Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0020196

Physical Location & Seq

Genomic Coordinates Reverse (-)

10696606 .. 10698342

Gene Structure 1737 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1078100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1737 bp

ATGAATCCAAATGGTCAAAATCTTAGATTTCTACTGGAGAAATCTCTAACCCTGAAGCAACTCAAACAAGTTCACGCACCCATTATATCCAATGGCCTCCACCACCTCCAAATCCTCCTGCTCCGCCAAGTCCTCCACTCAGATTTCACCTTCTCTGCAGCTACAGCCCGCTACGTGCACCTAATCCTTCACAATCTCAAAAACCCAGATGCATTTTCATGGAGCTGCGCCATTCGTTACTTCTCTCTGCATTCCCAGTACGAAACGGCTTTCTCTCTTTACCTTAAGATAAAAAGGCTCGGACTTTGCCCATCTAGTTTTGCTGTGTCCTCTGCTCTGAGGGCGTGTGCAAGGATTTTGAATGAATTGGGTGGGATATTGATCCATGCCCAGGTTTATAAATTTGGGTTTTGTGGGTGTGTTTATGTACAAACTGCGCTCGTTGATTTTTATTCGAAACTCGGTGACATGAAAACTGCACGGAAGGTGTTTGATGGAATGCCTGAGAAGAATGTTGTTTCTTGGAATTCTGTTTTGTCCGGGTATATGAAATCTGGGGATATGGAGGAGGCTCAGGAGGTGTTTGATGAGATTCCAAATAAGGATGTTGTGTCTTGGAATTCCATGGTTTCGGGGTATGCGAGGATGGGAAATATGGACAAGGCATATTTCTTGTTTCAACAAATTCCACAGAGGAGTTTGGCTTCTTGGAATGCAATGATTAGTGGTTATGTGGAATGTGAAAGAGTGGAATCGGCTAGAGTCATTTTTGATGCCATGCCGTTAAGAAATAATGTTTCTTGGATCACGATGATTAGCGGGTACTCAAAATGCGGCAATGTTGAGTCCGCTCGTCAACTCTTTGATCAGATGAGTGAAAGAGATGTGCTCTTGTTCAATGCCATGATAGCTTGCTATGCACAAAATAGCCAACCAAAGGAGGCCATTGAACTGTTCAACCAGATGCTTAAACTGGAAGTGAATATTCAACCAAATGAGATGACTTTGGCTAGTGTTATATCTGCTAGTTCTCAACTGGGAGATATGAAATTTGGGTTGTGGGCTGAGTTCTATATGAGTGAGCACGGAATTGAATTAGATGATCATTTGGCTACCGCTTTAGTTGATTTGTACACAAAATGTGGAGACATTGATAGGGCATATAAGTTGTTTCACGGCTTGAAAAAGAGAGATGTAGTTGCATATTCTGCAATGATCTTGGGATGCGGCATAAATGGTAAGGCAGTCGAAGCAACCAAGTTGTTTGAAGAGATGGTAAATGCCGACATTTGCCCCAATGTGGTAACATACACTGGACTGCTAACTGCCTATAAGCATGCCGGTCTAGTTGAAGAAGGCTACCGATGCTTTAATTCCATGAACGAACATGGACTTGAGCCATCGGGCGATCATTATGGAATCATGGTCGACCTTTTAGGCAGAGCAGGACAGTTGGACAAAGCAGTTGAGCTAATAAGAAGCATGCCAATGCAGCCTCAAGCGGGGGTTTGGGGAGCTTTGCTTCTTGCTTGCTCATTACACAACAATGTTGAGCTTGGGGAGACTGCTGCTCGGAATTGTTTTAAGTTGGAGCCTGATAAAAGCGGTTATTACGCTCTTCTTGCCAACATTTATGCTTCTGCTGCGAGATGGGACGATGAGAGGAGATTGAGAAAGGTCATGGAGAAGAAGGGGTTCGCCAAGATTCCAGGCTGTAGCTGGATGGATTCCATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR

Protein Analysis

579

Amino Acids

64.85

Weight (kDa)

6.58

Isoelectric Point (pI)

38.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	170 - 200	3.1e-07	PPR repeat family
PPR	PF01535	173 - 201	1.3e-07	PPR repeat
PPR	PF01535	204 - 232	1.4e-06	PPR repeat
PPR_2	PF13041	264 - 296	1e-06	PPR repeat family
PPR	PF01535	266 - 295	1.1e-07	PPR repeat
PPR_long	PF17177	281 - 396	6.7e-07	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	295 - 342	3.1e-08	PPR repeat family
PPR	PF01535	299 - 325	1.8e-06	PPR repeat
PPR_2	PF13041	397 - 444	2.6e-11	PPR repeat family
PPR_long	PF17177	401 - 492	2.6e-06	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	420 - 480	7.5e-09	Pentatricopeptide repeat domain
PPR_2	PF13041	432 - 477	1.6e-07	PPR repeat family
E_motif	PF20431	514 - 575	7.4e-19	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0020196)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G22760
malus_domestica	MD04G1078100.v1.1
prunus_persica	Prupe.5G080600_v2.0.a1
pyrus_communis	pycom04g07010 pycom06g06320 pycom06g06340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	399
AccBSI	CCGCTC	1 cut(s)	851
AccI	GTMKAC	1 cut(s)	1428
AciI	CCGC	9 cut(s)	124, 169, 819, 834, 849, 1116, 1227, 1502, 1605
AclWI	GGATC	2 cut(s)	376, 812
AcsI	RAATTY	5 cut(s)	401, 526, 619, 684, 1049
AcuI	CTGAAG	1 cut(s)	74
AfaI	GTAC	4 cut(s)	260, 429, 824, 1133
AfiI	CCNNNNNNNGG	4 cut(s)	391, 937, 1300, 1502
AflII	CTTAAG	1 cut(s)	284
AjnI	CCWGG	2 cut(s)	390, 1708
AluBI	AGCT	7 cut(s)	161, 225, 911, 1471, 1517, 1555, 1719
AluI	AGCT	7 cut(s)	161, 225, 911, 1471, 1517, 1555, 1719
Alw21I	GWGCWC	3 cut(s)	180, 891, 1086
Alw26I	GTCTC	2 cut(s)	1140, 1556
Alw44I	GTGCAC	1 cut(s)	176
AlwI	GGATC	2 cut(s)	376, 812
AoxI	GGCC	2 cut(s)	94, 942
ApaLI	GTGCAC	1 cut(s)	176
ApeKI	GCWGC	5 cut(s)	158, 225, 1492, 1568, 1643
ApoI	RAATTY	5 cut(s)	401, 526, 619, 684, 1049
Asp700I	GAANNNNTTC	1 cut(s)	1694
AspLEI	GCGC	2 cut(s)	230, 439
AsuC2I	CCSGG	1 cut(s)	541
AsuHPI	GGTGA	2 cut(s)	139, 476
AsuII	TTCGAA	1 cut(s)	455
BaeGI	GKGCMC	1 cut(s)	180
Bbv12I	GWGCWC	3 cut(s)	180, 891, 1086
BbvI	GCAGC	5 cut(s)	170, 212, 1504, 1555, 1630
BccI	CCATC	7 cut(s)	319, 488, 640, 1267, 1408, 1644, 1717
BceAI	ACGGC	3 cut(s)	282, 766, 1192
BciT130I	CCWGG	2 cut(s)	392, 1710
BclI	TGATCA	2 cut(s)	865, 1102
BcnI	CCSGG	1 cut(s)	541
BcoDI	GTCTC	2 cut(s)	1140, 1556
BfaI	CTAG	5 cut(s)	315, 759, 1011, 1026, 1346
BfmI	CTRYAG	3 cut(s)	156, 162, 1714
BfrI	CTTAAG	1 cut(s)	284
BisI	GCNGC	7 cut(s)	159, 226, 835, 1228, 1493, 1569, 1644
BlsI	GCNGC	7 cut(s)	160, 227, 836, 1229, 1494, 1570, 1645
Bme1390I	CCNGG	3 cut(s)	392, 541, 1710
BmiI	GGNNCC	1 cut(s)	1593
BmrFI	CCNGG	3 cut(s)	392, 541, 1710
BmrI	ACTGGG	2 cut(s)	250, 1046
BmsI	GCATC	5 cut(s)	199, 763, 954, 1214, 1355
BmuI	ACTGGG	2 cut(s)	250, 1046
BplI	GAGNNNNNCTC	2 cut(s)	873, 905
BpmI	CTGGAG	1 cut(s)	56
Bpu10I	CCTNAGC	1 cut(s)	573
Bpu14I	TTCGAA	1 cut(s)	455
BpuEI	CTTGAG	2 cut(s)	1415, 1482
BpuMI	CCSGG	1 cut(s)	541
BsaAI	YACGTR	1 cut(s)	175
BsaBI	GATNNNNATC	1 cut(s)	380
BsaJI	CCNNGG	2 cut(s)	390, 624
Bsc4I	CCNNNNNNNGG	4 cut(s)	391, 937, 1300, 1502
Bse118I	RCCGGY	1 cut(s)	1340
Bse1I	ACTGG	5 cut(s)	39, 256, 978, 1041, 1318
Bse3DI	GCAATG	3 cut(s)	723, 844, 1218
Bse8I	GATNNNNATC	1 cut(s)	380
BseBI	CCWGG	2 cut(s)	392, 1710
BseDI	CCNNGG	2 cut(s)	390, 624
BseGI	GGATG	4 cut(s)	610, 651, 1229, 1728
BseJI	GATNNNNATC	1 cut(s)	380
BseLI	CCNNNNNNNGG	4 cut(s)	391, 937, 1300, 1502
BseMI	GCAATG	3 cut(s)	723, 844, 1218
BseMII	CTCAG	5 cut(s)	153, 329, 495, 587, 1056
BseNI	ACTGG	5 cut(s)	39, 256, 978, 1041, 1318
BseRI	GAGGAG	3 cut(s)	581, 709, 1678
BseSI	GKGCMC	1 cut(s)	180
BseXI	GCAGC	5 cut(s)	170, 212, 1504, 1555, 1630
BsgI	GTGCAG	1 cut(s)	462
BshFI	GGCC	2 cut(s)	96, 944
BsiHKAI	GWGCWC	3 cut(s)	180, 891, 1086
BsiSI	CCGG	2 cut(s)	540, 1341
BslFI	GGGAC	1 cut(s)	1667
BslI	CCNNNNNNNGG	4 cut(s)	391, 937, 1300, 1502
BsmAI	GTCTC	2 cut(s)	1140, 1556
BsmFI	GGGAC	1 cut(s)	1667
BsmI	GAATGC	3 cut(s)	250, 504, 718
BsnI	GGCC	2 cut(s)	96, 944
Bsp119I	TTCGAA	1 cut(s)	455
Bsp1286I	GDGCHC	3 cut(s)	180, 891, 1086
Bsp1407I	TGTACA	2 cut(s)	427, 1131
Bsp143I	GATC	6 cut(s)	381, 804, 865, 1102, 1215, 1408
Bsp19I	CCATGG	1 cut(s)	624
BspACI	CCGC	9 cut(s)	124, 169, 819, 834, 849, 1116, 1227, 1502, 1605
BspANI	GGCC	2 cut(s)	96, 944
BspCNI	CTCAG	5 cut(s)	152, 330, 496, 586, 1057
BspLI	GGNNCC	1 cut(s)	1593
BspMAI	CTGCAG	1 cut(s)	160
BspPI	GGATC	2 cut(s)	376, 812
BspQI	GCTCTTC	1 cut(s)	1623
BspT104I	TTCGAA	1 cut(s)	455
BspTI	CTTAAG	1 cut(s)	284
BsrBI	CCGCTC	1 cut(s)	851
BsrDI	GCAATG	3 cut(s)	723, 844, 1218
BsrFI	RCCGGY	1 cut(s)	1340
BsrGI	TGTACA	2 cut(s)	427, 1131
BsrI	ACTGG	5 cut(s)	39, 256, 978, 1041, 1318
BssAI	RCCGGY	1 cut(s)	1340
BssECI	CCNNGG	2 cut(s)	390, 624
BssMI	GATC	6 cut(s)	381, 804, 865, 1102, 1215, 1408
BssT1I	CCWWGG	1 cut(s)	624
Bst2UI	CCWGG	2 cut(s)	392, 1710
Bst4CI	ACNGT	2 cut(s)	954, 1452
Bst6I	CTCTTC	2 cut(s)	1263, 1623
BstAFI	CTTAAG	1 cut(s)	284
BstAPI	GCANNNNNTGC	1 cut(s)	1208
BstAUI	TGTACA	2 cut(s)	427, 1131
BstBAI	YACGTR	1 cut(s)	175
BstBI	TTCGAA	1 cut(s)	455
BstC8I	GCNNGC	5 cut(s)	169, 913, 1338, 1484, 1531
BstDEI	CTNAG	6 cut(s)	23, 139, 338, 504, 573, 1065
BstDSI	CCRYGG	1 cut(s)	624
BstF5I	GGATG	4 cut(s)	610, 651, 1229, 1728
BstHHI	GCGC	2 cut(s)	230, 439
BstKTI	GATC	6 cut(s)	384, 807, 868, 1105, 1218, 1411
BstMAI	GTCTC	2 cut(s)	1140, 1556
BstMBI	GATC	6 cut(s)	381, 804, 865, 1102, 1215, 1408
BstNI	CCWGG	2 cut(s)	392, 1710
BstNSI	RCATGY	2 cut(s)	1340, 1486
BstSCI	CCNGG	3 cut(s)	390, 539, 1708
BstSFI	CTRYAG	3 cut(s)	156, 162, 1714
BstSLI	GKGCMC	1 cut(s)	180
BstV1I	GCAGC	5 cut(s)	170, 212, 1504, 1555, 1630
BsuRI	GGCC	2 cut(s)	96, 944
BtgI	CCRYGG	1 cut(s)	624
BtsCI	GGATG	4 cut(s)	610, 651, 1229, 1728
BtsIMutI	CAGTG	1 cut(s)	1311
Cac8I	GCNNGC	5 cut(s)	169, 913, 1338, 1484, 1531
CfoI	GCGC	2 cut(s)	230, 439
Cfr10I	RCCGGY	1 cut(s)	1340
Csp6I	GTAC	4 cut(s)	259, 428, 823, 1132
CviQI	GTAC	4 cut(s)	259, 428, 823, 1132
DdeI	CTNAG	6 cut(s)	23, 139, 338, 504, 573, 1065
DpnI	GATC	6 cut(s)	383, 806, 867, 1104, 1217, 1410
DpnII	GATC	6 cut(s)	381, 804, 865, 1102, 1215, 1408
Eam1104I	CTCTTC	2 cut(s)	1263, 1623
EarI	CTCTTC	2 cut(s)	1263, 1623
EciI	GGCGGA	1 cut(s)	113
Eco130I	CCWWGG	1 cut(s)	624
Eco57I	CTGAAG	1 cut(s)	74
EcoRI	GAATTC	2 cut(s)	526, 619
EcoRII	CCWGG	2 cut(s)	390, 1708
EcoT14I	CCWWGG	1 cut(s)	624
EcoT22I	ATGCAT	1 cut(s)	214
ErhI	CCWWGG	1 cut(s)	624
FaqI	GGGAC	1 cut(s)	1667
FauI	CCCGC	3 cut(s)	176, 812, 1495
FbaI	TGATCA	2 cut(s)	865, 1102
FblI	GTMKAC	1 cut(s)	1428
Fnu4HI	GCNGC	7 cut(s)	159, 226, 835, 1228, 1493, 1569, 1644
FokI	GGATG	3 cut(s)	617, 658, 1236
Fsp4HI	GCNGC	7 cut(s)	159, 226, 835, 1228, 1493, 1569, 1644
FspBI	CTAG	5 cut(s)	315, 759, 1011, 1026, 1346
GlaI	GCGC	2 cut(s)	229, 438
GluI	GCNGC	7 cut(s)	159, 226, 835, 1228, 1493, 1569, 1644
GsuI	CTGGAG	1 cut(s)	56
HaeIII	GGCC	2 cut(s)	96, 944
HapII	CCGG	2 cut(s)	540, 1341
HhaI	GCGC	2 cut(s)	230, 439
Hin6I	GCGC	2 cut(s)	228, 437
HinP1I	GCGC	2 cut(s)	228, 437
HincII	GTYRAC	2 cut(s)	857, 1429
HindII	GTYRAC	2 cut(s)	857, 1429
HinfI	GANTC	8 cut(s)	4, 592, 752, 762, 845, 1419, 1705, 1727
HpaII	CCGG	2 cut(s)	540, 1341
HphI	GGTGA	2 cut(s)	139, 476
Hpy166II	GTNNAC	5 cut(s)	73, 178, 857, 1134, 1429
Hpy188I	TCNGA	5 cut(s)	142, 302, 339, 870, 1575
Hpy188III	TCNNGA	2 cut(s)	575, 808
Hpy8I	GTNNAC	5 cut(s)	73, 178, 857, 1134, 1429
HpyAV	CCTTC	5 cut(s)	160, 197, 478, 1349, 1684
HpyCH4III	ACNGT	2 cut(s)	954, 1452
HpyCH4IV	ACGT	1 cut(s)	174
HpyF3I	CTNAG	6 cut(s)	23, 139, 338, 504, 573, 1065
HpySE526I	ACGT	1 cut(s)	174
HspAI	GCGC	2 cut(s)	228, 437
Ksp22I	TGATCA	2 cut(s)	865, 1102
Kzo9I	GATC	6 cut(s)	381, 804, 865, 1102, 1215, 1408
LguI	GCTCTTC	1 cut(s)	1623
LmnI	GCTCC	4 cut(s)	126, 222, 1514, 1591
Lsp1109I	GCAGC	5 cut(s)	170, 212, 1504, 1555, 1630
LweI	GCATC	5 cut(s)	199, 763, 954, 1214, 1355
MaeI	CTAG	5 cut(s)	315, 759, 1011, 1026, 1346
MaeII	ACGT	1 cut(s)	174
MaeIII	GTNAC	3 cut(s)	236, 464, 1303
MalI	GATC	6 cut(s)	383, 806, 867, 1104, 1217, 1410
MbiI	CCGCTC	1 cut(s)	851
MboI	GATC	6 cut(s)	381, 804, 865, 1102, 1215, 1408
MboII	GAAGA	5 cut(s)	520, 1280, 1364, 1610, 1699
MhlI	GDGCHC	3 cut(s)	180, 891, 1086
MlyI	GAGTC	2 cut(s)	771, 854
MmeI	TCCRAC	2 cut(s)	1434, 1569
Mph1103I	ATGCAT	1 cut(s)	214
MroXI	GAANNNNTTC	1 cut(s)	1694
MseI	TTAA	5 cut(s)	285, 785, 969, 1371, 1584
MslI	CAYNNNNRTG	3 cut(s)	217, 1487, 1545
MspCI	CTTAAG	1 cut(s)	284
MspI	CCGG	2 cut(s)	540, 1341
MspR9I	CCNGG	3 cut(s)	392, 541, 1710
Mva1269I	GAATGC	3 cut(s)	250, 504, 718
MvaI	CCWGG	2 cut(s)	392, 1710
NciI	CCSGG	1 cut(s)	541
NcoI	CCATGG	1 cut(s)	624
NdeII	GATC	6 cut(s)	381, 804, 865, 1102, 1215, 1408
NlaIV	GGNNCC	1 cut(s)	1593
NmuCI	GTSAC	1 cut(s)	464
NsiI	ATGCAT	1 cut(s)	214
NspI	RCATGY	2 cut(s)	1340, 1486
NspV	TTCGAA	1 cut(s)	455
PaeI	GCATGC	2 cut(s)	1340, 1486
PciSI	GCTCTTC	1 cut(s)	1623
PcsI	WCGNNNNNNNCGW	1 cut(s)	638
PctI	GAATGC	3 cut(s)	250, 504, 718
PdmI	GAANNNNTTC	1 cut(s)	1694
PfeI	GAWTC	6 cut(s)	4, 592, 752, 1419, 1705, 1727
PkrI	GCNGC	7 cut(s)	160, 227, 836, 1229, 1494, 1570, 1645
PleI	GAGTC	2 cut(s)	770, 853
PpsI	GAGTC	2 cut(s)	770, 853
Ppu21I	YACGTR	1 cut(s)	175
PsiI	TTATAA	1 cut(s)	399
Psp6I	CCWGG	2 cut(s)	390, 1708
PspGI	CCWGG	2 cut(s)	390, 1708
PspN4I	GGNNCC	1 cut(s)	1593
PstI	CTGCAG	1 cut(s)	160
RsaI	GTAC	4 cut(s)	260, 429, 824, 1133
RsaNI	GTAC	4 cut(s)	259, 428, 823, 1132
RseI	CAYNNNNRTG	3 cut(s)	217, 1487, 1545
SalI	GTCGAC	1 cut(s)	1427
SapI	GCTCTTC	1 cut(s)	1623
SaqAI	TTAA	5 cut(s)	285, 785, 969, 1371, 1584
SatI	GCNGC	7 cut(s)	159, 226, 835, 1228, 1493, 1569, 1644
Sau3AI	GATC	6 cut(s)	381, 804, 865, 1102, 1215, 1408
SchI	GAGTC	2 cut(s)	771, 854
ScrFI	CCNGG	3 cut(s)	392, 541, 1710
SduI	GDGCHC	3 cut(s)	180, 891, 1086
SfaNI	GCATC	5 cut(s)	199, 763, 954, 1214, 1355
SfcI	CTRYAG	3 cut(s)	156, 162, 1714
SfuI	TTCGAA	1 cut(s)	455
SmiMI	CAYNNNNRTG	3 cut(s)	217, 1487, 1545
SmlI	CTYRAG	3 cut(s)	284, 1394, 1497
SmoI	CTYRAG	3 cut(s)	284, 1394, 1497
SphI	GCATGC	2 cut(s)	1340, 1486
SsiI	CCGC	9 cut(s)	124, 169, 819, 834, 849, 1116, 1227, 1502, 1605
SspI	AATATT	1 cut(s)	985
SspMI	CTAG	5 cut(s)	315, 759, 1011, 1026, 1346
StyD4I	CCNGG	3 cut(s)	390, 539, 1708
StyI	CCWWGG	1 cut(s)	624
TaaI	ACNGT	2 cut(s)	954, 1452
TaiI	ACGT	1 cut(s)	177
TaqI	TCGA	3 cut(s)	455, 1248, 1428
TatI	WGTACW	2 cut(s)	427, 1131
TauI	GCSGC	2 cut(s)	837, 1230
TfiI	GAWTC	6 cut(s)	4, 592, 752, 1419, 1705, 1727
Tru1I	TTAA	5 cut(s)	285, 785, 969, 1371, 1584
Tru9I	TTAA	5 cut(s)	285, 785, 969, 1371, 1584
TscAI	CASTG	1 cut(s)	1318
TseFI	GTSAC	1 cut(s)	464
TseI	GCWGC	5 cut(s)	158, 225, 1492, 1568, 1643
Tsp45I	GTSAC	1 cut(s)	464
TspDTI	ATGAA	7 cut(s)	17, 207, 378, 485, 563, 1061, 1394
TspGWI	ACGGA	2 cut(s)	496, 1101
TspRI	CASTG	1 cut(s)	1318
Vha464I	CTTAAG	1 cut(s)	284
VneI	GTGCAC	1 cut(s)	176
XapI	RAATTY	5 cut(s)	401, 526, 619, 684, 1049
XceI	RCATGY	2 cut(s)	1340, 1486
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1716
XmiI	GTMKAC	1 cut(s)	1428
XmnI	GAANNNNTTC	1 cut(s)	1694
XspI	CTAG	5 cut(s)	315, 759, 1011, 1026, 1346
Zsp2I	ATGCAT	1 cut(s)	214

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.