Rh4DG050300

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0006494
Physical Location & Seq
Reverse (-)
8050864 .. 8078194
27331 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG050300.1

Sequence Viewer

Length: 513 bp
ATGCAGGGACCGCAATATGTTTGCACGACCTTCTTGACATTTGTTTGTACAAATTGTAGTGGAGTGCATCGGGAGTTCACTCACCGAGTAAAATCAGTGTCCATGGCTAAATTCACTGCAGAAGAAGTAAATTCTCTCCAGGCAGGGGGAAATGAGCGAGCAAGACAAATTTATTTCAAAGAATTTGATCCTCAGTACCATTCTTTTCCTGATGGCAGGATAGTGATGGTTCAATACAAAGACTTTCTTGCCTTGAGAAGAAAGCAAGAATTAAAGTTTTTAGAGCATATATATCTCCACTCCTTTTCAAATCTATCCTCAAATTTGTATGGGGAAAAAATGACAAAAATAGAGAGAGCTACTCCAAAAAAACAGACCATCCACCCAGCAAACCGTAAACAGCAATTTGAGTACCCAAGACGACAAAGTTTTGATAAACGTCCAGCCACCCCAGTGCAGCCCCCAAAATCGCCGATCAATCCGGCACATTACAACCCTTCCCCAATACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArfGap

Protein Analysis

170

Amino Acids

19.82

Weight (kDa)

9.74

Isoelectric Point (pI)

62.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArfGap PF01412 2 - 71 2.7e-18 Putative GTPase activating protein for Arf
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 11
AclWI GGATC 1 cut(s) 182
AcsI RAATTY 5 cut(s) 110, 130, 168, 182, 322
AfaI GTAC 3 cut(s) 49, 197, 413
AfiI CCNNNNNNNGG 1 cut(s) 145
AgsI TTSAA 3 cut(s) 178, 233, 309
AjnI CCWGG 1 cut(s) 138
AleI CACNNNNGTG 1 cut(s) 452
AluBI AGCT 1 cut(s) 359
AluI AGCT 1 cut(s) 359
AlwI GGATC 1 cut(s) 182
ApeKI GCWGC 1 cut(s) 457
ApoI RAATTY 5 cut(s) 110, 130, 168, 182, 322
AspS9I GGNCC 1 cut(s) 8
AsuHPI GGTGA 1 cut(s) 74
AvaII GGWCC 1 cut(s) 8
BbvI GCAGC 1 cut(s) 469
BccI CCATC 3 cut(s) 206, 220, 386
BciT130I CCWGG 1 cut(s) 140
BfmI CTRYAG 1 cut(s) 117
BisI GCNGC 1 cut(s) 458
BlsI GCNGC 1 cut(s) 459
Bme1390I CCNGG 1 cut(s) 140
Bme18I GGWCC 1 cut(s) 8
BmgT120I GGNCC 1 cut(s) 8
BmiI GGNNCC 1 cut(s) 9
BmrFI CCNGG 1 cut(s) 140
BmrI ACTGGG 1 cut(s) 446
BmsI GCATC 1 cut(s) 76
BmuI ACTGGG 1 cut(s) 446
BplI GAGNNNNNCTC 2 cut(s) 346, 378
BpmI CTGGAG 1 cut(s) 122
BpuEI CTTGAG 1 cut(s) 274
BsaJI CCNNGG 1 cut(s) 102
Bsc4I CCNNNNNNNGG 1 cut(s) 145
Bse1I ACTGG 1 cut(s) 452
BseBI CCWGG 1 cut(s) 140
BseDI CCNNGG 1 cut(s) 102
BseGI GGATG 1 cut(s) 378
BseLI CCNNNNNNNGG 1 cut(s) 145
BseMII CTCAG 1 cut(s) 206
BseNI ACTGG 1 cut(s) 452
BseXI GCAGC 1 cut(s) 469
BseYI CCCAGC 1 cut(s) 385
BsgI GTGCAG 1 cut(s) 476
BsiSI CCGG 1 cut(s) 482
BslFI GGGAC 1 cut(s) 21
BslI CCNNNNNNNGG 1 cut(s) 145
BsmFI GGGAC 1 cut(s) 21
Bsp1407I TGTACA 1 cut(s) 47
Bsp143I GATC 2 cut(s) 187, 474
Bsp19I CCATGG 1 cut(s) 102
BspACI CCGC 1 cut(s) 11
BspCNI CTCAG 1 cut(s) 205
BspLI GGNNCC 1 cut(s) 9
BspMAI CTGCAG 1 cut(s) 121
BspPI GGATC 1 cut(s) 182
BsrGI TGTACA 1 cut(s) 47
BsrI ACTGG 1 cut(s) 452
BssECI CCNNGG 1 cut(s) 102
BssMI GATC 2 cut(s) 187, 474
BssT1I CCWWGG 1 cut(s) 102
Bst2UI CCWGG 1 cut(s) 140
Bst4CI ACNGT 1 cut(s) 395
BstAUI TGTACA 1 cut(s) 47
BstC8I GCNNGC 1 cut(s) 159
BstDEI CTNAG 1 cut(s) 192
BstDSI CCRYGG 1 cut(s) 102
BstF5I GGATG 1 cut(s) 378
BstKTI GATC 2 cut(s) 190, 477
BstMBI GATC 2 cut(s) 187, 474
BstMWI GCNNNNNNNGC 1 cut(s) 10
BstNI CCWGG 1 cut(s) 140
BstSCI CCNGG 1 cut(s) 138
BstSFI CTRYAG 1 cut(s) 117
BstV1I GCAGC 1 cut(s) 469
BtgI CCRYGG 1 cut(s) 102
BtsCI GGATG 1 cut(s) 378
BtsI GCAGTG 1 cut(s) 114
BtsIMutI CAGTG 3 cut(s) 102, 114, 459
Cac8I GCNNGC 1 cut(s) 159
Cfr13I GGNCC 1 cut(s) 8
Csp6I GTAC 3 cut(s) 48, 196, 412
CviAII CATG 1 cut(s) 103
CviJI RGCY 4 cut(s) 107, 359, 446, 460
CviKI_1 RGCY 4 cut(s) 107, 359, 446, 460
CviQI GTAC 3 cut(s) 48, 196, 412
DdeI CTNAG 1 cut(s) 192
DpnI GATC 2 cut(s) 189, 476
DpnII GATC 2 cut(s) 187, 474
Eco130I CCWWGG 1 cut(s) 102
Eco47I GGWCC 1 cut(s) 8
EcoRII CCWGG 1 cut(s) 138
EcoT14I CCWWGG 1 cut(s) 102
ErhI CCWWGG 1 cut(s) 102
FaeI CATG 1 cut(s) 106
FaiI YATR 7 cut(s) 18, 104, 288, 290, 292, 330, 511
FalI AAGNNNNNCTT 2 cut(s) 231, 263
FaqI GGGAC 1 cut(s) 21
FatI CATG 1 cut(s) 102
Fnu4HI GCNGC 1 cut(s) 458
FokI GGATG 1 cut(s) 365
Fsp4HI GCNGC 1 cut(s) 458
GluI GCNGC 1 cut(s) 458
GsaI CCCAGC 1 cut(s) 389
GsuI CTGGAG 1 cut(s) 122
HapII CCGG 1 cut(s) 482
Hin1II CATG 1 cut(s) 106
HpaII CCGG 1 cut(s) 482
HphI GGTGA 1 cut(s) 74
Hpy166II GTNNAC 2 cut(s) 78, 398
Hpy188III TCNNGA 3 cut(s) 34, 71, 209
Hpy8I GTNNAC 2 cut(s) 78, 398
HpyAV CCTTC 2 cut(s) 40, 507
HpyCH4III ACNGT 1 cut(s) 395
HpyCH4IV ACGT 1 cut(s) 439
HpyCH4V TGCA 5 cut(s) 4, 24, 67, 119, 457
HpyF10VI GCNNNNNNNGC 1 cut(s) 10
HpyF3I CTNAG 1 cut(s) 192
HpySE526I ACGT 1 cut(s) 439
Hsp92II CATG 1 cut(s) 106
Kzo9I GATC 2 cut(s) 187, 474
LpnPI CCDG 9 cut(s) 125, 129, 152, 202, 222, 399, 456, 465, 495
Lsp1109I GCAGC 1 cut(s) 469
LweI GCATC 1 cut(s) 76
MaeII ACGT 1 cut(s) 439
MalI GATC 2 cut(s) 189, 476
MboI GATC 2 cut(s) 187, 474
MboII GAAGA 2 cut(s) 134, 270
MluCI AATT 8 cut(s) 52, 110, 130, 168, 182, 269, 322, 404
MnlI CCTC 2 cut(s) 201, 328
MseI TTAA 1 cut(s) 272
MslI CAYNNNNRTG 1 cut(s) 452
MspI CCGG 1 cut(s) 482
MspR9I CCNGG 1 cut(s) 140
MvaI CCWGG 1 cut(s) 140
MwoI GCNNNNNNNGC 1 cut(s) 10
NcoI CCATGG 1 cut(s) 102
NdeII GATC 2 cut(s) 187, 474
NlaIII CATG 1 cut(s) 106
NlaIV GGNNCC 1 cut(s) 9
OliI CACNNNNGTG 1 cut(s) 452
PkrI GCNGC 1 cut(s) 459
Psp6I CCWGG 1 cut(s) 138
PspFI CCCAGC 1 cut(s) 385
PspGI CCWGG 1 cut(s) 138
PspN4I GGNNCC 1 cut(s) 9
PspPI GGNCC 1 cut(s) 8
PstI CTGCAG 1 cut(s) 121
RsaI GTAC 3 cut(s) 49, 197, 413
RsaNI GTAC 3 cut(s) 48, 196, 412
RseI CAYNNNNRTG 1 cut(s) 452
SaqAI TTAA 1 cut(s) 272
SatI GCNGC 1 cut(s) 458
Sau3AI GATC 2 cut(s) 187, 474
Sau96I GGNCC 1 cut(s) 8
ScrFI CCNGG 1 cut(s) 140
SetI ASST 3 cut(s) 32, 361, 442
SfaNI GCATC 1 cut(s) 76
SfcI CTRYAG 1 cut(s) 117
SinI GGWCC 1 cut(s) 8
SmiMI CAYNNNNRTG 1 cut(s) 452
SmlI CTYRAG 1 cut(s) 253
SmoI CTYRAG 1 cut(s) 253
Sse9I AATT 8 cut(s) 52, 110, 130, 168, 182, 269, 322, 404
SsiI CCGC 1 cut(s) 11
StyD4I CCNGG 1 cut(s) 138
StyI CCWWGG 1 cut(s) 102
TaaI ACNGT 1 cut(s) 395
TaiI ACGT 1 cut(s) 442
TasI AATT 8 cut(s) 52, 110, 130, 168, 182, 269, 322, 404
TatI WGTACW 1 cut(s) 47
Tru1I TTAA 1 cut(s) 272
Tru9I TTAA 1 cut(s) 272
TscAI CASTG 3 cut(s) 102, 121, 459
TseI GCWGC 1 cut(s) 457
TspRI CASTG 3 cut(s) 102, 121, 459
VpaK11BI GGWCC 1 cut(s) 8
XapI RAATTY 5 cut(s) 110, 130, 168, 182, 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.