FvH4_3g02950

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0014095
Physical Location & Seq
Reverse (-)
1569878 .. 1570816
939 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g02950.t1

Sequence Viewer

Length: 570 bp
ATGGCCACTTCTTCTTCTTCAGCCAACGCTTCGAAAATGGCAAGGAACATCGGCCCTCTCGTGTGCCTCTTGGTCATGATCATGGATGCTATTGCCGGAATACTTGGCATTCAGGCTGAAGTATCCCAAAACAAGGTGAAGCATTTGAAGGTGTGGATTTTCGAGTGCAGAGACCCGAGTTACCAGGCTTTCAAACTCGGTTTGGCTGCGGCTGTGCTTCTGGCGCTAGCGCACACCATTGGGAACTTGCTTGGTGGGTGCGTTTGCCTCTGGTCTAAGCAAAACTACGACAACGCCACAGCTAACAAAAGACTAGCTTTTGGATCCCTCATTCTCTCATGGATAAATTTCGCAATCGGATTCTCATTGTTAATGGCCGGAGCAATGGCAAACTCAAAATCAAGAAAATCTTGCGGATTATCAAACCATAGGATCCTATCAATTGGAGGCATCCTCTGCTTCTTTCATGGATTGTTCACACTAGCCTACTACGTCTCGGCAAAGACCACAATCCGAGACGAAGAAAAGCTCAAGAACCCTACCAACCACGGCGGCCATCCTCCGGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

190

Amino Acids

20.28

Weight (kDa)

9.56

Isoelectric Point (pI)

37.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 70 - 157 2.3e-20 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014095)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21310 AT4G21310
fragaria_vesca FvH4_3g02950
malus_domestica MD10G1311300.v1.1
prunus_persica Prupe.4G029600_v2.0.a1
pyrus_communis pycom10g26250
rosa_chinensis RchiOBHm_Chr5g0004561
rosa_laevigata RLG00000031240
rosa_multiflora Rmu_sc0002354.1_g000018 Rmu_sc0003758.1_g000012
rosa_roxburghii Rroxscaffold_1G00070970
rosa_rugosa Rorug04G0412200
rosa_samantha Rh5AG040200 Rh5BG039600 Rh5CG043500 Rh5DG039300
rosa_wichuraiana Rw5G003860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 209, 414, 552
AclWI GGATC 4 cut(s) 318, 331, 427, 440
AcoI YGGCCR 3 cut(s) 3, 375, 553
AcsI RAATTY 1 cut(s) 346
AcuI CTGAAG 1 cut(s) 138
AfiI CCNNNNNNNGG 2 cut(s) 133, 562
AgsI TTSAA 2 cut(s) 148, 193
AjnI CCWGG 1 cut(s) 183
AluBI AGCT 3 cut(s) 302, 317, 529
AluI AGCT 3 cut(s) 302, 317, 529
Alw26I GTCTC 3 cut(s) 165, 499, 510
AlwI GGATC 4 cut(s) 318, 331, 427, 440
Ama87I CYCGRG 1 cut(s) 175
AoxI GGCC 4 cut(s) 3, 52, 375, 553
ApeKI GCWGC 1 cut(s) 206
ApoI RAATTY 1 cut(s) 346
AspLEI GCGC 2 cut(s) 226, 232
AspS9I GGNCC 1 cut(s) 53
AsuHPI GGTGA 1 cut(s) 148
AsuII TTCGAA 1 cut(s) 32
AsuNHI GCTAGC 1 cut(s) 226
AvaI CYCGRG 1 cut(s) 175
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 2 cut(s) 323, 432
BauI CACGAG 1 cut(s) 59
BbvI GCAGC 1 cut(s) 193
BccI CCATC 1 cut(s) 564
BceAI ACGGC 1 cut(s) 565
BciT130I CCWGG 1 cut(s) 185
BciVI GTATCC 1 cut(s) 133
BclI TGATCA 1 cut(s) 78
BcoDI GTCTC 3 cut(s) 165, 499, 510
BfaI CTAG 3 cut(s) 227, 314, 482
BfoI RGCGCY 1 cut(s) 227
BfuI GTATCC 1 cut(s) 133
BisI GCNGC 3 cut(s) 207, 210, 553
BlsI GCNGC 3 cut(s) 208, 211, 554
Bme1390I CCNGG 1 cut(s) 185
BmeT110I CYCGRG 1 cut(s) 175
BmgT120I GGNCC 1 cut(s) 53
BmiI GGNNCC 2 cut(s) 325, 434
BmrFI CCNGG 1 cut(s) 185
BmsI GCATC 2 cut(s) 76, 459
BmtI GCTAGC 1 cut(s) 230
BplI GAGNNNNNCTC 2 cut(s) 438, 470
Bpu14I TTCGAA 1 cut(s) 32
BpuEI CTTGAG 1 cut(s) 515
BsaI GGTCTC 1 cut(s) 165
BsaJI CCNNGG 1 cut(s) 547
Bsc4I CCNNNNNNNGG 2 cut(s) 133, 562
Bse3DI GCAATG 1 cut(s) 390
BseBI CCWGG 1 cut(s) 185
BseDI CCNNGG 1 cut(s) 547
BseGI GGATG 3 cut(s) 91, 450, 556
BseLI CCNNNNNNNGG 2 cut(s) 133, 562
BseMI GCAATG 1 cut(s) 390
BseXI GCAGC 1 cut(s) 193
BsgI GTGCAG 1 cut(s) 187
BshFI GGCC 4 cut(s) 5, 54, 377, 555
BsiHKCI CYCGRG 1 cut(s) 175
BsiSI CCGG 3 cut(s) 96, 378, 563
BslI CCNNNNNNNGG 2 cut(s) 133, 562
BsmAI GTCTC 3 cut(s) 165, 499, 510
BsmBI CGTCTC 2 cut(s) 499, 510
BsmI GAATGC 1 cut(s) 108
BsnI GGCC 4 cut(s) 5, 54, 377, 555
Bso31I GGTCTC 1 cut(s) 165
BsoBI CYCGRG 1 cut(s) 175
Bsp119I TTCGAA 1 cut(s) 32
Bsp143I GATC 3 cut(s) 78, 323, 432
BspACI CCGC 3 cut(s) 209, 414, 552
BspANI GGCC 4 cut(s) 5, 54, 377, 555
BspHI TCATGA 1 cut(s) 75
BspLI GGNNCC 2 cut(s) 325, 434
BspOI GCTAGC 1 cut(s) 230
BspPI GGATC 4 cut(s) 318, 331, 427, 440
BspT104I TTCGAA 1 cut(s) 32
BspTNI GGTCTC 1 cut(s) 165
BsrDI GCAATG 1 cut(s) 390
BssECI CCNNGG 1 cut(s) 547
BssMI GATC 3 cut(s) 78, 323, 432
BssSI CACGAG 1 cut(s) 59
Bst2BI CACGAG 1 cut(s) 59
Bst2UI CCWGG 1 cut(s) 185
BstAPI GCANNNNNTGC 1 cut(s) 456
BstBI TTCGAA 1 cut(s) 32
BstC8I GCNNGC 1 cut(s) 228
BstDEI CTNAG 1 cut(s) 276
BstDSI CCRYGG 1 cut(s) 547
BstF5I GGATG 3 cut(s) 91, 450, 556
BstH2I RGCGCY 1 cut(s) 227
BstHHI GCGC 2 cut(s) 226, 232
BstKTI GATC 3 cut(s) 81, 326, 435
BstMAI GTCTC 3 cut(s) 165, 499, 510
BstMBI GATC 3 cut(s) 78, 323, 432
BstMWI GCNNNNNNNGC 2 cut(s) 223, 456
BstNI CCWGG 1 cut(s) 185
BstSCI CCNGG 1 cut(s) 183
BstV1I GCAGC 1 cut(s) 193
BstX2I RGATCY 2 cut(s) 323, 432
BstYI RGATCY 2 cut(s) 323, 432
BsuI GTATCC 1 cut(s) 133
BsuRI GGCC 4 cut(s) 5, 54, 377, 555
BtgI CCRYGG 1 cut(s) 547
BtsCI GGATG 3 cut(s) 91, 450, 556
Cac8I GCNNGC 1 cut(s) 228
CciI TCATGA 1 cut(s) 75
CfoI GCGC 2 cut(s) 226, 232
Cfr13I GGNCC 1 cut(s) 53
CviAII CATG 4 cut(s) 76, 82, 339, 467
DdeI CTNAG 1 cut(s) 276
DpnI GATC 3 cut(s) 80, 325, 434
DpnII GATC 3 cut(s) 78, 323, 432
EaeI YGGCCR 3 cut(s) 3, 375, 553
Eco31I GGTCTC 1 cut(s) 165
Eco57I CTGAAG 1 cut(s) 138
Eco88I CYCGRG 1 cut(s) 175
EcoRII CCWGG 1 cut(s) 183
Esp3I CGTCTC 2 cut(s) 499, 510
FaeI CATG 4 cut(s) 79, 85, 342, 470
FaiI YATR 6 cut(s) 77, 83, 340, 429, 468, 568
FalI AAGNNNNNCTT 4 cut(s) 301, 333, 394, 426
FatI CATG 4 cut(s) 75, 81, 338, 466
FbaI TGATCA 1 cut(s) 78
Fnu4HI GCNGC 3 cut(s) 207, 210, 553
FokI GGATG 3 cut(s) 98, 437, 543
Fsp4HI GCNGC 3 cut(s) 207, 210, 553
FspBI CTAG 3 cut(s) 227, 314, 482
GlaI GCGC 2 cut(s) 225, 231
GluI GCNGC 3 cut(s) 207, 210, 553
HaeII RGCGCY 1 cut(s) 227
HaeIII GGCC 4 cut(s) 5, 54, 377, 555
HapII CCGG 3 cut(s) 96, 378, 563
HhaI GCGC 2 cut(s) 226, 232
Hin1II CATG 4 cut(s) 79, 85, 342, 470
Hin6I GCGC 2 cut(s) 224, 230
HinP1I GCGC 2 cut(s) 224, 230
HinfI GANTC 1 cut(s) 360
HpaII CCGG 3 cut(s) 96, 378, 563
HphI GGTGA 1 cut(s) 148
Hpy166II GTNNAC 1 cut(s) 477
Hpy188I TCNGA 2 cut(s) 359, 515
Hpy188III TCNNGA 3 cut(s) 76, 402, 532
Hpy8I GTNNAC 1 cut(s) 477
HpyAV CCTTC 1 cut(s) 142
HpyCH4IV ACGT 1 cut(s) 492
HpyCH4V TGCA 1 cut(s) 168
HpyF10VI GCNNNNNNNGC 2 cut(s) 223, 456
HpyF3I CTNAG 1 cut(s) 276
HpySE526I ACGT 1 cut(s) 492
Hsp92II CATG 4 cut(s) 79, 85, 342, 470
HspAI GCGC 2 cut(s) 224, 230
Ksp22I TGATCA 1 cut(s) 78
Kzo9I GATC 3 cut(s) 78, 323, 432
LmnI GCTCC 1 cut(s) 380
LpnPI CCDG 7 cut(s) 98, 109, 170, 197, 206, 256, 391
Lsp1109I GCAGC 1 cut(s) 193
LweI GCATC 2 cut(s) 76, 459
MaeI CTAG 3 cut(s) 227, 314, 482
MaeII ACGT 1 cut(s) 492
MaeIII GTNAC 1 cut(s) 179
MalI GATC 3 cut(s) 80, 325, 434
MboI GATC 3 cut(s) 78, 323, 432
MboII GAAGA 4 cut(s) 3, 6, 9, 533
MfeI CAATTG 1 cut(s) 441
MflI RGATCY 2 cut(s) 323, 432
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 346, 441
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 7 cut(s) 66, 77, 278, 338, 440, 464, 570
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 371
MslI CAYNNNNRTG 1 cut(s) 80
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 3 cut(s) 96, 378, 563
MspR9I CCNGG 1 cut(s) 185
MunI CAATTG 1 cut(s) 441
Mva1269I GAATGC 1 cut(s) 108
MvaI CCWGG 1 cut(s) 185
MwoI GCNNNNNNNGC 2 cut(s) 223, 456
NdeII GATC 3 cut(s) 78, 323, 432
NheI GCTAGC 1 cut(s) 226
NlaIII CATG 4 cut(s) 79, 85, 342, 470
NlaIV GGNNCC 2 cut(s) 325, 434
NmeAIII GCCGAG 1 cut(s) 476
NspV TTCGAA 1 cut(s) 32
PagI TCATGA 1 cut(s) 75
PcsI WCGNNNNNNNCGW 1 cut(s) 57
PctI GAATGC 1 cut(s) 108
PfeI GAWTC 1 cut(s) 360
PkrI GCNGC 3 cut(s) 208, 211, 554
Psp6I CCWGG 1 cut(s) 183
PspGI CCWGG 1 cut(s) 183
PspN4I GGNNCC 2 cut(s) 325, 434
PspPI GGNCC 1 cut(s) 53
PsuI RGATCY 2 cut(s) 323, 432
RseI CAYNNNNRTG 1 cut(s) 80
SaqAI TTAA 1 cut(s) 371
SatI GCNGC 3 cut(s) 207, 210, 553
Sau3AI GATC 3 cut(s) 78, 323, 432
Sau96I GGNCC 1 cut(s) 53
ScrFI CCNGG 1 cut(s) 185
SetI ASST 6 cut(s) 138, 153, 304, 319, 495, 531
SfaNI GCATC 2 cut(s) 76, 459
SfuI TTCGAA 1 cut(s) 32
SmiMI CAYNNNNRTG 1 cut(s) 80
SmlI CTYRAG 1 cut(s) 530
SmoI CTYRAG 1 cut(s) 530
Sse9I AATT 2 cut(s) 346, 441
SsiI CCGC 3 cut(s) 209, 414, 552
SspMI CTAG 3 cut(s) 227, 314, 482
StyD4I CCNGG 1 cut(s) 183
TaiI ACGT 1 cut(s) 495
TaqI TCGA 2 cut(s) 32, 162
TasI AATT 2 cut(s) 346, 441
TauI GCSGC 2 cut(s) 212, 555
TfiI GAWTC 1 cut(s) 360
Tru1I TTAA 1 cut(s) 371
Tru9I TTAA 1 cut(s) 371
TseI GCWGC 1 cut(s) 206
TspDTI ATGAA 1 cut(s) 455
XapI RAATTY 1 cut(s) 346
XspI CTAG 3 cut(s) 227, 314, 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.