AT4G26860

Pyridoxal 5'-phosphate (PLP)-binding protein, which may be involved in intracellular homeostatic regulation of pyridoxal 5'-phosphate (PLP), the active form of vitamin B6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0017509
Physical Location & Seq
Reverse (-)
13503053 .. 13504876
1824 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G26860.1

Sequence Viewer

Length: 735 bp
ATGGCAGCTCCTGCGGTGGAGGCAACCGTCGCAAGTGCTCTCCGCTCTGTGATTCTTAGAGCTCGTAAGGCGGCGGAGCAAGTAGGAAGAGATCCCGAGCGGGTCAGGGTCCTTCCAGTCTCCAAGACTAAACCCGTCTCACTTATCCGCCAAATCTACGACGCAGGTCACCGTTGCTTCGGCGAGAATTACGTTCAAGAAATCATCGATAAAGCTCCACAGCTTCCGGAAGATATAGAGTGGCATTTCGTGGGGCATTTACAGAGCAACAAAGCCAAAACGCTATTGACTGGAGTCCCAAATTTGGCAATGGTTCATGGTGTGGATGGAGAAAAGGTAGCAAATCATCTAGATCGAGCTGTTTCAAATCTTGGGAGACATCCACTAAAGGTTTTGGTCCAAGTGAACACAAGCGGAGAAGTTTCTAAATCTGGAATCGAACCTTCTAGCGTTGTAGAGCTAGCAAGACATGTGAAACATCACTGTCCAAATCTGGTATTCTCCGGATTAATGACTATTGGGATGCCTGATTATACGTCTACCCCAGAGAACTTCAGGACACTTTCAAACTGTAGAGCTGATGTATGCAAGGCACTTGGAATGGCTGAGGATCAATTTGAACTGTCCATGGGCATGTCTGGTGACTTCGAGCTAGCGATTGAGATGGGAAGCACCAATGTGAGGGTTGGTTCCACCATTTTCGGACCAAGAGAGTACCCCAAGAAAACAACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ala_racemase_N

Protein Analysis

244

Amino Acids

26.64

Weight (kDa)

7.75

Isoelectric Point (pI)

38.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ala_racemase_N PF01168 34 - 236 6.3e-15 Alanine racemase, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017509)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26860 AT4G26860
fragaria_vesca FvH4_3g23210
malus_domestica MD03G1196600.v1.1
prunus_persica Prupe.4G218800_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0040871
rosa_laevigata RLG00000034010
rosa_roxburghii Rroxscaffold_1G00039830
rosa_rugosa Rorug05G0188200
rosa_samantha Rh5AG273900 Rh5BG278900
rosa_wichuraiana Rw5G025740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 155
AccBSI CCGCTC 2 cut(s) 45, 100
AccI GTMKAC 1 cut(s) 539
AccIII TCCGGA 2 cut(s) 226, 503
AciI CCGC 7 cut(s) 14, 43, 71, 74, 100, 148, 414
AclWI GGATC 2 cut(s) 86, 618
AcsI RAATTY 1 cut(s) 301
AcuI CTGAAG 1 cut(s) 538
AfaI GTAC 1 cut(s) 716
AfiI CCNNNNNNNGG 2 cut(s) 304, 681
AflIII ACRYGT 1 cut(s) 469
AgsI TTSAA 4 cut(s) 197, 366, 567, 620
AleI CACNNNNGTG 1 cut(s) 677
AluBI AGCT 8 cut(s) 8, 62, 215, 223, 359, 460, 578, 652
AluI AGCT 8 cut(s) 8, 62, 215, 223, 359, 460, 578, 652
Alw21I GWGCWC 2 cut(s) 40, 64
Alw26I GTCTC 3 cut(s) 124, 142, 370
AlwI GGATC 2 cut(s) 86, 618
AlwNI CAGNNNCTG 1 cut(s) 11
Ama87I CYCGRG 1 cut(s) 95
Aor13HI TCCGGA 2 cut(s) 226, 503
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 1 cut(s) 301
AseI ATTAAT 1 cut(s) 509
AspS9I GGNCC 3 cut(s) 109, 397, 704
AsuHPI GGTGA 2 cut(s) 161, 653
AsuNHI GCTAGC 2 cut(s) 460, 652
AvaI CYCGRG 1 cut(s) 95
AvaII GGWCC 3 cut(s) 109, 397, 704
BanII GRGCYC 1 cut(s) 64
Bbv12I GWGCWC 2 cut(s) 40, 64
BbvCI CCTCAGC 1 cut(s) 606
BbvI GCAGC 1 cut(s) 17
BccI CCATC 2 cut(s) 320, 658
BcoDI GTCTC 3 cut(s) 124, 142, 370
BfaI CTAG 4 cut(s) 350, 447, 461, 653
BfmI CTRYAG 1 cut(s) 571
BfuAI ACCTGC 1 cut(s) 155
BisI GCNGC 2 cut(s) 6, 72
BlsI GCNGC 2 cut(s) 7, 73
Bme18I GGWCC 3 cut(s) 109, 397, 704
BmeT110I CYCGRG 1 cut(s) 95
BmgT120I GGNCC 3 cut(s) 109, 397, 704
BmiI GGNNCC 2 cut(s) 110, 691
BmsI GCATC 1 cut(s) 513
BmtI GCTAGC 2 cut(s) 464, 656
BoxI GACNNNNGTC 2 cut(s) 165, 293
BpmI CTGGAG 1 cut(s) 312
Bpu10I CCTNAGC 1 cut(s) 606
Bsa29I ATCGAT 1 cut(s) 207
BsaJI CCNNGG 1 cut(s) 627
BsaWI WCCGGW 2 cut(s) 226, 503
BsaXI ACNNNNNCTCC 2 cut(s) 321, 351
Bsc4I CCNNNNNNNGG 2 cut(s) 304, 681
Bse1I ACTGG 2 cut(s) 116, 295
Bse3DI GCAATG 1 cut(s) 315
BseAI TCCGGA 2 cut(s) 226, 503
BseCI ATCGAT 1 cut(s) 207
BseDI CCNNGG 1 cut(s) 627
BseGI GGATG 3 cut(s) 331, 379, 528
BseLI CCNNNNNNNGG 2 cut(s) 304, 681
BseMI GCAATG 1 cut(s) 315
BseMII CTCAG 1 cut(s) 597
BseNI ACTGG 2 cut(s) 116, 295
BseXI GCAGC 1 cut(s) 17
BshVI ATCGAT 1 cut(s) 207
BsiHKAI GWGCWC 2 cut(s) 40, 64
BsiHKCI CYCGRG 1 cut(s) 95
BsiSI CCGG 2 cut(s) 227, 504
BslFI GGGAC 1 cut(s) 281
BslI CCNNNNNNNGG 2 cut(s) 304, 681
BsmAI GTCTC 3 cut(s) 124, 142, 370
BsmBI CGTCTC 1 cut(s) 142
BsmFI GGGAC 1 cut(s) 281
BsoBI CYCGRG 1 cut(s) 95
Bsp1286I GDGCHC 2 cut(s) 40, 64
Bsp13I TCCGGA 2 cut(s) 226, 503
Bsp143I GATC 3 cut(s) 91, 352, 610
Bsp19I CCATGG 1 cut(s) 627
BspACI CCGC 7 cut(s) 14, 43, 71, 74, 100, 148, 414
BspCNI CTCAG 1 cut(s) 598
BspDI ATCGAT 1 cut(s) 207
BspEI TCCGGA 2 cut(s) 226, 503
BspLI GGNNCC 2 cut(s) 110, 691
BspMI ACCTGC 1 cut(s) 155
BspOI GCTAGC 2 cut(s) 464, 656
BspPI GGATC 2 cut(s) 86, 618
BsrBI CCGCTC 2 cut(s) 45, 100
BsrDI GCAATG 1 cut(s) 315
BsrI ACTGG 2 cut(s) 116, 295
BssECI CCNNGG 1 cut(s) 627
BssMI GATC 3 cut(s) 91, 352, 610
BssT1I CCWWGG 1 cut(s) 627
Bst4CI ACNGT 5 cut(s) 28, 173, 485, 572, 624
Bst6I CTCTTC 1 cut(s) 82
BstAPI GCANNNNNTGC 1 cut(s) 11
BstC8I GCNNGC 2 cut(s) 462, 654
BstDEI CTNAG 3 cut(s) 56, 606, 732
BstDSI CCRYGG 1 cut(s) 627
BstEII GGTNACC 1 cut(s) 167
BstF5I GGATG 3 cut(s) 331, 379, 528
BstKTI GATC 3 cut(s) 94, 355, 613
BstMAI GTCTC 3 cut(s) 124, 142, 370
BstMBI GATC 3 cut(s) 91, 352, 610
BstMWI GCNNNNNNNGC 4 cut(s) 11, 20, 29, 68
BstNSI RCATGY 2 cut(s) 473, 637
BstPAI GACNNNNGTC 2 cut(s) 165, 293
BstPI GGTNACC 1 cut(s) 167
BstSFI CTRYAG 1 cut(s) 571
BstV1I GCAGC 1 cut(s) 17
BstX2I RGATCY 1 cut(s) 91
BstYI RGATCY 1 cut(s) 91
Bsu15I ATCGAT 1 cut(s) 207
BsuTUI ATCGAT 1 cut(s) 207
BtgI CCRYGG 1 cut(s) 627
BtsCI GGATG 3 cut(s) 331, 379, 528
BtsIMutI CAGTG 1 cut(s) 481
BveI ACCTGC 1 cut(s) 155
Cac8I GCNNGC 2 cut(s) 462, 654
CaiI CAGNNNCTG 1 cut(s) 11
Cfr13I GGNCC 3 cut(s) 109, 397, 704
ClaI ATCGAT 1 cut(s) 207
CseI GACGC 1 cut(s) 170
Csp6I GTAC 1 cut(s) 715
CviAII CATG 4 cut(s) 317, 470, 628, 634
CviQI GTAC 1 cut(s) 715
DdeI CTNAG 3 cut(s) 56, 606, 732
DpnI GATC 3 cut(s) 93, 354, 612
DpnII GATC 3 cut(s) 91, 352, 610
Eam1104I CTCTTC 1 cut(s) 82
EarI CTCTTC 1 cut(s) 82
EciI GGCGGA 2 cut(s) 89, 137
Ecl136II GAGCTC 1 cut(s) 62
Eco130I CCWWGG 1 cut(s) 627
Eco24I GRGCYC 1 cut(s) 64
Eco47I GGWCC 3 cut(s) 109, 397, 704
Eco53kI GAGCTC 1 cut(s) 62
Eco57I CTGAAG 1 cut(s) 538
Eco88I CYCGRG 1 cut(s) 95
Eco91I GGTNACC 1 cut(s) 167
EcoICRI GAGCTC 1 cut(s) 62
EcoO109I RGGNCCY 1 cut(s) 109
EcoO65I GGTNACC 1 cut(s) 167
EcoT14I CCWWGG 1 cut(s) 627
EcoT38I GRGCYC 1 cut(s) 64
ErhI CCWWGG 1 cut(s) 627
Esp3I CGTCTC 1 cut(s) 142
FaeI CATG 4 cut(s) 320, 473, 631, 637
FaiI YATR 7 cut(s) 236, 318, 471, 534, 586, 629, 635
FaqI GGGAC 1 cut(s) 281
FatI CATG 4 cut(s) 316, 469, 627, 633
FauI CCCGC 1 cut(s) 93
FblI GTMKAC 1 cut(s) 539
Fnu4HI GCNGC 2 cut(s) 6, 72
FokI GGATG 3 cut(s) 338, 366, 535
FriOI GRGCYC 1 cut(s) 64
Fsp4HI GCNGC 2 cut(s) 6, 72
FspBI CTAG 4 cut(s) 350, 447, 461, 653
GluI GCNGC 2 cut(s) 6, 72
GsuI CTGGAG 1 cut(s) 312
HapII CCGG 2 cut(s) 227, 504
HgaI GACGC 1 cut(s) 170
Hin1II CATG 4 cut(s) 320, 473, 631, 637
HinfI GANTC 3 cut(s) 52, 294, 435
HpaII CCGG 2 cut(s) 227, 504
HphI GGTGA 2 cut(s) 161, 653
Hpy166II GTNNAC 2 cut(s) 406, 540
Hpy188I TCNGA 1 cut(s) 704
Hpy188III TCNNGA 7 cut(s) 95, 197, 227, 350, 432, 504, 556
Hpy8I GTNNAC 2 cut(s) 406, 540
Hpy99I CGWCG 2 cut(s) 32, 164
HpyAV CCTTC 2 cut(s) 122, 453
HpyCH4III ACNGT 5 cut(s) 28, 173, 485, 572, 624
HpyCH4IV ACGT 2 cut(s) 192, 536
HpyCH4V TGCA 1 cut(s) 588
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 20, 29, 68
HpyF3I CTNAG 3 cut(s) 56, 606, 732
HpySE526I ACGT 2 cut(s) 192, 536
Hsp92II CATG 4 cut(s) 320, 473, 631, 637
Kpn2I TCCGGA 2 cut(s) 226, 503
Kzo9I GATC 3 cut(s) 91, 352, 610
LmnI GCTCC 3 cut(s) 13, 76, 220
Lsp1109I GCAGC 1 cut(s) 17
LweI GCATC 1 cut(s) 513
MaeI CTAG 4 cut(s) 350, 447, 461, 653
MaeII ACGT 2 cut(s) 192, 536
MaeIII GTNAC 2 cut(s) 167, 641
MalI GATC 3 cut(s) 93, 354, 612
MbiI CCGCTC 2 cut(s) 45, 100
MboI GATC 3 cut(s) 91, 352, 610
MboII GAAGA 2 cut(s) 99, 242
MflI RGATCY 1 cut(s) 91
MhlI GDGCHC 2 cut(s) 40, 64
MluCI AATT 3 cut(s) 187, 301, 614
MlyI GAGTC 1 cut(s) 303
MnlI CCTC 3 cut(s) 13, 601, 675
MroI TCCGGA 2 cut(s) 226, 503
MseI TTAA 1 cut(s) 509
MslI CAYNNNNRTG 2 cut(s) 632, 677
MspI CCGG 2 cut(s) 227, 504
MwoI GCNNNNNNNGC 4 cut(s) 11, 20, 29, 68
NcoI CCATGG 1 cut(s) 627
NdeII GATC 3 cut(s) 91, 352, 610
NheI GCTAGC 2 cut(s) 460, 652
NlaIII CATG 4 cut(s) 320, 473, 631, 637
NlaIV GGNNCC 2 cut(s) 110, 691
NmuCI GTSAC 2 cut(s) 167, 641
NspI RCATGY 2 cut(s) 473, 637
OliI CACNNNNGTG 1 cut(s) 677
PciI ACATGT 1 cut(s) 469
PfeI GAWTC 2 cut(s) 52, 435
PkrI GCNGC 2 cut(s) 7, 73
PleI GAGTC 1 cut(s) 302
PpsI GAGTC 1 cut(s) 302
PpuMI RGGWCCY 1 cut(s) 109
PscI ACATGT 1 cut(s) 469
PshAI GACNNNNGTC 2 cut(s) 165, 293
PshBI ATTAAT 1 cut(s) 509
Psp124BI GAGCTC 1 cut(s) 64
Psp5II RGGWCCY 1 cut(s) 109
PspEI GGTNACC 1 cut(s) 167
PspN4I GGNNCC 2 cut(s) 110, 691
PspPI GGNCC 3 cut(s) 109, 397, 704
PspPPI RGGWCCY 1 cut(s) 109
PstNI CAGNNNCTG 1 cut(s) 11
PsuI RGATCY 1 cut(s) 91
RsaI GTAC 1 cut(s) 716
RsaNI GTAC 1 cut(s) 715
RseI CAYNNNNRTG 2 cut(s) 632, 677
SacI GAGCTC 1 cut(s) 64
SaqAI TTAA 1 cut(s) 509
SatI GCNGC 2 cut(s) 6, 72
Sau3AI GATC 3 cut(s) 91, 352, 610
Sau96I GGNCC 3 cut(s) 109, 397, 704
SchI GAGTC 1 cut(s) 303
SduI GDGCHC 2 cut(s) 40, 64
SfaNI GCATC 1 cut(s) 513
SfcI CTRYAG 1 cut(s) 571
SinI GGWCC 3 cut(s) 109, 397, 704
SmiMI CAYNNNNRTG 2 cut(s) 632, 677
Sse9I AATT 3 cut(s) 187, 301, 614
SsiI CCGC 7 cut(s) 14, 43, 71, 74, 100, 148, 414
SspMI CTAG 4 cut(s) 350, 447, 461, 653
SstI GAGCTC 1 cut(s) 64
StyI CCWWGG 1 cut(s) 627
TaaI ACNGT 5 cut(s) 28, 173, 485, 572, 624
TaiI ACGT 2 cut(s) 195, 539
TaqI TCGA 4 cut(s) 207, 355, 438, 648
TasI AATT 3 cut(s) 187, 301, 614
TauI GCSGC 1 cut(s) 74
TfiI GAWTC 2 cut(s) 52, 435
Tru1I TTAA 1 cut(s) 509
Tru9I TTAA 1 cut(s) 509
TscAI CASTG 1 cut(s) 488
TseFI GTSAC 2 cut(s) 167, 641
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 2 cut(s) 167, 641
TspDTI ATGAA 1 cut(s) 305
TspRI CASTG 1 cut(s) 488
VpaK11BI GGWCC 3 cut(s) 109, 397, 704
VspI ATTAAT 1 cut(s) 509
XapI RAATTY 1 cut(s) 301
XbaI TCTAGA 1 cut(s) 349
XceI RCATGY 2 cut(s) 473, 637
XmiI GTMKAC 1 cut(s) 539
XspI CTAG 4 cut(s) 350, 447, 461, 653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.