RchiOBHm_Chr5g0012421

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0010746
Physical Location & Seq
Forward (+)
8334373 .. 8339985
5613 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29299

Sequence Viewer

Length: 1377 bp
ATGGATTTGAAAACGACGCCGTTGCGGTTCACTCTGGGCAAGCAGTCGTCTCTGGCGCCGGAGCGTGAATCCGACGCCGGAGACGCGGAGGAGGACGAGGTGGAGGTGATCGATCCCCGAGTGAGGCTTATGTACTTGGCCAATGAAGCCGACTTGGACGGGATCAAGGAGCTTCTCGATTCCGGCATCGACGTCAATTTCCGCGACATCGATAATCGGACGGCGCTGCATGTCGCCGCGTGTCAGGGCTACTCCGACGTCGTTTCGCTGCTGCTCGAGCGCGGAGCTGATGTTAATACTAAAGATCGCTGGGGCAGCTCCCCTCTTGCAGATGCTATATACTATAAGAACCATGATGTGATCAAGCTTCTGGAGAAGCGCGGCGCGAAGCCTCTGATGGCACCAATGCGCGTTAAGTATGCAAGAGAAGTTCCCGAATATGAAATTGATCCCAAGGAGCTTGATTTTACTAACAGTGTTGAAATAACCAAGGGAACATTCCATTTAGCATCTTGGCGCGGAATACAGGTGGCTGTGAAAAGGCTTGCGGAGGAACTAATTGTTGACGAGGCTAAAGTGAAGGCATTTACGGATGAGCTTACATTGCTTCAAAAGATACGGCATCCAAATGTAGTGCAATTTCTGGGTGCAGTAACTCAAAGTAGTCCAATGATGATTGTAACAGAATACTTGCCTAAGGGAGACCTACGTGCATTTTTAAAAAGAAAAGGAGCATTGAAACCGTCGACAGCTGTGAGATTTGCACTTGATATTGCAAGGGGAATGAATTATTTGCATGAGAATAAACCAGCACCAATTATTCATCGTGATCTTGAGCCTTCAAATATATTGCGAGATGATTCTGGGACCATAAAAGTTGCTGATTTTGGAGTGAGCAAGTTGCTGACGGTTAAAGAAGATAAGCCTTTGATTTGTCAAGATGCTTCATGTCGATACATAGCTCCTGAGGTTTCTAAGAATGAAGAATATGATACAAAAGTGGATGTATTCTCTTTTGCTTTAATCCTACAAGAGATGATAGAAGGCTGTCCACCATTTTCTGCCAAGCAAGATAGTGAAGTTCCAAAAGTATATGTTGCAAGAGAGCGCCCACCTTTCAGAGCTCCAGTAAAGCGTTATGCACATGGCCTTAGAGAGTTAATTGAGGAGTGCTGGAATGAGAAGCCAGCTAAAAGACCATCGTTTAGGCAAATAATCTTGAGGTTGGAATCTATTTATAACACCATTGGCCATAAAAGGCGTTGGAAGGTTAGACCATTGAAATGCTTTCCAAATCTAGAAGCCATGTTGAGGAAAGATCATATCAGTCTGAGCAGCAGAAGCCGTTCATCACGTTCCTCTGCTAGCAGCATATAA

Protein Analysis

458

Amino Acids

51.92

Weight (kDa)

8.33

Isoelectric Point (pI)

53.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 43 - 130 1.1e-15 Ankyrin repeats (3 copies)
Ank_4 PF13637 43 - 92 2.2e-06 Ankyrin repeats (many copies)
Ank_5 PF13857 58 - 109 3.2e-08 Ankyrin repeats (many copies)
Ank PF00023 71 - 103 9e-09 Ankyrin repeat
PK_Tyr_Ser-Thr PF07714 161 - 408 3.1e-55 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 172 - 406 3.5e-50 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010746)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18950 AT4G18950
fragaria_vesca FvH4_3g06740 FvH4_3g06740 FvH4_3g06740 FvH4_3g06740 FvH4_3g06740 FvH4_3g06740
malus_domestica MD05G1293800.v1.1 MD10G1271100.v1.1
prunus_persica Prupe.4G070400_v2.0.a1
pyrus_communis pycom05g27110 pycom10g22690
rosa_chinensis RchiOBHm_Chr5g0012421
rosa_laevigata RLG00000031912
rosa_multiflora Rmu_sc0003469.1_g000008
rosa_roxburghii Rroxscaffold_1G00064250
rosa_rugosa Rorug05G0000900
rosa_samantha Rh5BG091700
rosa_wichuraiana Rw5G008350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1239
AatII GACGTC 2 cut(s) 195, 261
AccB1I GGYRCC 2 cut(s) 55, 400
AccI GTMKAC 1 cut(s) 746
AccII CGCG 8 cut(s) 86, 204, 239, 282, 381, 386, 411, 519
AciI CCGC 8 cut(s) 25, 86, 202, 237, 282, 381, 519, 548
AclWI GGATC 3 cut(s) 107, 170, 443
AcoI YGGCCR 2 cut(s) 138, 1249
AcyI GRCGYC 5 cut(s) 17, 56, 75, 192, 258
AfaI GTAC 1 cut(s) 134
AfiI CCNNNNNNNGG 2 cut(s) 123, 1311
AgsI TTSAA 6 cut(s) 10, 482, 611, 739, 843, 1282
AjuI GAANNNNNNNTTGG 2 cut(s) 482, 514
Alw21I GWGCWC 1 cut(s) 1126
Alw26I GTCTC 3 cut(s) 54, 75, 696
AlwI GGATC 3 cut(s) 107, 170, 443
Ama87I CYCGRG 2 cut(s) 117, 275
AoxI GGCC 3 cut(s) 138, 1147, 1249
ApeKI GCWGC 6 cut(s) 226, 268, 271, 315, 1335, 1368
Asp700I GAANNNNTTC 1 cut(s) 1345
AspLEI GCGC 8 cut(s) 58, 226, 282, 381, 386, 411, 519, 1110
AspS9I GGNCC 1 cut(s) 867
AsuHPI GGTGA 1 cut(s) 118
AsuNHI GCTAGC 1 cut(s) 1364
AvaI CYCGRG 2 cut(s) 117, 275
AvaII GGWCC 1 cut(s) 867
AxyI CCTNAGG 2 cut(s) 696, 966
BaeI ACNNNNGTAYC 2 cut(s) 984, 1017
BalI TGGCCA 2 cut(s) 140, 1251
BanI GGYRCC 2 cut(s) 55, 400
BanII GRGCYC 1 cut(s) 1126
Bbv12I GWGCWC 1 cut(s) 1126
BbvI GCAGC 5 cut(s) 213, 255, 258, 327, 1347
BccI CCATC 2 cut(s) 391, 1207
BceAI ACGGC 4 cut(s) 4, 237, 635, 1329
BcgI CGANNNNNNTGC 2 cut(s) 4, 38
BclI TGATCA 1 cut(s) 360
BcoDI GTCTC 3 cut(s) 54, 75, 696
BfaI CTAG 2 cut(s) 1298, 1365
BfoI RGCGCY 3 cut(s) 59, 227, 1111
BisI GCNGC 8 cut(s) 227, 237, 269, 272, 316, 382, 1336, 1369
BlsI GCNGC 8 cut(s) 228, 238, 270, 273, 317, 383, 1337, 1370
Bme18I GGWCC 1 cut(s) 867
BmeT110I CYCGRG 2 cut(s) 117, 275
BmgT120I GGNCC 1 cut(s) 867
BmiI GGNNCC 3 cut(s) 57, 402, 868
BmsI GCATC 5 cut(s) 195, 322, 518, 631, 931
BmtI GCTAGC 1 cut(s) 1368
BpmI CTGGAG 2 cut(s) 392, 1110
BpuEI CTTGAG 2 cut(s) 854, 1240
Bsa29I ATCGAT 2 cut(s) 111, 210
BsaAI YACGTR 1 cut(s) 710
BsaHI GRCGYC 5 cut(s) 17, 56, 75, 192, 258
BsaI GGTCTC 1 cut(s) 696
BsaJI CCNNGG 2 cut(s) 453, 489
BsaXI ACNNNNNCTCC 2 cut(s) 276, 306
Bsc4I CCNNNNNNNGG 2 cut(s) 123, 1311
Bse1I ACTGG 1 cut(s) 1127
Bse21I CCTNAGG 2 cut(s) 696, 966
Bse3DI GCAATG 1 cut(s) 602
BseCI ATCGAT 2 cut(s) 111, 210
BseDI CCNNGG 2 cut(s) 453, 489
BseGI GGATG 3 cut(s) 598, 622, 1009
BseLI CCNNNNNNNGG 2 cut(s) 123, 1311
BseMI GCAATG 1 cut(s) 602
BseMII CTCAG 2 cut(s) 957, 1322
BseNI ACTGG 1 cut(s) 1127
BseRI GAGGAG 2 cut(s) 104, 1181
BseXI GCAGC 5 cut(s) 213, 255, 258, 327, 1347
BseYI CCCAGC 1 cut(s) 309
BsgI GTGCAG 1 cut(s) 669
Bsh1236I CGCG 8 cut(s) 86, 204, 239, 282, 381, 386, 411, 519
BshFI GGCC 3 cut(s) 140, 1149, 1251
BshNI GGYRCC 2 cut(s) 55, 400
BshVI ATCGAT 2 cut(s) 111, 210
BsiHKAI GWGCWC 1 cut(s) 1126
BsiHKCI CYCGRG 2 cut(s) 117, 275
BsiSI CCGG 3 cut(s) 59, 78, 183
BslFI GGGAC 1 cut(s) 880
BslI CCNNNNNNNGG 2 cut(s) 123, 1311
BsmAI GTCTC 3 cut(s) 54, 75, 696
BsmBI CGTCTC 2 cut(s) 54, 75
BsmFI GGGAC 1 cut(s) 880
BsnI GGCC 3 cut(s) 140, 1149, 1251
Bso31I GGTCTC 1 cut(s) 696
BsoBI CYCGRG 2 cut(s) 117, 275
Bsp1286I GDGCHC 1 cut(s) 1126
Bsp143I GATC 8 cut(s) 108, 112, 162, 304, 360, 448, 829, 1318
BspACI CCGC 8 cut(s) 25, 86, 202, 237, 282, 381, 519, 548
BspANI GGCC 3 cut(s) 140, 1149, 1251
BspCNI CTCAG 2 cut(s) 958, 1323
BspDI ATCGAT 2 cut(s) 111, 210
BspFNI CGCG 8 cut(s) 86, 204, 239, 282, 381, 386, 411, 519
BspLI GGNNCC 3 cut(s) 57, 402, 868
BspOI GCTAGC 1 cut(s) 1368
BspPI GGATC 3 cut(s) 107, 170, 443
BspT107I GGYRCC 2 cut(s) 55, 400
BspTNI GGTCTC 1 cut(s) 696
BsrDI GCAATG 1 cut(s) 602
BsrI ACTGG 1 cut(s) 1127
BssECI CCNNGG 2 cut(s) 453, 489
BssMI GATC 8 cut(s) 108, 112, 162, 304, 360, 448, 829, 1318
BssNI GRCGYC 5 cut(s) 17, 56, 75, 192, 258
BssT1I CCWWGG 2 cut(s) 453, 489
Bst4CI ACNGT 3 cut(s) 476, 744, 910
BstACI GRCGYC 5 cut(s) 17, 56, 75, 192, 258
BstBAI YACGTR 1 cut(s) 710
BstC8I GCNNGC 4 cut(s) 41, 546, 1188, 1366
BstDEI CTNAG 5 cut(s) 696, 966, 975, 1151, 1331
BstF5I GGATG 3 cut(s) 598, 622, 1009
BstFNI CGCG 8 cut(s) 86, 204, 239, 282, 381, 386, 411, 519
BstH2I RGCGCY 3 cut(s) 59, 227, 1111
BstHHI GCGC 8 cut(s) 58, 226, 282, 381, 386, 411, 519, 1110
BstKTI GATC 8 cut(s) 111, 115, 165, 307, 363, 451, 832, 1321
BstMAI GTCTC 3 cut(s) 54, 75, 696
BstMBI GATC 8 cut(s) 108, 112, 162, 304, 360, 448, 829, 1318
BstMWI GCNNNNNNNGC 6 cut(s) 83, 146, 277, 315, 604, 1341
BstNSI RCATGY 1 cut(s) 233
BstUI CGCG 8 cut(s) 86, 204, 239, 282, 381, 386, 411, 519
BstV1I GCAGC 5 cut(s) 213, 255, 258, 327, 1347
Bsu15I ATCGAT 2 cut(s) 111, 210
Bsu36I CCTNAGG 2 cut(s) 696, 966
BsuRI GGCC 3 cut(s) 140, 1149, 1251
BsuTUI ATCGAT 2 cut(s) 111, 210
BtsCI GGATG 3 cut(s) 598, 622, 1009
BtsIMutI CAGTG 1 cut(s) 481
Cac8I GCNNGC 4 cut(s) 41, 546, 1188, 1366
CfoI GCGC 8 cut(s) 58, 226, 282, 381, 386, 411, 519, 1110
Cfr13I GGNCC 1 cut(s) 867
ClaI ATCGAT 2 cut(s) 111, 210
CseI GACGC 3 cut(s) 25, 83, 92
Csp6I GTAC 1 cut(s) 133
CviAII CATG 6 cut(s) 230, 353, 797, 948, 1145, 1306
CviQI GTAC 1 cut(s) 133
DdeI CTNAG 5 cut(s) 696, 966, 975, 1151, 1331
DinI GGCGCC 1 cut(s) 57
DpnI GATC 8 cut(s) 110, 114, 164, 306, 362, 450, 831, 1320
DpnII GATC 8 cut(s) 108, 112, 162, 304, 360, 448, 829, 1318
DraI TTTAAA 1 cut(s) 720
EaeI YGGCCR 2 cut(s) 138, 1249
Ecl136II GAGCTC 1 cut(s) 1124
Eco130I CCWWGG 2 cut(s) 453, 489
Eco24I GRGCYC 1 cut(s) 1126
Eco31I GGTCTC 1 cut(s) 696
Eco47I GGWCC 1 cut(s) 867
Eco53kI GAGCTC 1 cut(s) 1124
Eco81I CCTNAGG 2 cut(s) 696, 966
Eco88I CYCGRG 2 cut(s) 117, 275
EcoICRI GAGCTC 1 cut(s) 1124
EcoT14I CCWWGG 2 cut(s) 453, 489
EcoT38I GRGCYC 1 cut(s) 1126
EgeI GGCGCC 1 cut(s) 57
EheI GGCGCC 1 cut(s) 57
ErhI CCWWGG 2 cut(s) 453, 489
Esp3I CGTCTC 2 cut(s) 54, 75
FaeI CATG 6 cut(s) 233, 356, 800, 951, 1148, 1309
FaqI GGGAC 1 cut(s) 880
FatI CATG 6 cut(s) 229, 352, 796, 947, 1144, 1305
FbaI TGATCA 1 cut(s) 360
FblI GTMKAC 1 cut(s) 746
Fnu4HI GCNGC 8 cut(s) 227, 237, 269, 272, 316, 382, 1336, 1369
FokI GGATG 3 cut(s) 605, 609, 1016
FriOI GRGCYC 1 cut(s) 1126
Fsp4HI GCNGC 8 cut(s) 227, 237, 269, 272, 316, 382, 1336, 1369
FspBI CTAG 2 cut(s) 1298, 1365
GlaI GCGC 8 cut(s) 57, 225, 281, 380, 385, 410, 518, 1109
GluI GCNGC 8 cut(s) 227, 237, 269, 272, 316, 382, 1336, 1369
GsaI CCCAGC 1 cut(s) 313
GsuI CTGGAG 2 cut(s) 392, 1110
HaeII RGCGCY 3 cut(s) 59, 227, 1111
HaeIII GGCC 3 cut(s) 140, 1149, 1251
HapII CCGG 3 cut(s) 59, 78, 183
HgaI GACGC 3 cut(s) 25, 83, 92
HhaI GCGC 8 cut(s) 58, 226, 282, 381, 386, 411, 519, 1110
Hin1I GRCGYC 5 cut(s) 17, 56, 75, 192, 258
Hin1II CATG 6 cut(s) 233, 356, 800, 951, 1148, 1309
Hin6I GCGC 8 cut(s) 56, 224, 280, 379, 384, 409, 517, 1108
HinP1I GCGC 8 cut(s) 56, 224, 280, 379, 384, 409, 517, 1108
HincII GTYRAC 2 cut(s) 565, 747
HindII GTYRAC 2 cut(s) 565, 747
HindIII AAGCTT 1 cut(s) 365
HinfI GANTC 4 cut(s) 68, 179, 860, 1229
HpaII CCGG 3 cut(s) 59, 78, 183
HphI GGTGA 1 cut(s) 118
Hpy166II GTNNAC 4 cut(s) 30, 565, 747, 1052
Hpy188I TCNGA 6 cut(s) 73, 219, 256, 396, 1121, 1332
Hpy188III TCNNGA 9 cut(s) 176, 371, 434, 827, 833, 938, 965, 1219, 1298
Hpy8I GTNNAC 4 cut(s) 30, 565, 747, 1052
Hpy99I CGWCG 6 cut(s) 19, 77, 194, 260, 263, 748
HpyAV CCTTC 4 cut(s) 574, 849, 1037, 1261
HpyCH4III ACNGT 3 cut(s) 476, 744, 910
HpyCH4IV ACGT 4 cut(s) 192, 258, 709, 1354
HpyF10VI GCNNNNNNNGC 6 cut(s) 83, 146, 277, 315, 604, 1341
HpyF3I CTNAG 5 cut(s) 696, 966, 975, 1151, 1331
HpySE526I ACGT 4 cut(s) 192, 258, 709, 1354
Hsp92I GRCGYC 5 cut(s) 17, 56, 75, 192, 258
Hsp92II CATG 6 cut(s) 233, 356, 800, 951, 1148, 1309
HspAI GCGC 8 cut(s) 56, 224, 280, 379, 384, 409, 517, 1108
KasI GGCGCC 1 cut(s) 55
Ksp22I TGATCA 1 cut(s) 360
Kzo9I GATC 8 cut(s) 108, 112, 162, 304, 360, 448, 829, 1318
LmnI GCTCC 8 cut(s) 61, 169, 284, 323, 457, 731, 967, 1129
Lsp1109I GCAGC 5 cut(s) 213, 255, 258, 327, 1347
LweI GCATC 5 cut(s) 195, 322, 518, 631, 931
MaeI CTAG 2 cut(s) 1298, 1365
MaeII ACGT 4 cut(s) 192, 258, 709, 1354
MaeIII GTNAC 2 cut(s) 652, 679
MalI GATC 8 cut(s) 110, 114, 164, 306, 362, 450, 831, 1320
MboI GATC 8 cut(s) 108, 112, 162, 304, 360, 448, 829, 1318
MboII GAAGA 2 cut(s) 929, 995
MhlI GDGCHC 1 cut(s) 1126
MlsI TGGCCA 2 cut(s) 140, 1251
MluCI AATT 7 cut(s) 196, 444, 558, 638, 787, 816, 1161
MluNI TGGCCA 2 cut(s) 140, 1251
Mly113I GGCGCC 1 cut(s) 56
MmeI TCCRAC 4 cut(s) 96, 279, 1206, 1244
Mox20I TGGCCA 2 cut(s) 140, 1251
MroXI GAANNNNTTC 1 cut(s) 1345
MscI TGGCCA 2 cut(s) 140, 1251
MseI TTAA 6 cut(s) 294, 414, 719, 912, 1022, 1160
MslI CAYNNNNRTG 2 cut(s) 627, 1282
Msp20I TGGCCA 2 cut(s) 140, 1251
MspA1I CMGCKG 1 cut(s) 752
MspI CCGG 3 cut(s) 59, 78, 183
MteI GCGCNGCGC 1 cut(s) 382
MvnI CGCG 8 cut(s) 86, 204, 239, 282, 381, 386, 411, 519
MwoI GCNNNNNNNGC 6 cut(s) 83, 146, 277, 315, 604, 1341
NarI GGCGCC 1 cut(s) 56
NdeII GATC 8 cut(s) 108, 112, 162, 304, 360, 448, 829, 1318
NheI GCTAGC 1 cut(s) 1364
NlaIII CATG 6 cut(s) 233, 356, 800, 951, 1148, 1309
NlaIV GGNNCC 3 cut(s) 57, 402, 868
NspI RCATGY 1 cut(s) 233
PaeR7I CTCGAG 1 cut(s) 275
PdmI GAANNNNTTC 1 cut(s) 1345
PfeI GAWTC 4 cut(s) 68, 179, 860, 1229
PkrI GCNGC 8 cut(s) 228, 238, 270, 273, 317, 383, 1337, 1370
PluTI GGCGCC 1 cut(s) 59
Ppu21I YACGTR 1 cut(s) 710
PsiI TTATAA 1 cut(s) 1239
Psp124BI GAGCTC 1 cut(s) 1126
PspFI CCCAGC 1 cut(s) 309
PspN4I GGNNCC 3 cut(s) 57, 402, 868
PspPI GGNCC 1 cut(s) 867
PspXI VCTCGAGB 1 cut(s) 275
PvuII CAGCTG 1 cut(s) 752
RsaI GTAC 1 cut(s) 134
RsaNI GTAC 1 cut(s) 133
RseI CAYNNNNRTG 2 cut(s) 627, 1282
SacI GAGCTC 1 cut(s) 1126
SalI GTCGAC 1 cut(s) 745
SaqAI TTAA 6 cut(s) 294, 414, 719, 912, 1022, 1160
SatI GCNGC 8 cut(s) 227, 237, 269, 272, 316, 382, 1336, 1369
Sau3AI GATC 8 cut(s) 108, 112, 162, 304, 360, 448, 829, 1318
Sau96I GGNCC 1 cut(s) 867
SduI GDGCHC 1 cut(s) 1126
SfaNI GCATC 5 cut(s) 195, 322, 518, 631, 931
SfoI GGCGCC 1 cut(s) 57
Sfr274I CTCGAG 1 cut(s) 275
SinI GGWCC 1 cut(s) 867
SlaI CTCGAG 1 cut(s) 275
SmiMI CAYNNNNRTG 2 cut(s) 627, 1282
SmlI CTYRAG 3 cut(s) 275, 833, 1219
SmoI CTYRAG 3 cut(s) 275, 833, 1219
Sse9I AATT 7 cut(s) 196, 444, 558, 638, 787, 816, 1161
SsiI CCGC 8 cut(s) 25, 86, 202, 237, 282, 381, 519, 548
SspDI GGCGCC 1 cut(s) 55
SspMI CTAG 2 cut(s) 1298, 1365
SstI GAGCTC 1 cut(s) 1126
StyI CCWWGG 2 cut(s) 453, 489
TaaI ACNGT 3 cut(s) 476, 744, 910
TaiI ACGT 4 cut(s) 195, 261, 712, 1357
TaqI TCGA 7 cut(s) 111, 177, 189, 210, 276, 746, 952
TasI AATT 7 cut(s) 196, 444, 558, 638, 787, 816, 1161
TatI WGTACW 1 cut(s) 132
TauI GCSGC 2 cut(s) 239, 384
TfiI GAWTC 4 cut(s) 68, 179, 860, 1229
Tru1I TTAA 6 cut(s) 294, 414, 719, 912, 1022, 1160
Tru9I TTAA 6 cut(s) 294, 414, 719, 912, 1022, 1160
TscAI CASTG 1 cut(s) 481
TseI GCWGC 6 cut(s) 226, 268, 271, 315, 1335, 1368
TspDTI ATGAA 7 cut(s) 159, 456, 800, 812, 936, 996, 1338
TspGWI ACGGA 1 cut(s) 605
TspRI CASTG 1 cut(s) 481
VpaK11BI GGWCC 1 cut(s) 867
XbaI TCTAGA 1 cut(s) 1297
XceI RCATGY 1 cut(s) 233
XhoI CTCGAG 1 cut(s) 275
XmiI GTMKAC 1 cut(s) 746
XmnI GAANNNNTTC 1 cut(s) 1345
XspI CTAG 2 cut(s) 1298, 1365
ZraI GACGTC 2 cut(s) 193, 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.