Rroxscaffold_1G00064250

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0010746
Physical Location & Seq
Reverse (-)
86083058 .. 86088629
5572 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00064250.1

Sequence Viewer

Length: 1386 bp
ATGGATTTGAAAACGACGCCGTTGCGGTTCACTCTGGGCAAGCAGTCGTCTCTGGCGCCGGAGCGTGAATCCGACGCCGGAGACGCGGAGGAGGACGAGGTGGAGGTGATCGATCCCCGAGTGAGGCTTATGTACTTGGCCAATGAAGCCGACTTGGACGGGATCAAGGAGCTTCTCGATTCCGGCATCGACGTCAATTTCCGCGACATCGATAATCGGACGGCGCTGCATGTCGCCGCGTGTCAGGGCTACTCCGACGTCGTTTCGCTGCTGCTTGAGCGCGGAGCTGATGTTAATACTAAAGATCGCTGGGGCAGCACCCCTCTTGCAGATGCTATATACTATAAGAACCATGATGTGATCAAGCTTCTGGAGAAGCGCGGCGCGAAGCCTCTGATGGCACCAATGCGCGTTAAGTATGCGAGAGAAGTTCCCGAATATGAAATTGATCCCAAGGAGCTTGATTTTACTAACAGTGTTGAAATAACCAAGGGAACATTCCATTTAGCATCTTGGCGCGGAATACAGGTTGCTGTGAAAAGGCTTGCGGAGGAACTAATTGTTGACGAGGCTAAAGTGAAGGCATTTACGGATGAGCTTGCATTGCTTCAAAAGATACGGCATCCAAATGTAGTGCAATTTCTGGGTGCAGTAACTCAAAGTAGTCCAATGATGATTGTAACAGAATACTTGCCTAAGGGAGACCTACGTGCATTTTTAAAAAGAAAAGGAGCATTGAAACCATCAACAGCTGTGAGATTTGCACTTGATATTGCAAGGGGAATGAATTATTTGCATGAGAATAAACCAGCACCAATTATTCATCGTGATCTTGAGCCTTCAAATATATTGCGGGATGATTCTGGGACCATAAAAGTTGCTGATTTTGGAGTGAGCAAGTTGCTGACAGTTAAAGAAGATAAGCCTTTGATTTGTCAAGATGCTTCATGTCGATACATAGCTCCTGAGGTTTCCAAGAATGAAGAATATGATACAAAAGTGGATGTATTCTCTTTTGCTTTAATCCTACAAGAGATGATAGAAGGCTGTCCACCATTTTCTGCCAAGCGAGATAGTGAAGTTCCAAAAGTATATGTTGCAAGAGAGCGCCCACCTTTCAGAGCTCCAGTAAAGCGTTATGCACATGGCCTTAGAGAGTTAATTGAGGAGTGCTGGAATGAGAAGCCAGCTAAAAGACCATCGTTTAGGCAAATAATCTTGAGGTTGGAATCTATTTATAACACCATCGGCCATAAAAGGCGTTGGAAGCATCGCCGTGAATATGGAACTGACGATGTTACGCTGAAGCAGAAGTACTCTGGTATTGCATTTTGTGAGATAGGTAACTTTCAGACAAAGCAAGCATGGAACTGGAATATGAAATAA

Protein Analysis

461

Amino Acids

52.58

Weight (kDa)

7.61

Isoelectric Point (pI)

45.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 43 - 130 1.7e-16 Ankyrin repeats (3 copies)
Ank_4 PF13637 43 - 92 2.2e-06 Ankyrin repeats (many copies)
Ank_5 PF13857 58 - 109 8.7e-09 Ankyrin repeats (many copies)
Ank PF00023 71 - 103 9e-09 Ankyrin repeat
PK_Tyr_Ser-Thr PF07714 161 - 408 4.6e-55 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 172 - 406 5.4e-50 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010746)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18950 AT4G18950
fragaria_vesca FvH4_3g06740 FvH4_3g06740 FvH4_3g06740 FvH4_3g06740 FvH4_3g06740 FvH4_3g06740
malus_domestica MD05G1293800.v1.1 MD10G1271100.v1.1
prunus_persica Prupe.4G070400_v2.0.a1
pyrus_communis pycom05g27110 pycom10g22690
rosa_chinensis RchiOBHm_Chr5g0012421
rosa_laevigata RLG00000031912
rosa_multiflora Rmu_sc0003469.1_g000008
rosa_roxburghii Rroxscaffold_1G00064250
rosa_rugosa Rorug05G0000900
rosa_samantha Rh5BG091700
rosa_wichuraiana Rw5G008350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1239
AatII GACGTC 2 cut(s) 195, 261
AccB1I GGYRCC 2 cut(s) 55, 400
AccII CGCG 8 cut(s) 86, 204, 239, 282, 381, 386, 411, 519
AciI CCGC 9 cut(s) 25, 86, 202, 237, 282, 381, 519, 548, 853
AclWI GGATC 3 cut(s) 107, 170, 443
AcoI YGGCCR 2 cut(s) 138, 1249
AcuI CTGAAG 1 cut(s) 1325
AcyI GRCGYC 5 cut(s) 17, 56, 75, 192, 258
AfaI GTAC 2 cut(s) 134, 1316
AfiI CCNNNNNNNGG 1 cut(s) 123
AgsI TTSAA 5 cut(s) 10, 482, 611, 739, 843
AjuI GAANNNNNNNTTGG 2 cut(s) 482, 514
AluBI AGCT 9 cut(s) 172, 287, 367, 460, 598, 752, 962, 1124, 1190
AluI AGCT 9 cut(s) 172, 287, 367, 460, 598, 752, 962, 1124, 1190
Alw21I GWGCWC 1 cut(s) 1126
Alw26I GTCTC 3 cut(s) 54, 75, 696
AlwI GGATC 3 cut(s) 107, 170, 443
Ama87I CYCGRG 1 cut(s) 117
AoxI GGCC 3 cut(s) 138, 1147, 1249
ApeKI GCWGC 4 cut(s) 226, 268, 271, 315
AspLEI GCGC 8 cut(s) 58, 226, 282, 381, 386, 411, 519, 1110
AspS9I GGNCC 1 cut(s) 867
AsuHPI GGTGA 1 cut(s) 118
AvaI CYCGRG 1 cut(s) 117
AvaII GGWCC 1 cut(s) 867
AxyI CCTNAGG 2 cut(s) 696, 966
BaeI ACNNNNGTAYC 2 cut(s) 984, 1017
BalI TGGCCA 1 cut(s) 140
BanI GGYRCC 2 cut(s) 55, 400
BanII GRGCYC 1 cut(s) 1126
BarI GAAGNNNNNNTAC 2 cut(s) 1298, 1330
Bbv12I GWGCWC 1 cut(s) 1126
BbvI GCAGC 4 cut(s) 213, 255, 258, 327
BccI CCATC 4 cut(s) 391, 751, 1207, 1253
BceAI ACGGC 4 cut(s) 4, 237, 635, 1260
BcgI CGANNNNNNTGC 2 cut(s) 4, 38
BclI TGATCA 1 cut(s) 360
BcoDI GTCTC 3 cut(s) 54, 75, 696
BfoI RGCGCY 3 cut(s) 59, 227, 1111
BisI GCNGC 6 cut(s) 227, 237, 269, 272, 316, 382
BlsI GCNGC 6 cut(s) 228, 238, 270, 273, 317, 383
BmcAI AGTACT 1 cut(s) 1316
Bme18I GGWCC 1 cut(s) 867
BmeT110I CYCGRG 1 cut(s) 117
BmgT120I GGNCC 1 cut(s) 867
BmiI GGNNCC 3 cut(s) 57, 402, 868
BmsI GCATC 6 cut(s) 195, 322, 518, 631, 931, 1279
BpmI CTGGAG 2 cut(s) 392, 1110
BpuEI CTTGAG 3 cut(s) 296, 854, 1240
Bsa29I ATCGAT 2 cut(s) 111, 210
BsaAI YACGTR 1 cut(s) 710
BsaHI GRCGYC 5 cut(s) 17, 56, 75, 192, 258
BsaI GGTCTC 1 cut(s) 696
BsaJI CCNNGG 2 cut(s) 453, 489
BsaXI ACNNNNNCTCC 2 cut(s) 276, 306
Bsc4I CCNNNNNNNGG 1 cut(s) 123
Bse1I ACTGG 2 cut(s) 1127, 1376
Bse21I CCTNAGG 2 cut(s) 696, 966
Bse3DI GCAATG 1 cut(s) 602
BseCI ATCGAT 2 cut(s) 111, 210
BseDI CCNNGG 2 cut(s) 453, 489
BseGI GGATG 4 cut(s) 598, 622, 862, 1009
BseLI CCNNNNNNNGG 1 cut(s) 123
BseMI GCAATG 1 cut(s) 602
BseMII CTCAG 1 cut(s) 957
BseNI ACTGG 2 cut(s) 1127, 1376
BseRI GAGGAG 2 cut(s) 104, 1181
BseXI GCAGC 4 cut(s) 213, 255, 258, 327
BseYI CCCAGC 1 cut(s) 309
BsgI GTGCAG 1 cut(s) 669
Bsh1236I CGCG 8 cut(s) 86, 204, 239, 282, 381, 386, 411, 519
BshFI GGCC 3 cut(s) 140, 1149, 1251
BshNI GGYRCC 2 cut(s) 55, 400
BshVI ATCGAT 2 cut(s) 111, 210
BsiHKAI GWGCWC 1 cut(s) 1126
BsiHKCI CYCGRG 1 cut(s) 117
BsiSI CCGG 3 cut(s) 59, 78, 183
BslFI GGGAC 1 cut(s) 880
BslI CCNNNNNNNGG 1 cut(s) 123
BsmAI GTCTC 3 cut(s) 54, 75, 696
BsmBI CGTCTC 2 cut(s) 54, 75
BsmFI GGGAC 1 cut(s) 880
BsnI GGCC 3 cut(s) 140, 1149, 1251
Bso31I GGTCTC 1 cut(s) 696
BsoBI CYCGRG 1 cut(s) 117
Bsp1286I GDGCHC 1 cut(s) 1126
Bsp143I GATC 7 cut(s) 108, 112, 162, 304, 360, 448, 829
BspACI CCGC 9 cut(s) 25, 86, 202, 237, 282, 381, 519, 548, 853
BspANI GGCC 3 cut(s) 140, 1149, 1251
BspCNI CTCAG 1 cut(s) 958
BspDI ATCGAT 2 cut(s) 111, 210
BspFNI CGCG 8 cut(s) 86, 204, 239, 282, 381, 386, 411, 519
BspLI GGNNCC 3 cut(s) 57, 402, 868
BspPI GGATC 3 cut(s) 107, 170, 443
BspT107I GGYRCC 2 cut(s) 55, 400
BspTNI GGTCTC 1 cut(s) 696
BsrDI GCAATG 1 cut(s) 602
BsrI ACTGG 2 cut(s) 1127, 1376
BssECI CCNNGG 2 cut(s) 453, 489
BssMI GATC 7 cut(s) 108, 112, 162, 304, 360, 448, 829
BssNI GRCGYC 5 cut(s) 17, 56, 75, 192, 258
BssT1I CCWWGG 2 cut(s) 453, 489
Bst4CI ACNGT 2 cut(s) 476, 910
BstACI GRCGYC 5 cut(s) 17, 56, 75, 192, 258
BstBAI YACGTR 1 cut(s) 710
BstC8I GCNNGC 5 cut(s) 41, 546, 600, 1188, 1362
BstDEI CTNAG 3 cut(s) 696, 966, 1151
BstF5I GGATG 4 cut(s) 598, 622, 862, 1009
BstFNI CGCG 8 cut(s) 86, 204, 239, 282, 381, 386, 411, 519
BstH2I RGCGCY 3 cut(s) 59, 227, 1111
BstHHI GCGC 8 cut(s) 58, 226, 282, 381, 386, 411, 519, 1110
BstKTI GATC 7 cut(s) 111, 115, 165, 307, 363, 451, 832
BstMAI GTCTC 3 cut(s) 54, 75, 696
BstMBI GATC 7 cut(s) 108, 112, 162, 304, 360, 448, 829
BstMWI GCNNNNNNNGC 6 cut(s) 83, 146, 277, 315, 604, 1267
BstNSI RCATGY 1 cut(s) 233
BstUI CGCG 8 cut(s) 86, 204, 239, 282, 381, 386, 411, 519
BstV1I GCAGC 4 cut(s) 213, 255, 258, 327
Bsu15I ATCGAT 2 cut(s) 111, 210
Bsu36I CCTNAGG 2 cut(s) 696, 966
BsuRI GGCC 3 cut(s) 140, 1149, 1251
BsuTUI ATCGAT 2 cut(s) 111, 210
BtgZI GCGATG 1 cut(s) 1256
BtsCI GGATG 4 cut(s) 598, 622, 862, 1009
BtsIMutI CAGTG 1 cut(s) 481
Cac8I GCNNGC 5 cut(s) 41, 546, 600, 1188, 1362
CfoI GCGC 8 cut(s) 58, 226, 282, 381, 386, 411, 519, 1110
Cfr13I GGNCC 1 cut(s) 867
ClaI ATCGAT 2 cut(s) 111, 210
CseI GACGC 3 cut(s) 25, 83, 92
Csp6I GTAC 2 cut(s) 133, 1315
CviAII CATG 6 cut(s) 230, 353, 797, 948, 1145, 1365
CviQI GTAC 2 cut(s) 133, 1315
DdeI CTNAG 3 cut(s) 696, 966, 1151
DinI GGCGCC 1 cut(s) 57
DpnI GATC 7 cut(s) 110, 114, 164, 306, 362, 450, 831
DpnII GATC 7 cut(s) 108, 112, 162, 304, 360, 448, 829
DraI TTTAAA 1 cut(s) 720
EaeI YGGCCR 2 cut(s) 138, 1249
Ecl136II GAGCTC 1 cut(s) 1124
Eco130I CCWWGG 2 cut(s) 453, 489
Eco24I GRGCYC 1 cut(s) 1126
Eco31I GGTCTC 1 cut(s) 696
Eco47I GGWCC 1 cut(s) 867
Eco53kI GAGCTC 1 cut(s) 1124
Eco57I CTGAAG 1 cut(s) 1325
Eco81I CCTNAGG 2 cut(s) 696, 966
Eco88I CYCGRG 1 cut(s) 117
EcoICRI GAGCTC 1 cut(s) 1124
EcoT14I CCWWGG 2 cut(s) 453, 489
EcoT38I GRGCYC 1 cut(s) 1126
EgeI GGCGCC 1 cut(s) 57
EheI GGCGCC 1 cut(s) 57
ErhI CCWWGG 2 cut(s) 453, 489
Esp3I CGTCTC 2 cut(s) 54, 75
FaeI CATG 6 cut(s) 233, 356, 800, 951, 1148, 1368
FaqI GGGAC 1 cut(s) 880
FatI CATG 6 cut(s) 229, 352, 796, 947, 1144, 1364
FauI CCCGC 1 cut(s) 846
FbaI TGATCA 1 cut(s) 360
Fnu4HI GCNGC 6 cut(s) 227, 237, 269, 272, 316, 382
FokI GGATG 4 cut(s) 605, 609, 869, 1016
FriOI GRGCYC 1 cut(s) 1126
Fsp4HI GCNGC 6 cut(s) 227, 237, 269, 272, 316, 382
GlaI GCGC 8 cut(s) 57, 225, 281, 380, 385, 410, 518, 1109
GluI GCNGC 6 cut(s) 227, 237, 269, 272, 316, 382
GsaI CCCAGC 1 cut(s) 313
GsuI CTGGAG 2 cut(s) 392, 1110
HaeII RGCGCY 3 cut(s) 59, 227, 1111
HaeIII GGCC 3 cut(s) 140, 1149, 1251
HapII CCGG 3 cut(s) 59, 78, 183
HgaI GACGC 3 cut(s) 25, 83, 92
HhaI GCGC 8 cut(s) 58, 226, 282, 381, 386, 411, 519, 1110
Hin1I GRCGYC 5 cut(s) 17, 56, 75, 192, 258
Hin1II CATG 6 cut(s) 233, 356, 800, 951, 1148, 1368
Hin6I GCGC 8 cut(s) 56, 224, 280, 379, 384, 409, 517, 1108
HinP1I GCGC 8 cut(s) 56, 224, 280, 379, 384, 409, 517, 1108
HincII GTYRAC 1 cut(s) 565
HindII GTYRAC 1 cut(s) 565
HindIII AAGCTT 1 cut(s) 365
HinfI GANTC 4 cut(s) 68, 179, 860, 1229
HpaII CCGG 3 cut(s) 59, 78, 183
HphI GGTGA 1 cut(s) 118
Hpy166II GTNNAC 3 cut(s) 30, 565, 1052
Hpy188I TCNGA 6 cut(s) 73, 219, 256, 396, 1121, 1353
Hpy188III TCNNGA 8 cut(s) 176, 371, 434, 827, 833, 938, 965, 1219
Hpy8I GTNNAC 3 cut(s) 30, 565, 1052
Hpy99I CGWCG 5 cut(s) 19, 77, 194, 260, 263
HpyAV CCTTC 3 cut(s) 574, 849, 1037
HpyCH4III ACNGT 2 cut(s) 476, 910
HpyCH4IV ACGT 3 cut(s) 192, 258, 709
HpyF10VI GCNNNNNNNGC 6 cut(s) 83, 146, 277, 315, 604, 1267
HpyF3I CTNAG 3 cut(s) 696, 966, 1151
HpySE526I ACGT 3 cut(s) 192, 258, 709
Hsp92I GRCGYC 5 cut(s) 17, 56, 75, 192, 258
Hsp92II CATG 6 cut(s) 233, 356, 800, 951, 1148, 1368
HspAI GCGC 8 cut(s) 56, 224, 280, 379, 384, 409, 517, 1108
KasI GGCGCC 1 cut(s) 55
Ksp22I TGATCA 1 cut(s) 360
Kzo9I GATC 7 cut(s) 108, 112, 162, 304, 360, 448, 829
LmnI GCTCC 7 cut(s) 61, 169, 284, 457, 731, 967, 1129
Lsp1109I GCAGC 4 cut(s) 213, 255, 258, 327
LweI GCATC 6 cut(s) 195, 322, 518, 631, 931, 1279
MaeII ACGT 3 cut(s) 192, 258, 709
MaeIII GTNAC 4 cut(s) 652, 679, 1297, 1343
MalI GATC 7 cut(s) 110, 114, 164, 306, 362, 450, 831
MboI GATC 7 cut(s) 108, 112, 162, 304, 360, 448, 829
MboII GAAGA 2 cut(s) 929, 995
MhlI GDGCHC 1 cut(s) 1126
MlsI TGGCCA 1 cut(s) 140
MluCI AATT 7 cut(s) 196, 444, 558, 638, 787, 816, 1161
MluNI TGGCCA 1 cut(s) 140
Mly113I GGCGCC 1 cut(s) 56
MmeI TCCRAC 4 cut(s) 96, 279, 1206, 1244
Mox20I TGGCCA 1 cut(s) 140
MscI TGGCCA 1 cut(s) 140
MseI TTAA 6 cut(s) 294, 414, 719, 912, 1022, 1160
MslI CAYNNNNRTG 2 cut(s) 627, 1275
Msp20I TGGCCA 1 cut(s) 140
MspA1I CMGCKG 1 cut(s) 752
MspI CCGG 3 cut(s) 59, 78, 183
MteI GCGCNGCGC 1 cut(s) 382
MvnI CGCG 8 cut(s) 86, 204, 239, 282, 381, 386, 411, 519
MwoI GCNNNNNNNGC 6 cut(s) 83, 146, 277, 315, 604, 1267
NarI GGCGCC 1 cut(s) 56
NdeII GATC 7 cut(s) 108, 112, 162, 304, 360, 448, 829
NlaIII CATG 6 cut(s) 233, 356, 800, 951, 1148, 1368
NlaIV GGNNCC 3 cut(s) 57, 402, 868
NspI RCATGY 1 cut(s) 233
PfeI GAWTC 4 cut(s) 68, 179, 860, 1229
PkrI GCNGC 6 cut(s) 228, 238, 270, 273, 317, 383
PluTI GGCGCC 1 cut(s) 59
Ppu21I YACGTR 1 cut(s) 710
PsiI TTATAA 1 cut(s) 1239
Psp124BI GAGCTC 1 cut(s) 1126
PspFI CCCAGC 1 cut(s) 309
PspN4I GGNNCC 3 cut(s) 57, 402, 868
PspPI GGNCC 1 cut(s) 867
PvuII CAGCTG 1 cut(s) 752
RsaI GTAC 2 cut(s) 134, 1316
RsaNI GTAC 2 cut(s) 133, 1315
RseI CAYNNNNRTG 2 cut(s) 627, 1275
SacI GAGCTC 1 cut(s) 1126
SaqAI TTAA 6 cut(s) 294, 414, 719, 912, 1022, 1160
SatI GCNGC 6 cut(s) 227, 237, 269, 272, 316, 382
Sau3AI GATC 7 cut(s) 108, 112, 162, 304, 360, 448, 829
Sau96I GGNCC 1 cut(s) 867
ScaI AGTACT 1 cut(s) 1316
SduI GDGCHC 1 cut(s) 1126
SfaNI GCATC 6 cut(s) 195, 322, 518, 631, 931, 1279
SfoI GGCGCC 1 cut(s) 57
SinI GGWCC 1 cut(s) 867
SmiMI CAYNNNNRTG 2 cut(s) 627, 1275
SmlI CTYRAG 3 cut(s) 275, 833, 1219
SmoI CTYRAG 3 cut(s) 275, 833, 1219
Sse9I AATT 7 cut(s) 196, 444, 558, 638, 787, 816, 1161
SsiI CCGC 9 cut(s) 25, 86, 202, 237, 282, 381, 519, 548, 853
SspDI GGCGCC 1 cut(s) 55
SstI GAGCTC 1 cut(s) 1126
StyI CCWWGG 2 cut(s) 453, 489
TaaI ACNGT 2 cut(s) 476, 910
TaiI ACGT 3 cut(s) 195, 261, 712
TaqI TCGA 5 cut(s) 111, 177, 189, 210, 952
TasI AATT 7 cut(s) 196, 444, 558, 638, 787, 816, 1161
TatI WGTACW 2 cut(s) 132, 1314
TauI GCSGC 2 cut(s) 239, 384
TfiI GAWTC 4 cut(s) 68, 179, 860, 1229
Tru1I TTAA 6 cut(s) 294, 414, 719, 912, 1022, 1160
Tru9I TTAA 6 cut(s) 294, 414, 719, 912, 1022, 1160
TscAI CASTG 1 cut(s) 481
TseI GCWGC 4 cut(s) 226, 268, 271, 315
TspDTI ATGAA 6 cut(s) 159, 456, 800, 812, 936, 996
TspGWI ACGGA 1 cut(s) 605
TspRI CASTG 1 cut(s) 481
VpaK11BI GGWCC 1 cut(s) 867
XceI RCATGY 1 cut(s) 233
ZraI GACGTC 2 cut(s) 193, 259
ZrmI AGTACT 1 cut(s) 1316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.