MD15G1114500.v1.1

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006494
Physical Location & Seq
Reverse (-)
8074616 .. 8079941
5326 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1114500.v1.1.491

Sequence Viewer

Length: 2211 bp
ATGGGAAAAGGGTCGAAGGAAGATGAGAAAATCGAAAGAACAATTCGCAGTCTTTTGAAACTTCCCGAGAATAAAAGATGCATCAACTGCAATAGCTTGGGACCACAATATGTTTGCACGACCTTCATGACATTCGTCTGTACAAATTGTAGTGGAGTACATCGGGAGTTCACTCACCGAGTAAAATCAGTGTCAATGGCCAAATTTAATGCAGAAGAAGTTAGTGCACTTCAAGCAGGGGGAAACGAGAGAGTTAGACAAATTTATTTTAAAGATTGGGATCCTCAGTACCATTCTTACCCTGATGCCAGTAATCTTCATGGACTCAGGAGTTTTATCAAGCATGTATACATTGATAGAAAATACACTGGCGAGAGGAGTGTCTACACCGGCGCGAGGAGTATCAACACTAGTGAGAGGAGTGGCAACAAGCTCCCAAGGCTGCAATTGAGCATTGAGGGGTCTGATGATAATCGGAGGATTGGTGGACATCATACTGGATCTAGGAGCTTCCATGATGATAACAGGTTGGAACGGGGTTATAGTTATAGTCCAAGTGGAAGAAATTTAAGATATTATTATGATGAAAGAAGAAGTCCTCGATATTCTCCAGAAAATTCAAGAAGTGGTGGTTTTAAGAAAGTTCCCCTACGTTTTGAGATAGTTGATAATAGGATTCGGGCAGAAAAACGAGGATCCTCAAGTGGAGAATACAAGGTCCAAAGCAGGACACCAGACAATAAGAAGACCGCAGAAAGCTCCCGTTCTCCCGTTGCGCCCCCTGTTAAAGATAAACTAGGGGAAAATGTGCCTTCTCCGCAGGTTGGTGAAAGGTCCACAGCAAATCAGAACGATACTGATGGTTCTGCTCATAATCAGAAAACCAAATCTACCGGTGGCCATGTTGGGAATGCGGCGGAACAAAAAGTAGTAAGCTTGTTTGATTTCAATACTGATTCTGAGCCACCACATGCTGCAGAATCGCAATTGGAAGAAACACCTCCACCCAATAACGATAACAACTGGGCCTCGTTTGAATCATCTACAACGGAGAAGGCACCTCAGGTTCCAAATGCAGACCCTCTGGAGTCTTTGCTATTTGAATTGTCAAGTCCCTCATCTGTTCCTGTGAGTAATGCATCTGAGACACCACGCAATGATGGTGCTCCCTCAACTGCGGGCATAAACACCATGCCTCCCGGTGGTGCTTCCCCAGCTGCACCTGCAGAGCAGATGCCAACATTACTTAATACAAGTAATGCATCTACATCTACATCGACTAGTACCACTGTAGCGGCACAACCTGCTGATGCAGGTCCTGTGCAAGATCTTGCTATTTTTGGTGGCGACACCACTGTAAAAGCTACTGATGAGAAGCAGTTAGTACTAAGTACACCGCAAGATCAGTCCTCTATATCCTTTGCCGCGGATAGTGGTTCTACTTCACAACATGTCAATTATGGAGTTGGAGCTTCAAACGGTGAGTTAAGGAATTTGTCACTGGAACCAAGCATACAAAGATCTTTCAGTATTCCTGCTGAAAAATCATCTCAACCTATGTTGAATCCAGCTGAACACACCGACAGTGGCAGTGGAGCTAGATCCCAAGTGGAAATGAATTCTAGTATGCGAAAGGAACTCCCAGCGGACCTTTTTACTGCAAGTTACTCATCAGCCCCAGGACAAGCTTCAGGCTGGCATACTGCTCCAGCTCATGGAATGGGGTACAGCATGCAGTATTACCCTACCGTGATGCCAGCTTCAGTATTTCCGGGTCCAGCAAAACCCAAAAACCCTTTTGATCTTGATGAAGAAAAAAGTCTAGTAACTTCCACACATTTTCCTTCCATGTCTTCATTGCAAGGAGCTCTGCATAACGTGCCAGTACCAGGCTTGATGCATGCTTCGAGCCTTCCTACTATGTCTCAGTTTAAGATACCCCAGTCTCCAACTAATGAATCCATGATGATGCCTTCACACTCGCCTTCCTTCACAAATGCTTTCTCTCCGAGGGCATACTTGGGGCATCAATTACCTAATAATGTGCAATTTTTAAGACCGCAAGGAGTTGGCGGCTTTGGCAGGGACGAAGCTGCTTTTGGATCCCTAACCACAACTCAGCAACCGTTTCAGCAACCAAGCGTCAAATACCCAGCACCCAGCAGCAGCTCAGAAACTTTTTCTTCAATGAGAGGAAACCCATTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArfGap

Protein Analysis

737

Amino Acids

79.74

Weight (kDa)

6.87

Isoelectric Point (pI)

61.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArfGap PF01412 13 - 123 1.6e-28 Putative GTPase activating protein for Arf
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1231
Acc36I ACCTGC 4 cut(s) 811, 1231, 1304, 1312
AccB1I GGYRCC 1 cut(s) 1057
AccB7I CCANNNNNTGG 1 cut(s) 1715
AccI GTMKAC 2 cut(s) 348, 384
AccII CGCG 2 cut(s) 395, 1427
AclWI GGATC 8 cut(s) 275, 288, 508, 690, 703, 1596, 2097, 2110
AcoI YGGCCR 2 cut(s) 198, 898
AcsI RAATTY 6 cut(s) 203, 261, 565, 616, 1492, 1618
AcuI CTGAAG 2 cut(s) 1674, 1746
AfaI GTAC 8 cut(s) 142, 159, 290, 1285, 1386, 1393, 1727, 1887
AfiI CCNNNNNNNGG 5 cut(s) 396, 824, 1202, 1715, 1889
AflIII ACRYGT 1 cut(s) 1450
AgeI ACCGGT 1 cut(s) 893
AgsI TTSAA 9 cut(s) 58, 233, 621, 949, 1037, 1103, 1476, 1564, 2187
AhlI ACTAGT 2 cut(s) 410, 1280
AjnI CCWGG 2 cut(s) 1678, 1888
AjuI GAANNNNNNNTTGG 2 cut(s) 2082, 2114
Alw21I GWGCWC 3 cut(s) 229, 1168, 1870
Alw26I GTCTC 3 cut(s) 1139, 1929, 1950
Alw44I GTGCAC 1 cut(s) 225
AlwI GGATC 8 cut(s) 275, 288, 508, 690, 703, 1596, 2097, 2110
AlwNI CAGNNNCTG 1 cut(s) 1319
Ama87I CYCGRG 1 cut(s) 65
AoxI GGCC 3 cut(s) 198, 898, 1026
ApaLI GTGCAC 1 cut(s) 225
ApeKI GCWGC 6 cut(s) 442, 974, 1217, 2093, 2163, 2166
ApoI RAATTY 6 cut(s) 203, 261, 565, 616, 1492, 1618
AsiGI ACCGGT 1 cut(s) 893
AspLEI GCGC 2 cut(s) 395, 778
AspS9I GGNCC 7 cut(s) 101, 718, 834, 1026, 1316, 1648, 1775
AsuC2I CCSGG 2 cut(s) 1200, 1773
AsuHPI GGTGA 3 cut(s) 167, 839, 1493
AvaI CYCGRG 1 cut(s) 65
AvaII GGWCC 6 cut(s) 101, 718, 834, 1316, 1648, 1775
AxyI CCTNAGG 1 cut(s) 1062
BaeGI GKGCMC 1 cut(s) 229
BaeI ACNNNNGTAYC 2 cut(s) 1928, 1961
BalI TGGCCA 2 cut(s) 200, 900
BamHI GGATCC 3 cut(s) 280, 695, 2102
BanI GGYRCC 1 cut(s) 1057
BanII GRGCYC 1 cut(s) 1870
BbsI GAAGAC 2 cut(s) 752, 1845
Bbv12I GWGCWC 3 cut(s) 229, 1168, 1870
BbvI GCAGC 6 cut(s) 429, 961, 1204, 2080, 2175, 2178
BccI CCATC 2 cut(s) 854, 1154
BciT130I CCWGG 2 cut(s) 1680, 1890
BcnI CCSGG 2 cut(s) 1200, 1773
BcoDI GTCTC 3 cut(s) 1139, 1929, 1950
BcuI ACTAGT 2 cut(s) 410, 1280
BfaI CTAG 7 cut(s) 411, 504, 797, 1281, 1599, 1623, 1823
BfmI CTRYAG 3 cut(s) 975, 1224, 1290
BfuAI ACCTGC 4 cut(s) 811, 1231, 1304, 1312
BglII AGATCT 2 cut(s) 1327, 1520
BmcAI AGTACT 1 cut(s) 1386
Bme1390I CCNGG 4 cut(s) 1200, 1680, 1773, 1890
Bme18I GGWCC 6 cut(s) 101, 718, 834, 1316, 1648, 1775
BmeT110I CYCGRG 1 cut(s) 65
BmgT120I GGNCC 7 cut(s) 101, 718, 834, 1026, 1316, 1648, 1775
BmiI GGNNCC 8 cut(s) 102, 282, 697, 1059, 1068, 1506, 1776, 2104
BmrFI CCNGG 4 cut(s) 1200, 1680, 1773, 1890
BmrI ACTGGG 2 cut(s) 1033, 1936
BmuI ACTGGG 2 cut(s) 1033, 1936
BoxI GACNNNNGTC 1 cut(s) 134
BpiI GAAGAC 2 cut(s) 752, 1845
BpmI CTGGAG 3 cut(s) 594, 1106, 1692
BpuEI CTTGAG 1 cut(s) 685
BpuMI CCSGG 2 cut(s) 1200, 1773
BsaBI GATNNNNATC 2 cut(s) 279, 471
BsaJI CCNNGG 4 cut(s) 437, 1425, 1678, 2009
BsaWI WCCGGW 1 cut(s) 893
BsaXI ACNNNNNCTCC 4 cut(s) 469, 499, 1180, 1210
Bsc4I CCNNNNNNNGG 5 cut(s) 396, 824, 1202, 1715, 1889
Bse118I RCCGGY 2 cut(s) 389, 893
Bse1I ACTGG 7 cut(s) 309, 373, 502, 1028, 1506, 1883, 1942
Bse21I CCTNAGG 1 cut(s) 1062
Bse3DI GCAATG 2 cut(s) 1162, 1856
Bse8I GATNNNNATC 2 cut(s) 279, 471
BseBI CCWGG 2 cut(s) 1680, 1890
BseDI CCNNGG 4 cut(s) 437, 1425, 1678, 2009
BseJI GATNNNNATC 2 cut(s) 279, 471
BseLI CCNNNNNNNGG 5 cut(s) 396, 824, 1202, 1715, 1889
BseMI GCAATG 2 cut(s) 1162, 1856
BseMII CTCAG 8 cut(s) 299, 340, 951, 1076, 1134, 1940, 2132, 2184
BseNI ACTGG 7 cut(s) 309, 373, 502, 1028, 1506, 1883, 1942
BseRI GAGGAG 3 cut(s) 391, 412, 433
BseSI GKGCMC 1 cut(s) 229
BseXI GCAGC 6 cut(s) 429, 961, 1204, 2080, 2175, 2178
BseYI CCCAGC 4 cut(s) 1213, 1642, 2152, 2159
BsgI GTGCAG 1 cut(s) 1203
Bsh1236I CGCG 2 cut(s) 395, 1427
BshFI GGCC 3 cut(s) 200, 900, 1028
BshNI GGYRCC 1 cut(s) 1057
BshTI ACCGGT 1 cut(s) 893
BsiHKAI GWGCWC 3 cut(s) 229, 1168, 1870
BsiHKCI CYCGRG 1 cut(s) 65
BsiSI CCGG 4 cut(s) 390, 894, 1200, 1772
BslFI GGGAC 3 cut(s) 114, 1098, 2099
BslI CCNNNNNNNGG 5 cut(s) 396, 824, 1202, 1715, 1889
BsmAI GTCTC 3 cut(s) 1139, 1929, 1950
BsmFI GGGAC 3 cut(s) 114, 1098, 2099
BsmI GAATGC 1 cut(s) 916
BsnI GGCC 3 cut(s) 200, 900, 1028
BsoBI CYCGRG 1 cut(s) 65
Bsp1286I GDGCHC 3 cut(s) 229, 1168, 1870
Bsp1407I TGTACA 1 cut(s) 140
Bsp143I GATC 9 cut(s) 280, 500, 695, 1327, 1402, 1520, 1601, 1801, 2102
BspANI GGCC 3 cut(s) 200, 900, 1028
BspCNI CTCAG 8 cut(s) 298, 339, 952, 1075, 1135, 1939, 2131, 2183
BspFNI CGCG 2 cut(s) 395, 1427
BspHI TCATGA 1 cut(s) 126
BspLI GGNNCC 8 cut(s) 102, 282, 697, 1059, 1068, 1506, 1776, 2104
BspMAI CTGCAG 2 cut(s) 979, 1228
BspMI ACCTGC 4 cut(s) 811, 1231, 1304, 1312
BspPI GGATC 8 cut(s) 275, 288, 508, 690, 703, 1596, 2097, 2110
BspT107I GGYRCC 1 cut(s) 1057
BsrDI GCAATG 2 cut(s) 1162, 1856
BsrFI RCCGGY 2 cut(s) 389, 893
BsrGI TGTACA 1 cut(s) 140
BsrI ACTGG 7 cut(s) 309, 373, 502, 1028, 1506, 1883, 1942
BssAI RCCGGY 2 cut(s) 389, 893
BssECI CCNNGG 4 cut(s) 437, 1425, 1678, 2009
BssMI GATC 9 cut(s) 280, 500, 695, 1327, 1402, 1520, 1601, 1801, 2102
BssNAI GTATAC 1 cut(s) 349
BssT1I CCWWGG 1 cut(s) 437
Bst1107I GTATAC 1 cut(s) 349
Bst2UI CCWGG 2 cut(s) 1680, 1890
Bst4CI ACNGT 6 cut(s) 1291, 1357, 1481, 1586, 1750, 2127
BstAPI GCANNNNNTGC 3 cut(s) 87, 1304, 1879
BstAUI TGTACA 1 cut(s) 140
BstC8I GCNNGC 5 cut(s) 1180, 1697, 1733, 1758, 1902
BstDEI CTNAG 9 cut(s) 285, 326, 960, 1062, 1143, 1388, 1926, 2118, 2170
BstDSI CCRYGG 1 cut(s) 1425
BstFNI CGCG 2 cut(s) 395, 1427
BstHHI GCGC 2 cut(s) 395, 778
BstKTI GATC 9 cut(s) 283, 503, 698, 1330, 1405, 1523, 1604, 1804, 2105
BstMAI GTCTC 3 cut(s) 1139, 1929, 1950
BstMBI GATC 9 cut(s) 280, 500, 695, 1327, 1402, 1520, 1601, 1801, 2102
BstMWI GCNNNNNNNGC 9 cut(s) 87, 233, 439, 817, 1214, 1223, 1304, 1879, 2079
BstNI CCWGG 2 cut(s) 1680, 1890
BstNSI RCATGY 5 cut(s) 347, 974, 1454, 1735, 1904
BstPAI GACNNNNGTC 1 cut(s) 134
BstSCI CCNGG 4 cut(s) 1198, 1678, 1771, 1888
BstSFI CTRYAG 3 cut(s) 975, 1224, 1290
BstSLI GKGCMC 1 cut(s) 229
BstUI CGCG 2 cut(s) 395, 1427
BstV1I GCAGC 6 cut(s) 429, 961, 1204, 2080, 2175, 2178
BstV2I GAAGAC 2 cut(s) 752, 1845
BstX2I RGATCY 7 cut(s) 280, 500, 695, 1327, 1520, 1601, 2102
BstYI RGATCY 7 cut(s) 280, 500, 695, 1327, 1520, 1601, 2102
BstZ17I GTATAC 1 cut(s) 349
Bsu36I CCTNAGG 1 cut(s) 1062
BsuRI GGCC 3 cut(s) 200, 900, 1028
BtgI CCRYGG 1 cut(s) 1425
BtsI GCAGTG 1 cut(s) 1597
BtsIMutI CAGTG 7 cut(s) 195, 366, 1287, 1353, 1499, 1591, 1597
BveI ACCTGC 4 cut(s) 811, 1231, 1304, 1312
Cac8I GCNNGC 5 cut(s) 1180, 1697, 1733, 1758, 1902
CaiI CAGNNNCTG 1 cut(s) 1319
CciI TCATGA 1 cut(s) 126
CfoI GCGC 2 cut(s) 395, 778
Cfr10I RCCGGY 2 cut(s) 389, 893
Cfr13I GGNCC 7 cut(s) 101, 718, 834, 1026, 1316, 1648, 1775
Cfr42I CCGCGG 1 cut(s) 1428
CseI GACGC 1 cut(s) 2131
Csp6I GTAC 8 cut(s) 141, 158, 289, 1284, 1385, 1392, 1726, 1886
CspAI ACCGGT 1 cut(s) 893
CviQI GTAC 8 cut(s) 141, 158, 289, 1284, 1385, 1392, 1726, 1886
DdeI CTNAG 9 cut(s) 285, 326, 960, 1062, 1143, 1388, 1926, 2118, 2170
DpnI GATC 9 cut(s) 282, 502, 697, 1329, 1404, 1522, 1603, 1803, 2104
DpnII GATC 9 cut(s) 280, 500, 695, 1327, 1402, 1520, 1601, 1801, 2102
DraI TTTAAA 1 cut(s) 271
EaeI YGGCCR 2 cut(s) 198, 898
EciI GGCGGA 1 cut(s) 932
Ecl136II GAGCTC 1 cut(s) 1868
Eco130I CCWWGG 1 cut(s) 437
Eco24I GRGCYC 1 cut(s) 1870
Eco47I GGWCC 6 cut(s) 101, 718, 834, 1316, 1648, 1775
Eco53kI GAGCTC 1 cut(s) 1868
Eco57I CTGAAG 2 cut(s) 1674, 1746
Eco81I CCTNAGG 1 cut(s) 1062
Eco88I CYCGRG 1 cut(s) 65
EcoICRI GAGCTC 1 cut(s) 1868
EcoO109I RGGNCCY 1 cut(s) 1316
EcoRI GAATTC 1 cut(s) 1618
EcoRII CCWGG 2 cut(s) 1678, 1888
EcoT14I CCWWGG 1 cut(s) 437
EcoT22I ATGCAT 4 cut(s) 83, 1141, 1264, 1902
EcoT38I GRGCYC 1 cut(s) 1870
ErhI CCWWGG 1 cut(s) 437
FaqI GGGAC 3 cut(s) 114, 1098, 2099
FauI CCCGC 1 cut(s) 1171
FblI GTMKAC 2 cut(s) 348, 384
FriOI GRGCYC 1 cut(s) 1870
FspBI CTAG 7 cut(s) 411, 504, 797, 1281, 1599, 1623, 1823
GlaI GCGC 2 cut(s) 394, 777
GsaI CCCAGC 4 cut(s) 1217, 1646, 2156, 2163
GsuI CTGGAG 3 cut(s) 594, 1106, 1692
HaeIII GGCC 3 cut(s) 200, 900, 1028
HapII CCGG 4 cut(s) 390, 894, 1200, 1772
HgaI GACGC 1 cut(s) 2131
HhaI GCGC 2 cut(s) 395, 778
Hin6I GCGC 2 cut(s) 393, 776
HinP1I GCGC 2 cut(s) 393, 776
HindIII AAGCTT 2 cut(s) 934, 1686
HinfI GANTC 8 cut(s) 324, 676, 956, 980, 1037, 1088, 1564, 1958
HpaII CCGG 4 cut(s) 390, 894, 1200, 1772
HphI GGTGA 3 cut(s) 167, 839, 1493
Hpy166II GTNNAC 7 cut(s) 171, 227, 349, 385, 488, 837, 1394
Hpy188I TCNGA 8 cut(s) 466, 477, 849, 879, 961, 1144, 2010, 2173
Hpy188III TCNNGA 8 cut(s) 65, 127, 164, 328, 611, 621, 1085, 1805
Hpy8I GTNNAC 7 cut(s) 171, 227, 349, 385, 488, 837, 1394
HpyAV CCTTC 9 cut(s) 10, 133, 822, 1048, 1854, 1922, 1983, 1995, 1999
HpyCH4III ACNGT 6 cut(s) 1291, 1357, 1481, 1586, 1750, 2127
HpyCH4IV ACGT 2 cut(s) 652, 1878
HpyF10VI GCNNNNNNNGC 9 cut(s) 87, 233, 439, 817, 1214, 1223, 1304, 1879, 2079
HpyF3I CTNAG 9 cut(s) 285, 326, 960, 1062, 1143, 1388, 1926, 2118, 2170
HpySE526I ACGT 2 cut(s) 652, 1878
HspAI GCGC 2 cut(s) 393, 776
KspI CCGCGG 1 cut(s) 1428
Kzo9I GATC 9 cut(s) 280, 500, 695, 1327, 1402, 1520, 1601, 1801, 2102
LmnI GCTCC 8 cut(s) 438, 507, 764, 1171, 1469, 1595, 1711, 1865
Lsp1109I GCAGC 6 cut(s) 429, 961, 1204, 2080, 2175, 2178
MaeI CTAG 7 cut(s) 411, 504, 797, 1281, 1599, 1623, 1823
MaeII ACGT 2 cut(s) 652, 1878
MaeIII GTNAC 3 cut(s) 1497, 1664, 1825
MalI GATC 9 cut(s) 282, 502, 697, 1329, 1404, 1522, 1603, 1803, 2104
MboI GATC 9 cut(s) 280, 500, 695, 1327, 1402, 1520, 1601, 1801, 2102
MfeI CAATTG 2 cut(s) 446, 986
MflI RGATCY 7 cut(s) 280, 500, 695, 1327, 1520, 1601, 2102
MhlI GDGCHC 3 cut(s) 229, 1168, 1870
MlsI TGGCCA 2 cut(s) 200, 900
MluNI TGGCCA 2 cut(s) 200, 900
MlyI GAGTC 2 cut(s) 318, 1097
MmeI TCCRAC 3 cut(s) 510, 1447, 1973
Mox20I TGGCCA 2 cut(s) 200, 900
Mph1103I ATGCAT 4 cut(s) 83, 1141, 1264, 1902
MscI TGGCCA 2 cut(s) 200, 900
MseI TTAA 9 cut(s) 207, 270, 569, 636, 786, 1248, 1487, 1932, 2054
MslI CAYNNNNRTG 2 cut(s) 1967, 2206
Msp20I TGGCCA 2 cut(s) 200, 900
MspA1I CMGCKG 4 cut(s) 1217, 1427, 1571, 1646
MspI CCGG 4 cut(s) 390, 894, 1200, 1772
MspR9I CCNGG 4 cut(s) 1200, 1680, 1773, 1890
MunI CAATTG 2 cut(s) 446, 986
Mva1269I GAATGC 1 cut(s) 916
MvaI CCWGG 2 cut(s) 1680, 1890
MvnI CGCG 2 cut(s) 395, 1427
MwoI GCNNNNNNNGC 9 cut(s) 87, 233, 439, 817, 1214, 1223, 1304, 1879, 2079
NciI CCSGG 2 cut(s) 1200, 1773
NdeII GATC 9 cut(s) 280, 500, 695, 1327, 1402, 1520, 1601, 1801, 2102
NlaIV GGNNCC 8 cut(s) 102, 282, 697, 1059, 1068, 1506, 1776, 2104
NmuCI GTSAC 1 cut(s) 1497
NsiI ATGCAT 4 cut(s) 83, 1141, 1264, 1902
NspI RCATGY 5 cut(s) 347, 974, 1454, 1735, 1904
PaeI GCATGC 2 cut(s) 1735, 1904
PagI TCATGA 1 cut(s) 126
PaqCI CACCTGC 1 cut(s) 1231
PciI ACATGT 1 cut(s) 1450
PctI GAATGC 1 cut(s) 916
PfeI GAWTC 6 cut(s) 676, 956, 980, 1037, 1564, 1958
PflMI CCANNNNNTGG 1 cut(s) 1715
PinAI ACCGGT 1 cut(s) 893
PleI GAGTC 2 cut(s) 318, 1096
PpsI GAGTC 2 cut(s) 318, 1096
PpuMI RGGWCCY 1 cut(s) 1316
PscI ACATGT 1 cut(s) 1450
PshAI GACNNNNGTC 1 cut(s) 134
Psp124BI GAGCTC 1 cut(s) 1870
Psp5II RGGWCCY 1 cut(s) 1316
Psp6I CCWGG 2 cut(s) 1678, 1888
PspFI CCCAGC 4 cut(s) 1213, 1642, 2152, 2159
PspGI CCWGG 2 cut(s) 1678, 1888
PspN4I GGNNCC 8 cut(s) 102, 282, 697, 1059, 1068, 1506, 1776, 2104
PspPI GGNCC 7 cut(s) 101, 718, 834, 1026, 1316, 1648, 1775
PspPPI RGGWCCY 1 cut(s) 1316
PsrI GAACNNNNNNTAC 2 cut(s) 1497, 1529
PstI CTGCAG 2 cut(s) 979, 1228
PstNI CAGNNNCTG 1 cut(s) 1319
PsuI RGATCY 7 cut(s) 280, 500, 695, 1327, 1520, 1601, 2102
PvuII CAGCTG 2 cut(s) 1217, 1571
RsaI GTAC 8 cut(s) 142, 159, 290, 1285, 1386, 1393, 1727, 1887
RsaNI GTAC 8 cut(s) 141, 158, 289, 1284, 1385, 1392, 1726, 1886
RseI CAYNNNNRTG 2 cut(s) 1967, 2206
SacI GAGCTC 1 cut(s) 1870
SacII CCGCGG 1 cut(s) 1428
SaqAI TTAA 9 cut(s) 207, 270, 569, 636, 786, 1248, 1487, 1932, 2054
Sau3AI GATC 9 cut(s) 280, 500, 695, 1327, 1402, 1520, 1601, 1801, 2102
Sau96I GGNCC 7 cut(s) 101, 718, 834, 1026, 1316, 1648, 1775
ScaI AGTACT 1 cut(s) 1386
SchI GAGTC 2 cut(s) 318, 1097
ScrFI CCNGG 4 cut(s) 1200, 1680, 1773, 1890
SduI GDGCHC 3 cut(s) 229, 1168, 1870
SfcI CTRYAG 3 cut(s) 975, 1224, 1290
Sfr303I CCGCGG 1 cut(s) 1428
SgrAI CRCCGGYG 1 cut(s) 389
SgrBI CCGCGG 1 cut(s) 1428
SinI GGWCC 6 cut(s) 101, 718, 834, 1316, 1648, 1775
SmiMI CAYNNNNRTG 2 cut(s) 1967, 2206
SmlI CTYRAG 1 cut(s) 700
SmoI CTYRAG 1 cut(s) 700
SpeI ACTAGT 2 cut(s) 410, 1280
SphI GCATGC 2 cut(s) 1735, 1904
SspMI CTAG 7 cut(s) 411, 504, 797, 1281, 1599, 1623, 1823
SstI GAGCTC 1 cut(s) 1870
StyD4I CCNGG 4 cut(s) 1198, 1678, 1771, 1888
StyI CCWWGG 1 cut(s) 437
TaaI ACNGT 6 cut(s) 1291, 1357, 1481, 1586, 1750, 2127
TaiI ACGT 2 cut(s) 655, 1881
TaqI TCGA 5 cut(s) 14, 33, 601, 1277, 1907
TatI WGTACW 4 cut(s) 140, 157, 1384, 1391
TauI GCSGC 4 cut(s) 917, 1298, 1427, 2076
TfiI GAWTC 6 cut(s) 676, 956, 980, 1037, 1564, 1958
Tru1I TTAA 9 cut(s) 207, 270, 569, 636, 786, 1248, 1487, 1932, 2054
Tru9I TTAA 9 cut(s) 207, 270, 569, 636, 786, 1248, 1487, 1932, 2054
TscAI CASTG 7 cut(s) 195, 373, 1294, 1360, 1506, 1591, 1597
TseFI GTSAC 1 cut(s) 1497
TseI GCWGC 6 cut(s) 442, 974, 1217, 2093, 2163, 2166
Tsp45I GTSAC 1 cut(s) 1497
TspDTI ATGAA 7 cut(s) 115, 308, 600, 1631, 1824, 1845, 1971
TspGWI ACGGA 1 cut(s) 1064
TspRI CASTG 7 cut(s) 195, 373, 1294, 1360, 1506, 1591, 1597
Van91I CCANNNNNTGG 1 cut(s) 1715
VneI GTGCAC 1 cut(s) 225
VpaK11BI GGWCC 6 cut(s) 101, 718, 834, 1316, 1648, 1775
XapI RAATTY 6 cut(s) 203, 261, 565, 616, 1492, 1618
XceI RCATGY 5 cut(s) 347, 974, 1454, 1735, 1904
XmiI GTMKAC 2 cut(s) 348, 384
XspI CTAG 7 cut(s) 411, 504, 797, 1281, 1599, 1623, 1823
ZrmI AGTACT 1 cut(s) 1386
Zsp2I ATGCAT 4 cut(s) 83, 1141, 1264, 1902
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.