heptahelical
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 27 |
| AflIII | ACRYGT | 1 cut(s) | 14 |
| ApeKI | GCWGC | 1 cut(s) | 6 |
| AsuHPI | GGTGA | 1 cut(s) | 32 |
| BbvI | GCAGC | 1 cut(s) | 18 |
| BciVI | GTATCC | 1 cut(s) | 137 |
| BfaI | CTAG | 1 cut(s) | 142 |
| BfuI | GTATCC | 1 cut(s) | 137 |
| BisI | GCNGC | 1 cut(s) | 7 |
| BlsI | GCNGC | 1 cut(s) | 8 |
| BseXI | GCAGC | 1 cut(s) | 18 |
| BsmI | GAATGC | 1 cut(s) | 29 |
| BspACI | CCGC | 1 cut(s) | 27 |
| Bst6I | CTCTTC | 1 cut(s) | 97 |
| BstNSI | RCATGY | 1 cut(s) | 18 |
| BstV1I | GCAGC | 1 cut(s) | 18 |
| BsuI | GTATCC | 1 cut(s) | 137 |
| BtsI | GCAGTG | 1 cut(s) | 93 |
| BtsIMutI | CAGTG | 1 cut(s) | 93 |
| CspCI | CAANNNNNGTGG | 1 cut(s) | 33 |
| CviAII | CATG | 1 cut(s) | 15 |
| Eam1104I | CTCTTC | 1 cut(s) | 97 |
| EarI | CTCTTC | 1 cut(s) | 97 |
| FaeI | CATG | 1 cut(s) | 18 |
| FaiI | YATR | 4 cut(s) | 16, 113, 115, 132 |
| FatI | CATG | 1 cut(s) | 14 |
| Fnu4HI | GCNGC | 1 cut(s) | 7 |
| Fsp4HI | GCNGC | 1 cut(s) | 7 |
| FspBI | CTAG | 1 cut(s) | 142 |
| FspEI | CC | 8 cut(s) | 3, 12, 19, 20, 35, 43, 83, 108 |
| GluI | GCNGC | 1 cut(s) | 7 |
| Hin1II | CATG | 1 cut(s) | 18 |
| HinfI | GANTC | 1 cut(s) | 97 |
| HphI | GGTGA | 1 cut(s) | 32 |
| Hsp92II | CATG | 1 cut(s) | 18 |
| LmnI | GCTCC | 1 cut(s) | 60 |
| LpnPI | CCDG | 1 cut(s) | 108 |
| Lsp1109I | GCAGC | 1 cut(s) | 18 |
| MaeI | CTAG | 1 cut(s) | 142 |
| MaeIII | GTNAC | 1 cut(s) | 79 |
| MboII | GAAGA | 2 cut(s) | 114, 119 |
| MlyI | GAGTC | 1 cut(s) | 106 |
| MnlI | CCTC | 1 cut(s) | 43 |
| Mva1269I | GAATGC | 1 cut(s) | 29 |
| NlaIII | CATG | 1 cut(s) | 18 |
| NspI | RCATGY | 1 cut(s) | 18 |
| PciI | ACATGT | 1 cut(s) | 14 |
| PctI | GAATGC | 1 cut(s) | 29 |
| PkrI | GCNGC | 1 cut(s) | 8 |
| PleI | GAGTC | 1 cut(s) | 105 |
| PpsI | GAGTC | 1 cut(s) | 105 |
| PscI | ACATGT | 1 cut(s) | 14 |
| SatI | GCNGC | 1 cut(s) | 7 |
| SchI | GAGTC | 1 cut(s) | 106 |
| SetI | ASST | 1 cut(s) | 127 |
| SgeI | CNNG | 3 cut(s) | 27, 83, 135 |
| SsiI | CCGC | 1 cut(s) | 27 |
| SspMI | CTAG | 1 cut(s) | 142 |
| TaqI | TCGA | 1 cut(s) | 100 |
| TscAI | CASTG | 1 cut(s) | 93 |
| TseI | GCWGC | 1 cut(s) | 6 |
| TspRI | CASTG | 1 cut(s) | 93 |
| XceI | RCATGY | 1 cut(s) | 18 |
| XspI | CTAG | 1 cut(s) | 142 |