Rh5DG014900

membrane protein At1g16860-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0009605
Physical Location & Seq
Forward (+)
1131642 .. 1135268
3627 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG014900.1

Sequence Viewer

Length: 927 bp
ATGGGTTCTCGAATCCCATCTCACCAGCTCAGCAACGGGCTGGTGGTGTCGGGTCCGCCCGAGCAGTTCAAGGAGCGGCAGCCGACAATGGGCTCACGCGCCGTGCCTTACACCGGCGGTGACGTCAAGAAATCCGGGGAGCTCGGGAAAATGTTCGACATCCAAATCGGGGATCACCCACCTCCGTTGAAGCCCTCCCGTCATTCCTCTTCCTCGCAGCACAACAGCGGATTGGTCCGATCAGGACCCAATTCGGGTCAGATATCCAAGAAATCGTCCGGGCCCATGACGCCGCAGCTTCAGCCTACTGGGCTCATCACCTCCGGCCAATTGGGCTCGGGCCCGCTCCCCAGCGGGCCCAGGAGGTCGTCGGGACAGCTGGGGCAGCTGGAGCAGACGGCGTCGATGAACAACAACAAGAAGGTGGTGTACGGGTCGGCGGTGACGGCGCTGAGCGATGACGTGAAGGTGAATTTTCGGGTTTCGAAGGCGGTGGTGTGGGTGTTCATGGTGGTGGTGGCGATGGGTTTGCTGGTGGGAGGGTTTCTGATGGTGGCGGTGAAGAAGTGGGTGATATTGGTGGCGGTGGCGCTGGTTTTGGTGCCGGTGGGGGTGGTGATTGTCTGGAATTACGTGTGGGGGAAGCGAGGGTTGCTGGGTTTCGTGAGGAGTTACCCTGATGCTGAGCTCAGGGGAGCCATAGATGGACAGTTCGTCAAGGTTACTGGGGTTGTTACCTGTGGCAGCATTCCTTTGGAATCATCCTACCAAAGGGTACCTAGGTGTGTATATGTTTCCACAGAATTATGTGAAACTCAAGGATGTGGTAGCAAGTCCACAAATAAAAAATCTAATCATTCAGAGGTTAGTTGTCAGACTCTTTGTGCTTATATTGAGTTTCAAAGTTTGCTTAATGATGACTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

308

Amino Acids

32.86

Weight (kDa)

9.53

Isoelectric Point (pI)

36.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 126
Acc65I GGTACC 1 cut(s) 775
AccB1I GGYRCC 2 cut(s) 601, 775
AccBSI CCGCTC 2 cut(s) 76, 346
AccII CGCG 1 cut(s) 99
AclWI GGATC 1 cut(s) 180
AcoI YGGCCR 1 cut(s) 325
AcsI RAATTY 1 cut(s) 472
AcuI CTGAAG 1 cut(s) 284
AcyI GRCGYC 3 cut(s) 123, 290, 401
AfaI GTAC 2 cut(s) 431, 777
AfiI CCNNNNNNNGG 5 cut(s) 89, 113, 169, 254, 771
AflIII ACRYGT 1 cut(s) 633
AgsI TTSAA 3 cut(s) 70, 190, 902
AhdI GACNNNNNGTC 1 cut(s) 713
AjiI CACGTC 1 cut(s) 463
AjnI CCWGG 1 cut(s) 359
AluBI AGCT 6 cut(s) 28, 142, 298, 379, 388, 688
AluI AGCT 6 cut(s) 28, 142, 298, 379, 388, 688
Alw21I GWGCWC 2 cut(s) 144, 690
AlwI GGATC 1 cut(s) 180
Ama87I CYCGRG 3 cut(s) 59, 143, 337
AoxI GGCC 4 cut(s) 281, 325, 340, 356
ApaI GGGCCC 3 cut(s) 285, 344, 360
ApeKI GCWGC 5 cut(s) 79, 217, 295, 385, 744
ApoI RAATTY 1 cut(s) 472
Asp700I GAANNNNTTC 1 cut(s) 152
Asp718I GGTACC 1 cut(s) 775
AspA2I CCTAGG 1 cut(s) 779
AspLEI GCGC 3 cut(s) 101, 451, 592
AspS9I GGNCC 9 cut(s) 53, 235, 245, 281, 282, 340, 341, 356, 357
AsuC2I CCSGG 2 cut(s) 136, 280
AsuHPI GGTGA 9 cut(s) 14, 131, 167, 310, 454, 481, 571, 583, 628
AsuII TTCGAA 1 cut(s) 485
AvaI CYCGRG 3 cut(s) 59, 143, 337
AvaII GGWCC 3 cut(s) 53, 235, 245
AvrII CCTAGG 1 cut(s) 779
BaeGI GKGCMC 3 cut(s) 285, 344, 360
BaeI ACNNNNGTAYC 2 cut(s) 767, 800
BanI GGYRCC 2 cut(s) 601, 775
BanII GRGCYC 8 cut(s) 95, 144, 285, 315, 338, 344, 360, 690
BarI GAAGNNNNNNTAC 2 cut(s) 413, 445
Bbv12I GWGCWC 2 cut(s) 144, 690
BbvI GCAGC 5 cut(s) 91, 229, 307, 397, 756
BccI CCATC 4 cut(s) 25, 517, 544, 698
BceAI ACGGC 3 cut(s) 86, 414, 462
BcgI CGANNNNNNTGC 2 cut(s) 511, 545
BciT130I CCWGG 1 cut(s) 361
BcnI CCSGG 2 cut(s) 136, 280
BfaI CTAG 1 cut(s) 780
BfoI RGCGCY 2 cut(s) 452, 593
BglI GCCNNNNNGGC 2 cut(s) 310, 333
BisI GCNGC 7 cut(s) 77, 80, 218, 293, 296, 386, 745
BlnI CCTAGG 1 cut(s) 779
BlpI GCTNAGC 3 cut(s) 29, 452, 684
BlsI GCNGC 7 cut(s) 78, 81, 219, 294, 297, 387, 746
Bme1390I CCNGG 3 cut(s) 136, 280, 361
Bme18I GGWCC 3 cut(s) 53, 235, 245
BmeRI GACNNNNNGTC 1 cut(s) 713
BmeT110I CYCGRG 3 cut(s) 59, 143, 337
BmgBI CACGTC 1 cut(s) 463
BmgT120I GGNCC 9 cut(s) 53, 235, 245, 281, 282, 340, 341, 356, 357
BmiI GGNNCC 8 cut(s) 54, 247, 283, 342, 358, 603, 697, 777
BmrFI CCNGG 3 cut(s) 136, 280, 361
BmrI ACTGGG 2 cut(s) 318, 735
BmsI GCATC 1 cut(s) 670
BmuI ACTGGG 2 cut(s) 318, 735
BpmI CTGGAG 1 cut(s) 410
Bpu10I CCTNAGC 1 cut(s) 689
Bpu1102I GCTNAGC 3 cut(s) 29, 452, 684
Bpu14I TTCGAA 1 cut(s) 485
BpuEI CTTGAG 1 cut(s) 801
BpuMI CCSGG 2 cut(s) 136, 280
BsaAI YACGTR 1 cut(s) 634
BsaHI GRCGYC 3 cut(s) 123, 290, 401
BsaJI CCNNGG 3 cut(s) 135, 359, 779
Bsc4I CCNNNNNNNGG 5 cut(s) 89, 113, 169, 254, 771
Bse118I RCCGGY 2 cut(s) 113, 604
Bse1I ACTGG 2 cut(s) 313, 730
BseBI CCWGG 1 cut(s) 361
BseDI CCNNGG 3 cut(s) 135, 359, 779
BseGI GGATG 3 cut(s) 159, 761, 827
BseLI CCNNNNNNNGG 5 cut(s) 89, 113, 169, 254, 771
BseMII CTCAG 4 cut(s) 43, 443, 675, 703
BseNI ACTGG 2 cut(s) 313, 730
BseRI GAGGAG 1 cut(s) 682
BseSI GKGCMC 3 cut(s) 285, 344, 360
BseXI GCAGC 5 cut(s) 91, 229, 307, 397, 756
BseYI CCCAGC 3 cut(s) 350, 379, 655
Bsh1236I CGCG 1 cut(s) 99
BshFI GGCC 4 cut(s) 283, 327, 342, 358
BshNI GGYRCC 2 cut(s) 601, 775
BsiHKAI GWGCWC 2 cut(s) 144, 690
BsiHKCI CYCGRG 3 cut(s) 59, 143, 337
BsiSI CCGG 5 cut(s) 114, 135, 279, 324, 605
BslFI GGGAC 1 cut(s) 387
BslI CCNNNNNNNGG 5 cut(s) 89, 113, 169, 254, 771
BsmFI GGGAC 1 cut(s) 387
BsmI GAATGC 1 cut(s) 747
BsnI GGCC 4 cut(s) 283, 327, 342, 358
BsoBI CYCGRG 3 cut(s) 59, 143, 337
Bsp119I TTCGAA 1 cut(s) 485
Bsp120I GGGCCC 3 cut(s) 281, 340, 356
Bsp1286I GDGCHC 8 cut(s) 95, 144, 285, 315, 338, 344, 360, 690
Bsp143I GATC 2 cut(s) 172, 239
Bsp1720I GCTNAGC 3 cut(s) 29, 452, 684
BspANI GGCC 4 cut(s) 283, 327, 342, 358
BspCNI CTCAG 4 cut(s) 42, 444, 676, 702
BspFNI CGCG 1 cut(s) 99
BspLI GGNNCC 8 cut(s) 54, 247, 283, 342, 358, 603, 697, 777
BspPI GGATC 1 cut(s) 180
BspT104I TTCGAA 1 cut(s) 485
BspT107I GGYRCC 2 cut(s) 601, 775
BsrBI CCGCTC 2 cut(s) 76, 346
BsrFI RCCGGY 2 cut(s) 113, 604
BsrI ACTGG 2 cut(s) 313, 730
BssAI RCCGGY 2 cut(s) 113, 604
BssECI CCNNGG 3 cut(s) 135, 359, 779
BssMI GATC 2 cut(s) 172, 239
BssNI GRCGYC 3 cut(s) 123, 290, 401
BssT1I CCWWGG 1 cut(s) 779
Bst2UI CCWGG 1 cut(s) 361
Bst4CI ACNGT 1 cut(s) 711
Bst6I CTCTTC 1 cut(s) 214
BstACI GRCGYC 3 cut(s) 123, 290, 401
BstBAI YACGTR 1 cut(s) 634
BstBI TTCGAA 1 cut(s) 485
BstC8I GCNNGC 2 cut(s) 344, 356
BstDEI CTNAG 4 cut(s) 29, 452, 684, 689
BstENI CCTNNNNNAGG 1 cut(s) 769
BstF5I GGATG 3 cut(s) 159, 761, 827
BstFNI CGCG 1 cut(s) 99
BstH2I RGCGCY 2 cut(s) 452, 593
BstHHI GCGC 3 cut(s) 101, 451, 592
BstKTI GATC 2 cut(s) 175, 242
BstMBI GATC 2 cut(s) 172, 239
BstMWI GCNNNNNNNGC 8 cut(s) 289, 301, 310, 333, 385, 391, 446, 652
BstNI CCWGG 1 cut(s) 361
BstSCI CCNGG 3 cut(s) 134, 278, 359
BstSLI GKGCMC 3 cut(s) 285, 344, 360
BstUI CGCG 1 cut(s) 99
BstV1I GCAGC 5 cut(s) 91, 229, 307, 397, 756
BsuRI GGCC 4 cut(s) 283, 327, 342, 358
BtgZI GCGATG 2 cut(s) 471, 536
BtrI CACGTC 1 cut(s) 463
BtsCI GGATG 3 cut(s) 159, 761, 827
Cac8I GCNNGC 2 cut(s) 344, 356
CfoI GCGC 3 cut(s) 101, 451, 592
Cfr10I RCCGGY 2 cut(s) 113, 604
Cfr13I GGNCC 9 cut(s) 53, 235, 245, 281, 282, 340, 341, 356, 357
CseI GACGC 2 cut(s) 298, 390
Csp6I GTAC 2 cut(s) 430, 776
CspCI CAANNNNNGTGG 2 cut(s) 168, 203
CviAII CATG 2 cut(s) 286, 508
CviQI GTAC 2 cut(s) 430, 776
DdeI CTNAG 4 cut(s) 29, 452, 684, 689
DpnI GATC 2 cut(s) 174, 241
DpnII GATC 2 cut(s) 172, 239
DriI GACNNNNNGTC 1 cut(s) 713
EaeI YGGCCR 1 cut(s) 325
Eam1104I CTCTTC 1 cut(s) 214
Eam1105I GACNNNNNGTC 1 cut(s) 713
EarI CTCTTC 1 cut(s) 214
EciI GGCGGA 1 cut(s) 45
Ecl136II GAGCTC 2 cut(s) 142, 688
Eco130I CCWWGG 1 cut(s) 779
Eco24I GRGCYC 8 cut(s) 95, 144, 285, 315, 338, 344, 360, 690
Eco32I GATATC 1 cut(s) 264
Eco47I GGWCC 3 cut(s) 53, 235, 245
Eco53kI GAGCTC 2 cut(s) 142, 688
Eco57I CTGAAG 1 cut(s) 284
Eco88I CYCGRG 3 cut(s) 59, 143, 337
EcoICRI GAGCTC 2 cut(s) 142, 688
EcoNI CCTNNNNNAGG 1 cut(s) 769
EcoO109I RGGNCCY 1 cut(s) 245
EcoRII CCWGG 1 cut(s) 359
EcoRV GATATC 1 cut(s) 264
EcoT14I CCWWGG 1 cut(s) 779
EcoT38I GRGCYC 8 cut(s) 95, 144, 285, 315, 338, 344, 360, 690
ErhI CCWWGG 1 cut(s) 779
FaeI CATG 2 cut(s) 289, 511
FaiI YATR 7 cut(s) 287, 509, 701, 790, 792, 808, 891
FaqI GGGAC 1 cut(s) 387
FatI CATG 2 cut(s) 285, 507
FauI CCCGC 2 cut(s) 347, 351
Fnu4HI GCNGC 7 cut(s) 77, 80, 218, 293, 296, 386, 745
FokI GGATG 3 cut(s) 146, 748, 834
FriOI GRGCYC 8 cut(s) 95, 144, 285, 315, 338, 344, 360, 690
Fsp4HI GCNGC 7 cut(s) 77, 80, 218, 293, 296, 386, 745
FspBI CTAG 1 cut(s) 780
GlaI GCGC 3 cut(s) 100, 450, 591
GluI GCNGC 7 cut(s) 77, 80, 218, 293, 296, 386, 745
GsaI CCCAGC 3 cut(s) 354, 383, 659
GsuI CTGGAG 1 cut(s) 410
HaeII RGCGCY 2 cut(s) 452, 593
HaeIII GGCC 4 cut(s) 283, 327, 342, 358
HapII CCGG 5 cut(s) 114, 135, 279, 324, 605
HgaI GACGC 2 cut(s) 298, 390
HhaI GCGC 3 cut(s) 101, 451, 592
Hin1I GRCGYC 3 cut(s) 123, 290, 401
Hin1II CATG 2 cut(s) 289, 511
Hin6I GCGC 3 cut(s) 99, 449, 590
HinP1I GCGC 3 cut(s) 99, 449, 590
HinfI GANTC 3 cut(s) 12, 758, 877
HpaII CCGG 5 cut(s) 114, 135, 279, 324, 605
HphI GGTGA 9 cut(s) 14, 131, 167, 310, 454, 481, 571, 583, 628
Hpy166II GTNNAC 2 cut(s) 430, 837
Hpy188I TCNGA 5 cut(s) 239, 261, 549, 862, 876
Hpy188III TCNNGA 7 cut(s) 9, 127, 145, 243, 372, 625, 664
Hpy8I GTNNAC 2 cut(s) 430, 837
Hpy99I CGWCG 2 cut(s) 373, 406
HpyAV CCTTC 3 cut(s) 415, 460, 481
HpyCH4III ACNGT 1 cut(s) 711
HpyCH4IV ACGT 3 cut(s) 123, 462, 633
HpyF10VI GCNNNNNNNGC 8 cut(s) 289, 301, 310, 333, 385, 391, 446, 652
HpyF3I CTNAG 4 cut(s) 29, 452, 684, 689
HpySE526I ACGT 3 cut(s) 123, 462, 633
Hsp92I GRCGYC 3 cut(s) 123, 290, 401
Hsp92II CATG 2 cut(s) 289, 511
HspAI GCGC 3 cut(s) 99, 449, 590
KpnI GGTACC 1 cut(s) 779
Kzo9I GATC 2 cut(s) 172, 239
LmnI GCTCC 5 cut(s) 73, 139, 351, 391, 695
Lsp1109I GCAGC 5 cut(s) 91, 229, 307, 397, 756
LweI GCATC 1 cut(s) 670
MaeI CTAG 1 cut(s) 780
MaeII ACGT 3 cut(s) 123, 462, 633
MaeIII GTNAC 5 cut(s) 119, 442, 671, 721, 733
MalI GATC 2 cut(s) 174, 241
MbiI CCGCTC 2 cut(s) 76, 346
MboI GATC 2 cut(s) 172, 239
MboII GAAGA 2 cut(s) 201, 574
MfeI CAATTG 1 cut(s) 329
MhlI GDGCHC 8 cut(s) 95, 144, 285, 315, 338, 344, 360, 690
MluCI AATT 5 cut(s) 250, 329, 472, 628, 803
MlyI GAGTC 1 cut(s) 871
MroXI GAANNNNTTC 1 cut(s) 152
MseI TTAA 1 cut(s) 912
MslI CAYNNNNRTG 1 cut(s) 512
MspA1I CMGCKG 4 cut(s) 228, 354, 379, 388
MspI CCGG 5 cut(s) 114, 135, 279, 324, 605
MspR9I CCNGG 3 cut(s) 136, 280, 361
MunI CAATTG 1 cut(s) 329
Mva1269I GAATGC 1 cut(s) 747
MvaI CCWGG 1 cut(s) 361
MvnI CGCG 1 cut(s) 99
MwoI GCNNNNNNNGC 8 cut(s) 289, 301, 310, 333, 385, 391, 446, 652
NciI CCSGG 2 cut(s) 136, 280
NdeII GATC 2 cut(s) 172, 239
NlaIII CATG 2 cut(s) 289, 511
NlaIV GGNNCC 8 cut(s) 54, 247, 283, 342, 358, 603, 697, 777
NmuCI GTSAC 2 cut(s) 119, 442
NspV TTCGAA 1 cut(s) 485
PctI GAATGC 1 cut(s) 747
PdmI GAANNNNTTC 1 cut(s) 152
PfeI GAWTC 2 cut(s) 12, 758
PflFI GACNNNGTC 1 cut(s) 400
PkrI GCNGC 7 cut(s) 78, 81, 219, 294, 297, 387, 746
PleI GAGTC 1 cut(s) 871
PpsI GAGTC 1 cut(s) 871
Ppu21I YACGTR 1 cut(s) 634
PpuMI RGGWCCY 1 cut(s) 245
Psp124BI GAGCTC 2 cut(s) 144, 690
Psp5II RGGWCCY 1 cut(s) 245
Psp6I CCWGG 1 cut(s) 359
PspFI CCCAGC 3 cut(s) 350, 379, 655
PspGI CCWGG 1 cut(s) 359
PspN4I GGNNCC 8 cut(s) 54, 247, 283, 342, 358, 603, 697, 777
PspOMI GGGCCC 3 cut(s) 281, 340, 356
PspPI GGNCC 9 cut(s) 53, 235, 245, 281, 282, 340, 341, 356, 357
PspPPI RGGWCCY 1 cut(s) 245
PsyI GACNNNGTC 1 cut(s) 400
PvuII CAGCTG 2 cut(s) 379, 388
RsaI GTAC 2 cut(s) 431, 777
RsaNI GTAC 2 cut(s) 430, 776
RseI CAYNNNNRTG 1 cut(s) 512
SacI GAGCTC 2 cut(s) 144, 690
SaqAI TTAA 1 cut(s) 912
SatI GCNGC 7 cut(s) 77, 80, 218, 293, 296, 386, 745
Sau3AI GATC 2 cut(s) 172, 239
Sau96I GGNCC 9 cut(s) 53, 235, 245, 281, 282, 340, 341, 356, 357
SchI GAGTC 1 cut(s) 871
ScrFI CCNGG 3 cut(s) 136, 280, 361
SduI GDGCHC 8 cut(s) 95, 144, 285, 315, 338, 344, 360, 690
SfaNI GCATC 1 cut(s) 670
SfuI TTCGAA 1 cut(s) 485
SgrAI CRCCGGYG 1 cut(s) 113
SinI GGWCC 3 cut(s) 53, 235, 245
SmiMI CAYNNNNRTG 1 cut(s) 512
SmlI CTYRAG 1 cut(s) 816
SmoI CTYRAG 1 cut(s) 816
Sse9I AATT 5 cut(s) 250, 329, 472, 628, 803
SspMI CTAG 1 cut(s) 780
SstI GAGCTC 2 cut(s) 144, 690
StyD4I CCNGG 3 cut(s) 134, 278, 359
StyI CCWWGG 1 cut(s) 779
TaaI ACNGT 1 cut(s) 711
TaiI ACGT 3 cut(s) 126, 465, 636
TaqI TCGA 4 cut(s) 10, 156, 404, 485
TasI AATT 5 cut(s) 250, 329, 472, 628, 803
TauI GCSGC 2 cut(s) 79, 295
TfiI GAWTC 2 cut(s) 12, 758
Tru1I TTAA 1 cut(s) 912
Tru9I TTAA 1 cut(s) 912
TseFI GTSAC 2 cut(s) 119, 442
TseI GCWGC 5 cut(s) 79, 217, 295, 385, 744
Tsp45I GTSAC 2 cut(s) 119, 442
TspDTI ATGAA 2 cut(s) 422, 496
TspGWI ACGGA 1 cut(s) 174
Tth111I GACNNNGTC 1 cut(s) 400
VpaK11BI GGWCC 3 cut(s) 53, 235, 245
XagI CCTNNNNNAGG 1 cut(s) 769
XapI RAATTY 1 cut(s) 472
XmaJI CCTAGG 1 cut(s) 779
XmnI GAANNNNTTC 1 cut(s) 152
XspI CTAG 1 cut(s) 780
ZraI GACGTC 1 cut(s) 124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.