AT4G22290

membrane protein At1g16860-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0009605
Physical Location & Seq
Reverse (-)
11782840 .. 11785937
3098 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G22290.1

Sequence Viewer

Length: 1338 bp
ATGGCGGGTCGGATCCAATCCCACCAGCTACCAAATGGACTCTACGTATCGGGAAAGCTTGAGCAACCAAAAGAGCGACCACCGACAATGGCGGCTCGAGCTGTGCCTTACACCGGAGGCGACATCAAGAAATCCGGCGAGCTCGGAAGGATGTTCGACATCTCCGTCGTCGATTCAGCTTCTTTTCAAGGACCACCGCCGTTAATCGTCGGTGGTAATAGCAGCGGAGGTACGTCAAGGCTTCAGGCGCCGCCACGTGTCTCCGGTTCTTCTTCAAACCCTAACAGTGGATCCGTTCGATCTGGTCCCAACTCTGGTTCGGTTAAGAAATTCTCAGGTCCACTCTCTCAGCTTCAGCCAACCGGTTTAATTACCTCCGGTTCTCTTGGTTCTTCGGGTCCAATTTTGTCCGGGTCTAGAAGGTCGGGTCAATTGGATCACCAATTGAGTAATCTAGCGTCGAGCAAGCCTAAATACGGGTCGTCGGTGACGAGTCTTAACGTAGACCCGGTTCGGGTCGGGTTTAAAGTACCAAAAGCAATGGTTTGGGCGGTTTTGATAGTGGCGGCGATGGGATTGCTTGTGGGAGCTTTTCTTACTGTGGCGGTGAAGAAACCGGTGGTGATTGCTGCGGTTTTAGCAGCAGTTTGTCCTGCAATCGTGGTTTTGGTTTGGAATTGCGTTTGGAGAAGAAAAGGGTTGTTGAGTTTTATCAAAAAGTATCCAGATGCTGAGCTTAGAGGTGCCATTGATGGACAATTCGTTAAGGTTACAGGGGTTGTGACATGTGGAAGCATTCCTTTGGAATCTTCGTTCCAAAGAACACCAAGATGCGTTTATGTATCCACGGAGTTGTATGAATACAAAGGTTTTGGTGGGAAATCCGCAAACCCAAAACATCGATGCTTTTCTTGGGGATCTAGACATGCAGAGAAATATGTGTCGGATTTTTACATCTCGGATTTCCAATCTGGATTGCGAGCACTAGTAAAAGCAGGATATGGATCAAAAGTTTCTCCTTTTGTCAAACCGGCGACAGTGGCTAATGTAACAACGCAGAACAAAGATTTATCTCCCAGCTTCTTAAAGTGGCTGTCAGATCGCAACCTCTCAGCTGATGACCGTGTTATGCGTCTCAAAGAAGGATACATAAAAGAAGGAAGCACAGTGAGCGTGATGGGGATGGTAAGAAGGCACGACAACGTTCTGATGATAGTTCCTCCGGCAGAAGCAGTCTCCAGTGGGTGCCGGTGGTGGCACTGCCTCTTCCCAACCTACGCAGATGGCTTAATCATAACCTGCGACGATAATCAAAACGCTGATGTCATTCCTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

445

Amino Acids

47.54

Weight (kDa)

9.75

Isoelectric Point (pI)

34.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 164
Acc36I ACCTGC 1 cut(s) 1307
AccB1I GGYRCC 3 cut(s) 247, 743, 1245
AccI GTMKAC 1 cut(s) 504
AclI AACGTT 1 cut(s) 1203
AclWI GGATC 7 cut(s) 7, 20, 285, 298, 444, 925, 1012
AcsI RAATTY 1 cut(s) 329
AcuI CTGAAG 2 cut(s) 227, 338
AcvI CACGTG 1 cut(s) 257
AcyI GRCGYC 1 cut(s) 248
AfaI GTAC 2 cut(s) 232, 531
AfiI CCNNNNNNNGG 5 cut(s) 113, 287, 314, 476, 514
AflIII ACRYGT 2 cut(s) 256, 785
AgeI ACCGGT 2 cut(s) 362, 616
AgsI TTSAA 2 cut(s) 188, 276
AhlI ACTAGT 1 cut(s) 985
AloI GAACNNNNNNTCC 2 cut(s) 797, 829
Alw21I GWGCWC 2 cut(s) 144, 985
Alw26I GTCTC 3 cut(s) 265, 1139, 1240
AlwI GGATC 7 cut(s) 7, 20, 285, 298, 444, 925, 1012
AlwNI CAGNNNCTG 1 cut(s) 731
Ama87I CYCGRG 1 cut(s) 96
ApeKI GCWGC 3 cut(s) 222, 629, 641
ApoI RAATTY 1 cut(s) 329
AsiGI ACCGGT 2 cut(s) 362, 616
AspLEI GCGC 1 cut(s) 250
AspS9I GGNCC 4 cut(s) 191, 305, 338, 398
AsuC2I CCSGG 2 cut(s) 412, 509
AsuHPI GGTGA 4 cut(s) 431, 499, 619, 634
AvaI CYCGRG 1 cut(s) 96
AvaII GGWCC 4 cut(s) 191, 305, 338, 398
BamHI GGATCC 2 cut(s) 12, 290
BanI GGYRCC 3 cut(s) 247, 743, 1245
BanII GRGCYC 1 cut(s) 144
BbrPI CACGTG 1 cut(s) 257
Bbv12I GWGCWC 2 cut(s) 144, 985
BbvI GCAGC 3 cut(s) 234, 616, 653
BccI CCATC 5 cut(s) 565, 746, 1171, 1177, 1277
BceAI ACGGC 1 cut(s) 184
BcgI CGANNNNNNTGC 2 cut(s) 559, 593
BciVI GTATCC 3 cut(s) 732, 853, 1139
BcnI CCSGG 2 cut(s) 412, 509
BcoDI GTCTC 3 cut(s) 265, 1139, 1240
BcuI ACTAGT 1 cut(s) 985
BfaI CTAG 4 cut(s) 417, 455, 921, 986
BfoI RGCGCY 1 cut(s) 251
BfuAI ACCTGC 1 cut(s) 1307
BfuI GTATCC 3 cut(s) 732, 853, 1139
BisI GCNGC 6 cut(s) 93, 223, 251, 567, 630, 642
BlpI GCTNAGC 1 cut(s) 732
BlsI GCNGC 6 cut(s) 94, 224, 252, 568, 631, 643
Bme1390I CCNGG 2 cut(s) 412, 509
Bme18I GGWCC 4 cut(s) 191, 305, 338, 398
BmeT110I CYCGRG 1 cut(s) 96
BmgT120I GGNCC 4 cut(s) 191, 305, 338, 398
BmiI GGNNCC 7 cut(s) 14, 249, 292, 307, 399, 745, 1247
BmrFI CCNGG 2 cut(s) 412, 509
BmsI GCATC 3 cut(s) 718, 821, 893
BpmI CTGGAG 1 cut(s) 1222
Bpu1102I GCTNAGC 1 cut(s) 732
BpuEI CTTGAG 1 cut(s) 80
BpuMI CCSGG 2 cut(s) 412, 509
Bsa29I ATCGAT 1 cut(s) 901
BsaAI YACGTR 2 cut(s) 46, 257
BsaBI GATNNNNATC 1 cut(s) 1003
BsaHI GRCGYC 1 cut(s) 248
BsaJI CCNNGG 1 cut(s) 846
BsaWI WCCGGW 5 cut(s) 113, 263, 362, 377, 616
Bsc4I CCNNNNNNNGG 5 cut(s) 113, 287, 314, 476, 514
Bse118I RCCGGY 4 cut(s) 362, 616, 1030, 1248
Bse1I ACTGG 1 cut(s) 1239
Bse3DI GCAATG 1 cut(s) 546
Bse8I GATNNNNATC 1 cut(s) 1003
BseCI ATCGAT 1 cut(s) 901
BseDI CCNNGG 1 cut(s) 846
BseGI GGATG 2 cut(s) 156, 1188
BseJI GATNNNNATC 1 cut(s) 1003
BseLI CCNNNNNNNGG 5 cut(s) 113, 287, 314, 476, 514
BseMI GCAATG 1 cut(s) 546
BseMII CTCAG 4 cut(s) 348, 362, 723, 1125
BseNI ACTGG 1 cut(s) 1239
BseXI GCAGC 3 cut(s) 234, 616, 653
BseYI CCCAGC 1 cut(s) 1076
BshNI GGYRCC 3 cut(s) 247, 743, 1245
BshTI ACCGGT 2 cut(s) 362, 616
BshVI ATCGAT 1 cut(s) 901
BsiHKAI GWGCWC 2 cut(s) 144, 985
BsiHKCI CYCGRG 1 cut(s) 96
BslFI GGGAC 1 cut(s) 291
BslI CCNNNNNNNGG 5 cut(s) 113, 287, 314, 476, 514
BsmAI GTCTC 3 cut(s) 265, 1139, 1240
BsmBI CGTCTC 1 cut(s) 1139
BsmFI GGGAC 1 cut(s) 291
BsmI GAATGC 1 cut(s) 795
BsoBI CYCGRG 1 cut(s) 96
Bsp1286I GDGCHC 2 cut(s) 144, 985
Bsp143I GATC 7 cut(s) 12, 290, 299, 436, 917, 1004, 1099
Bsp1720I GCTNAGC 1 cut(s) 732
BspCNI CTCAG 4 cut(s) 347, 361, 724, 1124
BspDI ATCGAT 1 cut(s) 901
BspLI GGNNCC 7 cut(s) 14, 249, 292, 307, 399, 745, 1247
BspMI ACCTGC 1 cut(s) 1307
BspPI GGATC 7 cut(s) 7, 20, 285, 298, 444, 925, 1012
BspT107I GGYRCC 3 cut(s) 247, 743, 1245
BsrDI GCAATG 1 cut(s) 546
BsrFI RCCGGY 4 cut(s) 362, 616, 1030, 1248
BsrI ACTGG 1 cut(s) 1239
BssAI RCCGGY 4 cut(s) 362, 616, 1030, 1248
BssECI CCNNGG 1 cut(s) 846
BssMI GATC 7 cut(s) 12, 290, 299, 436, 917, 1004, 1099
BssNI GRCGYC 1 cut(s) 248
Bst4CI ACNGT 5 cut(s) 287, 601, 1039, 1124, 1168
Bst6I CTCTTC 1 cut(s) 1271
BstACI GRCGYC 1 cut(s) 248
BstBAI YACGTR 2 cut(s) 46, 257
BstC8I GCNNGC 3 cut(s) 140, 467, 981
BstDEI CTNAG 5 cut(s) 334, 348, 732, 737, 1111
BstDSI CCRYGG 1 cut(s) 846
BstF5I GGATG 2 cut(s) 156, 1188
BstH2I RGCGCY 1 cut(s) 251
BstHHI GCGC 1 cut(s) 250
BstKTI GATC 7 cut(s) 15, 293, 302, 439, 920, 1007, 1102
BstMAI GTCTC 3 cut(s) 265, 1139, 1240
BstMBI GATC 7 cut(s) 12, 290, 299, 436, 917, 1004, 1099
BstMWI GCNNNNNNNGC 5 cut(s) 98, 247, 638, 1040, 1170
BstNSI RCATGY 2 cut(s) 789, 929
BstSCI CCNGG 2 cut(s) 410, 507
BstSNI TACGTA 1 cut(s) 46
BstV1I GCAGC 3 cut(s) 234, 616, 653
BstX2I RGATCY 3 cut(s) 12, 290, 917
BstYI RGATCY 3 cut(s) 12, 290, 917
Bsu15I ATCGAT 1 cut(s) 901
BsuI GTATCC 3 cut(s) 732, 853, 1139
BsuTUI ATCGAT 1 cut(s) 901
BtgI CCRYGG 1 cut(s) 846
BtgZI GCGATG 1 cut(s) 584
BtsCI GGATG 2 cut(s) 156, 1188
BtsI GCAGTG 1 cut(s) 1258
BtsIMutI CAGTG 5 cut(s) 292, 1044, 1173, 1246, 1258
BveI ACCTGC 1 cut(s) 1307
Cac8I GCNNGC 3 cut(s) 140, 467, 981
CaiI CAGNNNCTG 1 cut(s) 731
CfoI GCGC 1 cut(s) 250
Cfr10I RCCGGY 4 cut(s) 362, 616, 1030, 1248
Cfr13I GGNCC 4 cut(s) 191, 305, 338, 398
ClaI ATCGAT 1 cut(s) 901
CseI GACGC 2 cut(s) 447, 1121
Csp6I GTAC 2 cut(s) 231, 530
CspAI ACCGGT 2 cut(s) 362, 616
CviAII CATG 2 cut(s) 786, 926
CviQI GTAC 2 cut(s) 231, 530
DdeI CTNAG 5 cut(s) 334, 348, 732, 737, 1111
DinI GGCGCC 1 cut(s) 249
DpnI GATC 7 cut(s) 14, 292, 301, 438, 919, 1006, 1101
DpnII GATC 7 cut(s) 12, 290, 299, 436, 917, 1004, 1099
DraI TTTAAA 1 cut(s) 526
DrdI GACNNNNNNGTC 1 cut(s) 164
DseDI GACNNNNNNGTC 1 cut(s) 164
Eam1104I CTCTTC 1 cut(s) 1271
EarI CTCTTC 1 cut(s) 1271
Ecl136II GAGCTC 1 cut(s) 142
Eco105I TACGTA 1 cut(s) 46
Eco24I GRGCYC 1 cut(s) 144
Eco47I GGWCC 4 cut(s) 191, 305, 338, 398
Eco53kI GAGCTC 1 cut(s) 142
Eco57I CTGAAG 2 cut(s) 227, 338
Eco72I CACGTG 1 cut(s) 257
Eco88I CYCGRG 1 cut(s) 96
EcoICRI GAGCTC 1 cut(s) 142
EcoT38I GRGCYC 1 cut(s) 144
EgeI GGCGCC 1 cut(s) 249
EheI GGCGCC 1 cut(s) 249
Esp3I CGTCTC 1 cut(s) 1139
FaeI CATG 2 cut(s) 789, 929
FaiI YATR 9 cut(s) 787, 840, 858, 927, 939, 1002, 1130, 1151, 1295
FalI AAGNNNNNCTT 2 cut(s) 784, 816
FaqI GGGAC 1 cut(s) 291
FatI CATG 2 cut(s) 785, 925
FblI GTMKAC 1 cut(s) 504
Fnu4HI GCNGC 6 cut(s) 93, 223, 251, 567, 630, 642
FokI GGATG 2 cut(s) 163, 1195
FriOI GRGCYC 1 cut(s) 144
Fsp4HI GCNGC 6 cut(s) 93, 223, 251, 567, 630, 642
FspBI CTAG 4 cut(s) 417, 455, 921, 986
GlaI GCGC 1 cut(s) 249
GluI GCNGC 6 cut(s) 93, 223, 251, 567, 630, 642
GsaI CCCAGC 1 cut(s) 1080
GsuI CTGGAG 1 cut(s) 1222
HaeII RGCGCY 1 cut(s) 251
HgaI GACGC 2 cut(s) 447, 1121
HhaI GCGC 1 cut(s) 250
Hin1I GRCGYC 1 cut(s) 248
Hin1II CATG 2 cut(s) 789, 929
Hin6I GCGC 1 cut(s) 248
HinP1I GCGC 1 cut(s) 248
HindIII AAGCTT 1 cut(s) 56
HinfI GANTC 4 cut(s) 39, 173, 493, 806
HphI GGTGA 4 cut(s) 431, 499, 619, 634
Hpy166II GTNNAC 2 cut(s) 341, 505
Hpy188I TCNGA 7 cut(s) 12, 146, 946, 961, 1099, 1209, 1337
Hpy188III TCNNGA 6 cut(s) 51, 127, 417, 725, 921, 972
Hpy8I GTNNAC 2 cut(s) 341, 505
Hpy99I CGWCG 6 cut(s) 170, 173, 212, 463, 487, 1307
HpyAV CCTTC 5 cut(s) 141, 414, 1136, 1151, 1185
HpyCH4III ACNGT 5 cut(s) 287, 601, 1039, 1124, 1168
HpyCH4IV ACGT 5 cut(s) 45, 233, 256, 501, 1203
HpyCH4V TGCA 2 cut(s) 656, 929
HpyF10VI GCNNNNNNNGC 5 cut(s) 98, 247, 638, 1040, 1170
HpyF3I CTNAG 5 cut(s) 334, 348, 732, 737, 1111
HpySE526I ACGT 5 cut(s) 45, 233, 256, 501, 1203
Hsp92I GRCGYC 1 cut(s) 248
Hsp92II CATG 2 cut(s) 789, 929
HspAI GCGC 1 cut(s) 248
KasI GGCGCC 1 cut(s) 247
Kzo9I GATC 7 cut(s) 12, 290, 299, 436, 917, 1004, 1099
LmnI GCTCC 1 cut(s) 587
Lsp1109I GCAGC 3 cut(s) 234, 616, 653
LweI GCATC 3 cut(s) 718, 821, 893
MaeI CTAG 4 cut(s) 417, 455, 921, 986
MaeII ACGT 5 cut(s) 45, 233, 256, 501, 1203
MaeIII GTNAC 4 cut(s) 487, 769, 781, 1048
MalI GATC 7 cut(s) 14, 292, 301, 438, 919, 1006, 1101
MboI GATC 7 cut(s) 12, 290, 299, 436, 917, 1004, 1099
MboII GAAGA 7 cut(s) 261, 264, 384, 622, 702, 801, 1258
MfeI CAATTG 2 cut(s) 431, 443
MflI RGATCY 3 cut(s) 12, 290, 917
MhlI GDGCHC 2 cut(s) 144, 985
MluCI AATT 7 cut(s) 329, 369, 402, 431, 443, 676, 758
Mly113I GGCGCC 1 cut(s) 248
MlyI GAGTC 2 cut(s) 33, 502
MmeI TCCRAC 1 cut(s) 924
MnlI CCTC 7 cut(s) 110, 221, 385, 734, 1118, 1230, 1274
MseI TTAA 8 cut(s) 203, 324, 368, 498, 525, 765, 1085, 1289
MslI CAYNNNNRTG 1 cut(s) 829
MspA1I CMGCKG 2 cut(s) 225, 1115
MspR9I CCNGG 2 cut(s) 412, 509
MunI CAATTG 2 cut(s) 431, 443
Mva1269I GAATGC 1 cut(s) 795
MwoI GCNNNNNNNGC 5 cut(s) 98, 247, 638, 1040, 1170
NarI GGCGCC 1 cut(s) 248
NciI CCSGG 2 cut(s) 412, 509
NdeII GATC 7 cut(s) 12, 290, 299, 436, 917, 1004, 1099
NlaIII CATG 2 cut(s) 789, 929
NlaIV GGNNCC 7 cut(s) 14, 249, 292, 307, 399, 745, 1247
NmuCI GTSAC 2 cut(s) 487, 781
NspI RCATGY 2 cut(s) 789, 929
PaeR7I CTCGAG 1 cut(s) 96
PciI ACATGT 1 cut(s) 785
PcsI WCGNNNNNNNCGW 2 cut(s) 162, 488
PctI GAATGC 1 cut(s) 795
PfeI GAWTC 2 cut(s) 173, 806
PinAI ACCGGT 2 cut(s) 362, 616
PkrI GCNGC 6 cut(s) 94, 224, 252, 568, 631, 643
PleI GAGTC 2 cut(s) 33, 501
PluTI GGCGCC 1 cut(s) 251
PmaCI CACGTG 1 cut(s) 257
PmlI CACGTG 1 cut(s) 257
PpsI GAGTC 2 cut(s) 33, 501
Ppu21I YACGTR 2 cut(s) 46, 257
PscI ACATGT 1 cut(s) 785
Psp124BI GAGCTC 1 cut(s) 144
Psp1406I AACGTT 1 cut(s) 1203
PspCI CACGTG 1 cut(s) 257
PspFI CCCAGC 1 cut(s) 1076
PspN4I GGNNCC 7 cut(s) 14, 249, 292, 307, 399, 745, 1247
PspPI GGNCC 4 cut(s) 191, 305, 338, 398
PspXI VCTCGAGB 1 cut(s) 96
PsrI GAACNNNNNNTAC 2 cut(s) 495, 527
PstNI CAGNNNCTG 1 cut(s) 731
PsuI RGATCY 3 cut(s) 12, 290, 917
PvuII CAGCTG 1 cut(s) 1115
RsaI GTAC 2 cut(s) 232, 531
RsaNI GTAC 2 cut(s) 231, 530
RseI CAYNNNNRTG 1 cut(s) 829
SacI GAGCTC 1 cut(s) 144
SaqAI TTAA 8 cut(s) 203, 324, 368, 498, 525, 765, 1085, 1289
SatI GCNGC 6 cut(s) 93, 223, 251, 567, 630, 642
Sau3AI GATC 7 cut(s) 12, 290, 299, 436, 917, 1004, 1099
Sau96I GGNCC 4 cut(s) 191, 305, 338, 398
SchI GAGTC 2 cut(s) 33, 502
ScrFI CCNGG 2 cut(s) 412, 509
SduI GDGCHC 2 cut(s) 144, 985
SfaNI GCATC 3 cut(s) 718, 821, 893
SfoI GGCGCC 1 cut(s) 249
Sfr274I CTCGAG 1 cut(s) 96
SinI GGWCC 4 cut(s) 191, 305, 338, 398
SlaI CTCGAG 1 cut(s) 96
SmiMI CAYNNNNRTG 1 cut(s) 829
SmlI CTYRAG 2 cut(s) 59, 96
SmoI CTYRAG 2 cut(s) 59, 96
SnaBI TACGTA 1 cut(s) 46
SpeI ACTAGT 1 cut(s) 985
Sse9I AATT 7 cut(s) 329, 369, 402, 431, 443, 676, 758
SspDI GGCGCC 1 cut(s) 247
SspMI CTAG 4 cut(s) 417, 455, 921, 986
SstI GAGCTC 1 cut(s) 144
StyD4I CCNGG 2 cut(s) 410, 507
TaaI ACNGT 5 cut(s) 287, 601, 1039, 1124, 1168
TaiI ACGT 5 cut(s) 48, 236, 259, 504, 1206
TaqI TCGA 6 cut(s) 97, 156, 171, 298, 461, 901
TasI AATT 7 cut(s) 329, 369, 402, 431, 443, 676, 758
TauI GCSGC 3 cut(s) 95, 253, 569
TfiI GAWTC 2 cut(s) 173, 806
Tru1I TTAA 8 cut(s) 203, 324, 368, 498, 525, 765, 1085, 1289
Tru9I TTAA 8 cut(s) 203, 324, 368, 498, 525, 765, 1085, 1289
TscAI CASTG 5 cut(s) 292, 1044, 1173, 1246, 1265
TseFI GTSAC 2 cut(s) 487, 781
TseI GCWGC 3 cut(s) 222, 629, 641
Tsp45I GTSAC 2 cut(s) 487, 781
TspDTI ATGAA 1 cut(s) 873
TspGWI ACGGA 3 cut(s) 154, 283, 863
TspRI CASTG 5 cut(s) 292, 1044, 1173, 1246, 1265
VpaK11BI GGWCC 4 cut(s) 191, 305, 338, 398
XapI RAATTY 1 cut(s) 329
XbaI TCTAGA 2 cut(s) 416, 920
XceI RCATGY 2 cut(s) 789, 929
XcmI CCANNNNNNNNNTGG 1 cut(s) 32
XhoI CTCGAG 1 cut(s) 96
XmiI GTMKAC 1 cut(s) 504
XspI CTAG 4 cut(s) 417, 455, 921, 986
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.