Rh5CG043500

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0014095
Physical Location & Seq
Reverse (-)
3012419 .. 3013120
702 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG043500.1

Sequence Viewer

Length: 537 bp
ATGGCGAGAAACATCGGTCCTCTCGTGTGTCTCTCGGTCATGATCATGGATGTTATCGCCGGAATACTAGGCATCCAGGCTGAAATAGCACAAAACAAGGTGAAGCATTTGAAGGCGTGGATTATCGAGTGCAGAGACCCGAGTTACCAAGCTTTCAAACTCGGTTTAGCTGCGGCTGTGCTTCTAGCGCTAGCACACACCATTGGGAACTTGCTCGGTGGGTGCATTTGCCTCTGGTCTAAGCAAGACTTCACCAAGGCCACAACAGCTAACAAGAAACTAGCTTTTGGGTCCCTGATTCTCTCATGGATAACTTTAGCACTGGGATTTTCACTGTTAATCATTGGAACAATGGCAAACTCAAAATCAAGAAAATCTTGCTCATTAGCACACGGTCGAGTCCTATCAATAGGAGGAATCATCTGCTTCTTCCACGGATTGTTCACAGTGGCATACTACGTCTCAGCAAAGGCCACAATCAGAGATGAGGAAAAGCGCAGGAACCCTACAAACCAAGCCGGCCATCCTCCGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

178

Amino Acids

19.15

Weight (kDa)

9.7

Isoelectric Point (pI)

37.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 58 - 146 7.2e-19 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014095)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21310 AT4G21310
fragaria_vesca FvH4_3g02950
malus_domestica MD10G1311300.v1.1
prunus_persica Prupe.4G029600_v2.0.a1
pyrus_communis pycom10g26250
rosa_chinensis RchiOBHm_Chr5g0004561
rosa_laevigata RLG00000031240
rosa_multiflora Rmu_sc0002354.1_g000018 Rmu_sc0003758.1_g000012
rosa_roxburghii Rroxscaffold_1G00070970
rosa_rugosa Rorug04G0412200
rosa_samantha Rh5AG040200 Rh5BG039600 Rh5CG043500 Rh5DG039300
rosa_wichuraiana Rw5G003860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 173
AcoI YGGCCR 1 cut(s) 520
AfeI AGCGCT 1 cut(s) 189
AfiI CCNNNNNNNGG 1 cut(s) 529
AgsI TTSAA 2 cut(s) 112, 157
AjnI CCWGG 1 cut(s) 75
AluBI AGCT 4 cut(s) 152, 170, 269, 284
AluI AGCT 4 cut(s) 152, 170, 269, 284
Alw26I GTCTC 3 cut(s) 35, 129, 466
Ama87I CYCGRG 1 cut(s) 139
Aor51HI AGCGCT 1 cut(s) 189
AoxI GGCC 3 cut(s) 258, 471, 520
ApeKI GCWGC 1 cut(s) 170
AspLEI GCGC 2 cut(s) 190, 498
AspS9I GGNCC 2 cut(s) 17, 291
AsuHPI GGTGA 2 cut(s) 112, 244
AsuNHI GCTAGC 1 cut(s) 190
AvaI CYCGRG 1 cut(s) 139
AvaII GGWCC 2 cut(s) 17, 291
BauI CACGAG 1 cut(s) 23
BbvI GCAGC 1 cut(s) 157
BccI CCATC 1 cut(s) 531
BciT130I CCWGG 1 cut(s) 77
BclI TGATCA 1 cut(s) 42
BcoDI GTCTC 3 cut(s) 35, 129, 466
BfaI CTAG 4 cut(s) 68, 185, 191, 281
BfoI RGCGCY 1 cut(s) 191
BisI GCNGC 2 cut(s) 171, 174
BlsI GCNGC 2 cut(s) 172, 175
Bme1390I CCNGG 1 cut(s) 77
Bme18I GGWCC 2 cut(s) 17, 291
BmeT110I CYCGRG 1 cut(s) 139
BmgT120I GGNCC 2 cut(s) 17, 291
BmiI GGNNCC 3 cut(s) 292, 293, 503
BmrFI CCNGG 1 cut(s) 77
BmrI ACTGGG 1 cut(s) 332
BmsI GCATC 1 cut(s) 81
BmtI GCTAGC 1 cut(s) 194
BmuI ACTGGG 1 cut(s) 332
BsaI GGTCTC 1 cut(s) 129
BsaJI CCNNGG 2 cut(s) 255, 433
Bsc4I CCNNNNNNNGG 1 cut(s) 529
Bse118I RCCGGY 1 cut(s) 518
Bse1I ACTGG 1 cut(s) 327
BseBI CCWGG 1 cut(s) 77
BseDI CCNNGG 2 cut(s) 255, 433
BseGI GGATG 3 cut(s) 55, 72, 523
BseLI CCNNNNNNNGG 1 cut(s) 529
BseMII CTCAG 1 cut(s) 477
BseNI ACTGG 1 cut(s) 327
BseXI GCAGC 1 cut(s) 157
BsgI GTGCAG 1 cut(s) 151
Bsh1285I CGRYCG 1 cut(s) 397
BshFI GGCC 3 cut(s) 260, 473, 522
BsiEI CGRYCG 1 cut(s) 397
BsiHKCI CYCGRG 1 cut(s) 139
BsiSI CCGG 3 cut(s) 60, 519, 530
BslFI GGGAC 1 cut(s) 277
BslI CCNNNNNNNGG 1 cut(s) 529
BsmAI GTCTC 3 cut(s) 35, 129, 466
BsmBI CGTCTC 1 cut(s) 466
BsmFI GGGAC 1 cut(s) 277
BsnI GGCC 3 cut(s) 260, 473, 522
Bso31I GGTCTC 1 cut(s) 129
BsoBI CYCGRG 1 cut(s) 139
Bsp143I GATC 1 cut(s) 42
BspACI CCGC 1 cut(s) 173
BspANI GGCC 3 cut(s) 260, 473, 522
BspCNI CTCAG 1 cut(s) 476
BspHI TCATGA 1 cut(s) 39
BspLI GGNNCC 3 cut(s) 292, 293, 503
BspOI GCTAGC 1 cut(s) 194
BspTNI GGTCTC 1 cut(s) 129
BsrFI RCCGGY 1 cut(s) 518
BsrI ACTGG 1 cut(s) 327
BssAI RCCGGY 1 cut(s) 518
BssECI CCNNGG 2 cut(s) 255, 433
BssMI GATC 1 cut(s) 42
BssSI CACGAG 1 cut(s) 23
BssT1I CCWWGG 1 cut(s) 255
Bst2BI CACGAG 1 cut(s) 23
Bst2UI CCWGG 1 cut(s) 77
Bst4CI ACNGT 3 cut(s) 336, 395, 448
BstC8I GCNNGC 2 cut(s) 192, 520
BstDEI CTNAG 3 cut(s) 240, 463, 534
BstDSI CCRYGG 1 cut(s) 433
BstF5I GGATG 3 cut(s) 55, 72, 523
BstH2I RGCGCY 1 cut(s) 191
BstHHI GCGC 2 cut(s) 190, 498
BstKTI GATC 1 cut(s) 45
BstMAI GTCTC 3 cut(s) 35, 129, 466
BstMBI GATC 1 cut(s) 42
BstMCI CGRYCG 1 cut(s) 397
BstMWI GCNNNNNNNGC 3 cut(s) 86, 187, 266
BstNI CCWGG 1 cut(s) 77
BstSCI CCNGG 1 cut(s) 75
BstV1I GCAGC 1 cut(s) 157
BsuRI GGCC 3 cut(s) 260, 473, 522
BtgI CCRYGG 1 cut(s) 433
BtsCI GGATG 3 cut(s) 55, 72, 523
BtsIMutI CAGTG 3 cut(s) 320, 332, 453
Cac8I GCNNGC 2 cut(s) 192, 520
CciI TCATGA 1 cut(s) 39
CfoI GCGC 2 cut(s) 190, 498
Cfr10I RCCGGY 1 cut(s) 518
Cfr13I GGNCC 2 cut(s) 17, 291
CviAII CATG 3 cut(s) 40, 46, 306
DdeI CTNAG 3 cut(s) 240, 463, 534
DpnI GATC 1 cut(s) 44
DpnII GATC 1 cut(s) 42
EaeI YGGCCR 1 cut(s) 520
Eco130I CCWWGG 1 cut(s) 255
Eco31I GGTCTC 1 cut(s) 129
Eco47I GGWCC 2 cut(s) 17, 291
Eco47III AGCGCT 1 cut(s) 189
Eco88I CYCGRG 1 cut(s) 139
EcoO109I RGGNCCY 1 cut(s) 291
EcoRII CCWGG 1 cut(s) 75
EcoT14I CCWWGG 1 cut(s) 255
ErhI CCWWGG 1 cut(s) 255
Esp3I CGTCTC 1 cut(s) 466
FaeI CATG 3 cut(s) 43, 49, 309
FaiI YATR 4 cut(s) 41, 47, 307, 454
FalI AAGNNNNNCTT 4 cut(s) 233, 265, 361, 393
FaqI GGGAC 1 cut(s) 277
FatI CATG 3 cut(s) 39, 45, 305
FbaI TGATCA 1 cut(s) 42
Fnu4HI GCNGC 2 cut(s) 171, 174
FokI GGATG 3 cut(s) 59, 62, 510
Fsp4HI GCNGC 2 cut(s) 171, 174
FspBI CTAG 4 cut(s) 68, 185, 191, 281
GlaI GCGC 2 cut(s) 189, 497
GluI GCNGC 2 cut(s) 171, 174
HaeII RGCGCY 1 cut(s) 191
HaeIII GGCC 3 cut(s) 260, 473, 522
HapII CCGG 3 cut(s) 60, 519, 530
HhaI GCGC 2 cut(s) 190, 498
Hin1II CATG 3 cut(s) 43, 49, 309
Hin6I GCGC 2 cut(s) 188, 496
HinP1I GCGC 2 cut(s) 188, 496
HindIII AAGCTT 1 cut(s) 150
HinfI GANTC 3 cut(s) 298, 399, 417
HpaII CCGG 3 cut(s) 60, 519, 530
HphI GGTGA 2 cut(s) 112, 244
Hpy166II GTNNAC 1 cut(s) 444
Hpy188I TCNGA 1 cut(s) 482
Hpy188III TCNNGA 2 cut(s) 40, 369
Hpy8I GTNNAC 1 cut(s) 444
HpyAV CCTTC 1 cut(s) 106
HpyCH4III ACNGT 3 cut(s) 336, 395, 448
HpyCH4IV ACGT 1 cut(s) 459
HpyCH4V TGCA 2 cut(s) 132, 225
HpyF10VI GCNNNNNNNGC 3 cut(s) 86, 187, 266
HpyF3I CTNAG 3 cut(s) 240, 463, 534
HpySE526I ACGT 1 cut(s) 459
Hsp92II CATG 3 cut(s) 43, 49, 309
HspAI GCGC 2 cut(s) 188, 496
KflI GGGWCCC 1 cut(s) 291
KroI GCCGGC 1 cut(s) 518
KroNI GCCGGC 1 cut(s) 520
Ksp22I TGATCA 1 cut(s) 42
Kzo9I GATC 1 cut(s) 42
LpnPI CCDG 8 cut(s) 62, 73, 89, 220, 308, 308, 484, 532
Lsp1109I GCAGC 1 cut(s) 157
LweI GCATC 1 cut(s) 81
MaeI CTAG 4 cut(s) 68, 185, 191, 281
MaeII ACGT 1 cut(s) 459
MaeIII GTNAC 1 cut(s) 143
MalI GATC 1 cut(s) 44
MboI GATC 1 cut(s) 42
MboII GAAGA 1 cut(s) 421
MlyI GAGTC 1 cut(s) 408
MnlI CCTC 5 cut(s) 30, 242, 407, 481, 537
MroNI GCCGGC 1 cut(s) 518
MseI TTAA 1 cut(s) 338
MslI CAYNNNNRTG 1 cut(s) 44
MspI CCGG 3 cut(s) 60, 519, 530
MspR9I CCNGG 1 cut(s) 77
MvaI CCWGG 1 cut(s) 77
MwoI GCNNNNNNNGC 3 cut(s) 86, 187, 266
NaeI GCCGGC 1 cut(s) 520
NdeII GATC 1 cut(s) 42
NgoMIV GCCGGC 1 cut(s) 518
NheI GCTAGC 1 cut(s) 190
NlaIII CATG 3 cut(s) 43, 49, 309
NlaIV GGNNCC 3 cut(s) 292, 293, 503
PagI TCATGA 1 cut(s) 39
PcsI WCGNNNNNNNCGW 1 cut(s) 21
PdiI GCCGGC 1 cut(s) 520
PfeI GAWTC 2 cut(s) 298, 417
PkrI GCNGC 2 cut(s) 172, 175
PleI GAGTC 1 cut(s) 407
PpsI GAGTC 1 cut(s) 407
PpuMI RGGWCCY 1 cut(s) 291
Psp5II RGGWCCY 1 cut(s) 291
Psp6I CCWGG 1 cut(s) 75
PspGI CCWGG 1 cut(s) 75
PspN4I GGNNCC 3 cut(s) 292, 293, 503
PspPI GGNCC 2 cut(s) 17, 291
PspPPI RGGWCCY 1 cut(s) 291
RseI CAYNNNNRTG 1 cut(s) 44
SaqAI TTAA 1 cut(s) 338
SatI GCNGC 2 cut(s) 171, 174
Sau3AI GATC 1 cut(s) 42
Sau96I GGNCC 2 cut(s) 17, 291
SchI GAGTC 1 cut(s) 408
ScrFI CCNGG 1 cut(s) 77
SetI ASST 6 cut(s) 102, 154, 172, 271, 286, 462
SfaNI GCATC 1 cut(s) 81
SinI GGWCC 2 cut(s) 17, 291
SmiMI CAYNNNNRTG 1 cut(s) 44
SsiI CCGC 1 cut(s) 173
SspMI CTAG 4 cut(s) 68, 185, 191, 281
StyD4I CCNGG 1 cut(s) 75
StyI CCWWGG 1 cut(s) 255
TaaI ACNGT 3 cut(s) 336, 395, 448
TaiI ACGT 1 cut(s) 462
TaqI TCGA 2 cut(s) 126, 397
TaqII GACCGA 2 cut(s) 5, 25
TauI GCSGC 1 cut(s) 176
TfiI GAWTC 2 cut(s) 298, 417
Tru1I TTAA 1 cut(s) 338
Tru9I TTAA 1 cut(s) 338
TscAI CASTG 3 cut(s) 327, 339, 453
TseI GCWGC 1 cut(s) 170
TspGWI ACGGA 1 cut(s) 450
TspRI CASTG 3 cut(s) 327, 339, 453
VpaK11BI GGWCC 2 cut(s) 17, 291
XspI CTAG 4 cut(s) 68, 185, 191, 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.