AT4G27450

Stem-specific protein TSJT1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0014459
Physical Location & Seq
Reverse (-)
13727370 .. 13729002
1633 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G27450.1

Sequence Viewer

Length: 753 bp
ATGTTGGCTATCTTCCACGAGGCGTTTGCTCATCCGCCGGAGGAACTCAACAGTCCGGCATCTGAGAAATGCTCTAAACAACCAAAACTTCCAGAAGAAACCCTAAATGATTTCTTATTACGTTACCCTCTCAACACTTTCTCCATGTCTTTCGGACAAGCTGCTGTTCTTGCTTACGTTCGTCCCTCTGCTTCCTTCTCCATCCACCAGAGGTTGTTTTGTGGATTTGATGACATCTACTGTCTCTTCTTTGGGAGCTTGAACAATCTGTGTCAGCTAAACAAACAGTATGGTCTCACCAAGACAACAAACGAGGCCATGTTTGTGATCGAAGCTTATAGGACTCTTAGAGACAGAGGTCCTTATCCAGCTGATCAGGTCGTCAAAGACTTAGACGGTAGCTTTTCCTTTGTGGTTTATGATAGCAAAGCCGGCTCTGTCTTCACTGCTCTGGGATCTGATGGAGGGGTGAAGCTATACTGGGGCATAGCTGCTGATGGATCTGTTGTTATATCAGATGATTTGGATGTTATCAAAGAGGGTTGTGCTAAATCTTTTGCTCCATTTCCTACAGGGTGTATGTTCCATAGTGAAGGAGGGTTAATGAGCTTTGAGCATCCAATGAACAAGATCAAGGCGATGCCGAGGGTAGACAGTGAAGGAGTTCTATGCGGTGCCAACTTCAAGGTGGATGTGTACAATCGTGTTAACAGTATCCCTCGTCGAGGAAGTGAAGCCAATTGGTCTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3700

Protein Analysis

250

Amino Acids

27.62

Weight (kDa)

5.56

Isoelectric Point (pI)

34.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3700 PF12481 2 - 230 2.2e-107 Aluminium induced protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014459)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27450
fragaria_vesca FvH4_3g20560
malus_domestica MD03G1224100.v1.1 MD11G1241900.v1.1
prunus_persica Prupe.4G185400_v2.0.a1
pyrus_communis pycom03g17250 pycom11g21360
rosa_chinensis RchiOBHm_Chr5g0034571
rosa_laevigata RLG00000033555
rosa_multiflora Rmu_sc0003126.1_g000004
rosa_roxburghii Rroxscaffold_1G00045760
rosa_rugosa Rorug05G0146500
rosa_samantha Rh5AG238900 Rh5BG239400 Rh5CG269900
rosa_wichuraiana Rw5G021780 Rw5G021870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 674
AccI GTMKAC 1 cut(s) 651
AciI CCGC 2 cut(s) 35, 672
AclWI GGATC 2 cut(s) 463, 508
AfaI GTAC 1 cut(s) 698
AfiI CCNNNNNNNGG 1 cut(s) 725
AgsI TTSAA 2 cut(s) 262, 685
AluBI AGCT 9 cut(s) 161, 258, 277, 335, 371, 402, 475, 491, 609
AluI AGCT 9 cut(s) 161, 258, 277, 335, 371, 402, 475, 491, 609
Alw26I GTCTC 3 cut(s) 248, 299, 345
AlwI GGATC 2 cut(s) 463, 508
AoxI GGCC 1 cut(s) 315
ApeKI GCWGC 2 cut(s) 161, 491
Asp700I GAANNNNTTC 1 cut(s) 663
AspS9I GGNCC 1 cut(s) 359
AsuHPI GGTGA 2 cut(s) 289, 481
AvaII GGWCC 1 cut(s) 359
BanI GGYRCC 1 cut(s) 674
BauI CACGAG 1 cut(s) 17
BbsI GAAGAC 1 cut(s) 433
BbvI GCAGC 2 cut(s) 148, 478
BccI CCATC 3 cut(s) 209, 455, 491
BcgI CGANNNNNNTGC 2 cut(s) 8, 42
BciVI GTATCC 1 cut(s) 725
BclI TGATCA 1 cut(s) 373
BcoDI GTCTC 3 cut(s) 248, 299, 345
BfmI CTRYAG 1 cut(s) 570
BfuI GTATCC 1 cut(s) 725
BisI GCNGC 2 cut(s) 162, 492
BlsI GCNGC 2 cut(s) 163, 493
Bme18I GGWCC 1 cut(s) 359
BmgT120I GGNCC 1 cut(s) 359
BmiI GGNNCC 1 cut(s) 676
BmrI ACTGGG 1 cut(s) 490
BmsI GCATC 3 cut(s) 68, 625, 630
BmuI ACTGGG 1 cut(s) 490
BoxI GACNNNNGTC 1 cut(s) 357
BpiI GAAGAC 1 cut(s) 433
BplI GAGNNNNNCTC 2 cut(s) 56, 88
BsaI GGTCTC 1 cut(s) 299
BsaJI CCNNGG 1 cut(s) 644
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bsc4I CCNNNNNNNGG 1 cut(s) 725
Bse118I RCCGGY 1 cut(s) 431
Bse1I ACTGG 1 cut(s) 485
BseDI CCNNGG 1 cut(s) 644
BseGI GGATG 5 cut(s) 31, 201, 532, 616, 697
BseLI CCNNNNNNNGG 1 cut(s) 725
BseMII CTCAG 1 cut(s) 54
BseNI ACTGG 1 cut(s) 485
BseXI GCAGC 2 cut(s) 148, 478
BshFI GGCC 1 cut(s) 317
BshNI GGYRCC 1 cut(s) 674
BsiSI CCGG 3 cut(s) 38, 56, 432
BslFI GGGAC 1 cut(s) 168
BslI CCNNNNNNNGG 1 cut(s) 725
BsmAI GTCTC 3 cut(s) 248, 299, 345
BsmFI GGGAC 1 cut(s) 168
BsnI GGCC 1 cut(s) 317
Bso31I GGTCTC 1 cut(s) 299
Bsp1407I TGTACA 1 cut(s) 696
Bsp143I GATC 5 cut(s) 327, 373, 455, 500, 630
BspACI CCGC 2 cut(s) 35, 672
BspANI GGCC 1 cut(s) 317
BspCNI CTCAG 1 cut(s) 55
BspLI GGNNCC 1 cut(s) 676
BspPI GGATC 2 cut(s) 463, 508
BspT107I GGYRCC 1 cut(s) 674
BspTNI GGTCTC 1 cut(s) 299
BsrFI RCCGGY 1 cut(s) 431
BsrGI TGTACA 1 cut(s) 696
BsrI ACTGG 1 cut(s) 485
BssAI RCCGGY 1 cut(s) 431
BssECI CCNNGG 1 cut(s) 644
BssMI GATC 5 cut(s) 327, 373, 455, 500, 630
BssSI CACGAG 1 cut(s) 17
Bst2BI CACGAG 1 cut(s) 17
Bst4CI ACNGT 6 cut(s) 53, 242, 288, 398, 656, 713
Bst6I CTCTTC 1 cut(s) 251
BstAUI TGTACA 1 cut(s) 696
BstC8I GCNNGC 1 cut(s) 433
BstDEI CTNAG 3 cut(s) 63, 347, 391
BstENI CCTNNNNNAGG 1 cut(s) 723
BstF5I GGATG 5 cut(s) 31, 201, 532, 616, 697
BstKTI GATC 5 cut(s) 330, 376, 458, 503, 633
BstMAI GTCTC 3 cut(s) 248, 299, 345
BstMBI GATC 5 cut(s) 327, 373, 455, 500, 630
BstMWI GCNNNNNNNGC 2 cut(s) 170, 432
BstPAI GACNNNNGTC 1 cut(s) 357
BstSFI CTRYAG 1 cut(s) 570
BstV1I GCAGC 2 cut(s) 148, 478
BstV2I GAAGAC 1 cut(s) 433
BstX2I RGATCY 2 cut(s) 455, 500
BstYI RGATCY 2 cut(s) 455, 500
BsuI GTATCC 1 cut(s) 725
BsuRI GGCC 1 cut(s) 317
BtgZI GCGATG 1 cut(s) 653
BtsCI GGATG 5 cut(s) 31, 201, 532, 616, 697
BtsI GCAGTG 1 cut(s) 444
BtsIMutI CAGTG 2 cut(s) 444, 661
Cac8I GCNNGC 1 cut(s) 433
Cfr10I RCCGGY 1 cut(s) 431
Cfr13I GGNCC 1 cut(s) 359
Csp6I GTAC 1 cut(s) 697
CviAII CATG 2 cut(s) 145, 319
CviQI GTAC 1 cut(s) 697
DdeI CTNAG 3 cut(s) 63, 347, 391
DpnI GATC 5 cut(s) 329, 375, 457, 502, 632
DpnII GATC 5 cut(s) 327, 373, 455, 500, 630
Eam1104I CTCTTC 1 cut(s) 251
EarI CTCTTC 1 cut(s) 251
EciI GGCGGA 1 cut(s) 24
Eco31I GGTCTC 1 cut(s) 299
Eco47I GGWCC 1 cut(s) 359
EcoNI CCTNNNNNAGG 1 cut(s) 723
EcoO109I RGGNCCY 1 cut(s) 359
FaeI CATG 2 cut(s) 148, 322
FaqI GGGAC 1 cut(s) 168
FatI CATG 2 cut(s) 144, 318
FbaI TGATCA 1 cut(s) 373
FblI GTMKAC 1 cut(s) 651
Fnu4HI GCNGC 2 cut(s) 162, 492
FokI GGATG 5 cut(s) 18, 188, 539, 603, 704
Fsp4HI GCNGC 2 cut(s) 162, 492
GluI GCNGC 2 cut(s) 162, 492
HaeIII GGCC 1 cut(s) 317
HapII CCGG 3 cut(s) 38, 56, 432
Hin1II CATG 2 cut(s) 148, 322
HincII GTYRAC 1 cut(s) 709
HindII GTYRAC 1 cut(s) 709
HindIII AAGCTT 1 cut(s) 333
HinfI GANTC 1 cut(s) 343
HpaI GTTAAC 1 cut(s) 709
HpaII CCGG 3 cut(s) 38, 56, 432
HphI GGTGA 2 cut(s) 289, 481
Hpy166II GTNNAC 3 cut(s) 652, 697, 709
Hpy188I TCNGA 5 cut(s) 64, 155, 460, 517, 752
Hpy188III TCNNGA 1 cut(s) 92
Hpy8I GTNNAC 3 cut(s) 652, 697, 709
Hpy99I CGWCG 1 cut(s) 726
HpyAV CCTTC 3 cut(s) 205, 587, 653
HpyCH4III ACNGT 6 cut(s) 53, 242, 288, 398, 656, 713
HpyCH4IV ACGT 2 cut(s) 121, 177
HpyF10VI GCNNNNNNNGC 2 cut(s) 170, 432
HpyF3I CTNAG 3 cut(s) 63, 347, 391
HpySE526I ACGT 2 cut(s) 121, 177
Hsp92II CATG 2 cut(s) 148, 322
KroI GCCGGC 1 cut(s) 431
KroNI GCCGGC 1 cut(s) 433
Ksp22I TGATCA 1 cut(s) 373
KspAI GTTAAC 1 cut(s) 709
Kzo9I GATC 5 cut(s) 327, 373, 455, 500, 630
LmnI GCTCC 2 cut(s) 255, 565
Lsp1109I GCAGC 2 cut(s) 148, 478
LweI GCATC 3 cut(s) 68, 625, 630
MaeII ACGT 2 cut(s) 121, 177
MaeIII GTNAC 1 cut(s) 122
MalI GATC 5 cut(s) 329, 375, 457, 502, 632
MboI GATC 5 cut(s) 327, 373, 455, 500, 630
MboII GAAGA 4 cut(s) 4, 107, 238, 433
MfeI CAATTG 1 cut(s) 739
MflI RGATCY 2 cut(s) 455, 500
MluCI AATT 1 cut(s) 739
MlyI GAGTC 1 cut(s) 337
MroNI GCCGGC 1 cut(s) 431
MroXI GAANNNNTTC 1 cut(s) 663
MseI TTAA 2 cut(s) 602, 708
MslI CAYNNNNRTG 1 cut(s) 323
MspA1I CMGCKG 1 cut(s) 371
MspI CCGG 3 cut(s) 38, 56, 432
MunI CAATTG 1 cut(s) 739
MwoI GCNNNNNNNGC 2 cut(s) 170, 432
NaeI GCCGGC 1 cut(s) 433
NdeII GATC 5 cut(s) 327, 373, 455, 500, 630
NgoMIV GCCGGC 1 cut(s) 431
NlaIII CATG 2 cut(s) 148, 322
NlaIV GGNNCC 1 cut(s) 676
NmeAIII GCCGAG 1 cut(s) 669
PdiI GCCGGC 1 cut(s) 433
PdmI GAANNNNTTC 1 cut(s) 663
PkrI GCNGC 2 cut(s) 163, 493
PleI GAGTC 1 cut(s) 337
PpsI GAGTC 1 cut(s) 337
PpuMI RGGWCCY 1 cut(s) 359
PshAI GACNNNNGTC 1 cut(s) 357
Psp5II RGGWCCY 1 cut(s) 359
PspN4I GGNNCC 1 cut(s) 676
PspPI GGNCC 1 cut(s) 359
PspPPI RGGWCCY 1 cut(s) 359
PsuI RGATCY 2 cut(s) 455, 500
PvuII CAGCTG 1 cut(s) 371
RsaI GTAC 1 cut(s) 698
RsaNI GTAC 1 cut(s) 697
RseI CAYNNNNRTG 1 cut(s) 323
SaqAI TTAA 2 cut(s) 602, 708
SatI GCNGC 2 cut(s) 162, 492
Sau3AI GATC 5 cut(s) 327, 373, 455, 500, 630
Sau96I GGNCC 1 cut(s) 359
SchI GAGTC 1 cut(s) 337
SfaNI GCATC 3 cut(s) 68, 625, 630
SfcI CTRYAG 1 cut(s) 570
SinI GGWCC 1 cut(s) 359
SmiMI CAYNNNNRTG 1 cut(s) 323
Sse9I AATT 1 cut(s) 739
SsiI CCGC 2 cut(s) 35, 672
TaaI ACNGT 6 cut(s) 53, 242, 288, 398, 656, 713
TaiI ACGT 2 cut(s) 124, 180
TaqI TCGA 2 cut(s) 330, 724
TasI AATT 1 cut(s) 739
TatI WGTACW 1 cut(s) 696
Tru1I TTAA 2 cut(s) 602, 708
Tru9I TTAA 2 cut(s) 602, 708
TscAI CASTG 2 cut(s) 451, 661
TseI GCWGC 2 cut(s) 161, 491
TspDTI ATGAA 1 cut(s) 638
TspRI CASTG 2 cut(s) 451, 661
VpaK11BI GGWCC 1 cut(s) 359
XagI CCTNNNNNAGG 1 cut(s) 723
XcmI CCANNNNNNNNNTGG 1 cut(s) 685
XmiI GTMKAC 1 cut(s) 651
XmnI GAANNNNTTC 1 cut(s) 663
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.