RLG00000031912

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0010746
Physical Location & Seq
Forward (+)
9413206 .. 9417962
4757 bp
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UTR
Exon/CDS
Intron
RLM00000031912

Sequence Viewer

Length: 1380 bp
ATGGATTTGAAAACGACGCCGTTGCGGTTCACTCTGGGCAAGCAGTCGTCTCTGGCGCCGGAGCGTGAATCCGAGGCCGCCGGAGATGCCGAGGAGGACGAAGTGGAGGTGATCGATCCCCGAGTGAGGCTCATGTACTTGGCCAATGAAGCCGACTTGGATGGGATCAAGGAGCTTCTCGATTCCGGCATCGACGTCAATTTCCGCGACATCGATAATCGGACGGCGCTGCATGTCGCCGCGTGTCAGGGCTACTCCGACGTCGTTGAGCTGCTGCTCGAGCGCGGAGCTGATGTTAATACTAAAGATCGCTGGGGCAGCTCCCCTCTTGCAGATGCTATATACTATAAGAACCATGATGTGATCAAGCTTCTGGAGAAGCGCGGCGCGAAGCCTCTGATGGCACCAATGCGCGTTAAGTATGCGAGAGAAGTTCCCGAATATGAAATTGATCCCAAGGAGCTTGATTTTACTAACAGTGTTGAAATAACCAAGGGAACATTCCATTTAGCATCTTGGCGTGGAATACAGGTTGCTGTGAAAAGGCTTGCGGAGGAACTAATTGTTGACGAGGCTAAAGTGAAGGCATTTACAGATGAGCTTGCATTGCTTCAAAAGATACGGCATCCAAATGTAGTGCAATTTCTGGGTGCAGTAACTCAAAGTAGTCCAATGATGATTGTAACAGAATACTTGCCTAAGGGAGACCTACGTGCATTTTTAAAAAGAAAAGGAGCATTGAAACCATCGACAGCTGTGAGATTTGCACTTGATATTGCAAGGGGAATGAATTATTTGCATGAGAATAAACCAGCACCAATTATTCATCGTGATCTTGAGCCTTCAAATATATTGCGAGATGATTCTGGGACCATAAAAGTTGCTGATTTTGGAGTGAGCAAGTTGCTGACAGTTAAAGAAGATAAGCCTTTGATTTGTCAAGATGCTTCATGTCGATACATAGCTCCTGAGGTTTCCAAGAATGAAGAATATGGTACAAAAGTGGATGTATTCTCTTTTGCTTTAATCCTACAAGAGATGATAGAAGGCTGTCCACCATTTTCTGCCAAGCAAGATAGTGAAGTTCCAAAAGTATATGTTGCACAAGAGCGCCCACCTTTCAGAGCTCCAGTAAAGCGTTATGCACATGGCCTTAGAGAGTTAATTGAGGAGTGCTGGAATGAGAAGCCAGCTAAAAGACCATCGTTTAGGCAAATAATCTTGAGGTTGGAATCTATTTATAACACCATCGGCCATAAAAGGCGTTGGAAGGTTAGACCATTGAAATGCTTTCCAAATCTAGAAGCCATGTTGAGGAAAGATCGTATCAGTCTGAGCAGCAGAAGCCGTTCATCACGTTCCTCTACTGGCAGCATATAA

Protein Analysis

460

Amino Acids

51.95

Weight (kDa)

8.33

Isoelectric Point (pI)

51.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 44 - 131 7.8e-16 Ankyrin repeats (3 copies)
Ank_4 PF13637 44 - 93 1.1e-06 Ankyrin repeats (many copies)
Ank_5 PF13857 59 - 110 2e-09 Ankyrin repeats (many copies)
Ank PF00023 72 - 104 3.1e-09 Ankyrin repeat
PK_Tyr_Ser-Thr PF07714 162 - 409 5.9e-55 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 173 - 407 1.3e-50 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010746)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18950 AT4G18950
fragaria_vesca FvH4_3g06740 FvH4_3g06740 FvH4_3g06740 FvH4_3g06740 FvH4_3g06740 FvH4_3g06740
malus_domestica MD05G1293800.v1.1 MD10G1271100.v1.1
prunus_persica Prupe.4G070400_v2.0.a1
pyrus_communis pycom05g27110 pycom10g22690
rosa_chinensis RchiOBHm_Chr5g0012421
rosa_laevigata RLG00000031912
rosa_multiflora Rmu_sc0003469.1_g000008
rosa_roxburghii Rroxscaffold_1G00064250
rosa_rugosa Rorug05G0000900
rosa_samantha Rh5BG091700
rosa_wichuraiana Rw5G008350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1242
AatII GACGTC 2 cut(s) 198, 264
AccB1I GGYRCC 2 cut(s) 55, 403
AccII CGCG 6 cut(s) 207, 242, 285, 384, 389, 414
AciI CCGC 7 cut(s) 25, 78, 205, 240, 285, 384, 551
AclWI GGATC 3 cut(s) 110, 173, 446
AcoI YGGCCR 2 cut(s) 141, 1252
AcyI GRCGYC 4 cut(s) 17, 56, 195, 261
AfaI GTAC 2 cut(s) 137, 997
AfiI CCNNNNNNNGG 2 cut(s) 126, 1314
AgsI TTSAA 6 cut(s) 10, 485, 614, 742, 846, 1285
AjuI GAANNNNNNNTTGG 2 cut(s) 485, 517
Alw21I GWGCWC 1 cut(s) 1129
Alw26I GTCTC 2 cut(s) 54, 699
AlwI GGATC 3 cut(s) 110, 173, 446
Ama87I CYCGRG 2 cut(s) 120, 278
AoxI GGCC 4 cut(s) 75, 141, 1150, 1252
ApeKI GCWGC 6 cut(s) 229, 271, 274, 318, 1338, 1371
Asp700I GAANNNNTTC 1 cut(s) 1348
AspLEI GCGC 7 cut(s) 58, 229, 285, 384, 389, 414, 1113
AspS9I GGNCC 1 cut(s) 870
AsuHPI GGTGA 1 cut(s) 121
AvaI CYCGRG 2 cut(s) 120, 278
AvaII GGWCC 1 cut(s) 870
AxyI CCTNAGG 2 cut(s) 699, 969
BaeI ACNNNNGTAYC 2 cut(s) 987, 1020
BalI TGGCCA 1 cut(s) 143
BanI GGYRCC 2 cut(s) 55, 403
BanII GRGCYC 1 cut(s) 1129
Bbv12I GWGCWC 1 cut(s) 1129
BbvI GCAGC 5 cut(s) 216, 258, 261, 330, 1350
BccI CCATC 5 cut(s) 155, 394, 754, 1210, 1256
BceAI ACGGC 4 cut(s) 4, 240, 638, 1332
BcgI CGANNNNNNTGC 2 cut(s) 4, 38
BclI TGATCA 1 cut(s) 363
BcoDI GTCTC 2 cut(s) 54, 699
BfaI CTAG 1 cut(s) 1301
BfoI RGCGCY 3 cut(s) 59, 230, 1114
BisI GCNGC 9 cut(s) 78, 230, 240, 272, 275, 319, 385, 1339, 1372
BlsI GCNGC 9 cut(s) 79, 231, 241, 273, 276, 320, 386, 1340, 1373
Bme18I GGWCC 1 cut(s) 870
BmeT110I CYCGRG 2 cut(s) 120, 278
BmgT120I GGNCC 1 cut(s) 870
BmiI GGNNCC 3 cut(s) 57, 405, 871
BmsI GCATC 6 cut(s) 76, 198, 325, 521, 634, 934
BplI GAGNNNNNCTC 2 cut(s) 114, 146
BpmI CTGGAG 2 cut(s) 395, 1113
BpuEI CTTGAG 2 cut(s) 857, 1243
Bsa29I ATCGAT 2 cut(s) 114, 213
BsaAI YACGTR 1 cut(s) 713
BsaHI GRCGYC 4 cut(s) 17, 56, 195, 261
BsaI GGTCTC 1 cut(s) 699
BsaJI CCNNGG 4 cut(s) 72, 90, 456, 492
BsaXI ACNNNNNCTCC 2 cut(s) 279, 309
Bsc4I CCNNNNNNNGG 2 cut(s) 126, 1314
Bse1I ACTGG 2 cut(s) 1130, 1372
Bse21I CCTNAGG 2 cut(s) 699, 969
Bse3DI GCAATG 1 cut(s) 605
BseCI ATCGAT 2 cut(s) 114, 213
BseDI CCNNGG 4 cut(s) 72, 90, 456, 492
BseGI GGATG 3 cut(s) 166, 625, 1012
BseLI CCNNNNNNNGG 2 cut(s) 126, 1314
BseMI GCAATG 1 cut(s) 605
BseMII CTCAG 2 cut(s) 960, 1325
BseNI ACTGG 2 cut(s) 1130, 1372
BseRI GAGGAG 2 cut(s) 107, 1184
BseXI GCAGC 5 cut(s) 216, 258, 261, 330, 1350
BseYI CCCAGC 1 cut(s) 312
BsgI GTGCAG 1 cut(s) 672
Bsh1236I CGCG 6 cut(s) 207, 242, 285, 384, 389, 414
BshFI GGCC 4 cut(s) 77, 143, 1152, 1254
BshNI GGYRCC 2 cut(s) 55, 403
BshVI ATCGAT 2 cut(s) 114, 213
BsiHKAI GWGCWC 1 cut(s) 1129
BsiHKCI CYCGRG 2 cut(s) 120, 278
BsiSI CCGG 3 cut(s) 59, 81, 186
BslFI GGGAC 1 cut(s) 883
BslI CCNNNNNNNGG 2 cut(s) 126, 1314
BsmAI GTCTC 2 cut(s) 54, 699
BsmBI CGTCTC 1 cut(s) 54
BsmFI GGGAC 1 cut(s) 883
BsnI GGCC 4 cut(s) 77, 143, 1152, 1254
Bso31I GGTCTC 1 cut(s) 699
BsoBI CYCGRG 2 cut(s) 120, 278
Bsp1286I GDGCHC 1 cut(s) 1129
Bsp143I GATC 8 cut(s) 111, 115, 165, 307, 363, 451, 832, 1321
BspACI CCGC 7 cut(s) 25, 78, 205, 240, 285, 384, 551
BspANI GGCC 4 cut(s) 77, 143, 1152, 1254
BspCNI CTCAG 2 cut(s) 961, 1326
BspDI ATCGAT 2 cut(s) 114, 213
BspFNI CGCG 6 cut(s) 207, 242, 285, 384, 389, 414
BspLI GGNNCC 3 cut(s) 57, 405, 871
BspPI GGATC 3 cut(s) 110, 173, 446
BspT107I GGYRCC 2 cut(s) 55, 403
BspTNI GGTCTC 1 cut(s) 699
BsrDI GCAATG 1 cut(s) 605
BsrI ACTGG 2 cut(s) 1130, 1372
BssECI CCNNGG 4 cut(s) 72, 90, 456, 492
BssMI GATC 8 cut(s) 111, 115, 165, 307, 363, 451, 832, 1321
BssNI GRCGYC 4 cut(s) 17, 56, 195, 261
BssT1I CCWWGG 2 cut(s) 456, 492
Bst4CI ACNGT 2 cut(s) 479, 913
BstACI GRCGYC 4 cut(s) 17, 56, 195, 261
BstBAI YACGTR 1 cut(s) 713
BstC8I GCNNGC 4 cut(s) 41, 549, 603, 1191
BstDEI CTNAG 4 cut(s) 699, 969, 1154, 1334
BstF5I GGATG 3 cut(s) 166, 625, 1012
BstFNI CGCG 6 cut(s) 207, 242, 285, 384, 389, 414
BstH2I RGCGCY 3 cut(s) 59, 230, 1114
BstHHI GCGC 7 cut(s) 58, 229, 285, 384, 389, 414, 1113
BstKTI GATC 8 cut(s) 114, 118, 168, 310, 366, 454, 835, 1324
BstMAI GTCTC 2 cut(s) 54, 699
BstMBI GATC 8 cut(s) 111, 115, 165, 307, 363, 451, 832, 1321
BstMWI GCNNNNNNNGC 6 cut(s) 86, 149, 280, 318, 607, 1344
BstNSI RCATGY 1 cut(s) 236
BstUI CGCG 6 cut(s) 207, 242, 285, 384, 389, 414
BstV1I GCAGC 5 cut(s) 216, 258, 261, 330, 1350
Bsu15I ATCGAT 2 cut(s) 114, 213
Bsu36I CCTNAGG 2 cut(s) 699, 969
BsuRI GGCC 4 cut(s) 77, 143, 1152, 1254
BsuTUI ATCGAT 2 cut(s) 114, 213
BtsCI GGATG 3 cut(s) 166, 625, 1012
BtsIMutI CAGTG 1 cut(s) 484
Cac8I GCNNGC 4 cut(s) 41, 549, 603, 1191
CfoI GCGC 7 cut(s) 58, 229, 285, 384, 389, 414, 1113
Cfr13I GGNCC 1 cut(s) 870
ClaI ATCGAT 2 cut(s) 114, 213
CseI GACGC 1 cut(s) 25
Csp6I GTAC 2 cut(s) 136, 996
CviAII CATG 7 cut(s) 133, 233, 356, 800, 951, 1148, 1309
CviQI GTAC 2 cut(s) 136, 996
DdeI CTNAG 4 cut(s) 699, 969, 1154, 1334
DinI GGCGCC 1 cut(s) 57
DpnI GATC 8 cut(s) 113, 117, 167, 309, 365, 453, 834, 1323
DpnII GATC 8 cut(s) 111, 115, 165, 307, 363, 451, 832, 1321
DraI TTTAAA 1 cut(s) 723
EaeI YGGCCR 2 cut(s) 141, 1252
Ecl136II GAGCTC 1 cut(s) 1127
Eco130I CCWWGG 2 cut(s) 456, 492
Eco24I GRGCYC 1 cut(s) 1129
Eco31I GGTCTC 1 cut(s) 699
Eco47I GGWCC 1 cut(s) 870
Eco53kI GAGCTC 1 cut(s) 1127
Eco81I CCTNAGG 2 cut(s) 699, 969
Eco88I CYCGRG 2 cut(s) 120, 278
EcoICRI GAGCTC 1 cut(s) 1127
EcoT14I CCWWGG 2 cut(s) 456, 492
EcoT38I GRGCYC 1 cut(s) 1129
EgeI GGCGCC 1 cut(s) 57
EheI GGCGCC 1 cut(s) 57
ErhI CCWWGG 2 cut(s) 456, 492
Esp3I CGTCTC 1 cut(s) 54
FaeI CATG 7 cut(s) 136, 236, 359, 803, 954, 1151, 1312
FaqI GGGAC 1 cut(s) 883
FatI CATG 7 cut(s) 132, 232, 355, 799, 950, 1147, 1308
FbaI TGATCA 1 cut(s) 363
Fnu4HI GCNGC 9 cut(s) 78, 230, 240, 272, 275, 319, 385, 1339, 1372
FokI GGATG 3 cut(s) 173, 612, 1019
FriOI GRGCYC 1 cut(s) 1129
Fsp4HI GCNGC 9 cut(s) 78, 230, 240, 272, 275, 319, 385, 1339, 1372
FspBI CTAG 1 cut(s) 1301
GlaI GCGC 7 cut(s) 57, 228, 284, 383, 388, 413, 1112
GluI GCNGC 9 cut(s) 78, 230, 240, 272, 275, 319, 385, 1339, 1372
GsaI CCCAGC 1 cut(s) 316
GsuI CTGGAG 2 cut(s) 395, 1113
HaeII RGCGCY 3 cut(s) 59, 230, 1114
HaeIII GGCC 4 cut(s) 77, 143, 1152, 1254
HapII CCGG 3 cut(s) 59, 81, 186
HgaI GACGC 1 cut(s) 25
HhaI GCGC 7 cut(s) 58, 229, 285, 384, 389, 414, 1113
Hin1I GRCGYC 4 cut(s) 17, 56, 195, 261
Hin1II CATG 7 cut(s) 136, 236, 359, 803, 954, 1151, 1312
Hin6I GCGC 7 cut(s) 56, 227, 283, 382, 387, 412, 1111
HinP1I GCGC 7 cut(s) 56, 227, 283, 382, 387, 412, 1111
HincII GTYRAC 1 cut(s) 568
HindII GTYRAC 1 cut(s) 568
HindIII AAGCTT 1 cut(s) 368
HinfI GANTC 4 cut(s) 68, 182, 863, 1232
HpaII CCGG 3 cut(s) 59, 81, 186
HphI GGTGA 1 cut(s) 121
Hpy166II GTNNAC 3 cut(s) 30, 568, 1055
Hpy188I TCNGA 6 cut(s) 73, 222, 259, 399, 1124, 1335
Hpy188III TCNNGA 9 cut(s) 179, 374, 437, 830, 836, 941, 968, 1222, 1301
Hpy8I GTNNAC 3 cut(s) 30, 568, 1055
Hpy99I CGWCG 4 cut(s) 19, 197, 263, 266
HpyAV CCTTC 4 cut(s) 577, 852, 1040, 1264
HpyCH4III ACNGT 2 cut(s) 479, 913
HpyCH4IV ACGT 4 cut(s) 195, 261, 712, 1357
HpyF10VI GCNNNNNNNGC 6 cut(s) 86, 149, 280, 318, 607, 1344
HpyF3I CTNAG 4 cut(s) 699, 969, 1154, 1334
HpySE526I ACGT 4 cut(s) 195, 261, 712, 1357
Hsp92I GRCGYC 4 cut(s) 17, 56, 195, 261
Hsp92II CATG 7 cut(s) 136, 236, 359, 803, 954, 1151, 1312
HspAI GCGC 7 cut(s) 56, 227, 283, 382, 387, 412, 1111
KasI GGCGCC 1 cut(s) 55
Ksp22I TGATCA 1 cut(s) 363
Kzo9I GATC 8 cut(s) 111, 115, 165, 307, 363, 451, 832, 1321
LmnI GCTCC 8 cut(s) 61, 172, 287, 326, 460, 734, 970, 1132
Lsp1109I GCAGC 5 cut(s) 216, 258, 261, 330, 1350
LweI GCATC 6 cut(s) 76, 198, 325, 521, 634, 934
MaeI CTAG 1 cut(s) 1301
MaeII ACGT 4 cut(s) 195, 261, 712, 1357
MaeIII GTNAC 2 cut(s) 655, 682
MalI GATC 8 cut(s) 113, 117, 167, 309, 365, 453, 834, 1323
MboI GATC 8 cut(s) 111, 115, 165, 307, 363, 451, 832, 1321
MboII GAAGA 2 cut(s) 932, 998
MhlI GDGCHC 1 cut(s) 1129
MlsI TGGCCA 1 cut(s) 143
MluCI AATT 7 cut(s) 199, 447, 561, 641, 790, 819, 1164
MluNI TGGCCA 1 cut(s) 143
Mly113I GGCGCC 1 cut(s) 56
MmeI TCCRAC 3 cut(s) 282, 1209, 1247
Mox20I TGGCCA 1 cut(s) 143
MroXI GAANNNNTTC 1 cut(s) 1348
MscI TGGCCA 1 cut(s) 143
MseI TTAA 6 cut(s) 297, 417, 722, 915, 1025, 1163
MslI CAYNNNNRTG 2 cut(s) 630, 1285
Msp20I TGGCCA 1 cut(s) 143
MspA1I CMGCKG 1 cut(s) 755
MspI CCGG 3 cut(s) 59, 81, 186
MteI GCGCNGCGC 1 cut(s) 385
MvnI CGCG 6 cut(s) 207, 242, 285, 384, 389, 414
MwoI GCNNNNNNNGC 6 cut(s) 86, 149, 280, 318, 607, 1344
NarI GGCGCC 1 cut(s) 56
NdeII GATC 8 cut(s) 111, 115, 165, 307, 363, 451, 832, 1321
NlaIII CATG 7 cut(s) 136, 236, 359, 803, 954, 1151, 1312
NlaIV GGNNCC 3 cut(s) 57, 405, 871
NmeAIII GCCGAG 1 cut(s) 115
NspI RCATGY 1 cut(s) 236
PaeR7I CTCGAG 1 cut(s) 278
PdmI GAANNNNTTC 1 cut(s) 1348
PfeI GAWTC 4 cut(s) 68, 182, 863, 1232
PkrI GCNGC 9 cut(s) 79, 231, 241, 273, 276, 320, 386, 1340, 1373
PluTI GGCGCC 1 cut(s) 59
Ppu21I YACGTR 1 cut(s) 713
PsiI TTATAA 1 cut(s) 1242
Psp124BI GAGCTC 1 cut(s) 1129
PspFI CCCAGC 1 cut(s) 312
PspN4I GGNNCC 3 cut(s) 57, 405, 871
PspPI GGNCC 1 cut(s) 870
PspXI VCTCGAGB 1 cut(s) 278
PvuII CAGCTG 1 cut(s) 755
RsaI GTAC 2 cut(s) 137, 997
RsaNI GTAC 2 cut(s) 136, 996
RseI CAYNNNNRTG 2 cut(s) 630, 1285
SacI GAGCTC 1 cut(s) 1129
SaqAI TTAA 6 cut(s) 297, 417, 722, 915, 1025, 1163
SatI GCNGC 9 cut(s) 78, 230, 240, 272, 275, 319, 385, 1339, 1372
Sau3AI GATC 8 cut(s) 111, 115, 165, 307, 363, 451, 832, 1321
Sau96I GGNCC 1 cut(s) 870
SduI GDGCHC 1 cut(s) 1129
SfaNI GCATC 6 cut(s) 76, 198, 325, 521, 634, 934
SfoI GGCGCC 1 cut(s) 57
Sfr274I CTCGAG 1 cut(s) 278
SinI GGWCC 1 cut(s) 870
SlaI CTCGAG 1 cut(s) 278
SmiMI CAYNNNNRTG 2 cut(s) 630, 1285
SmlI CTYRAG 3 cut(s) 278, 836, 1222
SmoI CTYRAG 3 cut(s) 278, 836, 1222
Sse9I AATT 7 cut(s) 199, 447, 561, 641, 790, 819, 1164
SsiI CCGC 7 cut(s) 25, 78, 205, 240, 285, 384, 551
SspDI GGCGCC 1 cut(s) 55
SspMI CTAG 1 cut(s) 1301
SstI GAGCTC 1 cut(s) 1129
StyI CCWWGG 2 cut(s) 456, 492
TaaI ACNGT 2 cut(s) 479, 913
TaiI ACGT 4 cut(s) 198, 264, 715, 1360
TaqI TCGA 7 cut(s) 114, 180, 192, 213, 279, 749, 955
TasI AATT 7 cut(s) 199, 447, 561, 641, 790, 819, 1164
TatI WGTACW 1 cut(s) 135
TauI GCSGC 3 cut(s) 80, 242, 387
TfiI GAWTC 4 cut(s) 68, 182, 863, 1232
Tru1I TTAA 6 cut(s) 297, 417, 722, 915, 1025, 1163
Tru9I TTAA 6 cut(s) 297, 417, 722, 915, 1025, 1163
TscAI CASTG 1 cut(s) 484
TseI GCWGC 6 cut(s) 229, 271, 274, 318, 1338, 1371
TspDTI ATGAA 7 cut(s) 162, 459, 803, 815, 939, 999, 1341
TspRI CASTG 1 cut(s) 484
VpaK11BI GGWCC 1 cut(s) 870
XbaI TCTAGA 1 cut(s) 1300
XceI RCATGY 1 cut(s) 236
XhoI CTCGAG 1 cut(s) 278
XmnI GAANNNNTTC 1 cut(s) 1348
XspI CTAG 1 cut(s) 1301
ZraI GACGTC 2 cut(s) 196, 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.