AT4G16410

Protein of unknown function (DUF751)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0015477
Physical Location & Seq
Reverse (-)
9261866 .. 9262601
736 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G16410.1

Sequence Viewer

Length: 558 bp
ATGACGATGATGTCTCTCCCCAAAATGAGACTATCTTGTCTGCAACCACACCTCCATTTTACCAGTAGCAGATCAATAGCAGCAGCTCTAGCTTTTCCAAGCTCAAGCATAAGGAACAGAACATCATGTCTTGTTCGGTGTGGCTCAGCCAGAAGAAGATTGACTACGAAAAGTAGCAGAAACAGCAAGCTTGAAAGAGCACAACCTCCTCCTCTGGGTTTGGAACGGCACTGGAGATATGCTGTGGTTGGTGGAGTCTCTGTAGGGTTGATGATGGTTTTGGTATTGGGAATGGATGCAGAGAAAGCTATGGCGCTTGGACCAGAAGGTCCTTTAATGGAAGAGTTTTGGGACAACGTTAGAAGGTACGGTCTTTACGCTCTCACGGTTAGCACTGGAGCTCTCTCTGCTGTCTTTGAGCCCATCTTTGAGCTCCTCAAGAACCCAATCTCCGCCATTCTCATTCTACTCATCTTAGGTGGTAGCTTCTATATCGTCTCTCAAGTTGTTTCTGCCATGATTGGTGTCAACGAATTCGCTTATCAATACAGTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF751

Protein Analysis

185

Amino Acids

20.41

Weight (kDa)

9.76

Isoelectric Point (pI)

56.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF751 PF05421 116 - 175 9.4e-22 Protein of unknown function (DUF751)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015477)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16410
fragaria_vesca FvH4_3g00530
malus_domestica MD00G1057700.v1.1 MD10G1337000.v1.1
prunus_persica Prupe.4G006400_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0000731
rosa_laevigata RLG00000030890
rosa_multiflora Rmu_sc0000255.1_g000003 Rmu_sc0011992.1_g000006
rosa_roxburghii Rroxscaffold_1G00075500
rosa_rugosa Rorug04G0386100
rosa_samantha Rh5AG006000 Rh5BG007800 Rh5CG006500 Rh5DG006500
rosa_wichuraiana Rw5G000610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 10, 36, 327
AciI CCGC 1 cut(s) 453
AclI AACGTT 1 cut(s) 357
AcsI RAATTY 1 cut(s) 533
AfaI GTAC 1 cut(s) 368
AfiI CCNNNNNNNGG 1 cut(s) 215
AgsI TTSAA 1 cut(s) 194
AloI GAACNNNNNNTCC 2 cut(s) 434, 466
AluBI AGCT 8 cut(s) 86, 92, 102, 190, 308, 401, 433, 486
AluI AGCT 8 cut(s) 86, 92, 102, 190, 308, 401, 433, 486
Alw21I GWGCWC 3 cut(s) 202, 403, 435
Alw26I GTCTC 4 cut(s) 18, 22, 262, 502
ApeKI GCWGC 2 cut(s) 80, 83
ApoI RAATTY 1 cut(s) 533
AspLEI GCGC 1 cut(s) 316
AspS9I GGNCC 2 cut(s) 320, 329
AvaII GGWCC 2 cut(s) 320, 329
BanII GRGCYC 3 cut(s) 403, 423, 435
BarI GAAGNNNNNNTAC 2 cut(s) 148, 180
Bbv12I GWGCWC 3 cut(s) 202, 403, 435
BbvI GCAGC 2 cut(s) 92, 95
BccI CCATC 2 cut(s) 268, 431
BceAI ACGGC 1 cut(s) 242
BcoDI GTCTC 4 cut(s) 18, 22, 262, 502
BfaI CTAG 1 cut(s) 89
BfmI CTRYAG 1 cut(s) 261
BfoI RGCGCY 1 cut(s) 317
BisI GCNGC 2 cut(s) 81, 84
BlpI GCTNAGC 1 cut(s) 145
BlsI GCNGC 2 cut(s) 82, 85
Bme18I GGWCC 2 cut(s) 320, 329
BmgT120I GGNCC 2 cut(s) 320, 329
BmsI GCATC 1 cut(s) 286
BpmI CTGGAG 2 cut(s) 253, 417
Bpu1102I GCTNAGC 1 cut(s) 145
BpuEI CTTGAG 3 cut(s) 88, 422, 486
BsaXI ACNNNNNCTCC 6 cut(s) 36, 66, 246, 276, 434, 464
Bsc4I CCNNNNNNNGG 1 cut(s) 215
Bse1I ACTGG 3 cut(s) 63, 236, 400
BseGI GGATG 1 cut(s) 301
BseLI CCNNNNNNNGG 1 cut(s) 215
BseMII CTCAG 1 cut(s) 159
BseNI ACTGG 3 cut(s) 63, 236, 400
BseRI GAGGAG 3 cut(s) 198, 201, 425
BseXI GCAGC 2 cut(s) 92, 95
BsiHKAI GWGCWC 3 cut(s) 202, 403, 435
BslFI GGGAC 1 cut(s) 365
BslI CCNNNNNNNGG 1 cut(s) 215
BsmAI GTCTC 4 cut(s) 18, 22, 262, 502
BsmBI CGTCTC 1 cut(s) 502
BsmFI GGGAC 1 cut(s) 365
Bsp1286I GDGCHC 4 cut(s) 202, 403, 423, 435
Bsp143I GATC 1 cut(s) 71
Bsp1720I GCTNAGC 1 cut(s) 145
BspACI CCGC 1 cut(s) 453
BspCNI CTCAG 1 cut(s) 158
BsrI ACTGG 3 cut(s) 63, 236, 400
BssMI GATC 1 cut(s) 71
Bst4CI ACNGT 3 cut(s) 371, 388, 551
Bst6I CTCTTC 1 cut(s) 336
BstC8I GCNNGC 1 cut(s) 188
BstDEI CTNAG 2 cut(s) 145, 475
BstF5I GGATG 1 cut(s) 301
BstH2I RGCGCY 1 cut(s) 317
BstHHI GCGC 1 cut(s) 316
BstKTI GATC 1 cut(s) 74
BstMAI GTCTC 4 cut(s) 18, 22, 262, 502
BstMBI GATC 1 cut(s) 71
BstMWI GCNNNNNNNGC 4 cut(s) 89, 183, 305, 407
BstSFI CTRYAG 1 cut(s) 261
BstV1I GCAGC 2 cut(s) 92, 95
BtsCI GGATG 1 cut(s) 301
BtsIMutI CAGTG 2 cut(s) 229, 393
Cac8I GCNNGC 1 cut(s) 188
CfoI GCGC 1 cut(s) 316
Cfr13I GGNCC 2 cut(s) 320, 329
Csp6I GTAC 1 cut(s) 367
CviAII CATG 2 cut(s) 126, 517
CviQI GTAC 1 cut(s) 367
DdeI CTNAG 2 cut(s) 145, 475
DpnI GATC 1 cut(s) 73
DpnII GATC 1 cut(s) 71
DrdI GACNNNNNNGTC 3 cut(s) 10, 36, 327
DseDI GACNNNNNNGTC 3 cut(s) 10, 36, 327
Eam1104I CTCTTC 1 cut(s) 336
EarI CTCTTC 1 cut(s) 336
EciI GGCGGA 1 cut(s) 442
Ecl136II GAGCTC 2 cut(s) 401, 433
Eco24I GRGCYC 3 cut(s) 403, 423, 435
Eco47I GGWCC 2 cut(s) 320, 329
Eco53kI GAGCTC 2 cut(s) 401, 433
EcoICRI GAGCTC 2 cut(s) 401, 433
EcoO109I RGGNCCY 1 cut(s) 329
EcoRI GAATTC 1 cut(s) 533
EcoT38I GRGCYC 3 cut(s) 403, 423, 435
Esp3I CGTCTC 1 cut(s) 502
FaeI CATG 2 cut(s) 129, 520
FaiI YATR 6 cut(s) 110, 127, 240, 311, 492, 518
FaqI GGGAC 1 cut(s) 365
FatI CATG 2 cut(s) 125, 516
Fnu4HI GCNGC 2 cut(s) 81, 84
FokI GGATG 1 cut(s) 308
FriOI GRGCYC 3 cut(s) 403, 423, 435
Fsp4HI GCNGC 2 cut(s) 81, 84
FspBI CTAG 1 cut(s) 89
GlaI GCGC 1 cut(s) 315
GluI GCNGC 2 cut(s) 81, 84
GsuI CTGGAG 2 cut(s) 253, 417
HaeII RGCGCY 1 cut(s) 317
HhaI GCGC 1 cut(s) 316
Hin1II CATG 2 cut(s) 129, 520
Hin6I GCGC 1 cut(s) 314
HinP1I GCGC 1 cut(s) 314
HincII GTYRAC 1 cut(s) 529
HindII GTYRAC 1 cut(s) 529
HindIII AAGCTT 1 cut(s) 188
HinfI GANTC 1 cut(s) 255
Hpy166II GTNNAC 1 cut(s) 529
Hpy188III TCNNGA 1 cut(s) 439
Hpy8I GTNNAC 1 cut(s) 529
HpyAV CCTTC 2 cut(s) 320, 357
HpyCH4III ACNGT 3 cut(s) 371, 388, 551
HpyCH4IV ACGT 1 cut(s) 357
HpyCH4V TGCA 2 cut(s) 43, 299
HpyF10VI GCNNNNNNNGC 4 cut(s) 89, 183, 305, 407
HpyF3I CTNAG 2 cut(s) 145, 475
HpySE526I ACGT 1 cut(s) 357
Hsp92II CATG 2 cut(s) 129, 520
HspAI GCGC 1 cut(s) 314
Kzo9I GATC 1 cut(s) 71
LmnI GCTCC 2 cut(s) 398, 438
LpnPI CCDG 6 cut(s) 76, 163, 200, 217, 336, 381
Lsp1109I GCAGC 2 cut(s) 92, 95
LweI GCATC 1 cut(s) 286
MaeI CTAG 1 cut(s) 89
MaeII ACGT 1 cut(s) 357
MalI GATC 1 cut(s) 73
MboI GATC 1 cut(s) 71
MboII GAAGA 3 cut(s) 165, 168, 353
MhlI GDGCHC 4 cut(s) 202, 403, 423, 435
MluCI AATT 1 cut(s) 533
MlyI GAGTC 1 cut(s) 264
MnlI CCTC 5 cut(s) 62, 216, 219, 222, 446
MseI TTAA 1 cut(s) 335
MwoI GCNNNNNNNGC 4 cut(s) 89, 183, 305, 407
NdeII GATC 1 cut(s) 71
NlaIII CATG 2 cut(s) 129, 520
PkrI GCNGC 2 cut(s) 82, 85
PleI GAGTC 1 cut(s) 263
PpsI GAGTC 1 cut(s) 263
PpuMI RGGWCCY 1 cut(s) 329
Psp124BI GAGCTC 2 cut(s) 403, 435
Psp1406I AACGTT 1 cut(s) 357
Psp5II RGGWCCY 1 cut(s) 329
PspPI GGNCC 2 cut(s) 320, 329
PspPPI RGGWCCY 1 cut(s) 329
RsaI GTAC 1 cut(s) 368
RsaNI GTAC 1 cut(s) 367
SacI GAGCTC 2 cut(s) 403, 435
SaqAI TTAA 1 cut(s) 335
SatI GCNGC 2 cut(s) 81, 84
Sau3AI GATC 1 cut(s) 71
Sau96I GGNCC 2 cut(s) 320, 329
SchI GAGTC 1 cut(s) 264
SduI GDGCHC 4 cut(s) 202, 403, 423, 435
SfaNI GCATC 1 cut(s) 286
SfcI CTRYAG 1 cut(s) 261
SinI GGWCC 2 cut(s) 320, 329
SmlI CTYRAG 3 cut(s) 103, 437, 501
SmoI CTYRAG 3 cut(s) 103, 437, 501
Sse9I AATT 1 cut(s) 533
SsiI CCGC 1 cut(s) 453
SspMI CTAG 1 cut(s) 89
SstI GAGCTC 2 cut(s) 403, 435
TaaI ACNGT 3 cut(s) 371, 388, 551
TaiI ACGT 1 cut(s) 360
TasI AATT 1 cut(s) 533
Tru1I TTAA 1 cut(s) 335
Tru9I TTAA 1 cut(s) 335
TscAI CASTG 2 cut(s) 236, 400
TseI GCWGC 2 cut(s) 80, 83
TspRI CASTG 2 cut(s) 236, 400
VpaK11BI GGWCC 2 cut(s) 320, 329
XapI RAATTY 1 cut(s) 533
XspI CTAG 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.