Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0012260

Physical Location & Seq

Genomic Coordinates Reverse (-)

17160384 .. 17164045

Gene Structure 3662 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1108600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1650 bp

ATGTTTAAGAAAATCATCAAGGGGAACAAGAAGCCCGCGAAATCCGAGACACATGATCCTTCTTCGTACGGGTACGGGCAGCCCGGTACCCGCAATTCTGGGGCGGTCTCGGCGAACGTGGTCGTAAACCATGCCTCCCGCTCTGGGCCGGCTGCTTCCGGCACCAACGCTGTTGGCGCCTCAGGGATTACGGCGTTGCCGCCTTCTGGAACCATCGAGCCTCTCCCTTCGTTCCGTGATGTGCCAGTCTCGGAGCGCCAGACCCTGTTCCTTCGCAAATTGCAGGTTTGTTGCTTTCAATTCGATTTCTCTGATACCCTTAAGAACCCTAGGGAGAAAGAGATTAAGCGTCAAAGCCTTTTGGAATTGGTTGATTTTATACAATCTGGGTCGGGTAAGATCACCGAGACTTGTCAGGAGGAGATGATTAAAATGGTGTCTGTTAATATATTTCGGTGCTTGCCCCCTGCGTCCCATGAAAACACAGGCCAGGAGAATGCCGATCCTGAAGAGGAGGAGCTTTATTTGGAGCCGTCCTGGCCTCATTTGCAGTTGGTTTATGAGCTTCTTCTTAGGTACATTGTGTCATCTGATACGGATACCAAGGTTGCCAAGCGTTATATTGATCACTCATTTGTGTTGAAGTTACTTGATTTGTTTGATTCTGAGGACCCGCGTGAGCGCGAGTACTTGAAAAACAGCCTTCACCGCATATATGGGAAGTTCATGGTGCACCGACCCTTCATCAGGAAAGCCATCAATAACATATTCTATAGGTTTATATATGAGACCGAGAGGCACAGTGGCGTGGCTGAGCTTTTAGAGATTCTTGGGAGTATAATTAATGGGTTTGCTCTTCCAATGAAAGAGGAGCACAAGTTGTTCCTTGTTCGGGCACTTATACCGCTGCATAAGCCGAAGCCAATTGCCGTGTATCACCAGCAGCTGTCCTACTGCATAACCCAGTTTGTTGAAAAGGATTACAAGTTGGCAGATACGGTTGTAAGGGGTTTGTTGAAATACTGGCCGGTGACCAACTGTCAGAAAGAAGTTCTTTTCCTTGGAGAACTTGAAGAAGTTCTGGAGGCAACACAAGCTGCCGAGTTTCAGCGGTGCATGGTTCCTCTTTTCAGACAGATTGCTCGCTGCCTCAATAGCTCTCATTTTCAGGTTTGGGAATATTTTCAAATTTTATTGGGTTTGCTTCATTTAATTATCCATTATGATTATTTGTTCGTATGGAAGACTAGAATTTTATTTTGCTGGAGCTTTAAACTAATACTGACAGACTTAATTTGGGAATTTTACTTGGTGGTATTTATCTTGGTTGCGGAACGAGCACTCTTCTTATGGAATAATGAGCACATTGTCAGTTTAATTGCTCAGAACCGCAATGTCATACTACCAATTATTTTCGAGGCGTTGGAGATGAATATCCAAAATCACTGGAACCAAGCAGTTCACGGGCTGACTGTCAATGTCCGGAAAATGTTTTCGGAAATGGATGCTGAGTTGTTTGAAGACTGCCAGAGGCAGCTTGCAGAGAAAGAAGCGAGCGCAAGAGAGGTGGTAGAACAACGAGAGTTGACATGGAAAAAACTGGCAGACGCGGCAGCACAGAGAGGGGAAGAGGATATGGTTACGAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko03015 ko04071 ko04113 ko04114 ko04151 ko04152 ko04261 ko04728 ko05165 map03015 map04071 map04113 map04114 map04151 map04152 map04261 map04728 map05165

Pfam Domains

Protein Families

B56

Protein Analysis

550

Amino Acids

63.44

Weight (kDa)

6.22

Isoelectric Point (pI)

37.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B56	PF01603	75 - 393	9.8e-150	Protein phosphatase 2A regulatory B subunit (B56 family)
B56	PF01603	443 - 538	9.3e-32	Protein phosphatase 2A regulatory B subunit (B56 family)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012260)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G09880 AT5G03470
fragaria_vesca	FvH4_6g16640 FvH4_6g16640 FvH4_6g16640
malus_domestica	MD04G1090100.v1.1 MD12G1108600.v1.1
prunus_persica	Prupe.7G127000_v2.0.a1
pyrus_communis	pycom04g08520
rosa_chinensis	RchiOBHm_Chr3g0470601
rosa_laevigata	RLG00000024255
rosa_multiflora	Rmu_sc0002115.1_g000016
rosa_roxburghii	Rroxscaffold_6G00410700
rosa_rugosa	Rorug03G0110400
rosa_samantha	Rh3AG160400 Rh3BG184900 Rh3CG175400 Rh3DG189800
rosa_wichuraiana	Rw3G015170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	274
Acc65I	GGTACC	1 cut(s)	86
AccB1I	GGYRCC	3 cut(s)	86, 161, 176
AccBSI	CCGCTC	1 cut(s)	141
AccII	CGCG	4 cut(s)	38, 676, 684, 1610
AccIII	TCCGGA	1 cut(s)	1482
AclWI	GGATC	2 cut(s)	50, 497
AcoI	YGGCCR	1 cut(s)	1025
AcsI	RAATTY	3 cut(s)	1188, 1251, 1301
AcuI	CTGAAG	1 cut(s)	528
AcyI	GRCGYC	1 cut(s)	177
AfaI	GTAC	5 cut(s)	68, 74, 88, 578, 689
AfiI	CCNNNNNNNGG	4 cut(s)	144, 206, 747, 892
AflII	CTTAAG	1 cut(s)	320
AgsI	TTSAA	8 cut(s)	299, 643, 694, 974, 1018, 1073, 1187, 1520
AhdI	GACNNNNNGTC	1 cut(s)	1038
AjnI	CCWGG	2 cut(s)	489, 536
AluBI	AGCT	9 cut(s)	520, 565, 817, 946, 1097, 1158, 1269, 1537, 1647
AluI	AGCT	9 cut(s)	520, 565, 817, 946, 1097, 1158, 1269, 1537, 1647
Alw21I	GWGCWC	4 cut(s)	735, 876, 1342, 1365
Alw26I	GTCTC	5 cut(s)	41, 112, 253, 401, 782
Alw44I	GTGCAC	1 cut(s)	731
AlwI	GGATC	2 cut(s)	50, 497
AlwNI	CAGNNNCTG	2 cut(s)	265, 946
Aor13HI	TCCGGA	1 cut(s)	1482
AoxI	GGCC	4 cut(s)	146, 487, 539, 1025
ApaLI	GTGCAC	1 cut(s)	731
ApeKI	GCWGC	8 cut(s)	79, 152, 907, 943, 1097, 1146, 1534, 1613
ApoI	RAATTY	3 cut(s)	1188, 1251, 1301
AseI	ATTAAT	1 cut(s)	843
Asp700I	GAANNNNTTC	2 cut(s)	1077, 1182
Asp718I	GGTACC	1 cut(s)	86
AspA2I	CCTAGG	1 cut(s)	329
AspLEI	GCGC	4 cut(s)	179, 258, 684, 1559
AspS9I	GGNCC	2 cut(s)	146, 670
AsuC2I	CCSGG	1 cut(s)	84
AsuHPI	GGTGA	4 cut(s)	394, 698, 929, 1042
AvaII	GGWCC	1 cut(s)	670
AvrII	CCTAGG	1 cut(s)	329
AxyI	CCTNAGG	1 cut(s)	181
BaeGI	GKGCMC	2 cut(s)	735, 898
BaeI	ACNNNNGTAYC	4 cut(s)	591, 624, 987, 1020
BanI	GGYRCC	3 cut(s)	86, 161, 176
BbsI	GAAGAC	2 cut(s)	1250, 1527
Bbv12I	GWGCWC	4 cut(s)	735, 876, 1342, 1365
BbvI	GCAGC	8 cut(s)	91, 139, 894, 955, 1084, 1133, 1546, 1625
BccI	CCATC	2 cut(s)	221, 764
BceAI	ACGGC	3 cut(s)	207, 517, 914
BciT130I	CCWGG	2 cut(s)	491, 538
BciVI	GTATCC	1 cut(s)	592
BclI	TGATCA	1 cut(s)	625
BcnI	CCSGG	1 cut(s)	84
BcoDI	GTCTC	5 cut(s)	41, 112, 253, 401, 782
BfaI	CTAG	2 cut(s)	330, 1248
BfmI	CTRYAG	1 cut(s)	772
BfoI	RGCGCY	2 cut(s)	180, 259
BfrI	CTTAAG	1 cut(s)	320
BfuAI	ACCTGC	1 cut(s)	274
BfuI	GTATCC	1 cut(s)	592
BglI	GCCNNNNNGGC	1 cut(s)	538
BlnI	CCTAGG	1 cut(s)	329
BlpI	GCTNAGC	1 cut(s)	813
BmcAI	AGTACT	1 cut(s)	689
Bme1390I	CCNGG	3 cut(s)	84, 491, 538
Bme18I	GGWCC	1 cut(s)	670
BmeRI	GACNNNNNGTC	1 cut(s)	1038
BmgT120I	GGNCC	2 cut(s)	146, 670
BmiI	GGNNCC	8 cut(s)	88, 163, 178, 211, 531, 672, 1122, 1451
BmrFI	CCNGG	3 cut(s)	84, 491, 538
BmrI	ACTGGG	1 cut(s)	958
BmsI	GCATC	1 cut(s)	1495
BmuI	ACTGGG	1 cut(s)	958
BpiI	GAAGAC	2 cut(s)	1250, 1527
BpmI	CTGGAG	2 cut(s)	1103, 1285
Bpu1102I	GCTNAGC	1 cut(s)	813
BpuMI	CCSGG	1 cut(s)	84
BsaBI	GATNNNNATC	1 cut(s)	1433
BsaHI	GRCGYC	1 cut(s)	177
BsaI	GGTCTC	2 cut(s)	112, 782
BsaJI	CCNNGG	3 cut(s)	329, 603, 1060
BsaWI	WCCGGW	1 cut(s)	1482
BsaXI	ACNNNNNCTCC	4 cut(s)	119, 149, 863, 893
Bsc4I	CCNNNNNNNGG	4 cut(s)	144, 206, 747, 892
Bse118I	RCCGGY	2 cut(s)	148, 1027
Bse1I	ACTGG	5 cut(s)	245, 964, 1028, 1451, 1605
Bse21I	CCTNAGG	1 cut(s)	181
Bse3DI	GCAATG	1 cut(s)	1399
Bse8I	GATNNNNATC	1 cut(s)	1433
BseAI	TCCGGA	1 cut(s)	1482
BseBI	CCWGG	2 cut(s)	491, 538
BseDI	CCNNGG	3 cut(s)	329, 603, 1060
BseGI	GGATG	1 cut(s)	1510
BseJI	GATNNNNATC	1 cut(s)	1433
BseLI	CCNNNNNNNGG	4 cut(s)	144, 206, 747, 892
BseMI	GCAATG	1 cut(s)	1399
BseMII	CTCAG	5 cut(s)	195, 657, 804, 1397, 1500
BseNI	ACTGG	5 cut(s)	245, 964, 1028, 1451, 1605
BseRI	GAGGAG	4 cut(s)	434, 527, 530, 884
BseSI	GKGCMC	2 cut(s)	735, 898
BseXI	GCAGC	8 cut(s)	91, 139, 894, 955, 1084, 1133, 1546, 1625
Bsh1236I	CGCG	4 cut(s)	38, 676, 684, 1610
BshFI	GGCC	4 cut(s)	148, 489, 541, 1027
BshNI	GGYRCC	3 cut(s)	86, 161, 176
BsiHKAI	GWGCWC	4 cut(s)	735, 876, 1342, 1365
BsiSI	CCGG	5 cut(s)	84, 149, 159, 1028, 1483
BsiWI	CGTACG	1 cut(s)	66
BslFI	GGGAC	1 cut(s)	457
BslI	CCNNNNNNNGG	4 cut(s)	144, 206, 747, 892
BsmAI	GTCTC	5 cut(s)	41, 112, 253, 401, 782
BsmFI	GGGAC	1 cut(s)	457
BsmI	GAATGC	1 cut(s)	502
BsnI	GGCC	4 cut(s)	148, 489, 541, 1027
Bso31I	GGTCTC	2 cut(s)	112, 782
Bsp1286I	GDGCHC	5 cut(s)	735, 876, 898, 1342, 1365
Bsp13I	TCCGGA	1 cut(s)	1482
Bsp143I	GATC	4 cut(s)	55, 399, 502, 625
Bsp1720I	GCTNAGC	1 cut(s)	813
BspANI	GGCC	4 cut(s)	148, 489, 541, 1027
BspCNI	CTCAG	5 cut(s)	194, 658, 805, 1396, 1501
BspEI	TCCGGA	1 cut(s)	1482
BspFNI	CGCG	4 cut(s)	38, 676, 684, 1610
BspLI	GGNNCC	8 cut(s)	88, 163, 178, 211, 531, 672, 1122, 1451
BspMI	ACCTGC	1 cut(s)	274
BspPI	GGATC	2 cut(s)	50, 497
BspQI	GCTCTTC	1 cut(s)	861
BspT107I	GGYRCC	3 cut(s)	86, 161, 176
BspTI	CTTAAG	1 cut(s)	320
BspTNI	GGTCTC	2 cut(s)	112, 782
BsrBI	CCGCTC	1 cut(s)	141
BsrDI	GCAATG	1 cut(s)	1399
BsrFI	RCCGGY	2 cut(s)	148, 1027
BsrI	ACTGG	5 cut(s)	245, 964, 1028, 1451, 1605
BssAI	RCCGGY	2 cut(s)	148, 1027
BssECI	CCNNGG	3 cut(s)	329, 603, 1060
BssMI	GATC	4 cut(s)	55, 399, 502, 625
BssNI	GRCGYC	1 cut(s)	177
BssT1I	CCWWGG	3 cut(s)	329, 603, 1060
Bst2UI	CCWGG	2 cut(s)	491, 538
Bst4CI	ACNGT	4 cut(s)	803, 1000, 1040, 1474
Bst6I	CTCTTC	4 cut(s)	504, 861, 1349, 1623
BstACI	GRCGYC	1 cut(s)	177
BstAFI	CTTAAG	1 cut(s)	320
BstC8I	GCNNGC	6 cut(s)	36, 150, 461, 1144, 1539, 1555
BstDEI	CTNAG	6 cut(s)	181, 572, 666, 813, 1383, 1509
BstEII	GGTNACC	1 cut(s)	1030
BstENI	CCTNNNNNAGG	1 cut(s)	745
BstF5I	GGATG	1 cut(s)	1510
BstFNI	CGCG	4 cut(s)	38, 676, 684, 1610
BstH2I	RGCGCY	2 cut(s)	180, 259
BstHHI	GCGC	4 cut(s)	179, 258, 684, 1559
BstKTI	GATC	4 cut(s)	58, 402, 505, 628
BstMAI	GTCTC	5 cut(s)	41, 112, 253, 401, 782
BstMBI	GATC	4 cut(s)	55, 399, 502, 625
BstNI	CCWGG	2 cut(s)	491, 538
BstPI	GGTNACC	1 cut(s)	1030
BstSCI	CCNGG	3 cut(s)	82, 489, 536
BstSFI	CTRYAG	1 cut(s)	772
BstSLI	GKGCMC	2 cut(s)	735, 898
BstUI	CGCG	4 cut(s)	38, 676, 684, 1610
BstV1I	GCAGC	8 cut(s)	91, 139, 894, 955, 1084, 1133, 1546, 1625
BstV2I	GAAGAC	2 cut(s)	1250, 1527
Bsu36I	CCTNAGG	1 cut(s)	181
BsuI	GTATCC	1 cut(s)	592
BsuRI	GGCC	4 cut(s)	148, 489, 541, 1027
BtsCI	GGATG	1 cut(s)	1510
BtsIMutI	CAGTG	2 cut(s)	808, 1444
BveI	ACCTGC	1 cut(s)	274
Cac8I	GCNNGC	6 cut(s)	36, 150, 461, 1144, 1539, 1555
CaiI	CAGNNNCTG	2 cut(s)	265, 946
CfoI	GCGC	4 cut(s)	179, 258, 684, 1559
Cfr10I	RCCGGY	2 cut(s)	148, 1027
Cfr13I	GGNCC	2 cut(s)	146, 670
CseI	GACGC	3 cut(s)	338, 459, 1616
Csp6I	GTAC	5 cut(s)	67, 73, 87, 577, 688
CspCI	CAANNNNNGTGG	2 cut(s)	1548, 1583
CviAII	CATG	6 cut(s)	53, 131, 476, 727, 1117, 1590
CviQI	GTAC	5 cut(s)	67, 73, 87, 577, 688
DdeI	CTNAG	6 cut(s)	181, 572, 666, 813, 1383, 1509
DinI	GGCGCC	1 cut(s)	178
DpnI	GATC	4 cut(s)	57, 401, 504, 627
DpnII	GATC	4 cut(s)	55, 399, 502, 625
DraI	TTTAAA	1 cut(s)	1273
DriI	GACNNNNNGTC	1 cut(s)	1038
EaeI	YGGCCR	1 cut(s)	1025
Eam1104I	CTCTTC	4 cut(s)	504, 861, 1349, 1623
Eam1105I	GACNNNNNGTC	1 cut(s)	1038
EarI	CTCTTC	4 cut(s)	504, 861, 1349, 1623
Eco130I	CCWWGG	3 cut(s)	329, 603, 1060
Eco31I	GGTCTC	2 cut(s)	112, 782
Eco47I	GGWCC	1 cut(s)	670
Eco57I	CTGAAG	1 cut(s)	528
Eco81I	CCTNAGG	1 cut(s)	181
Eco91I	GGTNACC	1 cut(s)	1030
EcoNI	CCTNNNNNAGG	1 cut(s)	745
EcoO109I	RGGNCCY	1 cut(s)	670
EcoO65I	GGTNACC	1 cut(s)	1030
EcoRII	CCWGG	2 cut(s)	489, 536
EcoT14I	CCWWGG	3 cut(s)	329, 603, 1060
EgeI	GGCGCC	1 cut(s)	178
EheI	GGCGCC	1 cut(s)	178
ErhI	CCWWGG	3 cut(s)	329, 603, 1060
FaeI	CATG	6 cut(s)	56, 134, 479, 730, 1120, 1593
FalI	AAGNNNNNCTT	2 cut(s)	1038, 1070
FaqI	GGGAC	1 cut(s)	457
FatI	CATG	6 cut(s)	52, 130, 475, 726, 1116, 1589
FauI	CCCGC	4 cut(s)	43, 98, 146, 681
FbaI	TGATCA	1 cut(s)	625
FokI	GGATG	1 cut(s)	1517
FspBI	CTAG	2 cut(s)	330, 1248
GlaI	GCGC	4 cut(s)	178, 257, 683, 1558
GsuI	CTGGAG	2 cut(s)	1103, 1285
HaeII	RGCGCY	2 cut(s)	180, 259
HaeIII	GGCC	4 cut(s)	148, 489, 541, 1027
HapII	CCGG	5 cut(s)	84, 149, 159, 1028, 1483
HgaI	GACGC	3 cut(s)	338, 459, 1616
HhaI	GCGC	4 cut(s)	179, 258, 684, 1559
Hin1I	GRCGYC	1 cut(s)	177
Hin1II	CATG	6 cut(s)	56, 134, 479, 730, 1120, 1593
Hin6I	GCGC	4 cut(s)	177, 256, 682, 1557
HinP1I	GCGC	4 cut(s)	177, 256, 682, 1557
HincII	GTYRAC	1 cut(s)	1587
HindII	GTYRAC	1 cut(s)	1587
HinfI	GANTC	2 cut(s)	662, 826
HpaII	CCGG	5 cut(s)	84, 149, 159, 1028, 1483
HphI	GGTGA	4 cut(s)	394, 698, 929, 1042
Hpy166II	GTNNAC	4 cut(s)	127, 733, 1462, 1587
Hpy188I	TCNGA	9 cut(s)	46, 253, 313, 592, 667, 1044, 1133, 1386, 1498
Hpy188III	TCNNGA	6 cut(s)	207, 416, 506, 748, 1082, 1483
Hpy8I	GTNNAC	4 cut(s)	127, 733, 1462, 1587
HpyAV	CCTTC	6 cut(s)	69, 213, 237, 281, 713, 751
HpyCH4III	ACNGT	4 cut(s)	803, 1000, 1040, 1474
HpyCH4IV	ACGT	1 cut(s)	117
HpyCH4V	TGCA	7 cut(s)	283, 550, 733, 910, 957, 1116, 1541
HpyF3I	CTNAG	6 cut(s)	181, 572, 666, 813, 1383, 1509
HpySE526I	ACGT	1 cut(s)	117
Hsp92I	GRCGYC	1 cut(s)	177
Hsp92II	CATG	6 cut(s)	56, 134, 479, 730, 1120, 1593
HspAI	GCGC	4 cut(s)	177, 256, 682, 1557
KasI	GGCGCC	1 cut(s)	176
Kpn2I	TCCGGA	1 cut(s)	1482
KpnI	GGTACC	1 cut(s)	90
KroI	GCCGGC	1 cut(s)	148
KroNI	GCCGGC	1 cut(s)	150
Ksp22I	TGATCA	1 cut(s)	625
Kzo9I	GATC	4 cut(s)	55, 399, 502, 625
LguI	GCTCTTC	1 cut(s)	861
LmnI	GCTCC	5 cut(s)	253, 517, 529, 871, 1266
Lsp1109I	GCAGC	8 cut(s)	91, 139, 894, 955, 1084, 1133, 1546, 1625
LweI	GCATC	1 cut(s)	1495
MaeI	CTAG	2 cut(s)	330, 1248
MaeII	ACGT	1 cut(s)	117
MaeIII	GTNAC	3 cut(s)	645, 1030, 1639
MalI	GATC	4 cut(s)	57, 401, 504, 627
MbiI	CCGCTC	1 cut(s)	141
MboI	GATC	4 cut(s)	55, 399, 502, 625
MboII	GAAGA	9 cut(s)	54, 521, 560, 848, 1085, 1255, 1336, 1532, 1640
MfeI	CAATTG	1 cut(s)	924
MhlI	GDGCHC	5 cut(s)	735, 876, 898, 1342, 1365
Mly113I	GGCGCC	1 cut(s)	177
MmeI	TCCRAC	1 cut(s)	1404
MroI	TCCGGA	1 cut(s)	1482
MroNI	GCCGGC	1 cut(s)	148
MroXI	GAANNNNTTC	2 cut(s)	1077, 1182
MspA1I	CMGCKG	3 cut(s)	907, 946, 1111
MspCI	CTTAAG	1 cut(s)	320
MspI	CCGG	5 cut(s)	84, 149, 159, 1028, 1483
MspR9I	CCNGG	3 cut(s)	84, 491, 538
MunI	CAATTG	1 cut(s)	924
Mva1269I	GAATGC	1 cut(s)	502
MvaI	CCWGG	2 cut(s)	491, 538
MvnI	CGCG	4 cut(s)	38, 676, 684, 1610
NaeI	GCCGGC	1 cut(s)	150
NarI	GGCGCC	1 cut(s)	177
NciI	CCSGG	1 cut(s)	84
NdeII	GATC	4 cut(s)	55, 399, 502, 625
NgoMIV	GCCGGC	1 cut(s)	148
NlaIII	CATG	6 cut(s)	56, 134, 479, 730, 1120, 1593
NlaIV	GGNNCC	8 cut(s)	88, 163, 178, 211, 531, 672, 1122, 1451
NmeAIII	GCCGAG	2 cut(s)	89, 1126
NmuCI	GTSAC	1 cut(s)	1030
PciSI	GCTCTTC	1 cut(s)	861
PctI	GAATGC	1 cut(s)	502
PdiI	GCCGGC	1 cut(s)	150
PdmI	GAANNNNTTC	2 cut(s)	1077, 1182
PfeI	GAWTC	2 cut(s)	662, 826
Pfl23II	CGTACG	1 cut(s)	66
PluTI	GGCGCC	1 cut(s)	180
PpuMI	RGGWCCY	1 cut(s)	670
PshBI	ATTAAT	1 cut(s)	843
Psp5II	RGGWCCY	1 cut(s)	670
Psp6I	CCWGG	2 cut(s)	489, 536
PspEI	GGTNACC	1 cut(s)	1030
PspGI	CCWGG	2 cut(s)	489, 536
PspLI	CGTACG	1 cut(s)	66
PspN4I	GGNNCC	8 cut(s)	88, 163, 178, 211, 531, 672, 1122, 1451
PspPI	GGNCC	2 cut(s)	146, 670
PspPPI	RGGWCCY	1 cut(s)	670
PstNI	CAGNNNCTG	2 cut(s)	265, 946
PvuII	CAGCTG	1 cut(s)	946
RsaI	GTAC	5 cut(s)	68, 74, 88, 578, 689
RsaNI	GTAC	5 cut(s)	67, 73, 87, 577, 688
SapI	GCTCTTC	1 cut(s)	861
Sau3AI	GATC	4 cut(s)	55, 399, 502, 625
Sau96I	GGNCC	2 cut(s)	146, 670
ScaI	AGTACT	1 cut(s)	689
ScrFI	CCNGG	3 cut(s)	84, 491, 538
SduI	GDGCHC	5 cut(s)	735, 876, 898, 1342, 1365
SfaNI	GCATC	1 cut(s)	1495
SfcI	CTRYAG	1 cut(s)	772
SfoI	GGCGCC	1 cut(s)	178
SinI	GGWCC	1 cut(s)	670
SmlI	CTYRAG	1 cut(s)	320
SmoI	CTYRAG	1 cut(s)	320
SspDI	GGCGCC	1 cut(s)	176
SspI	AATATT	1 cut(s)	1181
SspMI	CTAG	2 cut(s)	330, 1248
StyD4I	CCNGG	3 cut(s)	82, 489, 536
StyI	CCWWGG	3 cut(s)	329, 603, 1060
TaaI	ACNGT	4 cut(s)	803, 1000, 1040, 1474
TaiI	ACGT	1 cut(s)	120
TaqI	TCGA	3 cut(s)	216, 303, 1416
TaqII	GACCGA	1 cut(s)	806
TatI	WGTACW	1 cut(s)	687
TauI	GCSGC	2 cut(s)	202, 1613
TfiI	GAWTC	2 cut(s)	662, 826
TscAI	CASTG	2 cut(s)	808, 1451
TseFI	GTSAC	1 cut(s)	1030
TseI	GCWGC	8 cut(s)	79, 152, 907, 943, 1097, 1146, 1534, 1613
Tsp45I	GTSAC	1 cut(s)	1030
TspDTI	ATGAA	6 cut(s)	492, 715, 733, 878, 1196, 1445
TspGWI	ACGGA	2 cut(s)	224, 611
TspRI	CASTG	2 cut(s)	808, 1451
Vha464I	CTTAAG	1 cut(s)	320
VneI	GTGCAC	1 cut(s)	731
VpaK11BI	GGWCC	1 cut(s)	670
VspI	ATTAAT	1 cut(s)	843
XagI	CCTNNNNNAGG	1 cut(s)	745
XapI	RAATTY	3 cut(s)	1188, 1251, 1301
XmaJI	CCTAGG	1 cut(s)	329
XmnI	GAANNNNTTC	2 cut(s)	1077, 1182
XspI	CTAG	2 cut(s)	330, 1248
ZrmI	AGTACT	1 cut(s)	689

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.