AT4G32630

ADP-ribosylation factor GTPase-activating protein AGD14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0006494
Physical Location & Seq
Forward (+)
15737918 .. 15741741
3824 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G32630.3

Sequence Viewer

Length: 1884 bp
ATGAAGGAAGACGAGCGAACCGAAAAGGCTATCCGGAGTTTATTGAAGCTTCCTGAGAACCGGAGATGCATCAATTGCAATAGTCTGGGTCCACAATATGTATGCTCAACTTTCTGGACTTTTGTCTGCGTTAACTGCAGCGGAATCCACCGAGAATTCACACATCGTGTTAAATCTGTATCAATGGCCAAGTTCACAGCAGACGAAGTTAGTGCACTTCGCGCAGGAGGAAACGAGCGAGCCAGACAAATTTATTTTAAAGAATGGGATGCTCACCGTGATGGTTACCCCGATCGCAGTAATATTTTCAAGCTCCGAGATTTCATCAGGAGTGTTTATGTGGACAAAAGATACAGTAGCAGTGACAAGATCTCACAGCAGAAATCGGATGTCACAGAAGATTACAGGGAAAGTAAGAAGACCAGTGCACATGTTCTCGGATCTAGAAGTTTACACTCTGTGGATAAATCTGACATCGAAAGATCTAGTGCTGCTGGGAGGAGTGGAAGTGAATCTCTAAGGTTTTATTTTGATGACAAGAACCATAAACAACAACATGTTACGCATAATCCAAGGTCTAGAGGTTTACCAAAGAGTCCTATTCGCTTTGAGATAGTCGATGATAGGTTTAGAGACGATGGGAGTGTTAAGAGATATGATGCACGTAAAGATTCAAGAGGAAGCTCCAAATCACTTGACCTCTCAAGTAACAAAGACATGCCAAGCTTTCCCATTGTACGCCATACCAGCGAACTCAATATTGTAAAAGTTGAGAAAAAGAAGGATCCAGTGAATAATCAGATGACTGCATCATCTGAGAAAATGGAAATTCCTAGAAGTTTAATTGATGATGTACCAGTTTCAGAACTATCTGATGAAGGCATTATTAAGAATTCAAGTGAAATTCCTGCATCTCTAAAGACCACTGAGGAGCCAGCTCCTAATTCTTTGGAAGCTTTGTTGTTTGGATCCTCTGTTCCTTCTGTTGTCCCTGGAACAAACAACTATGAACTATGGAACACAAGTGATATTTCCTCTACTGAAAACTATACCGCAGTTAACCTCGGGACCCAGACAATGCCAGGAATACCAGACAGTGTTACCTCTTTTGCCACATCACCAACAACTGCACATGCTCATTCCGGTTCTTCAGGTCCAGTGGTACCTGTTGCTCCTGATAACTTAAATACAAAAGAGACGGCTACACTTGCAAATAACCAGGGACCATCAGATTTCTCTATGGAACAGACAACACTGGCTATCACAGATTATGCTCATGGAGTTGGATCTGAGCATCATCAAGATGATGAGACACAATCTAGCATAAGAAAGGCGCTTCCTGAGGACCTTTTCACTGGAGGCTTCTCATTTGCCCCTCAGCAAGTTCATGGTCAACATCATGGCATGGGATATGGCATGCAATATTATCAATATCCTGTGGCTATGGGAGCCTTAACATATACAGCAAAAGCAGCAAATCCCTTTGATCTTAGCTATGATGATACAGCTCCAAACCAAACACCACAATTTCCTACTATGGCATATGTGCAAGGAGGAGGCTTACCTCACGTGTCATCTCCAATAGGGTATTCAGATTCTTCAAGTCCAGCTGCAGATTCCATTGGACTGATGACCTCTCAGTCACCTTTTCATGCAACCGCTTTATCCCCAAACTCTCCAGCTCTTGCATCCCATCTTTCCCCTGGTGCACTAATGGGGCAGCAATCACAGGTGAATATGTCCCCATCATTCAGGCAAGAGTATAGTGGTCTTGGTACAGAAGGAAATACGTTCAACGGAGTACATACATTTCATCAAGCAAACAATGGATATCCTTCTGCAAACCCAAATGCTTATGTATCCAGAGGAAATCCCTTTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArfGap

Protein Analysis

627

Amino Acids

68.8

Weight (kDa)

6.13

Isoelectric Point (pI)

52.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArfGap PF01412 9 - 120 3.4e-28 Putative GTPase activating protein for Arf
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1639
Acc65I GGTACC 1 cut(s) 1162
AccB1I GGYRCC 1 cut(s) 1162
AccII CGCG 1 cut(s) 222
AccIII TCCGGA 1 cut(s) 33
AciI CCGC 3 cut(s) 141, 1053, 1659
AclWI GGATC 6 cut(s) 448, 779, 792, 963, 976, 1294
AcoI YGGCCR 1 cut(s) 186
AcsI RAATTY 5 cut(s) 155, 249, 828, 892, 903
AcuI CTGAAG 1 cut(s) 1134
AcvI CACGTG 1 cut(s) 1570
AdeI CACNNNGTG 2 cut(s) 167, 460
AfaI GTAC 5 cut(s) 738, 855, 1164, 1777, 1803
AflIII ACRYGT 3 cut(s) 430, 556, 1569
AgsI TTSAA 7 cut(s) 46, 310, 675, 897, 1602, 1795, 1880
AjnI CCWGG 4 cut(s) 991, 1081, 1218, 1702
AjuI GAANNNNNNNTTGG 2 cut(s) 1573, 1605
AloI GAACNNNNNNTCC 2 cut(s) 960, 992
Alw21I GWGCWC 3 cut(s) 217, 430, 1711
Alw26I GTCTC 3 cut(s) 627, 1190, 1304
Alw44I GTGCAC 3 cut(s) 213, 426, 1707
AlwI GGATC 6 cut(s) 448, 779, 792, 963, 976, 1294
Ama87I CYCGRG 1 cut(s) 1064
Aor13HI TCCGGA 1 cut(s) 33
AoxI GGCC 1 cut(s) 186
ApaLI GTGCAC 3 cut(s) 213, 426, 1707
ApeKI GCWGC 5 cut(s) 138, 491, 1472, 1610, 1720
ApoI RAATTY 5 cut(s) 155, 249, 828, 892, 903
Asp718I GGTACC 1 cut(s) 1162
AspLEI GCGC 2 cut(s) 224, 1336
AspS9I GGNCC 5 cut(s) 89, 1068, 1154, 1223, 1345
AsuHPI GGTGA 4 cut(s) 266, 1110, 1635, 1744
AvaI CYCGRG 1 cut(s) 1064
AvaII GGWCC 5 cut(s) 89, 1068, 1154, 1223, 1345
AxyI CCTNAGG 1 cut(s) 1341
BaeGI GKGCMC 3 cut(s) 217, 430, 1711
BalI TGGCCA 1 cut(s) 188
BamHI GGATCC 2 cut(s) 784, 968
BanI GGYRCC 1 cut(s) 1162
BbrPI CACGTG 1 cut(s) 1570
BbsI GAAGAC 2 cut(s) 15, 425
Bbv12I GWGCWC 3 cut(s) 217, 430, 1711
BbvCI CCTCAGC 1 cut(s) 1377
BbvI GCAGC 5 cut(s) 150, 478, 1484, 1597, 1732
BccI CCATC 5 cut(s) 275, 632, 1234, 1701, 1753
BceAI ACGGC 1 cut(s) 1215
BcgI CGANNNNNNTGC 2 cut(s) 194, 228
BciT130I CCWGG 4 cut(s) 993, 1083, 1220, 1704
BciVI GTATCC 1 cut(s) 1870
BcoDI GTCTC 3 cut(s) 627, 1190, 1304
BfaI CTAG 5 cut(s) 444, 486, 579, 834, 1320
BfmI CTRYAG 2 cut(s) 136, 1611
BfoI RGCGCY 1 cut(s) 1337
BfuI GTATCC 1 cut(s) 1870
BglII AGATCT 2 cut(s) 369, 482
BisI GCNGC 5 cut(s) 139, 492, 1473, 1611, 1721
BlsI GCNGC 5 cut(s) 140, 493, 1474, 1612, 1722
Bme1390I CCNGG 4 cut(s) 993, 1083, 1220, 1704
Bme18I GGWCC 5 cut(s) 89, 1068, 1154, 1223, 1345
BmeT110I CYCGRG 1 cut(s) 1064
BmgT120I GGNCC 5 cut(s) 89, 1068, 1154, 1223, 1345
BmiI GGNNCC 9 cut(s) 90, 786, 933, 970, 1069, 1070, 1164, 1224, 1450
BmrFI CCNGG 4 cut(s) 993, 1083, 1220, 1704
BmsI GCATC 8 cut(s) 56, 78, 259, 649, 818, 920, 1303, 1697
BoxI GACNNNNGTC 1 cut(s) 122
BpiI GAAGAC 2 cut(s) 15, 425
BpmI CTGGAG 2 cut(s) 1377, 1662
Bpu10I CCTNAGC 1 cut(s) 1377
BpuEI CTTGAG 1 cut(s) 688
BsaAI YACGTR 2 cut(s) 665, 1570
BsaJI CCNNGG 5 cut(s) 572, 991, 1063, 1219, 1702
BsaWI WCCGGW 3 cut(s) 33, 60, 1142
BsaXI ACNNNNNCTCC 2 cut(s) 493, 523
Bse1I ACTGG 6 cut(s) 423, 788, 857, 1157, 1260, 1360
Bse21I CCTNAGG 1 cut(s) 1341
BseAI TCCGGA 1 cut(s) 33
BseBI CCWGG 4 cut(s) 993, 1083, 1220, 1704
BseDI CCNNGG 5 cut(s) 572, 991, 1063, 1219, 1702
BseGI GGATG 3 cut(s) 274, 394, 1688
BseMII CTCAG 7 cut(s) 45, 807, 918, 1281, 1332, 1391, 1652
BseNI ACTGG 6 cut(s) 423, 788, 857, 1157, 1260, 1360
BseRI GAGGAG 3 cut(s) 514, 944, 1569
BseSI GKGCMC 3 cut(s) 217, 430, 1711
BseXI GCAGC 5 cut(s) 150, 478, 1484, 1597, 1732
BseYI CCCAGC 1 cut(s) 494
BsgI GTGCAG 1 cut(s) 1113
Bsh1236I CGCG 1 cut(s) 222
Bsh1285I CGRYCG 1 cut(s) 295
BshFI GGCC 1 cut(s) 188
BshNI GGYRCC 1 cut(s) 1162
BsiEI CGRYCG 1 cut(s) 295
BsiHKAI GWGCWC 3 cut(s) 217, 430, 1711
BsiHKCI CYCGRG 1 cut(s) 1064
BsiSI CCGG 3 cut(s) 34, 61, 1143
BslFI GGGAC 4 cut(s) 974, 1081, 1236, 1726
BsmAI GTCTC 3 cut(s) 627, 1190, 1304
BsmBI CGTCTC 2 cut(s) 627, 1190
BsmFI GGGAC 4 cut(s) 974, 1081, 1236, 1726
BsnI GGCC 1 cut(s) 188
BsoBI CYCGRG 1 cut(s) 1064
Bsp1286I GDGCHC 3 cut(s) 217, 430, 1711
Bsp13I TCCGGA 1 cut(s) 33
Bsp143I GATC 8 cut(s) 292, 369, 440, 482, 784, 968, 1286, 1486
BspACI CCGC 3 cut(s) 141, 1053, 1659
BspANI GGCC 1 cut(s) 188
BspCNI CTCAG 7 cut(s) 46, 808, 919, 1282, 1333, 1390, 1651
BspEI TCCGGA 1 cut(s) 33
BspFNI CGCG 1 cut(s) 222
BspLI GGNNCC 9 cut(s) 90, 786, 933, 970, 1069, 1070, 1164, 1224, 1450
BspMAI CTGCAG 2 cut(s) 140, 1615
BspPI GGATC 6 cut(s) 448, 779, 792, 963, 976, 1294
BspT107I GGYRCC 1 cut(s) 1162
BsrI ACTGG 6 cut(s) 423, 788, 857, 1157, 1260, 1360
BssECI CCNNGG 5 cut(s) 572, 991, 1063, 1219, 1702
BssMI GATC 8 cut(s) 292, 369, 440, 482, 784, 968, 1286, 1486
BssT1I CCWWGG 1 cut(s) 572
Bst2UI CCWGG 4 cut(s) 993, 1083, 1220, 1704
Bst4CI ACNGT 3 cut(s) 278, 356, 1097
BstAPI GCANNNNNTGC 1 cut(s) 75
BstBAI YACGTR 2 cut(s) 665, 1570
BstC8I GCNNGC 3 cut(s) 240, 936, 1418
BstDEI CTNAG 9 cut(s) 54, 518, 816, 927, 1290, 1341, 1377, 1490, 1638
BstEII GGTNACC 1 cut(s) 284
BstF5I GGATG 3 cut(s) 274, 394, 1688
BstFNI CGCG 1 cut(s) 222
BstH2I RGCGCY 1 cut(s) 1337
BstHHI GCGC 2 cut(s) 224, 1336
BstKTI GATC 8 cut(s) 295, 372, 443, 485, 787, 971, 1289, 1489
BstMAI GTCTC 3 cut(s) 627, 1190, 1304
BstMBI GATC 8 cut(s) 292, 369, 440, 482, 784, 968, 1286, 1486
BstMCI CGRYCG 1 cut(s) 295
BstMWI GCNNNNNNNGC 7 cut(s) 75, 135, 221, 747, 1208, 1448, 1472
BstNI CCWGG 4 cut(s) 993, 1083, 1220, 1704
BstNSI RCATGY 5 cut(s) 434, 560, 721, 1136, 1420
BstPAI GACNNNNGTC 1 cut(s) 122
BstPI GGTNACC 1 cut(s) 284
BstSCI CCNGG 4 cut(s) 991, 1081, 1218, 1702
BstSFI CTRYAG 2 cut(s) 136, 1611
BstSLI GKGCMC 3 cut(s) 217, 430, 1711
BstUI CGCG 1 cut(s) 222
BstV1I GCAGC 5 cut(s) 150, 478, 1484, 1597, 1732
BstV2I GAAGAC 2 cut(s) 15, 425
BstX2I RGATCY 6 cut(s) 369, 440, 482, 784, 968, 1286
BstYI RGATCY 6 cut(s) 369, 440, 482, 784, 968, 1286
Bsu36I CCTNAGG 1 cut(s) 1341
BsuI GTATCC 1 cut(s) 1870
BsuRI GGCC 1 cut(s) 188
BtsCI GGATG 3 cut(s) 274, 394, 1688
BtsI GCAGTG 1 cut(s) 367
BtsIMutI CAGTG 8 cut(s) 367, 430, 795, 924, 1102, 1164, 1253, 1353
Cac8I GCNNGC 3 cut(s) 240, 936, 1418
CfoI GCGC 2 cut(s) 224, 1336
Cfr13I GGNCC 5 cut(s) 89, 1068, 1154, 1223, 1345
Csp6I GTAC 5 cut(s) 737, 854, 1163, 1776, 1802
CviQI GTAC 5 cut(s) 737, 854, 1163, 1776, 1802
DdeI CTNAG 9 cut(s) 54, 518, 816, 927, 1290, 1341, 1377, 1490, 1638
DpnI GATC 8 cut(s) 294, 371, 442, 484, 786, 970, 1288, 1488
DpnII GATC 8 cut(s) 292, 369, 440, 482, 784, 968, 1286, 1486
DraI TTTAAA 1 cut(s) 259
DraIII CACNNNGTG 2 cut(s) 167, 460
DrdI GACNNNNNNGTC 1 cut(s) 1639
DseDI GACNNNNNNGTC 1 cut(s) 1639
EaeI YGGCCR 1 cut(s) 186
Eco130I CCWWGG 1 cut(s) 572
Eco32I GATATC 1 cut(s) 1832
Eco47I GGWCC 5 cut(s) 89, 1068, 1154, 1223, 1345
Eco57I CTGAAG 1 cut(s) 1134
Eco72I CACGTG 1 cut(s) 1570
Eco81I CCTNAGG 1 cut(s) 1341
Eco88I CYCGRG 1 cut(s) 1064
Eco91I GGTNACC 1 cut(s) 284
EcoO109I RGGNCCY 2 cut(s) 1068, 1345
EcoO65I GGTNACC 1 cut(s) 284
EcoRI GAATTC 2 cut(s) 155, 892
EcoRII CCWGG 4 cut(s) 991, 1081, 1218, 1702
EcoRV GATATC 1 cut(s) 1832
EcoT14I CCWWGG 1 cut(s) 572
EcoT22I ATGCAT 1 cut(s) 71
ErhI CCWWGG 1 cut(s) 572
Esp3I CGTCTC 2 cut(s) 627, 1190
FaqI GGGAC 4 cut(s) 974, 1081, 1236, 1726
FauNDI CATATG 1 cut(s) 1543
Fnu4HI GCNGC 5 cut(s) 139, 492, 1473, 1611, 1721
FokI GGATG 3 cut(s) 281, 401, 1675
Fsp4HI GCNGC 5 cut(s) 139, 492, 1473, 1611, 1721
FspBI CTAG 5 cut(s) 444, 486, 579, 834, 1320
GlaI GCGC 2 cut(s) 223, 1335
GluI GCNGC 5 cut(s) 139, 492, 1473, 1611, 1721
GsaI CCCAGC 1 cut(s) 498
GsuI CTGGAG 2 cut(s) 1377, 1662
HaeII RGCGCY 1 cut(s) 1337
HaeIII GGCC 1 cut(s) 188
HapII CCGG 3 cut(s) 34, 61, 1143
HhaI GCGC 2 cut(s) 224, 1336
Hin6I GCGC 2 cut(s) 222, 1334
HinP1I GCGC 2 cut(s) 222, 1334
HincII GTYRAC 3 cut(s) 133, 1060, 1394
HindII GTYRAC 3 cut(s) 133, 1060, 1394
HindIII AAGCTT 3 cut(s) 47, 724, 954
HinfI GANTC 6 cut(s) 144, 512, 595, 671, 1595, 1616
HpaI GTTAAC 2 cut(s) 133, 1060
HpaII CCGG 3 cut(s) 34, 61, 1143
HphI GGTGA 4 cut(s) 266, 1110, 1635, 1744
HpyAV CCTTC 5 cut(s) 775, 872, 990, 1775, 1845
HpyCH4III ACNGT 3 cut(s) 278, 356, 1097
HpyCH4IV ACGT 3 cut(s) 664, 1569, 1790
HpyF10VI GCNNNNNNNGC 7 cut(s) 75, 135, 221, 747, 1208, 1448, 1472
HpyF3I CTNAG 9 cut(s) 54, 518, 816, 927, 1290, 1341, 1377, 1490, 1638
HpySE526I ACGT 3 cut(s) 664, 1569, 1790
HspAI GCGC 2 cut(s) 222, 1334
KflI GGGWCCC 1 cut(s) 1068
Kpn2I TCCGGA 1 cut(s) 33
KpnI GGTACC 1 cut(s) 1166
KspAI GTTAAC 2 cut(s) 133, 1060
Kzo9I GATC 8 cut(s) 292, 369, 440, 482, 784, 968, 1286, 1486
LmnI GCTCC 7 cut(s) 318, 689, 931, 943, 1177, 1448, 1513
Lsp1109I GCAGC 5 cut(s) 150, 478, 1484, 1597, 1732
LweI GCATC 8 cut(s) 56, 78, 259, 649, 818, 920, 1303, 1697
MaeI CTAG 5 cut(s) 444, 486, 579, 834, 1320
MaeII ACGT 3 cut(s) 664, 1569, 1790
MaeIII GTNAC 7 cut(s) 284, 362, 391, 559, 707, 1099, 1641
MalI GATC 8 cut(s) 294, 371, 442, 484, 786, 970, 1288, 1488
MboI GATC 8 cut(s) 292, 369, 440, 482, 784, 968, 1286, 1486
MboII GAAGA 5 cut(s) 20, 410, 430, 1140, 1590
MfeI CAATTG 1 cut(s) 73
MflI RGATCY 6 cut(s) 369, 440, 482, 784, 968, 1286
MhlI GDGCHC 3 cut(s) 217, 430, 1711
MlsI TGGCCA 1 cut(s) 188
MluCI AATT 9 cut(s) 73, 155, 249, 828, 843, 892, 903, 943, 1526
MluNI TGGCCA 1 cut(s) 188
MlyI GAGTC 1 cut(s) 604
MmeI TCCRAC 1 cut(s) 1264
Mox20I TGGCCA 1 cut(s) 188
Mph1103I ATGCAT 1 cut(s) 71
MroI TCCGGA 1 cut(s) 33
MscI TGGCCA 1 cut(s) 188
MseI TTAA 9 cut(s) 132, 171, 258, 648, 842, 888, 1059, 1184, 1454
MslI CAYNNNNRTG 2 cut(s) 279, 1302
Msp20I TGGCCA 1 cut(s) 188
MspA1I CMGCKG 2 cut(s) 141, 1610
MspI CCGG 3 cut(s) 34, 61, 1143
MspR9I CCNGG 4 cut(s) 993, 1083, 1220, 1704
MunI CAATTG 1 cut(s) 73
MvaI CCWGG 4 cut(s) 993, 1083, 1220, 1704
MvnI CGCG 1 cut(s) 222
MwoI GCNNNNNNNGC 7 cut(s) 75, 135, 221, 747, 1208, 1448, 1472
NdeI CATATG 1 cut(s) 1543
NdeII GATC 8 cut(s) 292, 369, 440, 482, 784, 968, 1286, 1486
NlaIV GGNNCC 9 cut(s) 90, 786, 933, 970, 1069, 1070, 1164, 1224, 1450
NmuCI GTSAC 3 cut(s) 362, 391, 1641
NsiI ATGCAT 1 cut(s) 71
NspI RCATGY 5 cut(s) 434, 560, 721, 1136, 1420
PaeI GCATGC 1 cut(s) 1420
PciI ACATGT 2 cut(s) 430, 556
PcsI WCGNNNNNNNCGW 1 cut(s) 18
PfeI GAWTC 5 cut(s) 144, 512, 671, 1595, 1616
PkrI GCNGC 5 cut(s) 140, 493, 1474, 1612, 1722
Ple19I CGATCG 1 cut(s) 295
PleI GAGTC 1 cut(s) 603
PmaCI CACGTG 1 cut(s) 1570
PmlI CACGTG 1 cut(s) 1570
PpsI GAGTC 1 cut(s) 603
Ppu21I YACGTR 2 cut(s) 665, 1570
PpuMI RGGWCCY 2 cut(s) 1068, 1345
PscI ACATGT 2 cut(s) 430, 556
PshAI GACNNNNGTC 1 cut(s) 122
Psp5II RGGWCCY 2 cut(s) 1068, 1345
Psp6I CCWGG 4 cut(s) 991, 1081, 1218, 1702
PspCI CACGTG 1 cut(s) 1570
PspEI GGTNACC 1 cut(s) 284
PspFI CCCAGC 1 cut(s) 494
PspGI CCWGG 4 cut(s) 991, 1081, 1218, 1702
PspN4I GGNNCC 9 cut(s) 90, 786, 933, 970, 1069, 1070, 1164, 1224, 1450
PspPI GGNCC 5 cut(s) 89, 1068, 1154, 1223, 1345
PspPPI RGGWCCY 2 cut(s) 1068, 1345
PstI CTGCAG 2 cut(s) 140, 1615
PsuI RGATCY 6 cut(s) 369, 440, 482, 784, 968, 1286
PvuI CGATCG 1 cut(s) 295
PvuII CAGCTG 1 cut(s) 1610
RsaI GTAC 5 cut(s) 738, 855, 1164, 1777, 1803
RsaNI GTAC 5 cut(s) 737, 854, 1163, 1776, 1802
RseI CAYNNNNRTG 2 cut(s) 279, 1302
SaqAI TTAA 9 cut(s) 132, 171, 258, 648, 842, 888, 1059, 1184, 1454
SatI GCNGC 5 cut(s) 139, 492, 1473, 1611, 1721
Sau3AI GATC 8 cut(s) 292, 369, 440, 482, 784, 968, 1286, 1486
Sau96I GGNCC 5 cut(s) 89, 1068, 1154, 1223, 1345
SchI GAGTC 1 cut(s) 604
ScrFI CCNGG 4 cut(s) 993, 1083, 1220, 1704
SduI GDGCHC 3 cut(s) 217, 430, 1711
SfaNI GCATC 8 cut(s) 56, 78, 259, 649, 818, 920, 1303, 1697
SfcI CTRYAG 2 cut(s) 136, 1611
SinI GGWCC 5 cut(s) 89, 1068, 1154, 1223, 1345
SmiMI CAYNNNNRTG 2 cut(s) 279, 1302
SmlI CTYRAG 1 cut(s) 703
SmoI CTYRAG 1 cut(s) 703
SphI GCATGC 1 cut(s) 1420
Sse9I AATT 9 cut(s) 73, 155, 249, 828, 843, 892, 903, 943, 1526
SsiI CCGC 3 cut(s) 141, 1053, 1659
SspI AATATT 3 cut(s) 304, 760, 1424
SspMI CTAG 5 cut(s) 444, 486, 579, 834, 1320
StyD4I CCNGG 4 cut(s) 991, 1081, 1218, 1702
StyI CCWWGG 1 cut(s) 572
TaaI ACNGT 3 cut(s) 278, 356, 1097
TaiI ACGT 3 cut(s) 667, 1572, 1793
TaqI TCGA 2 cut(s) 477, 618
TasI AATT 9 cut(s) 73, 155, 249, 828, 843, 892, 903, 943, 1526
TatI WGTACW 1 cut(s) 1801
TfiI GAWTC 5 cut(s) 144, 512, 671, 1595, 1616
Tru1I TTAA 9 cut(s) 132, 171, 258, 648, 842, 888, 1059, 1184, 1454
Tru9I TTAA 9 cut(s) 132, 171, 258, 648, 842, 888, 1059, 1184, 1454
TscAI CASTG 8 cut(s) 367, 430, 795, 931, 1102, 1164, 1260, 1360
TseFI GTSAC 3 cut(s) 362, 391, 1641
TseI GCWGC 5 cut(s) 138, 491, 1472, 1610, 1720
Tsp45I GTSAC 3 cut(s) 362, 391, 1641
TspDTI ATGAA 7 cut(s) 17, 313, 891, 1023, 1376, 1640, 1802
TspGWI ACGGA 1 cut(s) 1812
TspRI CASTG 8 cut(s) 367, 430, 795, 931, 1102, 1164, 1260, 1360
VneI GTGCAC 3 cut(s) 213, 426, 1707
VpaK11BI GGWCC 5 cut(s) 89, 1068, 1154, 1223, 1345
XapI RAATTY 5 cut(s) 155, 249, 828, 892, 903
XbaI TCTAGA 2 cut(s) 443, 578
XceI RCATGY 5 cut(s) 434, 560, 721, 1136, 1420
XcmI CCANNNNNNNNNTGG 1 cut(s) 1700
XspI CTAG 5 cut(s) 444, 486, 579, 834, 1320
Zsp2I ATGCAT 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.