cut(s) 54, 159
TasI AATT 3 cut(s) 119, 197, 546
TauI GCSGC 2 cut(s) 114, 231
TfiI GAWTC 1 cut(s) 539
TscAI CASTG 2 cut(s) 247, 292
TseI GCWGC 2 cut(s) 135, 407
TspDTI ATGAA 1 cut(s) 469
TspGWI ACGGA 1 cut(s) 532
TspRI CASTG 2 cut(s) 247, 292
Van91I CCANNNNNTGG 1 cut(s) 371
XapI RAATTY 1 cut(s) 119
XbaI TCTAGA 1 cut(s) 563
XmnI GAANNNNTTC 1 cut(s) 476
XspI CTAG 1 cut(s) 564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.