pycom04g08520

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0012260
Physical Location & Seq
Reverse (-)
10227256 .. 10230061
2806 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g08520.2

Sequence Viewer

Length: 1140 bp
ATGAGACTCATCGATCCTTCTTCGTACGGTTACGGGCCGCCCGGAACCCGCAATTCCGGGGCGGTCTCGGCGAATGTGGTCGTGAACCACGCCTCCCGGACTGGGCCAGCTTCTTCTGGCCCCAACGCGGGTGGCGTAGGTGTTACGGCGTTGCCGCCTTCCGGAACCATCGAGCCTCTCCCTATGTTCCGTGATGTGCCCGTCTCGGAGCGCCAGCACCTGTTCCTCCGCAAATTGCAGGTTTGTTGCTTTCAATTCGATTTTTCTGATACCCTTAAGACCCCTAGGGAGAAAGAAATTAAGCGTCAAACCCTTTTGGAATTGGTTGATTTTATACAATCTGGGTCGGGTAAGATCACCGAGACTTGTCAGGAGGAGATGATTAAAATGGTGTCTGTTAATATATTTCGGTGCTTGCCCCCTGCATCCCACGAAAACTCGGGCCAAGAGAATACCGATCCTGAAGAGGAGGAGCTTTATTTGGAGCCATCCTGGCCTCATTTGCAGTTGGTTTATGAGCTTCTTCTTAGGTACATTGTGTCATCTGATACCGATACCAAGGTTGCCAAGCGTTATATTGATCACTCATTTGTGTTGAAGTTGCTTGATTTGTTTGATTCTGAGGACCCGCGTGAGCGCGAGTACTTGAAGAACATTCTTCACCGCATATATGGGAAGTTCATGGTGCACCGACCCTTCATCAGGAAAGCCATCAATAACATATTCTATAGGTTTATATATGAGACCGAGAGGCACAGTGGCATTGCTGAGCTTTTAGAGATTCTTGGGAGTATAATTAATGGGTTTGCACTTCCAATGAAAGAGGAGCATAAGTTGTTTCTTGTTCGGGCACTTATACCGCTGCATAAGCCGAAGTCAATTGGTATGTATCATCAGCAGCTTTCCTACTGCATAATCCAGTTTGTTGAAAAGGATTACAAGTTGGCAGATACGGTTGTAAGGGGTTTGTTGAAATACTGGCCAGTGACCAACTGTCAGAAGGAAGTTCTTTTCCTTGGAGAACTTGAAGAAGTTCTGGAGGCAACGCAAGCTGCCGAGTTTCAGCGGTGCATGGTTCCACTTTTCAGACAGATTGCCCGCTGCCTCAACAGCTCTCATTTTCAGGCTTGCTGTATTTGA

Protein Analysis

380

Amino Acids

43.37

Weight (kDa)

6.47

Isoelectric Point (pI)

40.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012260)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09880 AT5G03470
fragaria_vesca FvH4_6g16640 FvH4_6g16640 FvH4_6g16640
malus_domestica MD04G1090100.v1.1 MD12G1108600.v1.1
prunus_persica Prupe.7G127000_v2.0.a1
pyrus_communis pycom04g08520
rosa_chinensis RchiOBHm_Chr3g0470601
rosa_laevigata RLG00000024255
rosa_multiflora Rmu_sc0002115.1_g000016
rosa_roxburghii Rroxscaffold_6G00410700
rosa_rugosa Rorug03G0110400
rosa_samantha Rh3AG160400 Rh3BG184900 Rh3CG175400 Rh3DG189800
rosa_wichuraiana Rw3G015170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 229
AccII CGCG 3 cut(s) 128, 631, 639
AccIII TCCGGA 1 cut(s) 161
AclWI GGATC 2 cut(s) 8, 452
AcoI YGGCCR 1 cut(s) 980
AcuI CTGAAG 1 cut(s) 483
AfaI GTAC 3 cut(s) 26, 533, 644
AfiI CCNNNNNNNGG 5 cut(s) 102, 127, 128, 161, 702
AflII CTTAAG 1 cut(s) 275
AgsI TTSAA 6 cut(s) 254, 598, 649, 929, 973, 1028
AhdI GACNNNNNGTC 1 cut(s) 993
AjnI CCWGG 1 cut(s) 491
AloI GAACNNNNNNTCC 2 cut(s) 77, 109
AluBI AGCT 7 cut(s) 110, 475, 520, 772, 901, 1052, 1113
AluI AGCT 7 cut(s) 110, 475, 520, 772, 901, 1052, 1113
Alw21I GWGCWC 1 cut(s) 690
Alw26I GTCTC 4 cut(s) 70, 208, 356, 737
Alw44I GTGCAC 1 cut(s) 686
AlwI GGATC 2 cut(s) 8, 452
AlwNI CAGNNNCTG 1 cut(s) 220
Ama87I CYCGRG 1 cut(s) 439
Aor13HI TCCGGA 1 cut(s) 161
AoxI GGCC 6 cut(s) 35, 104, 118, 442, 494, 980
ApaLI GTGCAC 1 cut(s) 686
ApeKI GCWGC 4 cut(s) 862, 898, 1052, 1101
AseI ATTAAT 1 cut(s) 798
Asp700I GAANNNNTTC 1 cut(s) 1032
AspA2I CCTAGG 1 cut(s) 284
AspLEI GCGC 2 cut(s) 213, 639
AspS9I GGNCC 5 cut(s) 35, 104, 119, 442, 625
AsuC2I CCSGG 3 cut(s) 42, 58, 97
AsuHPI GGTGA 2 cut(s) 349, 653
AvaI CYCGRG 1 cut(s) 439
AvaII GGWCC 1 cut(s) 625
AvrII CCTAGG 1 cut(s) 284
BaeGI GKGCMC 3 cut(s) 201, 690, 853
BaeI ACNNNNGTAYC 4 cut(s) 546, 579, 942, 975
BalI TGGCCA 1 cut(s) 982
Bbv12I GWGCWC 1 cut(s) 690
BbvI GCAGC 4 cut(s) 849, 910, 1039, 1088
BccI CCATC 3 cut(s) 176, 496, 719
BceAI ACGGC 1 cut(s) 162
BciT130I CCWGG 1 cut(s) 493
BclI TGATCA 1 cut(s) 580
BcnI CCSGG 3 cut(s) 42, 58, 97
BcoDI GTCTC 4 cut(s) 70, 208, 356, 737
BfaI CTAG 1 cut(s) 285
BfmI CTRYAG 1 cut(s) 727
BfoI RGCGCY 1 cut(s) 214
BfrI CTTAAG 1 cut(s) 275
BfuAI ACCTGC 1 cut(s) 229
BglI GCCNNNNNGGC 1 cut(s) 493
BisI GCNGC 6 cut(s) 38, 155, 863, 899, 1053, 1102
BlnI CCTAGG 1 cut(s) 284
BlpI GCTNAGC 1 cut(s) 768
BlsI GCNGC 6 cut(s) 39, 156, 864, 900, 1054, 1103
BmcAI AGTACT 1 cut(s) 644
Bme1390I CCNGG 4 cut(s) 42, 58, 97, 493
Bme18I GGWCC 1 cut(s) 625
BmeRI GACNNNNNGTC 1 cut(s) 993
BmeT110I CYCGRG 1 cut(s) 439
BmgT120I GGNCC 5 cut(s) 35, 104, 119, 442, 625
BmiI GGNNCC 6 cut(s) 46, 121, 166, 486, 627, 1077
BmrFI CCNGG 4 cut(s) 42, 58, 97, 493
BmrI ACTGGG 1 cut(s) 111
BmsI GCATC 1 cut(s) 434
BmuI ACTGGG 1 cut(s) 111
BpmI CTGGAG 1 cut(s) 1058
Bpu1102I GCTNAGC 1 cut(s) 768
BpuMI CCSGG 3 cut(s) 42, 58, 97
Bsa29I ATCGAT 1 cut(s) 12
BsaI GGTCTC 2 cut(s) 70, 737
BsaJI CCNNGG 4 cut(s) 57, 284, 558, 1015
BsaWI WCCGGW 1 cut(s) 161
BsaXI ACNNNNNCTCC 4 cut(s) 77, 107, 818, 848
Bsc4I CCNNNNNNNGG 5 cut(s) 102, 127, 128, 161, 702
Bse1I ACTGG 4 cut(s) 106, 919, 983, 983
Bse3DI GCAATG 1 cut(s) 762
BseAI TCCGGA 1 cut(s) 161
BseBI CCWGG 1 cut(s) 493
BseCI ATCGAT 1 cut(s) 12
BseDI CCNNGG 4 cut(s) 57, 284, 558, 1015
BseGI GGATG 2 cut(s) 425, 488
BseLI CCNNNNNNNGG 5 cut(s) 102, 127, 128, 161, 702
BseMI GCAATG 1 cut(s) 762
BseMII CTCAG 2 cut(s) 612, 759
BseNI ACTGG 4 cut(s) 106, 919, 983, 983
BseRI GAGGAG 4 cut(s) 389, 482, 485, 839
BseSI GKGCMC 3 cut(s) 201, 690, 853
BseXI GCAGC 4 cut(s) 849, 910, 1039, 1088
Bsh1236I CGCG 3 cut(s) 128, 631, 639
BshFI GGCC 6 cut(s) 37, 106, 120, 444, 496, 982
BshVI ATCGAT 1 cut(s) 12
BsiHKAI GWGCWC 1 cut(s) 690
BsiHKCI CYCGRG 1 cut(s) 439
BsiSI CCGG 4 cut(s) 42, 57, 97, 162
BsiWI CGTACG 1 cut(s) 24
BslI CCNNNNNNNGG 5 cut(s) 102, 127, 128, 161, 702
BsmAI GTCTC 4 cut(s) 70, 208, 356, 737
BsmBI CGTCTC 1 cut(s) 208
BsnI GGCC 6 cut(s) 37, 106, 120, 444, 496, 982
Bso31I GGTCTC 2 cut(s) 70, 737
BsoBI CYCGRG 1 cut(s) 439
Bsp1286I GDGCHC 3 cut(s) 201, 690, 853
Bsp13I TCCGGA 1 cut(s) 161
Bsp143I GATC 4 cut(s) 13, 354, 457, 580
Bsp1720I GCTNAGC 1 cut(s) 768
BspANI GGCC 6 cut(s) 37, 106, 120, 444, 496, 982
BspCNI CTCAG 2 cut(s) 613, 760
BspDI ATCGAT 1 cut(s) 12
BspEI TCCGGA 1 cut(s) 161
BspFNI CGCG 3 cut(s) 128, 631, 639
BspLI GGNNCC 6 cut(s) 46, 121, 166, 486, 627, 1077
BspMI ACCTGC 1 cut(s) 229
BspPI GGATC 2 cut(s) 8, 452
BspTI CTTAAG 1 cut(s) 275
BspTNI GGTCTC 2 cut(s) 70, 737
BsrDI GCAATG 1 cut(s) 762
BsrI ACTGG 4 cut(s) 106, 919, 983, 983
BssECI CCNNGG 4 cut(s) 57, 284, 558, 1015
BssMI GATC 4 cut(s) 13, 354, 457, 580
BssT1I CCWWGG 3 cut(s) 284, 558, 1015
Bst2UI CCWGG 1 cut(s) 493
Bst4CI ACNGT 4 cut(s) 29, 758, 955, 995
Bst6I CTCTTC 1 cut(s) 459
BstAFI CTTAAG 1 cut(s) 275
BstC8I GCNNGC 6 cut(s) 108, 215, 416, 1050, 1099, 1129
BstDEI CTNAG 3 cut(s) 527, 621, 768
BstENI CCTNNNNNAGG 1 cut(s) 700
BstF5I GGATG 2 cut(s) 425, 488
BstFNI CGCG 3 cut(s) 128, 631, 639
BstH2I RGCGCY 1 cut(s) 214
BstHHI GCGC 2 cut(s) 213, 639
BstKTI GATC 4 cut(s) 16, 357, 460, 583
BstMAI GTCTC 4 cut(s) 70, 208, 356, 737
BstMBI GATC 4 cut(s) 13, 354, 457, 580
BstMWI GCNNNNNNNGC 6 cut(s) 68, 493, 502, 868, 1049, 1110
BstNI CCWGG 1 cut(s) 493
BstSCI CCNGG 4 cut(s) 40, 56, 95, 491
BstSFI CTRYAG 1 cut(s) 727
BstSLI GKGCMC 3 cut(s) 201, 690, 853
BstUI CGCG 3 cut(s) 128, 631, 639
BstV1I GCAGC 4 cut(s) 849, 910, 1039, 1088
Bsu15I ATCGAT 1 cut(s) 12
BsuRI GGCC 6 cut(s) 37, 106, 120, 444, 496, 982
BsuTUI ATCGAT 1 cut(s) 12
BtsCI GGATG 2 cut(s) 425, 488
BtsIMutI CAGTG 2 cut(s) 763, 990
BveI ACCTGC 1 cut(s) 229
Cac8I GCNNGC 6 cut(s) 108, 215, 416, 1050, 1099, 1129
CaiI CAGNNNCTG 1 cut(s) 220
CfoI GCGC 2 cut(s) 213, 639
Cfr13I GGNCC 5 cut(s) 35, 104, 119, 442, 625
ClaI ATCGAT 1 cut(s) 12
CseI GACGC 1 cut(s) 293
Csp6I GTAC 3 cut(s) 25, 532, 643
CspCI CAANNNNNGTGG 2 cut(s) 112, 147
CviAII CATG 2 cut(s) 682, 1072
CviQI GTAC 3 cut(s) 25, 532, 643
DdeI CTNAG 3 cut(s) 527, 621, 768
DpnI GATC 4 cut(s) 15, 356, 459, 582
DpnII GATC 4 cut(s) 13, 354, 457, 580
DriI GACNNNNNGTC 1 cut(s) 993
EaeI YGGCCR 1 cut(s) 980
Eam1104I CTCTTC 1 cut(s) 459
Eam1105I GACNNNNNGTC 1 cut(s) 993
EarI CTCTTC 1 cut(s) 459
Eco130I CCWWGG 3 cut(s) 284, 558, 1015
Eco31I GGTCTC 2 cut(s) 70, 737
Eco47I GGWCC 1 cut(s) 625
Eco57I CTGAAG 1 cut(s) 483
Eco88I CYCGRG 1 cut(s) 439
EcoNI CCTNNNNNAGG 1 cut(s) 700
EcoO109I RGGNCCY 1 cut(s) 625
EcoRII CCWGG 1 cut(s) 491
EcoT14I CCWWGG 3 cut(s) 284, 558, 1015
ErhI CCWWGG 3 cut(s) 284, 558, 1015
Esp3I CGTCTC 1 cut(s) 208
FaeI CATG 2 cut(s) 685, 1075
FatI CATG 2 cut(s) 681, 1071
FauI CCCGC 4 cut(s) 56, 121, 636, 1106
FbaI TGATCA 1 cut(s) 580
Fnu4HI GCNGC 6 cut(s) 38, 155, 863, 899, 1053, 1102
FokI GGATG 2 cut(s) 412, 475
Fsp4HI GCNGC 6 cut(s) 38, 155, 863, 899, 1053, 1102
FspBI CTAG 1 cut(s) 285
GlaI GCGC 2 cut(s) 212, 638
GluI GCNGC 6 cut(s) 38, 155, 863, 899, 1053, 1102
GsuI CTGGAG 1 cut(s) 1058
HaeII RGCGCY 1 cut(s) 214
HaeIII GGCC 6 cut(s) 37, 106, 120, 444, 496, 982
HapII CCGG 4 cut(s) 42, 57, 97, 162
HgaI GACGC 1 cut(s) 293
HhaI GCGC 2 cut(s) 213, 639
Hin1II CATG 2 cut(s) 685, 1075
Hin6I GCGC 2 cut(s) 211, 637
HinP1I GCGC 2 cut(s) 211, 637
HinfI GANTC 3 cut(s) 6, 617, 781
HpaII CCGG 4 cut(s) 42, 57, 97, 162
HphI GGTGA 2 cut(s) 349, 653
Hpy166II GTNNAC 2 cut(s) 85, 688
Hpy188I TCNGA 6 cut(s) 208, 268, 547, 622, 999, 1088
Hpy188III TCNNGA 6 cut(s) 82, 162, 371, 461, 703, 1037
Hpy8I GTNNAC 2 cut(s) 85, 688
HpyAV CCTTC 4 cut(s) 27, 168, 706, 994
HpyCH4III ACNGT 4 cut(s) 29, 758, 955, 995
HpyCH4V TGCA 8 cut(s) 238, 425, 505, 688, 809, 865, 912, 1071
HpyF10VI GCNNNNNNNGC 6 cut(s) 68, 493, 502, 868, 1049, 1110
HpyF3I CTNAG 3 cut(s) 527, 621, 768
Hsp92II CATG 2 cut(s) 685, 1075
HspAI GCGC 2 cut(s) 211, 637
Kpn2I TCCGGA 1 cut(s) 161
Ksp22I TGATCA 1 cut(s) 580
Kzo9I GATC 4 cut(s) 13, 354, 457, 580
LmnI GCTCC 4 cut(s) 208, 472, 484, 826
Lsp1109I GCAGC 4 cut(s) 849, 910, 1039, 1088
LweI GCATC 1 cut(s) 434
MaeI CTAG 1 cut(s) 285
MaeIII GTNAC 3 cut(s) 29, 142, 985
MalI GATC 4 cut(s) 15, 356, 459, 582
MboI GATC 4 cut(s) 13, 354, 457, 580
MboII GAAGA 7 cut(s) 12, 105, 476, 515, 650, 661, 1040
MfeI CAATTG 1 cut(s) 879
MhlI GDGCHC 3 cut(s) 201, 690, 853
MlsI TGGCCA 1 cut(s) 982
MluCI AATT 7 cut(s) 52, 233, 254, 297, 320, 795, 879
MluNI TGGCCA 1 cut(s) 982
Mox20I TGGCCA 1 cut(s) 982
MroI TCCGGA 1 cut(s) 161
MroXI GAANNNNTTC 1 cut(s) 1032
MscI TGGCCA 1 cut(s) 982
MseI TTAA 5 cut(s) 276, 300, 384, 399, 798
Msp20I TGGCCA 1 cut(s) 982
MspA1I CMGCKG 3 cut(s) 862, 1066, 1101
MspCI CTTAAG 1 cut(s) 275
MspI CCGG 4 cut(s) 42, 57, 97, 162
MspR9I CCNGG 4 cut(s) 42, 58, 97, 493
MunI CAATTG 1 cut(s) 879
MvaI CCWGG 1 cut(s) 493
MvnI CGCG 3 cut(s) 128, 631, 639
MwoI GCNNNNNNNGC 6 cut(s) 68, 493, 502, 868, 1049, 1110
NciI CCSGG 3 cut(s) 42, 58, 97
NdeII GATC 4 cut(s) 13, 354, 457, 580
NlaIII CATG 2 cut(s) 685, 1075
NlaIV GGNNCC 6 cut(s) 46, 121, 166, 486, 627, 1077
NmeAIII GCCGAG 2 cut(s) 47, 1081
NmuCI GTSAC 1 cut(s) 985
PcsI WCGNNNNNNNCGW 1 cut(s) 132
PdmI GAANNNNTTC 1 cut(s) 1032
PfeI GAWTC 2 cut(s) 617, 781
Pfl23II CGTACG 1 cut(s) 24
PfoI TCCNGGA 1 cut(s) 95
PkrI GCNGC 6 cut(s) 39, 156, 864, 900, 1054, 1103
PpuMI RGGWCCY 1 cut(s) 625
PshBI ATTAAT 1 cut(s) 798
Psp5II RGGWCCY 1 cut(s) 625
Psp6I CCWGG 1 cut(s) 491
PspGI CCWGG 1 cut(s) 491
PspLI CGTACG 1 cut(s) 24
PspN4I GGNNCC 6 cut(s) 46, 121, 166, 486, 627, 1077
PspPI GGNCC 5 cut(s) 35, 104, 119, 442, 625
PspPPI RGGWCCY 1 cut(s) 625
PstNI CAGNNNCTG 1 cut(s) 220
RsaI GTAC 3 cut(s) 26, 533, 644
RsaNI GTAC 3 cut(s) 25, 532, 643
SaqAI TTAA 5 cut(s) 276, 300, 384, 399, 798
SatI GCNGC 6 cut(s) 38, 155, 863, 899, 1053, 1102
Sau3AI GATC 4 cut(s) 13, 354, 457, 580
Sau96I GGNCC 5 cut(s) 35, 104, 119, 442, 625
ScaI AGTACT 1 cut(s) 644
ScrFI CCNGG 4 cut(s) 42, 58, 97, 493
SduI GDGCHC 3 cut(s) 201, 690, 853
SfaNI GCATC 1 cut(s) 434
SfcI CTRYAG 1 cut(s) 727
SinI GGWCC 1 cut(s) 625
SmlI CTYRAG 1 cut(s) 275
SmoI CTYRAG 1 cut(s) 275
Sse9I AATT 7 cut(s) 52, 233, 254, 297, 320, 795, 879
SspMI CTAG 1 cut(s) 285
StyD4I CCNGG 4 cut(s) 40, 56, 95, 491
StyI CCWWGG 3 cut(s) 284, 558, 1015
TaaI ACNGT 4 cut(s) 29, 758, 955, 995
TaqI TCGA 3 cut(s) 12, 171, 258
TaqII GACCGA 1 cut(s) 761
TasI AATT 7 cut(s) 52, 233, 254, 297, 320, 795, 879
TatI WGTACW 1 cut(s) 642
TauI GCSGC 2 cut(s) 40, 157
TfiI GAWTC 2 cut(s) 617, 781
Tru1I TTAA 5 cut(s) 276, 300, 384, 399, 798
Tru9I TTAA 5 cut(s) 276, 300, 384, 399, 798
TscAI CASTG 2 cut(s) 763, 990
TseFI GTSAC 1 cut(s) 985
TseI GCWGC 4 cut(s) 862, 898, 1052, 1101
Tsp45I GTSAC 1 cut(s) 985
TspDTI ATGAA 3 cut(s) 670, 688, 833
TspGWI ACGGA 1 cut(s) 179
TspRI CASTG 2 cut(s) 763, 990
Vha464I CTTAAG 1 cut(s) 275
VneI GTGCAC 1 cut(s) 686
VpaK11BI GGWCC 1 cut(s) 625
VspI ATTAAT 1 cut(s) 798
XagI CCTNNNNNAGG 1 cut(s) 700
XmaJI CCTAGG 1 cut(s) 284
XmnI GAANNNNTTC 1 cut(s) 1032
XspI CTAG 1 cut(s) 285
ZrmI AGTACT 1 cut(s) 644
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.