Rorug03G0110400

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0012260
Physical Location & Seq
Reverse (-)
8834384 .. 8836214
1831 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0110400.1

Sequence Viewer

Length: 1611 bp
ATGGAATCTGCATTTCTTGAGCCTTGGTTTTTCGAAAATTTTATAGACTCATCTTTTGACCAACCTGATTTCATCAACATGGATGATACTCCAATTGATAGCCCAGACTTCTTTTCTCTGTTCAACTCAAATGAAGTTTTCAAAATTTCGGAAATTGCATCAGACCCTTTTGCCACCCTGTTTCCTGGTGATGATCTGATTCAAGTGACATCCCAAGTGGAGGAAGTTTCAATGGATTTGGAAGCATATGAGACTAATTTGATGATAGAGGGTTTCTATGGTAATTCATTGGAAGGGAGTGAAGGGAGTTTTCCATCACAGCAAGCTTCTTCACACCAATTTTCTGCTGAAGGAAATGATGTGTGGAGTCCAAATTCAATAGTGAAGTCTGAAGCATCATCAATGGACTCAACATCAATCCAATCACCACTAAATTTGCCTAGAGAAGAGGAGGGAATCGAGAATCAATTGAGTATTCGTCATCTACTTCAAGCTTTTGCTGAAGCCAAGGAATATGGCCAGAAAGATCTAGAGGAGGTTATTCTCAGCTGCATCAATGAGAAAGTCAGCCCAATTGGAGAGTCTTTCGAGCGCCTTGCATTCAACTTGTGCCAAGAAGTTGTTGATGAACATCAACAAGGCGGCGATTATCTCAAACAAGAATCCTTGAAGAATTTTGAGGCTGCATTTAAGGTGTTCATCCAAAACTTCCCCTATGCTAGGTTTGCTCACTATGTAGCAAACTCAGCAATCCTCGAGGCGATTCCAGCGGAAGCTGAGGCAATTCACATAGTGGATTTCGACATGAGAGAAGGGGTCCAGTTGTCTCAACTTATTGAGGCTTTAGCACAAAGATTCAAAACACTACAAGTGACAGCAATCAAGTGGGAAGAGGAAGAGAGTGATTGTGCTCCTCCACACGGGAGATTTGAGGAGACAAAGAAGCAACTCCAGAACCATGCTAGATCCTTTGGCTTAAACTTAAAGGTGGAAGAAGTAGCAATGGAGGACTTGGTGACTGAGATAAAGAAGGCAAAGAAGAGGGGTGGAGGGAAAGAATTCTTAGCCTTCAACTGTATGGTTTCACTTCCACACATGAGGAGGAGAAGAAGCAGAGGGTATGTCATGGAGTATCTGAGACTGGCTAGGGAGTTGTTAGCCAATTCTGGGAACTCCAAGACTACTAAAAGTGGTATTATAACTTTCGGTGATGGGGATGCTTGTGAGAGACTTGGAAATCACTCGAGTTTCAGCTCATTCTTTAATGAGAATCTAGTGCACTACCAAGCCTTGTTGGAGTCAATGGAGTTGAATTTCCCAAGTCATCTGGCAGAAGCAAGAATGGCGATGGAATGCATCTTCATAGCACCTTTTGTCTCTTCCCAGGCTTGGTTCCAAAAGTGGACAGAAATGAAGGACTGCCATGTTCAACCATGGTTCGGGCTTGAGGGTCGGAGATTGAGCAAAGAAAATTTGATGGAAGCCAAGGACATTGTGGGAGATGAAAGATCCTATGGAATCAGGATTGAAGGACTCAATGGGAATGAAATGGTATTGGAATGGAAAGGAACTCCTCTGGTTAGAGTATCAACTTGGGAAAACCAAAGCTAA

Protein Analysis

536

Amino Acids

60.84

Weight (kDa)

4.68

Isoelectric Point (pI)

48.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 160 - 533 4.1e-44 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012260)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09880 AT5G03470
fragaria_vesca FvH4_6g16640 FvH4_6g16640 FvH4_6g16640
malus_domestica MD04G1090100.v1.1 MD12G1108600.v1.1
prunus_persica Prupe.7G127000_v2.0.a1
pyrus_communis pycom04g08520
rosa_chinensis RchiOBHm_Chr3g0470601
rosa_laevigata RLG00000024255
rosa_multiflora Rmu_sc0002115.1_g000016
rosa_roxburghii Rroxscaffold_6G00410700
rosa_rugosa Rorug03G0110400
rosa_samantha Rh3AG160400 Rh3BG184900 Rh3CG175400 Rh3DG189800
rosa_wichuraiana Rw3G015170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1199
AbsI CCTCGAGG 1 cut(s) 755
AciI CCGC 2 cut(s) 642, 770
AclWI GGATC 2 cut(s) 960, 1503
AcoI YGGCCR 1 cut(s) 517
AcsI RAATTY 8 cut(s) 37, 144, 373, 433, 673, 1058, 1312, 1471
AcuI CTGAAG 3 cut(s) 369, 411, 522
AdeI CACNNNGTG 1 cut(s) 793
AfiI CCNNNNNNNGG 6 cut(s) 220, 720, 920, 1167, 1389, 1439
AjnI CCWGG 2 cut(s) 184, 1383
AluBI AGCT 6 cut(s) 326, 494, 549, 776, 1254, 1608
AluI AGCT 6 cut(s) 326, 494, 549, 776, 1254, 1608
Alw21I GWGCWC 2 cut(s) 913, 1281
Alw26I GTCTC 6 cut(s) 245, 831, 929, 1132, 1222, 1381
Alw44I GTGCAC 1 cut(s) 1277
AlwI GGATC 2 cut(s) 960, 1503
Ama87I CYCGRG 2 cut(s) 755, 1243
AoxI GGCC 1 cut(s) 517
ApaLI GTGCAC 1 cut(s) 1277
ApeKI GCWGC 2 cut(s) 549, 683
ApoI RAATTY 8 cut(s) 37, 144, 373, 433, 673, 1058, 1312, 1471
ArsI GACNNNNNNTTYG 2 cut(s) 38, 70
Asp700I GAANNNNTTC 1 cut(s) 1058
AspLEI GCGC 1 cut(s) 594
AspS9I GGNCC 1 cut(s) 817
AsuHPI GGTGA 4 cut(s) 200, 417, 1027, 1220
AsuII TTCGAA 1 cut(s) 33
AvaI CYCGRG 2 cut(s) 755, 1243
AvaII GGWCC 1 cut(s) 817
BaeGI GKGCMC 1 cut(s) 1281
BalI TGGCCA 1 cut(s) 519
Bbv12I GWGCWC 2 cut(s) 913, 1281
BbvCI CCTCAGC 1 cut(s) 777
BbvI GCAGC 2 cut(s) 536, 670
BccI CCATC 4 cut(s) 322, 1205, 1342, 1471
BcgI CGANNNNNNTGC 2 cut(s) 578, 612
BciT130I CCWGG 2 cut(s) 186, 1385
BcoDI GTCTC 6 cut(s) 245, 831, 929, 1132, 1222, 1381
BfaI CTAG 6 cut(s) 441, 530, 720, 963, 1146, 1274
BfoI RGCGCY 1 cut(s) 595
BglII AGATCT 1 cut(s) 526
BisI GCNGC 3 cut(s) 550, 643, 684
BlsI GCNGC 3 cut(s) 551, 644, 685
Bme1390I CCNGG 2 cut(s) 186, 1385
Bme18I GGWCC 1 cut(s) 817
BmeT110I CYCGRG 2 cut(s) 755, 1243
BmgT120I GGNCC 1 cut(s) 817
BmiI GGNNCC 2 cut(s) 818, 1394
BmrFI CCNGG 2 cut(s) 186, 1385
BmsI GCATC 5 cut(s) 167, 404, 561, 1207, 1365
BpmI CTGGAG 1 cut(s) 935
Bpu10I CCTNAGC 1 cut(s) 777
Bpu14I TTCGAA 1 cut(s) 33
BpuEI CTTGAG 2 cut(s) 38, 1466
BsaBI GATNNNNATC 1 cut(s) 630
BsaJI CCNNGG 5 cut(s) 23, 507, 1383, 1433, 1485
Bsc4I CCNNNNNNNGG 6 cut(s) 220, 720, 920, 1167, 1389, 1439
Bse1I ACTGG 2 cut(s) 820, 1146
Bse3DI GCAATG 1 cut(s) 1008
Bse8I GATNNNNATC 1 cut(s) 630
BseBI CCWGG 2 cut(s) 186, 1385
BseDI CCNNGG 5 cut(s) 23, 507, 1383, 1433, 1485
BseGI GGATG 4 cut(s) 88, 209, 699, 1222
BseJI GATNNNNATC 1 cut(s) 630
BseLI CCNNNNNNNGG 6 cut(s) 220, 720, 920, 1167, 1389, 1439
BseMI GCAATG 1 cut(s) 1008
BseMII CTCAG 5 cut(s) 559, 759, 768, 1011, 1127
BseNI ACTGG 2 cut(s) 820, 1146
BseRI GAGGAG 7 cut(s) 464, 548, 903, 947, 1114, 1117, 1563
BseSI GKGCMC 1 cut(s) 1281
BseXI GCAGC 2 cut(s) 536, 670
BshFI GGCC 1 cut(s) 519
BsiHKAI GWGCWC 2 cut(s) 913, 1281
BsiHKCI CYCGRG 2 cut(s) 755, 1243
BslI CCNNNNNNNGG 6 cut(s) 220, 720, 920, 1167, 1389, 1439
BsmAI GTCTC 6 cut(s) 245, 831, 929, 1132, 1222, 1381
BsmI GAATGC 2 cut(s) 599, 1358
BsnI GGCC 1 cut(s) 519
BsoBI CYCGRG 2 cut(s) 755, 1243
Bsp119I TTCGAA 1 cut(s) 33
Bsp1286I GDGCHC 2 cut(s) 913, 1281
Bsp143I GATC 4 cut(s) 193, 526, 965, 1508
Bsp19I CCATGG 1 cut(s) 1433
BspACI CCGC 2 cut(s) 642, 770
BspANI GGCC 1 cut(s) 519
BspCNI CTCAG 5 cut(s) 558, 758, 769, 1012, 1128
BspLI GGNNCC 2 cut(s) 818, 1394
BspPI GGATC 2 cut(s) 960, 1503
BspT104I TTCGAA 1 cut(s) 33
BsrDI GCAATG 1 cut(s) 1008
BsrI ACTGG 2 cut(s) 820, 1146
BssECI CCNNGG 5 cut(s) 23, 507, 1383, 1433, 1485
BssMI GATC 4 cut(s) 193, 526, 965, 1508
BssT1I CCWWGG 4 cut(s) 23, 507, 1433, 1485
Bst2UI CCWGG 2 cut(s) 186, 1385
Bst4CI ACNGT 1 cut(s) 1076
Bst6I CTCTTC 5 cut(s) 441, 885, 891, 1034, 1384
BstBI TTCGAA 1 cut(s) 33
BstC8I GCNNGC 1 cut(s) 324
BstDEI CTNAG 6 cut(s) 545, 745, 777, 1020, 1063, 1136
BstDSI CCRYGG 1 cut(s) 1433
BstENI CCTNNNNNAGG 1 cut(s) 718
BstF5I GGATG 4 cut(s) 88, 209, 699, 1222
BstH2I RGCGCY 1 cut(s) 595
BstHHI GCGC 1 cut(s) 594
BstKTI GATC 4 cut(s) 196, 529, 968, 1511
BstMAI GTCTC 6 cut(s) 245, 831, 929, 1132, 1222, 1381
BstMBI GATC 4 cut(s) 193, 526, 965, 1508
BstMWI GCNNNNNNNGC 4 cut(s) 725, 746, 767, 1343
BstNI CCWGG 2 cut(s) 186, 1385
BstSCI CCNGG 2 cut(s) 184, 1383
BstSLI GKGCMC 1 cut(s) 1281
BstV1I GCAGC 2 cut(s) 536, 670
BstX2I RGATCY 3 cut(s) 526, 965, 1508
BstYI RGATCY 3 cut(s) 526, 965, 1508
BsuRI GGCC 1 cut(s) 519
BtgI CCRYGG 1 cut(s) 1433
BtgZI GCGATG 1 cut(s) 1361
BtsCI GGATG 4 cut(s) 88, 209, 699, 1222
Cac8I GCNNGC 1 cut(s) 324
CfoI GCGC 1 cut(s) 594
Cfr13I GGNCC 1 cut(s) 817
CspCI CAANNNNNGTGG 2 cut(s) 417, 452
CviAII CATG 7 cut(s) 79, 805, 959, 1096, 1126, 1424, 1434
DdeI CTNAG 6 cut(s) 545, 745, 777, 1020, 1063, 1136
DpnI GATC 4 cut(s) 195, 528, 967, 1510
DpnII GATC 4 cut(s) 193, 526, 965, 1508
DraIII CACNNNGTG 1 cut(s) 793
EaeI YGGCCR 1 cut(s) 517
Eam1104I CTCTTC 5 cut(s) 441, 885, 891, 1034, 1384
EarI CTCTTC 5 cut(s) 441, 885, 891, 1034, 1384
Eco130I CCWWGG 4 cut(s) 23, 507, 1433, 1485
Eco47I GGWCC 1 cut(s) 817
Eco57I CTGAAG 3 cut(s) 369, 411, 522
Eco88I CYCGRG 2 cut(s) 755, 1243
EcoNI CCTNNNNNAGG 1 cut(s) 718
EcoRI GAATTC 1 cut(s) 1058
EcoRII CCWGG 2 cut(s) 184, 1383
EcoT14I CCWWGG 4 cut(s) 23, 507, 1433, 1485
EcoT22I ATGCAT 1 cut(s) 1358
ErhI CCWWGG 4 cut(s) 23, 507, 1433, 1485
FaeI CATG 7 cut(s) 82, 808, 962, 1099, 1129, 1427, 1437
FatI CATG 7 cut(s) 78, 804, 958, 1095, 1125, 1423, 1433
FauNDI CATATG 1 cut(s) 247
Fnu4HI GCNGC 3 cut(s) 550, 643, 684
FokI GGATG 4 cut(s) 95, 196, 686, 1229
Fsp4HI GCNGC 3 cut(s) 550, 643, 684
FspBI CTAG 6 cut(s) 441, 530, 720, 963, 1146, 1274
GlaI GCGC 1 cut(s) 593
GluI GCNGC 3 cut(s) 550, 643, 684
GsuI CTGGAG 1 cut(s) 935
HaeII RGCGCY 1 cut(s) 595
HaeIII GGCC 1 cut(s) 519
HhaI GCGC 1 cut(s) 594
Hin1II CATG 7 cut(s) 82, 808, 962, 1099, 1129, 1427, 1437
Hin6I GCGC 1 cut(s) 592
HinP1I GCGC 1 cut(s) 592
HindIII AAGCTT 2 cut(s) 324, 492
HphI GGTGA 4 cut(s) 200, 417, 1027, 1220
Hpy166II GTNNAC 2 cut(s) 1279, 1404
Hpy188I TCNGA 6 cut(s) 151, 163, 198, 391, 1137, 1455
Hpy188III TCNNGA 5 cut(s) 17, 460, 530, 952, 1522
Hpy8I GTNNAC 2 cut(s) 1279, 1404
HpyAV CCTTC 8 cut(s) 287, 296, 344, 806, 1024, 1078, 1408, 1523
HpyCH4III ACNGT 1 cut(s) 1076
HpyCH4V TGCA 7 cut(s) 11, 158, 552, 599, 686, 1279, 1356
HpyF10VI GCNNNNNNNGC 4 cut(s) 725, 746, 767, 1343
HpyF3I CTNAG 6 cut(s) 545, 745, 777, 1020, 1063, 1136
Hsp92II CATG 7 cut(s) 82, 808, 962, 1099, 1129, 1427, 1437
HspAI GCGC 1 cut(s) 592
Kzo9I GATC 4 cut(s) 193, 526, 965, 1508
LmnI GCTCC 1 cut(s) 916
Lsp1109I GCAGC 2 cut(s) 536, 670
LweI GCATC 5 cut(s) 167, 404, 561, 1207, 1365
MaeI CTAG 6 cut(s) 441, 530, 720, 963, 1146, 1274
MaeIII GTNAC 3 cut(s) 205, 871, 1015
MalI GATC 4 cut(s) 195, 528, 967, 1510
MboI GATC 4 cut(s) 193, 526, 965, 1508
MfeI CAATTG 3 cut(s) 93, 467, 573
MflI RGATCY 3 cut(s) 526, 965, 1508
MhlI GDGCHC 2 cut(s) 913, 1281
MlsI TGGCCA 1 cut(s) 519
MluNI TGGCCA 1 cut(s) 519
MlyI GAGTC 6 cut(s) 41, 376, 401, 590, 1307, 1527
MmeI TCCRAC 2 cut(s) 1275, 1433
Mox20I TGGCCA 1 cut(s) 519
Mph1103I ATGCAT 1 cut(s) 1358
MroXI GAANNNNTTC 1 cut(s) 1058
MscI TGGCCA 1 cut(s) 519
MseI TTAA 4 cut(s) 690, 977, 983, 1263
MslI CAYNNNNRTG 1 cut(s) 77
Msp20I TGGCCA 1 cut(s) 519
MspA1I CMGCKG 2 cut(s) 549, 770
MspR9I CCNGG 2 cut(s) 186, 1385
MunI CAATTG 3 cut(s) 93, 467, 573
Mva1269I GAATGC 2 cut(s) 599, 1358
MvaI CCWGG 2 cut(s) 186, 1385
MwoI GCNNNNNNNGC 4 cut(s) 725, 746, 767, 1343
NcoI CCATGG 1 cut(s) 1433
NdeI CATATG 1 cut(s) 247
NdeII GATC 4 cut(s) 193, 526, 965, 1508
NlaIII CATG 7 cut(s) 82, 808, 962, 1099, 1129, 1427, 1437
NlaIV GGNNCC 2 cut(s) 818, 1394
NmuCI GTSAC 3 cut(s) 205, 871, 1015
NsiI ATGCAT 1 cut(s) 1358
NspV TTCGAA 1 cut(s) 33
PaeR7I CTCGAG 2 cut(s) 755, 1243
PctI GAATGC 2 cut(s) 599, 1358
PdmI GAANNNNTTC 1 cut(s) 1058
PfeI GAWTC 9 cut(s) 5, 199, 456, 463, 662, 763, 855, 1270, 1518
PkrI GCNGC 3 cut(s) 551, 644, 685
PleI GAGTC 6 cut(s) 41, 375, 401, 589, 1306, 1527
PpsI GAGTC 6 cut(s) 41, 375, 401, 589, 1306, 1527
PsiI TTATAA 1 cut(s) 1199
Psp6I CCWGG 2 cut(s) 184, 1383
PspGI CCWGG 2 cut(s) 184, 1383
PspN4I GGNNCC 2 cut(s) 818, 1394
PspPI GGNCC 1 cut(s) 817
PspXI VCTCGAGB 2 cut(s) 755, 1243
PsuI RGATCY 3 cut(s) 526, 965, 1508
PvuII CAGCTG 1 cut(s) 549
RseI CAYNNNNRTG 1 cut(s) 77
SaqAI TTAA 4 cut(s) 690, 977, 983, 1263
SatI GCNGC 3 cut(s) 550, 643, 684
Sau3AI GATC 4 cut(s) 193, 526, 965, 1508
Sau96I GGNCC 1 cut(s) 817
SchI GAGTC 6 cut(s) 41, 376, 401, 590, 1307, 1527
ScrFI CCNGG 2 cut(s) 186, 1385
SduI GDGCHC 2 cut(s) 913, 1281
SfaNI GCATC 5 cut(s) 167, 404, 561, 1207, 1365
Sfr274I CTCGAG 2 cut(s) 755, 1243
SfuI TTCGAA 1 cut(s) 33
SinI GGWCC 1 cut(s) 817
SlaI CTCGAG 2 cut(s) 755, 1243
SmiMI CAYNNNNRTG 1 cut(s) 77
SmlI CTYRAG 4 cut(s) 17, 755, 1243, 1445
SmoI CTYRAG 4 cut(s) 17, 755, 1243, 1445
SsiI CCGC 2 cut(s) 642, 770
SspMI CTAG 6 cut(s) 441, 530, 720, 963, 1146, 1274
StyD4I CCNGG 2 cut(s) 184, 1383
StyI CCWWGG 4 cut(s) 23, 507, 1433, 1485
TaaI ACNGT 1 cut(s) 1076
TaqI TCGA 6 cut(s) 33, 459, 588, 756, 801, 1244
TauI GCSGC 1 cut(s) 645
TfiI GAWTC 9 cut(s) 5, 199, 456, 463, 662, 763, 855, 1270, 1518
Tru1I TTAA 4 cut(s) 690, 977, 983, 1263
Tru9I TTAA 4 cut(s) 690, 977, 983, 1263
TseFI GTSAC 3 cut(s) 205, 871, 1015
TseI GCWGC 2 cut(s) 549, 683
Tsp45I GTSAC 3 cut(s) 205, 871, 1015
TspDTI ATGAA 9 cut(s) 61, 147, 276, 642, 688, 1351, 1427, 1518, 1560
VneI GTGCAC 1 cut(s) 1277
VpaK11BI GGWCC 1 cut(s) 817
XagI CCTNNNNNAGG 1 cut(s) 718
XapI RAATTY 8 cut(s) 37, 144, 373, 433, 673, 1058, 1312, 1471
XbaI TCTAGA 1 cut(s) 529
XcmI CCANNNNNNNNNTGG 1 cut(s) 1492
XhoI CTCGAG 2 cut(s) 755, 1243
XmnI GAANNNNTTC 1 cut(s) 1058
XspI CTAG 6 cut(s) 441, 530, 720, 963, 1146, 1274
Zsp2I ATGCAT 1 cut(s) 1358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.