AT4G21310

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0014095
Physical Location & Seq
Reverse (-)
11339048 .. 11340085
1038 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G21310.2

Sequence Viewer

Length: 507 bp
ATGGCGAGGAATGTAGGGTTCTTCATCTGCATCTTGATCCTAGCCATGGATGTTTCGGCTGGAATTCTCGGCATTGAAGCCGAAATAGCTCAGAACAAGGTGAAGCATTTGAAGATGTGGATATTCGAGTGTAGAGACCCGAGTTACACAGCCTTCAAGTACGGTTTAGCTGCGTGCATCCTTTTGGTGTTAGCCCATGTAACGGCTAATTTTCTTGGTGGGTGTCTCTGTGTTGCCTCAAGACAAGACCTTGAAAAATCCTCTGCCAATAAACAGCTCGCTGTGGCTTCTCTGATATTCACGTGGATCATATTGGCGATTGCATTTTCGATGTTGATAGTAGGGACGATGGCAAACTCAAGATCGAGAAAAAACTGTGGGATATCACACCATCGGGTTCTGTCCATAGGAGGGATCCTCTGTTTCGTTCACGGCCTCTTTGCTGTTGCTTATTACATCTCTGCAACTGCTTCAACACGAGAACAGACAAGCGCAGGCCACGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

168

Amino Acids

18.14

Weight (kDa)

9.08

Isoelectric Point (pI)

38.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 20 - 107 2e-22 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014095)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21310 AT4G21310
fragaria_vesca FvH4_3g02950
malus_domestica MD10G1311300.v1.1
prunus_persica Prupe.4G029600_v2.0.a1
pyrus_communis pycom10g26250
rosa_chinensis RchiOBHm_Chr5g0004561
rosa_laevigata RLG00000031240
rosa_multiflora Rmu_sc0002354.1_g000018 Rmu_sc0003758.1_g000012
rosa_roxburghii Rroxscaffold_1G00070970
rosa_rugosa Rorug04G0412200
rosa_samantha Rh5AG040200 Rh5BG039600 Rh5CG043500 Rh5DG039300
rosa_wichuraiana Rw5G003860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 4 cut(s) 31, 314, 409, 422
AcsI RAATTY 1 cut(s) 63
AcvI CACGTG 1 cut(s) 303
AfaI GTAC 1 cut(s) 161
AfiI CCNNNNNNNGG 3 cut(s) 46, 202, 411
AgsI TTSAA 5 cut(s) 77, 112, 157, 254, 474
AluBI AGCT 3 cut(s) 89, 170, 277
AluI AGCT 3 cut(s) 89, 170, 277
Alw26I GTCTC 2 cut(s) 129, 230
AlwI GGATC 4 cut(s) 31, 314, 409, 422
Ama87I CYCGRG 1 cut(s) 139
AoxI GGCC 2 cut(s) 433, 496
ApeKI GCWGC 1 cut(s) 170
ApoI RAATTY 1 cut(s) 63
AspLEI GCGC 1 cut(s) 494
AsuHPI GGTGA 1 cut(s) 112
AvaI CYCGRG 1 cut(s) 139
BamHI GGATCC 1 cut(s) 414
BauI CACGAG 1 cut(s) 477
BbrPI CACGTG 1 cut(s) 303
BbvI GCAGC 1 cut(s) 157
BccI CCATC 2 cut(s) 343, 399
BceAI ACGGC 2 cut(s) 219, 448
BcoDI GTCTC 2 cut(s) 129, 230
BfaI CTAG 1 cut(s) 41
BisI GCNGC 1 cut(s) 171
BlsI GCNGC 1 cut(s) 172
BmeT110I CYCGRG 1 cut(s) 139
BmiI GGNNCC 1 cut(s) 416
BmsI GCATC 2 cut(s) 39, 186
BplI GAGNNNNNCTC 2 cut(s) 402, 434
BpuEI CTTGAG 2 cut(s) 223, 343
BsaAI YACGTR 1 cut(s) 303
BsaI GGTCTC 1 cut(s) 129
BsaJI CCNNGG 1 cut(s) 45
Bsc4I CCNNNNNNNGG 3 cut(s) 46, 202, 411
BseDI CCNNGG 1 cut(s) 45
BseGI GGATG 2 cut(s) 55, 177
BseLI CCNNNNNNNGG 3 cut(s) 46, 202, 411
BseMII CTCAG 1 cut(s) 104
BseXI GCAGC 1 cut(s) 157
BshFI GGCC 2 cut(s) 435, 498
BsiHKCI CYCGRG 1 cut(s) 139
BslFI GGGAC 1 cut(s) 358
BslI CCNNNNNNNGG 3 cut(s) 46, 202, 411
BsmAI GTCTC 2 cut(s) 129, 230
BsmFI GGGAC 1 cut(s) 358
BsnI GGCC 2 cut(s) 435, 498
Bso31I GGTCTC 1 cut(s) 129
BsoBI CYCGRG 1 cut(s) 139
Bsp143I GATC 4 cut(s) 36, 306, 362, 414
Bsp19I CCATGG 1 cut(s) 45
BspANI GGCC 2 cut(s) 435, 498
BspCNI CTCAG 1 cut(s) 103
BspLI GGNNCC 1 cut(s) 416
BspPI GGATC 4 cut(s) 31, 314, 409, 422
BspTNI GGTCTC 1 cut(s) 129
BssECI CCNNGG 1 cut(s) 45
BssMI GATC 4 cut(s) 36, 306, 362, 414
BssSI CACGAG 1 cut(s) 477
BssT1I CCWWGG 1 cut(s) 45
Bst2BI CACGAG 1 cut(s) 477
Bst4CI ACNGT 2 cut(s) 164, 377
BstBAI YACGTR 1 cut(s) 303
BstC8I GCNNGC 3 cut(s) 175, 279, 496
BstDEI CTNAG 1 cut(s) 90
BstDSI CCRYGG 1 cut(s) 45
BstF5I GGATG 2 cut(s) 55, 177
BstHHI GCGC 1 cut(s) 494
BstKTI GATC 4 cut(s) 39, 309, 365, 417
BstMAI GTCTC 2 cut(s) 129, 230
BstMBI GATC 4 cut(s) 36, 306, 362, 414
BstMWI GCNNNNNNNGC 2 cut(s) 86, 500
BstV1I GCAGC 1 cut(s) 157
BstX2I RGATCY 1 cut(s) 414
BstYI RGATCY 1 cut(s) 414
BsuRI GGCC 2 cut(s) 435, 498
BtgI CCRYGG 1 cut(s) 45
BtsCI GGATG 2 cut(s) 55, 177
Cac8I GCNNGC 3 cut(s) 175, 279, 496
CfoI GCGC 1 cut(s) 494
Csp6I GTAC 1 cut(s) 160
CviAII CATG 2 cut(s) 46, 197
CviQI GTAC 1 cut(s) 160
DdeI CTNAG 1 cut(s) 90
DpnI GATC 4 cut(s) 38, 308, 364, 416
DpnII GATC 4 cut(s) 36, 306, 362, 414
Eco130I CCWWGG 1 cut(s) 45
Eco31I GGTCTC 1 cut(s) 129
Eco32I GATATC 1 cut(s) 384
Eco72I CACGTG 1 cut(s) 303
Eco88I CYCGRG 1 cut(s) 139
EcoRI GAATTC 1 cut(s) 63
EcoRV GATATC 1 cut(s) 384
EcoT14I CCWWGG 1 cut(s) 45
ErhI CCWWGG 1 cut(s) 45
FaeI CATG 2 cut(s) 49, 200
FaiI YATR 4 cut(s) 47, 198, 311, 407
FaqI GGGAC 1 cut(s) 358
FatI CATG 2 cut(s) 45, 196
Fnu4HI GCNGC 1 cut(s) 171
FokI GGATG 2 cut(s) 62, 164
Fsp4HI GCNGC 1 cut(s) 171
FspBI CTAG 1 cut(s) 41
GlaI GCGC 1 cut(s) 493
GluI GCNGC 1 cut(s) 171
HaeIII GGCC 2 cut(s) 435, 498
HhaI GCGC 1 cut(s) 494
Hin1II CATG 2 cut(s) 49, 200
Hin6I GCGC 1 cut(s) 492
HinP1I GCGC 1 cut(s) 492
HphI GGTGA 1 cut(s) 112
Hpy166II GTNNAC 1 cut(s) 430
Hpy188I TCNGA 2 cut(s) 93, 294
Hpy188III TCNNGA 4 cut(s) 34, 240, 360, 366
Hpy8I GTNNAC 1 cut(s) 430
HpyAV CCTTC 1 cut(s) 163
HpyCH4III ACNGT 2 cut(s) 164, 377
HpyCH4IV ACGT 1 cut(s) 302
HpyCH4V TGCA 4 cut(s) 30, 177, 323, 464
HpyF10VI GCNNNNNNNGC 2 cut(s) 86, 500
HpyF3I CTNAG 1 cut(s) 90
HpySE526I ACGT 1 cut(s) 302
Hsp92II CATG 2 cut(s) 49, 200
HspAI GCGC 1 cut(s) 492
Kzo9I GATC 4 cut(s) 36, 306, 362, 414
LpnPI CCDG 2 cut(s) 45, 480
Lsp1109I GCAGC 1 cut(s) 157
LweI GCATC 2 cut(s) 39, 186
MaeI CTAG 1 cut(s) 41
MaeII ACGT 1 cut(s) 302
MaeIII GTNAC 2 cut(s) 143, 199
MalI GATC 4 cut(s) 38, 308, 364, 416
MboI GATC 4 cut(s) 36, 306, 362, 414
MboII GAAGA 2 cut(s) 13, 124
MflI RGATCY 1 cut(s) 414
MluCI AATT 2 cut(s) 63, 208
MnlI CCTC 5 cut(s) 247, 271, 404, 428, 446
MwoI GCNNNNNNNGC 2 cut(s) 86, 500
NcoI CCATGG 1 cut(s) 45
NdeII GATC 4 cut(s) 36, 306, 362, 414
NlaIII CATG 2 cut(s) 49, 200
NlaIV GGNNCC 1 cut(s) 416
NmeAIII GCCGAG 1 cut(s) 48
PkrI GCNGC 1 cut(s) 172
PmaCI CACGTG 1 cut(s) 303
PmlI CACGTG 1 cut(s) 303
Ppu21I YACGTR 1 cut(s) 303
PspCI CACGTG 1 cut(s) 303
PspN4I GGNNCC 1 cut(s) 416
PsuI RGATCY 1 cut(s) 414
RsaI GTAC 1 cut(s) 161
RsaNI GTAC 1 cut(s) 160
SatI GCNGC 1 cut(s) 171
Sau3AI GATC 4 cut(s) 36, 306, 362, 414
SetI ASST 6 cut(s) 91, 102, 172, 252, 279, 305
SfaNI GCATC 2 cut(s) 39, 186
SmlI CTYRAG 2 cut(s) 238, 358
SmoI CTYRAG 2 cut(s) 238, 358
Sse9I AATT 2 cut(s) 63, 208
SspMI CTAG 1 cut(s) 41
StyI CCWWGG 1 cut(s) 45
TaaI ACNGT 2 cut(s) 164, 377
TaiI ACGT 1 cut(s) 305
TaqI TCGA 3 cut(s) 126, 329, 365
TasI AATT 2 cut(s) 63, 208
TseI GCWGC 1 cut(s) 170
TspDTI ATGAA 1 cut(s) 13
XapI RAATTY 1 cut(s) 63
XspI CTAG 1 cut(s) 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.