MD00G1057700.v1.1

Protein of unknown function (DUF751)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0015477
Physical Location & Seq
Reverse (-)
10613751 .. 10614383
633 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1057700.v1.1.491

Sequence Viewer

Length: 633 bp
ATGAAAATGTCTACACTGAGCTCCCAATTCCACCTCCTAAAATACCCCGTCAAATATCCTTCATATCCATCCTCAAACCCTAACTCATTTCTCATCCCCGTCTCCCTACCAAAACACCCTCAACATCCAACCATATTATCATCTCTCCCTTCCGAAGCCACAAGCAATAGTCCTCCACCAACAAGAGCATCTGCTACAAAATCCAAGTTATATAGTGTAACGCTCGTCCGGAAAGAGTCTGTCAACGATACTTCCACCCCAATAAACTCTAGTGGAAACAACGCGTCAAGGTTTTGGATAATGGGAGCTGTTTCCGTAGGAGTTATGGCGGCGCTGATGGCGATGGATGAGCAGAAGGCAATGGCATTGGGCCCCGAAGGGCCGCTGATGGAAGAGTTTTGGGACAATGTACGGAGATATGGACTGTATGCTCTCACGGTGAGTACAGGTGCTCTGTACGCGACCTTCGCGCCTCTATACGAGTTGTTGAAGAACCCTATCTCTGCAGTTCTCGTTTTGGTAATATTAGGGGGAGGTATTTTCATCTTCTCCCAAGTGCTTTATGCCATGGTTGGTGTCTCAGATTTTAGTTATGATTATGGATATATCAATCCATGGATTCCTTCCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF751

Protein Analysis

211

Amino Acids

22.93

Weight (kDa)

8.71

Isoelectric Point (pI)

43.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF751 PF05421 133 - 192 6.3e-21 Protein of unknown function (DUF751)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015477)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16410
fragaria_vesca FvH4_3g00530
malus_domestica MD00G1057700.v1.1 MD10G1337000.v1.1
prunus_persica Prupe.4G006400_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0000731
rosa_laevigata RLG00000030890
rosa_multiflora Rmu_sc0000255.1_g000003 Rmu_sc0011992.1_g000006
rosa_roxburghii Rroxscaffold_1G00075500
rosa_rugosa Rorug04G0386100
rosa_samantha Rh5AG006000 Rh5BG007800 Rh5CG006500 Rh5DG006500
rosa_wichuraiana Rw5G000610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 11
AccII CGCG 3 cut(s) 284, 461, 470
AccIII TCCGGA 1 cut(s) 228
AciI CCGC 2 cut(s) 329, 383
AfaI GTAC 3 cut(s) 411, 445, 458
AflIII ACRYGT 1 cut(s) 282
AgsI TTSAA 1 cut(s) 490
AluBI AGCT 2 cut(s) 21, 308
AluI AGCT 2 cut(s) 21, 308
Alw21I GWGCWC 2 cut(s) 23, 454
Alw26I GTCTC 2 cut(s) 106, 583
Aor13HI TCCGGA 1 cut(s) 228
AoxI GGCC 2 cut(s) 370, 380
ApaI GGGCCC 1 cut(s) 374
AspLEI GCGC 2 cut(s) 334, 472
AspS9I GGNCC 3 cut(s) 370, 371, 380
AsuHPI GGTGA 1 cut(s) 451
BaeGI GKGCMC 1 cut(s) 374
BanII GRGCYC 2 cut(s) 23, 374
BarI GAAGNNNNNNTAC 2 cut(s) 449, 481
Bbv12I GWGCWC 2 cut(s) 23, 454
BccI CCATC 4 cut(s) 76, 331, 337, 382
BcoDI GTCTC 2 cut(s) 106, 583
BfaI CTAG 1 cut(s) 270
BfmI CTRYAG 1 cut(s) 504
BfoI RGCGCY 1 cut(s) 335
BisI GCNGC 2 cut(s) 330, 383
BlsI GCNGC 2 cut(s) 331, 384
BmgT120I GGNCC 3 cut(s) 370, 371, 380
BmiI GGNNCC 2 cut(s) 372, 373
BmsI GCATC 1 cut(s) 197
BsaJI CCNNGG 2 cut(s) 567, 614
BsaWI WCCGGW 1 cut(s) 228
Bse3DI GCAATG 1 cut(s) 366
BseAI TCCGGA 1 cut(s) 228
BseDI CCNNGG 2 cut(s) 567, 614
BseGI GGATG 4 cut(s) 68, 93, 124, 352
BseMI GCAATG 1 cut(s) 366
BseMII CTCAG 2 cut(s) 8, 594
BseSI GKGCMC 1 cut(s) 374
Bsh1236I CGCG 3 cut(s) 284, 461, 470
BshFI GGCC 2 cut(s) 372, 382
BsiHKAI GWGCWC 2 cut(s) 23, 454
BsiSI CCGG 1 cut(s) 229
BslFI GGGAC 1 cut(s) 416
BsmAI GTCTC 2 cut(s) 106, 583
BsmBI CGTCTC 1 cut(s) 106
BsmFI GGGAC 1 cut(s) 416
BsnI GGCC 2 cut(s) 372, 382
Bsp120I GGGCCC 1 cut(s) 370
Bsp1286I GDGCHC 3 cut(s) 23, 374, 454
Bsp13I TCCGGA 1 cut(s) 228
Bsp19I CCATGG 2 cut(s) 567, 614
BspACI CCGC 2 cut(s) 329, 383
BspANI GGCC 2 cut(s) 372, 382
BspCNI CTCAG 2 cut(s) 9, 593
BspEI TCCGGA 1 cut(s) 228
BspFNI CGCG 3 cut(s) 284, 461, 470
BspLI GGNNCC 2 cut(s) 372, 373
BspMAI CTGCAG 1 cut(s) 508
BsrDI GCAATG 1 cut(s) 366
BssECI CCNNGG 2 cut(s) 567, 614
BssT1I CCWWGG 2 cut(s) 567, 614
Bst4CI ACNGT 2 cut(s) 426, 439
Bst6I CTCTTC 1 cut(s) 387
BstDEI CTNAG 2 cut(s) 17, 580
BstDSI CCRYGG 2 cut(s) 567, 614
BstF5I GGATG 4 cut(s) 68, 93, 124, 352
BstFNI CGCG 3 cut(s) 284, 461, 470
BstH2I RGCGCY 1 cut(s) 335
BstHHI GCGC 2 cut(s) 334, 472
BstMAI GTCTC 2 cut(s) 106, 583
BstMWI GCNNNNNNNGC 3 cut(s) 338, 458, 467
BstSFI CTRYAG 1 cut(s) 504
BstSLI GKGCMC 1 cut(s) 374
BstUI CGCG 3 cut(s) 284, 461, 470
BsuRI GGCC 2 cut(s) 372, 382
BtgI CCRYGG 2 cut(s) 567, 614
BtgZI GCGATG 1 cut(s) 356
BtsCI GGATG 4 cut(s) 68, 93, 124, 352
BtsIMutI CAGTG 1 cut(s) 14
CfoI GCGC 2 cut(s) 334, 472
Cfr13I GGNCC 3 cut(s) 370, 371, 380
CseI GACGC 1 cut(s) 273
Csp6I GTAC 3 cut(s) 410, 444, 457
CviAII CATG 3 cut(s) 568, 615, 628
CviJI RGCY 5 cut(s) 21, 158, 308, 372, 382
CviKI_1 RGCY 5 cut(s) 21, 158, 308, 372, 382
CviQI GTAC 3 cut(s) 410, 444, 457
DdeI CTNAG 2 cut(s) 17, 580
Eam1104I CTCTTC 1 cut(s) 387
EarI CTCTTC 1 cut(s) 387
Ecl136II GAGCTC 1 cut(s) 21
Eco130I CCWWGG 2 cut(s) 567, 614
Eco24I GRGCYC 2 cut(s) 23, 374
Eco53kI GAGCTC 1 cut(s) 21
EcoICRI GAGCTC 1 cut(s) 21
EcoO109I RGGNCCY 1 cut(s) 371
EcoT14I CCWWGG 2 cut(s) 567, 614
EcoT38I GRGCYC 2 cut(s) 23, 374
ErhI CCWWGG 2 cut(s) 567, 614
Esp3I CGTCTC 1 cut(s) 106
FaeI CATG 3 cut(s) 571, 618, 631
FaqI GGGAC 1 cut(s) 416
FatI CATG 3 cut(s) 567, 614, 627
FblI GTMKAC 1 cut(s) 11
Fnu4HI GCNGC 2 cut(s) 330, 383
FokI GGATG 4 cut(s) 55, 80, 111, 359
FriOI GRGCYC 2 cut(s) 23, 374
Fsp4HI GCNGC 2 cut(s) 330, 383
FspBI CTAG 1 cut(s) 270
GlaI GCGC 2 cut(s) 333, 471
GluI GCNGC 2 cut(s) 330, 383
HaeII RGCGCY 1 cut(s) 335
HaeIII GGCC 2 cut(s) 372, 382
HapII CCGG 1 cut(s) 229
HgaI GACGC 1 cut(s) 273
HhaI GCGC 2 cut(s) 334, 472
Hin1II CATG 3 cut(s) 571, 618, 631
Hin6I GCGC 2 cut(s) 332, 470
HinP1I GCGC 2 cut(s) 332, 470
HincII GTYRAC 1 cut(s) 244
HindII GTYRAC 1 cut(s) 244
HinfI GANTC 2 cut(s) 236, 619
HpaII CCGG 1 cut(s) 229
HphI GGTGA 1 cut(s) 451
Hpy166II GTNNAC 2 cut(s) 12, 244
Hpy188I TCNGA 2 cut(s) 154, 583
Hpy188III TCNNGA 1 cut(s) 229
Hpy8I GTNNAC 2 cut(s) 12, 244
HpyAV CCTTC 6 cut(s) 69, 159, 349, 371, 475, 633
HpyCH4III ACNGT 2 cut(s) 426, 439
HpyCH4V TGCA 1 cut(s) 506
HpyF10VI GCNNNNNNNGC 3 cut(s) 338, 458, 467
HpyF3I CTNAG 2 cut(s) 17, 580
Hsp92II CATG 3 cut(s) 571, 618, 631
HspAI GCGC 2 cut(s) 332, 470
Kpn2I TCCGGA 1 cut(s) 228
LmnI GCTCC 2 cut(s) 26, 305
LpnPI CCDG 2 cut(s) 242, 432
LweI GCATC 1 cut(s) 197
MaeI CTAG 1 cut(s) 270
MaeIII GTNAC 1 cut(s) 217
MboII GAAGA 3 cut(s) 404, 502, 538
MhlI GDGCHC 3 cut(s) 23, 374, 454
MluCI AATT 1 cut(s) 26
MluI ACGCGT 1 cut(s) 282
MlyI GAGTC 1 cut(s) 245
MmeI TCCRAC 1 cut(s) 152
MnlI CCTC 6 cut(s) 44, 82, 129, 183, 483, 527
MroI TCCGGA 1 cut(s) 228
MspA1I CMGCKG 1 cut(s) 385
MspI CCGG 1 cut(s) 229
MvnI CGCG 3 cut(s) 284, 461, 470
MwoI GCNNNNNNNGC 3 cut(s) 338, 458, 467
NcoI CCATGG 2 cut(s) 567, 614
NlaIII CATG 3 cut(s) 571, 618, 631
NlaIV GGNNCC 2 cut(s) 372, 373
PfeI GAWTC 1 cut(s) 619
PkrI GCNGC 2 cut(s) 331, 384
PleI GAGTC 1 cut(s) 244
PpsI GAGTC 1 cut(s) 244
Psp124BI GAGCTC 1 cut(s) 23
PspN4I GGNNCC 2 cut(s) 372, 373
PspOMI GGGCCC 1 cut(s) 370
PspPI GGNCC 3 cut(s) 370, 371, 380
PstI CTGCAG 1 cut(s) 508
RsaI GTAC 3 cut(s) 411, 445, 458
RsaNI GTAC 3 cut(s) 410, 444, 457
SacI GAGCTC 1 cut(s) 23
SatI GCNGC 2 cut(s) 330, 383
Sau96I GGNCC 3 cut(s) 370, 371, 380
SchI GAGTC 1 cut(s) 245
SduI GDGCHC 3 cut(s) 23, 374, 454
SetI ASST 7 cut(s) 23, 36, 293, 310, 451, 467, 538
SfaNI GCATC 1 cut(s) 197
SfcI CTRYAG 1 cut(s) 504
Sse9I AATT 1 cut(s) 26
SsiI CCGC 2 cut(s) 329, 383
SspI AATATT 1 cut(s) 525
SspMI CTAG 1 cut(s) 270
SstI GAGCTC 1 cut(s) 23
StyI CCWWGG 2 cut(s) 567, 614
TaaI ACNGT 2 cut(s) 426, 439
TasI AATT 1 cut(s) 26
TatI WGTACW 1 cut(s) 443
TauI GCSGC 2 cut(s) 332, 385
TfiI GAWTC 1 cut(s) 619
TscAI CASTG 1 cut(s) 21
TspDTI ATGAA 3 cut(s) 17, 51, 532
TspGWI ACGGA 2 cut(s) 304, 427
TspRI CASTG 1 cut(s) 21
XmiI GTMKAC 1 cut(s) 11
XspI CTAG 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.