AT4G18950

Ankyrin repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0010746
Physical Location & Seq
Forward (+)
10375244 .. 10378416
3173 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G18950.1

Sequence Viewer

Length: 1380 bp
ATGGAAGAGGATTATCAACAGCCGAGGTTTACGATTGGTAGGCAATCATCAATGGCGCCGGAGAAGATTCCGGAGCCGTCGGTTCACTCAGAAGAAGAGGTGTTTGAGGATGGAGAAGAGATCGATGGTGGTGTGAGACTAATGTATTTGGCTAATGAAGGTGACATTGAAGGGATTAAGGAGCTTATTGATTCAGGGATTGATGCTAATTACAGAGACATTGATGATCGGACTGCTTTACACGTGGCGGCTTGTCAAGGATTGAAAGATGTTGTTGAGCTTCTTCTTGATCGGAAAGCTGAGGTTGATCCTAAAGATCGTTGGGGAAGCACTCCATTTGCAGATGCGATATTTTACAAGAACATTGATGTTATCAAGATTCTTGAGATACATGGAGCTAAACATCCGATGGCTCCAATGCACGTTAAGACTGCTCGTGAAGTCCCTGAGTATGAAATAAATCCTAGTGAGCTTGATTTCACTCAAAGCAAAGAGATAACAAAGGGAACTTACTGTATGGCAATGTGGCGTGGTATTCAAGTTGCGGTGAAAAAGCTGGATGATGAAGTTTTGAGCGATGACGATCAAGTGAGGAAGTTCCATGATGAGCTTGCATTGCTCCAAAGGCTTAGGCATCCAAACATTGTGCAGTTTCTTGGTGCTGTAACCCAAAGTAACCCAATGATGATTGTGACTGAATATTTGCCCAGGGGGGATTTGCGTGAATTGCTCAAACGAAAAGGACAATTGAAACCAGCTACTGCTGTTAGATATGCCCTTGATATTGCTAGGGGAATGAGCTATCTTCATGAGATCAAAGGAGACCCTATAATCCACCGCGATCTTGAACCTTCAAACATTCTGCGGGATGATTCAGGGCATCTGAAAGTTGCAGACTTTGGAGTAAGCAAGCTTGTTACTGTTAAAGAAGACAAGCCTTTTACATGTCAAGACATTTCTTGTCGATATATAGCTCCCGAGGTTTTCACTAGTGAAGAATACGATACAAAAGCTGATGTTTTCTCATTTGCATTGATCGTTCAGGAGATGATCGAAGGCCGAATGCCGTTTGCTGAAAAGGAAGACAGTGAAGCTTCTGAAGCTTATGCTGGCAAACATCGGCCATTATTCAAAGCTCCATCAAAGAATTACCCACATGGTCTTAAAACGTTGATAGAAGAATGCTGGCATGAGAAACCTGCAAAGCGACCAACTTTCAGAGAGATCATTAAACGACTTGAGTCCATTCTTCACCACATGGGTCACAAGCGACAATGGAGGATGAGGCCATTGACATGCTTTCAGAATTTCGAGCACAAGAAGAAACATAATTGGGATTTGAGCAGCCATGACGGCTCATCATCCGGTTCACATTTGTGA

Protein Analysis

459

Amino Acids

52.62

Weight (kDa)

5.71

Isoelectric Point (pI)

52.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 47 - 133 9.2e-14 Ankyrin repeats (3 copies)
Ank_4 PF13637 47 - 96 4.5e-07 Ankyrin repeats (many copies)
Ank PF00023 75 - 106 5.8e-06 Ankyrin repeat
PK_Tyr_Ser-Thr PF07714 165 - 413 4.3e-57 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 172 - 411 3e-52 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010746)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18950 AT4G18950
fragaria_vesca FvH4_3g06740 FvH4_3g06740 FvH4_3g06740 FvH4_3g06740 FvH4_3g06740 FvH4_3g06740
malus_domestica MD05G1293800.v1.1 MD10G1271100.v1.1
prunus_persica Prupe.4G070400_v2.0.a1
pyrus_communis pycom05g27110 pycom10g22690
rosa_chinensis RchiOBHm_Chr5g0012421
rosa_laevigata RLG00000031912
rosa_multiflora Rmu_sc0003469.1_g000008
rosa_roxburghii Rroxscaffold_1G00064250
rosa_rugosa Rorug05G0000900
rosa_samantha Rh5BG091700
rosa_wichuraiana Rw5G008350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1207
AccB1I GGYRCC 1 cut(s) 55
AccII CGCG 1 cut(s) 840
AccIII TCCGGA 1 cut(s) 70
AciI CCGC 4 cut(s) 248, 545, 838, 865
AclI AACGTT 1 cut(s) 1169
AclWI GGATC 1 cut(s) 302
AcoI YGGCCR 1 cut(s) 1121
AcsI RAATTY 1 cut(s) 1306
AcuI CTGAAG 1 cut(s) 1119
AcvI CACGTG 1 cut(s) 244
AcyI GRCGYC 1 cut(s) 56
AflIII ACRYGT 2 cut(s) 241, 944
AgsI TTSAA 7 cut(s) 170, 265, 539, 751, 848, 855, 1132
AhlI ACTAGT 1 cut(s) 989
AjnI CCWGG 1 cut(s) 707
AleI CACNNNNGTG 1 cut(s) 1375
Alw21I GWGCWC 1 cut(s) 1317
Alw26I GTCTC 3 cut(s) 130, 210, 816
AlwI GGATC 1 cut(s) 302
AlwNI CAGNNNCTG 1 cut(s) 761
Ama87I CYCGRG 1 cut(s) 977
Aor13HI TCCGGA 1 cut(s) 70
AoxI GGCC 3 cut(s) 1057, 1121, 1286
ApeKI GCWGC 1 cut(s) 1344
ApoI RAATTY 1 cut(s) 1306
ArsI GACNNNNNNTTYG 2 cut(s) 685, 717
AspLEI GCGC 1 cut(s) 58
AsuHPI GGTGA 3 cut(s) 173, 559, 1244
AvaI CYCGRG 1 cut(s) 977
BanI GGYRCC 1 cut(s) 55
BauI CACGAG 1 cut(s) 435
BbrPI CACGTG 1 cut(s) 244
BbsI GAAGAC 2 cut(s) 936, 1089
Bbv12I GWGCWC 1 cut(s) 1317
BbvCI CCTCAGC 1 cut(s) 300
BbvI GCAGC 1 cut(s) 1356
BccI CCATC 4 cut(s) 104, 119, 403, 1147
BceAI ACGGC 3 cut(s) 61, 1051, 1369
BciT130I CCWGG 1 cut(s) 709
BcoDI GTCTC 3 cut(s) 130, 210, 816
BcuI ACTAGT 1 cut(s) 989
BfaI CTAG 3 cut(s) 465, 789, 990
BfoI RGCGCY 1 cut(s) 59
BfuAI ACCTGC 1 cut(s) 1207
BglI GCCNNNNNGGC 1 cut(s) 1353
BisI GCNGC 2 cut(s) 249, 1345
BlsI GCNGC 2 cut(s) 250, 1346
Bme1390I CCNGG 1 cut(s) 709
BmeT110I CYCGRG 1 cut(s) 977
BmiI GGNNCC 3 cut(s) 57, 75, 414
BmrFI CCNGG 1 cut(s) 709
BmsI GCATC 4 cut(s) 193, 334, 643, 889
BoxI GACNNNNGTC 1 cut(s) 1240
BpiI GAAGAC 2 cut(s) 936, 1089
Bpu10I CCTNAGC 2 cut(s) 300, 629
BpuEI CTTGAG 2 cut(s) 404, 1259
Bsa29I ATCGAT 1 cut(s) 123
BsaAI YACGTR 1 cut(s) 244
BsaBI GATNNNNATC 1 cut(s) 582
BsaHI GRCGYC 1 cut(s) 56
BsaI GGTCTC 1 cut(s) 816
BsaJI CCNNGG 4 cut(s) 23, 707, 708, 978
BsaWI WCCGGW 2 cut(s) 70, 1364
Bse3DI GCAATG 2 cut(s) 528, 614
Bse8I GATNNNNATC 1 cut(s) 582
BseAI TCCGGA 1 cut(s) 70
BseBI CCWGG 1 cut(s) 709
BseCI ATCGAT 1 cut(s) 123
BseDI CCNNGG 4 cut(s) 23, 707, 708, 978
BseGI GGATG 7 cut(s) 115, 403, 565, 634, 874, 1287, 1361
BseJI GATNNNNATC 1 cut(s) 582
BseMI GCAATG 2 cut(s) 528, 614
BseMII CTCAG 3 cut(s) 102, 291, 438
BseXI GCAGC 1 cut(s) 1356
BsgI GTGCAG 1 cut(s) 668
Bsh1236I CGCG 1 cut(s) 840
BshFI GGCC 3 cut(s) 1059, 1123, 1288
BshNI GGYRCC 1 cut(s) 55
BshVI ATCGAT 1 cut(s) 123
BsiHKAI GWGCWC 1 cut(s) 1317
BsiHKCI CYCGRG 1 cut(s) 977
BsiSI CCGG 3 cut(s) 59, 71, 1365
BslFI GGGAC 1 cut(s) 428
BsmAI GTCTC 3 cut(s) 130, 210, 816
BsmFI GGGAC 1 cut(s) 428
BsmI GAATGC 2 cut(s) 1068, 1187
BsnI GGCC 3 cut(s) 1059, 1123, 1288
Bso31I GGTCTC 1 cut(s) 816
BsoBI CYCGRG 1 cut(s) 977
Bsp1286I GDGCHC 1 cut(s) 1317
Bsp13I TCCGGA 1 cut(s) 70
BspACI CCGC 4 cut(s) 248, 545, 838, 865
BspANI GGCC 3 cut(s) 1059, 1123, 1288
BspCNI CTCAG 3 cut(s) 101, 292, 439
BspDI ATCGAT 1 cut(s) 123
BspEI TCCGGA 1 cut(s) 70
BspFNI CGCG 1 cut(s) 840
BspHI TCATGA 1 cut(s) 808
BspLI GGNNCC 3 cut(s) 57, 75, 414
BspMI ACCTGC 1 cut(s) 1207
BspPI GGATC 1 cut(s) 302
BspT107I GGYRCC 1 cut(s) 55
BspTNI GGTCTC 1 cut(s) 816
BsrDI GCAATG 2 cut(s) 528, 614
BssECI CCNNGG 4 cut(s) 23, 707, 708, 978
BssNI GRCGYC 1 cut(s) 56
BssSI CACGAG 1 cut(s) 435
Bst2BI CACGAG 1 cut(s) 435
Bst2UI CCWGG 1 cut(s) 709
Bst4CI ACNGT 3 cut(s) 515, 922, 1088
Bst6I CTCTTC 2 cut(s) 90, 111
BstACI GRCGYC 1 cut(s) 56
BstBAI YACGTR 1 cut(s) 244
BstC8I GCNNGC 4 cut(s) 612, 911, 1111, 1187
BstDEI CTNAG 4 cut(s) 88, 300, 447, 629
BstF5I GGATG 7 cut(s) 115, 403, 565, 634, 874, 1287, 1361
BstFNI CGCG 1 cut(s) 840
BstH2I RGCGCY 1 cut(s) 59
BstHHI GCGC 1 cut(s) 58
BstMAI GTCTC 3 cut(s) 130, 210, 816
BstMWI GCNNNNNNNGC 5 cut(s) 616, 625, 727, 1100, 1353
BstNI CCWGG 1 cut(s) 709
BstNSI RCATGY 2 cut(s) 948, 1299
BstPAI GACNNNNGTC 1 cut(s) 1240
BstSCI CCNGG 1 cut(s) 707
BstUI CGCG 1 cut(s) 840
BstV1I GCAGC 1 cut(s) 1356
BstV2I GAAGAC 2 cut(s) 936, 1089
Bsu15I ATCGAT 1 cut(s) 123
BsuRI GGCC 3 cut(s) 1059, 1123, 1288
BsuTUI ATCGAT 1 cut(s) 123
BtgZI GCGATG 1 cut(s) 591
BtsCI GGATG 7 cut(s) 115, 403, 565, 634, 874, 1287, 1361
BtsIMutI CAGTG 1 cut(s) 1093
BveI ACCTGC 1 cut(s) 1207
Cac8I GCNNGC 4 cut(s) 612, 911, 1111, 1187
CaiI CAGNNNCTG 1 cut(s) 761
CciI TCATGA 1 cut(s) 808
CfoI GCGC 1 cut(s) 58
ClaI ATCGAT 1 cut(s) 123
CviAII CATG 9 cut(s) 392, 602, 809, 945, 1157, 1190, 1258, 1296, 1349
DdeI CTNAG 4 cut(s) 88, 300, 447, 629
DinI GGCGCC 1 cut(s) 57
EaeI YGGCCR 1 cut(s) 1121
Eam1104I CTCTTC 2 cut(s) 90, 111
EarI CTCTTC 2 cut(s) 90, 111
Eco31I GGTCTC 1 cut(s) 816
Eco57I CTGAAG 1 cut(s) 1119
Eco72I CACGTG 1 cut(s) 244
Eco88I CYCGRG 1 cut(s) 977
EcoRII CCWGG 1 cut(s) 707
EgeI GGCGCC 1 cut(s) 57
EheI GGCGCC 1 cut(s) 57
FaeI CATG 9 cut(s) 395, 605, 812, 948, 1160, 1193, 1261, 1299, 1352
FaqI GGGAC 1 cut(s) 428
FatI CATG 9 cut(s) 391, 601, 808, 944, 1156, 1189, 1257, 1295, 1348
FauI CCCGC 1 cut(s) 858
Fnu4HI GCNGC 2 cut(s) 249, 1345
FokI GGATG 7 cut(s) 122, 390, 572, 621, 881, 1294, 1348
Fsp4HI GCNGC 2 cut(s) 249, 1345
FspBI CTAG 3 cut(s) 465, 789, 990
GlaI GCGC 1 cut(s) 57
GluI GCNGC 2 cut(s) 249, 1345
HaeII RGCGCY 1 cut(s) 59
HaeIII GGCC 3 cut(s) 1059, 1123, 1288
HapII CCGG 3 cut(s) 59, 71, 1365
HhaI GCGC 1 cut(s) 58
Hin1I GRCGYC 1 cut(s) 56
Hin1II CATG 9 cut(s) 395, 605, 812, 948, 1160, 1193, 1261, 1299, 1352
Hin6I GCGC 1 cut(s) 56
HinP1I GCGC 1 cut(s) 56
HindIII AAGCTT 3 cut(s) 911, 1092, 1101
HinfI GANTC 5 cut(s) 67, 191, 379, 872, 1241
HpaII CCGG 3 cut(s) 59, 71, 1365
HphI GGTGA 3 cut(s) 173, 559, 1244
Hpy166II GTNNAC 3 cut(s) 30, 85, 1370
Hpy188I TCNGA 8 cut(s) 91, 231, 294, 408, 885, 1099, 1220, 1305
Hpy8I GTNNAC 3 cut(s) 30, 85, 1370
Hpy99I CGWCG 1 cut(s) 82
HpyAV CCTTC 4 cut(s) 152, 164, 861, 1049
HpyCH4III ACNGT 3 cut(s) 515, 922, 1088
HpyCH4IV ACGT 3 cut(s) 243, 423, 1169
HpyCH4V TGCA 7 cut(s) 341, 421, 614, 649, 893, 1031, 1202
HpyF10VI GCNNNNNNNGC 5 cut(s) 616, 625, 727, 1100, 1353
HpyF3I CTNAG 4 cut(s) 88, 300, 447, 629
HpySE526I ACGT 3 cut(s) 243, 423, 1169
Hsp92I GRCGYC 1 cut(s) 56
Hsp92II CATG 9 cut(s) 395, 605, 812, 948, 1160, 1193, 1261, 1299, 1352
HspAI GCGC 1 cut(s) 56
KasI GGCGCC 1 cut(s) 55
Kpn2I TCCGGA 1 cut(s) 70
LmnI GCTCC 7 cut(s) 73, 181, 395, 418, 624, 979, 1141
Lsp1109I GCAGC 1 cut(s) 1356
LweI GCATC 4 cut(s) 193, 334, 643, 889
MaeI CTAG 3 cut(s) 465, 789, 990
MaeII ACGT 3 cut(s) 243, 423, 1169
MaeIII GTNAC 6 cut(s) 161, 664, 674, 691, 916, 1262
MfeI CAATTG 1 cut(s) 746
MhlI GDGCHC 1 cut(s) 1317
MluCI AATT 6 cut(s) 208, 725, 746, 1147, 1306, 1330
Mly113I GGCGCC 1 cut(s) 56
MlyI GAGTC 1 cut(s) 1250
MnlI CCTC 8 cut(s) 18, 91, 100, 295, 585, 973, 1272, 1278
MroI TCCGGA 1 cut(s) 70
MseI TTAA 5 cut(s) 177, 426, 924, 1164, 1230
MslI CAYNNNNRTG 2 cut(s) 1294, 1375
MspI CCGG 3 cut(s) 59, 71, 1365
MspR9I CCNGG 1 cut(s) 709
MunI CAATTG 1 cut(s) 746
Mva1269I GAATGC 2 cut(s) 1068, 1187
MvaI CCWGG 1 cut(s) 709
MvnI CGCG 1 cut(s) 840
MwoI GCNNNNNNNGC 5 cut(s) 616, 625, 727, 1100, 1353
NarI GGCGCC 1 cut(s) 56
NlaIII CATG 9 cut(s) 395, 605, 812, 948, 1160, 1193, 1261, 1299, 1352
NlaIV GGNNCC 3 cut(s) 57, 75, 414
NmeAIII GCCGAG 1 cut(s) 48
NmuCI GTSAC 3 cut(s) 161, 691, 1262
NspI RCATGY 2 cut(s) 948, 1299
OliI CACNNNNGTG 1 cut(s) 1375
PagI TCATGA 1 cut(s) 808
PasI CCCWGGG 1 cut(s) 708
PciI ACATGT 1 cut(s) 944
PctI GAATGC 2 cut(s) 1068, 1187
PfeI GAWTC 4 cut(s) 67, 191, 379, 872
PkrI GCNGC 2 cut(s) 250, 1346
PleI GAGTC 1 cut(s) 1249
PluTI GGCGCC 1 cut(s) 59
PmaCI CACGTG 1 cut(s) 244
PmlI CACGTG 1 cut(s) 244
PpsI GAGTC 1 cut(s) 1249
Ppu21I YACGTR 1 cut(s) 244
PscI ACATGT 1 cut(s) 944
PshAI GACNNNNGTC 1 cut(s) 1240
Psp1406I AACGTT 1 cut(s) 1169
Psp6I CCWGG 1 cut(s) 707
PspCI CACGTG 1 cut(s) 244
PspGI CCWGG 1 cut(s) 707
PspN4I GGNNCC 3 cut(s) 57, 75, 414
PstNI CAGNNNCTG 1 cut(s) 761
RseI CAYNNNNRTG 2 cut(s) 1294, 1375
SaqAI TTAA 5 cut(s) 177, 426, 924, 1164, 1230
SatI GCNGC 2 cut(s) 249, 1345
SchI GAGTC 1 cut(s) 1250
ScrFI CCNGG 1 cut(s) 709
SduI GDGCHC 1 cut(s) 1317
SfaNI GCATC 4 cut(s) 193, 334, 643, 889
SfoI GGCGCC 1 cut(s) 57
SmiMI CAYNNNNRTG 2 cut(s) 1294, 1375
SmlI CTYRAG 2 cut(s) 383, 1238
SmoI CTYRAG 2 cut(s) 383, 1238
SpeI ACTAGT 1 cut(s) 989
Sse9I AATT 6 cut(s) 208, 725, 746, 1147, 1306, 1330
SsiI CCGC 4 cut(s) 248, 545, 838, 865
SspDI GGCGCC 1 cut(s) 55
SspI AATATT 1 cut(s) 701
SspMI CTAG 3 cut(s) 465, 789, 990
StyD4I CCNGG 1 cut(s) 707
TaaI ACNGT 3 cut(s) 515, 922, 1088
TaiI ACGT 3 cut(s) 246, 426, 1172
TaqI TCGA 4 cut(s) 123, 964, 1053, 1311
TasI AATT 6 cut(s) 208, 725, 746, 1147, 1306, 1330
TauI GCSGC 1 cut(s) 251
TfiI GAWTC 4 cut(s) 67, 191, 379, 872
Tru1I TTAA 5 cut(s) 177, 426, 924, 1164, 1230
Tru9I TTAA 5 cut(s) 177, 426, 924, 1164, 1230
TscAI CASTG 1 cut(s) 1093
TseFI GTSAC 3 cut(s) 161, 691, 1262
TseI GCWGC 1 cut(s) 1344
Tsp45I GTSAC 3 cut(s) 161, 691, 1262
TspDTI ATGAA 4 cut(s) 171, 468, 579, 797
TspRI CASTG 1 cut(s) 1093
XapI RAATTY 1 cut(s) 1306
XceI RCATGY 2 cut(s) 948, 1299
XspI CTAG 3 cut(s) 465, 789, 990
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.