MD08G1135500.v1.1

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0006494
Physical Location & Seq
Reverse (-)
12857970 .. 12863227
5258 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1135500.v1.1.491

Sequence Viewer

Length: 2109 bp
ATGAGAAAAGGGTTGAAGGAAGATGAGAAAAGCGAAAGAACAATTCGCAGTCTTTTAAAACTTCCCGAGAATAAAAGATGCATCAACTGCAATAGCTTGGGACCGCAATATGTTTGCACGACCTTCTTGACATTTGTCTGTACAAAGTGTAGTGGAGTACATCGGGAGTTCACTCACCGAGTAAAATCAGTGTCAATGGCTAAATTTAATGCAGAAGAAGTTAGTGCACTTCAAGCAGGGGGAAATGAGCGAGCAAGACAAATTTATTTTAAAGAATGGGATCCTCAGTACCATTCTTACCCTGATGCCGGTAATCTTCATGGACTCAGGAGTTTTATAAAGCATGTATATGTTGATAGAAAATACACTGGCGAGAGGAGTGTCTACACTGGTGAGAGGAGTATCTACACTAGTGAGAGGAGTGGCAACAAGCTCCCAATGCTGCGATTGAGCACTGAGGAGTCTGATGAAAACCAGAAGGTTGGTGGACATCGTAGTGGATCCAGGAGCTTCCATGATGATGACAGGTTGGAACGGAGTAATAGTAATAGTCCAGGAGGAAGAAGTTTAAGATATTACTATAATGAAAGAAGAAGTCCTCGATATTCTCCAGAAAACTCAAGAAGTGGTGGTTTTATAAGACCTCCTCTACGTTTTGAGATTGTTGATAACAGGATTCGGGGGGAAAAACGTGGATCCTCAAGTGGAGAATCCAAGGTCCAAAGCAGGACACCAGACAATAAGAAGACCGCAGAAAGGTCCCATTCTCCTGTTGTGCCCCCTGTTAAAGATAAATTACGAGAAAACGTGCCTCCTCCACAGGTTGGTGAAAGTTCCACAGCAAATCAGAAGGATACTGATGGTTCTGCTCATAATCAGAAAACCAAATCTACTGGTGGTCAGGATGGGAATGCGACCGAACAAAATATAGTAAGCTTGATTGATTTCAATACTGATTCCGAGCCCCCACATGCTACAGAATCACAATTGCAGCAAACACCTCCACCCAATGAGGATAACAACTGGGCCTCATTTGGATCATCTACAACGGAGAAGGCACCTCAAGTTCCAAATGCAGACCCTCTGGAGTCTTTGCTGTTTGAATTGTCAACTCCCTCATGTGTTCCTGTGAGTAATGCATCTGAGACACCACATAATGATGGTGCTCCGTCAACTGCATCTATAAACACCATGCCTGCAGGTGGTCATTCCCCAGCTGATGCAGGTCCTGTGCAAGATCTTGCTATTTTTGGTGGTGACACCACTGTAAAAAGTGCTGATGAGAAGCAGTTAGTACTAAGTACACAGCAAGATCAGTCGTCTATATCCTTCACCGCAGATAGCGGTTCTACTTCACACGTCAATTTTGCAGTTGGAGCTTCAAACGCCGAGTTGAGGAACTTATCACTGGCACCAAGCATACAAAGATCTTTCAGTATTCCTGCTGAAAAATCATCTCAATCTATTTTGAATCCAGCTGAACAAACAGACAGTGGCAGTGGAGCTGGATCCCAAGTGGAAATGAATTCTAGCATACGAAAGGAACTCCCGGCAGACCTTTTTACTGCAAGTTACTCATCAGCCCCTGGACAAGCTTCAGGCTGGCATATTGCTCCAGCTCAGGGAATGGGGTACAACATGCAGTATTACCCTACCGCGACGCCGGCTCTAGCAATTCCGGGTCCAGCAAAACCAAAAAACCCGTTTGATCTTAATGAAGAAAAAAGCCTAGTAACTTCCACACATTTTCCTTCCATGTCTTCATTGCAAGGAGCTCTGACTAATGCTCCAGTACCAGGCTTGATGCATGCTTCGAGCCTTCCTACTATGTCGCAATTTGAGTCTAATGAATCCATGACGATGCCTTCGCACTCGCCTTCGTTCGCAAATGCTTTCTCTCCGAGGGCATACATGGGGCATCAATTACCTAATAATGTGCAATTTTTAAGACCGCAGGGAGTTGGCGGCTTTGGCAGGGACGAAGCTGCTTTTGGATCCCTAACCACAACTCAGCAATCATTTCAGCAACCAAGCGTCAGATACCCAGCACCCAGCAGCAGCAGCACCTTAGACACTTTTTCTCCGATGAGAGGAAACCCATTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArfGap

Protein Analysis

703

Amino Acids

76.19

Weight (kDa)

6.67

Isoelectric Point (pI)

63.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArfGap PF01412 13 - 123 2e-28 Putative GTPase activating protein for Arf
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 338, 638
AarI CACCTGC 1 cut(s) 1190
Acc36I ACCTGC 2 cut(s) 1190, 1214
AccB1I GGYRCC 2 cut(s) 1057, 1411
AccB7I CCANNNNNTGG 1 cut(s) 824
AccI GTMKAC 1 cut(s) 384
AccII CGCG 1 cut(s) 1658
AciI CCGC 7 cut(s) 104, 750, 1335, 1344, 1656, 1952, 1965
AcsI RAATTY 3 cut(s) 203, 261, 1525
AcuI CTGAAG 1 cut(s) 1581
AcyI GRCGYC 1 cut(s) 1661
AfaI GTAC 7 cut(s) 142, 159, 290, 1296, 1303, 1634, 1794
AfiI CCNNNNNNNGG 8 cut(s) 308, 756, 824, 1202, 1395, 1622, 1796, 2090
AflIII ACRYGT 1 cut(s) 1357
AgsI TTSAA 6 cut(s) 16, 233, 949, 1103, 1383, 1471
AhlI ACTAGT 1 cut(s) 410
AjiI CACGTC 1 cut(s) 1360
AjnI CCWGG 4 cut(s) 503, 553, 1585, 1795
AjuI GAANNNNNNNTTGG 2 cut(s) 1974, 2006
Alw21I GWGCWC 4 cut(s) 229, 455, 1168, 1777
Alw26I GTCTC 1 cut(s) 1139
Alw44I GTGCAC 1 cut(s) 225
AlwNI CAGNNNCTG 2 cut(s) 1229, 1586
Ama87I CYCGRG 1 cut(s) 65
AoxI GGCC 1 cut(s) 1026
ApaLI GTGCAC 1 cut(s) 225
ApeKI GCWGC 6 cut(s) 442, 991, 1985, 2055, 2058, 2061
ApoI RAATTY 3 cut(s) 203, 261, 1525
ArsI GACNNNNNNTTYG 2 cut(s) 1849, 1881
AspS9I GGNCC 6 cut(s) 101, 718, 759, 1026, 1226, 1682
AsuC2I CCSGG 2 cut(s) 1550, 1680
AsuHPI GGTGA 5 cut(s) 167, 404, 839, 1268, 1324
AvaI CYCGRG 1 cut(s) 65
AvaII GGWCC 5 cut(s) 101, 718, 759, 1226, 1682
BaeGI GKGCMC 2 cut(s) 229, 780
BamHI GGATCC 5 cut(s) 280, 500, 695, 1508, 1994
BanI GGYRCC 2 cut(s) 1057, 1411
BanII GRGCYC 2 cut(s) 966, 1777
BbsI GAAGAC 2 cut(s) 752, 1752
Bbv12I GWGCWC 4 cut(s) 229, 455, 1168, 1777
BbvI GCAGC 6 cut(s) 429, 1003, 1972, 2067, 2070, 2073
BccI CCATC 3 cut(s) 854, 899, 1154
BciT130I CCWGG 4 cut(s) 505, 555, 1587, 1797
BciVI GTATCC 1 cut(s) 847
BcnI CCSGG 2 cut(s) 1550, 1680
BcoDI GTCTC 1 cut(s) 1139
BcuI ACTAGT 1 cut(s) 410
BfaI CTAG 4 cut(s) 411, 1530, 1670, 1730
BfmI CTRYAG 2 cut(s) 975, 1197
BfuAI ACCTGC 2 cut(s) 1190, 1214
BfuI GTATCC 1 cut(s) 847
BglII AGATCT 2 cut(s) 1237, 1427
BisI GCNGC 7 cut(s) 443, 992, 1966, 1986, 2056, 2059, 2062
BlsI GCNGC 7 cut(s) 444, 993, 1967, 1987, 2057, 2060, 2063
BmcAI AGTACT 1 cut(s) 1296
Bme1390I CCNGG 6 cut(s) 505, 555, 1550, 1587, 1680, 1797
Bme18I GGWCC 5 cut(s) 101, 718, 759, 1226, 1682
BmeT110I CYCGRG 1 cut(s) 65
BmgBI CACGTC 1 cut(s) 1360
BmgT120I GGNCC 6 cut(s) 101, 718, 759, 1026, 1226, 1682
BmrFI CCNGG 6 cut(s) 505, 555, 1550, 1587, 1680, 1797
BmrI ACTGGG 1 cut(s) 1033
BmsI GCATC 9 cut(s) 68, 90, 295, 1148, 1187, 1210, 1794, 1851, 1927
BmuI ACTGGG 1 cut(s) 1033
BoxI GACNNNNGTC 1 cut(s) 134
BpiI GAAGAC 2 cut(s) 752, 1752
BpmI CTGGAG 4 cut(s) 594, 1106, 1599, 1773
Bpu10I CCTNAGC 1 cut(s) 1620
BpuEI CTTGAG 3 cut(s) 604, 685, 1047
BpuMI CCSGG 2 cut(s) 1550, 1680
BsaHI GRCGYC 1 cut(s) 1661
BsaJI CCNNGG 3 cut(s) 714, 1585, 1901
BsaXI ACNNNNNCTCC 6 cut(s) 529, 559, 1771, 1801, 2065, 2095
Bsc4I CCNNNNNNNGG 8 cut(s) 308, 756, 824, 1202, 1395, 1622, 1796, 2090
Bse118I RCCGGY 2 cut(s) 308, 1663
Bse1I ACTGG 6 cut(s) 373, 394, 898, 1028, 1413, 1790
Bse3DI GCAATG 1 cut(s) 1763
BseBI CCWGG 4 cut(s) 505, 555, 1587, 1797
BseDI CCNNGG 3 cut(s) 714, 1585, 1901
BseGI GGATG 1 cut(s) 910
BseLI CCNNNNNNNGG 8 cut(s) 308, 756, 824, 1202, 1395, 1622, 1796, 2090
BseMI GCAATG 1 cut(s) 1763
BseMII CTCAG 6 cut(s) 299, 340, 447, 1134, 1634, 2024
BseNI ACTGG 6 cut(s) 373, 394, 898, 1028, 1413, 1790
BseRI GAGGAG 6 cut(s) 391, 412, 433, 473, 636, 804
BseSI GKGCMC 2 cut(s) 229, 780
BseXI GCAGC 6 cut(s) 429, 1003, 1972, 2067, 2070, 2073
BseYI CCCAGC 3 cut(s) 1213, 2044, 2051
Bsh1236I CGCG 1 cut(s) 1658
Bsh1285I CGRYCG 1 cut(s) 918
BshFI GGCC 1 cut(s) 1028
BshNI GGYRCC 2 cut(s) 1057, 1411
BsiEI CGRYCG 1 cut(s) 918
BsiHKAI GWGCWC 4 cut(s) 229, 455, 1168, 1777
BsiHKCI CYCGRG 1 cut(s) 65
BsiSI CCGG 4 cut(s) 309, 1550, 1664, 1679
BslFI GGGAC 3 cut(s) 114, 745, 1991
BslI CCNNNNNNNGG 8 cut(s) 308, 756, 824, 1202, 1395, 1622, 1796, 2090
BsmAI GTCTC 1 cut(s) 1139
BsmFI GGGAC 3 cut(s) 114, 745, 1991
BsmI GAATGC 1 cut(s) 916
BsnI GGCC 1 cut(s) 1028
BsoBI CYCGRG 1 cut(s) 65
Bsp1286I GDGCHC 6 cut(s) 229, 455, 780, 966, 1168, 1777
Bsp1407I TGTACA 1 cut(s) 140
BspACI CCGC 7 cut(s) 104, 750, 1335, 1344, 1656, 1952, 1965
BspANI GGCC 1 cut(s) 1028
BspCNI CTCAG 6 cut(s) 298, 339, 448, 1135, 1633, 2023
BspFNI CGCG 1 cut(s) 1658
BspMAI CTGCAG 1 cut(s) 1201
BspMI ACCTGC 2 cut(s) 1190, 1214
BspT107I GGYRCC 2 cut(s) 1057, 1411
BsrDI GCAATG 1 cut(s) 1763
BsrFI RCCGGY 2 cut(s) 308, 1663
BsrGI TGTACA 1 cut(s) 140
BsrI ACTGG 6 cut(s) 373, 394, 898, 1028, 1413, 1790
BssAI RCCGGY 2 cut(s) 308, 1663
BssECI CCNNGG 3 cut(s) 714, 1585, 1901
BssNI GRCGYC 1 cut(s) 1661
BssT1I CCWWGG 1 cut(s) 714
Bst2UI CCWGG 4 cut(s) 505, 555, 1587, 1797
Bst4CI ACNGT 2 cut(s) 1267, 1493
BstACI GRCGYC 1 cut(s) 1661
BstAPI GCANNNNNTGC 1 cut(s) 87
BstAUI TGTACA 1 cut(s) 140
BstC8I GCNNGC 5 cut(s) 252, 1197, 1604, 1665, 1809
BstDEI CTNAG 8 cut(s) 285, 326, 456, 1143, 1298, 1620, 2010, 2068
BstF5I GGATG 1 cut(s) 910
BstFNI CGCG 1 cut(s) 1658
BstMAI GTCTC 1 cut(s) 1139
BstMCI CGRYCG 1 cut(s) 918
BstMWI GCNNNNNNNGC 8 cut(s) 87, 233, 439, 1376, 1385, 1664, 1971, 2061
BstNI CCWGG 4 cut(s) 505, 555, 1587, 1797
BstNSI RCATGY 4 cut(s) 347, 974, 1642, 1811
BstPAI GACNNNNGTC 1 cut(s) 134
BstSCI CCNGG 6 cut(s) 503, 553, 1548, 1585, 1678, 1795
BstSFI CTRYAG 2 cut(s) 975, 1197
BstSLI GKGCMC 2 cut(s) 229, 780
BstUI CGCG 1 cut(s) 1658
BstV1I GCAGC 6 cut(s) 429, 1003, 1972, 2067, 2070, 2073
BstV2I GAAGAC 2 cut(s) 752, 1752
BstX2I RGATCY 7 cut(s) 280, 500, 695, 1237, 1427, 1508, 1994
BstXI CCANNNNNNTGG 1 cut(s) 482
BstYI RGATCY 7 cut(s) 280, 500, 695, 1237, 1427, 1508, 1994
BsuI GTATCC 1 cut(s) 847
BsuRI GGCC 1 cut(s) 1028
BtrI CACGTC 1 cut(s) 1360
BtsCI GGATG 1 cut(s) 910
BtsI GCAGTG 1 cut(s) 1504
BtsIMutI CAGTG 8 cut(s) 195, 366, 387, 453, 1263, 1406, 1498, 1504
BveI ACCTGC 2 cut(s) 1190, 1214
Cac8I GCNNGC 5 cut(s) 252, 1197, 1604, 1665, 1809
CaiI CAGNNNCTG 2 cut(s) 1229, 1586
Cfr10I RCCGGY 2 cut(s) 308, 1663
Cfr13I GGNCC 6 cut(s) 101, 718, 759, 1026, 1226, 1682
CseI GACGC 2 cut(s) 1669, 2023
Csp6I GTAC 7 cut(s) 141, 158, 289, 1295, 1302, 1633, 1793
CviQI GTAC 7 cut(s) 141, 158, 289, 1295, 1302, 1633, 1793
DdeI CTNAG 8 cut(s) 285, 326, 456, 1143, 1298, 1620, 2010, 2068
DraI TTTAAA 2 cut(s) 57, 271
Ecl136II GAGCTC 1 cut(s) 1775
Eco130I CCWWGG 1 cut(s) 714
Eco24I GRGCYC 2 cut(s) 966, 1777
Eco47I GGWCC 5 cut(s) 101, 718, 759, 1226, 1682
Eco53kI GAGCTC 1 cut(s) 1775
Eco57I CTGAAG 1 cut(s) 1581
Eco88I CYCGRG 1 cut(s) 65
EcoICRI GAGCTC 1 cut(s) 1775
EcoO109I RGGNCCY 2 cut(s) 759, 1226
EcoRI GAATTC 1 cut(s) 1525
EcoRII CCWGG 4 cut(s) 503, 553, 1585, 1795
EcoT14I CCWWGG 1 cut(s) 714
EcoT22I ATGCAT 3 cut(s) 83, 1141, 1809
EcoT38I GRGCYC 2 cut(s) 966, 1777
ErhI CCWWGG 1 cut(s) 714
FaqI GGGAC 3 cut(s) 114, 745, 1991
FblI GTMKAC 1 cut(s) 384
Fnu4HI GCNGC 7 cut(s) 443, 992, 1966, 1986, 2056, 2059, 2062
FokI GGATG 1 cut(s) 917
FriOI GRGCYC 2 cut(s) 966, 1777
Fsp4HI GCNGC 7 cut(s) 443, 992, 1966, 1986, 2056, 2059, 2062
FspBI CTAG 4 cut(s) 411, 1530, 1670, 1730
GluI GCNGC 7 cut(s) 443, 992, 1966, 1986, 2056, 2059, 2062
GsaI CCCAGC 3 cut(s) 1217, 2048, 2055
GsuI CTGGAG 4 cut(s) 594, 1106, 1599, 1773
HaeIII GGCC 1 cut(s) 1028
HapII CCGG 4 cut(s) 309, 1550, 1664, 1679
HgaI GACGC 2 cut(s) 1669, 2023
Hin1I GRCGYC 1 cut(s) 1661
HincII GTYRAC 2 cut(s) 1110, 1173
HindII GTYRAC 2 cut(s) 1110, 1173
HindIII AAGCTT 2 cut(s) 934, 1593
HpaII CCGG 4 cut(s) 309, 1550, 1664, 1679
HphI GGTGA 5 cut(s) 167, 404, 839, 1268, 1324
Hpy166II GTNNAC 7 cut(s) 171, 227, 385, 488, 1110, 1173, 1304
Hpy188I TCNGA 9 cut(s) 466, 849, 879, 961, 1144, 1779, 1902, 2039, 2085
Hpy188III TCNNGA 8 cut(s) 65, 127, 164, 328, 611, 621, 902, 1085
Hpy8I GTNNAC 7 cut(s) 171, 227, 385, 488, 1110, 1173, 1304
Hpy99I CGWCG 1 cut(s) 1663
HpyCH4III ACNGT 2 cut(s) 1267, 1493
HpyCH4IV ACGT 4 cut(s) 652, 691, 807, 1359
HpyF10VI GCNNNNNNNGC 8 cut(s) 87, 233, 439, 1376, 1385, 1664, 1971, 2061
HpyF3I CTNAG 8 cut(s) 285, 326, 456, 1143, 1298, 1620, 2010, 2068
HpySE526I ACGT 4 cut(s) 652, 691, 807, 1359
Hsp92I GRCGYC 1 cut(s) 1661
KroI GCCGGC 1 cut(s) 1663
KroNI GCCGGC 1 cut(s) 1665
LmnI GCTCC 8 cut(s) 438, 507, 1171, 1376, 1502, 1618, 1772, 1792
Lsp1109I GCAGC 6 cut(s) 429, 1003, 1972, 2067, 2070, 2073
LweI GCATC 9 cut(s) 68, 90, 295, 1148, 1187, 1210, 1794, 1851, 1927
MaeI CTAG 4 cut(s) 411, 1530, 1670, 1730
MaeII ACGT 4 cut(s) 652, 691, 807, 1359
MaeIII GTNAC 3 cut(s) 1256, 1571, 1732
MboII GAAGA 8 cut(s) 32, 227, 308, 573, 603, 757, 1730, 1752
MfeI CAATTG 1 cut(s) 986
MflI RGATCY 7 cut(s) 280, 500, 695, 1237, 1427, 1508, 1994
MhlI GDGCHC 6 cut(s) 229, 455, 780, 966, 1168, 1777
MlyI GAGTC 4 cut(s) 318, 470, 1097, 1850
MmeI TCCRAC 2 cut(s) 510, 1354
Mph1103I ATGCAT 3 cut(s) 83, 1141, 1809
MroNI GCCGGC 1 cut(s) 1663
MseI TTAA 7 cut(s) 56, 207, 270, 569, 786, 1713, 1946
MslI CAYNNNNRTG 6 cut(s) 348, 495, 519, 1158, 1859, 2104
MspA1I CMGCKG 2 cut(s) 1217, 1478
MspI CCGG 4 cut(s) 309, 1550, 1664, 1679
MspR9I CCNGG 6 cut(s) 505, 555, 1550, 1587, 1680, 1797
MunI CAATTG 1 cut(s) 986
Mva1269I GAATGC 1 cut(s) 916
MvaI CCWGG 4 cut(s) 505, 555, 1587, 1797
MvnI CGCG 1 cut(s) 1658
MwoI GCNNNNNNNGC 8 cut(s) 87, 233, 439, 1376, 1385, 1664, 1971, 2061
NaeI GCCGGC 1 cut(s) 1665
NciI CCSGG 2 cut(s) 1550, 1680
NgoMIV GCCGGC 1 cut(s) 1663
NmeAIII GCCGAG 1 cut(s) 1414
NmuCI GTSAC 1 cut(s) 1256
NsiI ATGCAT 3 cut(s) 83, 1141, 1809
NspI RCATGY 4 cut(s) 347, 974, 1642, 1811
PaeI GCATGC 1 cut(s) 1811
PaqCI CACCTGC 1 cut(s) 1190
PctI GAATGC 1 cut(s) 916
PdiI GCCGGC 1 cut(s) 1665
PfeI GAWTC 6 cut(s) 676, 710, 956, 980, 1471, 1850
PflMI CCANNNNNTGG 1 cut(s) 824
PfoI TCCNGGA 2 cut(s) 503, 553
PkrI GCNGC 7 cut(s) 444, 993, 1967, 1987, 2057, 2060, 2063
PleI GAGTC 4 cut(s) 318, 469, 1096, 1849
PpsI GAGTC 4 cut(s) 318, 469, 1096, 1849
PpuMI RGGWCCY 2 cut(s) 759, 1226
PshAI GACNNNNGTC 1 cut(s) 134
PsiI TTATAA 2 cut(s) 338, 638
Psp124BI GAGCTC 1 cut(s) 1777
Psp5II RGGWCCY 2 cut(s) 759, 1226
Psp6I CCWGG 4 cut(s) 503, 553, 1585, 1795
PspFI CCCAGC 3 cut(s) 1213, 2044, 2051
PspGI CCWGG 4 cut(s) 503, 553, 1585, 1795
PspPI GGNCC 6 cut(s) 101, 718, 759, 1026, 1226, 1682
PspPPI RGGWCCY 2 cut(s) 759, 1226
PstI CTGCAG 1 cut(s) 1201
PstNI CAGNNNCTG 2 cut(s) 1229, 1586
PsuI RGATCY 7 cut(s) 280, 500, 695, 1237, 1427, 1508, 1994
PvuII CAGCTG 2 cut(s) 1217, 1478
RsaI GTAC 7 cut(s) 142, 159, 290, 1296, 1303, 1634, 1794
RsaNI GTAC 7 cut(s) 141, 158, 289, 1295, 1302, 1633, 1793
RseI CAYNNNNRTG 6 cut(s) 348, 495, 519, 1158, 1859, 2104
SacI GAGCTC 1 cut(s) 1777
SaqAI TTAA 7 cut(s) 56, 207, 270, 569, 786, 1713, 1946
SatI GCNGC 7 cut(s) 443, 992, 1966, 1986, 2056, 2059, 2062
Sau96I GGNCC 6 cut(s) 101, 718, 759, 1026, 1226, 1682
SbfI CCTGCAGG 1 cut(s) 1201
ScaI AGTACT 1 cut(s) 1296
SchI GAGTC 4 cut(s) 318, 470, 1097, 1850
ScrFI CCNGG 6 cut(s) 505, 555, 1550, 1587, 1680, 1797
SdaI CCTGCAGG 1 cut(s) 1201
SduI GDGCHC 6 cut(s) 229, 455, 780, 966, 1168, 1777
SfaNI GCATC 9 cut(s) 68, 90, 295, 1148, 1187, 1210, 1794, 1851, 1927
SfcI CTRYAG 2 cut(s) 975, 1197
SinI GGWCC 5 cut(s) 101, 718, 759, 1226, 1682
SmiMI CAYNNNNRTG 6 cut(s) 348, 495, 519, 1158, 1859, 2104
SmlI CTYRAG 3 cut(s) 619, 700, 1062
SmoI CTYRAG 3 cut(s) 619, 700, 1062
SpeI ACTAGT 1 cut(s) 410
SphI GCATGC 1 cut(s) 1811
Sse8387I CCTGCAGG 1 cut(s) 1201
SsiI CCGC 7 cut(s) 104, 750, 1335, 1344, 1656, 1952, 1965
SspMI CTAG 4 cut(s) 411, 1530, 1670, 1730
SstI GAGCTC 1 cut(s) 1777
StyD4I CCNGG 6 cut(s) 503, 553, 1548, 1585, 1678, 1795
StyI CCWWGG 1 cut(s) 714
TaaI ACNGT 2 cut(s) 1267, 1493
TaiI ACGT 4 cut(s) 655, 694, 810, 1362
TaqI TCGA 2 cut(s) 601, 1814
TaqII GACCGA 1 cut(s) 932
TatI WGTACW 4 cut(s) 140, 157, 1294, 1301
TauI GCSGC 1 cut(s) 1968
TfiI GAWTC 6 cut(s) 676, 710, 956, 980, 1471, 1850
Tru1I TTAA 7 cut(s) 56, 207, 270, 569, 786, 1713, 1946
Tru9I TTAA 7 cut(s) 56, 207, 270, 569, 786, 1713, 1946
TscAI CASTG 8 cut(s) 195, 373, 394, 460, 1270, 1413, 1498, 1504
TseFI GTSAC 1 cut(s) 1256
TseI GCWGC 6 cut(s) 442, 991, 1985, 2055, 2058, 2061
Tsp45I GTSAC 1 cut(s) 1256
TspDTI ATGAA 7 cut(s) 308, 483, 600, 1538, 1731, 1752, 1863
TspGWI ACGGA 3 cut(s) 550, 1064, 1158
TspRI CASTG 8 cut(s) 195, 373, 394, 460, 1270, 1413, 1498, 1504
Van91I CCANNNNNTGG 1 cut(s) 824
VneI GTGCAC 1 cut(s) 225
VpaK11BI GGWCC 5 cut(s) 101, 718, 759, 1226, 1682
XapI RAATTY 3 cut(s) 203, 261, 1525
XceI RCATGY 4 cut(s) 347, 974, 1642, 1811
XcmI CCANNNNNNNNNTGG 1 cut(s) 482
XmiI GTMKAC 1 cut(s) 384
XspI CTAG 4 cut(s) 411, 1530, 1670, 1730
ZrmI AGTACT 1 cut(s) 1296
Zsp2I ATGCAT 3 cut(s) 83, 1141, 1809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.