AT5G03470

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012260
Physical Location & Seq
Forward (+)
866339 .. 869252
2914 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G03470.1

Sequence Viewer

Length: 1488 bp
ATGTTTAAGAAGATCATGAAAGGGGCAAATCGAAAAGCCTCTAAAGCTGAGGCGAATGATTCGTCTATGTATGGGTTTGATCCACCAGGTAGGTCAGGTCCTGGATCCAATATGATTGTAAATCACGCATCTCGTGGTTCGCTTGTTCCTTCTTCTCCAAATTCAATGGCGGCGGCCACCACACAACCGCCTCCTATGTATTCAGTCGAGCCTCTTCCCTTGTTCAGAGACGTCTCTGTCTCCGAGCGTCAATCCTTGTTCTTGAGGAAACTCCAGATTTGTTGTTTTCAATTTGATTTTACGGATACGTTGAAGAATGCGAGAGAGAAGGAGATTAAGAGGCAAACGTTATTGGAGCTAGTTGATTTTATACAGTCTGGAGCAGGGAAGCTCACTGAAGTTTGTCAAGAAGAAATGGTAAAGATGATTTCGGTTAATATATTCCGGTGTCTTCCTCCGGCTTCACACGAGAATACAGGTCAAGAGCCTGCTGATCTTGAGGAAGAGGAGCCTTATTTGGAACCATCTTGGCCTCATTTACAGCTAATTTATGAGTTGCTGCTGAGATACATTGTTCCTTCTGACACAGATACAAAAGTTGCAAAACGGTATATTGATCATTCTTTTGTGTTGAGGTTGCTTGAATTGTTTGAAACTGAAGATCCTAGAGAAAGGGAGTACTTGAAAACGATTCTTCATAGGATTTATGGCAAGTTTATGGTTCACAGGCCCTTTATTAGGAAAGCAATGAACCATATTTTCTATAGGTTTATTTATGAGACAGAGAGACATAGTGGGATTGGGGAGCTTTTGGAGATTCTCGGTAGTATTATTAATGGGTTTGCCTTGCCAATGAAGGAGGAGCACAAGCTCTTTTTGATTAGGGCGTTGATACCGCTGCATAAGCCAAAACCAATCGCTATGTATCACCAACAATTGTCTTACTGCATCGTTCAGTTTGTGGAGAAAGATTATAAGCTTGCGGATACGGTGATCAGGGGCTTGTTAAAGTTTTGGCCTGTGACAAATTGTACAAAAGAGGTTCTCTTTCTTGGTGAACTTGAAGAGGTTCTTGAGGCAACACAAACTGTTGAGTTCCAACGCTGTATGGTCCCTCTGTTCCAACAGATTGCTCGATGCCTCAGTAGCTCTAATTTTCAGGTTGCAGAACGAGCTCTGTTCTTGTGGAACAACGAGCATGTGGTGGGTCTAATAGCTCAGAACCGAGGAGTGATCCTTCCCATCATATTTGCATCCCTGGAGAAAAACATAGAGTCTCATTGGAACCAAGCGGTACACGGTCTAAGTGCGAATATAAAGAGAATGTTCATGGAGATGGACCCTGAGCTCTTTGAAGAATGTCAGCAACAGTACGAAGAAAAACAAGCCAAATCTAAACAAGTGGAAGAGCAACGCCAAAATAGGTGGAGGAGATTAGATGAAGCAGTGGAGGAACGAGAAAGAGAAGATCCTATGATCACTTCTTAG

Protein Analysis

495

Amino Acids

57.54

Weight (kDa)

6.12

Isoelectric Point (pI)

48.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B56 PF01603 72 - 482 5.2e-187 Protein phosphatase 2A regulatory B subunit (B56 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012260)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09880 AT5G03470
fragaria_vesca FvH4_6g16640 FvH4_6g16640 FvH4_6g16640
malus_domestica MD04G1090100.v1.1 MD12G1108600.v1.1
prunus_persica Prupe.7G127000_v2.0.a1
pyrus_communis pycom04g08520
rosa_chinensis RchiOBHm_Chr3g0470601
rosa_laevigata RLG00000024255
rosa_multiflora Rmu_sc0002115.1_g000016
rosa_roxburghii Rroxscaffold_6G00410700
rosa_rugosa Rorug03G0110400
rosa_samantha Rh3AG160400 Rh3BG184900 Rh3CG175400 Rh3DG189800
rosa_wichuraiana Rw3G015170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 975
AasI GACNNNNNNGTC 1 cut(s) 236
AatII GACGTC 1 cut(s) 234
AciI CCGC 6 cut(s) 170, 173, 188, 896, 983, 1292
AclI AACGTT 1 cut(s) 347
AclWI GGATC 6 cut(s) 74, 99, 112, 656, 1228, 1463
AcoI YGGCCR 1 cut(s) 174
AcsI RAATTY 1 cut(s) 160
AcuI CTGAAG 2 cut(s) 417, 678
AcyI GRCGYC 1 cut(s) 231
AfaI GTAC 4 cut(s) 680, 1033, 1296, 1373
AfiI CCNNNNNNNGG 1 cut(s) 738
AgsI TTSAA 8 cut(s) 165, 290, 313, 644, 653, 685, 1064, 1355
AjnI CCWGG 3 cut(s) 85, 100, 1257
Alw21I GWGCWC 3 cut(s) 867, 1177, 1350
Alw26I GTCTC 6 cut(s) 222, 238, 244, 773, 781, 1281
AlwI GGATC 6 cut(s) 74, 99, 112, 656, 1228, 1463
AlwNI CAGNNNCTG 1 cut(s) 101
AoxI GGCC 4 cut(s) 174, 530, 728, 1016
ApeKI GCWGC 2 cut(s) 559, 898
ApoI RAATTY 1 cut(s) 160
ArsI GACNNNNNNTTYG 2 cut(s) 47, 79
AseI ATTAAT 1 cut(s) 834
Asp700I GAANNNNTTC 1 cut(s) 1068
AspS9I GGNCC 4 cut(s) 98, 729, 1111, 1339
AsuHPI GGTGA 3 cut(s) 920, 1003, 1067
AvaII GGWCC 3 cut(s) 98, 1111, 1339
BaeI ACNNNNGTAYC 2 cut(s) 582, 615
BamHI GGATCC 1 cut(s) 104
BanII GRGCYC 2 cut(s) 1177, 1350
BauI CACGAG 2 cut(s) 132, 467
BbsI GAAGAC 1 cut(s) 443
Bbv12I GWGCWC 3 cut(s) 867, 1177, 1350
BbvCI CCTCAGC 1 cut(s) 48
BbvI GCAGC 2 cut(s) 546, 885
BccI CCATC 3 cut(s) 532, 1250, 1330
BciT130I CCWGG 3 cut(s) 87, 102, 1259
BciVI GTATCC 2 cut(s) 298, 979
BclI TGATCA 3 cut(s) 616, 993, 1476
BcoDI GTCTC 6 cut(s) 222, 238, 244, 773, 781, 1281
BfaI CTAG 2 cut(s) 359, 666
BfmI CTRYAG 1 cut(s) 763
BfuI GTATCC 2 cut(s) 298, 979
BisI GCNGC 4 cut(s) 171, 174, 560, 899
BlsI GCNGC 4 cut(s) 172, 175, 561, 900
BmcAI AGTACT 1 cut(s) 680
Bme1390I CCNGG 3 cut(s) 87, 102, 1259
Bme18I GGWCC 3 cut(s) 98, 1111, 1339
BmgT120I GGNCC 4 cut(s) 98, 729, 1111, 1339
BmiI GGNNCC 6 cut(s) 106, 510, 522, 1113, 1286, 1341
BmrFI CCNGG 3 cut(s) 87, 102, 1259
BmsI GCATC 4 cut(s) 137, 957, 1127, 1262
BpiI GAAGAC 1 cut(s) 443
BpmI CTGGAG 3 cut(s) 257, 399, 1280
Bpu10I CCTNAGC 2 cut(s) 48, 1344
BpuEI CTTGAG 3 cut(s) 283, 518, 1094
BsaHI GRCGYC 1 cut(s) 231
BsaJI CCNNGG 2 cut(s) 1225, 1257
BsaWI WCCGGW 1 cut(s) 444
Bsc4I CCNNNNNNNGG 1 cut(s) 738
Bse3DI GCAATG 1 cut(s) 753
BseBI CCWGG 3 cut(s) 87, 102, 1259
BseDI CCNNGG 2 cut(s) 1225, 1257
BseGI GGATG 1 cut(s) 1253
BseLI CCNNNNNNNGG 1 cut(s) 738
BseMI GCAATG 1 cut(s) 753
BseMII CTCAG 5 cut(s) 39, 554, 1156, 1232, 1335
BseRI GAGGAG 4 cut(s) 521, 875, 1242, 1444
BseXI GCAGC 2 cut(s) 546, 885
BshFI GGCC 4 cut(s) 176, 532, 730, 1018
BsiHKAI GWGCWC 3 cut(s) 867, 1177, 1350
BsiSI CCGG 2 cut(s) 445, 458
BslFI GGGAC 1 cut(s) 1097
BslI CCNNNNNNNGG 1 cut(s) 738
BsmAI GTCTC 6 cut(s) 222, 238, 244, 773, 781, 1281
BsmBI CGTCTC 2 cut(s) 222, 238
BsmFI GGGAC 1 cut(s) 1097
BsmI GAATGC 1 cut(s) 322
BsnI GGCC 4 cut(s) 176, 532, 730, 1018
Bsp1286I GDGCHC 3 cut(s) 867, 1177, 1350
Bsp1407I TGTACA 1 cut(s) 1031
BspACI CCGC 6 cut(s) 170, 173, 188, 896, 983, 1292
BspANI GGCC 4 cut(s) 176, 532, 730, 1018
BspCNI CTCAG 5 cut(s) 40, 555, 1155, 1231, 1336
BspHI TCATGA 1 cut(s) 15
BspLI GGNNCC 6 cut(s) 106, 510, 522, 1113, 1286, 1341
BspPI GGATC 6 cut(s) 74, 99, 112, 656, 1228, 1463
BspQI GCTCTTC 1 cut(s) 1401
BsrDI GCAATG 1 cut(s) 753
BsrGI TGTACA 1 cut(s) 1031
BssECI CCNNGG 2 cut(s) 1225, 1257
BssNI GRCGYC 1 cut(s) 231
BssSI CACGAG 2 cut(s) 132, 467
Bst2BI CACGAG 2 cut(s) 132, 467
Bst2UI CCWGG 3 cut(s) 87, 102, 1259
Bst4CI ACNGT 6 cut(s) 375, 609, 991, 1090, 1301, 1371
Bst6I CTCTTC 4 cut(s) 219, 498, 1059, 1401
BstACI GRCGYC 1 cut(s) 231
BstAUI TGTACA 1 cut(s) 1031
BstC8I GCNNGC 2 cut(s) 489, 981
BstDEI CTNAG 7 cut(s) 48, 563, 1142, 1218, 1304, 1344, 1485
BstENI CCTNNNNNAGG 1 cut(s) 736
BstF5I GGATG 1 cut(s) 1253
BstMAI GTCTC 6 cut(s) 222, 238, 244, 773, 781, 1281
BstMWI GCNNNNNNNGC 4 cut(s) 44, 904, 1146, 1172
BstNI CCWGG 3 cut(s) 87, 102, 1259
BstNSI RCATGY 1 cut(s) 1202
BstSCI CCNGG 3 cut(s) 85, 100, 1257
BstSFI CTRYAG 1 cut(s) 763
BstV1I GCAGC 2 cut(s) 546, 885
BstV2I GAAGAC 1 cut(s) 443
BstX2I RGATCY 3 cut(s) 104, 661, 1468
BstYI RGATCY 3 cut(s) 104, 661, 1468
BsuI GTATCC 2 cut(s) 298, 979
BsuRI GGCC 4 cut(s) 176, 532, 730, 1018
BtsCI GGATG 1 cut(s) 1253
BtsI GCAGTG 1 cut(s) 1452
BtsIMutI CAGTG 2 cut(s) 393, 1452
Cac8I GCNNGC 2 cut(s) 489, 981
CaiI CAGNNNCTG 1 cut(s) 101
CciI TCATGA 1 cut(s) 15
Cfr13I GGNCC 4 cut(s) 98, 729, 1111, 1339
CseI GACGC 1 cut(s) 236
CsiI ACCWGGT 1 cut(s) 85
Csp6I GTAC 4 cut(s) 679, 1032, 1295, 1372
CspCI CAANNNNNGTGG 2 cut(s) 1406, 1441
CviAII CATG 3 cut(s) 16, 1199, 1330
CviQI GTAC 4 cut(s) 679, 1032, 1295, 1372
DdeI CTNAG 7 cut(s) 48, 563, 1142, 1218, 1304, 1344, 1485
DrdI GACNNNNNNGTC 1 cut(s) 236
DseDI GACNNNNNNGTC 1 cut(s) 236
EaeI YGGCCR 1 cut(s) 174
Eam1104I CTCTTC 4 cut(s) 219, 498, 1059, 1401
EarI CTCTTC 4 cut(s) 219, 498, 1059, 1401
Ecl136II GAGCTC 2 cut(s) 1175, 1348
Eco24I GRGCYC 2 cut(s) 1177, 1350
Eco47I GGWCC 3 cut(s) 98, 1111, 1339
Eco53kI GAGCTC 2 cut(s) 1175, 1348
Eco57I CTGAAG 2 cut(s) 417, 678
EcoICRI GAGCTC 2 cut(s) 1175, 1348
EcoNI CCTNNNNNAGG 1 cut(s) 736
EcoO109I RGGNCCY 2 cut(s) 98, 729
EcoRII CCWGG 3 cut(s) 85, 100, 1257
EcoT38I GRGCYC 2 cut(s) 1177, 1350
Esp3I CGTCTC 2 cut(s) 222, 238
FaeI CATG 3 cut(s) 19, 1202, 1333
FalI AAGNNNNNCTT 2 cut(s) 1056, 1088
FaqI GGGAC 1 cut(s) 1097
FatI CATG 3 cut(s) 15, 1198, 1329
FbaI TGATCA 3 cut(s) 616, 993, 1476
Fnu4HI GCNGC 4 cut(s) 171, 174, 560, 899
FokI GGATG 1 cut(s) 1240
FriOI GRGCYC 2 cut(s) 1177, 1350
Fsp4HI GCNGC 4 cut(s) 171, 174, 560, 899
FspBI CTAG 2 cut(s) 359, 666
GluI GCNGC 4 cut(s) 171, 174, 560, 899
GsuI CTGGAG 3 cut(s) 257, 399, 1280
HaeIII GGCC 4 cut(s) 176, 532, 730, 1018
HapII CCGG 2 cut(s) 445, 458
HgaI GACGC 1 cut(s) 236
Hin1I GRCGYC 1 cut(s) 231
Hin1II CATG 3 cut(s) 19, 1202, 1333
HindIII AAGCTT 1 cut(s) 977
HinfI GANTC 4 cut(s) 59, 691, 817, 1274
HpaII CCGG 2 cut(s) 445, 458
HphI GGTGA 3 cut(s) 920, 1003, 1067
Hpy166II GTNNAC 3 cut(s) 724, 1058, 1297
Hpy188I TCNGA 4 cut(s) 227, 244, 583, 1221
Hpy188III TCNNGA 8 cut(s) 16, 262, 274, 378, 407, 482, 497, 1073
Hpy8I GTNNAC 3 cut(s) 724, 1058, 1297
HpyAV CCTTC 5 cut(s) 159, 322, 588, 850, 1247
HpyCH4III ACNGT 6 cut(s) 375, 609, 991, 1090, 1301, 1371
HpyCH4IV ACGT 3 cut(s) 231, 308, 347
HpyCH4V TGCA 5 cut(s) 602, 901, 948, 1166, 1253
HpyF10VI GCNNNNNNNGC 4 cut(s) 44, 904, 1146, 1172
HpyF3I CTNAG 7 cut(s) 48, 563, 1142, 1218, 1304, 1344, 1485
HpySE526I ACGT 3 cut(s) 231, 308, 347
Hsp92I GRCGYC 1 cut(s) 231
Hsp92II CATG 3 cut(s) 19, 1202, 1333
Ksp22I TGATCA 3 cut(s) 616, 993, 1476
LguI GCTCTTC 1 cut(s) 1401
LmnI GCTCC 5 cut(s) 355, 380, 508, 805, 862
Lsp1109I GCAGC 2 cut(s) 546, 885
LweI GCATC 4 cut(s) 137, 957, 1127, 1262
MabI ACCWGGT 1 cut(s) 85
MaeI CTAG 2 cut(s) 359, 666
MaeII ACGT 3 cut(s) 231, 308, 347
MaeIII GTNAC 1 cut(s) 1021
MfeI CAATTG 1 cut(s) 935
MflI RGATCY 3 cut(s) 104, 661, 1468
MhlI GDGCHC 3 cut(s) 867, 1177, 1350
MluCI AATT 7 cut(s) 160, 290, 546, 644, 935, 1027, 1153
MlyI GAGTC 1 cut(s) 1283
MmeI TCCRAC 2 cut(s) 1123, 1147
MroXI GAANNNNTTC 1 cut(s) 1068
MseI TTAA 5 cut(s) 6, 336, 435, 834, 1007
MslI CAYNNNNRTG 1 cut(s) 1334
MspA1I CMGCKG 1 cut(s) 898
MspI CCGG 2 cut(s) 445, 458
MspR9I CCNGG 3 cut(s) 87, 102, 1259
MunI CAATTG 1 cut(s) 935
Mva1269I GAATGC 1 cut(s) 322
MvaI CCWGG 3 cut(s) 87, 102, 1259
MwoI GCNNNNNNNGC 4 cut(s) 44, 904, 1146, 1172
NlaIII CATG 3 cut(s) 19, 1202, 1333
NlaIV GGNNCC 6 cut(s) 106, 510, 522, 1113, 1286, 1341
NmuCI GTSAC 1 cut(s) 1021
NspI RCATGY 1 cut(s) 1202
PagI TCATGA 1 cut(s) 15
PciSI GCTCTTC 1 cut(s) 1401
PctI GAATGC 1 cut(s) 322
PdmI GAANNNNTTC 1 cut(s) 1068
PfeI GAWTC 3 cut(s) 59, 691, 817
PfoI TCCNGGA 1 cut(s) 100
PkrI GCNGC 4 cut(s) 172, 175, 561, 900
PleI GAGTC 1 cut(s) 1282
PpsI GAGTC 1 cut(s) 1282
PpuMI RGGWCCY 1 cut(s) 98
PshBI ATTAAT 1 cut(s) 834
PsiI TTATAA 1 cut(s) 975
Psp124BI GAGCTC 2 cut(s) 1177, 1350
Psp1406I AACGTT 1 cut(s) 347
Psp5II RGGWCCY 1 cut(s) 98
Psp6I CCWGG 3 cut(s) 85, 100, 1257
PspGI CCWGG 3 cut(s) 85, 100, 1257
PspN4I GGNNCC 6 cut(s) 106, 510, 522, 1113, 1286, 1341
PspPI GGNCC 4 cut(s) 98, 729, 1111, 1339
PspPPI RGGWCCY 1 cut(s) 98
PstNI CAGNNNCTG 1 cut(s) 101
PsuI RGATCY 3 cut(s) 104, 661, 1468
RsaI GTAC 4 cut(s) 680, 1033, 1296, 1373
RsaNI GTAC 4 cut(s) 679, 1032, 1295, 1372
RseI CAYNNNNRTG 1 cut(s) 1334
SacI GAGCTC 2 cut(s) 1177, 1350
SapI GCTCTTC 1 cut(s) 1401
SaqAI TTAA 5 cut(s) 6, 336, 435, 834, 1007
SatI GCNGC 4 cut(s) 171, 174, 560, 899
Sau96I GGNCC 4 cut(s) 98, 729, 1111, 1339
ScaI AGTACT 1 cut(s) 680
SchI GAGTC 1 cut(s) 1283
ScrFI CCNGG 3 cut(s) 87, 102, 1259
SduI GDGCHC 3 cut(s) 867, 1177, 1350
SexAI ACCWGGT 1 cut(s) 85
SfaNI GCATC 4 cut(s) 137, 957, 1127, 1262
SfcI CTRYAG 1 cut(s) 763
SinI GGWCC 3 cut(s) 98, 1111, 1339
SmiMI CAYNNNNRTG 1 cut(s) 1334
SmlI CTYRAG 3 cut(s) 262, 497, 1073
SmoI CTYRAG 3 cut(s) 262, 497, 1073
Sse9I AATT 7 cut(s) 160, 290, 546, 644, 935, 1027, 1153
SsiI CCGC 6 cut(s) 170, 173, 188, 896, 983, 1292
SspMI CTAG 2 cut(s) 359, 666
SstI GAGCTC 2 cut(s) 1177, 1350
StyD4I CCNGG 3 cut(s) 85, 100, 1257
TaaI ACNGT 6 cut(s) 375, 609, 991, 1090, 1301, 1371
TaiI ACGT 3 cut(s) 234, 311, 350
TaqI TCGA 3 cut(s) 31, 207, 1135
TasI AATT 7 cut(s) 160, 290, 546, 644, 935, 1027, 1153
TatI WGTACW 2 cut(s) 678, 1031
TauI GCSGC 2 cut(s) 173, 176
TfiI GAWTC 3 cut(s) 59, 691, 817
Tru1I TTAA 5 cut(s) 6, 336, 435, 834, 1007
Tru9I TTAA 5 cut(s) 6, 336, 435, 834, 1007
TscAI CASTG 2 cut(s) 400, 1452
TseFI GTSAC 1 cut(s) 1021
TseI GCWGC 2 cut(s) 559, 898
Tsp45I GTSAC 1 cut(s) 1021
TspDTI ATGAA 6 cut(s) 32, 686, 764, 869, 1318, 1455
TspGWI ACGGA 1 cut(s) 317
TspRI CASTG 2 cut(s) 400, 1452
VpaK11BI GGWCC 3 cut(s) 98, 1111, 1339
VspI ATTAAT 1 cut(s) 834
XagI CCTNNNNNAGG 1 cut(s) 736
XapI RAATTY 1 cut(s) 160
XceI RCATGY 1 cut(s) 1202
XmnI GAANNNNTTC 1 cut(s) 1068
XspI CTAG 2 cut(s) 359, 666
ZraI GACGTC 1 cut(s) 232
ZrmI AGTACT 1 cut(s) 680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.