AT4G22760

PPR repeat family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0020196
Physical Location & Seq
Forward (+)
11960229 .. 11963162
2934 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G22760.1

Sequence Viewer

Length: 1737 bp
ATGTTGGATTCTAAGCTGAGATTCTTCCTTCAAAGATGTGTAGTGCTTGAACAGGCGAAGCAAGTTCATGCACAGTTAGTAGTGAACCGCTATAACCATCTTGAACCCATCTTGGTTCACCAAACTCTTCACTTCACTAAAGAGTTCTCTAGGAATATTGTAACCTATGTTAAAAGAATCCTCAAGGGGTTTAATGGTCATGATTCTTTCTCCTGGGGGTGTTTAGTTCGGTTCTTAAGCCAGCATAGGAAGTTTAAAGAAACGGTTGACGTGTATATTGATATGCATAACTCTGGGATTCCTCCAAGTTCACATGCTGTAACCTCAGTGTTAAGAGCCTGTGGTAAAATGGAGAATATGGTTGATGGTAAACCGATTCATGCTCAGGCTCTGAAAAATGGATTGTGTGGTTGTGTTTATGTTCAGACAGGTCTGGTGGGTTTGTATTCTAGACTTGGTTACATTGAGCTGGCAAAGAAGGCGTTTGATGATATTGCTGAGAAGAACACAGTTTCGTGGAACTCGCTATTACACGGGTATCTGGAAAGCGGGGAACTAGATGAGGCTCGCAGAGTCTTTGACAAGATCCCTGAGAAAGATGCTGTGTCATGGAATCTGATTATCTCGAGCTATGCAAAAAAAGGTGACATGGGCAATGCATGCTCTTTGTTTTCAGCAATGCCTTTGAAAAGTCCGGCTTCTTGGAATATATTGATAGGCGGGTATGTGAATTGCAGGGAAATGAAACTAGCAAGAACCTACTTCGATGCAATGCCCCAGAAGAATGGTGTCTCTTGGATTACAATGATTTCAGGGTACACAAAATTGGGAGATGTTCAGTCTGCTGAGGAGCTCTTTAGACTGATGTCTAAGAAAGACAAACTCGTCTATGATGCCATGATAGCTTGTTATACTCAAAACGGCAAGCCAAAGGATGCTCTGAAGCTATTCGCTCAGATGCTTGAAAGGAATTCATATATTCAGCCGGATGAGATCACACTCTCAAGTGTTGTATCAGCTAATTCACAGTTGGGTAATACCAGTTTTGGTACATGGGTTGAGTCATATATAACCGAACATGGAATCAAGATCGATGATCTCTTAAGTACCTCTCTAATTGATCTTTACATGAAAGGCGGAGACTTTGCCAAGGCATTCAAAATGTTCAGCAATCTAAACAAAAAGGACACAGTTTCTTACTCTGCTATGATCATGGGCTGCGGGATAAACGGCATGGCCACAGAAGCAAACAGTTTGTTTACAGCTATGATTGAAAAGAAGATCCCTCCAAATGTAGTAACATTCACAGGACTATTATCAGCGTATAGCCATTCAGGTCTTGTTCAAGAAGGCTACAAATGCTTTAACTCCATGAAAGACCACAACTTGGAACCCTCCGCTGATCATTATGGGATAATGGTGGATATGTTGGGACGAGCCGGGAGGCTAGAAGAAGCATATGAGCTGATCAAGAGTATGCCGATGCAGCCAAATGCTGGAGTTTGGGGAGCTTTGCTTTTAGCCAGTGGGTTACACAACAACGTTGAATTTGGAGAGATTGCGTGCAGTCACTGCGTTAAACTCGAGACAGATCCTACAGGTTATCTTTCGCATCTTGCTATGATATATTCGTCTGTTGGTAGATGGGACGATGCCAGGACTGTTAGAGACAGTATAAAAGAGAAGAAACTGTGTAAGACACTTGGGTGTAGCTGGGTTGAGGGTTCATATCATTGA

Protein Analysis

578

Amino Acids

64.59

Weight (kDa)

8.23

Isoelectric Point (pI)

31.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 68 - 116 7.7e-06 PPR repeat family
PPR PF01535 264 - 292 2.2e-06 PPR repeat
PPR_2 PF13041 295 - 339 4.2e-07 PPR repeat family
PPR PF01535 296 - 324 7e-06 PPR repeat
PPR_2 PF13041 395 - 443 5.3e-10 PPR repeat family
PPR_long PF17177 399 - 492 2.9e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 430 - 477 3.2e-09 PPR repeat family
E_motif PF20431 512 - 574 1.9e-10 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020196)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G22760
malus_domestica MD04G1078100.v1.1
prunus_persica Prupe.5G080600_v2.0.a1
pyrus_communis pycom04g07010 pycom06g06320 pycom06g06340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 884
AccB7I CCANNNNNTGG 2 cut(s) 1387, 1496
AciI CCGC 6 cut(s) 88, 549, 720, 1137, 1221, 1398
AclI AACGTT 1 cut(s) 1542
AclWI GGATC 3 cut(s) 580, 1276, 1586
AcoI YGGCCR 1 cut(s) 1236
AcsI RAATTY 2 cut(s) 970, 1547
AcuI CTGAAG 1 cut(s) 962
AfaI GTAC 3 cut(s) 818, 1051, 1108
AfiI CCNNNNNNNGG 2 cut(s) 1387, 1496
AflII CTTAAG 2 cut(s) 235, 1102
AflIII ACRYGT 1 cut(s) 270
AgsI TTSAA 9 cut(s) 32, 50, 104, 688, 965, 1159, 1274, 1346, 1547
AjiI CACGTC 1 cut(s) 271
AjnI CCWGG 2 cut(s) 212, 1655
AleI CACNNNNGTG 1 cut(s) 1705
Alw21I GWGCWC 1 cut(s) 855
Alw26I GTCTC 4 cut(s) 796, 1134, 1580, 1662
AlwI GGATC 3 cut(s) 580, 1276, 1586
AlwNI CAGNNNCTG 2 cut(s) 391, 1572
Ama87I CYCGRG 2 cut(s) 625, 1583
AoxI GGCC 1 cut(s) 1236
ApeKI GCWGC 2 cut(s) 1218, 1486
ApoI RAATTY 2 cut(s) 970, 1547
Asp700I GAANNNNTTC 1 cut(s) 947
AsuC2I CCSGG 1 cut(s) 1441
AsuHPI GGTGA 2 cut(s) 110, 656
AvaI CYCGRG 2 cut(s) 625, 1583
BaeI ACNNNNGTAYC 4 cut(s) 521, 554, 1041, 1074
BalI TGGCCA 1 cut(s) 1238
BanII GRGCYC 1 cut(s) 855
Bbv12I GWGCWC 1 cut(s) 855
BbvCI CCTCAGC 1 cut(s) 846
BbvI GCAGC 2 cut(s) 1205, 1498
BccI CCATC 4 cut(s) 105, 116, 359, 1638
BceAI ACGGC 2 cut(s) 937, 1246
BciT130I CCWGG 2 cut(s) 214, 1657
BclI TGATCA 3 cut(s) 1209, 1402, 1467
BcnI CCSGG 1 cut(s) 1441
BcoDI GTCTC 4 cut(s) 796, 1134, 1580, 1662
BfaI CTAG 5 cut(s) 150, 450, 557, 749, 1448
BfmI CTRYAG 1 cut(s) 1596
BfrI CTTAAG 2 cut(s) 235, 1102
BisI GCNGC 2 cut(s) 1219, 1487
BlsI GCNGC 2 cut(s) 1220, 1488
Bme1390I CCNGG 3 cut(s) 214, 1441, 1657
BmeT110I CYCGRG 2 cut(s) 625, 1583
BmgBI CACGTC 1 cut(s) 271
BmiI GGNNCC 1 cut(s) 1392
BmrFI CCNGG 3 cut(s) 214, 1441, 1657
BmsI GCATC 8 cut(s) 589, 757, 883, 925, 948, 1473, 1621, 1642
BoxI GACNNNNGTC 1 cut(s) 865
BpmI CTGGAG 1 cut(s) 1518
Bpu10I CCTNAGC 2 cut(s) 384, 846
BpuEI CTTGAG 2 cut(s) 167, 988
BpuMI CCSGG 1 cut(s) 1441
Bsa29I ATCGAT 1 cut(s) 1092
BsaJI CCNNGG 2 cut(s) 213, 1149
Bsc4I CCNNNNNNNGG 2 cut(s) 1387, 1496
Bse1I ACTGG 2 cut(s) 1041, 1524
Bse3DI GCAATG 3 cut(s) 661, 684, 777
BseBI CCWGG 2 cut(s) 214, 1657
BseCI ATCGAT 1 cut(s) 1092
BseDI CCNNGG 2 cut(s) 213, 1149
BseGI GGATG 2 cut(s) 940, 994
BseLI CCNNNNNNNGG 2 cut(s) 1387, 1496
BseMI GCAATG 3 cut(s) 661, 684, 777
BseMII CTCAG 7 cut(s) 8, 339, 398, 489, 582, 837, 968
BseNI ACTGG 2 cut(s) 1041, 1524
BseRI GAGGAG 1 cut(s) 863
BseXI GCAGC 2 cut(s) 1205, 1498
BseYI CCCAGC 1 cut(s) 1713
BsgI GTGCAG 1 cut(s) 1585
BshFI GGCC 1 cut(s) 1238
BshVI ATCGAT 1 cut(s) 1092
BsiHKAI GWGCWC 1 cut(s) 855
BsiHKCI CYCGRG 2 cut(s) 625, 1583
BsiSI CCGG 3 cut(s) 695, 986, 1440
BslFI GGGAC 2 cut(s) 1446, 1661
BslI CCNNNNNNNGG 2 cut(s) 1387, 1496
BsmAI GTCTC 4 cut(s) 796, 1134, 1580, 1662
BsmFI GGGAC 2 cut(s) 1446, 1661
BsmI GAATGC 1 cut(s) 1154
BsnI GGCC 1 cut(s) 1238
BsoBI CYCGRG 2 cut(s) 625, 1583
Bsp1286I GDGCHC 1 cut(s) 855
BspACI CCGC 6 cut(s) 88, 549, 720, 1137, 1221, 1398
BspANI GGCC 1 cut(s) 1238
BspCNI CTCAG 7 cut(s) 9, 338, 397, 490, 583, 838, 967
BspDI ATCGAT 1 cut(s) 1092
BspHI TCATGA 1 cut(s) 199
BspLI GGNNCC 1 cut(s) 1392
BspPI GGATC 3 cut(s) 580, 1276, 1586
BspTI CTTAAG 2 cut(s) 235, 1102
BsrDI GCAATG 3 cut(s) 661, 684, 777
BsrI ACTGG 2 cut(s) 1041, 1524
BssECI CCNNGG 2 cut(s) 213, 1149
BssT1I CCWWGG 1 cut(s) 1149
Bst2UI CCWGG 2 cut(s) 214, 1657
Bst4CI ACNGT 9 cut(s) 75, 265, 511, 1029, 1192, 1253, 1663, 1673, 1692
Bst6I CTCTTC 1 cut(s) 132
BstAFI CTTAAG 2 cut(s) 235, 1102
BstAPI GCANNNNNTGC 2 cut(s) 660, 1572
BstC8I GCNNGC 6 cut(s) 242, 471, 568, 661, 926, 1564
BstDEI CTNAG 9 cut(s) 12, 17, 325, 384, 498, 591, 846, 870, 954
BstF5I GGATG 2 cut(s) 940, 994
BstMAI GTCTC 4 cut(s) 796, 1134, 1580, 1662
BstMWI GCNNNNNNNGC 7 cut(s) 479, 660, 902, 1244, 1359, 1486, 1572
BstNI CCWGG 2 cut(s) 214, 1657
BstNSI RCATGY 2 cut(s) 317, 663
BstPAI GACNNNNGTC 1 cut(s) 865
BstSCI CCNGG 3 cut(s) 212, 1439, 1655
BstSFI CTRYAG 1 cut(s) 1596
BstV1I GCAGC 2 cut(s) 1205, 1498
BstX2I RGATCY 3 cut(s) 585, 1281, 1591
BstXI CCANNNNNNTGG 1 cut(s) 785
BstYI RGATCY 3 cut(s) 585, 1281, 1591
Bsu15I ATCGAT 1 cut(s) 1092
BsuRI GGCC 1 cut(s) 1238
BsuTUI ATCGAT 1 cut(s) 1092
BtrI CACGTC 1 cut(s) 271
BtsCI GGATG 2 cut(s) 940, 994
BtsI GCAGTG 1 cut(s) 1570
BtsIMutI CAGTG 3 cut(s) 333, 1531, 1570
Cac8I GCNNGC 6 cut(s) 242, 471, 568, 661, 926, 1564
CaiI CAGNNNCTG 2 cut(s) 391, 1572
CciI TCATGA 1 cut(s) 199
ClaI ATCGAT 1 cut(s) 1092
Csp6I GTAC 3 cut(s) 817, 1050, 1107
CviQI GTAC 3 cut(s) 817, 1050, 1107
DdeI CTNAG 9 cut(s) 12, 17, 325, 384, 498, 591, 846, 870, 954
DraI TTTAAA 1 cut(s) 256
DrdI GACNNNNNNGTC 1 cut(s) 884
DseDI GACNNNNNNGTC 1 cut(s) 884
EaeI YGGCCR 1 cut(s) 1236
Eam1104I CTCTTC 1 cut(s) 132
EarI CTCTTC 1 cut(s) 132
EciI GGCGGA 1 cut(s) 1152
Ecl136II GAGCTC 1 cut(s) 853
Eco130I CCWWGG 1 cut(s) 1149
Eco24I GRGCYC 1 cut(s) 855
Eco53kI GAGCTC 1 cut(s) 853
Eco57I CTGAAG 1 cut(s) 962
Eco88I CYCGRG 2 cut(s) 625, 1583
EcoICRI GAGCTC 1 cut(s) 853
EcoRI GAATTC 1 cut(s) 970
EcoRII CCWGG 2 cut(s) 212, 1655
EcoT14I CCWWGG 1 cut(s) 1149
EcoT22I ATGCAT 2 cut(s) 288, 661
EcoT38I GRGCYC 1 cut(s) 855
ErhI CCWWGG 1 cut(s) 1149
FalI AAGNNNNNCTT 2 cut(s) 682, 714
FaqI GGGAC 2 cut(s) 1446, 1661
FauI CCCGC 3 cut(s) 542, 713, 1214
FauNDI CATATG 1 cut(s) 1459
FbaI TGATCA 3 cut(s) 1209, 1402, 1467
Fnu4HI GCNGC 2 cut(s) 1219, 1487
FokI GGATG 2 cut(s) 947, 1001
FriOI GRGCYC 1 cut(s) 855
Fsp4HI GCNGC 2 cut(s) 1219, 1487
FspBI CTAG 5 cut(s) 150, 450, 557, 749, 1448
GluI GCNGC 2 cut(s) 1219, 1487
GsaI CCCAGC 1 cut(s) 1717
GsuI CTGGAG 1 cut(s) 1518
HaeIII GGCC 1 cut(s) 1238
HapII CCGG 3 cut(s) 695, 986, 1440
HincII GTYRAC 1 cut(s) 268
HindII GTYRAC 1 cut(s) 268
HpaII CCGG 3 cut(s) 695, 986, 1440
HphI GGTGA 2 cut(s) 110, 656
Hpy166II GTNNAC 7 cut(s) 85, 118, 268, 311, 371, 819, 1260
Hpy188I TCNGA 5 cut(s) 393, 426, 618, 942, 957
Hpy188III TCNNGA 9 cut(s) 101, 200, 450, 542, 625, 1087, 1346, 1471, 1585
Hpy8I GTNNAC 7 cut(s) 85, 118, 268, 311, 371, 819, 1260
HpyAV CCTTC 3 cut(s) 38, 472, 1343
HpyCH4III ACNGT 9 cut(s) 75, 265, 511, 1029, 1192, 1253, 1663, 1673, 1692
HpyCH4IV ACGT 2 cut(s) 270, 1542
HpyCH4V TGCA 8 cut(s) 71, 286, 635, 659, 735, 770, 1486, 1566
HpyF10VI GCNNNNNNNGC 7 cut(s) 479, 660, 902, 1244, 1359, 1486, 1572
HpyF3I CTNAG 9 cut(s) 12, 17, 325, 384, 498, 591, 846, 870, 954
HpySE526I ACGT 2 cut(s) 270, 1542
Ksp22I TGATCA 3 cut(s) 1209, 1402, 1467
LmnI GCTCC 2 cut(s) 850, 1508
Lsp1109I GCAGC 2 cut(s) 1205, 1498
LweI GCATC 8 cut(s) 589, 757, 883, 925, 948, 1473, 1621, 1642
MaeI CTAG 5 cut(s) 150, 450, 557, 749, 1448
MaeII ACGT 2 cut(s) 270, 1542
MaeIII GTNAC 7 cut(s) 160, 319, 458, 644, 1297, 1530, 1568
MboII GAAGA 7 cut(s) 16, 119, 514, 793, 1291, 1463, 1696
MflI RGATCY 3 cut(s) 585, 1281, 1591
MhlI GDGCHC 1 cut(s) 855
MlsI TGGCCA 1 cut(s) 1238
MluCI AATT 6 cut(s) 730, 824, 970, 1021, 1116, 1547
MluNI TGGCCA 1 cut(s) 1238
MlyI GAGTC 2 cut(s) 582, 1070
Mox20I TGGCCA 1 cut(s) 1238
Mph1103I ATGCAT 2 cut(s) 288, 661
MroXI GAANNNNTTC 1 cut(s) 947
MscI TGGCCA 1 cut(s) 1238
MseI TTAA 8 cut(s) 171, 192, 236, 255, 332, 1103, 1365, 1578
MslI CAYNNNNRTG 1 cut(s) 1705
Msp20I TGGCCA 1 cut(s) 1238
MspA1I CMGCKG 1 cut(s) 1400
MspCI CTTAAG 2 cut(s) 235, 1102
MspI CCGG 3 cut(s) 695, 986, 1440
MspR9I CCNGG 3 cut(s) 214, 1441, 1657
Mva1269I GAATGC 1 cut(s) 1154
MvaI CCWGG 2 cut(s) 214, 1657
MwoI GCNNNNNNNGC 7 cut(s) 479, 660, 902, 1244, 1359, 1486, 1572
NciI CCSGG 1 cut(s) 1441
NdeI CATATG 1 cut(s) 1459
NlaIV GGNNCC 1 cut(s) 1392
NmuCI GTSAC 2 cut(s) 644, 1568
NsiI ATGCAT 2 cut(s) 288, 661
NspI RCATGY 2 cut(s) 317, 663
OliI CACNNNNGTG 1 cut(s) 1705
PaeI GCATGC 1 cut(s) 663
PaeR7I CTCGAG 2 cut(s) 625, 1583
PagI TCATGA 1 cut(s) 199
PctI GAATGC 1 cut(s) 1154
PdmI GAANNNNTTC 1 cut(s) 947
PfeI GAWTC 8 cut(s) 8, 21, 177, 203, 298, 376, 613, 1083
PflMI CCANNNNNTGG 2 cut(s) 1387, 1496
PkrI GCNGC 2 cut(s) 1220, 1488
PleI GAGTC 2 cut(s) 581, 1069
PpsI GAGTC 2 cut(s) 581, 1069
PshAI GACNNNNGTC 1 cut(s) 865
Psp124BI GAGCTC 1 cut(s) 855
Psp1406I AACGTT 1 cut(s) 1542
Psp6I CCWGG 2 cut(s) 212, 1655
PspFI CCCAGC 1 cut(s) 1713
PspGI CCWGG 2 cut(s) 212, 1655
PspN4I GGNNCC 1 cut(s) 1392
PstNI CAGNNNCTG 2 cut(s) 391, 1572
PsuI RGATCY 3 cut(s) 585, 1281, 1591
RsaI GTAC 3 cut(s) 818, 1051, 1108
RsaNI GTAC 3 cut(s) 817, 1050, 1107
RseI CAYNNNNRTG 1 cut(s) 1705
SacI GAGCTC 1 cut(s) 855
SaqAI TTAA 8 cut(s) 171, 192, 236, 255, 332, 1103, 1365, 1578
SatI GCNGC 2 cut(s) 1219, 1487
SchI GAGTC 2 cut(s) 582, 1070
ScrFI CCNGG 3 cut(s) 214, 1441, 1657
SduI GDGCHC 1 cut(s) 855
SfaNI GCATC 8 cut(s) 589, 757, 883, 925, 948, 1473, 1621, 1642
SfcI CTRYAG 1 cut(s) 1596
Sfr274I CTCGAG 2 cut(s) 625, 1583
SlaI CTCGAG 2 cut(s) 625, 1583
SmiMI CAYNNNNRTG 1 cut(s) 1705
SmlI CTYRAG 6 cut(s) 182, 235, 625, 1003, 1102, 1583
SmoI CTYRAG 6 cut(s) 182, 235, 625, 1003, 1102, 1583
SphI GCATGC 1 cut(s) 663
Sse9I AATT 6 cut(s) 730, 824, 970, 1021, 1116, 1547
SsiI CCGC 6 cut(s) 88, 549, 720, 1137, 1221, 1398
SspI AATATT 1 cut(s) 157
SspMI CTAG 5 cut(s) 150, 450, 557, 749, 1448
SstI GAGCTC 1 cut(s) 855
StyD4I CCNGG 3 cut(s) 212, 1439, 1655
StyI CCWWGG 1 cut(s) 1149
TaaI ACNGT 9 cut(s) 75, 265, 511, 1029, 1192, 1253, 1663, 1673, 1692
TaiI ACGT 2 cut(s) 273, 1545
TaqI TCGA 4 cut(s) 626, 765, 1092, 1584
TasI AATT 6 cut(s) 730, 824, 970, 1021, 1116, 1547
TfiI GAWTC 8 cut(s) 8, 21, 177, 203, 298, 376, 613, 1083
Tru1I TTAA 8 cut(s) 171, 192, 236, 255, 332, 1103, 1365, 1578
Tru9I TTAA 8 cut(s) 171, 192, 236, 255, 332, 1103, 1365, 1578
TscAI CASTG 3 cut(s) 333, 1531, 1577
TseFI GTSAC 2 cut(s) 644, 1568
TseI GCWGC 2 cut(s) 1218, 1486
Tsp45I GTSAC 2 cut(s) 644, 1568
TspDTI ATGAA 7 cut(s) 56, 368, 758, 963, 1145, 1388, 1716
TspRI CASTG 3 cut(s) 333, 1531, 1577
Van91I CCANNNNNTGG 2 cut(s) 1387, 1496
Vha464I CTTAAG 2 cut(s) 235, 1102
XapI RAATTY 2 cut(s) 970, 1547
XbaI TCTAGA 1 cut(s) 449
XceI RCATGY 2 cut(s) 317, 663
XhoI CTCGAG 2 cut(s) 625, 1583
XmnI GAANNNNTTC 1 cut(s) 947
XspI CTAG 5 cut(s) 150, 450, 557, 749, 1448
Zsp2I ATGCAT 2 cut(s) 288, 661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.