FvH4_3g23210

Pyridoxal 5'-phosphate (PLP)-binding protein, which may be involved in intracellular homeostatic regulation of pyridoxal 5'-phosphate (PLP), the active form of vitamin B6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0017509
Physical Location & Seq
Forward (+)
16286674 .. 16289596
2923 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g23210.t1

Sequence Viewer

Length: 738 bp
ATGGCTGCTGCAGCTGTCGAAAACGTCGCCGTATCGGCGCTCCGGTCCGTCGTTCTCCGAGTCCGTCAGGCTGCCGAACGGTCAGGCCGGAATCCAAGTCAGATCAGACTGGTAGCGGTGTCAAAGACCAAGCCCATTTCTCTCATCCGGCAAGTCTACGACGCCGGCCACCGTCGATTCGGTGAGAATTACGTCCAAGAAATGCTGGAGAAAGCTCCTCAGCTTCCTGAAGATATAGAGTGGCATTTCATTGGGCATTTGCAGAGCAACAAAGTCAAATCGCTTCTGGCTGGAGTGCCGAATCTGGCCTTGGTTGAGGGAGTAGACAATGTGAAGCTAGCAAATCATCTTGATCGGGCAGTTTCCAACCTTGGGAGAAATCCTTTAAAGGTATTGGTCCAAGTTAATACCAGTGGAGAAGAATCTAAATCTGGTGTAGATCCTTCTGGTTGTGTTGAACTTGCAAAACATGTCAAATTGCAGTGCCCAAATCTAGTGCTTTCTGGCTTAATGACAATAGGGATGCGTGACTATACTTCAACTCCAGAAAACTTCAGGATGCTATCAAAGTGTAGAACTGAGGTATGCAAGGAGCTTGGTATGGCAGAGGAGAACTTTGAGCTGTCAATGGGCATGTCTGGTGATTTTGAGCAAGCGATTGAAATGGGCAGCACCAATGTCAGAGTTGGATCCACCATATTTGGGCCAAGGGAGTATCCAAAGAAACAAGCAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ala_racemase_N

Protein Analysis

246

Amino Acids

26.89

Weight (kDa)

8.66

Isoelectric Point (pI)

38.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ala_racemase_N PF01168 22 - 236 1.2e-15 Alanine racemase, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017509)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26860 AT4G26860
fragaria_vesca FvH4_3g23210
malus_domestica MD03G1196600.v1.1
prunus_persica Prupe.4G218800_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0040871
rosa_laevigata RLG00000034010
rosa_roxburghii Rroxscaffold_1G00039830
rosa_rugosa Rorug05G0188200
rosa_samantha Rh5AG273900 Rh5BG278900
rosa_wichuraiana Rw5G025740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 156, 324
AciI CCGC 1 cut(s) 116
AclWI GGATC 3 cut(s) 434, 684, 697
AcoI YGGCCR 1 cut(s) 166
AcuI CTGAAG 2 cut(s) 249, 538
AcyI GRCGYC 1 cut(s) 162
AfiI CCNNNNNNNGG 2 cut(s) 372, 702
AflIII ACRYGT 1 cut(s) 469
AgsI TTSAA 3 cut(s) 458, 540, 662
AjuI GAANNNNNNNTTGG 2 cut(s) 293, 325
AluBI AGCT 6 cut(s) 14, 215, 223, 337, 595, 622
AluI AGCT 6 cut(s) 14, 215, 223, 337, 595, 622
AlwI GGATC 3 cut(s) 434, 684, 697
AoxI GGCC 4 cut(s) 85, 166, 306, 704
ApeKI GCWGC 5 cut(s) 5, 8, 11, 71, 669
AspLEI GCGC 1 cut(s) 40
AspS9I GGNCC 3 cut(s) 45, 397, 704
AsuHPI GGTGA 2 cut(s) 194, 653
AsuNHI GCTAGC 1 cut(s) 337
AvaII GGWCC 2 cut(s) 45, 397
BaeGI GKGCMC 1 cut(s) 488
BamHI GGATCC 1 cut(s) 689
BbvCI CCTCAGC 1 cut(s) 219
BbvI GCAGC 3 cut(s) 23, 58, 681
BceAI ACGGC 1 cut(s) 14
BciVI GTATCC 1 cut(s) 726
BfaI CTAG 2 cut(s) 338, 494
BfmI CTRYAG 1 cut(s) 9
BfoI RGCGCY 1 cut(s) 41
BfuI GTATCC 1 cut(s) 726
BglI GCCNNNNNGGC 1 cut(s) 35
BisI GCNGC 5 cut(s) 6, 9, 12, 72, 670
BlsI GCNGC 5 cut(s) 7, 10, 13, 73, 671
Bme18I GGWCC 2 cut(s) 45, 397
BmgT120I GGNCC 3 cut(s) 45, 397, 704
BmiI GGNNCC 1 cut(s) 691
BmsI GCATC 2 cut(s) 513, 549
BmtI GCTAGC 1 cut(s) 341
BpmI CTGGAG 3 cut(s) 227, 312, 528
Bpu10I CCTNAGC 1 cut(s) 219
BsaHI GRCGYC 1 cut(s) 162
BsaJI CCNNGG 3 cut(s) 309, 370, 707
BsaWI WCCGGW 1 cut(s) 42
BsaXI ACNNNNNCTCC 2 cut(s) 526, 556
Bsc4I CCNNNNNNNGG 2 cut(s) 372, 702
Bse118I RCCGGY 1 cut(s) 164
Bse1I ACTGG 2 cut(s) 114, 411
BseDI CCNNGG 3 cut(s) 309, 370, 707
BseGI GGATG 3 cut(s) 144, 528, 564
BseLI CCNNNNNNNGG 2 cut(s) 372, 702
BseMII CTCAG 2 cut(s) 233, 570
BseNI ACTGG 2 cut(s) 114, 411
BseRI GAGGAG 2 cut(s) 207, 623
BseSI GKGCMC 1 cut(s) 488
BseXI GCAGC 3 cut(s) 23, 58, 681
BshFI GGCC 4 cut(s) 87, 168, 308, 706
BsiSI CCGG 4 cut(s) 43, 88, 148, 165
BslI CCNNNNNNNGG 2 cut(s) 372, 702
BsnI GGCC 4 cut(s) 87, 168, 308, 706
Bsp1286I GDGCHC 1 cut(s) 488
Bsp143I GATC 4 cut(s) 102, 352, 439, 689
BspACI CCGC 1 cut(s) 116
BspANI GGCC 4 cut(s) 87, 168, 308, 706
BspCNI CTCAG 2 cut(s) 232, 571
BspLI GGNNCC 1 cut(s) 691
BspMAI CTGCAG 1 cut(s) 13
BspOI GCTAGC 1 cut(s) 341
BspPI GGATC 3 cut(s) 434, 684, 697
BsrFI RCCGGY 1 cut(s) 164
BsrI ACTGG 2 cut(s) 114, 411
BssAI RCCGGY 1 cut(s) 164
BssECI CCNNGG 3 cut(s) 309, 370, 707
BssMI GATC 4 cut(s) 102, 352, 439, 689
BssNI GRCGYC 1 cut(s) 162
BssT1I CCWWGG 3 cut(s) 309, 370, 707
Bst4CI ACNGT 2 cut(s) 81, 173
BstACI GRCGYC 1 cut(s) 162
BstC8I GCNNGC 3 cut(s) 166, 339, 654
BstDEI CTNAG 2 cut(s) 219, 579
BstF5I GGATG 3 cut(s) 144, 528, 564
BstH2I RGCGCY 1 cut(s) 41
BstHHI GCGC 1 cut(s) 40
BstKTI GATC 4 cut(s) 105, 355, 442, 692
BstMBI GATC 4 cut(s) 102, 352, 439, 689
BstMWI GCNNNNNNNGC 2 cut(s) 11, 35
BstNSI RCATGY 2 cut(s) 473, 637
BstSFI CTRYAG 1 cut(s) 9
BstSLI GKGCMC 1 cut(s) 488
BstV1I GCAGC 3 cut(s) 23, 58, 681
BstX2I RGATCY 2 cut(s) 439, 689
BstYI RGATCY 2 cut(s) 439, 689
BsuI GTATCC 1 cut(s) 726
BsuRI GGCC 4 cut(s) 87, 168, 308, 706
BtsCI GGATG 3 cut(s) 144, 528, 564
BtsI GCAGTG 1 cut(s) 488
BtsIMutI CAGTG 2 cut(s) 418, 488
Cac8I GCNNGC 3 cut(s) 166, 339, 654
CfoI GCGC 1 cut(s) 40
Cfr10I RCCGGY 1 cut(s) 164
Cfr13I GGNCC 3 cut(s) 45, 397, 704
CpoI CGGWCCG 1 cut(s) 45
CseI GACGC 1 cut(s) 170
CspCI CAANNNNNGTGG 2 cut(s) 682, 717
CspI CGGWCCG 1 cut(s) 45
CviAII CATG 2 cut(s) 470, 634
DdeI CTNAG 2 cut(s) 219, 579
DpnI GATC 4 cut(s) 104, 354, 441, 691
DpnII GATC 4 cut(s) 102, 352, 439, 689
DraI TTTAAA 1 cut(s) 387
EaeI YGGCCR 1 cut(s) 166
Eco130I CCWWGG 3 cut(s) 309, 370, 707
Eco47I GGWCC 2 cut(s) 45, 397
Eco57I CTGAAG 2 cut(s) 249, 538
EcoT14I CCWWGG 3 cut(s) 309, 370, 707
ErhI CCWWGG 3 cut(s) 309, 370, 707
FaeI CATG 2 cut(s) 473, 637
FaiI YATR 7 cut(s) 236, 471, 534, 586, 602, 635, 698
FatI CATG 2 cut(s) 469, 633
FblI GTMKAC 2 cut(s) 156, 324
Fnu4HI GCNGC 5 cut(s) 6, 9, 12, 72, 670
FokI GGATG 3 cut(s) 131, 535, 571
Fsp4HI GCNGC 5 cut(s) 6, 9, 12, 72, 670
FspBI CTAG 2 cut(s) 338, 494
GlaI GCGC 1 cut(s) 39
GluI GCNGC 5 cut(s) 6, 9, 12, 72, 670
GsuI CTGGAG 3 cut(s) 227, 312, 528
HaeII RGCGCY 1 cut(s) 41
HaeIII GGCC 4 cut(s) 87, 168, 308, 706
HapII CCGG 4 cut(s) 43, 88, 148, 165
HgaI GACGC 1 cut(s) 170
HhaI GCGC 1 cut(s) 40
Hin1I GRCGYC 1 cut(s) 162
Hin1II CATG 2 cut(s) 473, 637
Hin6I GCGC 1 cut(s) 38
HinP1I GCGC 1 cut(s) 38
HinfI GANTC 5 cut(s) 60, 91, 177, 301, 422
HpaII CCGG 4 cut(s) 43, 88, 148, 165
HphI GGTGA 2 cut(s) 194, 653
Hpy166II GTNNAC 2 cut(s) 157, 325
Hpy188I TCNGA 4 cut(s) 59, 102, 107, 683
Hpy188III TCNNGA 4 cut(s) 227, 350, 545, 556
Hpy8I GTNNAC 2 cut(s) 157, 325
Hpy99I CGWCG 4 cut(s) 29, 53, 164, 177
HpyAV CCTTC 1 cut(s) 453
HpyCH4III ACNGT 2 cut(s) 81, 173
HpyCH4IV ACGT 2 cut(s) 24, 192
HpyCH4V TGCA 5 cut(s) 11, 262, 464, 481, 588
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 35
HpyF3I CTNAG 2 cut(s) 219, 579
HpySE526I ACGT 2 cut(s) 24, 192
Hsp92I GRCGYC 1 cut(s) 162
Hsp92II CATG 2 cut(s) 473, 637
HspAI GCGC 1 cut(s) 38
KroI GCCGGC 1 cut(s) 164
KroNI GCCGGC 1 cut(s) 166
Kzo9I GATC 4 cut(s) 102, 352, 439, 689
LmnI GCTCC 3 cut(s) 45, 220, 592
Lsp1109I GCAGC 3 cut(s) 23, 58, 681
LweI GCATC 2 cut(s) 513, 549
MaeI CTAG 2 cut(s) 338, 494
MaeII ACGT 2 cut(s) 24, 192
MaeIII GTNAC 1 cut(s) 527
MalI GATC 4 cut(s) 104, 354, 441, 691
MboI GATC 4 cut(s) 102, 352, 439, 689
MboII GAAGA 2 cut(s) 242, 431
MflI RGATCY 2 cut(s) 439, 689
MhlI GDGCHC 1 cut(s) 488
MluCI AATT 3 cut(s) 187, 476, 733
MlyI GAGTC 1 cut(s) 69
MmeI TCCRAC 2 cut(s) 390, 667
MnlI CCTC 4 cut(s) 228, 310, 574, 601
MroNI GCCGGC 1 cut(s) 164
MseI TTAA 3 cut(s) 386, 405, 509
MspA1I CMGCKG 1 cut(s) 14
MspI CCGG 4 cut(s) 43, 88, 148, 165
MwoI GCNNNNNNNGC 2 cut(s) 11, 35
NaeI GCCGGC 1 cut(s) 166
NdeII GATC 4 cut(s) 102, 352, 439, 689
NgoMIV GCCGGC 1 cut(s) 164
NheI GCTAGC 1 cut(s) 337
NlaIII CATG 2 cut(s) 473, 637
NlaIV GGNNCC 1 cut(s) 691
NmuCI GTSAC 1 cut(s) 527
NspI RCATGY 2 cut(s) 473, 637
PciI ACATGT 1 cut(s) 469
PdiI GCCGGC 1 cut(s) 166
PfeI GAWTC 4 cut(s) 91, 177, 301, 422
PkrI GCNGC 5 cut(s) 7, 10, 13, 73, 671
PleI GAGTC 1 cut(s) 68
PpsI GAGTC 1 cut(s) 68
PscI ACATGT 1 cut(s) 469
PspN4I GGNNCC 1 cut(s) 691
PspPI GGNCC 3 cut(s) 45, 397, 704
PstI CTGCAG 1 cut(s) 13
PsuI RGATCY 2 cut(s) 439, 689
PvuII CAGCTG 1 cut(s) 14
Rsr2I CGGWCCG 1 cut(s) 45
RsrII CGGWCCG 1 cut(s) 45
SaqAI TTAA 3 cut(s) 386, 405, 509
SatI GCNGC 5 cut(s) 6, 9, 12, 72, 670
Sau3AI GATC 4 cut(s) 102, 352, 439, 689
Sau96I GGNCC 3 cut(s) 45, 397, 704
SchI GAGTC 1 cut(s) 69
SduI GDGCHC 1 cut(s) 488
SfaNI GCATC 2 cut(s) 513, 549
SfcI CTRYAG 1 cut(s) 9
SinI GGWCC 2 cut(s) 45, 397
Sse9I AATT 3 cut(s) 187, 476, 733
SsiI CCGC 1 cut(s) 116
SspMI CTAG 2 cut(s) 338, 494
StyI CCWWGG 3 cut(s) 309, 370, 707
TaaI ACNGT 2 cut(s) 81, 173
TaiI ACGT 2 cut(s) 27, 195
TaqI TCGA 2 cut(s) 18, 175
TasI AATT 3 cut(s) 187, 476, 733
TfiI GAWTC 4 cut(s) 91, 177, 301, 422
Tru1I TTAA 3 cut(s) 386, 405, 509
Tru9I TTAA 3 cut(s) 386, 405, 509
TscAI CASTG 2 cut(s) 418, 488
TseFI GTSAC 1 cut(s) 527
TseI GCWGC 5 cut(s) 5, 8, 11, 71, 669
Tsp45I GTSAC 1 cut(s) 527
TspDTI ATGAA 1 cut(s) 238
TspGWI ACGGA 2 cut(s) 37, 53
TspRI CASTG 2 cut(s) 418, 488
VpaK11BI GGWCC 2 cut(s) 45, 397
XceI RCATGY 2 cut(s) 473, 637
XmiI GTMKAC 2 cut(s) 156, 324
XspI CTAG 2 cut(s) 338, 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.