MD03G1224100.v1.1

Stem-specific protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0014459
Physical Location & Seq
Reverse (-)
30922862 .. 30924625
1764 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1224100.v1.1.491

Sequence Viewer

Length: 768 bp
ATGTTGGCCATTTTCCATAAGGCTTTTGCTCATCCACCGGAGGAGCTAAACAGTCCTGCCTCTTACAACGGAAGCAAGAAGCCTAAGCACCCGGAAGAGACTCTCACAGACTTCCTCTCCCACCATCCTCACAACTCCTTCTCCATGGGATTTGGCCATGCTGCTGTCCTTGCTTACGTCAAGCCAGACCAAGGCTCTTACTCAAAACACCCGAGGTTGTTTTGTGGGTTTGATGACATATACTGCTTGTTTTTGGGGAGCTTGAACAATCTGTGTTCACTGAATAAGCAGTATGGTCTAACAAAATGCACGAATGAGGCCATGTTTGTGATTGAAGCTTATAGAACCCTTCGAGATCGCGGTCCGTACCCAGCTGATCAGGTTGTCAAAGATCTTGATGGAAGCTTTGCCTTTGTTGTCTATGACAGCAAAGGTGGCAATGTCTTTGCCGCACTGGGTTCTGATGGTGGAGAGCAGCTCTATTGGGGTATTGCAGCGGATGGATCTGTGGTAATTTCTGATGAATTGGAGGTCATAAAAGAGGGTTGTGCTAAATCATTTGCTCCCTTCCCAAAAGGATGCATGTTTCATAGTGAGGGAGGATTGATGAGCTTTGAGCATCCAATGAACAAGATGAAGGCAATGCCAAGGGTAGATAGTGAAGGGGCAATGTGTGGGGCTAACTTCAAAGTGGATAAATACACAAGGGTCAACAGCATACCACGTGTCGGAAGTGAGGCCAACTGGACTGATTGGGCCTCCCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3700

Protein Analysis

256

Amino Acids

28.08

Weight (kDa)

6.11

Isoelectric Point (pI)

26.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3700 PF12481 2 - 231 4e-108 Aluminium induced protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014459)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27450
fragaria_vesca FvH4_3g20560
malus_domestica MD03G1224100.v1.1 MD11G1241900.v1.1
prunus_persica Prupe.4G185400_v2.0.a1
pyrus_communis pycom03g17250 pycom11g21360
rosa_chinensis RchiOBHm_Chr5g0034571
rosa_laevigata RLG00000033555
rosa_multiflora Rmu_sc0003126.1_g000004
rosa_roxburghii Rroxscaffold_1G00045760
rosa_rugosa Rorug05G0146500
rosa_samantha Rh5AG238900 Rh5BG239400 Rh5CG269900
rosa_wichuraiana Rw5G021780 Rw5G021870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 360
AciI CCGC 3 cut(s) 360, 450, 497
AclWI GGATC 1 cut(s) 511
AcoI YGGCCR 2 cut(s) 6, 154
AcvI CACGTG 1 cut(s) 725
AfaI GTAC 1 cut(s) 368
AfiI CCNNNNNNNGG 2 cut(s) 191, 728
AflIII ACRYGT 1 cut(s) 724
AgsI TTSAA 3 cut(s) 265, 335, 688
AluBI AGCT 7 cut(s) 46, 261, 338, 374, 405, 478, 612
AluI AGCT 7 cut(s) 46, 261, 338, 374, 405, 478, 612
Alw26I GTCTC 1 cut(s) 92
AlwI GGATC 1 cut(s) 511
Ama87I CYCGRG 1 cut(s) 211
AoxI GGCC 5 cut(s) 6, 154, 318, 738, 756
ApeKI GCWGC 3 cut(s) 161, 475, 494
AspS9I GGNCC 2 cut(s) 362, 756
AsuC2I CCSGG 1 cut(s) 92
AvaI CYCGRG 1 cut(s) 211
AvaII GGWCC 1 cut(s) 362
BalI TGGCCA 2 cut(s) 8, 156
BbrPI CACGTG 1 cut(s) 725
BbvI GCAGC 3 cut(s) 148, 487, 506
BccI CCATC 4 cut(s) 132, 392, 458, 494
BclI TGATCA 1 cut(s) 376
BcnI CCSGG 1 cut(s) 92
BcoDI GTCTC 1 cut(s) 92
BglII AGATCT 1 cut(s) 391
BisI GCNGC 4 cut(s) 162, 450, 476, 495
BlsI GCNGC 4 cut(s) 163, 451, 477, 496
Bme1390I CCNGG 1 cut(s) 92
Bme18I GGWCC 1 cut(s) 362
BmeT110I CYCGRG 1 cut(s) 211
BmgT120I GGNCC 2 cut(s) 362, 756
BmrFI CCNGG 1 cut(s) 92
BmrI ACTGGG 1 cut(s) 464
BmsI GCATC 2 cut(s) 569, 628
BmuI ACTGGG 1 cut(s) 464
BplI GAGNNNNNCTC 2 cut(s) 462, 494
Bpu10I CCTNAGC 1 cut(s) 84
BpuMI CCSGG 1 cut(s) 92
BsaAI YACGTR 1 cut(s) 725
BsaJI CCNNGG 4 cut(s) 144, 190, 212, 647
BsaWI WCCGGW 1 cut(s) 37
BsaXI ACNNNNNCTCC 4 cut(s) 101, 125, 131, 155
Bsc4I CCNNNNNNNGG 2 cut(s) 191, 728
Bse1I ACTGG 2 cut(s) 459, 749
Bse3DI GCAATG 3 cut(s) 445, 648, 675
BseDI CCNNGG 4 cut(s) 144, 190, 212, 647
BseGI GGATG 5 cut(s) 31, 124, 505, 584, 619
BseLI CCNNNNNNNGG 2 cut(s) 191, 728
BseMI GCAATG 3 cut(s) 445, 648, 675
BseNI ACTGG 2 cut(s) 459, 749
BseRI GAGGAG 1 cut(s) 56
BseXI GCAGC 3 cut(s) 148, 487, 506
BseYI CCCAGC 1 cut(s) 370
Bsh1236I CGCG 1 cut(s) 360
BshFI GGCC 5 cut(s) 8, 156, 320, 740, 758
BsiHKCI CYCGRG 1 cut(s) 211
BsiSI CCGG 2 cut(s) 38, 92
BslI CCNNNNNNNGG 2 cut(s) 191, 728
BsmAI GTCTC 1 cut(s) 92
BsnI GGCC 5 cut(s) 8, 156, 320, 740, 758
BsoBI CYCGRG 1 cut(s) 211
Bsp143I GATC 4 cut(s) 355, 376, 391, 503
Bsp19I CCATGG 1 cut(s) 144
BspACI CCGC 3 cut(s) 360, 450, 497
BspANI GGCC 5 cut(s) 8, 156, 320, 740, 758
BspFNI CGCG 1 cut(s) 360
BspPI GGATC 1 cut(s) 511
BsrDI GCAATG 3 cut(s) 445, 648, 675
BsrI ACTGG 2 cut(s) 459, 749
BssECI CCNNGG 4 cut(s) 144, 190, 212, 647
BssMI GATC 4 cut(s) 355, 376, 391, 503
BssT1I CCWWGG 3 cut(s) 144, 190, 647
Bst4CI ACNGT 1 cut(s) 53
Bst6I CTCTTC 1 cut(s) 90
BstBAI YACGTR 1 cut(s) 725
BstDEI CTNAG 1 cut(s) 84
BstDSI CCRYGG 1 cut(s) 144
BstF5I GGATG 5 cut(s) 31, 124, 505, 584, 619
BstFNI CGCG 1 cut(s) 360
BstKTI GATC 4 cut(s) 358, 379, 394, 506
BstMAI GTCTC 1 cut(s) 92
BstMBI GATC 4 cut(s) 355, 376, 391, 503
BstMWI GCNNNNNNNGC 2 cut(s) 170, 435
BstNSI RCATGY 1 cut(s) 586
BstSCI CCNGG 1 cut(s) 90
BstUI CGCG 1 cut(s) 360
BstV1I GCAGC 3 cut(s) 148, 487, 506
BstX2I RGATCY 2 cut(s) 391, 503
BstYI RGATCY 2 cut(s) 391, 503
BsuRI GGCC 5 cut(s) 8, 156, 320, 740, 758
BtgI CCRYGG 1 cut(s) 144
BtsCI GGATG 5 cut(s) 31, 124, 505, 584, 619
BtsIMutI CAGTG 2 cut(s) 278, 452
Cfr13I GGNCC 2 cut(s) 362, 756
CpoI CGGWCCG 1 cut(s) 362
Csp6I GTAC 1 cut(s) 367
CspI CGGWCCG 1 cut(s) 362
CviAII CATG 4 cut(s) 145, 158, 322, 583
CviQI GTAC 1 cut(s) 367
DdeI CTNAG 1 cut(s) 84
DpnI GATC 4 cut(s) 357, 378, 393, 505
DpnII GATC 4 cut(s) 355, 376, 391, 503
EaeI YGGCCR 2 cut(s) 6, 154
Eam1104I CTCTTC 1 cut(s) 90
EarI CTCTTC 1 cut(s) 90
Eco130I CCWWGG 3 cut(s) 144, 190, 647
Eco47I GGWCC 1 cut(s) 362
Eco72I CACGTG 1 cut(s) 725
Eco88I CYCGRG 1 cut(s) 211
EcoT14I CCWWGG 3 cut(s) 144, 190, 647
EcoT22I ATGCAT 1 cut(s) 584
ErhI CCWWGG 3 cut(s) 144, 190, 647
FaeI CATG 4 cut(s) 148, 161, 325, 586
FatI CATG 4 cut(s) 144, 157, 321, 582
FbaI TGATCA 1 cut(s) 376
Fnu4HI GCNGC 4 cut(s) 162, 450, 476, 495
FokI GGATG 5 cut(s) 18, 111, 512, 591, 606
Fsp4HI GCNGC 4 cut(s) 162, 450, 476, 495
GluI GCNGC 4 cut(s) 162, 450, 476, 495
GsaI CCCAGC 1 cut(s) 374
HaeIII GGCC 5 cut(s) 8, 156, 320, 740, 758
HapII CCGG 2 cut(s) 38, 92
Hin1II CATG 4 cut(s) 148, 161, 325, 586
HincII GTYRAC 1 cut(s) 712
HindII GTYRAC 1 cut(s) 712
HindIII AAGCTT 2 cut(s) 336, 403
HinfI GANTC 1 cut(s) 100
HpaII CCGG 2 cut(s) 38, 92
Hpy166II GTNNAC 2 cut(s) 278, 712
Hpy188I TCNGA 3 cut(s) 463, 520, 731
Hpy188III TCNNGA 2 cut(s) 353, 395
Hpy8I GTNNAC 2 cut(s) 278, 712
HpyAV CCTTC 5 cut(s) 148, 359, 577, 631, 656
HpyCH4III ACNGT 1 cut(s) 53
HpyCH4IV ACGT 2 cut(s) 177, 724
HpyCH4V TGCA 3 cut(s) 309, 494, 582
HpyF10VI GCNNNNNNNGC 2 cut(s) 170, 435
HpyF3I CTNAG 1 cut(s) 84
HpySE526I ACGT 2 cut(s) 177, 724
Hsp92II CATG 4 cut(s) 148, 161, 325, 586
Ksp22I TGATCA 1 cut(s) 376
Kzo9I GATC 4 cut(s) 355, 376, 391, 503
LmnI GCTCC 3 cut(s) 43, 258, 568
LpnPI CCDG 8 cut(s) 51, 69, 105, 198, 365, 384, 440, 730
Lsp1109I GCAGC 3 cut(s) 148, 487, 506
LweI GCATC 2 cut(s) 569, 628
MaeII ACGT 2 cut(s) 177, 724
MalI GATC 4 cut(s) 357, 378, 393, 505
MboI GATC 4 cut(s) 355, 376, 391, 503
MboII GAAGA 1 cut(s) 107
MflI RGATCY 2 cut(s) 391, 503
MlsI TGGCCA 2 cut(s) 8, 156
MluCI AATT 2 cut(s) 513, 524
MluNI TGGCCA 2 cut(s) 8, 156
MlyI GAGTC 1 cut(s) 94
MmeI TCCRAC 1 cut(s) 709
Mox20I TGGCCA 2 cut(s) 8, 156
Mph1103I ATGCAT 1 cut(s) 584
MscI TGGCCA 2 cut(s) 8, 156
MslI CAYNNNNRTG 1 cut(s) 326
Msp20I TGGCCA 2 cut(s) 8, 156
MspA1I CMGCKG 2 cut(s) 374, 497
MspI CCGG 2 cut(s) 38, 92
MspR9I CCNGG 1 cut(s) 92
MvnI CGCG 1 cut(s) 360
MwoI GCNNNNNNNGC 2 cut(s) 170, 435
NciI CCSGG 1 cut(s) 92
NcoI CCATGG 1 cut(s) 144
NdeII GATC 4 cut(s) 355, 376, 391, 503
NlaIII CATG 4 cut(s) 148, 161, 325, 586
NsiI ATGCAT 1 cut(s) 584
NspI RCATGY 1 cut(s) 586
PkrI GCNGC 4 cut(s) 163, 451, 477, 496
PleI GAGTC 1 cut(s) 94
PmaCI CACGTG 1 cut(s) 725
PmlI CACGTG 1 cut(s) 725
PpsI GAGTC 1 cut(s) 94
Ppu21I YACGTR 1 cut(s) 725
PspCI CACGTG 1 cut(s) 725
PspFI CCCAGC 1 cut(s) 370
PspPI GGNCC 2 cut(s) 362, 756
PsuI RGATCY 2 cut(s) 391, 503
PvuII CAGCTG 1 cut(s) 374
RsaI GTAC 1 cut(s) 368
RsaNI GTAC 1 cut(s) 367
RseI CAYNNNNRTG 1 cut(s) 326
Rsr2I CGGWCCG 1 cut(s) 362
RsrII CGGWCCG 1 cut(s) 362
SatI GCNGC 4 cut(s) 162, 450, 476, 495
Sau3AI GATC 4 cut(s) 355, 376, 391, 503
Sau96I GGNCC 2 cut(s) 362, 756
SchI GAGTC 1 cut(s) 94
ScrFI CCNGG 1 cut(s) 92
SfaNI GCATC 2 cut(s) 569, 628
SinI GGWCC 1 cut(s) 362
SmiMI CAYNNNNRTG 1 cut(s) 326
Sse9I AATT 2 cut(s) 513, 524
SsiI CCGC 3 cut(s) 360, 450, 497
StyD4I CCNGG 1 cut(s) 90
StyI CCWWGG 3 cut(s) 144, 190, 647
TaaI ACNGT 1 cut(s) 53
TaiI ACGT 2 cut(s) 180, 727
TaqI TCGA 1 cut(s) 352
TasI AATT 2 cut(s) 513, 524
TauI GCSGC 1 cut(s) 452
TscAI CASTG 2 cut(s) 285, 459
TseI GCWGC 3 cut(s) 161, 475, 494
TspDTI ATGAA 4 cut(s) 537, 578, 641, 650
TspGWI ACGGA 2 cut(s) 84, 354
TspRI CASTG 2 cut(s) 285, 459
VpaK11BI GGWCC 1 cut(s) 362
XceI RCATGY 1 cut(s) 586
Zsp2I ATGCAT 1 cut(s) 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.