FvH4_3g20560

Stem-specific protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0014459
Physical Location & Seq
Forward (+)
13626748 .. 13628477
1730 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g20560.t1

Sequence Viewer

Length: 768 bp
ATGTTGGCCATTTTCCACAAGGCTTTTGCTCACCCACCGGAGGAGCTCAACAGCCCTGCTTCTCACCATGGTTCCAAGAAGCCTAAGCTGCCAGAAGAAACCCTCGAGGAGTTTCTCTCCCACCATCCTCACAACAACTTCTCAATGGGGTTTGGAGATGCTGCCGTTCTTGCTTATGTCAAACCAGACCAACCCTATTCCCTAAAACAGAGGCTGTTCTGTGGTTTCGATGATATATACTGTCTGTTTCTGGGGAGCTTGAACAATCTGTGTATGCTGAATAAGCAGTATGGTCTGACAAAGAATACCAATGAGGCCATGTTTGTGATTGAAGCTTATCGGACCCTCCGAGACAGGGGTCCGTACCCGGCTGACCAAGTTGTTAAGGATCTCGATGGAAGCTTTGCCTTTGTTGTCTATGACAGCAAGGTTGGCAGTGTCTTCACTGCACTGGGTTCTGATGGGGGAGTACAATTGTATTGGGGTATTGCAGCTGATGGTTCTGTGGTGATTTCTGATGAATTACATGTCATAAAAGAAGGATGTGCTAAATCATATGCTCCGTTCCCAAAAGGTTGCTTCTTTCATAGTGAGGGAGGATTGATGAGCTTTGAGCATCCGATGAATAAGATGAAGGCAATGCCCAGAATTGATAGTGAAGGTGCCATGTGTGGAGCTAACTTCAACGTGGACAAATTCACTAGGATCAACAGTATTCCACGAGTCGGCAGCGAAGCAAACTGGAGTGCTGAGTGGACATCACACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3700

Protein Analysis

256

Amino Acids

28.27

Weight (kDa)

5.98

Isoelectric Point (pI)

29.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3700 PF12481 2 - 230 3e-109 Aluminium induced protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014459)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27450
fragaria_vesca FvH4_3g20560
malus_domestica MD03G1224100.v1.1 MD11G1241900.v1.1
prunus_persica Prupe.4G185400_v2.0.a1
pyrus_communis pycom03g17250 pycom11g21360
rosa_chinensis RchiOBHm_Chr5g0034571
rosa_laevigata RLG00000033555
rosa_multiflora Rmu_sc0003126.1_g000004
rosa_roxburghii Rroxscaffold_1G00045760
rosa_rugosa Rorug05G0146500
rosa_samantha Rh5AG238900 Rh5BG239400 Rh5CG269900
rosa_wichuraiana Rw5G021780 Rw5G021870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 104
AccB1I GGYRCC 1 cut(s) 662
AclWI GGATC 2 cut(s) 396, 713
AcoI YGGCCR 1 cut(s) 6
AcsI RAATTY 1 cut(s) 695
AfaI GTAC 2 cut(s) 365, 471
AfiI CCNNNNNNNGG 3 cut(s) 40, 355, 725
AflIII ACRYGT 1 cut(s) 526
AgsI TTSAA 3 cut(s) 262, 332, 685
AluBI AGCT 8 cut(s) 46, 88, 258, 335, 402, 494, 609, 677
AluI AGCT 8 cut(s) 46, 88, 258, 335, 402, 494, 609, 677
Alw21I GWGCWC 1 cut(s) 48
Alw26I GTCTC 1 cut(s) 345
AlwI GGATC 2 cut(s) 396, 713
AlwNI CAGNNNCTG 1 cut(s) 214
Ama87I CYCGRG 1 cut(s) 104
AoxI GGCC 2 cut(s) 6, 315
ApeKI GCWGC 4 cut(s) 88, 161, 491, 729
ApoI RAATTY 1 cut(s) 695
AspS9I GGNCC 2 cut(s) 342, 359
AsuC2I CCSGG 1 cut(s) 368
AsuHPI GGTGA 3 cut(s) 23, 56, 520
AvaI CYCGRG 1 cut(s) 104
AvaII GGWCC 2 cut(s) 342, 359
BalI TGGCCA 1 cut(s) 8
BanI GGYRCC 1 cut(s) 662
BanII GRGCYC 1 cut(s) 48
BauI CACGAG 1 cut(s) 720
BbsI GAAGAC 1 cut(s) 433
Bbv12I GWGCWC 1 cut(s) 48
BbvI GCAGC 4 cut(s) 75, 148, 503, 741
BccI CCATC 4 cut(s) 132, 389, 455, 491
BceAI ACGGC 1 cut(s) 149
BcnI CCSGG 1 cut(s) 368
BcoDI GTCTC 1 cut(s) 345
BfaI CTAG 1 cut(s) 702
BisI GCNGC 4 cut(s) 89, 162, 492, 730
BlsI GCNGC 4 cut(s) 90, 163, 493, 731
Bme1390I CCNGG 1 cut(s) 368
Bme18I GGWCC 2 cut(s) 342, 359
BmeT110I CYCGRG 1 cut(s) 104
BmgT120I GGNCC 2 cut(s) 342, 359
BmiI GGNNCC 4 cut(s) 73, 344, 360, 664
BmrFI CCNGG 1 cut(s) 368
BmrI ACTGGG 1 cut(s) 461
BmsI GCATC 2 cut(s) 148, 625
BmuI ACTGGG 1 cut(s) 461
BoxI GACNNNNGTC 1 cut(s) 357
BpiI GAAGAC 1 cut(s) 433
BplI GAGNNNNNCTC 2 cut(s) 101, 133
BpmI CTGGAG 1 cut(s) 763
Bpu10I CCTNAGC 1 cut(s) 84
BpuMI CCSGG 1 cut(s) 368
BsaJI CCNNGG 1 cut(s) 67
BsaWI WCCGGW 1 cut(s) 37
Bsc4I CCNNNNNNNGG 3 cut(s) 40, 355, 725
Bse1I ACTGG 2 cut(s) 456, 746
Bse3DI GCAATG 1 cut(s) 645
BseDI CCNNGG 1 cut(s) 67
BseGI GGATG 3 cut(s) 124, 548, 616
BseLI CCNNNNNNNGG 3 cut(s) 40, 355, 725
BseMI GCAATG 1 cut(s) 645
BseMII CTCAG 1 cut(s) 741
BseNI ACTGG 2 cut(s) 456, 746
BseRI GAGGAG 2 cut(s) 56, 122
BseXI GCAGC 4 cut(s) 75, 148, 503, 741
BsgI GTGCAG 1 cut(s) 432
BshFI GGCC 2 cut(s) 8, 317
BshNI GGYRCC 1 cut(s) 662
BsiHKAI GWGCWC 1 cut(s) 48
BsiHKCI CYCGRG 1 cut(s) 104
BsiSI CCGG 2 cut(s) 38, 368
BslI CCNNNNNNNGG 3 cut(s) 40, 355, 725
BsmAI GTCTC 1 cut(s) 345
BsnI GGCC 2 cut(s) 8, 317
BsoBI CYCGRG 1 cut(s) 104
Bsp1286I GDGCHC 1 cut(s) 48
Bsp143I GATC 2 cut(s) 388, 705
Bsp19I CCATGG 1 cut(s) 67
BspANI GGCC 2 cut(s) 8, 317
BspCNI CTCAG 1 cut(s) 742
BspLI GGNNCC 4 cut(s) 73, 344, 360, 664
BspPI GGATC 2 cut(s) 396, 713
BspT107I GGYRCC 1 cut(s) 662
BsrDI GCAATG 1 cut(s) 645
BsrI ACTGG 2 cut(s) 456, 746
BssECI CCNNGG 1 cut(s) 67
BssMI GATC 2 cut(s) 388, 705
BssSI CACGAG 1 cut(s) 720
BssT1I CCWWGG 1 cut(s) 67
Bst2BI CACGAG 1 cut(s) 720
Bst4CI ACNGT 2 cut(s) 242, 713
BstDEI CTNAG 2 cut(s) 84, 750
BstDSI CCRYGG 1 cut(s) 67
BstF5I GGATG 3 cut(s) 124, 548, 616
BstKTI GATC 2 cut(s) 391, 708
BstMAI GTCTC 1 cut(s) 345
BstMBI GATC 2 cut(s) 388, 705
BstMWI GCNNNNNNNGC 4 cut(s) 88, 170, 283, 432
BstNSI RCATGY 1 cut(s) 530
BstPAI GACNNNNGTC 1 cut(s) 357
BstSCI CCNGG 1 cut(s) 366
BstV1I GCAGC 4 cut(s) 75, 148, 503, 741
BstV2I GAAGAC 1 cut(s) 433
BstX2I RGATCY 1 cut(s) 388
BstYI RGATCY 1 cut(s) 388
BsuRI GGCC 2 cut(s) 8, 317
BtgI CCRYGG 1 cut(s) 67
BtsCI GGATG 3 cut(s) 124, 548, 616
BtsI GCAGTG 2 cut(s) 442, 444
BtsIMutI CAGTG 3 cut(s) 442, 444, 449
CaiI CAGNNNCTG 1 cut(s) 214
Cfr13I GGNCC 2 cut(s) 342, 359
Csp6I GTAC 2 cut(s) 364, 470
CviAII CATG 4 cut(s) 68, 319, 527, 667
CviQI GTAC 2 cut(s) 364, 470
DdeI CTNAG 2 cut(s) 84, 750
DpnI GATC 2 cut(s) 390, 707
DpnII GATC 2 cut(s) 388, 705
EaeI YGGCCR 1 cut(s) 6
Ecl136II GAGCTC 1 cut(s) 46
Eco130I CCWWGG 1 cut(s) 67
Eco24I GRGCYC 1 cut(s) 48
Eco47I GGWCC 2 cut(s) 342, 359
Eco53kI GAGCTC 1 cut(s) 46
Eco88I CYCGRG 1 cut(s) 104
EcoICRI GAGCTC 1 cut(s) 46
EcoT14I CCWWGG 1 cut(s) 67
EcoT38I GRGCYC 1 cut(s) 48
ErhI CCWWGG 1 cut(s) 67
FaeI CATG 4 cut(s) 71, 322, 530, 670
FatI CATG 4 cut(s) 67, 318, 526, 666
FauNDI CATATG 1 cut(s) 556
Fnu4HI GCNGC 4 cut(s) 89, 162, 492, 730
FokI GGATG 3 cut(s) 111, 555, 603
FriOI GRGCYC 1 cut(s) 48
Fsp4HI GCNGC 4 cut(s) 89, 162, 492, 730
FspBI CTAG 1 cut(s) 702
GluI GCNGC 4 cut(s) 89, 162, 492, 730
GsuI CTGGAG 1 cut(s) 763
HaeIII GGCC 2 cut(s) 8, 317
HapII CCGG 2 cut(s) 38, 368
Hin1II CATG 4 cut(s) 71, 322, 530, 670
HindIII AAGCTT 2 cut(s) 333, 400
HinfI GANTC 1 cut(s) 723
HpaII CCGG 2 cut(s) 38, 368
HphI GGTGA 3 cut(s) 23, 56, 520
Hpy166II GTNNAC 2 cut(s) 691, 756
Hpy188I TCNGA 6 cut(s) 297, 342, 350, 460, 517, 621
Hpy188III TCNNGA 1 cut(s) 392
Hpy8I GTNNAC 2 cut(s) 691, 756
HpyAV CCTTC 3 cut(s) 533, 628, 653
HpyCH4III ACNGT 2 cut(s) 242, 713
HpyCH4IV ACGT 1 cut(s) 687
HpyCH4V TGCA 2 cut(s) 449, 491
HpyF10VI GCNNNNNNNGC 4 cut(s) 88, 170, 283, 432
HpyF3I CTNAG 2 cut(s) 84, 750
HpySE526I ACGT 1 cut(s) 687
Hsp92II CATG 4 cut(s) 71, 322, 530, 670
Kzo9I GATC 2 cut(s) 388, 705
LmnI GCTCC 4 cut(s) 43, 255, 565, 674
Lsp1109I GCAGC 4 cut(s) 75, 148, 503, 741
LweI GCATC 2 cut(s) 148, 625
MaeI CTAG 1 cut(s) 702
MaeII ACGT 1 cut(s) 687
MalI GATC 2 cut(s) 390, 707
MboI GATC 2 cut(s) 388, 705
MboII GAAGA 2 cut(s) 107, 433
MfeI CAATTG 1 cut(s) 473
MflI RGATCY 1 cut(s) 388
MhlI GDGCHC 1 cut(s) 48
MlsI TGGCCA 1 cut(s) 8
MluCI AATT 4 cut(s) 473, 521, 648, 695
MluNI TGGCCA 1 cut(s) 8
MlyI GAGTC 1 cut(s) 732
MnlI CCTC 9 cut(s) 34, 100, 113, 138, 204, 307, 356, 586, 590
Mox20I TGGCCA 1 cut(s) 8
MscI TGGCCA 1 cut(s) 8
MseI TTAA 1 cut(s) 384
MslI CAYNNNNRTG 1 cut(s) 323
Msp20I TGGCCA 1 cut(s) 8
MspA1I CMGCKG 1 cut(s) 494
MspI CCGG 2 cut(s) 38, 368
MspR9I CCNGG 1 cut(s) 368
MunI CAATTG 1 cut(s) 473
MwoI GCNNNNNNNGC 4 cut(s) 88, 170, 283, 432
NciI CCSGG 1 cut(s) 368
NcoI CCATGG 1 cut(s) 67
NdeI CATATG 1 cut(s) 556
NdeII GATC 2 cut(s) 388, 705
NlaIII CATG 4 cut(s) 71, 322, 530, 670
NlaIV GGNNCC 4 cut(s) 73, 344, 360, 664
NspI RCATGY 1 cut(s) 530
PaeR7I CTCGAG 1 cut(s) 104
PciI ACATGT 1 cut(s) 526
PcsI WCGNNNNNNNCGW 1 cut(s) 346
PkrI GCNGC 4 cut(s) 90, 163, 493, 731
PleI GAGTC 1 cut(s) 731
PpsI GAGTC 1 cut(s) 731
PscI ACATGT 1 cut(s) 526
PshAI GACNNNNGTC 1 cut(s) 357
Psp124BI GAGCTC 1 cut(s) 48
PspN4I GGNNCC 4 cut(s) 73, 344, 360, 664
PspPI GGNCC 2 cut(s) 342, 359
PspXI VCTCGAGB 1 cut(s) 104
PstNI CAGNNNCTG 1 cut(s) 214
PsuI RGATCY 1 cut(s) 388
PvuII CAGCTG 1 cut(s) 494
RsaI GTAC 2 cut(s) 365, 471
RsaNI GTAC 2 cut(s) 364, 470
RseI CAYNNNNRTG 1 cut(s) 323
SacI GAGCTC 1 cut(s) 48
SaqAI TTAA 1 cut(s) 384
SatI GCNGC 4 cut(s) 89, 162, 492, 730
Sau3AI GATC 2 cut(s) 388, 705
Sau96I GGNCC 2 cut(s) 342, 359
SchI GAGTC 1 cut(s) 732
ScrFI CCNGG 1 cut(s) 368
SduI GDGCHC 1 cut(s) 48
SfaNI GCATC 2 cut(s) 148, 625
Sfr274I CTCGAG 1 cut(s) 104
SinI GGWCC 2 cut(s) 342, 359
SlaI CTCGAG 1 cut(s) 104
SmiMI CAYNNNNRTG 1 cut(s) 323
SmlI CTYRAG 1 cut(s) 104
SmoI CTYRAG 1 cut(s) 104
Sse9I AATT 4 cut(s) 473, 521, 648, 695
SspMI CTAG 1 cut(s) 702
SstI GAGCTC 1 cut(s) 48
StyD4I CCNGG 1 cut(s) 366
StyI CCWWGG 1 cut(s) 67
TaaI ACNGT 2 cut(s) 242, 713
TaiI ACGT 1 cut(s) 690
TaqI TCGA 3 cut(s) 105, 228, 393
TasI AATT 4 cut(s) 473, 521, 648, 695
TatI WGTACW 1 cut(s) 469
Tru1I TTAA 1 cut(s) 384
Tru9I TTAA 1 cut(s) 384
TscAI CASTG 3 cut(s) 442, 451, 456
TseI GCWGC 4 cut(s) 88, 161, 491, 729
TspDTI ATGAA 4 cut(s) 534, 575, 638, 647
TspGWI ACGGA 2 cut(s) 351, 552
TspRI CASTG 3 cut(s) 442, 451, 456
VpaK11BI GGWCC 2 cut(s) 342, 359
XapI RAATTY 1 cut(s) 695
XceI RCATGY 1 cut(s) 530
XhoI CTCGAG 1 cut(s) 104
XspI CTAG 1 cut(s) 702
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.