MD03G1196600.v1.1

Pyridoxal 5'-phosphate (PLP)-binding protein, which may be involved in intracellular homeostatic regulation of pyridoxal 5'-phosphate (PLP), the active form of vitamin B6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0017509
Physical Location & Seq
Reverse (-)
26894863 .. 26898253
3391 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1196600.v1.1.491

Sequence Viewer

Length: 738 bp
ATGGCTGCTTCCGCTGTCGAAAACGCCGGCGTCTCGGCGCTCCGGTCAGTCTTGCTCCGGGTCCGGCAGGCTGCGGAGCGGTCGGGTCGGGATCCGGCTCCGGTGAGGCTGGTAGCGGTGTCGAAGACCAAGCCCGTTTCTCTCATCCGCCAAGTGTACAACTTCGGCCACCGCTGTTTTGGCGAGAATTACGTCCAGGAAATCCTCGAAAAAGCTCCTCAGCTTCCTGATGACATAGAGTGGCATTTCGTTGGGCATTTGCAGACCAACAAGGCGAAGTTGCTTCTGGCTGGAGTCCCGAATTTGGCCTTGGTTGAAGGTGTAGACAATGAGAAGATTGCAAATCATCTTGATCGAGCGGTGTCAAACCTTGGAAGAAATCCTTTAAAGGTTTTGGTCCAAGTAAATACCAGTGGAGAAGAATCGAAATCTGGAATTCATCCTTCTGGTTGTGTTGAACTTGCGAAACACGTCAAGTTTCAGTGCCCAAATCTACAGTTTTCTGGCTTAATGACCATAGGGATGCCGGACTATACATCCACTCCAGAAAACTTCAGGATGCTATCAAAGTGTAGAACGGAGGTCTGCAAGGCACTTGCTATGGCAGAGGAGCATTGTGAGCTGTCAATGGGCATGTCTGGTGACTTTGAGCAAGCGATTGAAATGGGCAGCACCAATGTCAGAATCGGATCCACCATATTTGGCCCTAGGGAGTACCCAAAGAAGCAAACAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ala_racemase_N

Protein Analysis

246

Amino Acids

26.87

Weight (kDa)

7.65

Isoelectric Point (pI)

35.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ala_racemase_N PF01168 22 - 236 2.4e-15 Alanine racemase, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017509)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26860 AT4G26860
fragaria_vesca FvH4_3g23210
malus_domestica MD03G1196600.v1.1
prunus_persica Prupe.4G218800_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0040871
rosa_laevigata RLG00000034010
rosa_roxburghii Rroxscaffold_1G00039830
rosa_rugosa Rorug05G0188200
rosa_samantha Rh5AG273900 Rh5BG278900
rosa_wichuraiana Rw5G025740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 79, 359
AccI GTMKAC 1 cut(s) 324
AciI CCGC 7 cut(s) 12, 74, 79, 116, 148, 172, 359
AclWI GGATC 4 cut(s) 86, 99, 684, 697
AcoI YGGCCR 1 cut(s) 166
AcsI RAATTY 2 cut(s) 301, 435
AcuI CTGAAG 1 cut(s) 538
AcyI GRCGYC 1 cut(s) 30
AfaI GTAC 2 cut(s) 158, 716
AfiI CCNNNNNNNGG 1 cut(s) 304
AflIII ACRYGT 1 cut(s) 469
AgsI TTSAA 3 cut(s) 317, 458, 662
AjiI CACGTC 1 cut(s) 472
AjnI CCWGG 1 cut(s) 195
AjuI GAANNNNNNNTTGG 2 cut(s) 293, 325
AluBI AGCT 3 cut(s) 215, 223, 622
AluI AGCT 3 cut(s) 215, 223, 622
Alw26I GTCTC 1 cut(s) 37
AlwI GGATC 4 cut(s) 86, 99, 684, 697
AoxI GGCC 3 cut(s) 166, 306, 703
ApeKI GCWGC 3 cut(s) 5, 71, 669
ApoI RAATTY 2 cut(s) 301, 435
AspA2I CCTAGG 1 cut(s) 707
AspLEI GCGC 1 cut(s) 40
AspS9I GGNCC 3 cut(s) 61, 397, 704
AsuC2I CCSGG 1 cut(s) 59
AsuHPI GGTGA 2 cut(s) 115, 653
AvaII GGWCC 2 cut(s) 61, 397
AvrII CCTAGG 1 cut(s) 707
BaeGI GKGCMC 1 cut(s) 488
BamHI GGATCC 2 cut(s) 91, 689
BbsI GAAGAC 1 cut(s) 131
BbvCI CCTCAGC 1 cut(s) 219
BbvI GCAGC 2 cut(s) 58, 681
BciT130I CCWGG 1 cut(s) 197
BcnI CCSGG 1 cut(s) 59
BcoDI GTCTC 1 cut(s) 37
BfaI CTAG 1 cut(s) 708
BfmI CTRYAG 1 cut(s) 494
BfoI RGCGCY 1 cut(s) 41
BisI GCNGC 3 cut(s) 6, 72, 670
BlnI CCTAGG 1 cut(s) 707
BlsI GCNGC 3 cut(s) 7, 73, 671
Bme1390I CCNGG 2 cut(s) 59, 197
Bme18I GGWCC 2 cut(s) 61, 397
BmgBI CACGTC 1 cut(s) 472
BmgT120I GGNCC 3 cut(s) 61, 397, 704
BmiI GGNNCC 4 cut(s) 62, 93, 99, 691
BmrFI CCNGG 2 cut(s) 59, 197
BmsI GCATC 2 cut(s) 513, 549
BpiI GAAGAC 1 cut(s) 131
BpmI CTGGAG 2 cut(s) 312, 528
Bpu10I CCTNAGC 1 cut(s) 219
BpuMI CCSGG 1 cut(s) 59
BsaHI GRCGYC 1 cut(s) 30
BsaJI CCNNGG 3 cut(s) 309, 370, 707
BsaWI WCCGGW 2 cut(s) 42, 100
BsaXI ACNNNNNCTCC 2 cut(s) 526, 556
Bsc4I CCNNNNNNNGG 1 cut(s) 304
Bse118I RCCGGY 1 cut(s) 26
Bse1I ACTGG 1 cut(s) 411
BseBI CCWGG 1 cut(s) 197
BseDI CCNNGG 3 cut(s) 309, 370, 707
BseGI GGATG 5 cut(s) 144, 439, 528, 536, 564
BseLI CCNNNNNNNGG 1 cut(s) 304
BseMII CTCAG 1 cut(s) 233
BseNI ACTGG 1 cut(s) 411
BseRI GAGGAG 2 cut(s) 207, 623
BseSI GKGCMC 1 cut(s) 488
BseXI GCAGC 2 cut(s) 58, 681
Bsh1285I CGRYCG 1 cut(s) 83
BshFI GGCC 3 cut(s) 168, 308, 705
BsiEI CGRYCG 1 cut(s) 83
BsiSI CCGG 7 cut(s) 27, 43, 58, 64, 95, 101, 527
BslFI GGGAC 1 cut(s) 281
BslI CCNNNNNNNGG 1 cut(s) 304
BsmAI GTCTC 1 cut(s) 37
BsmBI CGTCTC 1 cut(s) 37
BsmFI GGGAC 1 cut(s) 281
BsnI GGCC 3 cut(s) 168, 308, 705
Bsp1286I GDGCHC 1 cut(s) 488
Bsp1407I TGTACA 1 cut(s) 156
Bsp143I GATC 3 cut(s) 91, 352, 689
BspACI CCGC 7 cut(s) 12, 74, 79, 116, 148, 172, 359
BspANI GGCC 3 cut(s) 168, 308, 705
BspCNI CTCAG 1 cut(s) 232
BspLI GGNNCC 4 cut(s) 62, 93, 99, 691
BspPI GGATC 4 cut(s) 86, 99, 684, 697
BsrBI CCGCTC 2 cut(s) 79, 359
BsrFI RCCGGY 1 cut(s) 26
BsrGI TGTACA 1 cut(s) 156
BsrI ACTGG 1 cut(s) 411
BssAI RCCGGY 1 cut(s) 26
BssECI CCNNGG 3 cut(s) 309, 370, 707
BssMI GATC 3 cut(s) 91, 352, 689
BssNI GRCGYC 1 cut(s) 30
BssT1I CCWWGG 3 cut(s) 309, 370, 707
Bst2UI CCWGG 1 cut(s) 197
Bst4CI ACNGT 1 cut(s) 498
BstACI GRCGYC 1 cut(s) 30
BstAUI TGTACA 1 cut(s) 156
BstC8I GCNNGC 3 cut(s) 28, 69, 654
BstDEI CTNAG 1 cut(s) 219
BstF5I GGATG 5 cut(s) 144, 439, 528, 536, 564
BstH2I RGCGCY 1 cut(s) 41
BstHHI GCGC 1 cut(s) 40
BstKTI GATC 3 cut(s) 94, 355, 692
BstMAI GTCTC 1 cut(s) 37
BstMBI GATC 3 cut(s) 91, 352, 689
BstMCI CGRYCG 1 cut(s) 83
BstMWI GCNNNNNNNGC 3 cut(s) 11, 180, 619
BstNI CCWGG 1 cut(s) 197
BstNSI RCATGY 1 cut(s) 637
BstSCI CCNGG 2 cut(s) 57, 195
BstSFI CTRYAG 1 cut(s) 494
BstSLI GKGCMC 1 cut(s) 488
BstV1I GCAGC 2 cut(s) 58, 681
BstV2I GAAGAC 1 cut(s) 131
BstX2I RGATCY 2 cut(s) 91, 689
BstYI RGATCY 2 cut(s) 91, 689
BsuRI GGCC 3 cut(s) 168, 308, 705
BtrI CACGTC 1 cut(s) 472
BtsCI GGATG 5 cut(s) 144, 439, 528, 536, 564
BtsIMutI CAGTG 2 cut(s) 418, 488
Cac8I GCNNGC 3 cut(s) 28, 69, 654
CfoI GCGC 1 cut(s) 40
Cfr10I RCCGGY 1 cut(s) 26
Cfr13I GGNCC 3 cut(s) 61, 397, 704
CseI GACGC 1 cut(s) 19
Csp6I GTAC 2 cut(s) 157, 715
CspCI CAANNNNNGTGG 2 cut(s) 682, 717
CviAII CATG 1 cut(s) 634
CviQI GTAC 2 cut(s) 157, 715
DdeI CTNAG 1 cut(s) 219
DpnI GATC 3 cut(s) 93, 354, 691
DpnII GATC 3 cut(s) 91, 352, 689
DraI TTTAAA 1 cut(s) 387
EaeI YGGCCR 1 cut(s) 166
EciI GGCGGA 1 cut(s) 137
Eco130I CCWWGG 3 cut(s) 309, 370, 707
Eco47I GGWCC 2 cut(s) 61, 397
Eco57I CTGAAG 1 cut(s) 538
EcoRI GAATTC 1 cut(s) 435
EcoRII CCWGG 1 cut(s) 195
EcoT14I CCWWGG 3 cut(s) 309, 370, 707
ErhI CCWWGG 3 cut(s) 309, 370, 707
Esp3I CGTCTC 1 cut(s) 37
FaeI CATG 1 cut(s) 637
FaiI YATR 6 cut(s) 236, 518, 534, 602, 635, 698
FalI AAGNNNNNCTT 2 cut(s) 367, 399
FaqI GGGAC 1 cut(s) 281
FatI CATG 1 cut(s) 633
FblI GTMKAC 1 cut(s) 324
Fnu4HI GCNGC 3 cut(s) 6, 72, 670
FokI GGATG 5 cut(s) 131, 426, 523, 535, 571
Fsp4HI GCNGC 3 cut(s) 6, 72, 670
FspBI CTAG 1 cut(s) 708
GlaI GCGC 1 cut(s) 39
GluI GCNGC 3 cut(s) 6, 72, 670
GsuI CTGGAG 2 cut(s) 312, 528
HaeII RGCGCY 1 cut(s) 41
HaeIII GGCC 3 cut(s) 168, 308, 705
HapII CCGG 7 cut(s) 27, 43, 58, 64, 95, 101, 527
HgaI GACGC 1 cut(s) 19
HhaI GCGC 1 cut(s) 40
Hin1I GRCGYC 1 cut(s) 30
Hin1II CATG 1 cut(s) 637
Hin6I GCGC 1 cut(s) 38
HinP1I GCGC 1 cut(s) 38
HinfI GANTC 3 cut(s) 294, 422, 684
HpaII CCGG 7 cut(s) 27, 43, 58, 64, 95, 101, 527
HphI GGTGA 2 cut(s) 115, 653
Hpy166II GTNNAC 2 cut(s) 157, 325
Hpy188I TCNGA 2 cut(s) 683, 689
Hpy188III TCNNGA 7 cut(s) 89, 227, 298, 350, 432, 545, 556
Hpy8I GTNNAC 2 cut(s) 157, 325
HpyAV CCTTC 2 cut(s) 311, 453
HpyCH4III ACNGT 1 cut(s) 498
HpyCH4IV ACGT 2 cut(s) 192, 471
HpyCH4V TGCA 3 cut(s) 262, 341, 588
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 180, 619
HpyF3I CTNAG 1 cut(s) 219
HpySE526I ACGT 2 cut(s) 192, 471
Hsp92I GRCGYC 1 cut(s) 30
Hsp92II CATG 1 cut(s) 637
HspAI GCGC 1 cut(s) 38
KroI GCCGGC 1 cut(s) 26
KroNI GCCGGC 1 cut(s) 28
Kzo9I GATC 3 cut(s) 91, 352, 689
LmnI GCTCC 6 cut(s) 45, 60, 76, 103, 220, 610
Lsp1109I GCAGC 2 cut(s) 58, 681
LweI GCATC 2 cut(s) 513, 549
MaeI CTAG 1 cut(s) 708
MaeII ACGT 2 cut(s) 192, 471
MaeIII GTNAC 1 cut(s) 641
MalI GATC 3 cut(s) 93, 354, 691
MbiI CCGCTC 2 cut(s) 79, 359
MboI GATC 3 cut(s) 91, 352, 689
MboII GAAGA 4 cut(s) 136, 346, 387, 431
MflI RGATCY 2 cut(s) 91, 689
MhlI GDGCHC 1 cut(s) 488
MluCI AATT 3 cut(s) 187, 301, 435
MlyI GAGTC 1 cut(s) 303
MnlI CCTC 5 cut(s) 99, 215, 228, 574, 601
MreI CGCCGGCG 1 cut(s) 26
MroNI GCCGGC 1 cut(s) 26
MseI TTAA 2 cut(s) 386, 509
MslI CAYNNNNRTG 1 cut(s) 521
MspA1I CMGCKG 2 cut(s) 14, 174
MspI CCGG 7 cut(s) 27, 43, 58, 64, 95, 101, 527
MspR9I CCNGG 2 cut(s) 59, 197
MvaI CCWGG 1 cut(s) 197
MwoI GCNNNNNNNGC 3 cut(s) 11, 180, 619
NaeI GCCGGC 1 cut(s) 28
NciI CCSGG 1 cut(s) 59
NdeII GATC 3 cut(s) 91, 352, 689
NgoMIV GCCGGC 1 cut(s) 26
NlaIII CATG 1 cut(s) 637
NlaIV GGNNCC 4 cut(s) 62, 93, 99, 691
NmeAIII GCCGAG 1 cut(s) 14
NmuCI GTSAC 1 cut(s) 641
NspI RCATGY 1 cut(s) 637
PdiI GCCGGC 1 cut(s) 28
PfeI GAWTC 2 cut(s) 422, 684
PfoI TCCNGGA 1 cut(s) 195
PkrI GCNGC 3 cut(s) 7, 73, 671
PleI GAGTC 1 cut(s) 302
PpsI GAGTC 1 cut(s) 302
Psp6I CCWGG 1 cut(s) 195
PspGI CCWGG 1 cut(s) 195
PspN4I GGNNCC 4 cut(s) 62, 93, 99, 691
PspPI GGNCC 3 cut(s) 61, 397, 704
PsuI RGATCY 2 cut(s) 91, 689
RsaI GTAC 2 cut(s) 158, 716
RsaNI GTAC 2 cut(s) 157, 715
RseI CAYNNNNRTG 1 cut(s) 521
SaqAI TTAA 2 cut(s) 386, 509
SatI GCNGC 3 cut(s) 6, 72, 670
Sau3AI GATC 3 cut(s) 91, 352, 689
Sau96I GGNCC 3 cut(s) 61, 397, 704
SchI GAGTC 1 cut(s) 303
ScrFI CCNGG 2 cut(s) 59, 197
SduI GDGCHC 1 cut(s) 488
SetI ASST 9 cut(s) 195, 217, 225, 322, 372, 393, 474, 585, 624
SfaNI GCATC 2 cut(s) 513, 549
SfcI CTRYAG 1 cut(s) 494
SgrAI CRCCGGYG 1 cut(s) 26
SinI GGWCC 2 cut(s) 61, 397
SmiMI CAYNNNNRTG 1 cut(s) 521
Sse9I AATT 3 cut(s) 187, 301, 435
SsiI CCGC 7 cut(s) 12, 74, 79, 116, 148, 172, 359
SspMI CTAG 1 cut(s) 708
StyD4I CCNGG 2 cut(s) 57, 195
StyI CCWWGG 3 cut(s) 309, 370, 707
TaaI ACNGT 1 cut(s) 498
TaiI ACGT 2 cut(s) 195, 474
TaqI TCGA 5 cut(s) 18, 122, 207, 355, 425
TasI AATT 3 cut(s) 187, 301, 435
TatI WGTACW 1 cut(s) 156
TfiI GAWTC 2 cut(s) 422, 684
Tru1I TTAA 2 cut(s) 386, 509
Tru9I TTAA 2 cut(s) 386, 509
TscAI CASTG 2 cut(s) 418, 488
TseFI GTSAC 1 cut(s) 641
TseI GCWGC 3 cut(s) 5, 71, 669
Tsp45I GTSAC 1 cut(s) 641
TspDTI ATGAA 1 cut(s) 428
TspGWI ACGGA 1 cut(s) 593
TspRI CASTG 2 cut(s) 418, 488
VpaK11BI GGWCC 2 cut(s) 61, 397
XapI RAATTY 2 cut(s) 301, 435
XceI RCATGY 1 cut(s) 637
XcmI CCANNNNNNNNNTGG 1 cut(s) 176
XmaJI CCTAGG 1 cut(s) 707
XmiI GTMKAC 1 cut(s) 324
XspI CTAG 1 cut(s) 708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.